Rabbit Polyclonal Antibody Production

abm offers the most competitive price for quality, custom, polyclonal antibody production. Our services include peptide synthesis, immunization of 2 rabbits, and peptide affinity purification of positive serum. It is all included and there is absolutely no hidden additional cost. Based on our experience, the success rate of generating a quality polyclonal antibody is over 70% with one peptide antigen and over 95% for two peptide antigen.

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"I am a fan of abm: great product quality, great price, great rewards and great experience."

Dr. Vincent Emond, Centre de recherche du CHU de Québec CHUL

Service Details

	Description	Cat. No.	Unit	Price
Standard Service	Customer provides antigens (5mg of >90% purity peptide or 5mg recombinant protein). KLH conjugation and immunization of up to 2 rabbits (most service providers charge an extra $100.00 for KLH conjugation). ELISA assay for titer until it reaches 1:10,000 or better. Peptide affinity purification (2-3ml purified antibody), most service providers charge an extra $500.00 for this step.	C090	1 Service	Inquire
Complete Service	Customer provides gene name or gene/protein sequence. Peptide is designed and synthesized. KLH conjugation and immunization of up to 2 rabbits (most service providers charge an extra $100.00 for KLH conjugation). ELISA assay for titer until it reaches 1:10,000 or better. Peptide affinity purification (2-3ml purified antibody), most service providers charge an extra $500.00 for this step.	C091	1 Service	Inquire
Phospho-Peptide Service	Production of an antibody against a phosphorylated peptide.	C089	1 Service	Inquire

Additional Info

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Custom Antibody Production Services Flyer

FAQs

Can you produce an antibody against a native protein?

Yes, however, in order to guarantee the antibody is effective against the native protein, purified native protein must be used for immunization. Recombinant protein or peptide extracts can also be used to immunize, but we cannot guarantee these will be effective against the native protein due to folding and other conformational concerns. Our service guarantees that the immunized protein (native or recombinant) or peptide will be recognized by the produced antibody.

What assay do you use to confirm the functionality of the produced antibody?

For rabbit polyclonal antibody servies, we guarantee the antibodies will work against the designed peptides in ELISA format. Due to experimental variations, we do not guarantee downstream applications. It is important to understand that the customer takes full responsibility for the peptide designs.

Citations

01	Ko, FC et al. "PKA-induced dimerization of the RhoGAP DLC1 promotes its inhibition of tumorigenesis and metastasis." Nat Commun 4:1618 (2013). PubMed: 23511482