Cas9 Nuclease Lentiviral Vector
Cat. No.
K002
Unit
10 µg
Price
$205.00
Specifications
| SKU | K002 |
| Name | Cas9 Nuclease Lentiviral Vector |
| Unit | 10 µg |
| Caution | This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. |
| Vector | pLenti-EF1a-Cas9-Puro |
| Description |
This Cas9 nuclease can be used with a target-specific sgRNA to bind DNA and create a double-stranded cut. |
| Format | Vector |
| Shipping Conditions |
Shipped at ambient temperature |
| Note |
Cas9 WT includes N-terminal SV40 NLS and C-terminal Nucleoplasmin NLS |
Documents
There are no Documents for this product yet!
FAQs
| How long after transduction can the infection efficiency be observed? | |
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You can observe transduction efficiency from 48 hours up to 5 days after infection.
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| I need to design a QPCR primer to detect the expression level of Cas9 in my target cells, can you please provide the Cas9 sequence info? | |
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We are unable to supply the full sequence due to propriety reasons. However, we can supply a short ~600-800bp region of the Cas9 sequence for you to design the qPCR primer from. We will be happy to provide this information once an order has been processed.
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| What are the primers to use for SV40T and SV40T tsA58 detection? | |
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PCR primers:
SV40T Forward Primer Sequence
5’ AGCCTGTAGAACCAAACATT 3'
SV40T Reverse Primer Sequence
5’ CTGCTGACTCTCAACATTCT 3'
The two primers should amplify the region between 3677-4468bp, giving a 792bp fragment.
|
| What is the sequence of the SV40 large T antigen? | |
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This information can be accessed on this page by clicking on "pLenti-SV40-T" under vector map. The Large T antigen is at position 5079-5927.
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| Can you provide the sequence for this plasmid? | |
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We are unable to release the sequence of our Cas9 Nickase / Nuclease as it has been codon optimized for expression and this is proprietary information. A vector map can be located however by clicking on the vector name.
|
| For G221 and LV620, what does the 'V12' in RasV12 mean? | |
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The V12 means that amino acid # 12 is mutated from a Valine to a Glycine. Other than that, the sequence matches the coding region of HRAS perfectly (NM_005343).
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| Where is the SV40T tsA58 gene sequence? | |
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The SV40T tsA58 gene is located between 3138-5264bp, with the Alanine-to-Valine mutation at amino acid 438.
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References
- The Board of Regents of the University of Texas System., . "GENERATION OF GENETICALLY ENGINEERED ANIMALS BY CRISPR/CAS9 GENOME EDITING IN SPERMATOGONIAL STEM CELLS" United States Patent Application 20190134227. Retrieved from : (2019).
- Mohler, . "U" S. Patent Application No. 0201442 A1 : (2019).
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