pRetroG-CMV-HA
| Vector Name | pRetroG-CMV-HA |
| VectorType | Retroviral Plasmid |
| Antibiotic Information | Bacterial: Ampicillin Mammalian: Puromycin |
| Sequencing Primers | CMV sequencing primer 5`---CGC AAA TGG GCG GTA GGC GTG---3` |
| Additional Information | The gene expression is driven by a CMV promoter, with a C-terminal HA tag. Original Vector: 8026bp Vector without Insert Cassette: 6321bp |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 tttgaaagac cccacccgta ggtggcaagc tagcttaagt aacgccactt tgcaaggcat 61 ggaaaaatac ataactgaga atagaaaagt tcagatcaag gtcaggaaca aagaaacagc 121 tgaataccaa acaggatatc tgtggtaagc ggttcctgcc ccggctcagg gccaagaaca 181 gatgagacag ctgagtgatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg 241 ctcggggcca agaacagatg gtccccagat gcggtccagc cctcagcagt ttctagtgaa 301 tcatcagatg tttccagggt gccccaagga cctgaaaatg accctgtacc ttatttgaac 361 taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc cgctctccga gctcaataaa 421 agagcccaca acccctcact cggcgcgcca gtcttccgat agactgcgtc gcccgggtac 481 ccgtattccc aataaagcct cttgctgttt gcatccgaat cgtggtctcg ctgttccttg 541 ggagggtctc ctctgagtga ttgactaccc acgacggggg tctttcattt gggggctcgt 601 ccgggatttg gagacccctg cccagggacc accgacccac caccgggagg taagctggcc 661 agcaacttat ctgtgtctgt ccgattgtct agtgtctatg tttgatgtta tgcgcctgcg 721 tctgtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa ctgacgagtt 781 ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc gtttttgtgg 841 cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg tggttctggt 901 aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt cggtttggaa 961 ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct 1021 gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc ccttaagttt 1081 gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa 1141 gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg tcggatggcc 1201 gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg tcttttcacc 1261 tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag ccttggcttt 1321 tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc ctcttcctcc 1381 atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta 1441 tccagccctc actccttctc taggcgccgg aattgatccc ccgggctgca ggtcggccgc 1501 cacgaccggt gccgccacca tcccctgacc cacgcccctg acccctcaca aggagacgac 1561 cttccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtcccccggg 1621 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgacc 1681 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc 1741 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc 1801 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga 1861 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca 1921 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc 1981 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct 2041 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca 2101 ccgccgacgt cgagtgcccg aaggaccgcg cgacctggtg catgacccgc aagcccggtg 2161 cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga ccccatggct 2221 ccgaccgaag ccgacccggg cggccccgcc gaccccgcac ccgcccccga ggcccaccga 2281 ctctagtaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc 2341 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg 2401 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa 2461 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca 2521 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac 2581 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc 2641 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga 2701 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg 2761 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta 2821 cggtgggagg tctatataag cagagctggt ttagtgaacc gtcagatccg ctagcgctac 2881 cggactcaga tctaagcttc ctagcgatat caacaagttt gtacaaaaaa gctgaacgag 2941 aaacgtaaaa tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta 3001 cataatactg taaaacacaa catatccagt cactatggcg gccgcattag gcaccccagg 3061 ctttacactt tatgcttccg gctcgtataa tgtgtggatt ttgagttagg atccgtcgag 3121 attttcagga gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga 3181 tatatcccaa tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac 3241 ctataaccag accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa 3301 gcacaagttt tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga 3361 attccgtatg gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta 3421 caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga 3481 tttccggcag tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc 3541 ctatttccct aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag 3601 tttcaccagt tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac 3661 catgggcaaa tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca 3721 tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg 3781 cgatgagtgg cagggcgggg cgtaaagatc tggatccggc ttactaaaag ccagataaca 3841 gtatgcgtat ttgcgcgctg atttttgcgg tataagaata tatactgata tgtatacccg 3901 aagtatgtca aaaagaggta tgctatgaag cagcgtatta cagtgacagt tgacagcgac 3961 agctatcagt tgctcaaggc atatatgatg tcaatatctc cggtctggta agcacaacca 4021 tgcagaatga agcccgtcgt ctgcgtgccg aacgctggaa agcggaaaat caggaaggga 4081 tggctgaggt cgcccggttt attgaaatga acggctcttt tgctgacgag aacaggggct 4141 ggtgaaatgc agtttaaggt ttacacctat aaaagagaga gccgttatcg tctgtttgtg 4201 gatgtacaga gtgatattat tgacacgccc gggcgacgga tggtgatccc cctggccagt 4261 gcacgtctgc tgtcagataa agtctcccgt gaactttacc cggtggtgca tatcggggat 4321 gaaagctggc gcatgatgac caccgatatg gccagtgtgc cggtctccgt tatcggggaa 4381 gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc 4441 tggggaatat aaatgtcagg ctcccttata cacagccagt ctgcaggtcg accatagtga 4501 ctggatatgt tgtgttttac agtattatgt agtctgtttt ttatgcaaaa tctaatttaa 4561 tatattgata tttatatcat tttacgtttc tcgttcagct ttcttgtaca aagtggttga 4621 tatcctcgag tacccatacg acgtcccaga ctacgcttga gtttaaacga attcgttagg 4681 ccattaaggc ctgtcgacaa gcggccgcct cggccaaaca tcgataaaat aaaagatttt 4741 atttagtctc cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg gcaagctagc 4801 ttaagtaacg ccattttgca aggcatggaa aaatacataa ctgagaatag agaagttcag 4861 atcaaggtca ggaacagatg gaacagctga atatgggcca aacaggatat ctgtggtaag 4921 cagttcctgc cccggctcag ggccaagaac agatggaaca gctgaatatg ggccaaacag 4981 gatatctgtg gtaagcagtt cctgccccgg ctcagggcca agaacagatg gtccccagat 5041 gcggtccagc cctcagcagt ttctagagaa ccatcagatg tttccagggt gccccaagga 5101 cctgaaatga ccctgtgcct tatttgaact aaccaatcag ttcgcttctc gcttctgttc 5161 gcgcgcttct gctccccgag ctcaataaaa gagcccacaa cccctcactc ggggcgccag 5221 tcctccgatt gactgagtcg cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc 5281 atccgacttg tggtctcgct gttccttggg agggtctcct ctgagtgatt gactacccgt 5341 cagcgggggt ctttcatttg ggggctcgtc cgggatcggg agacccctgc ccagggacca 5401 ccgacccacc accgggaggt aagctggctg cctcgcgcgt ttcggtgatg acggtgaaaa 5461 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag 5521 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac 5581 ccagtcacgt agcgatagcg gagtgtactg ataacttcgt ataatgtatg ctatacgaag 5641 ttattaggtc tgaagaggag tttacgtcca gccaagctag ggccgcgatc cggaaccctt 5701 aatataactt cgtataatgt atgctatacg aagttatcag tactggctta actatgcggc 5761 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt 5821 aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc 5881 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 5941 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 6001 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca 6061 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 6121 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 6181 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta 6241 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 6301 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 6361 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 6421 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg 6481 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 6541 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag 6601 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa 6661 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat 6721 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc 6781 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc 6841 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc 6901 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc 6961 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc 7021 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt 7081 gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc 7141 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa 7201 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt 7261 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg 7321 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc 7381 gagttgctct tgcccggcgt caacacggga taataccgcg ccacatagca gaactttaaa 7441 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt 7501 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt 7561 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag 7621 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta 7681 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat 7741 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat 7801 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtcttc aagaattgct 7861 agcaattgct agcaattgct agcaattcat accagatcac cgaaaactgt cctccaaatg 7921 tgtccccctc acactcccaa attcgcgggc ttctgcctct tagaccactc taccctattc 7981 cccacactca ccggagccaa agccgcggcc cttccgtttc tttgct
LOCUS dna 8026 bp FEATURES Location/Qualifiers Other Gene 659..1468 /gene="psi_plus_pack other" ORF 1449..2216 /sequence="ORF_3 rf(6)" Marker 1566..2165 /gene="puro marker" Promoter 2787..2868 /gene="CMV prom" misc_binding 2894..2899 /dbxref="REBASE:HindIII" Other Gene 2913..3037 /gene="attR1 other" Other Gene 2937..3037 /gene="attR2 other" Marker 3146..3805 /gene="CAT marker" ORF 3146..3805 /sequence="ORF_1 rf(2)" Other Gene 4147..4452 /gene="ccdB other" Other Gene 4493..4612 /gene="attR1 other" Other Gene 4493..4593 /gene="attR2 other" misc_binding 4625..4630 /dbxref="REBASE:XhoI" misc_binding 4720..4725 /dbxref="REBASE:ClaI" Other Gene 4807..5358 /gene="5_LTR2 other" misc_binding 5062..5067 /dbxref="REBASE:XbaI" Other Gene 5611..5644 /gene="loxP other" Other Gene 5704..5737 /gene="loxP other" Rep_Origin 6013..6632 /gene="pBR322 origin" Marker 6787..7647 /gene="amp marker" ORF 6787..7647 /sequence="ORF_2 rf(5)" Promoter 7689..7717 /gene="amp prom" BASE COUNT 1938 a 2167 c 2009 g 1912 t 0 others ORIGIN 1 tttgaaagac cccacccgta ggtggcaagc tagcttaagt aacgccactt tgcaaggcat 61 ggaaaaatac ataactgaga atagaaaagt tcagatcaag gtcaggaaca aagaaacagc 121 tgaataccaa acaggatatc tgtggtaagc ggttcctgcc ccggctcagg gccaagaaca 181 gatgagacag ctgagtgatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg 241 ctcggggcca agaacagatg gtccccagat gcggtccagc cctcagcagt ttctagtgaa 301 tcatcagatg tttccagggt gccccaagga cctgaaaatg accctgtacc ttatttgaac 361 taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc cgctctccga gctcaataaa 421 agagcccaca acccctcact cggcgcgcca gtcttccgat agactgcgtc gcccgggtac 481 ccgtattccc aataaagcct cttgctgttt gcatccgaat cgtggtctcg ctgttccttg 541 ggagggtctc ctctgagtga ttgactaccc acgacggggg tctttcattt gggggctcgt 601 ccgggatttg gagacccctg cccagggacc accgacccac caccgggagg taagctggcc 661 agcaacttat ctgtgtctgt ccgattgtct agtgtctatg tttgatgtta tgcgcctgcg 721 tctgtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa ctgacgagtt 781 ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc gtttttgtgg 841 cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg tggttctggt 901 aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt cggtttggaa 961 ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct 1021 gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc ccttaagttt 1081 gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa 1141 gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg tcggatggcc 1201 gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg tcttttcacc 1261 tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag ccttggcttt 1321 tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc ctcttcctcc 1381 atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta 1441 tccagccctc actccttctc taggcgccgg aattgatccc ccgggctgca ggtcggccgc 1501 cacgaccggt gccgccacca tcccctgacc cacgcccctg acccctcaca aggagacgac 1561 cttccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtcccccggg 1621 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgacc 1681 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc 1741 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc 1801 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga 1861 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca 1921 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc 1981 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct 2041 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca 2101 ccgccgacgt cgagtgcccg aaggaccgcg cgacctggtg catgacccgc aagcccggtg 2161 cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga ccccatggct 2221 ccgaccgaag ccgacccggg cggccccgcc gaccccgcac ccgcccccga ggcccaccga 2281 ctctagtaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc 2341 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg 2401 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa 2461 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca 2521 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac 2581 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc 2641 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga 2701 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg 2761 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta 2821 cggtgggagg tctatataag cagagctggt ttagtgaacc gtcagatccg ctagcgctac 2881 cggactcaga tctaagcttc ctagcgatat caacaagttt gtacaaaaaa gctgaacgag 2941 aaacgtaaaa tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta 3001 cataatactg taaaacacaa catatccagt cactatggcg gccgcattag gcaccccagg 3061 ctttacactt tatgcttccg gctcgtataa tgtgtggatt ttgagttagg atccgtcgag 3121 attttcagga gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga 3181 tatatcccaa tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac 3241 ctataaccag accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa 3301 gcacaagttt tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga 3361 attccgtatg gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta 3421 caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga 3481 tttccggcag tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc 3541 ctatttccct aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag 3601 tttcaccagt tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac 3661 catgggcaaa tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca 3721 tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg 3781 cgatgagtgg cagggcgggg cgtaaagatc tggatccggc ttactaaaag ccagataaca 3841 gtatgcgtat ttgcgcgctg atttttgcgg tataagaata tatactgata tgtatacccg 3901 aagtatgtca aaaagaggta tgctatgaag cagcgtatta cagtgacagt tgacagcgac 3961 agctatcagt tgctcaaggc atatatgatg tcaatatctc cggtctggta agcacaacca 4021 tgcagaatga agcccgtcgt ctgcgtgccg aacgctggaa agcggaaaat caggaaggga 4081 tggctgaggt cgcccggttt attgaaatga acggctcttt tgctgacgag aacaggggct 4141 ggtgaaatgc agtttaaggt ttacacctat aaaagagaga gccgttatcg tctgtttgtg 4201 gatgtacaga gtgatattat tgacacgccc gggcgacgga tggtgatccc cctggccagt 4261 gcacgtctgc tgtcagataa agtctcccgt gaactttacc cggtggtgca tatcggggat 4321 gaaagctggc gcatgatgac caccgatatg gccagtgtgc cggtctccgt tatcggggaa 4381 gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc 4441 tggggaatat aaatgtcagg ctcccttata cacagccagt ctgcaggtcg accatagtga 4501 ctggatatgt tgtgttttac agtattatgt agtctgtttt ttatgcaaaa tctaatttaa 4561 tatattgata tttatatcat tttacgtttc tcgttcagct ttcttgtaca aagtggttga 4621 tatcctcgag tacccatacg acgtcccaga ctacgcttga gtttaaacga attcgttagg 4681 ccattaaggc ctgtcgacaa gcggccgcct cggccaaaca tcgataaaat aaaagatttt 4741 atttagtctc cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg gcaagctagc 4801 ttaagtaacg ccattttgca aggcatggaa aaatacataa ctgagaatag agaagttcag 4861 atcaaggtca ggaacagatg gaacagctga atatgggcca aacaggatat ctgtggtaag 4921 cagttcctgc cccggctcag ggccaagaac agatggaaca gctgaatatg ggccaaacag 4981 gatatctgtg gtaagcagtt cctgccccgg ctcagggcca agaacagatg gtccccagat 5041 gcggtccagc cctcagcagt ttctagagaa ccatcagatg tttccagggt gccccaagga 5101 cctgaaatga ccctgtgcct tatttgaact aaccaatcag ttcgcttctc gcttctgttc 5161 gcgcgcttct gctccccgag ctcaataaaa gagcccacaa cccctcactc ggggcgccag 5221 tcctccgatt gactgagtcg cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc 5281 atccgacttg tggtctcgct gttccttggg agggtctcct ctgagtgatt gactacccgt 5341 cagcgggggt ctttcatttg ggggctcgtc cgggatcggg agacccctgc ccagggacca 5401 ccgacccacc accgggaggt aagctggctg cctcgcgcgt ttcggtgatg acggtgaaaa 5461 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag 5521 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac 5581 ccagtcacgt agcgatagcg gagtgtactg ataacttcgt ataatgtatg ctatacgaag 5641 ttattaggtc tgaagaggag tttacgtcca gccaagctag ggccgcgatc cggaaccctt 5701 aatataactt cgtataatgt atgctatacg aagttatcag tactggctta actatgcggc 5761 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt 5821 aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc 5881 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 5941 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 6001 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca 6061 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 6121 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 6181 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta 6241 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 6301 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 6361 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 6421 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg 6481 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 6541 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag 6601 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa 6661 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat 6721 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc 6781 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc 6841 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc 6901 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc 6961 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc 7021 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt 7081 gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc 7141 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa 7201 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt 7261 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg 7321 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc 7381 gagttgctct tgcccggcgt caacacggga taataccgcg ccacatagca gaactttaaa 7441 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt 7501 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt 7561 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag 7621 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta 7681 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat 7741 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat 7801 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtcttc aagaattgct 7861 agcaattgct agcaattgct agcaattcat accagatcac cgaaaactgt cctccaaatg 7921 tgtccccctc acactcccaa attcgcgggc ttctgcctct tagaccactc taccctattc 7981 cccacactca ccggagccaa agccgcggcc cttccgtttc tttgct //
***Note: The text map shown below is representative of the parental
vector only and does not include the gene of interest. The sequence of the gene
can be found through the accession number link on the product page.
1 tttgaaagaccccacccgtaggtggcaagctagcttaagtaacgccactttgcaaggcat 60 AAACTTTCTGGGGTGGGCATCCACCGTTCGATCGAATTCATTGCGGTGAAACGTTCCGTA 61 ggaaaaatacataactgagaatagaaaagttcagatcaaggtcaggaacaaagaaacagc 120 CCTTTTTATGTATTGACTCTTATCTTTTCAAGTCTAGTTCCAGTCCTTGTTTCTTTGTCG 121 tgaataccaaacaggatatctgtggtaagcggttcctgccccggctcagggccaagaaca 180 ACTTATGGTTTGTCCTATAGACACCATTCGCCAAGGACGGGGCCGAGTCCCGGTTCTTGT 181 gatgagacagctgagtgatgggccaaacaggatatctgtggtaagcagttcctgccccgg 240 CTACTCTGTCGACTCACTACCCGGTTTGTCCTATAGACACCATTCGTCAAGGACGGGGCC 241 ctcggggccaagaacagatggtccccagatgcggtccagccctcagcagtttctagtgaa 300 GAGCCCCGGTTCTTGTCTACCAGGGGTCTACGCCAGGTCGGGAGTCGTCAAAGATCACTT 301 tcatcagatgtttccagggtgccccaaggacctgaaaatgaccctgtaccttatttgaac 360 AGTAGTCTACAAAGGTCCCACGGGGTTCCTGGACTTTTACTGGGACATGGAATAAACTTG 361 taaccaatcagttcgcttctcgcttctgttcgcgcgcttccgctctccgagctcaataaa 420 ATTGGTTAGTCAAGCGAAGAGCGAAGACAAGCGCGCGAAGGCGAGAGGCTCGAGTTATTT 421 agagcccacaacccctcactcggcgcgccagtcttccgatagactgcgtcgcccgggtac 480 TCTCGGGTGTTGGGGAGTGAGCCGCGCGGTCAGAAGGCTATCTGACGCAGCGGGCCCATG 481 ccgtattcccaataaagcctcttgctgtttgcatccgaatcgtggtctcgctgttccttg 540 GGCATAAGGGTTATTTCGGAGAACGACAAACGTAGGCTTAGCACCAGAGCGACAAGGAAC 541 ggagggtctcctctgagtgattgactacccacgacgggggtctttcatttgggggctcgt 600 CCTCCCAGAGGAGACTCACTAACTGATGGGTGCTGCCCCCAGAAAGTAAACCCCCGAGCA psi_plus_pack other(659,1468)>>> | 601 ccgggatttggagacccctgcccagggaccaccgacccaccaccgggaggtaagctggcc 660 GGCCCTAAACCTCTGGGGACGGGTCCCTGGTGGCTGGGTGGTGGCCCTCCATTCGACCGG 661 agcaacttatctgtgtctgtccgattgtctagtgtctatgtttgatgttatgcgcctgcg 720 TCGTTGAATAGACACAGACAGGCTAACAGATCACAGATACAAACTACAATACGCGGACGC 721 tctgtactagttagctaactagctctgtatctggcggacccgtggtggaactgacgagtt 780 AGACATGATCAATCGATTGATCGAGACATAGACCGCCTGGGCACCACCTTGACTGCTCAA 781 ctgaacacccggccgcaaccctgggagacgtcccagggactttgggggccgtttttgtgg 840 GACTTGTGGGCCGGCGTTGGGACCCTCTGCAGGGTCCCTGAAACCCCCGGCAAAAACACC 841 cccgacctgaggaagggagtcgatgtggaatccgaccccgtcaggatatgtggttctggt 900 GGGCTGGACTCCTTCCCTCAGCTACACCTTAGGCTGGGGCAGTCCTATACACCAAGACCA 901 aggagacgagaacctaaaacagttcccgcctccgtctgaatttttgctttcggtttggaa 960 TCCTCTGCTCTTGGATTTTGTCAAGGGCGGAGGCAGACTTAAAAACGAAAGCCAAACCTT 961 ccgaagccgcgcgtcttgtctgctgcagcgctgcagcatcgttctgtgttgtctctgtct 1020 GGCTTCGGCGCGCAGAACAGACGACGTCGCGACGTCGTAGCAAGACACAACAGAGACAGA 1021 gactgtgtttctgtatttgtctgaaaattagggccagactgttaccactcccttaagttt 1080 CTGACACAAAGACATAAACAGACTTTTAATCCCGGTCTGACAATGGTGAGGGAATTCAAA 1081 gaccttaggtcactggaaagatgtcgagcggatcgctcacaaccagtcggtagatgtcaa 1140 CTGGAATCCAGTGACCTTTCTACAGCTCGCCTAGCGAGTGTTGGTCAGCCATCTACAGTT 1141 gaagagacgttgggttaccttctgctctgcagaatggccaacctttaacgtcggatggcc 1200 CTTCTCTGCAACCCAATGGAAGACGAGACGTCTTACCGGTTGGAAATTGCAGCCTACCGG 1201 gcgagacggcacctttaaccgagacctcatcacccaggttaagatcaaggtcttttcacc 1260 CGCTCTGCCGTGGAAATTGGCTCTGGAGTAGTGGGTCCAATTCTAGTTCCAGAAAAGTGG 1261 tggcccgcatggacacccagaccaggtcccctacatcgtgacctgggaagccttggcttt 1320 ACCGGGCGTACCTGTGGGTCTGGTCCAGGGGATGTAGCACTGGACCCTTCGGAACCGAAA 1321 tgacccccctccctgggtcaagccctttgtacaccctaagcctccgcctcctcttcctcc 1380 ACTGGGGGGAGGGACCCAGTTCGGGAAACATGTGGGATTCGGAGGCGGAGGAGAAGGAGG 1381 atccgccccgtctctcccccttgaacctcctcgttcgaccccgcctcgatcctcccttta 1440 TAGGCGGGGCAGAGAGGGGGAACTTGGAGGAGCAAGCTGGGGCGGAGCTAGGAGGGAAAT ORF_3 rf(6)(1449,2216)<<< | 1441 tccagccctcactccttctctaggcgccggaattgatcccccgggctgcaggtcggccgc 1500 AGGTCGGGAGTGAGGAAGAGATCCGCGGCCTTAACTAGGGGGCCCGACGTCCAGCCGGCG 1501 cacgaccggtgccgccaccatcccctgacccacgcccctgacccctcacaaggagacgac 1560 GTGCTGGCCACGGCGGTGGTAGGGGACTGGGTGCGGGGACTGGGGAGTGTTCCTCTGCTG puro marker(1566,2165)>>> | 1561 cttccatgaccgagtacaagcccacggtgcgcctcgccacccgcgacgacgtcccccggg 1620 GAAGGTACTGGCTCATGTTCGGGTGCCACGCGGAGCGGTGGGCGCTGCTGCAGGGGGCCC 1621 ccgtacgcaccctcgccgccgcgttcgccgactaccccgccacgcgccacaccgtcgacc 1680 GGCATGCGTGGGAGCGGCGGCGCAAGCGGCTGATGGGGCGGTGCGCGGTGTGGCAGCTGG 1681 cggaccgccacatcgagcgggtcaccgagctgcaagaactcttcctcacgcgcgtcgggc 1740 GCCTGGCGGTGTAGCTCGCCCAGTGGCTCGACGTTCTTGAGAAGGAGTGCGCGCAGCCCG 1741 tcgacatcggcaaggtgtgggtcgcggacgacggcgccgcggtggcggtctggaccacgc 1800 AGCTGTAGCCGTTCCACACCCAGCGCCTGCTGCCGCGGCGCCACCGCCAGACCTGGTGCG 1801 cggagagcgtcgaagcgggggcggtgttcgccgagatcggcccgcgcatggccgagttga 1860 GCCTCTCGCAGCTTCGCCCCCGCCACAAGCGGCTCTAGCCGGGCGCGTACCGGCTCAACT 1861 gcggttcccggctggccgcgcagcaacagatggaaggcctcctggcgccgcaccggccca 1920 CGCCAAGGGCCGACCGGCGCGTCGTTGTCTACCTTCCGGAGGACCGCGGCGTGGCCGGGT 1921 aggagcccgcgtggttcctggccaccgtcggcgtctcgcccgaccaccagggcaagggtc 1980 TCCTCGGGCGCACCAAGGACCGGTGGCAGCCGCAGAGCGGGCTGGTGGTCCCGTTCCCAG 1981 tgggcagcgccgtcgtgctccccggagtggaggcggccgagcgcgccggggtgcccgcct 2040 ACCCGTCGCGGCAGCACGAGGGGCCTCACCTCCGCCGGCTCGCGCGGCCCCACGGGCGGA 2041 tcctggagacctccgcgccccgcaacctccccttctacgagcggctcggcttcaccgtca 2100 AGGACCTCTGGAGGCGCGGGGCGTTGGAGGGGAAGATGCTCGCCGAGCCGAAGTGGCAGT 2101 ccgccgacgtcgagtgcccgaaggaccgcgcgacctggtgcatgacccgcaagcccggtg 2160 GGCGGCTGCAGCTCACGGGCTTCCTGGCGCGCTGGACCACGTACTGGGCGTTCGGGCCAC 2161 cctgacgcccgccccacgacccgcagcgcccgaccgaaaggagcgcacgaccccatggct 2220 GGACTGCGGGCGGGGTGCTGGGCGTCGCGGGCTGGCTTTCCTCGCGTGCTGGGGTACCGA 2221 ccgaccgaagccgacccgggcggccccgccgaccccgcacccgcccccgaggcccaccga 2280 GGCTGGCTTCGGCTGGGCCCGCCGGGGCGGCTGGGGCGTGGGCGGGGGCTCCGGGTGGCT 2281 ctctagtaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgc 2340 GAGATCATTATCATTAGTTAATGCCCCAGTAATCAAGTATCGGGTATATACCTCAAGGCG 2341 gttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattg 2400 CAATGTATTGAATGCCATTTACCGGGCGGACCGACTGGCGGGTTGCTGGGGGCGGGTAAC 2401 acgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaa 2460 TGCAGTTATTACTGCATACAAGGGTATCATTGCGGTTATCCCTGAAAGGTAACTGCAGTT 2461 tgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgcca 2520 ACCCACCTCATAAATGCCATTTGACGGGTGAACCGTCATGTAGTTCACATAGTATACGGT 2521 agtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtac 2580 TCATGCGGGGGATAACTGCAGTTACTGCCATTTACCGGGCGGACCGTAATACGGGTCATG 2581 atgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattacc 2640 TACTGGAATACCCTGAAAGGATGAACCGTCATGTAGATGCATAATCAGTAGCGATAATGG 2641 atggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacgggga 2700 TACCACTACGCCAAAACCGTCATGTAGTTACCCGCACCTATCGCCAAACTGAGTGCCCCT 2701 tttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgg 2760 AAAGGTTCAGAGGTGGGGTAACTGCAGTTACCCTCAAACAAAACCGTGGTTTTAGTTGCC CMV prom(2787,2868)>>> | 2761 gactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgta 2820 CTGAAAGGTTTTACAGCATTGTTGAGGCGGGGTAACTGCGTTTACCCGCCATCCGCACAT 2821 cggtgggaggtctatataagcagagctggtttagtgaaccgtcagatccgctagcgctac 2880 GCCACCCTCCAGATATATTCGTCTCGACCAAATCACTTGGCAGTCTAGGCGATCGCGATG attR2 other(2937,3037)<<< | HindIII attR1 other(2913,3037)>>> | | | 2881 cggactcaGATCTAAGCTTCctagcgatatcaacaagtttgtacaaaaaagctgaacgag 2940 GCCTGAGTCTAGATTCGAAGGATCGCTATAGTTGTTCAAACATGTTTTTTCGACTTGCTC 2941 aaacgtaaaatgatataaatatcaatatattaaattagattttgcataaaaaacagacta 3000 TTTGCATTTTACTATATTTATAGTTATATAATTTAATCTAAAACGTATTTTTTGTCTGAT 3001 cataatactgtaaaacacaacatatccagtcactatggcggccgcattaggcaccccagg 3060 GTATTATGACATTTTGTGTTGTATAGGTCAGTGATACCGCCGGCGTAATCCGTGGGGTCC 3061 ctttacactttatgcttccggctcgtataatgtgtggattttgagttaggatccgtcgag 3120 GAAATGTGAAATACGAAGGCCGAGCATATTACACACCTAAAACTCAATCCTAGGCAGCTC CAT marker(3146,3805)>>> | ORF_1 rf(2)(3146,3805)>>> | 3121 attttcaggagctaaggaagctaaaatggagaaaaaaatcactggatataccaccgttga 3180 TAAAAGTCCTCGATTCCTTCGATTTTACCTCTTTTTTTAGTGACCTATATGGTGGCAACT 3181 tatatcccaatggcatcgtaaagaacattttgaggcatttcagtcagttgctcaatgtac 3240 ATATAGGGTTACCGTAGCATTTCTTGTAAAACTCCGTAAAGTCAGTCAACGAGTTACATG 3241 ctataaccagaccgttcagctggatattacggcctttttaaagaccgtaaagaaaaataa 3300 GATATTGGTCTGGCAAGTCGACCTATAATGCCGGAAAAATTTCTGGCATTTCTTTTTATT 3301 gcacaagttttatccggcctttattcacattcttgcccgcctgatgaatgctcatccgga 3360 CGTGTTCAAAATAGGCCGGAAATAAGTGTAAGAACGGGCGGACTACTTACGAGTAGGCCT 3361 attccgtatggcaatgaaagacggtgagctggtgatatgggatagtgttcacccttgtta 3420 TAAGGCATACCGTTACTTTCTGCCACTCGACCACTATACCCTATCACAAGTGGGAACAAT 3421 caccgttttccatgagcaaactgaaacgttttcatcgctctggagtgaataccacgacga 3480 GTGGCAAAAGGTACTCGTTTGACTTTGCAAAAGTAGCGAGACCTCACTTATGGTGCTGCT 3481 tttccggcagtttctacacatatattcgcaagatgtggcgtgttacggtgaaaacctggc 3540 AAAGGCCGTCAAAGATGTGTATATAAGCGTTCTACACCGCACAATGCCACTTTTGGACCG 3541 ctatttccctaaagggtttattgagaatatgtttttcgtctcagccaatccctgggtgag 3600 GATAAAGGGATTTCCCAAATAACTCTTATACAAAAAGCAGAGTCGGTTAGGGACCCACTC 3601 tttcaccagttttgatttaaacgtggccaatatggacaacttcttcgcccccgttttcac 3660 AAAGTGGTCAAAACTAAATTTGCACCGGTTATACCTGTTGAAGAAGCGGGGGCAAAAGTG 3661 catgggcaaatattatacgcaaggcgacaaggtgctgatgccgctggcgattcaggttca 3720 GTACCCGTTTATAATATGCGTTCCGCTGTTCCACGACTACGGCGACCGCTAAGTCCAAGT 3721 tcatgccgtttgtgatggcttccatgtcggcagaatgcttaatgaattacaacagtactg 3780 AGTACGGCAAACACTACCGAAGGTACAGCCGTCTTACGAATTACTTAATGTTGTCATGAC 3781 cgatgagtggcagggcggggcgtaaagatctggatccggcttactaaaagccagataaca 3840 GCTACTCACCGTCCCGCCCCGCATTTCTAGACCTAGGCCGAATGATTTTCGGTCTATTGT 3841 gtatgcgtatttgcgcgctgatttttgcggtataagaatatatactgatatgtatacccg 3900 CATACGCATAAACGCGCGACTAAAAACGCCATATTCTTATATATGACTATACATATGGGC 3901 aagtatgtcaaaaagaggtatgctatgaagcagcgtattacagtgacagttgacagcgac 3960 TTCATACAGTTTTTCTCCATACGATACTTCGTCGCATAATGTCACTGTCAACTGTCGCTG 3961 agctatcagttgctcaaggcatatatgatgtcaatatctccggtctggtaagcacaacca 4020 TCGATAGTCAACGAGTTCCGTATATACTACAGTTATAGAGGCCAGACCATTCGTGTTGGT 4021 tgcagaatgaagcccgtcgtctgcgtgccgaacgctggaaagcggaaaatcaggaaggga 4080 ACGTCTTACTTCGGGCAGCAGACGCACGGCTTGCGACCTTTCGCCTTTTAGTCCTTCCCT 4081 tggctgaggtcgcccggtttattgaaatgaacggctcttttgctgacgagaacaggggct 4140 ACCGACTCCAGCGGGCCAAATAACTTTACTTGCCGAGAAAACGACTGCTCTTGTCCCCGA ccdB other(4147,4452)>>> | 4141 ggtgaaatgcagtttaaggtttacacctataaaagagagagccgttatcgtctgtttgtg 4200 CCACTTTACGTCAAATTCCAAATGTGGATATTTTCTCTCTCGGCAATAGCAGACAAACAC 4201 gatgtacagagtgatattattgacacgcccgggcgacggatggtgatccccctggccagt 4260 CTACATGTCTCACTATAATAACTGTGCGGGCCCGCTGCCTACCACTAGGGGGACCGGTCA 4261 gcacgtctgctgtcagataaagtctcccgtgaactttacccggtggtgcatatcggggat 4320 CGTGCAGACGACAGTCTATTTCAGAGGGCACTTGAAATGGGCCACCACGTATAGCCCCTA 4321 gaaagctggcgcatgatgaccaccgatatggccagtgtgccggtctccgttatcggggaa 4380 CTTTCGACCGCGTACTACTGGTGGCTATACCGGTCACACGGCCAGAGGCAATAGCCCCTT 4381 gaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaacctgatgttc 4440 CTTCACCGACTAGAGTCGGTGGCGCTTTTACTGTAGTTTTTGCGGTAATTGGACTACAAG attR1 other(4493,4612)<<< | attR2 other(4493,4593)>>> | 4441 tggggaatataaatgtcaggctcccttatacacagccagtctgcaggtcgaccatagtga 4500 ACCCCTTATATTTACAGTCCGAGGGAATATGTGTCGGTCAGACGTCCAGCTGGTATCACT 4501 ctggatatgttgtgttttacagtattatgtagtctgttttttatgcaaaatctaatttaa 4560 GACCTATACAACACAAAATGTCATAATACATCAGACAAAAAATACGTTTTAGATTAAATT 4561 tatattgatatttatatcattttacgtttctcgttcagctttcttgtacaaagtggttga 4620 ATATAACTATAAATATAGTAAAATGCAAAGAGCAAGTCGAAAGAACATGTTTCACCAACT XhoI | 4621 tatcctcgagtacccatacgacgtcccagactacgcttgaGTTTAAACGaattcgttagg 4680 ATAGGAGCTCATGGGTATGCTGCAGGGTCTGATGCGAACTCAAATTTGCTTAAGCAATCC ClaI | 4681 ccattaaggcctgtcgacaagcggccgcctcggccaaacatcgataaaataaaagatttt 4740 GGTAATTCCGGACAGCTGTTCGCCGGCGGAGCCGGTTTGTAGCTATTTTATTTTCTAAAA 4741 atttagtctccagaaaaaggggggaatgaaagaccccacctgtaggtttggcaagctagc 4800 TAAATCAGAGGTCTTTTTCCCCCCTTACTTTCTGGGGTGGACATCCAAACCGTTCGATCG 5_LTR2 other(4807,5358)>>> | 4801 ttaagtaacgccattttgcaaggcatggaaaaatacataactgagaatagagaagttcag 4860 AATTCATTGCGGTAAAACGTTCCGTACCTTTTTATGTATTGACTCTTATCTCTTCAAGTC 4861 atcaaggtcaggaacagatggaacagctgaatatgggccaaacaggatatctgtggtaag 4920 TAGTTCCAGTCCTTGTCTACCTTGTCGACTTATACCCGGTTTGTCCTATAGACACCATTC 4921 cagttcctgccccggctcagggccaagaacagatggaacagctgaatatgggccaaacag 4980 GTCAAGGACGGGGCCGAGTCCCGGTTCTTGTCTACCTTGTCGACTTATACCCGGTTTGTC 4981 gatatctgtggtaagcagttcctgccccggctcagggccaagaacagatggtccccagat 5040 CTATAGACACCATTCGTCAAGGACGGGGCCGAGTCCCGGTTCTTGTCTACCAGGGGTCTA XbaI | 5041 gcggtccagccctcagcagtttctagagaaccatcagatgtttccagggtgccccaagga 5100 CGCCAGGTCGGGAGTCGTCAAAGATCTCTTGGTAGTCTACAAAGGTCCCACGGGGTTCCT 5101 cctgaaatgaccctgtgccttatttgaactaaccaatcagttcgcttctcgcttctgttc 5160 GGACTTTACTGGGACACGGAATAAACTTGATTGGTTAGTCAAGCGAAGAGCGAAGACAAG 5161 gcgcgcttctgctccccgagctcaataaaagagcccacaacccctcactcggggcgccag 5220 CGCGCGAAGACGAGGGGCTCGAGTTATTTTCTCGGGTGTTGGGGAGTGAGCCCCGCGGTC 5221 tcctccgattgactgagtcgcccgggtacccgtgtatccaataaaccctcttgcagttgc 5280 AGGAGGCTAACTGACTCAGCGGGCCCATGGGCACATAGGTTATTTGGGAGAACGTCAACG 5281 atccgacttgtggtctcgctgttccttgggagggtctcctctgagtgattgactacccgt 5340 TAGGCTGAACACCAGAGCGACAAGGAACCCTCCCAGAGGAGACTCACTAACTGATGGGCA 5341 cagcgggggtctttcatttgggggctcgtccgggatcgggagacccctgcccagggacca 5400 GTCGCCCCCAGAAAGTAAACCCCCGAGCAGGCCCTAGCCCTCTGGGGACGGGTCCCTGGT 5401 ccgacccaccaccgggaggtaagctggctgcctcgcgcgtttcggtgatgacggtgaaaa 5460 GGCTGGGTGGTGGCCCTCCATTCGACCGACGGAGCGCGCAAAGCCACTACTGCCACTTTT 5461 cctctgacacatgcagctcccggagacggtcacagcttgtctgtaagcggatgccgggag 5520 GGAGACTGTGTACGTCGAGGGCCTCTGCCAGTGTCGAACAGACATTCGCCTACGGCCCTC 5521 cagacaagcccgtcagggcgcgtcagcgggtgttggcgggtgtcggggcgcagccatgac 5580 GTCTGTTCGGGCAGTCCCGCGCAGTCGCCCACAACCGCCCACAGCCCCGCGTCGGTACTG loxP other(5611,5644)<<< | 5581 ccagtcacgtagcgatagcggagtgtactgataacttcgtataatgtatgctatacgaag 5640 GGTCAGTGCATCGCTATCGCCTCACATGACTATTGAAGCATATTACATACGATATGCTTC 5641 ttattaggtctgaagaggagtttacgtccagccaagctagggccgcgatccggaaccctt 5700 AATAATCCAGACTTCTCCTCAAATGCAGGTCGGTTCGATCCCGGCGCTAGGCCTTGGGAA loxP other(5704,5737)<<< | 5701 aatataacttcgtataatgtatgctatacgaagttatcagtactggcttaactatgcggc 5760 TTATATTGAAGCATATTACATACGATATGCTTCAATAGTCATGACCGAATTGATACGCCG 5761 atcagagcagattgtactgagagtgcaccatatgcggtgtgaaataccgcacagatgcgt 5820 TAGTCTCGTCTAACATGACTCTCACGTGGTATACGCCACACTTTATGGCGTGTCTACGCA 5821 aaggagaaaataccgcatcaggcgctcttccgcttcctcgctcactgactcgctgcgctc 5880 TTCCTCTTTTATGGCGTAGTCCGCGAGAAGGCGAAGGAGCGAGTGACTGAGCGACGCGAG 5881 ggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccac 5940 CCAGCAAGCCGACGCCGCTCGCCATAGTCGAGTGAGTTTCCGCCATTATGCCAATAGGTG 5941 agaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaa 6000 TCTTAGTCCCCTATTGCGTCCTTTCTTGTACACTCGTTTTCCGGTCGTTTTCCGGTCCTT pBR322 origin(6013,6632)<<< | 6001 ccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatca 6060 GGCATTTTTCCGGCGCAACGACCGCAAAAAGGTATCCGAGGCGGGGGGACTGCTCGTAGT 6061 caaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggc 6120 GTTTTTAGCTGCGAGTTCAGTCTCCACCGCTTTGGGCTGTCCTGATATTTCTATGGTCCG 6121 gtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggata 6180 CAAAGGGGGACCTTCGAGGGAGCACGCGAGAGGACAAGGCTGGGACGGCGAATGGCCTAT 6181 cctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggta 6240 GGACAGGCGGAAAGAGGGAAGCCCTTCGCACCGCGAAAGAGTATCGAGTGCGACATCCAT 6241 tctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttca 6300 AGAGTCAAGCCACATCCAGCAAGCGAGGTTCGACCCGACACACGTGCTTGGGGGGCAAGT 6301 gcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacga 6360 CGGGCTGGCGACGCGGAATAGGCCATTGATAGCAGAACTCAGGTTGGGCCATTCTGTGCT 6361 cttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcgg 6420 GAATAGCGGTGACCGTCGTCGGTGACCATTGTCCTAATCGTCTCGCTCCATACATCCGCC 6421 tgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttgg 6480 ACGATGTCTCAAGAACTTCACCACCGGATTGATGCCGATGTGATCTTCCTGTCATAAACC 6481 tatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccgg 6540 ATAGACGCGAGACGACTTCGGTCAATGGAAGCCTTTTTCTCAACCATCGAGAACTAGGCC 6541 caaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcag 6600 GTTTGTTTGGTGGCGACCATCGCCACCAAAAAAACAAACGTTCGTCGTCTAATGCGCGTC 6601 aaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaa 6660 TTTTTTTCCTAGAGTTCTTCTAGGAAACTAGAAAAGATGCCCCAGACTGCGAGTCACCTT 6661 cgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagat 6720 GCTTTTGAGTGCAATTCCCTAAAACCAGTACTCTAATAGTTTTTCCTAGAAGTGGATCTA 6721 ccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtc 6780 GGAAAATTTAATTTTTACTTCAAAATTTAGTTAGATTTCATATATACTCATTTGAACCAG amp marker(6787,7647)<<< | ORF_2 rf(5)(6787,7647)<<< | 6781 tgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttc 6840 ACTGTCAATGGTTACGAATTAGTCACTCCGTGGATAGAGTCGCTAGACAGATAAAGCAAG 6841 atccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatc 6900 TAGGTATCAACGGACTGAGGGGCAGCACATCTATTGATGCTATGCCCTCCCGAATGGTAG 6901 tggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagc 6960 ACCGGGGTCACGACGTTACTATGGCGCTCTGGGTGCGAGTGGCCGAGGTCTAAATAGTCG 6961 aataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctc 7020 TTATTTGGTCGGTCGGCCTTCCCGGCTCGCGTCTTCACCAGGACGTTGAAATAGGCGGAG 7021 catccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagttt 7080 GTAGGTCAGATAATTAACAACGGCCCTTCGATCTCATTCATCAAGCGGTCAATTATCAAA 7081 gcgcaacgttgttgccattgctgcaggcatcgtggtgtcacgctcgtcgtttggtatggc 7140 CGCGTTGCAACAACGGTAACGACGTCCGTAGCACCACAGTGCGAGCAGCAAACCATACCG 7141 ttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaa 7200 AAGTAAGTCGAGGCCAAGGGTTGCTAGTTCCGCTCAATGTACTAGGGGGTACAACACGTT 7201 aaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgtt 7260 TTTTCGCCAATCGAGGAAGCCAGGAGGCTAGCAACAGTCTTCATTCAACCGGCGTCACAA 7261 atcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatg 7320 TAGTGAGTACCAATACCGTCGTGACGTATTAAGAGAATGACAGTACGGTAGGCATTCTAC 7321 cttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgacc 7380 GAAAAGACACTGACCACTCATGAGTTGGTTCAGTAAGACTCTTATCACATACGCCGCTGG 7381 gagttgctcttgcccggcgtcaacacgggataataccgcgccacatagcagaactttaaa 7440 CTCAACGAGAACGGGCCGCAGTTGTGCCCTATTATGGCGCGGTGTATCGTCTTGAAATTT 7441 agtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgtt 7500 TCACGAGTAGTAACCTTTTGCAAGAAGCCCCGCTTTTGAGAGTTCCTAGAATGGCGACAA 7501 gagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttacttt 7560 CTCTAGGTCAAGCTACATTGGGTGAGCACGTGGGTTGACTAGAAGTCGTAGAAAATGAAA 7561 caccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataag 7620 GTGGTCGCAAAGACCCACTCGTTTTTGTCCTTCCGTTTTACGGCGTTTTTTCCCTTATTC 7621 ggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcattta 7680 CCGCTGTGCCTTTACAACTTATGAGTATGAGAAGGAAAAAGTTATAATAACTTCGTAAAT amp prom(7689,7717)<<< | 7681 tcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaat 7740 AGTCCCAATAACAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTA 7741 aggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattat 7800 TCCCCAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTGCAGATTCTTTGGTAATAATA 7801 catgacattaacctataaaaataggcgtatcacgaggccctttcgtcttcaagaattgct 7860 GTACTGTAATTGGATATTTTTATCCGCATAGTGCTCCGGGAAAGCAGAAGTTCTTAACGA 7861 agcaattgctagcaattgctagcaattcataccagatcaccgaaaactgtcctccaaatg 7920 TCGTTAACGATCGTTAACGATCGTTAAGTATGGTCTAGTGGCTTTTGACAGGAGGTTTAC 7921 tgtccccctcacactcccaaattcgcgggcttctgcctcttagaccactctaccctattc 7980 ACAGGGGGAGTGTGAGGGTTTAAGCGCCCGAAGACGGAGAATCTGGTGAGATGGGATAAG 7981 cccacactcaccggagccaaagccgcggcccttccgtttctttgct 8026 GGGTGTGAGTGGCCTCGGTTTCGGCGCCGGGAAGGCAAAGAAACGA
LOCUS dna 8026 bp FEATURES Location/Qualifiers Other Gene 659..1468 /gene="psi_plus_pack other" ORF 1449..2216 /sequence="ORF_3 rf(6)" Marker 1566..2165 /gene="puro marker" Promoter 2787..2868 /gene="CMV prom" misc_binding 2894..2899 /dbxref="REBASE:HindIII" Other Gene 2913..3037 /gene="attR1 other" Other Gene 2937..3037 /gene="attR2 other" Marker 3146..3805 /gene="CAT marker" ORF 3146..3805 /sequence="ORF_1 rf(2)" Other Gene 4147..4452 /gene="ccdB other" Other Gene 4493..4612 /gene="attR1 other" Other Gene 4493..4593 /gene="attR2 other" misc_binding 4625..4630 /dbxref="REBASE:XhoI" misc_binding 4720..4725 /dbxref="REBASE:ClaI" Other Gene 4807..5358 /gene="5_LTR2 other" misc_binding 5062..5067 /dbxref="REBASE:XbaI" Other Gene 5611..5644 /gene="loxP other" Other Gene 5704..5737 /gene="loxP other" Rep_Origin 6013..6632 /gene="pBR322 origin" Marker 6787..7647 /gene="amp marker" ORF 6787..7647 /sequence="ORF_2 rf(5)" Promoter 7689..7717 /gene="amp prom" BASE COUNT 1938 a 2167 c 2009 g 1912 t 0 others