CRISPR Custom Targeted Lentiviral sgRNA Library
Studying a large family of genes, or a complex pathway? Now you can knock out, activate, or repress up to 100 target genes of your choice with a single Custom Targeted sgRNA Lentivector/Lentivirus pool. Our targeted sgRNA library provides you with both a 100 μg pool of 300 different sgRNA plasmids (i.e. 3 gRNA for each target gene to ensure effective targeting) and 1 ml of ≥1 x 107 IU/mL pooled lentivirus. To get started on your Custom sgRNA Library, click the Inquire button below.
Applications
Simultaneously target hundreds of genes for:
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Gene Knockout with any Cas9 Nuclease (check out abm’s Cas9 Expressing Stable Cell Lines)
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Gene Activation with dCas9-SAM
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Gene Repression with dCas9-KRAB
Additional Resources:
"Construct works beautifully, definitely will get more if needed."
Emily Chen, UCLA, CRISPR ConstructsService Details
| Service Name | Unit | Cat. No. | Price |
|---|---|---|---|
| CRISPR Custom Targeted Lentiviral sgRNA Library | 1 Library | C209 | $9950.00 |
Additional Info
| Vector | pLenti-U6-sgRNA-PGK-Neo |
| Mammalian Selection | Neomycin |
| Format | Pooled Lentiviral Vector Plasmid DNA and Pooled Lentivirus |
| Appearance | Liquid |
| Storage | -80°C or below |
| Shelf Life | 1 year (when at -80°C or below in a non-frost free freezer) |
| Shipping | Shipped on Dry Ice |
| Quality Control | Restriction Enzyme Digest and batch Sanger Sequencing |
| Notes | This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. |
Related Products
Documents
FAQs
| If I place an order for 100 target genes, what are the deliverables? |
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We will deliver 1 vial of ~100 ug plasmids, which is a pool of 300 sgRNA plasmids targeting 100 genes (i.e. 3 sgRNAs for each target gene), and 1 ml of packaged virus at >1 x 107 IU/ml titre. The deliverables will not be individual tubes of sgRNAs. It will be a single pool of all the sgRNA constructs.
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| What is the lead time? |
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The lead time is around 4-6 weeks.
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| Is Cas9 included in the package? |
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No, this can be ordered separately. We recommend co-transfection with Cas9 Nuclease NLS Protein (Cat. No. K030 or K130) due to the number of sgRNA constructs included in the pool.
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| How many vials of Cas9 Nuclease will be required? |
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The sgRNA plasmids will be supplied as a pool of lentiviral vectors, and a single vial of at least 1 mL of packaged virus will be supplied. Only a single tube of Cas9 Nuclease Lentiviral Vector (Cat. No. K002) is required as it can be amplified by transforming into E. coli and isolating the DNA. If Cas9 Nuclease Lentivirus (Cat. No. K003) is ordered, the amount required will depend on how many different samples the end user is interested in infecting. If you are only working with a few cell ines, then 1 vial may be sufficient to infect the cell to generate stable cell lines expressing Cas9 Nuclease for use with the pool. If you are working with more cell lines, then more than 1 vial may be required.
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| What species is this service valid for? |
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Human, mouse or rat. For other species, please inquire.
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Citations
| 01 | Kang, Y. J. et al. "Regulation of NKT cell-mediated immune responses to tumours and liver inflammation by mitochondrial PGAM5-Drp1 signalling." Nat. Commun. (2015) 6:8371 doi: 10.1038/ncomms9371 |
| 02 | Jiang, G. et al. "Isorhapontigenin (ISO) inhibits invasive bladder cancer (BC) formation in vivo and human BC invasion in vitro by targeting STAT1/FOXO1 Axis." Cancer Prev Res. Published Online First April 14, 2016.doi: 10.1158/1940-6207.CAPR-15-0338 |
| 03 | Okugawa, Y. et al. "Clinical significance of SNORA42 as an oncogene and a prognostic biomarker in colorectal cancer." Gut (2015)doi:10.1136/gutjnl-2015-309359 |