pLenti-U6-sgRNA-PGK-Neo

Vector Name	pLenti-U6-sgRNA-PGK-Neo
VectorType	Lentiviral Vector
Antibiotic Information	Bacterial: Ampicillin Mammalian: Neomycin/G418
Sequencing Primers	U6 Forward Primer: 5'--GAGGGCCTATTTCCCATGATTCC--3'
Additional Information	No Additional Information

***Note: Features may not be to scale, please refer to sequence or text map for detailed information.

***Note: The vector sequence shown below is representative of the parental vector only and does not include the gene of interest. The sequence of the gene can be found through the accession number link on the product page. The restriction sites within the cloning region may differ from the provided sequence.

1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac 
61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa 
121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat 
181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc 
241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga 
301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg 
361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc 
421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag 
481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc 
541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg 
601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg 
661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac 
721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac 
781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg 
841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg 
901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg 
961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac 
1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg 
1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg 
1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc 
1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc 
1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt 
1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc 
1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact 
1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac 
1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag 
1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg 
1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg 
1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga 
1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt 
1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct 
1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg 
1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt 
1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta 
2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta 
2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt 
2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat 
2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc 
2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg 
2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc 
2401 gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacggg tctctctggt 
2461 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc 
2521 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta 
2581 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa 
2641 cagggacctg aaagcgaaag ggaaaccaga gctctctcga cgcaggactc ggcttgctga 
2701 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag 
2761 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag 
2821 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca 
2881 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac 
2941 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga 
3001 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga 
3061 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac 
3121 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt 
3181 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca 
3241 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt 
3301 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcctcaatg acgctgacgg 
3361 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta 
3421 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa 
3481 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct 
3541 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc 
3601 tggaacagat tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac 
3661 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga 
3721 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct 
3781 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt 
3841 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac 
3901 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga 
3961 gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc ggttaacttt 
4021 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 
4081 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga 
4141 tcacgagact agcctcgagc cccagtggaa agacgcgcag gcaaaacgca ccacgtgacg 
4201 gagcgtgacc gcgcgccgag cgcgcgccaa ggtcgggcag gaagagggcc tatttcccat 
4261 gattccttca tatttgcata tacgatacaa ggctgttaga gagataatta gaattaattt 
4321 gactgtaaac acaaagatat tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg 
4381 gtagtttgca gttttaaaat tatgttttaa aatggactat catatgctta ccgtaacttg 
4441 aaagtatttc gatttcttgg gtttatatat cttgtggaaa ggacgaaaca ccggttttag 
4501 agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa agtggcaccg 
4561 agtcggtgct ttttttctcg agagctagcg aattcccacg gggttggggt tgcgcctttt 
4621 ccaaggcagc cctgggtttg cgcagggacg cggctgctct gggcgtggtt ccgggaaacg 
4681 cagcggcgcc gaccctgggt ctcgcacatt cttcacgtcc gttcgcagcg tcacccggat 
4741 cttcgccgct acccttgtgg gccccccggc gacgcttcct gctccgcccc taagtcggga 
4801 aggttccttg cggttcgcgg cgtgccggac gtgacaaacg gaagccgcac gtctcactag 
4861 taccctcgca gacggacagc gccagggagc aatggcagcg cgccgaccgc gatgggctgt 
4921 ggccaatagc ggctgctcag cagggcgcgc cgagagcagc ggccgggaag gggcggtgcg 
4981 ggaggcgggg tgtggggcgg tagtgtgggc cctgttcctg cccgcgcggt gttccgcatt 
5041 ctgcaagcct ccggagcgca cgtcggcagt cggctccctc gttgaccgaa tcaccgacct 
5101 ctctccccag gggaggaccg gtcgcgatcg ccatggctga acaagatgga ttgcacgcag 
5161 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg 
5221 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca 
5281 agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc 
5341 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg 
5401 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg 
5461 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta 
5521 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag 
5581 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac 
5641 tgttcgccag gctcaaggcg cgcatgcccg acggcgatga tctcgtcgtg acccatggcg 
5701 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg 
5761 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg 
5821 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg 
5881 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagtt taaacgtcga 
5941 caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc 
6001 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg 
6061 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt 
6121 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac 
6181 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc 
6241 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct 
6301 gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct 
6361 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct 
6421 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct 
6481 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggaattcga 
6541 gctcggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa 
6601 aagaaaaggg gggactggaa gggctaattc actcccaacg agacaagatc tgctttttgc 
6661 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg 
6721 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg 
6781 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat 
6841 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg 
6901 aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa 
6961 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc 
7021 caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc 
7081 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag 
7141 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc 
7201 ttttgcgtcg agacgtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg 
7261 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg 
7321 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt 
7381 cccaacagtt gcgcagcctg aatggcgaat ggcgcgacgc gccctgtagc ggcgcattaa 
7441 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc 
7501 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag 
7561 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca 
7621 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc 
7681 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa 
7741 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct 
7801 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa 
7861 cgtttacaat ttcc

                            LOCUS dna 7874 bp 
FEATURES Location/Qualifiers
Promoter 62..90
/gene="amp prom"
Marker 132..992
/gene="amp marker"
ORF 132..992
/sequence="ORF_1 rf(3)"
Rep_Origin 1147..1766
/gene="pBR322 origin"
Promoter 2075..2104
/gene="lac prom"
Promoter 2174..2193
/gene="T3 prom"
Other Gene 2448..2628
/gene="HIV-1_5_LTR other"
Other Gene 2736..2780
/gene="HIV-1_psi_pack other"
misc_binding 3134..3141
/dbxref="REBASE:NotI"
Regulatory_Seq 3290..3523
/gene="RRE reg"
misc_binding 4012..4017
/dbxref="REBASE:HpaI"
misc_binding 4136..4141
/dbxref="REBASE:ClaI"
Promoter 4229..4485
/gene="hU6 prom"
misc_binding 4421..4426
/dbxref="REBASE:NdeI"
ORF 5133..5927
/sequence="ORF_2 rf(3)"
Marker 5138..5924
/gene="NTP_II marker"
misc_binding 5310..5315
/dbxref="REBASE:PstI"
misc_binding 6545..6550
/dbxref="REBASE:KpnI"
Other Gene 6616..6667
/gene="delta_U3 other"
Other Gene 6668..6848
/gene="HIV-1_5_LTR other"
Rep_Origin 7060..7137
/gene="SV40 origin"
misc_binding 7140..7152
/dbxref="REBASE:SfiI"
misc_binding 7191..7196
/dbxref="REBASE:StuI"
Promoter 7228..7246
/gene="T7 prom"
Reporter 7254..7412
/gene="lacZ_a reporter"
Rep_Origin 7434..7740
/gene="f1 origin"
BASE COUNT 1955 a 1912 c 2074 g 1933 t 0 others

***Note: The text map shown below is representative of the parental vector only and does not include the gene of interest. The sequence of the gene can be found through the accession number link on the product page.

1 caggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatac 60
GTCCACCGTGAAAAGCCCCTTTACACGCGCCTTGGGGATAAACAAATAAAAAGATTTATG

amp prom(62,90)>>> 
|
61 attcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaa 120
TAAGTTTATACATAGGCGAGTACTCTGTTATTGGGACTATTTACGAAGTTATTATAACTT

ORF_1 rf(3)(132,992)>>> 
|
amp marker(132,992)>>> 
|
121 aaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcat 180
TTTCCTTCTCATACTCATAAGTTGTAAAGGCACAGCGGGAATAAGGGAAAAAACGCCGTA

181 tttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatc 240
AAACGGAAGGACAAAAACGAGTGGGTCTTTGCGACCACTTTCATTTTCTACGACTTCTAG

241 agttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgaga 300
TCAACCCACGTGCTCACCCAATGTAGCTTGACCTAGAGTTGTCGCCATTCTAGGAACTCT

301 gttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcg 360
CAAAAGCGGGGCTTCTTGCAAAAGGTTACTACTCGTGAAAATTTCAAGACGATACACCGC

361 cggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctc 420
GCCATAATAGGGCATAACTGCGGCCCGTTCTCGTTGAGCCAGCGGCGTATGTGATAAGAG

421 agaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacag 480
TCTTACTGAACCAACTCATGAGTGGTCAGTGTCTTTTCGTAGAATGCCTACCGTACTGTC

481 taagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttc 540
ATTCTCTTAATACGTCACGACGGTATTGGTACTCACTATTGTGACGCCGGTTGAATGAAG

541 tgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatg 600
ACTGTTGCTAGCCTCCTGGCTTCCTCGATTGGCGAAAAAACGTGTTGTACCCCCTAGTAC

601 taactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtg 660
ATTGAGCGGAACTAGCAACCCTTGGCCTCGACTTACTTCGGTATGGTTTGCTGCTCGCAC

661 acaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactac 720
TGTGGTGCTACGGACATCGTTACCGTTGTTGCAACGCGTTTGATAATTGACCGCTTGATG

721 ttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggac 780
AATGAGATCGAAGGGCCGTTGTTAATTATCTGACCTACCTCCGCCTATTTCAACGTCCTG

781 cacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtg 840
GTGAAGACGCGAGCCGGGAAGGCCGACCGACCAAATAACGACTATTTAGACCTCGGCCAC

841 agcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcg 900
TCGCACCCAGAGCGCCATAGTAACGTCGTGACCCCGGTCTACCATTCGGGAGGGCATAGC

901 tagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctg 960
ATCAATAGATGTGCTGCCCCTCAGTCCGTTGATACCTACTTGCTTTATCTGTCTAGCGAC

961 agataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatac 1020
TCTATCCACGGAGTGACTAATTCGTAACCATTGACAGTCTGGTTCAAATGAGTATATATG

1021 tttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttg 1080
AAATCTAACTAAATTTTGAAGTAAAAATTAAATTTTCCTAGATCCACTTCTAGGAAAAAC

1081 ataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccg 1140
TATTAGAGTACTGGTTTTAGGGAATTGCACTCAAAAGCAAGGTGACTCGCAGTCTGGGGC

pBR322 origin(1147,1766)>>> 
|
1141 tagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgc 1200
ATCTTTTCTAGTTTCCTAGAAGAACTCTAGGAAAAAAAGACGCGCATTAGACGACGAACG

1201 aaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactc 1260
TTTGTTTTTTTGGTGGCGATGGTCGCCACCAAACAAACGGCCTAGTTCTCGATGGTTGAG

1261 tttttccgaaggtaactggcttcagcagagcgcagataccaaatactgtccttctagtgt 1320
AAAAAGGCTTCCATTGACCGAAGTCGTCTCGCGTCTATGGTTTATGACAGGAAGATCACA

1321 agccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgc 1380
TCGGCATCAATCCGGTGGTGAAGTTCTTGAGACATCGTGGCGGATGTATGGAGCGAGACG

1381 taatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggact 1440
ATTAGGACAATGGTCACCGACGACGGTCACCGCTATTCAGCACAGAATGGCCCAACCTGA

1441 caagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacac 1500
GTTCTGCTATCAATGGCCTATTCCGCGTCGCCAGCCCGACTTGCCCCCCAAGCACGTGTG

1501 agcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgag 1560
TCGGGTCGAACCTCGCTTGCTGGATGTGGCTTGACTCTATGGATGTCGCACTCGATACTC

1561 aaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcg 1620
TTTCGCGGTGCGAAGGGCTTCCCTCTTTCCGCCTGTCCATAGGCCATTCGCCGTCCCAGC

1621 gaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctg 1680
CTTGTCCTCTCGCGTGCTCCCTCGAAGGTCCCCCTTTGCGGACCATAGAAATATCAGGAC

1681 tcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcgga 1740
AGCCCAAAGCGGTGGAGACTGAACTCGCAGCTAAAAACACTACGAGCAGTCCCCCCGCCT

1741 gcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggcctt 1800
CGGATACCTTTTTGCGGTCGTTGCGCCGGAAAAATGCCAAGGACCGGAAAACGACCGGAA

1801 ttgctcacatgttctttcctgcgttatcccctgattctgtggataaccgtattaccgcct 1860
AACGAGTGTACAAGAAAGGACGCAATAGGGGACTAAGACACCTATTGGCATAATGGCGGA

1861 ttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcg 1920
AACTCACTCGACTATGGCGAGCGGCGTCGGCTTGCTGGCTCGCGTCGCTCAGTCACTCGC

1921 aggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcatt 1980
TCCTTCGCCTTCTCGCGGGTTATGCGTTTGGCGGAGAGGGGCGCGCAACCGGCTAAGTAA

1981 aatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaatta 2040
TTACGTCGACCGTGCTGTCCAAAGGGCTGACCTTTCGCCCGTCACTCGCGTTGCGTTAAT

lac prom(2075,2104)>>> 
|
2041 atgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgta 2100
TACACTCAATCGAGTGAGTAATCCGTGGGGTCCGAAATGTGAAATACGAAGGCCGAGCAT

2101 tgttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgatt 2160
ACAACACACCTTAACACTCGCCTATTGTTAAAGTGTGTCCTTTGTCGATACTGGTACTAA

T3 prom(2174,2193)>>> 
|
2161 acgccaagcgcgcaattaaccctcactaaagggaacaaaagctggagctgcaagcttaat 2220
TGCGGTTCGCGCGTTAATTGGGAGTGATTTCCCTTGTTTTCGACCTCGACGTTCGAATTA

2221 gtagtcttatgcaatactcttgtagtcttgcaacatggtaacgatgagttagcaacatgc 2280
CATCAGAATACGTTATGAGAACATCAGAACGTTGTACCATTGCTACTCAATCGTTGTACG

2281 cttacaaggagagaaaaagcaccgtgcatgccgattggtggaagtaaggtggtacgatcg 2340
GAATGTTCCTCTCTTTTTCGTGGCACGTACGGCTAACCACCTTCATTCCACCATGCTAGC

2341 tgccttattaggaaggcaacagacgggtctgacatggattggacgaaccactgaattgcc 2400
ACGGAATAATCCTTCCGTTGTCTGCCCAGACTGTACCTAACCTGCTTGGTGACTTAACGG

HIV-1_5_LTR other(2448,2628)>>> 
|
2401 gcattgcagagatattgtatttaagtgcctagctcgatacaataaacgggtctctctggt 2460
CGTAACGTCTCTATAACATAAATTCACGGATCGAGCTATGTTATTTGCCCAGAGAGACCA

2461 tagaccagatctgagcctgggagctctctggctaactagggaacccactgcttaagcctc 2520
ATCTGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAATTCGGAG

2521 aataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggta 2580
TTATTTCGAACGGAACTCACGAAGTTCATCACACACGGGCAGACAACACACTGAGACCAT

2581 actagagatccctcagacccttttagtcagtgtggaaaatctctagcagtggcgcccgaa 2640
TGATCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCACCGCGGGCTT

2641 cagggacctgaaagcgaaagggaaaccagagctctctcgacgcaggactcggcttgctga 2700
GTCCCTGGACTTTCGCTTTCCCTTTGGTCTCGAGAGAGCTGCGTCCTGAGCCGAACGACT

HIV-1_psi_pack other(2736,2780)>>> 
|
2701 agcgcgcacggcaagaggcgaggggcggcgactggtgagtacgccaaaaattttgactag 2760
TCGCGCGTGCCGTTCTCCGCTCCCCGCCGCTGACCACTCATGCGGTTTTTAAAACTGATC

2761 cggaggctagaaggagagagatgggtgcgagagcgtcagtattaagcgggggagaattag 2820
GCCTCCGATCTTCCTCTCTCTACCCACGCTCTCGCAGTCATAATTCGCCCCCTCTTAATC

2821 atcgcgatgggaaaaaattcggttaaggccagggggaaagaaaaaatataaattaaaaca 2880
TAGCGCTACCCTTTTTTAAGCCAATTCCGGTCCCCCTTTCTTTTTTATATTTAATTTTGT

2881 tatagtatgggcaagcagggagctagaacgattcgcagttaatcctggcctgttagaaac 2940
ATATCATACCCGTTCGTCCCTCGATCTTGCTAAGCGTCAATTAGGACCGGACAATCTTTG

2941 atcagaaggctgtagacaaatactgggacagctacaaccatcccttcagacaggatcaga 3000
TAGTCTTCCGACATCTGTTTATGACCCTGTCGATGTTGGTAGGGAAGTCTGTCCTAGTCT

3001 agaacttagatcattatataatacagtagcaaccctctattgtgtgcatcaaaggataga 3060
TCTTGAATCTAGTAATATATTATGTCATCGTTGGGAGATAACACACGTAGTTTCCTATCT

3061 gataaaagacaccaaggaagctttagacaagatagaggaagagcaaaacaaaagtaagac 3120
CTATTTTCTGTGGTTCCTTCGAAATCTGTTCTATCTCCTTCTCGTTTTGTTTTCATTCTG

NotI 
|
3121 caccgcacagcaagcggccgctgatcttcagacctggaggaggagatatgagggacaatt 3180
GTGGCGTGTCGTTCGCCGGCGACTAGAAGTCTGGACCTCCTCCTCTATACTCCCTGTTAA

3181 ggagaagtgaattatataaatataaagtagtaaaaattgaaccattaggagtagcaccca 3240
CCTCTTCACTTAATATATTTATATTTCATCATTTTTAACTTGGTAATCCTCATCGTGGGT

RRE reg(3290,3523)>>> 
|
3241 ccaaggcaaagagaagagtggtgcagagagaaaaaagagcagtgggaataggagctttgt 3300
GGTTCCGTTTCTCTTCTCACCACGTCTCTCTTTTTTCTCGTCACCCTTATCCTCGAAACA

3301 tccttgggttcttgggagcagcaggaagcactatgggcgcagcctcaatgacgctgacgg 3360
AGGAACCCAAGAACCCTCGTCGTCCTTCGTGATACCCGCGTCGGAGTTACTGCGACTGCC

3361 tacaggccagacaattattgtctggtatagtgcagcagcagaacaatttgctgagggcta 3420
ATGTCCGGTCTGTTAATAACAGACCATATCACGTCGTCGTCTTGTTAAACGACTCCCGAT

3421 ttgaggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctccaggcaa 3480
AACTCCGCGTTGTCGTAGACAACGTTGAGTGTCAGACCCCGTAGTTCGTCGAGGTCCGTT

3481 gaatcctggctgtggaaagatacctaaaggatcaacagctcctggggatttggggttgct 3540
CTTAGGACCGACACCTTTCTATGGATTTCCTAGTTGTCGAGGACCCCTAAACCCCAACGA

3541 ctggaaaactcatttgcaccactgctgtgccttggaatgctagttggagtaataaatctc 3600
GACCTTTTGAGTAAACGTGGTGACGACACGGAACCTTACGATCAACCTCATTATTTAGAG

3601 tggaacagattggaatcacacgacctggatggagtgggacagagaaattaacaattacac 3660
ACCTTGTCTAACCTTAGTGTGCTGGACCTACCTCACCCTGTCTCTTTAATTGTTAATGTG

3661 aagcttaatacactccttaattgaagaatcgcaaaaccagcaagaaaagaatgaacaaga 3720
TTCGAATTATGTGAGGAATTAACTTCTTAGCGTTTTGGTCGTTCTTTTCTTACTTGTTCT

3721 attattggaattagataaatgggcaagtttgtggaattggtttaacataacaaattggct 3780
TAATAACCTTAATCTATTTACCCGTTCAAACACCTTAACCAAATTGTATTGTTTAACCGA

3781 gtggtatataaaattattcataatgatagtaggaggcttggtaggtttaagaatagtttt 3840
CACCATATATTTTAATAAGTATTACTATCATCCTCCGAACCATCCAAATTCTTATCAAAA

3841 tgctgtactttctatagtgaatagagttaggcagggatattcaccattatcgtttcagac 3900
ACGACATGAAAGATATCACTTATCTCAATCCGTCCCTATAAGTGGTAATAGCAAAGTCTG

3901 ccacctcccaaccccgaggggacccgacaggcccgaaggaatagaagaagaaggtggaga 3960
GGTGGAGGGTTGGGGCTCCCCTGGGCTGTCCGGGCTTCCTTATCTTCTTCTTCCACCTCT

HpaI 
|
3961 gagagacagagacagatccattcgattagtgaacggatctcgacggtatcggttaacttt 4020
CTCTCTGTCTCTGTCTAGGTAAGCTAATCACTTGCCTAGAGCTGCCATAGCCAATTGAAA

4021 taaaagaaaaggggggattggggggtacagtgcaggggaaagaatagtagacataatagc 4080
ATTTTCTTTTCCCCCCTAACCCCCCATGTCACGTCCCCTTTCTTATCATCTGTATTATCG

ClaI 
|
4081 aacagacatacaaactaaagaattacaaaaacaaattacaaaaattcaaaattttATcga 4140
TTGTCTGTATGTTTGATTTCTTAATGTTTTTGTTTAATGTTTTTAAGTTTTAAAATAGCT

4141 tcacgagactagcCTCGAGCCCCAGTGGAAAGACGCGCAGGCAAAACGCACCACGTGACG 4200
AGTGCTCTGATCGGAGCTCGGGGTCACCTTTCTGCGCGTCCGTTTTGCGTGGTGCACTGC

hU6 prom(4229,4485)>>> 
|
4201 GAGCGTGACCGCGCGCCGAGCGCGCGCCAAGGTCGGGCAGGAAGAGGGCCTATTTCCCAT 4260
CTCGCACTGGCGCGCGGCTCGCGCGCGGTTCCAGCCCGTCCTTCTCCCGGATAAAGGGTA

4261 GATTCCTTCATATTTGCATATACGATACAAGGCTGTTAGAGAGATAATTAGAATTAATTT 4320
CTAAGGAAGTATAAACGTATATGCTATGTTCCGACAATCTCTCTATTAATCTTAATTAAA

4321 GACTGTAAACACAAAGATATTAGTACAAAATACGTGACGTAGAAAGTAATAATTTCTTGG 4380
CTGACATTTGTGTTTCTATAATCATGTTTTATGCACTGCATCTTTCATTATTAAAGAACC

NdeI 
|
4381 GTAGTTTGCAGTTTTAAAATTATGTTTTAAAATGGACTATCATATGCTTACCGTAACTTG 4440
CATCAAACGTCAAAATTTTAATACAAAATTTTACCTGATAGTATACGAATGGCATTGAAC

4441 AAAGTATTTCGATTTCTTGGGTTTATATATCTTGTGGAAAGGACGAAACACCGGttttag 4500
TTTCATAAAGCTAAAGAACCCAAATATATAGAACACCTTTCCTGCTTTGTGGCCAAAATC

4501 agctagaaatagcaagttaaaataaggctagtccgttatcaacttgaaaaagtggcaccg 4560
TCGATCTTTATCGTTCAATTTTATTCCGATCAGGCAATAGTTGAACTTTTTCACCGTGGC

4561 agtcggtgcTTTTTTTctcgagAGCTAGCGAATTCCCACGGGGTTGGGGTTGCGCCTTTT 4620
TCAGCCACGAAAAAAAGAGCTCTCGATCGCTTAAGGGTGCCCCAACCCCAACGCGGAAAA

4621 CCAAGGCAGCCCTGGGTTTGCGCAGGGACGCGGCTGCTCTGGGCGTGGTTCCGGGAAACG 4680
GGTTCCGTCGGGACCCAAACGCGTCCCTGCGCCGACGAGACCCGCACCAAGGCCCTTTGC

4681 CAGCGGCGCCGACCCTGGGTCTCGCACATTCTTCACGTCCGTTCGCAGCGTCACCCGGAT 4740
GTCGCCGCGGCTGGGACCCAGAGCGTGTAAGAAGTGCAGGCAAGCGTCGCAGTGGGCCTA

4741 CTTCGCCGCTACCCTTGTGGGCCCCCCGGCGACGCTTCCTGCTCCGCCCCTAAGTCGGGA 4800
GAAGCGGCGATGGGAACACCCGGGGGGCCGCTGCGAAGGACGAGGCGGGGATTCAGCCCT

4801 AGGTTCCTTGCGGTTCGCGGCGTGCCGGACGTGACAAACGGAAGCCGCACGTCTCACTAG 4860
TCCAAGGAACGCCAAGCGCCGCACGGCCTGCACTGTTTGCCTTCGGCGTGCAGAGTGATC

4861 TACCCTCGCAGACGGACAGCGCCAGGGAGCAATGGCAGCGCGCCGACCGCGATGGGCTGT 4920
ATGGGAGCGTCTGCCTGTCGCGGTCCCTCGTTACCGTCGCGCGGCTGGCGCTACCCGACA

4921 GGCCAATAGCGGCTGCTCAGCGGGGCGCGCCGAGAGCAGCGGCCGGGAAGGGGCGGTGCG 4980
CCGGTTATCGCCGACGAGTCGCCCCGCGCGGCTCTCGTCGCCGGCCCTTCCCCGCCACGC

4981 GGAGGCGGGGTGTGGGGCGGTAGTGTGGGCCCTGTTCCTGCCCGCGCGGTGTTCCGCATT 5040
CCTCCGCCCCACACCCCGCCATCACACCCGGGACAAGGACGGGCGCGCCACAAGGCGTAA

5041 CTGCAAGCCTCCGGAGCGCACGTCGGCAGTCGGCTCCCTCGTTGACCGAATCACCGACCT 5100
GACGTTCGGAGGCCTCGCGTGCAGCCGTCAGCCGAGGGAGCAACTGGCTTAGTGGCTGGA

NTP_II marker(5138,5924)>>> 
|
ORF_2 rf(3)(5133,5927)>>> 
| |
5101 CTCTCCCCAGGGGAGGACCGGTCGCGATCGCCAtggctgaacaagatggattgcacgcag 5160
GAGAGGGGTCCCCTCCTGGCCAGCGCTAGCGGTACCGACTTGTTCTACCTAACGTGCGTC

5161 gttctccggccgcttgggtggagaggctattcggctatgactgggcacaacagacaatcg 5220
CAAGAGGCCGGCGAACCCACCTCTCCGATAAGCCGATACTGACCCGTGTTGTCTGTTAGC

5221 gctgctctgatgccgccgtgttccggctgtcagcgcaggggcgcccggttctttttgtca 5280
CGACGAGACTACGGCGGCACAAGGCCGACAGTCGCGTCCCCGCGGGCCAAGAAAAACAGT

PstI 
|
5281 agaccgacctgtccggtgccctgaatgaactgcaggacgaggcagcgcggctatcgtggc 5340
TCTGGCTGGACAGGCCACGGGACTTACTTGACGTCCTGCTCCGTCGCGCCGATAGCACCG

5341 tggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactgaagcgggaaggg 5400
ACCGGTGCTGCCCGCAAGGAACGCGTCGACACGAGCTGCAACAGTGACTTCGCCCTTCCC

5401 actggctgctattgggcgaagtgccggggcaggatctcctgtcatctcaccttgctcctg 5460
TGACCGACGATAACCCGCTTCACGGCCCCGTCCTAGAGGACAGTAGAGTGGAACGAGGAC

5461 ccgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttgatccggcta 5520
GGCTCTTTCATAGGTAGTACCGACTACGTTACGCCGCCGACGTATGCGAACTAGGCCGAT

5521 cctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaag 5580
GGACGGGTAAGCTGGTGGTTCGCTTTGTAGCGTAGCTCGCTCGTGCATGAGCCTACCTTC

5581 ccggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgccagccgaac 5640
GGCCAGAACAGCTAGTCCTACTAGACCTGCTTCTCGTAGTCCCCGAGCGCGGTCGGCTTG

5641 tgttcgccaggctcaaggcgcgcatgcccgacggcgatgatctcgtcgtgacccatggcg 5700
ACAAGCGGTCCGAGTTCCGCGCGTACGGGCTGCCGCTACTAGAGCAGCACTGGGTACCGC

5701 atgcctgcttgccgaatatcatggtggaaaatggccgcttttctggattcatcgactgtg 5760
TACGGACGAACGGCTTATAGTACCACCTTTTACCGGCGAAAAGACCTAAGTAGCTGACAC

5761 gccggctgggtgtggcggaccgctatcaggacatagcgttggctacccgtgatattgctg 5820
CGGCCGACCCACACCGCCTGGCGATAGTCCTGTATCGCAACCGATGGGCACTATAACGAC

5821 aagagcttggcggcgaatgggctgaccgcttcctcgtgctttacggtatcgccgctcccg 5880
TTCTCGAACCGCCGCTTACCCGACTGGCGAAGGAGCACGAAATGCCATAGCGGCGAGGGC

5881 attcgcagcgcatcgccttctatcgccttcttgacgagttcttctgaGTTTAAACGtcga 5940
TAAGCGTCGCGTAGCGGAAGATAGCGGAAGAACTGCTCAAGAAGACTCAAATTTGCAGCT

5941 caatcaacctctggattacaaaatttgtgaaagattgactggtattcttaactatgttgc 6000
GTTAGTTGGAGACCTAATGTTTTAAACACTTTCTAACTGACCATAAGAATTGATACAACG

6001 tccttttacgctatgtggatacgctgctttaatgcctttgtatcatgctattgcttcccg 6060
AGGAAAATGCGATACACCTATGCGACGAAATTACGGAAACATAGTACGATAACGAAGGGC

6061 tatggctttcattttctcctccttgtataaatcctggttgctgtctctttatgaggagtt 6120
ATACCGAAAGTAAAAGAGGAGGAACATATTTAGGACCAACGACAGAGAAATACTCCTCAA

6121 gtggcccgttgtcaggcaacgtggcgtggtgtgcactgtgtttgctgacgcaacccccac 6180
CACCGGGCAACAGTCCGTTGCACCGCACCACACGTGACACAAACGACTGCGTTGGGGGTG

6181 tggttggggcattgccaccacctgtcagctcctttccgggactttcgctttccccctccc 6240
ACCAACCCCGTAACGGTGGTGGACAGTCGAGGAAAGGCCCTGAAAGCGAAAGGGGGAGGG

6241 tattgccacggcggaactcatcgccgcctgccttgcccgctgctggacaggggctcggct 6300
ATAACGGTGCCGCCTTGAGTAGCGGCGGACGGAACGGGCGACGACCTGTCCCCGAGCCGA

6301 gttgggcactgacaattccgtggtgttgtcggggaagctgacgtcctttccatggctgct 6360
CAACCCGTGACTGTTAAGGCACCACAACAGCCCCTTCGACTGCAGGAAAGGTACCGACGA

6361 cgcctgtgttgccacctggattctgcgcgggacgtccttctgctacgtcccttcggccct 6420
GCGGACACAACGGTGGACCTAAGACGCGCCCTGCAGGAAGACGATGCAGGGAAGCCGGGA

6421 caatccagcggaccttccttcccgcggcctgctgccggctctgcggcctcttccgcgtct 6480
GTTAGGTCGCCTGGAAGGAAGGGCGCCGGACGACGGCCGAGACGCCGGAGAAGGCGCAGA

6481 cgccttcgccctcagacgagtcggatctccctttgggccgcctccccgcctggaattcga 6540
GCGGAAGCGGGAGTCTGCTCAGCCTAGAGGGAAACCCGGCGGAGGGGCGGACCTTAAGCT

KpnI 
|
6541 gctcggtacctttaagaccaatgacttacaaggcagctgtagatcttagccactttttaa 6600
CGAGCCATGGAAATTCTGGTTACTGAATGTTCCGTCGACATCTAGAATCGGTGAAAAATT

delta_U3 other(6616,6667)>>> 
|
6601 aagaaaaggggggactggaagggctaattcactcccaacgagacaagatctgctttttgc 6660
TTCTTTTCCCCCCTGACCTTCCCGATTAAGTGAGGGTTGCTCTGTTCTAGACGAAAAACG

HIV-1_5_LTR other(6668,6848)>>> 
|
6661 ttgtactgggtctctctggttagaccagatctgagcctgggagctctctggctaactagg 6720
AACATGACCCAGAGAGACCAATCTGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCC

6721 gaacccactgcttaagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccg 6780
CTTGGGTGACGAATTCGGAGTTATTTCGAACGGAACTCACGAAGTTCATCACACACGGGC

6781 tctgttgtgtgactctggtaactagagatccctcagacccttttagtcagtgtggaaaat 6840
AGACAACACACTGAGACCATTGATCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTA

6841 ctctagcagtagtagttcatgtcatcttattattcagtatttataacttgcaaagaaatg 6900
GAGATCGTCATCATCAAGTACAGTAGAATAATAAGTCATAAATATTGAACGTTTCTTTAC

6901 aatatcagagagtgagaggaacttgtttattgcagcttataatggttacaaataaagcaa 6960
TTATAGTCTCTCACTCTCCTTGAACAAATAACGTCGAATATTACCAATGTTTATTTCGTT

6961 tagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtc 7020
ATCGTAGTGTTTAAAGTGTTTATTTCGTAAAAAAAGTGACGTAAGATCAACACCAAACAG

SV40 origin(7060,7137)>>> 
|
7021 caaactcatcaatgtatcttatcatgtctggctctagctatcccgcccctaactccgccc 7080
GTTTGAGTAGTTACATAGAATAGTACAGACCGAGATCGATAGGGCGGGGATTGAGGCGGG

7081 agttccgcccattctccgccccatggctgactaattttttttatttatgcagaggccgag 7140
TCAAGGCGGGTAAGAGGCGGGGTACCGACTGATTAAAAAAAATAAATACGTCTCCGGCTC

SfiI StuI 
| |
7141 gccgcctcggcctctgagctattccagaagtagtgaggaggcttttttggaggcctaggc 7200
CGGCGGAGCCGGAGACTCGATAAGGTCTTCATCACTCCTCCGAAAAAACCTCCGGATCCG

T7 prom(7228,7246)<<< lacZ_a reporter(7254,7412)>>> 
| |
7201 ttttgcgtcgagacgtacccaattcgccctatagtgagtcgtattacgcgcgctcactgg 7260
AAAACGCAGCTCTGCATGGGTTAAGCGGGATATCACTCAGCATAATGCGCGCGAGTGACC

7261 ccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttg 7320
GGCAGCAAAATGTTGCAGCACTGACCCTTTTGGGACCGCAATGGGTTGAATTAGCGGAAC

7321 cagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgccctt 7380
GTCGTGTAGGGGGAAAGCGGTCGACCGCATTATCGCTTCTCCGGGCGTGGCTAGCGGGAA

f1 origin(7434,7740)>>> 
|
7381 cccaacagttgcgcagcctgaatggcgaatggcgcgacgcgccctgtagcggcgcattaa 7440
GGGTTGTCAACGCGTCGGACTTACCGCTTACCGCGCTGCGCGGGACATCGCCGCGTAATT

7441 gcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgc 7500
CGCGCCGCCCACACCACCAATGCGCGTCGCACTGGCGATGTGAACGGTCGCGGGATCGCG

7501 ccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaag 7560
GGCGAGGAAAGCGAAAGAAGGGAAGGAAAGAGCGGTGCAAGCGGCCGAAAGGGGCAGTTC

7561 ctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgacccca 7620
GAGATTTAGCCCCCGAGGGAAATCCCAAGGCTAAATCACGAAATGCCGTGGAGCTGGGGT

7621 aaaaacttgattagggtgatggttcacgtagtgggccatcgccctgatagacggtttttc 7680
TTTTTGAACTAATCCCACTACCAAGTGCATCACCCGGTAGCGGGACTATCTGCCAAAAAG

7681 gccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaa 7740
CGGGAAACTGCAACCTCAGGTGCAAGAAATTATCACCTGAGAACAAGGTTTGACCTTGTT

7741 cactcaaccctatctcggtctattcttttgatttataagggattttgccgatttcggcct 7800
GTGAGTTGGGATAGAGCCAGATAAGAAAACTAAATATTCCCTAAAACGGCTAAAGCCGGA

7801 attggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaa 7860
TAACCAATTTTTTACTCGACTAAATTGTTTTTAAATTGCGCTTAAAATTGTTTTATAATT

7861 cgtttacaatttcc 7874
GCAAATGTTAAAGG