IHC Optimization Service
If you have a newly developed antibody that has not been tested for IHC staining yet, abm will work with you to provide the best IHC staining conditions for your antibody.
Figure 1. p53 antibody staining yielding high background and low specificity staining.
Figure 2. After IHC optimization service, p53 antibody staining yields low background and high specificity.
Before and After IHC Optimization
For any antibody used for immunohistochemistry staining, optimization of the pretreatment and working concentration is crucial to obtain the best staining results. After fixation of the tissue, proteins are cross linked to one another which prevent some epitopes from binding to the complementary antibody. To improve binding and subsequent staining of the marker of interest, different pretreatment methods are utilized to break the cross linkage of proteins. Antibody concentration will also greatly affect antibody-antigen binding and staining intensity. To obtain the best staining with minimum amount of background, the working concentration for each antibody must be determined experimentally.
Additional Resources:
"We are happy about the CD4 and CD8 IHC staining your lab did. The quality of the IHC staining is very good."
Fugang Zhu, Idera Pharnacueticals IncService Details
Core Services
| Service Name | Description | CAT. NO. | PRICE |
|---|---|---|---|
| IHC Optimization Service |
Optimization of a specific antibody to obtain the best staining results with minimal background. To obtain the best results, 3 methods of pretreatment, and 4 different dilutions will be tested for each case. Deliverables: Images of all 12 conditions tested will be given to the customer. For delivery of actual slides, please inquire. |
HC015 | $375.00/case |
Add-On Services*
Have the tissue, but can’t embed it or cut it? Don’t worry, send us the tissue sample/block, and we can do the embedding and/or cutting for you!
| Add-On Services* | CAT. NO. | PRICE |
|---|---|---|
| Tissue Processing# | HC004 | Inquire |
|
Processing of Formalin-Fixed wet tissue suitable for paraffin embedding. Deliverables: Processed wet tissue suitable for paraffin embedding. |
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| Paraffin Embedding# | HC005 | Inquire |
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Embedding processed tissue into FFPE block suitable for sectioning and subsequent staining. Deliverables: FFPE tissue block. |
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| Cell Pellet Processing and Embedding | HC009 | Inquire |
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Processing of wet cell pellet into FFPE block suitable for sectioning and subsequent staining. Deliverables: Paraffin embedded cell pellet block. |
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| Paraffin Sectioning | HC006 | $5.00/Slide |
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Sectioning of FFPE blocks to produce unstained slides. Unstained slides can be used for H&E, IHC or dye based staining. All slides are cut at 4 μM unless otherwise specified. Please inquire for Tissue Microarray sectioning. Deliverables: Unstained FFPE tissue section slides. |
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| Digital Scan | HC017 | Inquire |
| HC018 | Inquire | |
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Digital scans of stained slides at 20X or 40X magnification. This service is offered in conjunction with IHC, H&E or special staining services. Deliverables: Digital scan will be digitally provided to the customer. |
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#Minimum order of 10 tissue samples is required.
Additional Info
Sample Submission Guidelines and Shipping Instructions
Please fill out our Histology Service Request Form, and email it to quotes@abmgood.com to initiate an inquiry. Before shipping, please fill out our Histology Services Sample Submission Form with your order number and follow the shipping instructions for your samples.
| Specimen type | Shipping instructions |
|---|---|
| Unstained slides | Place slides in microscope slide boxes. Make sure to seal and wrap the box well. |
| Formalin-Fixed wet tissues | Transfer formalin-fixed sample to 70% alcohol. Seal and wrap the container well to prevent leaks. |
| Fixed cell pellet | Transfer the samples to 70% alcohol. Seal and wrap the container well to prevent leaks. |
| FFPE tissue blocks | Ship tissue blocks in a cassette case or bag. During the month of May to August, ship blocks with gel ice pack to prevent the block from melting due to higher temperatures. |
