Custom Retrovirus Services

abm’s custom retroviruses are a highly-efficient method to achieve stable gene expression in a broad range of dividing mammalian cells. Simply send us your gene, or purchase an ORF vector from our extensive library of human, mouse, or rat genes, and we will do the rest! Our packaged retroviruses are available with your choice of no tag, HA tag, or GFP reporter.

KEY FEATURES OF abm's CUSTOM RETROVIRUS SERVICE
Promoter	CMV promoter for strong gene expression in most cellular systems
Tags Available	No tag HA tag for detection or protein purification GFP reporter
Safety	Replication-incompetent retrovirus with minimal relation to the wild-type Moloney Murine Leukemia Virus (MoMuLV)

Additional Resources:

"abm has always been very helpful with technical assistance in any of our questions and concerns. I also consider the price for the services provided as very competitive, as other companies charge a multiple of abm's price."

Dr. Cameron Moshfegh, ETH Zurich

Service Details

Custom Retroviral Vectors

Service Name	Description	Unit	CAT. NO.	PRICE
Custom Retroviral Vector using ORF Library with No Tag	Any human, mouse, or rat gene from our extensive ORF library, available in a retroviral vector with no tag. Corresponding ORF vector must be ordered with this service. Maximum insert size that can be packaged is 4.5 kb.	1.0 μg	C362	$150.00
Custom Retroviral Vector using ORF Library with HA Tag	Any human, mouse, or rat gene from our extensive ORF library, available in a retroviral vector with a C-term HA tag. Corresponding ORF vector must be ordered with this service. Maximum insert size that can be packaged is 4.5 kb.	1.0 μg	C363	$300.00
Custom Retroviral Vector using ORF Library with GFP Reporter	Any human, mouse, or rat gene from our extensive ORF library, available in a retroviral vector with a GFP reporter. Corresponding ORF vector must be ordered with this service. Maximum insert size that can be packaged is 3.2 kb.	1.0 μg	C364	$300.00
Custom Retroviral Vector	Custom retrovirus plasmid subcloning. Provide your DNA and we’ll subclone it into a retroviral vector.	1 Service	C057	$550.00
Gene Synthesis	For any sequence up to 4.5 kb in size. Genes are fully-sequenced. Must be ordered with Custom Retroviral Vector (C362, C363 or C364). * Minimum charge of $99.00	1.0 μg	C098	$0.18/bp USD

Additional cloning services are available.

Custom Retroviruses

Service Name		Quant. DNA Provided	CAT. NO.	Quant	PRICE
Custom Retrovirus	Enough DNA Provided	≥60 μg@1μg/μl	C510	1 ml at 10⁷ IU/ml	$600
	DNA Amplification Required	<60 μg	C517	1 ml at 10⁷ IU/ml	$700

Please Note: For customer-supplied vectors, the customer must indicate prior to the service if any reporter expression will be expected or not. The expected reporter expression, e.g. GFP, should not require induction of expression. Due to differences in excitation/emission wavelengths for different fluorophores, we may not be able to provide infection images for fluorescent reporter expression other than standard GFP/RFP/mCherry.

Retrovirus Controls

Product	Unit	CAT. NO.	PRICE
pRetroG-CMV-blank Vector	1.0 μg	RV001	$135.00
pRetroG-CMV-GFP-HA Vector	1.0 μg	RV002	$135.00
pRetroG-CMV-GFP-stop Vector	1.0 μg	RV003	$135.00
pRetroG-CMV-GFP-IRES-GFP Vector	1.0 μg	RV004	$135.00
pRetroG-CMV-RFP-stop Vector	1.0 μg	RV005	$135.00
Retro-CMV Blank Retrovirus	1 ml of 10⁷ IU/ml	RVP001	$195.00
Retro-CMV-GFP-HA Retrovirus	1 ml of 10⁷ IU/ml	RVP002	$195.00
Retro-CMV-GFP Retrovirus	1 ml of 10⁷ IU/ml	RVP003	$195.00
Retro-CMV-GFP-IRES-GFP Retrovirus	1 ml of 10⁷ IU/ml	RVP004	$195.00
Retro-CMV-RFP Retrovirus	1 ml of 10⁷ IU/ml	RVP005	$195.00

Retrovirus Vectors (click for details):

Additional Info

Documents

FAQs

How long does it take to produce virus?

This will be dependent on a number of factors, including the plasmid you would like to package. Please send all inquiries to technical@abmgood.com for a prompt response on the estimated lead time.

How do you determine titer?

Via qPCR

What is the packaging capacity for Retrovirus?

The maximum insert size is <8 kb between the 5'LTR to 3'LTR.

What is the minimum amount of DNA needed for subcloning into a retroviral vector (C517)?

The minimum amount of DNA we require is 60μg(at 1μg/μl) for subcloning into a retroviral vector.

Citations

01	Thomas, A. M. et al. "Polysaccharide-modified scaffolds for controlled lentivirus delivery in vitro and after spinal cord injury." J Control Release. 3:421-429 (2013). DOI: 10.1016/j.jconrel.2013.06.013 PubMed: 23791981