pLenti-EF1a-Cas9-Puro
| Vector Name | pLenti-EF1a-Cas9-Puro |
| VectorType | Lentiviral Vector |
| Antibiotic Information | Ampicillin |
| Additional Information | No Additional Information |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacggg tctctctggt
2461 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
2521 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
2581 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
2641 cagggacctg aaagcgaaag ggaaaccaga gctctctcga cgcaggactc ggcttgctga
2701 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
2761 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
2821 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
2881 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
2941 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
3001 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
3061 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
3121 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
3181 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
3241 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
3301 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcctcaatg acgctgacgg
3361 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
3421 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
3481 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
3541 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
3601 tggaacagat tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
3661 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
3721 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
3781 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
3841 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
3901 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga
3961 gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc ggttaacttt
4021 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
4081 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttccgat
4141 cacgagacta gcctcgagag ctagcggctc cggtgcccgt cagtgggcag agcgcacatc
4201 gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg cctagagaag
4261 gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg
4321 tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt
4381 tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc tctttacggg
4441 ttatggccct tgcgtgcctt gaattacttc cactggctgc agtacgtgat tcttgatccc
4501 gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt gcgcttaagg agccccttcg
4561 cctcgtgctt gagttgaggc ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg
4621 caccttcgcg cctgtctcgc tgctttcgat aagtctctag ccatttaaaa tttttgatga
4681 cctgctgcga cgcttttttt ctggcaagat agtcttgtaa atgcgggcca agatctgcac
4741 actggtattt cggtttttgg ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca
4801 tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa
4861 gctggccggc ctgctctggt gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg
4921 gcaaggctgg cccggtcggc accagttgcg tgagcggaaa gatggccgct tcccggccct
4981 gctgcaggga gctcaaaatg gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc
5041 acacaaagga aaagggcctt tccgtcctca gccgtcgctt catgtgactc cacggagtac
5101 cgggcgccgt ccaggcacct cgattagttc tcgagctttt ggagtacgtc gtctttaggt
5161 tggggggagg ggttttatgc gatggagttt ccccacactg agtgggtgga gactgaagtt
5221 aggccagctt ggcacttgat gtaattctcc ttggaatttg ccctttttga gtttggatct
5281 tggttcattc tcaagccttc ttccatttca ggtgtcgtga gcgatcgcca tggctcccaa
5341 gaagaagcga aaggtgggca tccacggcgt gcccgctgcc gacaaaaagt atagtatcgg
5401 actggatatt ggcactaaca gcgtgggatg ggccgtcatc accgacgagt acaaagtgcc
5461 aagcaagaag ttcaaggtcc tgggaaacac cgatagacac agtatcaaga aaaatctgat
5521 tggagccctg ctgttcgact caggggagac agctgaagca actaggctga aaagaacagc
5581 taggagacgg tatactcgcc gaaagaatcg gatctgctac ctccaggaga ttttctccaa
5641 cgaaatggcc aaggtggacg atagtttctt tcatcgcctg gaggaatcat tcctggtcga
5701 ggaagataag aaacacgaga ggcatcccat ctttggcaac attgtggacg aggtcgctta
5761 tcacgaaaag taccctacaa tctatcatct gcggaagaaa ctggtggaca gcactgataa
5821 ggcagacctg cgcctgatct atctggccct ggctcacatg attaagttca gggggcattt
5881 tctgatcgag ggcgatctga acccagacaa ttccgatgtg gacaagctgt tcatccagct
5941 ggtccagaca tacaatcagc tgtttgagga aaaccccatt aatgcatctg gggtggacgc
6001 aaaagccatc ctgagtgcca gactgtctaa gagtaggaga ctggagaacc tgatcgctca
6061 gctgccaggc gaaaagaaaa acggcctgtt tggaaatctg attgcactgt cactgggact
6121 gacccccaac ttcaagagca attttgatct ggccgaggac gctaagctcc agctgagcaa
6181 ggacacctac gacgatgacc tggataacct gctggctcag atcggcgatc agtacgcaga
6241 cctgttcctg gccgctaaga atctgtctga cgccatcctg ctgagtgata ttctgagagt
6301 gaacaccgag attacaaaag cccccctgtc agctagcatg atcaagagat atgacgagca
6361 ccatcaggat ctgaccctgc tgaaggctct ggtgcggcag cagctgcctg agaagtacaa
6421 agaaatcttc tttgatcaga gcaagaatgg gtacgccggc tatattgacg gcggagcttc
6481 ccaggaggag ttctacaagt ttatcaaacc tattctggag aagatggacg gcactgagga
6541 actgctggtg aaactgaatc gggaagacct gctgcggaag cagcgcacct tcgataacgg
6601 cagcatccct caccagattc atctgggaga gctgcacgca atcctgcggc gccaggaaga
6661 cttctaccca tttctgaagg ataaccggga gaagatcgaa aaaattctga ctttccgcat
6721 cccctactat gtggggcctc tggcaagagg caattcccgg tttgcctgga tgacccgcaa
6781 gtctgaggaa acaatcactc cctggaactt cgaggaagtg gtcgataagg gcgcttccgc
6841 acagtctttc attgagagga tgacaaattt tgacaagaac ctgccaaatg aaaaagtgct
6901 gcccaagcac agcctgctgt acgagtattt caccgtctat aacgaactga caaaggtgaa
6961 atacgtcact gagggcatga gaaagcctgc cttcctgtcc ggagaacaga agaaagctat
7021 cgtggacctg ctgtttaaaa ccaatcggaa ggtgacagtc aagcagctga aagaggacta
7081 cttcaagaaa attgaatgtt tcgattctgt ggagatcagt ggggtcgaag acaggtttaa
7141 cgcctctctg ggcacctacc acgatctgct gaagatcatt aaggataaag acttcctgga
7201 caacgaggaa aatgaggaca tcctggagga cattgtgctg accctgacac tgtttgagga
7261 tcgggaaatg atcgaggaac gcctgaagac ctacgcccat ctgttcgatg acaaagtgat
7321 gaaacagctg aagcgaagga gatacactgg gtggggccga ctgagcagga agctgatcaa
7381 tggcattcgc gacaaacaga gtggaaagac aatcctggac tttctgaagt cagatggctt
7441 cgctaacagg aattttatgc agctgattca cgatgactct ctgactttca aagaggacat
7501 ccagaaggca caggtgtccg gacaggggga ctctctgcac gagcatatcg caaacctggc
7561 cgggagccct gccatcaaga aaggcatcct ccagaccgtg aaggtggtgg acgagctggt
7621 gaaagtcatg ggaagacata agccagaaaa catcgtgatt gagatggcca gggagaatca
7681 gaccacacag aaagggcaga agaactctcg ggagcgcatg aaacgcatcg aggaaggaat
7741 taaggaactg gggagtcaga tcctgaaaga gcaccccgtg gaaaacacac agctccagaa
7801 tgagaagctg tatctgtact acctccagaa tggccgcgat atgtacgtgg accaggagct
7861 ggatattaac cgactgtcag attatgacgt ggatcatatc gtcccacagt cattcctgaa
7921 agatgacagc attgacaata aggtgctgac ccgcagcgac aaaaaccgag gaaagagtga
7981 taatgtcccc tcagaggaag tggtcaagaa aatgaagaac tactggaggc agctgctgaa
8041 tgccaaactg atcacccagc gaaagtttga taacctgaca aaagctgaga gggggggcct
8101 gtccgaactg gacaaagcag gcttcatcaa gcgacagctg gtggagacaa ggcagatcac
8161 aaagcacgtc gctcagatcc tggacagcag gatgaacacc aagtacgatg agaatgacaa
8221 actgatccgg gaagtgaagg tcattacact gaagtcaaaa ctggtgagcg actttaggaa
8281 agatttccag ttctacaagg tcagagagat caacaactac caccatgctc atgacgcata
8341 cctgaacgca gtggtcggga ctgccctgat taagaaatac cctaaactgg agtctgagtt
8401 cgtgtacggc gactataagg tgtacgatgt cagaaaaatg atcgccaaga gcgagcagga
8461 aattggcaaa gccaccgcta agtatttctt ttactccaac atcatgaatt tctttaagac
8521 tgagatcacc ctggcaaatg gcgaaatccg aaagaggcca ctgattgaga ctaacggaga
8581 gacaggggaa atcgtgtggg acaaaggaag agattttgct accgtgcgga aggtcctgag
8641 tatgccccaa gtgaatattg tcaagaaaac agaggtgcag actggagggt tcagtaagga
8701 atcaattctg cctaaacgca acagcgataa gctgatcgcc cgaaagaaag actgggaccc
8761 caagaagtat ggcggattcg actccccaac cgtggcttac tctgtcctgg tggtcgcaaa
8821 ggtggagaag ggaaaaagca agaaactgaa atccgtcaag gaactgctgg ggatcacaat
8881 tatggagagg agcagcttcg aaaagaatcc tatcgatttt ctggaggcca aagggtataa
8941 ggaagtgaag aaagacctga tcatcaagct gccaaagtac tctctgtttg agctggaaaa
9001 cggcagaaag cggatgctgg caagtgccgg cgagctgcaa aaaggaaatg aactggccct
9061 gccctcaaag tacgtgaact tcctgtatct ggctagccac tacgagaagc tgaaaggctc
9121 ccctgaggat aacgaacaga aacagctgtt tgtggagcag cacaagcatt atctggacga
9181 gatcattgaa cagattagcg agttctccaa acgcgtgatc ctggctgacg caaatctgga
9241 taaggtcctg tctgcataca acaaacacag ggacaagcca atcagagagc aggccgaaaa
9301 tatcattcat ctgttcactc tgaccaacct gggagccccc gcagccttca agtattttga
9361 cactaccatc gatcgcaaac gatacacaag cactaaggag gtgctggatg ctaccctgat
9421 ccaccagagc attactgggc tgtacgagac aaggatcgac ctgtcccagc tggggggaga
9481 caaacgccca gccgccacca agaaagcagg acaggcaaag aagaagaaga cgcgtcagtg
9541 tactaattat gctctcttga aattggctgg agatgttgag agcaacccag gtcccatgac
9601 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac
9661 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca
9721 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg
9781 caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt
9841 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg
9901 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc
9961 gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc
10021 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac
10081 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt
10141 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgagttta
10201 aacgtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
10261 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
10321 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
10381 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
10441 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
10501 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
10561 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca
10621 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
10681 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
10741 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct
10801 ggaattcgag ctcggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
10861 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
10921 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
10981 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
11041 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
11101 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg
11161 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
11221 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
11281 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct
11341 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
11401 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
11461 aggcctaggc ttttgcgtcg agacgtaccc aattcgccct atagtgagtc gtattacgcg
11521 cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
11581 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
11641 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgcgacgc gccctgtagc
11701 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
11761 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
11821 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
11881 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
11941 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
12001 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
12061 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
12121 aaaatattaa cgtttacaat ttcc