pRetroG-CMV
| Vector Name | pRetroG-CMV |
| VectorType | Retroviral Plasmid |
| Antibiotic Information | Bacterial: Ampicillin Mammalian: Puromycin |
| Sequencing Primers | CMV sequencing primer 5`---CGC AAA TGG GCG GTA GGC GTG---3` |
| Additional Information | The gene expression is driven by a CMV promoter, without any Tag or reporter. Original Vector: 7999bp Vector without Insert Cassette: 6294bp. |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 tttgaaagac cccacccgta ggtggcaagc tagcttaagt aacgccactt tgcaaggcat 61 ggaaaaatac ataactgaga atagaaaagt tcagatcaag gtcaggaaca aagaaacagc 121 tgaataccaa acaggatatc tgtggtaagc ggttcctgcc ccggctcagg gccaagaaca 181 gatgagacag ctgagtgatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg 241 ctcggggcca agaacagatg gtccccagat gcggtccagc cctcagcagt ttctagtgaa 301 tcatcagatg tttccagggt gccccaagga cctgaaaatg accctgtacc ttatttgaac 361 taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc cgctctccga gctcaataaa 421 agagcccaca acccctcact cggcgcgcca gtcttccgat agactgcgtc gcccgggtac 481 ccgtattccc aataaagcct cttgctgttt gcatccgaat cgtggtctcg ctgttccttg 541 ggagggtctc ctctgagtga ttgactaccc acgacggggg tctttcattt gggggctcgt 601 ccgggatttg gagacccctg cccagggacc accgacccac caccgggagg taagctggcc 661 agcaacttat ctgtgtctgt ccgattgtct agtgtctatg tttgatgtta tgcgcctgcg 721 tctgtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa ctgacgagtt 781 ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc gtttttgtgg 841 cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg tggttctggt 901 aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt cggtttggaa 961 ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct 1021 gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc ccttaagttt 1081 gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa 1141 gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg tcggatggcc 1201 gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg tcttttcacc 1261 tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag ccttggcttt 1321 tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc ctcttcctcc 1381 atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta 1441 tccagccctc actccttctc taggcgccgg aattgatccc ccgggctgca ggtcggccgc 1501 cacgaccggt gccgccacca tcccctgacc cacgcccctg acccctcaca aggagacgac 1561 cttccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtcccccggg 1621 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgacc 1681 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc 1741 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc 1801 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga 1861 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca 1921 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc 1981 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct 2041 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca 2101 ccgccgacgt cgagtgcccg aaggaccgcg cgacctggtg catgacccgc aagcccggtg 2161 cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga ccccatggct 2221 ccgaccgaag ccgacccggg cggccccgcc gaccccgcac ccgcccccga ggcccaccga 2281 ctctagtaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc 2341 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg 2401 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa 2461 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca 2521 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac 2581 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc 2641 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga 2701 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg 2761 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta 2821 cggtgggagg tctatataag cagagctggt ttagtgaacc gtcagatccg ctagcgctac 2881 cggactcaga tctaagcttc ctagcgatat caacaagttt gtacaaaaaa gctgaacgag 2941 aaacgtaaaa tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta 3001 cataatactg taaaacacaa catatccagt cactatggcg gccgcattag gcaccccagg 3061 ctttacactt tatgcttccg gctcgtataa tgtgtggatt ttgagttagg atccgtcgag 3121 attttcagga gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga 3181 tatatcccaa tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac 3241 ctataaccag accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa 3301 gcacaagttt tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga 3361 attccgtatg gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta 3421 caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga 3481 tttccggcag tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc 3541 ctatttccct aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag 3601 tttcaccagt tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac 3661 catgggcaaa tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca 3721 tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg 3781 cgatgagtgg cagggcgggg cgtaaagatc tggatccggc ttactaaaag ccagataaca 3841 gtatgcgtat ttgcgcgctg atttttgcgg tataagaata tatactgata tgtatacccg 3901 aagtatgtca aaaagaggta tgctatgaag cagcgtatta cagtgacagt tgacagcgac 3961 agctatcagt tgctcaaggc atatatgatg tcaatatctc cggtctggta agcacaacca 4021 tgcagaatga agcccgtcgt ctgcgtgccg aacgctggaa agcggaaaat caggaaggga 4081 tggctgaggt cgcccggttt attgaaatga acggctcttt tgctgacgag aacaggggct 4141 ggtgaaatgc agtttaaggt ttacacctat aaaagagaga gccgttatcg tctgtttgtg 4201 gatgtacaga gtgatattat tgacacgccc gggcgacgga tggtgatccc cctggccagt 4261 gcacgtctgc tgtcagataa agtctcccgt gaactttacc cggtggtgca tatcggggat 4321 gaaagctggc gcatgatgac caccgatatg gccagtgtgc cggtctccgt tatcggggaa 4381 gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc 4441 tggggaatat aaatgtcagg ctcccttata cacagccagt ctgcaggtcg accatagtga 4501 ctggatatgt tgtgttttac agtattatgt agtctgtttt ttatgcaaaa tctaatttaa 4561 tatattgata tttatatcat tttacgtttc tcgttcagct ttcttgtaca aagtggttga 4621 tatctgactc gaggtttaaa cgaattcgtt aggccattaa ggcctgtcga caagcggccg 4681 cctcggccaa acatcgataa aataaaagat tttatttagt ctccagaaaa aggggggaat 4741 gaaagacccc acctgtaggt ttggcaagct agcttaagta acgccatttt gcaaggcatg 4801 gaaaaataca taactgagaa tagagaagtt cagatcaagg tcaggaacag atggaacagc 4861 tgaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct cagggccaag 4921 aacagatgga acagctgaat atgggccaaa caggatatct gtggtaagca gttcctgccc 4981 cggctcaggg ccaagaacag atggtcccca gatgcggtcc agccctcagc agtttctaga 5041 gaaccatcag atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg ccttatttga 5101 actaaccaat cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc gagctcaata 5161 aaagagccca caacccctca ctcggggcgc cagtcctccg attgactgag tcgcccgggt 5221 acccgtgtat ccaataaacc ctcttgcagt tgcatccgac ttgtggtctc gctgttcctt 5281 gggagggtct cctctgagtg attgactacc cgtcagcggg ggtctttcat ttgggggctc 5341 gtccgggatc gggagacccc tgcccaggga ccaccgaccc accaccggga ggtaagctgg 5401 ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac 5461 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc 5521 gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta 5581 ctgataactt cgtataatgt atgctatacg aagttattag gtctgaagag gagtttacgt 5641 ccagccaagc tagggccgcg atccggaacc cttaatataa cttcgtataa tgtatgctat 5701 acgaagttat cagtactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca 5761 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc 5821 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc 5881 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa 5941 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt 6001 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg 6061 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 6121 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 6181 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 6241 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 6301 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg 6361 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc 6421 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac 6481 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg 6541 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 6601 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 6661 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 6721 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga 6781 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt 6841 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg 6901 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga 6961 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga 7021 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctgcagg 7081 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc 7141 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc 7201 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca 7261 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac 7321 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg 7381 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc 7441 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg 7501 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac 7561 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat 7621 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata 7681 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa 7741 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg 7801 tatcacgagg ccctttcgtc ttcaagaatt gctagcaatt gctagcaatt gctagcaatt 7861 cataccagat caccgaaaac tgtcctccaa atgtgtcccc ctcacactcc caaattcgcg 7921 ggcttctgcc tcttagacca ctctacccta ttccccacac tcaccggagc caaagccgcg 7981 gcccttccgt ttctttgct
LOCUS dna 7999 bp FEATURES Location/Qualifiers Other Gene 659..1468 /gene="psi_plus_pack other" ORF 1449..2216 /sequence="ORF_3 rf(6)" Marker 1566..2165 /gene="puro marker" Promoter 2787..2868 /gene="CMV prom" misc_binding 2894..2899 /dbxref="REBASE:HindIII" Other Gene 2913..3037 /gene="attR1 other" Other Gene 2937..3037 /gene="attR2 other" Marker 3146..3805 /gene="CAT marker" ORF 3146..3805 /sequence="ORF_1 rf(2)" Other Gene 4147..4452 /gene="ccdB other" Other Gene 4493..4612 /gene="attR1 other" Other Gene 4493..4593 /gene="attR2 other" misc_binding 4628..4633 /dbxref="REBASE:XhoI" misc_binding 4693..4698 /dbxref="REBASE:ClaI" Other Gene 4780..5331 /gene="5_LTR2 other" misc_binding 5035..5040 /dbxref="REBASE:XbaI" Other Gene 5584..5617 /gene="loxP other" Other Gene 5677..5710 /gene="loxP other" Rep_Origin 5986..6605 /gene="pBR322 origin" Marker 6760..7620 /gene="amp marker" ORF 6760..7620 /sequence="ORF_2 rf(5)" Promoter 7662..7690 /gene="amp prom" BASE COUNT 1931 a 2156 c 2005 g 1907 t 0 others ORIGIN 1 tttgaaagac cccacccgta ggtggcaagc tagcttaagt aacgccactt tgcaaggcat 61 ggaaaaatac ataactgaga atagaaaagt tcagatcaag gtcaggaaca aagaaacagc 121 tgaataccaa acaggatatc tgtggtaagc ggttcctgcc ccggctcagg gccaagaaca 181 gatgagacag ctgagtgatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg 241 ctcggggcca agaacagatg gtccccagat gcggtccagc cctcagcagt ttctagtgaa 301 tcatcagatg tttccagggt gccccaagga cctgaaaatg accctgtacc ttatttgaac 361 taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc cgctctccga gctcaataaa 421 agagcccaca acccctcact cggcgcgcca gtcttccgat agactgcgtc gcccgggtac 481 ccgtattccc aataaagcct cttgctgttt gcatccgaat cgtggtctcg ctgttccttg 541 ggagggtctc ctctgagtga ttgactaccc acgacggggg tctttcattt gggggctcgt 601 ccgggatttg gagacccctg cccagggacc accgacccac caccgggagg taagctggcc 661 agcaacttat ctgtgtctgt ccgattgtct agtgtctatg tttgatgtta tgcgcctgcg 721 tctgtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa ctgacgagtt 781 ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc gtttttgtgg 841 cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg tggttctggt 901 aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt cggtttggaa 961 ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct 1021 gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc ccttaagttt 1081 gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa 1141 gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg tcggatggcc 1201 gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg tcttttcacc 1261 tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag ccttggcttt 1321 tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc ctcttcctcc 1381 atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta 1441 tccagccctc actccttctc taggcgccgg aattgatccc ccgggctgca ggtcggccgc 1501 cacgaccggt gccgccacca tcccctgacc cacgcccctg acccctcaca aggagacgac 1561 cttccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtcccccggg 1621 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgacc 1681 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc 1741 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc 1801 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga 1861 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca 1921 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc 1981 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct 2041 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca 2101 ccgccgacgt cgagtgcccg aaggaccgcg cgacctggtg catgacccgc aagcccggtg 2161 cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga ccccatggct 2221 ccgaccgaag ccgacccggg cggccccgcc gaccccgcac ccgcccccga ggcccaccga 2281 ctctagtaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc 2341 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg 2401 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa 2461 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca 2521 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac 2581 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc 2641 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga 2701 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg 2761 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta 2821 cggtgggagg tctatataag cagagctggt ttagtgaacc gtcagatccg ctagcgctac 2881 cggactcaga tctaagcttc ctagcgatat caacaagttt gtacaaaaaa gctgaacgag 2941 aaacgtaaaa tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta 3001 cataatactg taaaacacaa catatccagt cactatggcg gccgcattag gcaccccagg 3061 ctttacactt tatgcttccg gctcgtataa tgtgtggatt ttgagttagg atccgtcgag 3121 attttcagga gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga 3181 tatatcccaa tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac 3241 ctataaccag accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa 3301 gcacaagttt tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga 3361 attccgtatg gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta 3421 caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga 3481 tttccggcag tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc 3541 ctatttccct aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag 3601 tttcaccagt tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac 3661 catgggcaaa tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca 3721 tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg 3781 cgatgagtgg cagggcgggg cgtaaagatc tggatccggc ttactaaaag ccagataaca 3841 gtatgcgtat ttgcgcgctg atttttgcgg tataagaata tatactgata tgtatacccg 3901 aagtatgtca aaaagaggta tgctatgaag cagcgtatta cagtgacagt tgacagcgac 3961 agctatcagt tgctcaaggc atatatgatg tcaatatctc cggtctggta agcacaacca 4021 tgcagaatga agcccgtcgt ctgcgtgccg aacgctggaa agcggaaaat caggaaggga 4081 tggctgaggt cgcccggttt attgaaatga acggctcttt tgctgacgag aacaggggct 4141 ggtgaaatgc agtttaaggt ttacacctat aaaagagaga gccgttatcg tctgtttgtg 4201 gatgtacaga gtgatattat tgacacgccc gggcgacgga tggtgatccc cctggccagt 4261 gcacgtctgc tgtcagataa agtctcccgt gaactttacc cggtggtgca tatcggggat 4321 gaaagctggc gcatgatgac caccgatatg gccagtgtgc cggtctccgt tatcggggaa 4381 gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc 4441 tggggaatat aaatgtcagg ctcccttata cacagccagt ctgcaggtcg accatagtga 4501 ctggatatgt tgtgttttac agtattatgt agtctgtttt ttatgcaaaa tctaatttaa 4561 tatattgata tttatatcat tttacgtttc tcgttcagct ttcttgtaca aagtggttga 4621 tatctgactc gaggtttaaa cgaattcgtt aggccattaa ggcctgtcga caagcggccg 4681 cctcggccaa acatcgataa aataaaagat tttatttagt ctccagaaaa aggggggaat 4741 gaaagacccc acctgtaggt ttggcaagct agcttaagta acgccatttt gcaaggcatg 4801 gaaaaataca taactgagaa tagagaagtt cagatcaagg tcaggaacag atggaacagc 4861 tgaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct cagggccaag 4921 aacagatgga acagctgaat atgggccaaa caggatatct gtggtaagca gttcctgccc 4981 cggctcaggg ccaagaacag atggtcccca gatgcggtcc agccctcagc agtttctaga 5041 gaaccatcag atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg ccttatttga 5101 actaaccaat cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc gagctcaata 5161 aaagagccca caacccctca ctcggggcgc cagtcctccg attgactgag tcgcccgggt 5221 acccgtgtat ccaataaacc ctcttgcagt tgcatccgac ttgtggtctc gctgttcctt 5281 gggagggtct cctctgagtg attgactacc cgtcagcggg ggtctttcat ttgggggctc 5341 gtccgggatc gggagacccc tgcccaggga ccaccgaccc accaccggga ggtaagctgg 5401 ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac 5461 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc 5521 gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta 5581 ctgataactt cgtataatgt atgctatacg aagttattag gtctgaagag gagtttacgt 5641 ccagccaagc tagggccgcg atccggaacc cttaatataa cttcgtataa tgtatgctat 5701 acgaagttat cagtactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca 5761 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc 5821 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc 5881 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa 5941 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt 6001 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg 6061 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 6121 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 6181 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 6241 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 6301 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg 6361 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc 6421 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac 6481 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg 6541 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 6601 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 6661 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 6721 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga 6781 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt 6841 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg 6901 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga 6961 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga 7021 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctgcagg 7081 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc 7141 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc 7201 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca 7261 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac 7321 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg 7381 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc 7441 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg 7501 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac 7561 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat 7621 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata 7681 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa 7741 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg 7801 tatcacgagg ccctttcgtc ttcaagaatt gctagcaatt gctagcaatt gctagcaatt 7861 cataccagat caccgaaaac tgtcctccaa atgtgtcccc ctcacactcc caaattcgcg 7921 ggcttctgcc tcttagacca ctctacccta ttccccacac tcaccggagc caaagccgcg 7981 gcccttccgt ttctttgct //
***Note: The text map shown below is representative of the parental
vector only and does not include the gene of interest. The sequence of the gene
can be found through the accession number link on the product page.
1 tttgaaagaccccacccgtaggtggcaagctagcttaagtaacgccactttgcaaggcat 60 AAACTTTCTGGGGTGGGCATCCACCGTTCGATCGAATTCATTGCGGTGAAACGTTCCGTA 61 ggaaaaatacataactgagaatagaaaagttcagatcaaggtcaggaacaaagaaacagc 120 CCTTTTTATGTATTGACTCTTATCTTTTCAAGTCTAGTTCCAGTCCTTGTTTCTTTGTCG 121 tgaataccaaacaggatatctgtggtaagcggttcctgccccggctcagggccaagaaca 180 ACTTATGGTTTGTCCTATAGACACCATTCGCCAAGGACGGGGCCGAGTCCCGGTTCTTGT 181 gatgagacagctgagtgatgggccaaacaggatatctgtggtaagcagttcctgccccgg 240 CTACTCTGTCGACTCACTACCCGGTTTGTCCTATAGACACCATTCGTCAAGGACGGGGCC 241 ctcggggccaagaacagatggtccccagatgcggtccagccctcagcagtttctagtgaa 300 GAGCCCCGGTTCTTGTCTACCAGGGGTCTACGCCAGGTCGGGAGTCGTCAAAGATCACTT 301 tcatcagatgtttccagggtgccccaaggacctgaaaatgaccctgtaccttatttgaac 360 AGTAGTCTACAAAGGTCCCACGGGGTTCCTGGACTTTTACTGGGACATGGAATAAACTTG 361 taaccaatcagttcgcttctcgcttctgttcgcgcgcttccgctctccgagctcaataaa 420 ATTGGTTAGTCAAGCGAAGAGCGAAGACAAGCGCGCGAAGGCGAGAGGCTCGAGTTATTT 421 agagcccacaacccctcactcggcgcgccagtcttccgatagactgcgtcgcccgggtac 480 TCTCGGGTGTTGGGGAGTGAGCCGCGCGGTCAGAAGGCTATCTGACGCAGCGGGCCCATG 481 ccgtattcccaataaagcctcttgctgtttgcatccgaatcgtggtctcgctgttccttg 540 GGCATAAGGGTTATTTCGGAGAACGACAAACGTAGGCTTAGCACCAGAGCGACAAGGAAC 541 ggagggtctcctctgagtgattgactacccacgacgggggtctttcatttgggggctcgt 600 CCTCCCAGAGGAGACTCACTAACTGATGGGTGCTGCCCCCAGAAAGTAAACCCCCGAGCA psi_plus_pack other(659,1468)>>> | 601 ccgggatttggagacccctgcccagggaccaccgacccaccaccgggaggtaagctggcc 660 GGCCCTAAACCTCTGGGGACGGGTCCCTGGTGGCTGGGTGGTGGCCCTCCATTCGACCGG 661 agcaacttatctgtgtctgtccgattgtctagtgtctatgtttgatgttatgcgcctgcg 720 TCGTTGAATAGACACAGACAGGCTAACAGATCACAGATACAAACTACAATACGCGGACGC 721 tctgtactagttagctaactagctctgtatctggcggacccgtggtggaactgacgagtt 780 AGACATGATCAATCGATTGATCGAGACATAGACCGCCTGGGCACCACCTTGACTGCTCAA 781 ctgaacacccggccgcaaccctgggagacgtcccagggactttgggggccgtttttgtgg 840 GACTTGTGGGCCGGCGTTGGGACCCTCTGCAGGGTCCCTGAAACCCCCGGCAAAAACACC 841 cccgacctgaggaagggagtcgatgtggaatccgaccccgtcaggatatgtggttctggt 900 GGGCTGGACTCCTTCCCTCAGCTACACCTTAGGCTGGGGCAGTCCTATACACCAAGACCA 901 aggagacgagaacctaaaacagttcccgcctccgtctgaatttttgctttcggtttggaa 960 TCCTCTGCTCTTGGATTTTGTCAAGGGCGGAGGCAGACTTAAAAACGAAAGCCAAACCTT 961 ccgaagccgcgcgtcttgtctgctgcagcgctgcagcatcgttctgtgttgtctctgtct 1020 GGCTTCGGCGCGCAGAACAGACGACGTCGCGACGTCGTAGCAAGACACAACAGAGACAGA 1021 gactgtgtttctgtatttgtctgaaaattagggccagactgttaccactcccttaagttt 1080 CTGACACAAAGACATAAACAGACTTTTAATCCCGGTCTGACAATGGTGAGGGAATTCAAA 1081 gaccttaggtcactggaaagatgtcgagcggatcgctcacaaccagtcggtagatgtcaa 1140 CTGGAATCCAGTGACCTTTCTACAGCTCGCCTAGCGAGTGTTGGTCAGCCATCTACAGTT 1141 gaagagacgttgggttaccttctgctctgcagaatggccaacctttaacgtcggatggcc 1200 CTTCTCTGCAACCCAATGGAAGACGAGACGTCTTACCGGTTGGAAATTGCAGCCTACCGG 1201 gcgagacggcacctttaaccgagacctcatcacccaggttaagatcaaggtcttttcacc 1260 CGCTCTGCCGTGGAAATTGGCTCTGGAGTAGTGGGTCCAATTCTAGTTCCAGAAAAGTGG 1261 tggcccgcatggacacccagaccaggtcccctacatcgtgacctgggaagccttggcttt 1320 ACCGGGCGTACCTGTGGGTCTGGTCCAGGGGATGTAGCACTGGACCCTTCGGAACCGAAA 1321 tgacccccctccctgggtcaagccctttgtacaccctaagcctccgcctcctcttcctcc 1380 ACTGGGGGGAGGGACCCAGTTCGGGAAACATGTGGGATTCGGAGGCGGAGGAGAAGGAGG 1381 atccgccccgtctctcccccttgaacctcctcgttcgaccccgcctcgatcctcccttta 1440 TAGGCGGGGCAGAGAGGGGGAACTTGGAGGAGCAAGCTGGGGCGGAGCTAGGAGGGAAAT ORF_3 rf(6)(1449,2216)<<< | 1441 tccagccctcactccttctctaggcgccggaattgatcccccgggctgcaggtcggccgc 1500 AGGTCGGGAGTGAGGAAGAGATCCGCGGCCTTAACTAGGGGGCCCGACGTCCAGCCGGCG 1501 cacgaccggtgccgccaccatcccctgacccacgcccctgacccctcacaaggagacgac 1560 GTGCTGGCCACGGCGGTGGTAGGGGACTGGGTGCGGGGACTGGGGAGTGTTCCTCTGCTG puro marker(1566,2165)>>> | 1561 cttccatgaccgagtacaagcccacggtgcgcctcgccacccgcgacgacgtcccccggg 1620 GAAGGTACTGGCTCATGTTCGGGTGCCACGCGGAGCGGTGGGCGCTGCTGCAGGGGGCCC 1621 ccgtacgcaccctcgccgccgcgttcgccgactaccccgccacgcgccacaccgtcgacc 1680 GGCATGCGTGGGAGCGGCGGCGCAAGCGGCTGATGGGGCGGTGCGCGGTGTGGCAGCTGG 1681 cggaccgccacatcgagcgggtcaccgagctgcaagaactcttcctcacgcgcgtcgggc 1740 GCCTGGCGGTGTAGCTCGCCCAGTGGCTCGACGTTCTTGAGAAGGAGTGCGCGCAGCCCG 1741 tcgacatcggcaaggtgtgggtcgcggacgacggcgccgcggtggcggtctggaccacgc 1800 AGCTGTAGCCGTTCCACACCCAGCGCCTGCTGCCGCGGCGCCACCGCCAGACCTGGTGCG 1801 cggagagcgtcgaagcgggggcggtgttcgccgagatcggcccgcgcatggccgagttga 1860 GCCTCTCGCAGCTTCGCCCCCGCCACAAGCGGCTCTAGCCGGGCGCGTACCGGCTCAACT 1861 gcggttcccggctggccgcgcagcaacagatggaaggcctcctggcgccgcaccggccca 1920 CGCCAAGGGCCGACCGGCGCGTCGTTGTCTACCTTCCGGAGGACCGCGGCGTGGCCGGGT 1921 aggagcccgcgtggttcctggccaccgtcggcgtctcgcccgaccaccagggcaagggtc 1980 TCCTCGGGCGCACCAAGGACCGGTGGCAGCCGCAGAGCGGGCTGGTGGTCCCGTTCCCAG 1981 tgggcagcgccgtcgtgctccccggagtggaggcggccgagcgcgccggggtgcccgcct 2040 ACCCGTCGCGGCAGCACGAGGGGCCTCACCTCCGCCGGCTCGCGCGGCCCCACGGGCGGA 2041 tcctggagacctccgcgccccgcaacctccccttctacgagcggctcggcttcaccgtca 2100 AGGACCTCTGGAGGCGCGGGGCGTTGGAGGGGAAGATGCTCGCCGAGCCGAAGTGGCAGT 2101 ccgccgacgtcgagtgcccgaaggaccgcgcgacctggtgcatgacccgcaagcccggtg 2160 GGCGGCTGCAGCTCACGGGCTTCCTGGCGCGCTGGACCACGTACTGGGCGTTCGGGCCAC 2161 cctgacgcccgccccacgacccgcagcgcccgaccgaaaggagcgcacgaccccatggct 2220 GGACTGCGGGCGGGGTGCTGGGCGTCGCGGGCTGGCTTTCCTCGCGTGCTGGGGTACCGA 2221 ccgaccgaagccgacccgggcggccccgccgaccccgcacccgcccccgaggcccaccga 2280 GGCTGGCTTCGGCTGGGCCCGCCGGGGCGGCTGGGGCGTGGGCGGGGGCTCCGGGTGGCT 2281 ctctagtaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgc 2340 GAGATCATTATCATTAGTTAATGCCCCAGTAATCAAGTATCGGGTATATACCTCAAGGCG 2341 gttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattg 2400 CAATGTATTGAATGCCATTTACCGGGCGGACCGACTGGCGGGTTGCTGGGGGCGGGTAAC 2401 acgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaa 2460 TGCAGTTATTACTGCATACAAGGGTATCATTGCGGTTATCCCTGAAAGGTAACTGCAGTT 2461 tgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgcca 2520 ACCCACCTCATAAATGCCATTTGACGGGTGAACCGTCATGTAGTTCACATAGTATACGGT 2521 agtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtac 2580 TCATGCGGGGGATAACTGCAGTTACTGCCATTTACCGGGCGGACCGTAATACGGGTCATG 2581 atgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattacc 2640 TACTGGAATACCCTGAAAGGATGAACCGTCATGTAGATGCATAATCAGTAGCGATAATGG 2641 atggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacgggga 2700 TACCACTACGCCAAAACCGTCATGTAGTTACCCGCACCTATCGCCAAACTGAGTGCCCCT 2701 tttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgg 2760 AAAGGTTCAGAGGTGGGGTAACTGCAGTTACCCTCAAACAAAACCGTGGTTTTAGTTGCC CMV prom(2787,2868)>>> | 2761 gactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgta 2820 CTGAAAGGTTTTACAGCATTGTTGAGGCGGGGTAACTGCGTTTACCCGCCATCCGCACAT 2821 cggtgggaggtctatataagcagagctggtttagtgaaccgtcagatccgctagcgctac 2880 GCCACCCTCCAGATATATTCGTCTCGACCAAATCACTTGGCAGTCTAGGCGATCGCGATG attR2 other(2937,3037)<<< | HindIII attR1 other(2913,3037)>>> | | | 2881 cggactcaGATCTAAGCTTCctagcgatatcaacaagtttgtacaaaaaagctgaacgag 2940 GCCTGAGTCTAGATTCGAAGGATCGCTATAGTTGTTCAAACATGTTTTTTCGACTTGCTC 2941 aaacgtaaaatgatataaatatcaatatattaaattagattttgcataaaaaacagacta 3000 TTTGCATTTTACTATATTTATAGTTATATAATTTAATCTAAAACGTATTTTTTGTCTGAT 3001 cataatactgtaaaacacaacatatccagtcactatggcggccgcattaggcaccccagg 3060 GTATTATGACATTTTGTGTTGTATAGGTCAGTGATACCGCCGGCGTAATCCGTGGGGTCC 3061 ctttacactttatgcttccggctcgtataatgtgtggattttgagttaggatccgtcgag 3120 GAAATGTGAAATACGAAGGCCGAGCATATTACACACCTAAAACTCAATCCTAGGCAGCTC CAT marker(3146,3805)>>> | ORF_1 rf(2)(3146,3805)>>> | 3121 attttcaggagctaaggaagctaaaatggagaaaaaaatcactggatataccaccgttga 3180 TAAAAGTCCTCGATTCCTTCGATTTTACCTCTTTTTTTAGTGACCTATATGGTGGCAACT 3181 tatatcccaatggcatcgtaaagaacattttgaggcatttcagtcagttgctcaatgtac 3240 ATATAGGGTTACCGTAGCATTTCTTGTAAAACTCCGTAAAGTCAGTCAACGAGTTACATG 3241 ctataaccagaccgttcagctggatattacggcctttttaaagaccgtaaagaaaaataa 3300 GATATTGGTCTGGCAAGTCGACCTATAATGCCGGAAAAATTTCTGGCATTTCTTTTTATT 3301 gcacaagttttatccggcctttattcacattcttgcccgcctgatgaatgctcatccgga 3360 CGTGTTCAAAATAGGCCGGAAATAAGTGTAAGAACGGGCGGACTACTTACGAGTAGGCCT 3361 attccgtatggcaatgaaagacggtgagctggtgatatgggatagtgttcacccttgtta 3420 TAAGGCATACCGTTACTTTCTGCCACTCGACCACTATACCCTATCACAAGTGGGAACAAT 3421 caccgttttccatgagcaaactgaaacgttttcatcgctctggagtgaataccacgacga 3480 GTGGCAAAAGGTACTCGTTTGACTTTGCAAAAGTAGCGAGACCTCACTTATGGTGCTGCT 3481 tttccggcagtttctacacatatattcgcaagatgtggcgtgttacggtgaaaacctggc 3540 AAAGGCCGTCAAAGATGTGTATATAAGCGTTCTACACCGCACAATGCCACTTTTGGACCG 3541 ctatttccctaaagggtttattgagaatatgtttttcgtctcagccaatccctgggtgag 3600 GATAAAGGGATTTCCCAAATAACTCTTATACAAAAAGCAGAGTCGGTTAGGGACCCACTC 3601 tttcaccagttttgatttaaacgtggccaatatggacaacttcttcgcccccgttttcac 3660 AAAGTGGTCAAAACTAAATTTGCACCGGTTATACCTGTTGAAGAAGCGGGGGCAAAAGTG 3661 catgggcaaatattatacgcaaggcgacaaggtgctgatgccgctggcgattcaggttca 3720 GTACCCGTTTATAATATGCGTTCCGCTGTTCCACGACTACGGCGACCGCTAAGTCCAAGT 3721 tcatgccgtttgtgatggcttccatgtcggcagaatgcttaatgaattacaacagtactg 3780 AGTACGGCAAACACTACCGAAGGTACAGCCGTCTTACGAATTACTTAATGTTGTCATGAC 3781 cgatgagtggcagggcggggcgtaaagatctggatccggcttactaaaagccagataaca 3840 GCTACTCACCGTCCCGCCCCGCATTTCTAGACCTAGGCCGAATGATTTTCGGTCTATTGT 3841 gtatgcgtatttgcgcgctgatttttgcggtataagaatatatactgatatgtatacccg 3900 CATACGCATAAACGCGCGACTAAAAACGCCATATTCTTATATATGACTATACATATGGGC 3901 aagtatgtcaaaaagaggtatgctatgaagcagcgtattacagtgacagttgacagcgac 3960 TTCATACAGTTTTTCTCCATACGATACTTCGTCGCATAATGTCACTGTCAACTGTCGCTG 3961 agctatcagttgctcaaggcatatatgatgtcaatatctccggtctggtaagcacaacca 4020 TCGATAGTCAACGAGTTCCGTATATACTACAGTTATAGAGGCCAGACCATTCGTGTTGGT 4021 tgcagaatgaagcccgtcgtctgcgtgccgaacgctggaaagcggaaaatcaggaaggga 4080 ACGTCTTACTTCGGGCAGCAGACGCACGGCTTGCGACCTTTCGCCTTTTAGTCCTTCCCT 4081 tggctgaggtcgcccggtttattgaaatgaacggctcttttgctgacgagaacaggggct 4140 ACCGACTCCAGCGGGCCAAATAACTTTACTTGCCGAGAAAACGACTGCTCTTGTCCCCGA ccdB other(4147,4452)>>> | 4141 ggtgaaatgcagtttaaggtttacacctataaaagagagagccgttatcgtctgtttgtg 4200 CCACTTTACGTCAAATTCCAAATGTGGATATTTTCTCTCTCGGCAATAGCAGACAAACAC 4201 gatgtacagagtgatattattgacacgcccgggcgacggatggtgatccccctggccagt 4260 CTACATGTCTCACTATAATAACTGTGCGGGCCCGCTGCCTACCACTAGGGGGACCGGTCA 4261 gcacgtctgctgtcagataaagtctcccgtgaactttacccggtggtgcatatcggggat 4320 CGTGCAGACGACAGTCTATTTCAGAGGGCACTTGAAATGGGCCACCACGTATAGCCCCTA 4321 gaaagctggcgcatgatgaccaccgatatggccagtgtgccggtctccgttatcggggaa 4380 CTTTCGACCGCGTACTACTGGTGGCTATACCGGTCACACGGCCAGAGGCAATAGCCCCTT 4381 gaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaacctgatgttc 4440 CTTCACCGACTAGAGTCGGTGGCGCTTTTACTGTAGTTTTTGCGGTAATTGGACTACAAG attR1 other(4493,4612)<<< | attR2 other(4493,4593)>>> | 4441 tggggaatataaatgtcaggctcccttatacacagccagtctgcaggtcgaccatagtga 4500 ACCCCTTATATTTACAGTCCGAGGGAATATGTGTCGGTCAGACGTCCAGCTGGTATCACT 4501 ctggatatgttgtgttttacagtattatgtagtctgttttttatgcaaaatctaatttaa 4560 GACCTATACAACACAAAATGTCATAATACATCAGACAAAAAATACGTTTTAGATTAAATT 4561 tatattgatatttatatcattttacgtttctcgttcagctttcttgtacaaagtggttga 4620 ATATAACTATAAATATAGTAAAATGCAAAGAGCAAGTCGAAAGAACATGTTTCACCAACT XhoI | 4621 tatctgaCTCGAGGTTTAAACGaattcgttaggccattaaggcctgtcgacaagcggccg 4680 ATAGACTGAGCTCCAAATTTGCTTAAGCAATCCGGTAATTCCGGACAGCTGTTCGCCGGC ClaI | 4681 cctcggccaaacatcgataaaataaaagattttatttagtctccagaaaaaggggggaat 4740 GGAGCCGGTTTGTAGCTATTTTATTTTCTAAAATAAATCAGAGGTCTTTTTCCCCCCTTA 5_LTR2 other(4780,5331)>>> | 4741 gaaagaccccacctgtaggtttggcaagctagcttaagtaacgccattttgcaaggcatg 4800 CTTTCTGGGGTGGACATCCAAACCGTTCGATCGAATTCATTGCGGTAAAACGTTCCGTAC 4801 gaaaaatacataactgagaatagagaagttcagatcaaggtcaggaacagatggaacagc 4860 CTTTTTATGTATTGACTCTTATCTCTTCAAGTCTAGTTCCAGTCCTTGTCTACCTTGTCG 4861 tgaatatgggccaaacaggatatctgtggtaagcagttcctgccccggctcagggccaag 4920 ACTTATACCCGGTTTGTCCTATAGACACCATTCGTCAAGGACGGGGCCGAGTCCCGGTTC 4921 aacagatggaacagctgaatatgggccaaacaggatatctgtggtaagcagttcctgccc 4980 TTGTCTACCTTGTCGACTTATACCCGGTTTGTCCTATAGACACCATTCGTCAAGGACGGG XbaI | 4981 cggctcagggccaagaacagatggtccccagatgcggtccagccctcagcagtttctaga 5040 GCCGAGTCCCGGTTCTTGTCTACCAGGGGTCTACGCCAGGTCGGGAGTCGTCAAAGATCT 5041 gaaccatcagatgtttccagggtgccccaaggacctgaaatgaccctgtgccttatttga 5100 CTTGGTAGTCTACAAAGGTCCCACGGGGTTCCTGGACTTTACTGGGACACGGAATAAACT 5101 actaaccaatcagttcgcttctcgcttctgttcgcgcgcttctgctccccgagctcaata 5160 TGATTGGTTAGTCAAGCGAAGAGCGAAGACAAGCGCGCGAAGACGAGGGGCTCGAGTTAT 5161 aaagagcccacaacccctcactcggggcgccagtcctccgattgactgagtcgcccgggt 5220 TTTCTCGGGTGTTGGGGAGTGAGCCCCGCGGTCAGGAGGCTAACTGACTCAGCGGGCCCA 5221 acccgtgtatccaataaaccctcttgcagttgcatccgacttgtggtctcgctgttcctt 5280 TGGGCACATAGGTTATTTGGGAGAACGTCAACGTAGGCTGAACACCAGAGCGACAAGGAA 5281 gggagggtctcctctgagtgattgactacccgtcagcgggggtctttcatttgggggctc 5340 CCCTCCCAGAGGAGACTCACTAACTGATGGGCAGTCGCCCCCAGAAAGTAAACCCCCGAG 5341 gtccgggatcgggagacccctgcccagggaccaccgacccaccaccgggaggtaagctgg 5400 CAGGCCCTAGCCCTCTGGGGACGGGTCCCTGGTGGCTGGGTGGTGGCCCTCCATTCGACC 5401 ctgcctcgcgcgtttcggtgatgacggtgaaaacctctgacacatgcagctcccggagac 5460 GACGGAGCGCGCAAAGCCACTACTGCCACTTTTGGAGACTGTGTACGTCGAGGGCCTCTG 5461 ggtcacagcttgtctgtaagcggatgccgggagcagacaagcccgtcagggcgcgtcagc 5520 CCAGTGTCGAACAGACATTCGCCTACGGCCCTCGTCTGTTCGGGCAGTCCCGCGCAGTCG 5521 gggtgttggcgggtgtcggggcgcagccatgacccagtcacgtagcgatagcggagtgta 5580 CCCACAACCGCCCACAGCCCCGCGTCGGTACTGGGTCAGTGCATCGCTATCGCCTCACAT loxP other(5584,5617)<<< | 5581 ctgataacttcgtataatgtatgctatacgaagttattaggtctgaagaggagtttacgt 5640 GACTATTGAAGCATATTACATACGATATGCTTCAATAATCCAGACTTCTCCTCAAATGCA loxP other(5677,5710)<<< | 5641 ccagccaagctagggccgcgatccggaacccttaatataacttcgtataatgtatgctat 5700 GGTCGGTTCGATCCCGGCGCTAGGCCTTGGGAATTATATTGAAGCATATTACATACGATA 5701 acgaagttatcagtactggcttaactatgcggcatcagagcagattgtactgagagtgca 5760 TGCTTCAATAGTCATGACCGAATTGATACGCCGTAGTCTCGTCTAACATGACTCTCACGT 5761 ccatatgcggtgtgaaataccgcacagatgcgtaaggagaaaataccgcatcaggcgctc 5820 GGTATACGCCACACTTTATGGCGTGTCTACGCATTCCTCTTTTATGGCGTAGTCCGCGAG 5821 ttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatc 5880 AAGGCGAAGGAGCGAGTGACTGAGCGACGCGAGCCAGCAAGCCGACGCCGCTCGCCATAG 5881 agctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaa 5940 TCGAGTGAGTTTCCGCCATTATGCCAATAGGTGTCTTAGTCCCCTATTGCGTCCTTTCTT pBR322 origin(5986,6605)<<< | 5941 catgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtt 6000 GTACACTCGTTTTCCGGTCGTTTTCCGGTCCTTGGCATTTTTCCGGCGCAACGACCGCAA 6001 tttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtg 6060 AAAGGTATCCGAGGCGGGGGGACTGCTCGTAGTGTTTTTAGCTGCGAGTTCAGTCTCCAC 6061 gcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcg 6120 CGCTTTGGGCTGTCCTGATATTTCTATGGTCCGCAAAGGGGGACCTTCGAGGGAGCACGC 6121 ctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaag 6180 GAGAGGACAAGGCTGGGACGGCGAATGGCCTATGGACAGGCGGAAAGAGGGAAGCCCTTC 6181 cgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctc 6240 GCACCGCGAAAGAGTATCGAGTGCGACATCCATAGAGTCAAGCCACATCCAGCAAGCGAG 6241 caagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaa 6300 GTTCGACCCGACACACGTGCTTGGGGGGCAAGTCGGGCTGGCGACGCGGAATAGGCCATT 6301 ctatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactgg 6360 GATAGCAGAACTCAGGTTGGGCCATTCTGTGCTGAATAGCGGTGACCGTCGTCGGTGACC 6361 taacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcc 6420 ATTGTCCTAATCGTCTCGCTCCATACATCCGCCACGATGTCTCAAGAACTTCACCACCGG 6421 taactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttac 6480 ATTGATGCCGATGTGATCTTCCTGTCATAAACCATAGACGCGAGACGACTTCGGTCAATG 6481 cttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtgg 6540 GAAGCCTTTTTCTCAACCATCGAGAACTAGGCCGTTTGTTTGGTGGCGACCATCGCCACC 6541 tttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatccttt 6600 AAAAAAACAAACGTTCGTCGTCTAATGCGCGTCTTTTTTTCCTAGAGTTCTTCTAGGAAA 6601 gatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggt 6660 CTAGAAAAGATGCCCCAGACTGCGAGTCACCTTGCTTTTGAGTGCAATTCCCTAAAACCA 6661 catgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaa 6720 GTACTCTAATAGTTTTTCCTAGAAGTGGATCTAGGAAAATTTAATTTTTACTTCAAAATT amp marker(6760,7620)<<< | ORF_2 rf(5)(6760,7620)<<< | 6721 atcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtga 6780 TAGTTAGATTTCATATATACTCATTTGAACCAGACTGTCAATGGTTACGAATTAGTCACT 6781 ggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgt 6840 CCGTGGATAGAGTCGCTAGACAGATAAAGCAAGTAGGTATCAACGGACTGAGGGGCAGCA 6841 gtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcg 6900 CATCTATTGATGCTATGCCCTCCCGAATGGTAGACCGGGGTCACGACGTTACTATGGCGC 6901 agacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccga 6960 TCTGGGTGCGAGTGGCCGAGGTCTAAATAGTCGTTATTTGGTCGGTCGGCCTTCCCGGCT 6961 gcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccggga 7020 CGCGTCTTCACCAGGACGTTGAAATAGGCGGAGGTAGGTCAGATAATTAACAACGGCCCT 7021 agctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctgcagg 7080 TCGATCTCATTCATCAAGCGGTCAATTATCAAACGCGTTGCAACAACGGTAACGACGTCC 7081 catcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatc 7140 GTAGCACCACAGTGCGAGCAGCAAACCATACCGAAGTAAGTCGAGGCCAAGGGTTGCTAG 7141 aaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctcc 7200 TTCCGCTCAATGTACTAGGGGGTACAACACGTTTTTTCGCCAATCGAGGAAGCCAGGAGG 7201 gatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgca 7260 CTAGCAACAGTCTTCATTCAACCGGCGTCACAATAGTGAGTACCAATACCGTCGTGACGT 7261 taattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaac 7320 ATTAAGAGAATGACAGTACGGTAGGCATTCTACGAAAAGACACTGACCACTCATGAGTTG 7321 caagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaacacg 7380 GTTCAGTAAGACTCTTATCACATACGCCGCTGGCTCAACGAGAACGGGCCGCAGTTGTGC 7381 ggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttc 7440 CCTATTATGGCGCGGTGTATCGTCTTGAAATTTTCACGAGTAGTAACCTTTTGCAAGAAG 7441 ggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcg 7500 CCCCGCTTTTGAGAGTTCCTAGAATGGCGACAACTCTAGGTCAAGCTACATTGGGTGAGC 7501 tgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaac 7560 ACGTGGGTTGACTAGAAGTCGTAGAAAATGAAAGTGGTCGCAAAGACCCACTCGTTTTTG 7561 aggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcat 7620 TCCTTCCGTTTTACGGCGTTTTTTCCCTTATTCCCGCTGTGCCTTTACAACTTATGAGTA amp prom(7662,7690)<<< | 7621 actcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggata 7680 TGAGAAGGAAAAAGTTATAATAACTTCGTAAATAGTCCCAATAACAGAGTACTCGCCTAT 7681 catatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaa 7740 GTATAAACTTACATAAATCTTTTTATTTGTTTATCCCCAAGGCGCGTGTAAAGGGGCTTT 7741 agtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcg 7800 TCACGGTGGACTGCAGATTCTTTGGTAATAATAGTACTGTAATTGGATATTTTTATCCGC 7801 tatcacgaggccctttcgtcttcaagaattgctagcaattgctagcaattgctagcaatt 7860 ATAGTGCTCCGGGAAAGCAGAAGTTCTTAACGATCGTTAACGATCGTTAACGATCGTTAA 7861 cataccagatcaccgaaaactgtcctccaaatgtgtccccctcacactcccaaattcgcg 7920 GTATGGTCTAGTGGCTTTTGACAGGAGGTTTACACAGGGGGAGTGTGAGGGTTTAAGCGC 7921 ggcttctgcctcttagaccactctaccctattccccacactcaccggagccaaagccgcg 7980 CCGAAGACGGAGAATCTGGTGAGATGGGATAAGGGGTGTGAGTGGCCTCGGTTTCGGCGC 7981 gcccttccgtttctttgct 7999 CGGGAAGGCAAAGAAACGA
LOCUS dna 7999 bp FEATURES Location/Qualifiers Other Gene 659..1468 /gene="psi_plus_pack other" ORF 1449..2216 /sequence="ORF_3 rf(6)" Marker 1566..2165 /gene="puro marker" Promoter 2787..2868 /gene="CMV prom" misc_binding 2894..2899 /dbxref="REBASE:HindIII" Other Gene 2913..3037 /gene="attR1 other" Other Gene 2937..3037 /gene="attR2 other" Marker 3146..3805 /gene="CAT marker" ORF 3146..3805 /sequence="ORF_1 rf(2)" Other Gene 4147..4452 /gene="ccdB other" Other Gene 4493..4612 /gene="attR1 other" Other Gene 4493..4593 /gene="attR2 other" misc_binding 4628..4633 /dbxref="REBASE:XhoI" misc_binding 4693..4698 /dbxref="REBASE:ClaI" Other Gene 4780..5331 /gene="5_LTR2 other" misc_binding 5035..5040 /dbxref="REBASE:XbaI" Other Gene 5584..5617 /gene="loxP other" Other Gene 5677..5710 /gene="loxP other" Rep_Origin 5986..6605 /gene="pBR322 origin" Marker 6760..7620 /gene="amp marker" ORF 6760..7620 /sequence="ORF_2 rf(5)" Promoter 7662..7690 /gene="amp prom" BASE COUNT 1931 a 2156 c 2005 g 1907 t 0 others