pAAV-shRNA-GFP-hGH-amp
| Vector Name | pAAV-shRNA-GFP-hGH-amp |
| VectorType | AAV Plasmid |
| Antibiotic Information | Amp/Puro
|
| Sequencing Primers | U6 promoter 5’--TACGTCCAAGGTCGGGCAGGAAGA--3’ |
| Additional Information | No Additional Information |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
Sequence 5783 BP; 1302 A; 1594 C; 1530 G; 1315 T; 42 other;
cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt 60
ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 120
aggggttcct gcggccgccc taggtacgtc caaggtcggg caggaagagg gcctatttcc 180
catgattcct tcatatttgc atatacgata caaggctgtt agagagataa ttagaattaa 240
tttgactgta aacacaaaga tattagtaca aaatacgtga cgtagaaagt aataatttct 300
tgggtagttt gcagttttaa aattatgttt taaaatggac tatcatatgc ttaccgtaac 360
ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacaAA AANNNNNNNN 420
NNNNNNNNNN NNNCGAANNN NNNNNNNNNN NNNNNNNNTT TTTTGGTACC AATTgtagta 480
atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac 540
ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac 600
gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt 660
acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat 720
tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga 780
ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt 840
ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca 900
ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg 960
tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta 1020
tataagcaga gctggtttag tgaaccgtca gggtaccgga tcccttaagt gaacaactag 1080
tgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga 1140
gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc 1200
cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg 1260
gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca 1320
catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac 1380
catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga 1440
caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct 1500
ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca 1560
gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca 1620
gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga 1680
caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca 1740
catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta 1800
caaggaattc ggaagcgggc agtgcaccaa ctacgccctg ctgaagctgg ccggcgacgt 1860
ggagagcaac cccggccccg gatccatggc caccgagtac aagcccacgg tgcgcctcgc 1920
cacccgcgac gacgtccccc gggccgtacg caccctcgcc gccgcgttcg ccgactaccc 1980
cgccacgcgc cacaccgtcg acccggaccg ccacatcgag cgggtcaccg agctgcaaga 2040
actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc 2100
cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat 2160
cggctcgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg 2220
cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc 2280
gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc 2340
cgagcgcgct ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta 2400
cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg 2460
gtgcatgacc cgcaagcccg gtgcctgaac gcgtgggtgg catccctgtg acccctcccc 2520
agtgcctctc ctggccctgg aagttgccac tccagtgccc accagccttg tcctaataaa 2580
attaagttgc atcattttgt ctgactaggt gtccttctat aatattatgg ggtggagggg 2640
ggtggtatgg agcaaggggc aagttgggaa gagaacctgt agggcctgcg gggtctattg 2700
ggaaccaagc tggagtgcag tggcacaatc ttggctcact gcaatctccg cctcctgggt 2760
tcaagcgatt ctcctgcctc agcctcccga gttgttggga ttccaggcat gcatgaccag 2820
gctcagctaa tttttgtttt tttggtagag acggggtttc accatattgg ccaggctggt 2880
ctccaactcc taatctcagg tgatctaccc accttggcct cccaaattgc tgggattaca 2940
ggcgtgaacc actgctccct tccctgtcct tcacgtgcgg accgagcggc cgcaggaacc 3000
cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg 3060
accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg 3120
cagctgcctg caggggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc 3180
acaccgcata cgtcaaagca accatagtac gcgccctgta gcggcgcatt aagcgcggcg 3240
ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccttagc gcccgctcct 3300
ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat 3360
cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt 3420
gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg 3480
acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac 3540
tctatctcgg gctattcttt tgatttataa gggattttgc cgatttcggt ctattggtta 3600
aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca 3660
attttatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga 3720
cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac 3780
agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg 3840
aaacgcgcga gccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc 3900
gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt 3960
ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt 4020
tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca 4080
ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt 4140
ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga 4200
tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa 4260
gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct 4320
gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat 4380
acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga 4440
tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc 4500
caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat 4560
gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa 4620
cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac 4680
tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa 4740
agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc 4800
tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc 4860
ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag 4920
acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta 4980
ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa 5040
gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc 5100
gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat 5160
ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga 5220
gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt 5280
tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata 5340
cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac 5400
cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg 5460
ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg 5520
tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag 5580
cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct 5640
ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc 5700
aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt 5760
ttgctggcct tttgctcaca tgt 5783
Remove "N" with shRNA sense and antisense sequences for full vector sequence.