pRetroG-CMV-IRES-GFP
| Vector Name | pRetroG-CMV-IRES-GFP |
| VectorType | Retroviral Plasmid |
| Antibiotic Information | Bacterial: Ampicillin Mammalian: Puromycin |
| Sequencing Primers | CMV sequencing primer 5`---CGC AAA TGG GCG GTA GGC GTG---3` |
| Additional Information | The gene expression is driven by a CMV promoter, with a C-terminal IRES-GFP reporter Original Vector: 9405bp Vector without Insert Cassette: 7700bp |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 tttgaaagac cccacccgta ggtggcaagc tagcttaagt aacgccactt tgcaaggcat 61 ggaaaaatac ataactgaga atagaaaagt tcagatcaag gtcaggaaca aagaaacagc 121 tgaataccaa acaggatatc tgtggtaagc ggttcctgcc ccggctcagg gccaagaaca 181 gatgagacag ctgagtgatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg 241 ctcggggcca agaacagatg gtccccagat gcggtccagc cctcagcagt ttctagtgaa 301 tcatcagatg tttccagggt gccccaagga cctgaaaatg accctgtacc ttatttgaac 361 taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc cgctctccga gctcaataaa 421 agagcccaca acccctcact cggcgcgcca gtcttccgat agactgcgtc gcccgggtac 481 ccgtattccc aataaagcct cttgctgttt gcatccgaat cgtggtctcg ctgttccttg 541 ggagggtctc ctctgagtga ttgactaccc acgacggggg tctttcattt gggggctcgt 601 ccgggatttg gagacccctg cccagggacc accgacccac caccgggagg taagctggcc 661 agcaacttat ctgtgtctgt ccgattgtct agtgtctatg tttgatgtta tgcgcctgcg 721 tctgtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa ctgacgagtt 781 ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc gtttttgtgg 841 cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg tggttctggt 901 aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt cggtttggaa 961 ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct 1021 gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc ccttaagttt 1081 gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa 1141 gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg tcggatggcc 1201 gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg tcttttcacc 1261 tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag ccttggcttt 1321 tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc ctcttcctcc 1381 atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta 1441 tccagccctc actccttctc taggcgccgg aattgatccc ccgggctgca ggtcggccgc 1501 cacgaccggt gccgccacca tcccctgacc cacgcccctg acccctcaca aggagacgac 1561 cttccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtcccccggg 1621 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgacc 1681 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc 1741 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc 1801 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga 1861 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca 1921 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc 1981 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct 2041 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca 2101 ccgccgacgt cgagtgcccg aaggaccgcg cgacctggtg catgacccgc aagcccggtg 2161 cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga ccccatggct 2221 ccgaccgaag ccgacccggg cggccccgcc gaccccgcac ccgcccccga ggcccaccga 2281 ctctagtaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc 2341 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg 2401 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa 2461 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca 2521 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac 2581 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc 2641 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga 2701 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg 2761 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta 2821 cggtgggagg tctatataag cagagctggt ttagtgaacc gtcagatccg ctagcgctac 2881 cggactcaga tctaagcttc ctagcgatat caacaagttt gtacaaaaaa gctgaacgag 2941 aaacgtaaaa tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta 3001 cataatactg taaaacacaa catatccagt cactatggcg gccgcattag gcaccccagg 3061 ctttacactt tatgcttccg gctcgtataa tgtgtggatt ttgagttagg atccgtcgag 3121 attttcagga gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga 3181 tatatcccaa tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac 3241 ctataaccag accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa 3301 gcacaagttt tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga 3361 attccgtatg gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta 3421 caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga 3481 tttccggcag tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc 3541 ctatttccct aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag 3601 tttcaccagt tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac 3661 catgggcaaa tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca 3721 tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg 3781 cgatgagtgg cagggcgggg cgtaaagatc tggatccggc ttactaaaag ccagataaca 3841 gtatgcgtat ttgcgcgctg atttttgcgg tataagaata tatactgata tgtatacccg 3901 aagtatgtca aaaagaggta tgctatgaag cagcgtatta cagtgacagt tgacagcgac 3961 agctatcagt tgctcaaggc atatatgatg tcaatatctc cggtctggta agcacaacca 4021 tgcagaatga agcccgtcgt ctgcgtgccg aacgctggaa agcggaaaat caggaaggga 4081 tggctgaggt cgcccggttt attgaaatga acggctcttt tgctgacgag aacaggggct 4141 ggtgaaatgc agtttaaggt ttacacctat aaaagagaga gccgttatcg tctgtttgtg 4201 gatgtacaga gtgatattat tgacacgccc gggcgacgga tggtgatccc cctggccagt 4261 gcacgtctgc tgtcagataa agtctcccgt gaactttacc cggtggtgca tatcggggat 4321 gaaagctggc gcatgatgac caccgatatg gccagtgtgc cggtctccgt tatcggggaa 4381 gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc 4441 tggggaatat aaatgtcagg ctcccttata cacagccagt ctgcaggtcg accatagtga 4501 ctggatatgt tgtgttttac agtattatgt agtctgtttt ttatgcaaaa tctaatttaa 4561 tatattgata tttatatcat tttacgtttc tcgttcagct ttcttgtaca aagtggttga 4621 tatctgactc gaggttaacg aattccctct ccctcccccc cccctaacgt tactggccga 4681 agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg 4741 tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg 4801 ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt 4861 cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac 4921 cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca 4981 aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg 5041 ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg 5101 ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa 5161 cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg atgataatat 5221 ggccacaacc atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt 5281 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga 5341 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc 5401 ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga 5461 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg 5521 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg 5581 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat 5641 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa 5701 gcagaagaac ggcatcaagg cgaacttcaa gatccgccac aacatcgagg acggcagcgt 5761 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc 5821 cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga 5881 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct 5941 gtacaagtaa tgaattaatt aagaattatc accgcttcta ttcagccagt aaggcctgtc 6001 tttaatggcc tccggcatga gacactttct agagggcccg tttaaacgaa ttcgttaggc 6061 cattaaggcc tgtcgacaag cggccgcctc ggccaaacat cgataaaata aaagatttta 6121 tttagtctcc agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg caagctagct 6181 taagtaacgc cattttgcaa ggcatggaaa aatacataac tgagaataga gaagttcaga 6241 tcaaggtcag gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc 6301 agttcctgcc ccggctcagg gccaagaaca gatggaacag ctgaatatgg gccaaacagg 6361 atatctgtgg taagcagttc ctgccccggc tcagggccaa gaacagatgg tccccagatg 6421 cggtccagcc ctcagcagtt tctagagaac catcagatgt ttccagggtg ccccaaggac 6481 ctgaaatgac cctgtgcctt atttgaacta accaatcagt tcgcttctcg cttctgttcg 6541 cgcgcttctg ctccccgagc tcaataaaag agcccacaac ccctcactcg gggcgccagt 6601 cctccgattg actgagtcgc ccgggtaccc gtgtatccaa taaaccctct tgcagttgca 6661 tccgacttgt ggtctcgctg ttccttggga gggtctcctc tgagtgattg actacccgtc 6721 agcgggggtc tttcatttgg gggctcgtcc gggatcggga gacccctgcc cagggaccac 6781 cgacccacca ccgggaggta agctggctgc ctcgcgcgtt tcggtgatga cggtgaaaac 6841 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc 6901 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc 6961 cagtcacgta gcgatagcgg agtgtactga taacttcgta taatgtatgc tatacgaagt 7021 tattaggtct gaagaggagt ttacgtccag ccaagctagg gccgcgatcc ggaaccctta 7081 atataacttc gtataatgta tgctatacga agttatcagt actggcttaa ctatgcggca 7141 tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta 7201 aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg 7261 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca 7321 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac 7381 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac 7441 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg 7501 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac 7561 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat 7621 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag 7681 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac 7741 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt 7801 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt 7861 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc 7921 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga 7981 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac 8041 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc 8101 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct 8161 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca 8221 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct 8281 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca 8341 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc 8401 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg 8461 cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct 8521 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa 8581 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta 8641 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc 8701 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg 8761 agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa 8821 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg 8881 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc 8941 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg 9001 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat 9061 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata 9121 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc 9181 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtcttca agaattgcta 9241 gcaattgcta gcaattgcta gcaattcata ccagatcacc gaaaactgtc ctccaaatgt 9301 gtccccctca cactcccaaa ttcgcgggct tctgcctctt agaccactct accctattcc 9361 ccacactcac cggagccaaa gccgcggccc ttccgtttct ttgct
LOCUS dna 9405 bp FEATURES Location/Qualifiers Other Gene 659..1468 /gene="psi_plus_pack other" ORF 1449..2216 /sequence="ORF_4 rf(5)" Marker 1566..2165 /gene="puro marker" Promoter 2787..2868 /gene="CMV prom" Other Gene 2913..3037 /gene="attR1 other" Other Gene 2937..3037 /gene="attR2 other" Marker 3146..3805 /gene="CAT marker" ORF 3146..3805 /sequence="ORF_1 rf(2)" Other Gene 4147..4452 /gene="ccdB other" Other Gene 4493..4612 /gene="attR1 other" Other Gene 4493..4593 /gene="attR2 other" misc_binding 4628..4633 /dbxref="REBASE:XhoI" misc_binding 4634..4639 /dbxref="REBASE:HpaI" Regulatory_Seq 4645..5227 /gene="IRES reg" Regulatory_Seq 4719..5219 /gene="ires_emcv reg" ORF 5219..5950 /sequence="ORF_2 rf(2)" Reporter 5231..5947 /gene="EGFP reporter" misc_binding 6099..6104 /dbxref="REBASE:ClaI" Other Gene 6186..6737 /gene="5_LTR2 other" Other Gene 6990..7023 /gene="loxP other" Other Gene 7083..7116 /gene="loxP other" Rep_Origin 7392..8011 /gene="pBR322 origin" Marker 8166..9026 /gene="amp marker" ORF 8166..9026 /sequence="ORF_3 rf(5)" Promoter 9068..9096 /gene="amp prom" BASE COUNT 2269 a 2580 c 2378 g 2178 t 0 others ORIGIN 1 tttgaaagac cccacccgta ggtggcaagc tagcttaagt aacgccactt tgcaaggcat 61 ggaaaaatac ataactgaga atagaaaagt tcagatcaag gtcaggaaca aagaaacagc 121 tgaataccaa acaggatatc tgtggtaagc ggttcctgcc ccggctcagg gccaagaaca 181 gatgagacag ctgagtgatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg 241 ctcggggcca agaacagatg gtccccagat gcggtccagc cctcagcagt ttctagtgaa 301 tcatcagatg tttccagggt gccccaagga cctgaaaatg accctgtacc ttatttgaac 361 taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc cgctctccga gctcaataaa 421 agagcccaca acccctcact cggcgcgcca gtcttccgat agactgcgtc gcccgggtac 481 ccgtattccc aataaagcct cttgctgttt gcatccgaat cgtggtctcg ctgttccttg 541 ggagggtctc ctctgagtga ttgactaccc acgacggggg tctttcattt gggggctcgt 601 ccgggatttg gagacccctg cccagggacc accgacccac caccgggagg taagctggcc 661 agcaacttat ctgtgtctgt ccgattgtct agtgtctatg tttgatgtta tgcgcctgcg 721 tctgtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa ctgacgagtt 781 ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc gtttttgtgg 841 cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg tggttctggt 901 aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt cggtttggaa 961 ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct 1021 gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc ccttaagttt 1081 gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa 1141 gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg tcggatggcc 1201 gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg tcttttcacc 1261 tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag ccttggcttt 1321 tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc ctcttcctcc 1381 atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta 1441 tccagccctc actccttctc taggcgccgg aattgatccc ccgggctgca ggtcggccgc 1501 cacgaccggt gccgccacca tcccctgacc cacgcccctg acccctcaca aggagacgac 1561 cttccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtcccccggg 1621 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgacc 1681 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc 1741 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc 1801 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga 1861 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca 1921 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc 1981 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct 2041 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca 2101 ccgccgacgt cgagtgcccg aaggaccgcg cgacctggtg catgacccgc aagcccggtg 2161 cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga ccccatggct 2221 ccgaccgaag ccgacccggg cggccccgcc gaccccgcac ccgcccccga ggcccaccga 2281 ctctagtaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc 2341 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg 2401 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa 2461 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca 2521 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac 2581 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc 2641 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga 2701 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg 2761 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta 2821 cggtgggagg tctatataag cagagctggt ttagtgaacc gtcagatccg ctagcgctac 2881 cggactcaga tctaagcttc ctagcgatat caacaagttt gtacaaaaaa gctgaacgag 2941 aaacgtaaaa tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta 3001 cataatactg taaaacacaa catatccagt cactatggcg gccgcattag gcaccccagg 3061 ctttacactt tatgcttccg gctcgtataa tgtgtggatt ttgagttagg atccgtcgag 3121 attttcagga gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga 3181 tatatcccaa tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac 3241 ctataaccag accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa 3301 gcacaagttt tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga 3361 attccgtatg gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta 3421 caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga 3481 tttccggcag tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc 3541 ctatttccct aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag 3601 tttcaccagt tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac 3661 catgggcaaa tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca 3721 tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg 3781 cgatgagtgg cagggcgggg cgtaaagatc tggatccggc ttactaaaag ccagataaca 3841 gtatgcgtat ttgcgcgctg atttttgcgg tataagaata tatactgata tgtatacccg 3901 aagtatgtca aaaagaggta tgctatgaag cagcgtatta cagtgacagt tgacagcgac 3961 agctatcagt tgctcaaggc atatatgatg tcaatatctc cggtctggta agcacaacca 4021 tgcagaatga agcccgtcgt ctgcgtgccg aacgctggaa agcggaaaat caggaaggga 4081 tggctgaggt cgcccggttt attgaaatga acggctcttt tgctgacgag aacaggggct 4141 ggtgaaatgc agtttaaggt ttacacctat aaaagagaga gccgttatcg tctgtttgtg 4201 gatgtacaga gtgatattat tgacacgccc gggcgacgga tggtgatccc cctggccagt 4261 gcacgtctgc tgtcagataa agtctcccgt gaactttacc cggtggtgca tatcggggat 4321 gaaagctggc gcatgatgac caccgatatg gccagtgtgc cggtctccgt tatcggggaa 4381 gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc 4441 tggggaatat aaatgtcagg ctcccttata cacagccagt ctgcaggtcg accatagtga 4501 ctggatatgt tgtgttttac agtattatgt agtctgtttt ttatgcaaaa tctaatttaa 4561 tatattgata tttatatcat tttacgtttc tcgttcagct ttcttgtaca aagtggttga 4621 tatctgactc gaggttaacg aattccctct ccctcccccc cccctaacgt tactggccga 4681 agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg 4741 tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg 4801 ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt 4861 cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac 4921 cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca 4981 aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg 5041 ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg 5101 ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa 5161 cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg atgataatat 5221 ggccacaacc atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt 5281 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga 5341 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc 5401 ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga 5461 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg 5521 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg 5581 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat 5641 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa 5701 gcagaagaac ggcatcaagg cgaacttcaa gatccgccac aacatcgagg acggcagcgt 5761 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc 5821 cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga 5881 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct 5941 gtacaagtaa tgaattaatt aagaattatc accgcttcta ttcagccagt aaggcctgtc 6001 tttaatggcc tccggcatga gacactttct agagggcccg tttaaacgaa ttcgttaggc 6061 cattaaggcc tgtcgacaag cggccgcctc ggccaaacat cgataaaata aaagatttta 6121 tttagtctcc agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg caagctagct 6181 taagtaacgc cattttgcaa ggcatggaaa aatacataac tgagaataga gaagttcaga 6241 tcaaggtcag gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc 6301 agttcctgcc ccggctcagg gccaagaaca gatggaacag ctgaatatgg gccaaacagg 6361 atatctgtgg taagcagttc ctgccccggc tcagggccaa gaacagatgg tccccagatg 6421 cggtccagcc ctcagcagtt tctagagaac catcagatgt ttccagggtg ccccaaggac 6481 ctgaaatgac cctgtgcctt atttgaacta accaatcagt tcgcttctcg cttctgttcg 6541 cgcgcttctg ctccccgagc tcaataaaag agcccacaac ccctcactcg gggcgccagt 6601 cctccgattg actgagtcgc ccgggtaccc gtgtatccaa taaaccctct tgcagttgca 6661 tccgacttgt ggtctcgctg ttccttggga gggtctcctc tgagtgattg actacccgtc 6721 agcgggggtc tttcatttgg gggctcgtcc gggatcggga gacccctgcc cagggaccac 6781 cgacccacca ccgggaggta agctggctgc ctcgcgcgtt tcggtgatga cggtgaaaac 6841 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc 6901 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc 6961 cagtcacgta gcgatagcgg agtgtactga taacttcgta taatgtatgc tatacgaagt 7021 tattaggtct gaagaggagt ttacgtccag ccaagctagg gccgcgatcc ggaaccctta 7081 atataacttc gtataatgta tgctatacga agttatcagt actggcttaa ctatgcggca 7141 tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta 7201 aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg 7261 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca 7321 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac 7381 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac 7441 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg 7501 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac 7561 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat 7621 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag 7681 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac 7741 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt 7801 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt 7861 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc 7921 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga 7981 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac 8041 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc 8101 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct 8161 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca 8221 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct 8281 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca 8341 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc 8401 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg 8461 cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct 8521 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa 8581 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta 8641 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc 8701 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg 8761 agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa 8821 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg 8881 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc 8941 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg 9001 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat 9061 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata 9121 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc 9181 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtcttca agaattgcta 9241 gcaattgcta gcaattgcta gcaattcata ccagatcacc gaaaactgtc ctccaaatgt 9301 gtccccctca cactcccaaa ttcgcgggct tctgcctctt agaccactct accctattcc 9361 ccacactcac cggagccaaa gccgcggccc ttccgtttct ttgct //
***Note: The text map shown below is representative of the parental
vector only and does not include the gene of interest. The sequence of the gene
can be found through the accession number link on the product page.
1 tttgaaagaccccacccgtaggtggcaagctagcttaagtaacgccactttgcaaggcat 60 AAACTTTCTGGGGTGGGCATCCACCGTTCGATCGAATTCATTGCGGTGAAACGTTCCGTA 61 ggaaaaatacataactgagaatagaaaagttcagatcaaggtcaggaacaaagaaacagc 120 CCTTTTTATGTATTGACTCTTATCTTTTCAAGTCTAGTTCCAGTCCTTGTTTCTTTGTCG 121 tgaataccaaacaggatatctgtggtaagcggttcctgccccggctcagggccaagaaca 180 ACTTATGGTTTGTCCTATAGACACCATTCGCCAAGGACGGGGCCGAGTCCCGGTTCTTGT 181 gatgagacagctgagtgatgggccaaacaggatatctgtggtaagcagttcctgccccgg 240 CTACTCTGTCGACTCACTACCCGGTTTGTCCTATAGACACCATTCGTCAAGGACGGGGCC 241 ctcggggccaagaacagatggtccccagatgcggtccagccctcagcagtttctagtgaa 300 GAGCCCCGGTTCTTGTCTACCAGGGGTCTACGCCAGGTCGGGAGTCGTCAAAGATCACTT 301 tcatcagatgtttccagggtgccccaaggacctgaaaatgaccctgtaccttatttgaac 360 AGTAGTCTACAAAGGTCCCACGGGGTTCCTGGACTTTTACTGGGACATGGAATAAACTTG 361 taaccaatcagttcgcttctcgcttctgttcgcgcgcttccgctctccgagctcaataaa 420 ATTGGTTAGTCAAGCGAAGAGCGAAGACAAGCGCGCGAAGGCGAGAGGCTCGAGTTATTT 421 agagcccacaacccctcactcggcgcgccagtcttccgatagactgcgtcgcccgggtac 480 TCTCGGGTGTTGGGGAGTGAGCCGCGCGGTCAGAAGGCTATCTGACGCAGCGGGCCCATG 481 ccgtattcccaataaagcctcttgctgtttgcatccgaatcgtggtctcgctgttccttg 540 GGCATAAGGGTTATTTCGGAGAACGACAAACGTAGGCTTAGCACCAGAGCGACAAGGAAC 541 ggagggtctcctctgagtgattgactacccacgacgggggtctttcatttgggggctcgt 600 CCTCCCAGAGGAGACTCACTAACTGATGGGTGCTGCCCCCAGAAAGTAAACCCCCGAGCA psi_plus_pack other(659,1468)>>> | 601 ccgggatttggagacccctgcccagggaccaccgacccaccaccgggaggtaagctggcc 660 GGCCCTAAACCTCTGGGGACGGGTCCCTGGTGGCTGGGTGGTGGCCCTCCATTCGACCGG 661 agcaacttatctgtgtctgtccgattgtctagtgtctatgtttgatgttatgcgcctgcg 720 TCGTTGAATAGACACAGACAGGCTAACAGATCACAGATACAAACTACAATACGCGGACGC 721 tctgtactagttagctaactagctctgtatctggcggacccgtggtggaactgacgagtt 780 AGACATGATCAATCGATTGATCGAGACATAGACCGCCTGGGCACCACCTTGACTGCTCAA 781 ctgaacacccggccgcaaccctgggagacgtcccagggactttgggggccgtttttgtgg 840 GACTTGTGGGCCGGCGTTGGGACCCTCTGCAGGGTCCCTGAAACCCCCGGCAAAAACACC 841 cccgacctgaggaagggagtcgatgtggaatccgaccccgtcaggatatgtggttctggt 900 GGGCTGGACTCCTTCCCTCAGCTACACCTTAGGCTGGGGCAGTCCTATACACCAAGACCA 901 aggagacgagaacctaaaacagttcccgcctccgtctgaatttttgctttcggtttggaa 960 TCCTCTGCTCTTGGATTTTGTCAAGGGCGGAGGCAGACTTAAAAACGAAAGCCAAACCTT 961 ccgaagccgcgcgtcttgtctgctgcagcgctgcagcatcgttctgtgttgtctctgtct 1020 GGCTTCGGCGCGCAGAACAGACGACGTCGCGACGTCGTAGCAAGACACAACAGAGACAGA 1021 gactgtgtttctgtatttgtctgaaaattagggccagactgttaccactcccttaagttt 1080 CTGACACAAAGACATAAACAGACTTTTAATCCCGGTCTGACAATGGTGAGGGAATTCAAA 1081 gaccttaggtcactggaaagatgtcgagcggatcgctcacaaccagtcggtagatgtcaa 1140 CTGGAATCCAGTGACCTTTCTACAGCTCGCCTAGCGAGTGTTGGTCAGCCATCTACAGTT 1141 gaagagacgttgggttaccttctgctctgcagaatggccaacctttaacgtcggatggcc 1200 CTTCTCTGCAACCCAATGGAAGACGAGACGTCTTACCGGTTGGAAATTGCAGCCTACCGG 1201 gcgagacggcacctttaaccgagacctcatcacccaggttaagatcaaggtcttttcacc 1260 CGCTCTGCCGTGGAAATTGGCTCTGGAGTAGTGGGTCCAATTCTAGTTCCAGAAAAGTGG 1261 tggcccgcatggacacccagaccaggtcccctacatcgtgacctgggaagccttggcttt 1320 ACCGGGCGTACCTGTGGGTCTGGTCCAGGGGATGTAGCACTGGACCCTTCGGAACCGAAA 1321 tgacccccctccctgggtcaagccctttgtacaccctaagcctccgcctcctcttcctcc 1380 ACTGGGGGGAGGGACCCAGTTCGGGAAACATGTGGGATTCGGAGGCGGAGGAGAAGGAGG 1381 atccgccccgtctctcccccttgaacctcctcgttcgaccccgcctcgatcctcccttta 1440 TAGGCGGGGCAGAGAGGGGGAACTTGGAGGAGCAAGCTGGGGCGGAGCTAGGAGGGAAAT ORF_4 rf(5)(1449,2216)<<< | 1441 tccagccctcactccttctctaggcgccggaattgatcccccgggctgcaggtcggccgc 1500 AGGTCGGGAGTGAGGAAGAGATCCGCGGCCTTAACTAGGGGGCCCGACGTCCAGCCGGCG 1501 cacgaccggtgccgccaccatcccctgacccacgcccctgacccctcacaaggagacgac 1560 GTGCTGGCCACGGCGGTGGTAGGGGACTGGGTGCGGGGACTGGGGAGTGTTCCTCTGCTG puro marker(1566,2165)>>> | 1561 cttccatgaccgagtacaagcccacggtgcgcctcgccacccgcgacgacgtcccccggg 1620 GAAGGTACTGGCTCATGTTCGGGTGCCACGCGGAGCGGTGGGCGCTGCTGCAGGGGGCCC 1621 ccgtacgcaccctcgccgccgcgttcgccgactaccccgccacgcgccacaccgtcgacc 1680 GGCATGCGTGGGAGCGGCGGCGCAAGCGGCTGATGGGGCGGTGCGCGGTGTGGCAGCTGG 1681 cggaccgccacatcgagcgggtcaccgagctgcaagaactcttcctcacgcgcgtcgggc 1740 GCCTGGCGGTGTAGCTCGCCCAGTGGCTCGACGTTCTTGAGAAGGAGTGCGCGCAGCCCG 1741 tcgacatcggcaaggtgtgggtcgcggacgacggcgccgcggtggcggtctggaccacgc 1800 AGCTGTAGCCGTTCCACACCCAGCGCCTGCTGCCGCGGCGCCACCGCCAGACCTGGTGCG 1801 cggagagcgtcgaagcgggggcggtgttcgccgagatcggcccgcgcatggccgagttga 1860 GCCTCTCGCAGCTTCGCCCCCGCCACAAGCGGCTCTAGCCGGGCGCGTACCGGCTCAACT 1861 gcggttcccggctggccgcgcagcaacagatggaaggcctcctggcgccgcaccggccca 1920 CGCCAAGGGCCGACCGGCGCGTCGTTGTCTACCTTCCGGAGGACCGCGGCGTGGCCGGGT 1921 aggagcccgcgtggttcctggccaccgtcggcgtctcgcccgaccaccagggcaagggtc 1980 TCCTCGGGCGCACCAAGGACCGGTGGCAGCCGCAGAGCGGGCTGGTGGTCCCGTTCCCAG 1981 tgggcagcgccgtcgtgctccccggagtggaggcggccgagcgcgccggggtgcccgcct 2040 ACCCGTCGCGGCAGCACGAGGGGCCTCACCTCCGCCGGCTCGCGCGGCCCCACGGGCGGA 2041 tcctggagacctccgcgccccgcaacctccccttctacgagcggctcggcttcaccgtca 2100 AGGACCTCTGGAGGCGCGGGGCGTTGGAGGGGAAGATGCTCGCCGAGCCGAAGTGGCAGT 2101 ccgccgacgtcgagtgcccgaaggaccgcgcgacctggtgcatgacccgcaagcccggtg 2160 GGCGGCTGCAGCTCACGGGCTTCCTGGCGCGCTGGACCACGTACTGGGCGTTCGGGCCAC 2161 cctgacgcccgccccacgacccgcagcgcccgaccgaaaggagcgcacgaccccatggct 2220 GGACTGCGGGCGGGGTGCTGGGCGTCGCGGGCTGGCTTTCCTCGCGTGCTGGGGTACCGA 2221 ccgaccgaagccgacccgggcggccccgccgaccccgcacccgcccccgaggcccaccga 2280 GGCTGGCTTCGGCTGGGCCCGCCGGGGCGGCTGGGGCGTGGGCGGGGGCTCCGGGTGGCT 2281 ctctagtaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgc 2340 GAGATCATTATCATTAGTTAATGCCCCAGTAATCAAGTATCGGGTATATACCTCAAGGCG 2341 gttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattg 2400 CAATGTATTGAATGCCATTTACCGGGCGGACCGACTGGCGGGTTGCTGGGGGCGGGTAAC 2401 acgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaa 2460 TGCAGTTATTACTGCATACAAGGGTATCATTGCGGTTATCCCTGAAAGGTAACTGCAGTT 2461 tgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgcca 2520 ACCCACCTCATAAATGCCATTTGACGGGTGAACCGTCATGTAGTTCACATAGTATACGGT 2521 agtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtac 2580 TCATGCGGGGGATAACTGCAGTTACTGCCATTTACCGGGCGGACCGTAATACGGGTCATG 2581 atgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattacc 2640 TACTGGAATACCCTGAAAGGATGAACCGTCATGTAGATGCATAATCAGTAGCGATAATGG 2641 atggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacgggga 2700 TACCACTACGCCAAAACCGTCATGTAGTTACCCGCACCTATCGCCAAACTGAGTGCCCCT 2701 tttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgg 2760 AAAGGTTCAGAGGTGGGGTAACTGCAGTTACCCTCAAACAAAACCGTGGTTTTAGTTGCC CMV prom(2787,2868)>>> | 2761 gactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgta 2820 CTGAAAGGTTTTACAGCATTGTTGAGGCGGGGTAACTGCGTTTACCCGCCATCCGCACAT 2821 cggtgggaggtctatataagcagagctggtttagtgaaccgtcagatccgctagcgctac 2880 GCCACCCTCCAGATATATTCGTCTCGACCAAATCACTTGGCAGTCTAGGCGATCGCGATG attR2 other(2937,3037)<<< | attR1 other(2913,3037)>>> | | 2881 cggactcaGATCTAAGCTTCctagcgatatcaacaagtttgtacaaaaaagctgaacgag 2940 GCCTGAGTCTAGATTCGAAGGATCGCTATAGTTGTTCAAACATGTTTTTTCGACTTGCTC 2941 aaacgtaaaatgatataaatatcaatatattaaattagattttgcataaaaaacagacta 3000 TTTGCATTTTACTATATTTATAGTTATATAATTTAATCTAAAACGTATTTTTTGTCTGAT 3001 cataatactgtaaaacacaacatatccagtcactatggcggccgcattaggcaccccagg 3060 GTATTATGACATTTTGTGTTGTATAGGTCAGTGATACCGCCGGCGTAATCCGTGGGGTCC 3061 ctttacactttatgcttccggctcgtataatgtgtggattttgagttaggatccgtcgag 3120 GAAATGTGAAATACGAAGGCCGAGCATATTACACACCTAAAACTCAATCCTAGGCAGCTC CAT marker(3146,3805)>>> | ORF_1 rf(2)(3146,3805)>>> | 3121 attttcaggagctaaggaagctaaaatggagaaaaaaatcactggatataccaccgttga 3180 TAAAAGTCCTCGATTCCTTCGATTTTACCTCTTTTTTTAGTGACCTATATGGTGGCAACT 3181 tatatcccaatggcatcgtaaagaacattttgaggcatttcagtcagttgctcaatgtac 3240 ATATAGGGTTACCGTAGCATTTCTTGTAAAACTCCGTAAAGTCAGTCAACGAGTTACATG 3241 ctataaccagaccgttcagctggatattacggcctttttaaagaccgtaaagaaaaataa 3300 GATATTGGTCTGGCAAGTCGACCTATAATGCCGGAAAAATTTCTGGCATTTCTTTTTATT 3301 gcacaagttttatccggcctttattcacattcttgcccgcctgatgaatgctcatccgga 3360 CGTGTTCAAAATAGGCCGGAAATAAGTGTAAGAACGGGCGGACTACTTACGAGTAGGCCT 3361 attccgtatggcaatgaaagacggtgagctggtgatatgggatagtgttcacccttgtta 3420 TAAGGCATACCGTTACTTTCTGCCACTCGACCACTATACCCTATCACAAGTGGGAACAAT 3421 caccgttttccatgagcaaactgaaacgttttcatcgctctggagtgaataccacgacga 3480 GTGGCAAAAGGTACTCGTTTGACTTTGCAAAAGTAGCGAGACCTCACTTATGGTGCTGCT 3481 tttccggcagtttctacacatatattcgcaagatgtggcgtgttacggtgaaaacctggc 3540 AAAGGCCGTCAAAGATGTGTATATAAGCGTTCTACACCGCACAATGCCACTTTTGGACCG 3541 ctatttccctaaagggtttattgagaatatgtttttcgtctcagccaatccctgggtgag 3600 GATAAAGGGATTTCCCAAATAACTCTTATACAAAAAGCAGAGTCGGTTAGGGACCCACTC 3601 tttcaccagttttgatttaaacgtggccaatatggacaacttcttcgcccccgttttcac 3660 AAAGTGGTCAAAACTAAATTTGCACCGGTTATACCTGTTGAAGAAGCGGGGGCAAAAGTG 3661 catgggcaaatattatacgcaaggcgacaaggtgctgatgccgctggcgattcaggttca 3720 GTACCCGTTTATAATATGCGTTCCGCTGTTCCACGACTACGGCGACCGCTAAGTCCAAGT 3721 tcatgccgtttgtgatggcttccatgtcggcagaatgcttaatgaattacaacagtactg 3780 AGTACGGCAAACACTACCGAAGGTACAGCCGTCTTACGAATTACTTAATGTTGTCATGAC 3781 cgatgagtggcagggcggggcgtaaagatctggatccggcttactaaaagccagataaca 3840 GCTACTCACCGTCCCGCCCCGCATTTCTAGACCTAGGCCGAATGATTTTCGGTCTATTGT 3841 gtatgcgtatttgcgcgctgatttttgcggtataagaatatatactgatatgtatacccg 3900 CATACGCATAAACGCGCGACTAAAAACGCCATATTCTTATATATGACTATACATATGGGC 3901 aagtatgtcaaaaagaggtatgctatgaagcagcgtattacagtgacagttgacagcgac 3960 TTCATACAGTTTTTCTCCATACGATACTTCGTCGCATAATGTCACTGTCAACTGTCGCTG 3961 agctatcagttgctcaaggcatatatgatgtcaatatctccggtctggtaagcacaacca 4020 TCGATAGTCAACGAGTTCCGTATATACTACAGTTATAGAGGCCAGACCATTCGTGTTGGT 4021 tgcagaatgaagcccgtcgtctgcgtgccgaacgctggaaagcggaaaatcaggaaggga 4080 ACGTCTTACTTCGGGCAGCAGACGCACGGCTTGCGACCTTTCGCCTTTTAGTCCTTCCCT 4081 tggctgaggtcgcccggtttattgaaatgaacggctcttttgctgacgagaacaggggct 4140 ACCGACTCCAGCGGGCCAAATAACTTTACTTGCCGAGAAAACGACTGCTCTTGTCCCCGA ccdB other(4147,4452)>>> | 4141 ggtgaaatgcagtttaaggtttacacctataaaagagagagccgttatcgtctgtttgtg 4200 CCACTTTACGTCAAATTCCAAATGTGGATATTTTCTCTCTCGGCAATAGCAGACAAACAC 4201 gatgtacagagtgatattattgacacgcccgggcgacggatggtgatccccctggccagt 4260 CTACATGTCTCACTATAATAACTGTGCGGGCCCGCTGCCTACCACTAGGGGGACCGGTCA 4261 gcacgtctgctgtcagataaagtctcccgtgaactttacccggtggtgcatatcggggat 4320 CGTGCAGACGACAGTCTATTTCAGAGGGCACTTGAAATGGGCCACCACGTATAGCCCCTA 4321 gaaagctggcgcatgatgaccaccgatatggccagtgtgccggtctccgttatcggggaa 4380 CTTTCGACCGCGTACTACTGGTGGCTATACCGGTCACACGGCCAGAGGCAATAGCCCCTT 4381 gaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaacctgatgttc 4440 CTTCACCGACTAGAGTCGGTGGCGCTTTTACTGTAGTTTTTGCGGTAATTGGACTACAAG attR2 other(4493,4593)>>> | attR1 other(4493,4612)<<< | 4441 tggggaatataaatgtcaggctcccttatacacagccagtctgcaggtcgaccatagtga 4500 ACCCCTTATATTTACAGTCCGAGGGAATATGTGTCGGTCAGACGTCCAGCTGGTATCACT 4501 ctggatatgttgtgttttacagtattatgtagtctgttttttatgcaaaatctaatttaa 4560 GACCTATACAACACAAAATGTCATAATACATCAGACAAAAAATACGTTTTAGATTAAATT 4561 tatattgatatttatatcattttacgtttctcgttcagctttcttgtacaaagtggttga 4620 ATATAACTATAAATATAGTAAAATGCAAAGAGCAAGTCGAAAGAACATGTTTCACCAACT XhoI HpaI IRES reg(4645,5227)>>> | | | 4621 tatctgaCTCGAGgttaacgaattccctctccctcccccccccctaacgttactggccga 4680 ATAGACTGAGCTCCAATTGCTTAAGGGAGAGGGAGGGGGGGGGGATTGCAATGACCGGCT ires_emcv reg(4719,5219)>>> | 4681 agccgcttggaataaggccggtgtgcgtttgtctatatgttattttccaccatattgccg 4740 TCGGCGAACCTTATTCCGGCCACACGCAAACAGATATACAATAAAAGGTGGTATAACGGC 4741 tcttttggcaatgtgagggcccggaaacctggccctgtcttcttgacgagcattcctagg 4800 AGAAAACCGTTACACTCCCGGGCCTTTGGACCGGGACAGAAGAACTGCTCGTAAGGATCC 4801 ggtctttcccctctcgccaaaggaatgcaaggtctgttgaatgtcgtgaaggaagcagtt 4860 CCAGAAAGGGGAGAGCGGTTTCCTTACGTTCCAGACAACTTACAGCACTTCCTTCGTCAA 4861 cctctggaagcttcttgaagacaaacaacgtctgtagcgaccctttgcaggcagcggaac 4920 GGAGACCTTCGAAGAACTTCTGTTTGTTGCAGACATCGCTGGGAAACGTCCGTCGCCTTG 4921 cccccacctggcgacaggtgcctctgcggccaaaagccacgtgtataagatacacctgca 4980 GGGGGTGGACCGCTGTCCACGGAGACGCCGGTTTTCGGTGCACATATTCTATGTGGACGT 4981 aaggcggcacaaccccagtgccacgttgtgagttggatagttgtggaaagagtcaaatgg 5040 TTCCGCCGTGTTGGGGTCACGGTGCAACACTCAACCTATCAACACCTTTCTCAGTTTACC 5041 ctctcctcaagcgtattcaacaaggggctgaaggatgcccagaaggtaccccattgtatg 5100 GAGAGGAGTTCGCATAAGTTGTTCCCCGACTTCCTACGGGTCTTCCATGGGGTAACATAC 5101 ggatctgatctggggcctcggtgcacatgctttacatgtgtttagtcgaggttaaaaaaa 5160 CCTAGACTAGACCCCGGAGCCACGTGTACGAAATGTACACAAATCAGCTCCAATTTTTTT ORF_2 rf(2)(5219,5950)>>> | 5161 cgtctaggccccccgaaccacggggacgtggttttcctttgaaaaacacgatgataatat 5220 GCAGATCCGGGGGGCTTGGTGCCCCTGCACCAAAAGGAAACTTTTTGTGCTACTATTATA EGFP reporter(5231,5947)>>> | 5221 ggccacaaccatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggt 5280 CCGGTGTTGGTACCACTCGTTCCCGCTCCTCGACAAGTGGCCCCACCACGGGTAGGACCA 5281 cgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcga 5340 GCTCGACCTGCCGCTGCATTTGCCGGTGTTCAAGTCGCACAGGCCGCTCCCGCTCCCGCT 5341 tgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgcc 5400 ACGGTGGATGCCGTTCGACTGGGACTTCAAGTAGACGTGGTGGCCGTTCGACGGGCACGG 5401 ctggcccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgctaccccga 5460 GACCGGGTGGGAGCACTGGTGGGACTGGATGCCGCACGTCACGAAGTCGGCGATGGGGCT 5461 ccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcg 5520 GGTGTACTTCGTCGTGCTGAAGAAGTTCAGGCGGTACGGGCTTCCGATGCAGGTCCTCGC 5521 caccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgaggg 5580 GTGGTAGAAGAAGTTCCTGCTGCCGTTGATGTTCTGGGCGCGGCTCCACTTCAAGCTCCC 5581 cgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacat 5640 GCTGTGGGACCACTTGGCGTAGCTCGACTTCCCGTAGCTGAAGTTCCTCCTGCCGTTGTA 5641 cctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaa 5700 GGACCCCGTGTTCGACCTCATGTTGATGTTGTCGGTGTTGCAGATATAGTACCGGCTGTT 5701 gcagaagaacggcatcaaggcgaacttcaagatccgccacaacatcgaggacggcagcgt 5760 CGTCTTCTTGCCGTAGTTCCGCTTGAAGTTCTAGGCGGTGTTGTAGCTCCTGCCGTCGCA 5761 gcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcc 5820 CGTCGAGCGGCTGGTGATGGTCGTCTTGTGGGGGTAGCCGCTGCCGGGGCACGACGACGG 5821 cgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcga 5880 GCTGTTGGTGATGGACTCGTGGGTCAGGCGGGACTCGTTTCTGGGGTTGCTCTTCGCGCT 5881 tcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagct 5940 AGTGTACCAGGACGACCTCAAGCACTGGCGGCGGCCCTAGTGAGAGCCGTACCTGCTCGA 5941 gtacaagtaatgaattaattaagaattatcaccgcttctattcagccagtaaggcctgtc 6000 CATGTTCATTACTTAATTAATTCTTAATAGTGGCGAAGATAAGTCGGTCATTCCGGACAG 6001 tttaatggcctccggcatgagacactttctagagggcccGTTTAAACGaattcgttaggc 6060 AAATTACCGGAGGCCGTACTCTGTGAAAGATCTCCCGGGCAAATTTGCTTAAGCAATCCG ClaI | 6061 cattaaggcctgtcgacaagcggccgcctcggccaaacatcgataaaataaaagatttta 6120 GTAATTCCGGACAGCTGTTCGCCGGCGGAGCCGGTTTGTAGCTATTTTATTTTCTAAAAT 6121 tttagtctccagaaaaaggggggaatgaaagaccccacctgtaggtttggcaagctagct 6180 AAATCAGAGGTCTTTTTCCCCCCTTACTTTCTGGGGTGGACATCCAAACCGTTCGATCGA 5_LTR2 other(6186,6737)>>> | 6181 taagtaacgccattttgcaaggcatggaaaaatacataactgagaatagagaagttcaga 6240 ATTCATTGCGGTAAAACGTTCCGTACCTTTTTATGTATTGACTCTTATCTCTTCAAGTCT 6241 tcaaggtcaggaacagatggaacagctgaatatgggccaaacaggatatctgtggtaagc 6300 AGTTCCAGTCCTTGTCTACCTTGTCGACTTATACCCGGTTTGTCCTATAGACACCATTCG 6301 agttcctgccccggctcagggccaagaacagatggaacagctgaatatgggccaaacagg 6360 TCAAGGACGGGGCCGAGTCCCGGTTCTTGTCTACCTTGTCGACTTATACCCGGTTTGTCC 6361 atatctgtggtaagcagttcctgccccggctcagggccaagaacagatggtccccagatg 6420 TATAGACACCATTCGTCAAGGACGGGGCCGAGTCCCGGTTCTTGTCTACCAGGGGTCTAC 6421 cggtccagccctcagcagtttctagagaaccatcagatgtttccagggtgccccaaggac 6480 GCCAGGTCGGGAGTCGTCAAAGATCTCTTGGTAGTCTACAAAGGTCCCACGGGGTTCCTG 6481 ctgaaatgaccctgtgccttatttgaactaaccaatcagttcgcttctcgcttctgttcg 6540 GACTTTACTGGGACACGGAATAAACTTGATTGGTTAGTCAAGCGAAGAGCGAAGACAAGC 6541 cgcgcttctgctccccgagctcaataaaagagcccacaacccctcactcggggcgccagt 6600 GCGCGAAGACGAGGGGCTCGAGTTATTTTCTCGGGTGTTGGGGAGTGAGCCCCGCGGTCA 6601 cctccgattgactgagtcgcccgggtacccgtgtatccaataaaccctcttgcagttgca 6660 GGAGGCTAACTGACTCAGCGGGCCCATGGGCACATAGGTTATTTGGGAGAACGTCAACGT 6661 tccgacttgtggtctcgctgttccttgggagggtctcctctgagtgattgactacccgtc 6720 AGGCTGAACACCAGAGCGACAAGGAACCCTCCCAGAGGAGACTCACTAACTGATGGGCAG 6721 agcgggggtctttcatttgggggctcgtccgggatcgggagacccctgcccagggaccac 6780 TCGCCCCCAGAAAGTAAACCCCCGAGCAGGCCCTAGCCCTCTGGGGACGGGTCCCTGGTG 6781 cgacccaccaccgggaggtaagctggctgcctcgcgcgtttcggtgatgacggtgaaaac 6840 GCTGGGTGGTGGCCCTCCATTCGACCGACGGAGCGCGCAAAGCCACTACTGCCACTTTTG 6841 ctctgacacatgcagctcccggagacggtcacagcttgtctgtaagcggatgccgggagc 6900 GAGACTGTGTACGTCGAGGGCCTCTGCCAGTGTCGAACAGACATTCGCCTACGGCCCTCG 6901 agacaagcccgtcagggcgcgtcagcgggtgttggcgggtgtcggggcgcagccatgacc 6960 TCTGTTCGGGCAGTCCCGCGCAGTCGCCCACAACCGCCCACAGCCCCGCGTCGGTACTGG loxP other(6990,7023)<<< | 6961 cagtcacgtagcgatagcggagtgtactgataacttcgtataatgtatgctatacgaagt 7020 GTCAGTGCATCGCTATCGCCTCACATGACTATTGAAGCATATTACATACGATATGCTTCA 7021 tattaggtctgaagaggagtttacgtccagccaagctagggccgcgatccggaaccctta 7080 ATAATCCAGACTTCTCCTCAAATGCAGGTCGGTTCGATCCCGGCGCTAGGCCTTGGGAAT loxP other(7083,7116)<<< | 7081 atataacttcgtataatgtatgctatacgaagttatcagtactggcttaactatgcggca 7140 TATATTGAAGCATATTACATACGATATGCTTCAATAGTCATGACCGAATTGATACGCCGT 7141 tcagagcagattgtactgagagtgcaccatatgcggtgtgaaataccgcacagatgcgta 7200 AGTCTCGTCTAACATGACTCTCACGTGGTATACGCCACACTTTATGGCGTGTCTACGCAT 7201 aggagaaaataccgcatcaggcgctcttccgcttcctcgctcactgactcgctgcgctcg 7260 TCCTCTTTTATGGCGTAGTCCGCGAGAAGGCGAAGGAGCGAGTGACTGAGCGACGCGAGC 7261 gtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccaca 7320 CAGCAAGCCGACGCCGCTCGCCATAGTCGAGTGAGTTTCCGCCATTATGCCAATAGGTGT 7321 gaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaac 7380 CTTAGTCCCCTATTGCGTCCTTTCTTGTACACTCGTTTTCCGGTCGTTTTCCGGTCCTTG pBR322 origin(7392,8011)<<< | 7381 cgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcac 7440 GCATTTTTCCGGCGCAACGACCGCAAAAAGGTATCCGAGGCGGGGGGACTGCTCGTAGTG 7441 aaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcg 7500 TTTTTAGCTGCGAGTTCAGTCTCCACCGCTTTGGGCTGTCCTGATATTTCTATGGTCCGC 7501 tttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatac 7560 AAAGGGGGACCTTCGAGGGAGCACGCGAGAGGACAAGGCTGGGACGGCGAATGGCCTATG 7561 ctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtat 7620 GACAGGCGGAAAGAGGGAAGCCCTTCGCACCGCGAAAGAGTATCGAGTGCGACATCCATA 7621 ctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcag 7680 GAGTCAAGCCACATCCAGCAAGCGAGGTTCGACCCGACACACGTGCTTGGGGGGCAAGTC 7681 cccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgac 7740 GGGCTGGCGACGCGGAATAGGCCATTGATAGCAGAACTCAGGTTGGGCCATTCTGTGCTG 7741 ttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggt 7800 AATAGCGGTGACCGTCGTCGGTGACCATTGTCCTAATCGTCTCGCTCCATACATCCGCCA 7801 gctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggt 7860 CGATGTCTCAAGAACTTCACCACCGGATTGATGCCGATGTGATCTTCCTGTCATAAACCA 7861 atctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggc 7920 TAGACGCGAGACGACTTCGGTCAATGGAAGCCTTTTTCTCAACCATCGAGAACTAGGCCG 7921 aaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcaga 7980 TTTGTTTGGTGGCGACCATCGCCACCAAAAAAACAAACGTTCGTCGTCTAATGCGCGTCT 7981 aaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaac 8040 TTTTTTCCTAGAGTTCTTCTAGGAAACTAGAAAAGATGCCCCAGACTGCGAGTCACCTTG 8041 gaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatc 8100 CTTTTGAGTGCAATTCCCTAAAACCAGTACTCTAATAGTTTTTCCTAGAAGTGGATCTAG 8101 cttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtct 8160 GAAAATTTAATTTTTACTTCAAAATTTAGTTAGATTTCATATATACTCATTTGAACCAGA amp marker(8166,9026)<<< | ORF_3 rf(5)(8166,9026)<<< | 8161 gacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttca 8220 CTGTCAATGGTTACGAATTAGTCACTCCGTGGATAGAGTCGCTAGACAGATAAAGCAAGT 8221 tccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatct 8280 AGGTATCAACGGACTGAGGGGCAGCACATCTATTGATGCTATGCCCTCCCGAATGGTAGA 8281 ggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagca 8340 CCGGGGTCACGACGTTACTATGGCGCTCTGGGTGCGAGTGGCCGAGGTCTAAATAGTCGT 8341 ataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctcc 8400 TATTTGGTCGGTCGGCCTTCCCGGCTCGCGTCTTCACCAGGACGTTGAAATAGGCGGAGG 8401 atccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttg 8460 TAGGTCAGATAATTAACAACGGCCCTTCGATCTCATTCATCAAGCGGTCAATTATCAAAC 8461 cgcaacgttgttgccattgctgcaggcatcgtggtgtcacgctcgtcgtttggtatggct 8520 GCGTTGCAACAACGGTAACGACGTCCGTAGCACCACAGTGCGAGCAGCAAACCATACCGA 8521 tcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaa 8580 AGTAAGTCGAGGCCAAGGGTTGCTAGTTCCGCTCAATGTACTAGGGGGTACAACACGTTT 8581 aaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgtta 8640 TTTCGCCAATCGAGGAAGCCAGGAGGCTAGCAACAGTCTTCATTCAACCGGCGTCACAAT 8641 tcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgc 8700 AGTGAGTACCAATACCGTCGTGACGTATTAAGAGAATGACAGTACGGTAGGCATTCTACG 8701 ttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccg 8760 AAAAGACACTGACCACTCATGAGTTGGTTCAGTAAGACTCTTATCACATACGCCGCTGGC 8761 agttgctcttgcccggcgtcaacacgggataataccgcgccacatagcagaactttaaaa 8820 TCAACGAGAACGGGCCGCAGTTGTGCCCTATTATGGCGCGGTGTATCGTCTTGAAATTTT 8821 gtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttg 8880 CACGAGTAGTAACCTTTTGCAAGAAGCCCCGCTTTTGAGAGTTCCTAGAATGGCGACAAC 8881 agatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttc 8940 TCTAGGTCAAGCTACATTGGGTGAGCACGTGGGTTGACTAGAAGTCGTAGAAAATGAAAG 8941 accagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagg 9000 TGGTCGCAAAGACCCACTCGTTTTTGTCCTTCCGTTTTACGGCGTTTTTTCCCTTATTCC 9001 gcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttat 9060 CGCTGTGCCTTTACAACTTATGAGTATGAGAAGGAAAAAGTTATAATAACTTCGTAAATA amp prom(9068,9096)<<< | 9061 cagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaata 9120 GTCCCAATAACAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTAT 9121 ggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatc 9180 CCCCAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTGCAGATTCTTTGGTAATAATAG 9181 atgacattaacctataaaaataggcgtatcacgaggccctttcgtcttcaagaattgcta 9240 TACTGTAATTGGATATTTTTATCCGCATAGTGCTCCGGGAAAGCAGAAGTTCTTAACGAT 9241 gcaattgctagcaattgctagcaattcataccagatcaccgaaaactgtcctccaaatgt 9300 CGTTAACGATCGTTAACGATCGTTAAGTATGGTCTAGTGGCTTTTGACAGGAGGTTTACA 9301 gtccccctcacactcccaaattcgcgggcttctgcctcttagaccactctaccctattcc 9360 CAGGGGGAGTGTGAGGGTTTAAGCGCCCGAAGACGGAGAATCTGGTGAGATGGGATAAGG 9361 ccacactcaccggagccaaagccgcggcccttccgtttctttgct 9405 GGTGTGAGTGGCCTCGGTTTCGGCGCCGGGAAGGCAAAGAAACGA
LOCUS dna 9405 bp FEATURES Location/Qualifiers Other Gene 659..1468 /gene="psi_plus_pack other" ORF 1449..2216 /sequence="ORF_4 rf(5)" Marker 1566..2165 /gene="puro marker" Promoter 2787..2868 /gene="CMV prom" Other Gene 2913..3037 /gene="attR1 other" Other Gene 2937..3037 /gene="attR2 other" Marker 3146..3805 /gene="CAT marker" ORF 3146..3805 /sequence="ORF_1 rf(2)" Other Gene 4147..4452 /gene="ccdB other" Other Gene 4493..4612 /gene="attR1 other" Other Gene 4493..4593 /gene="attR2 other" misc_binding 4628..4633 /dbxref="REBASE:XhoI" misc_binding 4634..4639 /dbxref="REBASE:HpaI" Regulatory_Seq 4645..5227 /gene="IRES reg" Regulatory_Seq 4719..5219 /gene="ires_emcv reg" ORF 5219..5950 /sequence="ORF_2 rf(2)" Reporter 5231..5947 /gene="EGFP reporter" misc_binding 6099..6104 /dbxref="REBASE:ClaI" Other Gene 6186..6737 /gene="5_LTR2 other" Other Gene 6990..7023 /gene="loxP other" Other Gene 7083..7116 /gene="loxP other" Rep_Origin 7392..8011 /gene="pBR322 origin" Marker 8166..9026 /gene="amp marker" ORF 8166..9026 /sequence="ORF_3 rf(5)" Promoter 9068..9096 /gene="amp prom" BASE COUNT 2269 a 2580 c 2378 g 2178 t 0 others