pAAV-G-CMV-SV40-Luc-hGH-amp
| Vector Name | pAAV-G-CMV-SV40-Luc-hGH-amp |
| VectorType | AAV Plasmid |
| Antibiotic Information | Ampicillin |
| Sequencing Primers | CMV-F: CGCAAATGGGCGGTAGGCGTG |
| Additional Information | No Additional Information |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgccc taggcgttac ataacttacg gtaaatggcc cgcctggctg
181 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
241 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
301 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
361 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
421 ctacgtttag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
481 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
541 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
601 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg
661 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagaaccgag
721 tttaaactcc ctatcagtga tagagatctc cctatcagtg atagagagct agcgatatca
781 acaagtttgt acaaaaaagc tgaacgagaa acgtaaaatg atataaatat caatatatta
841 aattagattt tgcataaaaa acagactaca taatactgta aaacacaaca tatccagtca
901 ctatggcggc cgcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg
961 tgtggatttt gagttaggat ccgtcgagat tttcaggagc taaggaagct aaaatggaga
1021 aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg
1081 aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg
1141 cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc
1201 ttgcccgcct gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg
1261 tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt
1321 catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag
1381 atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt
1441 ttttcgtctc agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata
1501 tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg
1561 tgctgatgcc gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca
1621 gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg taaagatctg
1681 gatccggctt actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta
1741 taagaatata tactgatatg tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca
1801 gcgtattaca gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc
1861 aatatctccg gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa
1921 cgctggaaag cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac
1981 ggctcttttg ctgacgagaa caggggctgg tgaaatgcag tttaaggttt acacctataa
2041 aagagagagc cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg
2101 gcgacggatg gtgatccccc tggccagtgc acgtctgctg tcagataaag tctcccgtga
2161 actttacccg gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc
2221 cagtgtgccg gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga
2281 catcaaaaac gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca
2341 cagccagtct gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag
2401 tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc
2461 gttcagcttt cttgtacaaa gtggttgata tctgactcga gtacgcttga gtttaaacac
2521 gcgtggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
2581 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
2641 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
2701 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
2761 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
2821 ggaggcccaa ttggccacca tggaagatgc caaaaacatt aagaagggcc cagcgccatt
2881 ctacccactc gaagacggga ccgccggcga gcagctgcac aaagccatga agcgctacgc
2941 cctggtgccc ggcaccatcg cctttaccga cgcacatatc gaggtggaca ttacctacgc
3001 cgagtacttc gagatgagcg ttcggctggc agaagctatg aagcgctatg ggctgaatac
3061 aaaccatcgg atcgtggtgt gcagcgagaa tagcttgcag ttcttcatgc ccgtgttggg
3121 tgccctgttc atcggtgtgg ctgtggcccc agctaacgac atctacaacg agcgcgagct
3181 gctgaacagc atgggcatca gccagcccac cgtcgtattc gtgagcaaga aagggctgca
3241 aaagatcctc aacgtgcaaa agaagctacc gatcatacaa aagatcatca tcatggatag
3301 caagaccgac taccagggct tccaaagcat gtacaccttc gtgacttccc atttgccacc
3361 cggcttcaac gagtacgact tcgtgcccga gagcttcgac cgggacaaaa ccatcgccct
3421 gatcatgaac agtagtggca gtaccggatt gcccaagggc gtagccctac cgcaccgcac
3481 cgcttgtgtc cgattcagtc atgcccgcga ccccatcttc ggcaaccaga tcatccccga
3541 caccgctatc ctcagcgtgg tgccatttca ccacggcttc ggcatgttca ccacgctggg
3601 ctacttgatc tgcggctttc gggtcgtgct catgtaccgc ttcgaggagg agctattctt
3661 gcgcagcttg caagactata agattcaatc tgccctgctg gtgcccacac tatttagctt
3721 cttcgctaag agcactctca tcgacaagta cgacctaagc aacttgcacg agatcgccag
3781 cggcggggcg ccgctcagca aggaggtagg tgaggccgtg gccaaacgct tccacctacc
3841 aggcatccgc cagggctacg gcctgacaga aacaaccagc gccattctga tcacccccga
3901 aggggacgac aagcctggcg cagtaggcaa ggtggtgccc ttcttcgagg ctaaggtggt
3961 ggacttggac accggtaaga cactgggtgt gaaccagcgc ggcgagctgt gcgtccgtgg
4021 ccccatgatc atgagcggct acgttaacaa ccccgaggct acaaacgctc tcatcgacaa
4081 ggacggctgg ctgcacagcg gcgacatcgc ctactgggac gaggacgagc acttcttcat
4141 cgtggaccgg ctgaagagcc tgatcaaata caagggctac caggtagccc cagccgaact
4201 ggagagcatc ctgctgcaac accccaacat cttcgacgcc ggggtcgccg gcctgcccga
4261 cgacgatgcc ggcgagctgc ccgccgcagt cgtcgtgctg gaacacggta aaaccatgac
4321 cgagaaggag atcgtggact atgtggccag ccaggttaca accgccaaga agctgcgcgg
4381 tggtgttgtg ttcgtggacg aggtgcctaa aggactgacc ggcaagttgg acgcccgcaa
4441 gatccgcgag attctcatta aggccaagaa gggcggcaag atcgccgtgt aacttaaggg
4501 gtggcatccc tgtgacccct ccccagtgcc tctcctggcc ctggaagttg ccactccagt
4561 gcccaccagc cttgtcctaa taaaattaag ttgcatcatt ttgtctgact aggtgtcctt
4621 ctataatatt atggggtgga ggggggtggt atggagcaag gggcaagttg ggaagacaac
4681 ctgtagggcc tgcggggtct attgggaacc aagctggagt gcagtggcac aatcttggct
4741 cactgcaatc tccgcctcct gggttcaagc gattctcctg cctcagcctc ccgagttgtt
4801 gggattccag gcatgcatga ccaggctcag ctaatttttg tttttttggt agagacgggg
4861 tttcaccata ttggccaggc tggtctccaa ctcctaatct caggtgatct acccaccttg
4921 gcctcccaaa ttgctgggat tacaggcgtg aaccactgct cccttccctg tccttcacgt
4981 gcggaccgag cggccgcagg aacccctagt gatggagttg gccactccct ctctgcgcgc
5041 tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc
5101 ggcctcagtg agcgagcgag cgcgcagctg cctgcagggg cgcctgatgc ggtattttct
5161 ccttacgcat ctgtgcggta tttcacaccg catacgtcaa agcaaccata gtacgcgccc
5221 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
5281 gccagcgcct tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
5341 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
5401 cggcacctcg accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc
5461 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
5521 ttccaaactg gaacaacact caactctatc tcgggctatt cttttgattt ataagggatt
5581 ttgccgattt cggtctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
5641 tttaacaaaa tattaacgtt tacaatttta tggtgcactc tcagtacaat ctgctctgat
5701 gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
5761 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
5821 cagaggtttt caccgtcatc accgaaacgc gcgagccggg agctgcatgt gtcagaggtt
5881 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata
5941 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
6001 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
6061 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
6121 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
6181 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
6241 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
6301 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
6361 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
6421 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
6481 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
6541 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
6601 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
6661 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
6721 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
6781 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
6841 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
6901 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
6961 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
7021 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
7081 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
7141 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
7201 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
7261 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
7321 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
7381 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
7441 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
7501 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
7561 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
7621 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
7681 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
7741 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgt
LOCUS Exported File 7787 bp ds-DNA circular SYN 09-DEC-2019
DEFINITION Double floxed Gi-coupled hM4D DREADD fused with mCherry under the
control of human synapsin promoter..
ACCESSION .
VERSION .
KEYWORDS pAAV-G-CMV-SV40-Luc-hGH-amp
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7787)
AUTHORS Krashes MJ, Koda S, Ye C, Rogan SC, Adams AC, Cusher DS,
Maratos-Flier E, Roth BL, Lowell BB
TITLE Rapid, reversible activation of AgRP neurons drives feeding behavior
in mice.
JOURNAL J Clin Invest. 2011 Apr;121(4):1424-8. doi: 10.1172/JCI46229.
PUBMED 21364278
REFERENCE 2 (bases 1 to 7787)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Dec-26-19 from SnapGene 1.1.3
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..7787
/organism="synthetic DNA construct"
/mol_type="other DNA"
repeat_region 1..130
/note="AAV2 ITR (alternate)"
/note="color: #ffcc99"
enhancer 145..447
/note="CMV enhancer"
/note="color: #ffffff"
promoter 448..651
/note="CMV promoter"
/note="color: #ffffff; direction: RIGHT"
protein_bind 728..746
/note="tet operator"
/note="color: #31849b"
protein_bind 749..767
/note="tet operator"
/note="color: #31849b"
protein_bind 781..905
/note="attR1"
/note="color: #31849b; direction: RIGHT"
promoter 930..960
/note="lac UV5 promoter"
/note="This forward directional feature has 3 segments:
1: 930 .. 935 / #ffffff / -35
2: 936 .. 953 / #ffffff
3: 954 .. 960 / #ffffff / -10"
CDS 1014..1673
/codon_start=1
/note="CmR"
/note="color: #ccffcc"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
CDS 2015..2320
/codon_start=1
/note="ccdB"
/note="color: #993366"
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
protein_bind complement(2361..2485)
/note="attR2"
/note="color: #31849b; direction: LEFT"
promoter 2525..2822
/note="SV40 promoter"
/note="color: #ffffff; direction: RIGHT"
rep_origin 2692..2827
/note="SV40 ori"
/note="color: #ffff00"
CDS 2840..4492
/codon_start=1
/note="luciferase"
/note="color: #ffcc00"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
polyA_signal 4499..4975
/note="hGH poly(A) signal"
/note="color: #a6acb3"
repeat_region 4998..5138
/note="AAV2 ITR"
/note="color: #ffe4c4"
repeat_region 4998..5127
/note="AAV2 ITR"
/note="color: #ffcc99"
rep_origin 5213..5668
/direction=RIGHT
/note="f1 ori"
/note="color: #ffff00"
promoter 6002..6106
/note="AmpR promoter"
/note="color: #ffffff; direction: RIGHT"
CDS 6107..6967
/codon_start=1
/note="AmpR"
/note="This feature has 2 segments:
1: 6107 .. 6175 / #ccffcc / signal sequence
2: 6176 .. 6967 / #ccffcc"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 7138..7726
/direction=RIGHT
/note="ori"
/note="color: #ffff00"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgccc taggcgttac ataacttacg gtaaatggcc cgcctggctg
181 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
241 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
301 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
361 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
421 ctacgtttag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
481 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
541 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
601 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg
661 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagaaccgag
721 tttaaactcc ctatcagtga tagagatctc cctatcagtg atagagagct agcgatatca
781 acaagtttgt acaaaaaagc tgaacgagaa acgtaaaatg atataaatat caatatatta
841 aattagattt tgcataaaaa acagactaca taatactgta aaacacaaca tatccagtca
901 ctatggcggc cgcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg
961 tgtggatttt gagttaggat ccgtcgagat tttcaggagc taaggaagct aaaatggaga
1021 aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg
1081 aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg
1141 cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc
1201 ttgcccgcct gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg
1261 tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt
1321 catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag
1381 atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt
1441 ttttcgtctc agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata
1501 tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg
1561 tgctgatgcc gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca
1621 gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg taaagatctg
1681 gatccggctt actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta
1741 taagaatata tactgatatg tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca
1801 gcgtattaca gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc
1861 aatatctccg gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa
1921 cgctggaaag cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac
1981 ggctcttttg ctgacgagaa caggggctgg tgaaatgcag tttaaggttt acacctataa
2041 aagagagagc cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg
2101 gcgacggatg gtgatccccc tggccagtgc acgtctgctg tcagataaag tctcccgtga
2161 actttacccg gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc
2221 cagtgtgccg gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga
2281 catcaaaaac gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca
2341 cagccagtct gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag
2401 tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc
2461 gttcagcttt cttgtacaaa gtggttgata tctgactcga gtacgcttga gtttaaacac
2521 gcgtggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
2581 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
2641 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
2701 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
2761 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
2821 ggaggcccaa ttggccacca tggaagatgc caaaaacatt aagaagggcc cagcgccatt
2881 ctacccactc gaagacggga ccgccggcga gcagctgcac aaagccatga agcgctacgc
2941 cctggtgccc ggcaccatcg cctttaccga cgcacatatc gaggtggaca ttacctacgc
3001 cgagtacttc gagatgagcg ttcggctggc agaagctatg aagcgctatg ggctgaatac
3061 aaaccatcgg atcgtggtgt gcagcgagaa tagcttgcag ttcttcatgc ccgtgttggg
3121 tgccctgttc atcggtgtgg ctgtggcccc agctaacgac atctacaacg agcgcgagct
3181 gctgaacagc atgggcatca gccagcccac cgtcgtattc gtgagcaaga aagggctgca
3241 aaagatcctc aacgtgcaaa agaagctacc gatcatacaa aagatcatca tcatggatag
3301 caagaccgac taccagggct tccaaagcat gtacaccttc gtgacttccc atttgccacc
3361 cggcttcaac gagtacgact tcgtgcccga gagcttcgac cgggacaaaa ccatcgccct
3421 gatcatgaac agtagtggca gtaccggatt gcccaagggc gtagccctac cgcaccgcac
3481 cgcttgtgtc cgattcagtc atgcccgcga ccccatcttc ggcaaccaga tcatccccga
3541 caccgctatc ctcagcgtgg tgccatttca ccacggcttc ggcatgttca ccacgctggg
3601 ctacttgatc tgcggctttc gggtcgtgct catgtaccgc ttcgaggagg agctattctt
3661 gcgcagcttg caagactata agattcaatc tgccctgctg gtgcccacac tatttagctt
3721 cttcgctaag agcactctca tcgacaagta cgacctaagc aacttgcacg agatcgccag
3781 cggcggggcg ccgctcagca aggaggtagg tgaggccgtg gccaaacgct tccacctacc
3841 aggcatccgc cagggctacg gcctgacaga aacaaccagc gccattctga tcacccccga
3901 aggggacgac aagcctggcg cagtaggcaa ggtggtgccc ttcttcgagg ctaaggtggt
3961 ggacttggac accggtaaga cactgggtgt gaaccagcgc ggcgagctgt gcgtccgtgg
4021 ccccatgatc atgagcggct acgttaacaa ccccgaggct acaaacgctc tcatcgacaa
4081 ggacggctgg ctgcacagcg gcgacatcgc ctactgggac gaggacgagc acttcttcat
4141 cgtggaccgg ctgaagagcc tgatcaaata caagggctac caggtagccc cagccgaact
4201 ggagagcatc ctgctgcaac accccaacat cttcgacgcc ggggtcgccg gcctgcccga
4261 cgacgatgcc ggcgagctgc ccgccgcagt cgtcgtgctg gaacacggta aaaccatgac
4321 cgagaaggag atcgtggact atgtggccag ccaggttaca accgccaaga agctgcgcgg
4381 tggtgttgtg ttcgtggacg aggtgcctaa aggactgacc ggcaagttgg acgcccgcaa
4441 gatccgcgag attctcatta aggccaagaa gggcggcaag atcgccgtgt aacttaaggg
4501 gtggcatccc tgtgacccct ccccagtgcc tctcctggcc ctggaagttg ccactccagt
4561 gcccaccagc cttgtcctaa taaaattaag ttgcatcatt ttgtctgact aggtgtcctt
4621 ctataatatt atggggtgga ggggggtggt atggagcaag gggcaagttg ggaagacaac
4681 ctgtagggcc tgcggggtct attgggaacc aagctggagt gcagtggcac aatcttggct
4741 cactgcaatc tccgcctcct gggttcaagc gattctcctg cctcagcctc ccgagttgtt
4801 gggattccag gcatgcatga ccaggctcag ctaatttttg tttttttggt agagacgggg
4861 tttcaccata ttggccaggc tggtctccaa ctcctaatct caggtgatct acccaccttg
4921 gcctcccaaa ttgctgggat tacaggcgtg aaccactgct cccttccctg tccttcacgt
4981 gcggaccgag cggccgcagg aacccctagt gatggagttg gccactccct ctctgcgcgc
5041 tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc
5101 ggcctcagtg agcgagcgag cgcgcagctg cctgcagggg cgcctgatgc ggtattttct
5161 ccttacgcat ctgtgcggta tttcacaccg catacgtcaa agcaaccata gtacgcgccc
5221 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
5281 gccagcgcct tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
5341 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
5401 cggcacctcg accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc
5461 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
5521 ttccaaactg gaacaacact caactctatc tcgggctatt cttttgattt ataagggatt
5581 ttgccgattt cggtctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
5641 tttaacaaaa tattaacgtt tacaatttta tggtgcactc tcagtacaat ctgctctgat
5701 gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
5761 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
5821 cagaggtttt caccgtcatc accgaaacgc gcgagccggg agctgcatgt gtcagaggtt
5881 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata
5941 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
6001 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
6061 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
6121 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
6181 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
6241 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
6301 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
6361 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
6421 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
6481 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
6541 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
6601 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
6661 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
6721 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
6781 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
6841 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
6901 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
6961 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
7021 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
7081 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
7141 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
7201 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
7261 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
7321 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
7381 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
7441 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
7501 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
7561 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
7621 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
7681 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
7741 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgt
//
LOCUS Exported File 7787 bp ds-DNA circular SYN 09-DEC-2019
DEFINITION Double floxed Gi-coupled hM4D DREADD fused with mCherry under the
control of human synapsin promoter..
ACCESSION .
VERSION .
KEYWORDS pAAV-G-CMV-SV40-Luc-hGH-amp
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7787)
AUTHORS Krashes MJ, Koda S, Ye C, Rogan SC, Adams AC, Cusher DS,
Maratos-Flier E, Roth BL, Lowell BB
TITLE Rapid, reversible activation of AgRP neurons drives feeding behavior
in mice.
JOURNAL J Clin Invest. 2011 Apr;121(4):1424-8. doi: 10.1172/JCI46229.
PUBMED 21364278
REFERENCE 2 (bases 1 to 7787)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Dec-26-19 from SnapGene 1.1.3
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..7787
/organism="synthetic DNA construct"
/mol_type="other DNA"
repeat_region 1..130
/note="AAV2 ITR (alternate)"
/note="color: #ffcc99"
enhancer 145..447
/note="CMV enhancer"
/note="color: #ffffff"
promoter 448..651
/note="CMV promoter"
/note="color: #ffffff; direction: RIGHT"
protein_bind 728..746
/note="tet operator"
/note="color: #31849b"
protein_bind 749..767
/note="tet operator"
/note="color: #31849b"
protein_bind 781..905
/note="attR1"
/note="color: #31849b; direction: RIGHT"
promoter 930..960
/note="lac UV5 promoter"
/note="This forward directional feature has 3 segments:
1: 930 .. 935 / #ffffff / -35
2: 936 .. 953 / #ffffff
3: 954 .. 960 / #ffffff / -10"
CDS 1014..1673
/codon_start=1
/note="CmR"
/note="color: #ccffcc"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
CDS 2015..2320
/codon_start=1
/note="ccdB"
/note="color: #993366"
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
protein_bind complement(2361..2485)
/note="attR2"
/note="color: #31849b; direction: LEFT"
promoter 2525..2822
/note="SV40 promoter"
/note="color: #ffffff; direction: RIGHT"
rep_origin 2692..2827
/note="SV40 ori"
/note="color: #ffff00"
CDS 2840..4492
/codon_start=1
/note="luciferase"
/note="color: #ffcc00"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
polyA_signal 4499..4975
/note="hGH poly(A) signal"
/note="color: #a6acb3"
repeat_region 4998..5138
/note="AAV2 ITR"
/note="color: #ffe4c4"
repeat_region 4998..5127
/note="AAV2 ITR"
/note="color: #ffcc99"
rep_origin 5213..5668
/direction=RIGHT
/note="f1 ori"
/note="color: #ffff00"
promoter 6002..6106
/note="AmpR promoter"
/note="color: #ffffff; direction: RIGHT"
CDS 6107..6967
/codon_start=1
/note="AmpR"
/note="This feature has 2 segments:
1: 6107 .. 6175 / #ccffcc / signal sequence
2: 6176 .. 6967 / #ccffcc"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 7138..7726
/direction=RIGHT
/note="ori"
/note="color: #ffff00"