pAAV-G-CAGGS-hGH-amp
| Vector Name | pAAV-G-CAGGS-hGH-amp |
| VectorType | AAV Plasmid |
| Antibiotic Information | Ampicillin |
| Sequencing Primers | mPGK-Forward: 5'--CATTCTGCACGCTTCAAAAG--3' |
| Additional Information | No Additional Information |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgccc taggccggcc acgcgtggcg cgttgacatt gattattgac
181 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
241 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
301 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
361 atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
421 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
481 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
541 catgggtcga ggtgagcccc acgttctgct tcactctccc catctccccc ccctccccac
601 ccccaatttt gtatttattt attttttaat tattttgtgc agcgatgggg gcgggggggg
661 gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg gggcggggcg aggcggagag
721 gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt tccttttatg gcgaggcggc
781 ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc gggagtcgct gcgttgcctt
841 cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt
901 tactcccaca ggtgagcggg cgggacggcc cttctcctcc gggctgtaat tagcgcttgg
961 tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag ccttaaaggg ctccgggagg
1021 gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc
1081 gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg cgggcgcggc gcggggcttt
1141 gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg
1201 ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg tgggggggtg agcagggggt
1261 gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc cctccccgag ttgctgagca
1321 cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc gcggggctcg ccgtgccggg
1381 cggggggtgg cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg ccggggaggg
1441 ctcgggggag gggcgcggcg gccccggagc gccggcggct gtcgaggcgc ggcgagccgc
1501 agccattgcc ttttatggta atcgtgcgag agggcgcagg gacttccttt gtcccaaatc
1561 tggcggagcc gaaatctggg aggcgccgcc gcaccccctc tagcgggcgc gggcgaagcg
1621 gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg
1681 tccccttctc catctccagc ctcggggctg ccgcaggggg acggctgcct tcggggggga
1741 cggggcaggg cggggttcgg cttctggcgt gtgaccggcg gctctagagc ctctgctaac
1801 catgttcatg ccttcttctt tttcctacag ctcctgggca acgtgctggt tgttgtgctg
1861 tctcatcatt ttggcaaaga attggcgcgc cgagctcgtt tagtgaaccg tcagatcgcc
1921 tggagacgcc atccacgctg ttttgacctc catagaagaa ccgagtttaa actccctatc
1981 agtgatagag atctccctat cagtgataga gagctagcga tatcaacaag tttgtacaaa
2041 aaagctgaac gagaaacgta aaatgatata aatatcaata tattaaatta gattttgcat
2101 aaaaaacaga ctacataata ctgtaaaaca caacatatcc agtcactatg gcggccgcat
2161 taggcacccc aggctttaca ctttatgctt ccggctcgta taatgtgtgg attttgagtt
2221 aggatccgtc gagattttca ggagctaagg aagctaaaat ggagaaaaaa atcactggat
2281 ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca tttcagtcag
2341 ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt ttaaagaccg
2401 taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc cgcctgatga
2461 atgctcatcc ggaattccgt atggcaatga aagacggtga gctggtgata tgggatagtg
2521 ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg ctctggagtg
2581 aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg gcgtgttacg
2641 gtgaaaacct ggcctatttc cctaaagggt ttattgagaa tatgtttttc gtctcagcca
2701 atccctgggt gagtttcacc agttttgatt taaacgtggc caatatggac aacttcttcg
2761 cccccgtttt caccatgggc aaatattata cgcaaggcga caaggtgctg atgccgctgg
2821 cgattcaggt tcatcatgcc gtttgtgatg gcttccatgt cggcagaatg cttaatgaat
2881 tacaacagta ctgcgatgag tggcagggcg gggcgtaaag atctggatcc ggcttactaa
2941 aagccagata acagtatgcg tatttgcgcg ctgatttttg cggtataaga atatatactg
3001 atatgtatac ccgaagtatg tcaaaaagag gtatgctatg aagcagcgta ttacagtgac
3061 agttgacagc gacagctatc agttgctcaa ggcatatatg atgtcaatat ctccggtctg
3121 gtaagcacaa ccatgcagaa tgaagcccgt cgtctgcgtg ccgaacgctg gaaagcggaa
3181 aatcaggaag ggatggctga ggtcgcccgg tttattgaaa tgaacggctc ttttgctgac
3241 gagaacaggg gctggtgaaa tgcagtttaa ggtttacacc tataaaagag agagccgtta
3301 tcgtctgttt gtggatgtac agagtgatat tattgacacg cccgggcgac ggatggtgat
3361 ccccctggcc agtgcacgtc tgctgtcaga taaagtctcc cgtgaacttt acccggtggt
3421 gcatatcggg gatgaaagct ggcgcatgat gaccaccgat atggccagtg tgccggtctc
3481 cgttatcggg gaagaagtgg ctgatctcag ccaccgcgaa aatgacatca aaaacgccat
3541 taacctgatg ttctggggaa tataaatgtc aggctccctt atacacagcc agtctgcagg
3601 tcgaccatag tgactggata tgttgtgttt tacagtatta tgtagtctgt tttttatgca
3661 aaatctaatt taatatattg atatttatat cattttacgt ttctcgttca gctttcttgt
3721 acaaagtggt tgatatctga ctcgagccaa tcttaagggg tggcatccct gtgacccctc
3781 cccagtgcct ctcctggccc tggaagttgc cactccagtg cccaccagcc ttgtcctaat
3841 aaaattaagt tgcatcattt tgtctgacta ggtgtccttc tataatatta tggggtggag
3901 gggggtggta tggagcaagg ggcaagttgg gaagacaacc tgtagggcct gcggggtcta
3961 ttgggaacca agctggagtg cagtggcaca atcttggctc actgcaatct ccgcctcctg
4021 ggttcaagcg attctcctgc ctcagcctcc cgagttgttg ggattccagg catgcatgac
4081 caggctcagc taatttttgt ttttttggta gagacggggt ttcaccatat tggccaggct
4141 ggtctccaac tcctaatctc aggtgatcta cccaccttgg cctcccaaat tgctgggatt
4201 acaggcgtga accactgctc ccttccctgt ccttcacgtg cggaccgagc ggccgcagga
4261 acccctagtg atggagttgg ccactccctc tctgcgcgct cgctcgctca ctgaggccgg
4321 gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc
4381 gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
4441 ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc attaagcgcg
4501 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgcctt agcgcccgct
4561 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
4621 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
4681 cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
4741 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
4801 aactctatct cgggctattc ttttgattta taagggattt tgccgatttc ggtctattgg
4861 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt
4921 acaattttat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc
4981 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
5041 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
5101 ccgaaacgcg cgagccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
5161 cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
5221 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
5281 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
5341 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
5401 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
5461 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
5521 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
5581 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
5641 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
5701 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
5761 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
5821 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
5881 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
5941 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
6001 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
6061 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
6121 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
6181 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
6241 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
6301 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
6361 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
6421 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
6481 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
6541 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
6601 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
6661 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
6721 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
6781 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
6841 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
6901 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
6961 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
7021 cttttgctgg ccttttgctc acatgt
//
LOCUS dna 7046 bp
FEATURES Location/Qualifiers
misc_binding 413..418
/dbxref="REBASE:NdeI"
Promoter 545..822
/gene="cBA prom"
ORF 962..1693
/sequence="ORF_3 rf(5)"
misc_binding 1019..1024
/dbxref="REBASE:ApaI"
misc_binding 1783..1788
/dbxref="REBASE:XbaI"
misc_binding 1892..1897
/dbxref="REBASE:SacI"
Regulatory_Seq 1973..2012
/gene="tetO reg"
Other Gene 2026..2150
/gene="attR1 other"
Other Gene 2050..2150
/gene="attR2 other"
Marker 2259..2918
/gene="CAT marker"
ORF 2259..2918
/sequence="ORF_2 rf(3)"
misc_binding 2472..2477
/dbxref="REBASE:EcoRI"
Other Gene 3260..3565
/gene="ccdB other"
Other Gene 3606..3725
/gene="attR1 other"
Other Gene 3606..3706
/gene="attR2 other"
misc_binding 3741..3746
/dbxref="REBASE:XhoI"
Terminator 3758..4234
/gene="hGH_PA term"
misc_binding 4070..4075
/dbxref="REBASE:SphI"
Rep_Origin 4489..4795
/gene="f1 origin"
Promoter 5296..5324
/gene="amp prom"
Marker 5366..6226
/gene="amp marker"
ORF 5366..6226
/sequence="ORF_1 rf(2)"
Rep_Origin 6381..7000
/gene="pBR322 origin"
BASE COUNT 1540 a 1793 c 1976 g 1737 t 0 others
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgccc taggccggcc acgcgtggcg cgttgacatt gattattgac
181 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
241 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
301 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
361 atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
421 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
481 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
541 catgggtcga ggtgagcccc acgttctgct tcactctccc catctccccc ccctccccac
601 ccccaatttt gtatttattt attttttaat tattttgtgc agcgatgggg gcgggggggg
661 gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg gggcggggcg aggcggagag
721 gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt tccttttatg gcgaggcggc
781 ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc gggagtcgct gcgttgcctt
841 cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt
901 tactcccaca ggtgagcggg cgggacggcc cttctcctcc gggctgtaat tagcgcttgg
961 tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag ccttaaaggg ctccgggagg
1021 gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc
1081 gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg cgggcgcggc gcggggcttt
1141 gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg
1201 ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg tgggggggtg agcagggggt
1261 gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc cctccccgag ttgctgagca
1321 cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc gcggggctcg ccgtgccggg
1381 cggggggtgg cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg ccggggaggg
1441 ctcgggggag gggcgcggcg gccccggagc gccggcggct gtcgaggcgc ggcgagccgc
1501 agccattgcc ttttatggta atcgtgcgag agggcgcagg gacttccttt gtcccaaatc
1561 tggcggagcc gaaatctggg aggcgccgcc gcaccccctc tagcgggcgc gggcgaagcg
1621 gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg
1681 tccccttctc catctccagc ctcggggctg ccgcaggggg acggctgcct tcggggggga
1741 cggggcaggg cggggttcgg cttctggcgt gtgaccggcg gctctagagc ctctgctaac
1801 catgttcatg ccttcttctt tttcctacag ctcctgggca acgtgctggt tgttgtgctg
1861 tctcatcatt ttggcaaaga attggcgcgc cgagctcgtt tagtgaaccg tcagatcgcc
1921 tggagacgcc atccacgctg ttttgacctc catagaagaa ccgagtttaa actccctatc
1981 agtgatagag atctccctat cagtgataga gagctagcga tatcaacaag tttgtacaaa
2041 aaagctgaac gagaaacgta aaatgatata aatatcaata tattaaatta gattttgcat
2101 aaaaaacaga ctacataata ctgtaaaaca caacatatcc agtcactatg gcggccgcat
2161 taggcacccc aggctttaca ctttatgctt ccggctcgta taatgtgtgg attttgagtt
2221 aggatccgtc gagattttca ggagctaagg aagctaaaat ggagaaaaaa atcactggat
2281 ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca tttcagtcag
2341 ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt ttaaagaccg
2401 taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc cgcctgatga
2461 atgctcatcc ggaattccgt atggcaatga aagacggtga gctggtgata tgggatagtg
2521 ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg ctctggagtg
2581 aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg gcgtgttacg
2641 gtgaaaacct ggcctatttc cctaaagggt ttattgagaa tatgtttttc gtctcagcca
2701 atccctgggt gagtttcacc agttttgatt taaacgtggc caatatggac aacttcttcg
2761 cccccgtttt caccatgggc aaatattata cgcaaggcga caaggtgctg atgccgctgg
2821 cgattcaggt tcatcatgcc gtttgtgatg gcttccatgt cggcagaatg cttaatgaat
2881 tacaacagta ctgcgatgag tggcagggcg gggcgtaaag atctggatcc ggcttactaa
2941 aagccagata acagtatgcg tatttgcgcg ctgatttttg cggtataaga atatatactg
3001 atatgtatac ccgaagtatg tcaaaaagag gtatgctatg aagcagcgta ttacagtgac
3061 agttgacagc gacagctatc agttgctcaa ggcatatatg atgtcaatat ctccggtctg
3121 gtaagcacaa ccatgcagaa tgaagcccgt cgtctgcgtg ccgaacgctg gaaagcggaa
3181 aatcaggaag ggatggctga ggtcgcccgg tttattgaaa tgaacggctc ttttgctgac
3241 gagaacaggg gctggtgaaa tgcagtttaa ggtttacacc tataaaagag agagccgtta
3301 tcgtctgttt gtggatgtac agagtgatat tattgacacg cccgggcgac ggatggtgat
3361 ccccctggcc agtgcacgtc tgctgtcaga taaagtctcc cgtgaacttt acccggtggt
3421 gcatatcggg gatgaaagct ggcgcatgat gaccaccgat atggccagtg tgccggtctc
3481 cgttatcggg gaagaagtgg ctgatctcag ccaccgcgaa aatgacatca aaaacgccat
3541 taacctgatg ttctggggaa tataaatgtc aggctccctt atacacagcc agtctgcagg
3601 tcgaccatag tgactggata tgttgtgttt tacagtatta tgtagtctgt tttttatgca
3661 aaatctaatt taatatattg atatttatat cattttacgt ttctcgttca gctttcttgt
3721 acaaagtggt tgatatctga ctcgagccaa tcttaagggg tggcatccct gtgacccctc
3781 cccagtgcct ctcctggccc tggaagttgc cactccagtg cccaccagcc ttgtcctaat
3841 aaaattaagt tgcatcattt tgtctgacta ggtgtccttc tataatatta tggggtggag
3901 gggggtggta tggagcaagg ggcaagttgg gaagacaacc tgtagggcct gcggggtcta
3961 ttgggaacca agctggagtg cagtggcaca atcttggctc actgcaatct ccgcctcctg
4021 ggttcaagcg attctcctgc ctcagcctcc cgagttgttg ggattccagg catgcatgac
4081 caggctcagc taatttttgt ttttttggta gagacggggt ttcaccatat tggccaggct
4141 ggtctccaac tcctaatctc aggtgatcta cccaccttgg cctcccaaat tgctgggatt
4201 acaggcgtga accactgctc ccttccctgt ccttcacgtg cggaccgagc ggccgcagga
4261 acccctagtg atggagttgg ccactccctc tctgcgcgct cgctcgctca ctgaggccgg
4321 gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc
4381 gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
4441 ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc attaagcgcg
4501 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgcctt agcgcccgct
4561 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
4621 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
4681 cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
4741 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
4801 aactctatct cgggctattc ttttgattta taagggattt tgccgatttc ggtctattgg
4861 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt
4921 acaattttat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc
4981 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
5041 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
5101 ccgaaacgcg cgagccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
5161 cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
5221 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
5281 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
5341 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
5401 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
5461 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
5521 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
5581 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
5641 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
5701 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
5761 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
5821 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
5881 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
5941 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
6001 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
6061 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
6121 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
6181 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
6241 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
6301 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
6361 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
6421 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
6481 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
6541 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
6601 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
6661 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
6721 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
6781 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
6841 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
6901 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
6961 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
7021 cttttgctgg ccttttgctc acatgt
//
***Note: The text map shown below is representative of the parental
vector only and does not include the gene of interest. The sequence of the gene
can be found through the accession number link on the product page.
1 cctgcaggcagctgcgcgctcgctcgctcactgaggccgcccgggcgtcgggcgaccttt 60
GGACGTCCGTCGACGCGCGAGCGAGCGAGTGACTCCGGCGGGCCCGCAGCCCGCTGGAAA
61 ggtcgcccggcctcagtgagcgagcgagcgcgcagagagggagtggccaactccatcact 120
CCAGCGGGCCGGAGTCACTCGCTCGCTCGCGCGTCTCTCCCTCACCGGTTGAGGTAGTGA
121 aggggttcctgcggccgccctaggccggccacgcgtggcgcgttgacattgattattgac 180
TCCCCAAGGACGCCGGCGGGATCCGGCCGGTGCGCACCGCGCAACTGTAACTAATAACTG
181 tagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccg 240
ATCAATAATTATCATTAGTTAATGCCCCAGTAATCAAGTATCGGGTATATACCTCAAGGC
241 cgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccatt 300
GCAATGTATTGAATGCCATTTACCGGGCGGACCGACTGGCGGGTTGCTGGGGGCGGGTAA
301 gacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtca 360
CTGCAGTTATTACTGCATACAAGGGTATCATTGCGGTTATCCCTGAAAGGTAACTGCAGT
NdeI
|
361 atgggtggactatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgcc 420
TACCCACCTGATAAATGCCATTTGACGGGTGAACCGTCATGTAGTTCACATAGTATACGG
421 aagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagta 480
TTCATGCGGGGGATAACTGCAGTTACTGCCATTTACCGGGCGGACCGTAATACGGGTCAT
481 catgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattac 540
GTACTGGAATACCCTGAAAGGATGAACCGTCATGTAGATGCATAATCAGTAGCGATAATG
cBA prom(545,822)>>>
|
541 catgggtcgaggtgagccccacgttctgcttcactctccccatctcccccccctccccac 600
GTACCCAGCTCCACTCGGGGTGCAAGACGAAGTGAGAGGGGTAGAGGGGGGGGAGGGGTG
601 ccccaattttgtatttatttattttttaattattttgtgcagcgatgggggcgggggggg 660
GGGGTTAAAACATAAATAAATAAAAAATTAATAAAACACGTCGCTACCCCCGCCCCCCCC
661 gggggcgcgcgccaggcggggcggggcggggcgaggggcggggcggggcgaggcggagag 720
CCCCCGCGCGCGGTCCGCCCCGCCCCGCCCCGCTCCCCGCCCCGCCCCGCTCCGCCTCTC
721 gtgcggcggcagccaatcagagcggcgcgctccgaaagtttccttttatggcgaggcggc 780
CACGCCGCCGTCGGTTAGTCTCGCCGCGCGAGGCTTTCAAAGGAAAATACCGCTCCGCCG
781 ggcggcggcggccctataaaaagcgaagcgcgcggcgggcgggagtcgctgcgttgcctt 840
CCGCCGCCGCCGGGATATTTTTCGCTTCGCGCGCCGCCCGCCCTCAGCGACGCAACGGAA
841 cgccccgtgccccgctccgcgccgcctcgcgccgcccgccccggctctgactgaccgcgt 900
GCGGGGCACGGGGCGAGGCGCGGCGGAGCGCGGCGGGCGGGGCCGAGACTGACTGGCGCA
901 tactcccacaggtgagcgggcgggacggcccttctcctccgggctgtaattagcgcttgg 960
ATGAGGGTGTCCACTCGCCCGCCCTGCCGGGAAGAGGAGGCCCGACATTAATCGCGAACC
ORF_3 rf(5)(962,1693)<<<
|
961 tttaatgacggctcgtttcttttctgtggctgcgtgaaagccttaaagggctccgggagg 1020
AAATTACTGCCGAGCAAAGAAAAGACACCGACGCACTTTCGGAATTTCCCGAGGCCCTCC
ApaI
|
1021 gccctttgtgcgggggggagcggctcggggggtgcgtgcgtgtgtgtgtgcgtggggagc 1080
CGGGAAACACGCCCCCCCTCGCCGAGCCCCCCACGCACGCACACACACACGCACCCCTCG
1081 gccgcgtgcggcccgcgctgcccggcggctgtgagcgctgcgggcgcggcgcggggcttt 1140
CGGCGCACGCCGGGCGCGACGGGCCGCCGACACTCGCGACGCCCGCGCCGCGCCCCGAAA
1141 gtgcgctccgcgtgtgcgcgaggggagcgcggccgggggcggtgccccgcggtgcggggg 1200
CACGCGAGGCGCACACGCGCTCCCCTCGCGCCGGCCCCCGCCACGGGGCGCCACGCCCCC
1201 ggctgcgaggggaacaaaggctgcgtgcggggtgtgtgcgtgggggggtgagcagggggt 1260
CCGACGCTCCCCTTGTTTCCGACGCACGCCCCACACACGCACCCCCCCACTCGTCCCCCA
1261 gtgggcgcggcggtcgggctgtaacccccccctgcacccccctccccgagttgctgagca 1320
CACCCGCGCCGCCAGCCCGACATTGGGGGGGGACGTGGGGGGAGGGGCTCAACGACTCGT
1321 cggcccggcttcgggtgcggggctccgtgcggggcgtggcgcggggctcgccgtgccggg 1380
GCCGGGCCGAAGCCCACGCCCCGAGGCACGCCCCGCACCGCGCCCCGAGCGGCACGGCCC
1381 cggggggtggcggcaggtgggggtgccgggcggggcggggccgcctcgggccggggaggg 1440
GCCCCCCACCGCCGTCCACCCCCACGGCCCGCCCCGCCCCGGCGGAGCCCGGCCCCTCCC
1441 ctcgggggaggggcgcggcggccccggagcgccggcggctgtcgaggcgcggcgagccgc 1500
GAGCCCCCTCCCCGCGCCGCCGGGGCCTCGCGGCCGCCGACAGCTCCGCGCCGCTCGGCG
1501 agccattgccttttatggtaatcgtgcgagagggcgcagggacttcctttgtcccaaatc 1560
TCGGTAACGGAAAATACCATTAGCACGCTCTCCCGCGTCCCTGAAGGAAACAGGGTTTAG
1561 tggcggagccgaaatctgggaggcgccgccgcaccccctctagcgggcgcgggcgaagcg 1620
ACCGCCTCGGCTTTAGACCCTCCGCGGCGGCGTGGGGGAGATCGCCCGCGCCCGCTTCGC
1621 gtgcggcgccggcaggaaggaaatgggcggggagggccttcgtgcgtcgccgcgccgccg 1680
CACGCCGCGGCCGTCCTTCCTTTACCCGCCCCTCCCGGAAGCACGCAGCGGCGCGGCGGC
1681 tccccttctccatctccagcctcggggctgccgcagggggacggctgccttcggggggga 1740
AGGGGAAGAGGTAGAGGTCGGAGCCCCGACGGCGTCCCCCTGCCGACGGAAGCCCCCCCT
XbaI
|
1741 cggggcagggcggggttcggcttctggcgtgtgaccggcggctctagagcctctgctaac 1800
GCCCCGTCCCGCCCCAAGCCGAAGACCGCACACTGGCCGCCGAGATCTCGGAGACGATTG
1801 catgttcatgccttcttctttttcctacagctcctgggcaacgtgctggttgttgtgctg 1860
GTACAAGTACGGAAGAAGAAAAAGGATGTCGAGGACCCGTTGCACGACCAACAACACGAC
SacI
|
1861 tctcatcattttggcaaagaattggcgcgccgagctcgtttagtgaaccgtcagatcgcc 1920
AGAGTAGTAAAACCGTTTCTTAACCGCGCGGCTCGAGCAAATCACTTGGCAGTCTAGCGG
tetO reg(1973,2012)>>>
|
1921 tggagacgccatccacgctgttttgacctccatagaagaaccgagtttaaactccctatc 1980
ACCTCTGCGGTAGGTGCGACAAAACTGGAGGTATCTTCTTGGCTCAAATTTGAGGGATAG
attR1 other(2026,2150)>>>
|
1981 agtgatagagatctccctatcagtgatagagagctagcgatatcaacaagtttgtacaaa 2040
TCACTATCTCTAGAGGGATAGTCACTATCTCTCGATCGCTATAGTTGTTCAAACATGTTT
attR2 other(2050,2150)<<<
|
2041 aaagctgaacgagaaacgtaaaatgatataaatatcaatatattaaattagattttgcat 2100
TTTCGACTTGCTCTTTGCATTTTACTATATTTATAGTTATATAATTTAATCTAAAACGTA
2101 aaaaaacagactacataatactgtaaaacacaacatatccagtcactatggcggccgcat 2160
TTTTTTGTCTGATGTATTATGACATTTTGTGTTGTATAGGTCAGTGATACCGCCGGCGTA
2161 taggcaccccaggctttacactttatgcttccggctcgtataatgtgtggattttgagtt 2220
ATCCGTGGGGTCCGAAATGTGAAATACGAAGGCCGAGCATATTACACACCTAAAACTCAA
CAT marker(2259,2918)>>>
|
ORF_2 rf(3)(2259,2918)>>>
|
2221 aggatccgtcgagattttcaggagctaaggaagctaaaatggagaaaaaaatcactggat 2280
TCCTAGGCAGCTCTAAAAGTCCTCGATTCCTTCGATTTTACCTCTTTTTTTAGTGACCTA
2281 ataccaccgttgatatatcccaatggcatcgtaaagaacattttgaggcatttcagtcag 2340
TATGGTGGCAACTATATAGGGTTACCGTAGCATTTCTTGTAAAACTCCGTAAAGTCAGTC
2341 ttgctcaatgtacctataaccagaccgttcagctggatattacggcctttttaaagaccg 2400
AACGAGTTACATGGATATTGGTCTGGCAAGTCGACCTATAATGCCGGAAAAATTTCTGGC
2401 taaagaaaaataagcacaagttttatccggcctttattcacattcttgcccgcctgatga 2460
ATTTCTTTTTATTCGTGTTCAAAATAGGCCGGAAATAAGTGTAAGAACGGGCGGACTACT
EcoRI
|
2461 atgctcatccggaattccgtatggcaatgaaagacggtgagctggtgatatgggatagtg 2520
TACGAGTAGGCCTTAAGGCATACCGTTACTTTCTGCCACTCGACCACTATACCCTATCAC
2521 ttcacccttgttacaccgttttccatgagcaaactgaaacgttttcatcgctctggagtg 2580
AAGTGGGAACAATGTGGCAAAAGGTACTCGTTTGACTTTGCAAAAGTAGCGAGACCTCAC
2581 aataccacgacgatttccggcagtttctacacatatattcgcaagatgtggcgtgttacg 2640
TTATGGTGCTGCTAAAGGCCGTCAAAGATGTGTATATAAGCGTTCTACACCGCACAATGC
2641 gtgaaaacctggcctatttccctaaagggtttattgagaatatgtttttcgtctcagcca 2700
CACTTTTGGACCGGATAAAGGGATTTCCCAAATAACTCTTATACAAAAAGCAGAGTCGGT
2701 atccctgggtgagtttcaccagttttgatttaaacgtggccaatatggacaacttcttcg 2760
TAGGGACCCACTCAAAGTGGTCAAAACTAAATTTGCACCGGTTATACCTGTTGAAGAAGC
2761 cccccgttttcaccatgggcaaatattatacgcaaggcgacaaggtgctgatgccgctgg 2820
GGGGGCAAAAGTGGTACCCGTTTATAATATGCGTTCCGCTGTTCCACGACTACGGCGACC
2821 cgattcaggttcatcatgccgtttgtgatggcttccatgtcggcagaatgcttaatgaat 2880
GCTAAGTCCAAGTAGTACGGCAAACACTACCGAAGGTACAGCCGTCTTACGAATTACTTA
2881 tacaacagtactgcgatgagtggcagggcggggcgtaaagatctggatccggcttactaa 2940
ATGTTGTCATGACGCTACTCACCGTCCCGCCCCGCATTTCTAGACCTAGGCCGAATGATT
2941 aagccagataacagtatgcgtatttgcgcgctgatttttgcggtataagaatatatactg 3000
TTCGGTCTATTGTCATACGCATAAACGCGCGACTAAAAACGCCATATTCTTATATATGAC
3001 atatgtatacccgaagtatgtcaaaaagaggtatgctatgaagcagcgtattacagtgac 3060
TATACATATGGGCTTCATACAGTTTTTCTCCATACGATACTTCGTCGCATAATGTCACTG
3061 agttgacagcgacagctatcagttgctcaaggcatatatgatgtcaatatctccggtctg 3120
TCAACTGTCGCTGTCGATAGTCAACGAGTTCCGTATATACTACAGTTATAGAGGCCAGAC
3121 gtaagcacaaccatgcagaatgaagcccgtcgtctgcgtgccgaacgctggaaagcggaa 3180
CATTCGTGTTGGTACGTCTTACTTCGGGCAGCAGACGCACGGCTTGCGACCTTTCGCCTT
3181 aatcaggaagggatggctgaggtcgcccggtttattgaaatgaacggctcttttgctgac 3240
TTAGTCCTTCCCTACCGACTCCAGCGGGCCAAATAACTTTACTTGCCGAGAAAACGACTG
ccdB other(3260,3565)>>>
|
3241 gagaacaggggctggtgaaatgcagtttaaggtttacacctataaaagagagagccgtta 3300
CTCTTGTCCCCGACCACTTTACGTCAAATTCCAAATGTGGATATTTTCTCTCTCGGCAAT
3301 tcgtctgtttgtggatgtacagagtgatattattgacacgcccgggcgacggatggtgat 3360
AGCAGACAAACACCTACATGTCTCACTATAATAACTGTGCGGGCCCGCTGCCTACCACTA
3361 ccccctggccagtgcacgtctgctgtcagataaagtctcccgtgaactttacccggtggt 3420
GGGGGACCGGTCACGTGCAGACGACAGTCTATTTCAGAGGGCACTTGAAATGGGCCACCA
3421 gcatatcggggatgaaagctggcgcatgatgaccaccgatatggccagtgtgccggtctc 3480
CGTATAGCCCCTACTTTCGACCGCGTACTACTGGTGGCTATACCGGTCACACGGCCAGAG
3481 cgttatcggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccat 3540
GCAATAGCCCCTTCTTCACCGACTAGAGTCGGTGGCGCTTTTACTGTAGTTTTTGCGGTA
3541 taacctgatgttctggggaatataaatgtcaggctcccttatacacagccagtctgcagg 3600
ATTGGACTACAAGACCCCTTATATTTACAGTCCGAGGGAATATGTGTCGGTCAGACGTCC
attR1 other(3606,3725)<<<
|
attR2 other(3606,3706)>>>
|
3601 tcgaccatagtgactggatatgttgtgttttacagtattatgtagtctgttttttatgca 3660
AGCTGGTATCACTGACCTATACAACACAAAATGTCATAATACATCAGACAAAAAATACGT
3661 aaatctaatttaatatattgatatttatatcattttacgtttctcgttcagctttcttgt 3720
TTTAGATTAAATTATATAACTATAAATATAGTAAAATGCAAAGAGCAAGTCGAAAGAACA
XhoI hGH_PA term(3758,4234)>>>
| |
3721 acaaagtggttgatatctgactcgagccaatCTTAAGgggtggcatccctgtgacccctc 3780
TGTTTCACCAACTATAGACTGAGCTCGGTTAGAATTCCCCACCGTAGGGACACTGGGGAG
3781 cccagtgcctctcctggccctggaagttgccactccagtgcccaccagccttgtcctaat 3840
GGGTCACGGAGAGGACCGGGACCTTCAACGGTGAGGTCACGGGTGGTCGGAACAGGATTA
3841 aaaattaagttgcatcattttgtctgactaggtgtccttctataatattatggggtggag 3900
TTTTAATTCAACGTAGTAAAACAGACTGATCCACAGGAAGATATTATAATACCCCACCTC
3901 gggggtggtatggagcaaggggcaagttgggaagacaacctgtagggcctgcggggtcta 3960
CCCCCACCATACCTCGTTCCCCGTTCAACCCTTCTGTTGGACATCCCGGACGCCCCAGAT
3961 ttgggaaccaagctggagtgcagtggcacaatcttggctcactgcaatctccgcctcctg 4020
AACCCTTGGTTCGACCTCACGTCACCGTGTTAGAACCGAGTGACGTTAGAGGCGGAGGAC
SphI
|
4021 ggttcaagcgattctcctgcctcagcctcccgagttgttgggattccaggcatgcatgac 4080
CCAAGTTCGCTAAGAGGACGGAGTCGGAGGGCTCAACAACCCTAAGGTCCGTACGTACTG
4081 caggctcagctaatttttgtttttttggtagagacggggtttcaccatattggccaggct 4140
GTCCGAGTCGATTAAAAACAAAAAAACCATCTCTGCCCCAAAGTGGTATAACCGGTCCGA
4141 ggtctccaactcctaatctcaggtgatctacccaccttggcctcccaaattgctgggatt 4200
CCAGAGGTTGAGGATTAGAGTCCACTAGATGGGTGGAACCGGAGGGTTTAACGACCCTAA
4201 acaggcgtgaaccactgctcccttccctgtccttcacgtgcggaccgagcggccgcagga 4260
TGTCCGCACTTGGTGACGAGGGAAGGGACAGGAAGTGCACGCCTGGCTCGCCGGCGTCCT
4261 acccctagtgatggagttggccactccctctctgcgcgctcgctcgctcactgaggccgg 4320
TGGGGATCACTACCTCAACCGGTGAGGGAGAGACGCGCGAGCGAGCGAGTGACTCCGGCC
4321 gcgaccaaaggtcgcccgacgcccgggctttgcccgggcggcctcagtgagcgagcgagc 4380
CGCTGGTTTCCAGCGGGCTGCGGGCCCGAAACGGGCCCGCCGGAGTCACTCGCTCGCTCG
4381 gcgcagctgcctgcaggggcgcctgatgcggtattttctccttacgcatctgtgcggtat 4440
CGCGTCGACGGACGTCCCCGCGGACTACGCCATAAAAGAGGAATGCGTAGACACGCCATA
f1 origin(4489,4795)>>>
|
4441 ttcacaccgcatacgtcaaagcaaccatagtacgcgccctgtagcggcgcattaagcgcg 4500
AAGTGTGGCGTATGCAGTTTCGTTGGTATCATGCGCGGGACATCGCCGCGTAATTCGCGC
4501 gcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccttagcgcccgct 4560
CGCCCACACCACCAATGCGCGTCGCACTGGCGATGTGAACGGTCGCGGAATCGCGGGCGA
4561 cctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctcta 4620
GGAAAGCGAAAGAAGGGAAGGAAAGAGCGGTGCAAGCGGCCGAAAGGGGCAGTTCGAGAT
4621 aatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaa 4680
TTAGCCCCCGAGGGAAATCCCAAGGCTAAATCACGAAATGCCGTGGAGCTGGGGTTTTTT
4681 cttgatttgggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccct 4740
GAACTAAACCCACTACCAAGTGCATCACCCGGTAGCGGGACTATCTGCCAAAAAGCGGGA
4741 ttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactc 4800
AACTGCAACCTCAGGTGCAAGAAATTATCACCTGAGAACAAGGTTTGACCTTGTTGTGAG
4801 aactctatctcgggctattcttttgatttataagggattttgccgatttcggtctattgg 4860
TTGAGATAGAGCCCGATAAGAAAACTAAATATTCCCTAAAACGGCTAAAGCCAGATAACC
4861 ttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgttt 4920
AATTTTTTACTCGACTAAATTGTTTTTAAATTGCGCTTAAAATTGTTTTATAATTGCAAA
4921 acaattttatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagccc 4980
TGTTAAAATACCACGTGAGAGTCATGTTAGACGAGACTACGGCGTATCAATTCGGTCGGG
4981 cgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgct 5040
GCTGTGGGCGGTTGTGGGCGACTGCGCGGGACTGCCCGAACAGACGAGGGCCGTAGGCGA
5041 tacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatca 5100
ATGTCTGTTCGACACTGGCAGAGGCCCTCGACGTACACAGTCTCCAAAAGTGGCAGTAGT
5101 ccgaaacgcgcgagccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacg 5160
GGCTTTGCGCGCTCGGCCCTCGACGTACACAGTCTCCAAAAGTGGCAGTAGTGGCTTTGC
5161 cgcgagacgaaagggcctcgtgatacgcctatttttataggttaatgtcatgataataat 5220
GCGCTCTGCTTTCCCGGAGCACTATGCGGATAAAAATATCCAATTACAGTACTATTATTA
5221 ggtttcttagacgtcaggtggcacttttcggggaaatgtgcgcggaacccctatttgttt 5280
CCAAAGAATCTGCAGTCCACCGTGAAAAGCCCCTTTACACGCGCCTTGGGGATAAACAAA
amp prom(5296,5324)>>>
|
5281 atttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgct 5340
TAAAAAGATTTATGTAAGTTTATACATAGGCGAGTACTCTGTTATTGGGACTATTTACGA
ORF_1 rf(2)(5366,6226)>>>
|
amp marker(5366,6226)>>>
|
5341 tcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcc 5400
AGTTATTATAACTTTTTCCTTCTCATACTCATAAGTTGTAAAGGCACAGCGGGAATAAGG
5401 cttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaa 5460
GAAAAAACGCCGTAAAACGGAAGGACAAAAACGAGTGGGTCTTTGCGACCACTTTCATTT
5461 agatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcgg 5520
TCTACGACTTCTAGTCAACCCACGTGCTCACCCAATGTAGCTTGACCTAGAGTTGTCGCC
5521 taagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagt 5580
ATTCTAGGAACTCTCAAAAGCGGGGCTTCTTGCAAAAGGTTACTACTCGTGAAAATTTCA
5581 tctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccg 5640
AGACGATACACCGCGCCATAATAGGGCATAACTGCGGCCCGTTCTCGTTGAGCCAGCGGC
5641 catacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttac 5700
GTATGTGATAAGAGTCTTACTGAACCAACTCATGAGTGGTCAGTGTCTTTTCGTAGAATG
5701 ggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgc 5760
CCTACCGTACTGTCATTCTCTTAATACGTCACGACGGTATTGGTACTCACTATTGTGACG
5761 ggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaa 5820
CCGGTTGAATGAAGACTGTTGCTAGCCTCCTGGCTTCCTCGATTGGCGAAAAAACGTGTT
5821 catgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccatacc 5880
GTACCCCCTAGTACATTGAGCGGAACTAGCAACCCTTGGCCTCGACTTACTTCGGTATGG
5881 aaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactatt 5940
TTTGCTGCTCGCACTGTGGTGCTACGGACATCGTTACCGTTGTTGCAACGCGTTTGATAA
5941 aactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcgga 6000
TTGACCGCTTGATGAATGAGATCGAAGGGCCGTTGTTAATTATCTGACCTACCTCCGCCT
6001 taaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataa 6060
ATTTCAACGTCCTGGTGAAGACGCGAGCCGGGAAGGCCGACCGACCAAATAACGACTATT
6061 atctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaa 6120
TAGACCTCGGCCACTCGCACCCAGAGCGCCATAGTAACGTCGTGACCCCGGTCTACCATT
6121 gccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaa 6180
CGGGAGGGCATAGCATCAATAGATGTGCTGCCCCTCAGTCCGTTGATACCTACTTGCTTT
6181 tagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagt 6240
ATCTGTCTAGCGACTCTATCCACGGAGTGACTAATTCGTAACCATTGACAGTCTGGTTCA
6241 ttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggt 6300
AATGAGTATATATGAAATCTAACTAAATTTTGAAGTAAAAATTAAATTTTCCTAGATCCA
6301 gaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactg 6360
CTTCTAGGAAAAACTATTAGAGTACTGGTTTTAGGGAATTGCACTCAAAAGCAAGGTGAC
pBR322 origin(6381,7000)>>>
|
6361 agcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgt 6420
TCGCAGTCTGGGGCATCTTTTCTAGTTTCCTAGAAGAACTCTAGGAAAAAAAGACGCGCA
6421 aatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatca 6480
TTAGACGACGAACGTTTGTTTTTTTGGTGGCGATGGTCGCCACCAAACAAACGGCCTAGT
6481 agagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatac 6540
TCTCGATGGTTGAGAAAAAGGCTTCCATTGACCGAAGTCGTCTCGCGTCTATGGTTTATG
6541 tgttcttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctac 6600
ACAAGAAGATCACATCGGCATCAATCCGGTGGTGAAGTTCTTGAGACATCGTGGCGGATG
6601 atacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtct 6660
TATGGAGCGAGACGATTAGGACAATGGTCACCGACGACGGTCACCGCTATTCAGCACAGA
6661 taccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggg 6720
ATGGCCCAACCTGAGTTCTGCTATCAATGGCCTATTCCGCGTCGCCAGCCCGACTTGCCC
6721 gggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctaca 6780
CCCAAGCACGTGTGTCGGGTCGAACCTCGCTTGCTGGATGTGGCTTGACTCTATGGATGT
6781 gcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggt 6840
CGCACTCGATACTCTTTCGCGGTGCGAAGGGCTTCCCTCTTTCCGCCTGTCCATAGGCCA
6841 aagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggta 6900
TTCGCCGTCCCAGCCTTGTCCTCTCGCGTGCTCCCTCGAAGGTCCCCCTTTGCGGACCAT
6901 tctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctc 6960
AGAAATATCAGGACAGCCCAAAGCGGTGGAGACTGAACTCGCAGCTAAAAACACTACGAG
6961 gtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggc 7020
CAGTCCCCCCGCCTCGGATACCTTTTTGCGGTCGTTGCGCCGGAAAAATGCCAAGGACCG
7021 cttttgctggccttttgctcacatgt 7046
GAAAACGACCGGAAAACGAGTGTACA
LOCUS dna 7046 bp
FEATURES Location/Qualifiers
misc_binding 413..418
/dbxref="REBASE:NdeI"
Promoter 545..822
/gene="cBA prom"
ORF 962..1693
/sequence="ORF_3 rf(5)"
misc_binding 1019..1024
/dbxref="REBASE:ApaI"
misc_binding 1783..1788
/dbxref="REBASE:XbaI"
misc_binding 1892..1897
/dbxref="REBASE:SacI"
Regulatory_Seq 1973..2012
/gene="tetO reg"
Other Gene 2026..2150
/gene="attR1 other"
Other Gene 2050..2150
/gene="attR2 other"
Marker 2259..2918
/gene="CAT marker"
ORF 2259..2918
/sequence="ORF_2 rf(3)"
misc_binding 2472..2477
/dbxref="REBASE:EcoRI"
Other Gene 3260..3565
/gene="ccdB other"
Other Gene 3606..3725
/gene="attR1 other"
Other Gene 3606..3706
/gene="attR2 other"
misc_binding 3741..3746
/dbxref="REBASE:XhoI"
Terminator 3758..4234
/gene="hGH_PA term"
misc_binding 4070..4075
/dbxref="REBASE:SphI"
Rep_Origin 4489..4795
/gene="f1 origin"
Promoter 5296..5324
/gene="amp prom"
Marker 5366..6226
/gene="amp marker"
ORF 5366..6226
/sequence="ORF_1 rf(2)"
Rep_Origin 6381..7000
/gene="pBR322 origin"
BASE COUNT 1540 a 1793 c 1976 g 1737 t 0 others