Recombinant AAV Vectors
The Adeno-Associated Virus (AAV) is the most promising candidate for virus-based gene therapy as it:
- Infects both dividing and non-dividing cells
- Has low immunogenicity and pathogenicity
- Can target a broad range of specific tissue types
- Provides long-term expression in non-dividing cells
Our complete library of premade AAV vectors and prepackaged AAV particles for human,mouse,and rat genes come with a wide selection of promoters and reporters for every need. For gene knockdowns, access our AAV-siRNA vectors and viruses as well as our AAV-miRNA over-expression and inhibition tools.
| Cloning Vector | Application | Promoter | Reporter | Ready-to-use AAV |
|---|---|---|---|---|
| CRISPR | ||||
| pAAV-PGK-saCas9-U6-sgRNAsa-hGH-amp | All-in-One sgRNA/saCas9 | U6 | C306 | |
| pAAV-U6-sgRNAsa-GFP-hGH-amp | sgRNA (for saCas9) | U6 | GFP | C304 |
| pAAV-U6-sgRNAsp-GFP-hGH-amp | sgRNA (for spCas9) | U6 | GFP | C302 |
| cDNA expression | ||||
| pAAV-G-CMV-amp | cDNA expression | CMV | AAV001a | |
| pAAV-G-CMV-CBH-gcGFP-hGH-amp | cDNA expression | CMV | GFP | AAV003a |
| pAAV-G-CMV-SV40-Luc-hGH-amp | cDNA expression | CMV | Luc | AAV002a |
| pAAV-G-hSyn-hGH-amp | cDNA expression | hSyn | AAV022a | |
| pAAV-G-PGK-hGH-amp | cDNA expression | PGK | AAV004a | |
| pAAV-G-EF1a-hGH-amp | cDNA expression | EF1a | AAV007a | |
| pAAV-G-CAGGS-hGH-amp | cDNA expression | CAGGS | AAV013a | |
| *GFP and Luc versions are also available for non-CMV promoter vectors | ||||
| siRNA | ||||
| pAAV-siRNA-GFP-hGH-amp | siRNA | U6, H1 | GFP | C519 |
| pAAV-shRNA-GFP-hGH-amp | siRNA | U6, H1 | GFP | C518 |
| miRNA | ||||
| pAAV-mir-GFP-hGH-amp | pre-miRNA | CMV | GFP | Am00100a |
| pAAV-mico-GFP-hGH-amp | mature miRNA | CMV | GFP | Am00101a |
| pAAV-mir-OFF-hGH-amp | miRNA inhibitor | H1 | GFP | Am00700a |
Choosing the right promoter
| Promoter | Applications |
|---|---|
| CMV (Strong) | A widely used promoter that provides strong gene expression in most cells (weak activity in human and mouse stem cells). |
| EF1α | Strong expression promoter; highly efficient in stem cells. Good for stable expression from long term cultures. |
| PGK (Medium) | A high efficiency promoter able to sustain stable activity in long term cultures of undifferentiated stem cells. |
| CAGGS (Strong) | A large hybrid promoter (composed of the CMV immediate-early enhancer as well as the CBA and CBA intron 1/exon 1 promoter) with no methylation issues (max.insert size:2kb). |
| hSyn (Medium-strong) | The synapsin I promoter; has been used to acheive highly neuron-specific long-term transgene expression in vivo. |