Lentivirus Bundles
Buy more, save more! With the purchase of any lentiviral vector, bundle two or more lentiviral kits listed below to activate additional savings! These specially assembled starter bundles are ideal for beginners new to Lentivirus packaging and transduction, as well as experienced users looking for a great deal.
2nd Generation Lentivirus Bundles
| Product Name | Quantity | Bundle 1 | Bundle 2 Best Value |
Individual Cat. No. | Individual Price | ||
|---|---|---|---|---|---|---|---|
| 2nd Generation Packaging System Mix | 100 μg | ✔ | ✔ | ✔ | LV003 | $285 | |
| DNAfectin™ Plus Transfection Reagent | 1.0 ml | ✔ | ✔ | ✔ | G2500 | $205 | |
| qPCR Lentivirus Titer Kit | 100 rxn | ✔ | ✔ | ✔ | LV900 | $205 | |
| ViralEntry™ Transduction Enhancer | 1.0 ml | ✔ | ✔ | G515 | $185 | ||
| Bundle Cat. No. | LV900-G515 | LV003-G2500 | Lenti-Bundle-1 | Lenti-Bundle-2 | |||
| Bundle Price | $315 | $395 | $485 | $525 | |||
| Bundle Savings | 20% | 20% | 30% | 40% | |||
| Add to Cart | Add to Cart | Add to Cart | Add to Cart |
3rd Generation Lentivirus Bundles
| Product Name | Quantity | Bundle 1 | Bundle 2 Best Value |
Individual Cat. No. | Individual Price | ||
|---|---|---|---|---|---|---|---|
| 3rd Generation Packaging System Mix | 100 μg | ✔ | ✔ | ✔ | LV053 | $345 | |
| DNAfectin™ Plus Transfection Reagent | 1.0 ml | ✔ | ✔ | ✔ | G2500 | $205 | |
| qPCR Lentivirus Titer Kit | 100 rxn | ✔ | ✔ | ✔ | LV900 | $205 | |
| ViralEntry™ Transduction Enhancer | 1.0 ml | ✔ | ✔ | G515 | $185 | ||
| Bundle Cat. No. | LV900-G515 | LV053-G2500 | Lenti-Bundle-3 | Lenti-Bundle-4 | |||
| Bundle Price | $315 | $495 | $525 | $565 | |||
| Bundle Savings | 20% | 10% | 30% | 40% | |||
| Add to Cart | Add to Cart | Add to Cart | Add to Cart |
Which Products do you need?
How do the products fit into the lentivirus packaging workflow?
The Packaging System Mix (1) and the DNAfectinTM Plus Transfection Reagent (2) are required to generate the lentivirus during transfection of the cells with the transfer plasmid. After collection, the virus concentration can be measured (titered) using the qPCR Lentivirus Titer Kit (3). To improve transduction (infection) efficiency, the ViralEntryTM Transduction Enhancer (4) can be added along with the virus particles directly to the cells and media.
2nd Generation vs. 3rd Generation Packaging System Mix
The 2nd Generation Packaging System Mix is recommended unless your facility requires 3rd Generation Packaging Systems for stricter safety regulations.
| Feature | 2nd Generation Packaging System Mix | 3rd Generation Packaging System Mix |
|---|---|---|
| Transfer Plasmid | Can package 2nd or 3rd Generation transfer plasmids (Tat dependent or independent) | Can ONLY package 3rd Generation transfer plasmids (Tat independent) |
| Packaging Plasmids | One plasmid encoding Gag, Pol, Rev, and Tat | Two plasmids: one encoding Gag and Pol, and one encoding Rev |
| Envelope Plasmid | Interchangeable: usually encodes for VSV-G | Interchangeable: usually encodes for VSV-G |
| Safety | Safe. Replication incompetent: Uses 3 separate plasmids encoding various HIV genes. | Safer. Replication incompetent and always self inactivating: Uses 4 plasmids instead of 3 and eliminates the requirement for Tat. |
| Recombinant Virus Yield | Higher yields than 3rd Generation due to fewer plasmids (3) required to be transfected | Lower yields than 2nd Generation due to more plasmids (4) required to be transfected |
Click here to learn more about the Recombinant Lentivirus System.