pAAV-G-CMV-amp

Vector Name	pAAV-G-CMV-amp
VectorType	AAV Plasmid
Antibiotic Information	Ampicillin
Sequencing Primers	CMV-F: CGCAAATGGGCGGTAGGCGTG
Additional Information	No Additional Information

***Note: Features may not be to scale, please refer to sequence or text map for detailed information.

***Note: The vector sequence shown below is representative of the parental vector only and does not include the gene of interest. The sequence of the gene can be found through the accession number link on the product page. The restriction sites within the cloning region may differ from the provided sequence.


1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtgtct agggttccgc gttacataac ttacggtaaa
181 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
241 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
301 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
361 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
421 tacttggcag tacatctacg tttagtcatc gctattacca tggtgatgcg gttttggcag
481 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
541 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
601 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
661 agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc
721 catagaagaa ccgagtttaa actccctatc agtgatagag atctccctat cagtgataga
781 gagctagcga tatcaacaag tttgtacaaa aaagctgaac gagaaacgta aaatgatata
841 aatatcaata tattaaatta gattttgcat aaaaaacaga ctacataata ctgtaaaaca
901 caacatatcc agtcactatg gcggccgcat taggcacccc aggctttaca ctttatgctt
961 ccggctcgta taatgtgtgg attttgagtt aggatccgtc gagattttca ggagctaagg
1021 aagctaaaat ggagaaaaaa atcactggat ataccaccgt tgatatatcc caatggcatc
1081 gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg tacctataac cagaccgttc
1141 agctggatat tacggccttt ttaaagaccg taaagaaaaa taagcacaag ttttatccgg
1201 cctttattca cattcttgcc cgcctgatga atgctcatcc ggaattccgt atggcaatga
1261 aagacggtga gctggtgata tgggatagtg ttcacccttg ttacaccgtt ttccatgagc
1321 aaactgaaac gttttcatcg ctctggagtg aataccacga cgatttccgg cagtttctac
1381 acatatattc gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt
1441 ttattgagaa tatgtttttc gtctcagcca atccctgggt gagtttcacc agttttgatt
1501 taaacgtggc caatatggac aacttcttcg cccccgtttt caccatgggc aaatattata
1561 cgcaaggcga caaggtgctg atgccgctgg cgattcaggt tcatcatgcc gtttgtgatg
1621 gcttccatgt cggcagaatg cttaatgaat tacaacagta ctgcgatgag tggcagggcg
1681 gggcgtaaag atctggatcc ggcttactaa aagccagata acagtatgcg tatttgcgcg
1741 ctgatttttg cggtataaga atatatactg atatgtatac ccgaagtatg tcaaaaagag
1801 gtatgctatg aagcagcgta ttacagtgac agttgacagc gacagctatc agttgctcaa
1861 ggcatatatg atgtcaatat ctccggtctg gtaagcacaa ccatgcagaa tgaagcccgt
1921 cgtctgcgtg ccgaacgctg gaaagcggaa aatcaggaag ggatggctga ggtcgcccgg
1981 tttattgaaa tgaacggctc ttttgctgac gagaacaggg gctggtgaaa tgcagtttaa
2041 ggtttacacc tataaaagag agagccgtta tcgtctgttt gtggatgtac agagtgatat
2101 tattgacacg cccgggcgac ggatggtgat ccccctggcc agtgcacgtc tgctgtcaga
2161 taaagtctcc cgtgaacttt acccggtggt gcatatcggg gatgaaagct ggcgcatgat
2221 gaccaccgat atggccagtg tgccggtctc cgttatcggg gaagaagtgg ctgatctcag
2281 ccaccgcgaa aatgacatca aaaacgccat taacctgatg ttctggggaa tataaatgtc
2341 aggctccctt atacacagcc agtctgcagg tcgaccatag tgactggata tgttgtgttt
2401 tacagtatta tgtagtctgt tttttatgca aaatctaatt taatatattg atatttatat
2461 cattttacgt ttctcgttca gctttcttgt acaaagtggt tgatatctga ctcgagagat
2521 ctacgggtgg catccctgtg acccctcccc agtgcctctc ctggccctgg aagttgccac
2581 tccagtgccc accagccttg tcctaataaa attaagttgc atcattttgt ctgactaggt
2641 gtccttctat aatattatgg ggtggagggg ggtggtatgg agcaaggggc aagttgggaa
2701 gacaacctgt agggcctgcg gggtctattg ggaaccaagc tggagtgcag tggcacaatc
2761 ttggctcact gcaatctccg cctcctgggt tcaagcgatt ctcctgcctc agcctcccga
2821 gttgttggga ttccaggcat gcatgaccag gctcagctaa tttttgtttt tttggtagag
2881 acggggtttc accatattgg ccaggctggt ctccaactcc taatctcagg tgatctaccc
2941 accttggcct cccaaattgc tgggattaca ggcgtgaacc actgctccct tccctgtcct
3001 tctgattttg taggtaacca cgtgcggacc gagcggccgc aggaacccct agtgatggag
3061 ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc
3121 cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag
3181 gggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatacgt
3241 caaagcaacc atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
3301 cgcgcagcgt gaccgctaca cttgccagcg ccttagcgcc cgctcctttc gctttcttcc
3361 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
3421 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg
3481 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
3541 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaactct atctcgggct
3601 attcttttga tttataaggg attttgccga tttcggtcta ttggttaaaa aatgagctga
3661 tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt ttatggtgca
3721 ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac
3781 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
3841 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagcc
3901 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc
3961 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
4021 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
4081 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
4141 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
4201 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
4261 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
4321 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
4381 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
4441 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
4501 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
4561 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
4621 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
4681 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
4741 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
4801 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
4861 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
4921 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
4981 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
5041 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
5101 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
5161 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
5221 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
5281 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
5341 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
5401 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
5461 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
5521 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
5581 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
5641 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
5701 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
5761 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
5821 gctcacatgt

***Note: The text map shown below is representative of the parental vector only and does not include the gene of interest. The sequence of the gene can be found through the accession number link on the product page.

LOCUS Exported File 5830 bp ds-DNA circular SYN 09-DEC-2019
DEFINITION Double floxed Gi-coupled hM4D DREADD fused with mCherry under the 
control of human synapsin promoter..
ACCESSION .
VERSION .
KEYWORDS pAAV-G-CMV-amp
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5830)
AUTHORS Krashes MJ, Koda S, Ye C, Rogan SC, Adams AC, Cusher DS, 
Maratos-Flier E, Roth BL, Lowell BB
TITLE Rapid, reversible activation of AgRP neurons drives feeding behavior
in mice.
JOURNAL J Clin Invest. 2011 Apr;121(4):1424-8. doi: 10.1172/JCI46229.
PUBMED 21364278
REFERENCE 2 (bases 1 to 5830)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Dec-26-19 from SnapGene 1.1.3
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..5830
/organism="synthetic DNA construct"
/mol_type="other DNA"
repeat_region 1..130
/note="AAV2 ITR (alternate)"
/note="color: #ffcc99"
enhancer 160..462
/note="CMV enhancer"
/note="color: #ffffff"
promoter 463..666
/note="CMV promoter"
/note="color: #ffffff; direction: RIGHT"
protein_bind 743..761
/note="tet operator"
/note="color: #31849b"
protein_bind 764..782
/note="tet operator"
/note="color: #31849b"
protein_bind 796..920
/note="attR1"
/note="color: #31849b; direction: RIGHT"
promoter 945..975
/note="lac UV5 promoter"
/note="This forward directional feature has 3 segments:
   1: 945 .. 950 / #ffffff / -35
   2: 951 .. 968 / #ffffff
   3: 969 .. 975 / #ffffff / -10"
CDS 1029..1688
/codon_start=1
/note="CmR"
/note="color: #ccffcc"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
CDS 2030..2335
/codon_start=1
/note="ccdB"
/note="color: #993366"
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
protein_bind complement(2376..2500)
/note="attR2"
/note="color: #31849b; direction: LEFT"
polyA_signal 2525..3001
/note="hGH poly(A) signal"
/note="color: #a6acb3"
repeat_region 3041..3181
/note="AAV2 ITR"
/note="color: #ffe4c4"
repeat_region 3041..3170
/note="AAV2 ITR"
/note="color: #ffcc99"
rep_origin 3256..3711
/direction=RIGHT
/note="f1 ori"
/note="color: #ffff00"
promoter 4045..4149
/note="AmpR promoter"
/note="color: #ffffff; direction: RIGHT"
CDS 4150..5010
/codon_start=1
/note="AmpR"
/note="This feature has 2 segments:
   1: 4150 .. 4218 / #ccffcc / signal sequence
   2: 4219 .. 5010 / #ccffcc"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5181..5769
/direction=RIGHT
/note="ori"
/note="color: #ffff00"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtgtct agggttccgc gttacataac ttacggtaaa
181 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
241 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
301 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
361 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
421 tacttggcag tacatctacg tttagtcatc gctattacca tggtgatgcg gttttggcag
481 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
541 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
601 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
661 agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc
721 catagaagaa ccgagtttaa actccctatc agtgatagag atctccctat cagtgataga
781 gagctagcga tatcaacaag tttgtacaaa aaagctgaac gagaaacgta aaatgatata
841 aatatcaata tattaaatta gattttgcat aaaaaacaga ctacataata ctgtaaaaca
901 caacatatcc agtcactatg gcggccgcat taggcacccc aggctttaca ctttatgctt
961 ccggctcgta taatgtgtgg attttgagtt aggatccgtc gagattttca ggagctaagg
1021 aagctaaaat ggagaaaaaa atcactggat ataccaccgt tgatatatcc caatggcatc
1081 gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg tacctataac cagaccgttc
1141 agctggatat tacggccttt ttaaagaccg taaagaaaaa taagcacaag ttttatccgg
1201 cctttattca cattcttgcc cgcctgatga atgctcatcc ggaattccgt atggcaatga
1261 aagacggtga gctggtgata tgggatagtg ttcacccttg ttacaccgtt ttccatgagc
1321 aaactgaaac gttttcatcg ctctggagtg aataccacga cgatttccgg cagtttctac
1381 acatatattc gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt
1441 ttattgagaa tatgtttttc gtctcagcca atccctgggt gagtttcacc agttttgatt
1501 taaacgtggc caatatggac aacttcttcg cccccgtttt caccatgggc aaatattata
1561 cgcaaggcga caaggtgctg atgccgctgg cgattcaggt tcatcatgcc gtttgtgatg
1621 gcttccatgt cggcagaatg cttaatgaat tacaacagta ctgcgatgag tggcagggcg
1681 gggcgtaaag atctggatcc ggcttactaa aagccagata acagtatgcg tatttgcgcg
1741 ctgatttttg cggtataaga atatatactg atatgtatac ccgaagtatg tcaaaaagag
1801 gtatgctatg aagcagcgta ttacagtgac agttgacagc gacagctatc agttgctcaa
1861 ggcatatatg atgtcaatat ctccggtctg gtaagcacaa ccatgcagaa tgaagcccgt
1921 cgtctgcgtg ccgaacgctg gaaagcggaa aatcaggaag ggatggctga ggtcgcccgg
1981 tttattgaaa tgaacggctc ttttgctgac gagaacaggg gctggtgaaa tgcagtttaa
2041 ggtttacacc tataaaagag agagccgtta tcgtctgttt gtggatgtac agagtgatat
2101 tattgacacg cccgggcgac ggatggtgat ccccctggcc agtgcacgtc tgctgtcaga
2161 taaagtctcc cgtgaacttt acccggtggt gcatatcggg gatgaaagct ggcgcatgat
2221 gaccaccgat atggccagtg tgccggtctc cgttatcggg gaagaagtgg ctgatctcag
2281 ccaccgcgaa aatgacatca aaaacgccat taacctgatg ttctggggaa tataaatgtc
2341 aggctccctt atacacagcc agtctgcagg tcgaccatag tgactggata tgttgtgttt
2401 tacagtatta tgtagtctgt tttttatgca aaatctaatt taatatattg atatttatat
2461 cattttacgt ttctcgttca gctttcttgt acaaagtggt tgatatctga ctcgagagat
2521 ctacgggtgg catccctgtg acccctcccc agtgcctctc ctggccctgg aagttgccac
2581 tccagtgccc accagccttg tcctaataaa attaagttgc atcattttgt ctgactaggt
2641 gtccttctat aatattatgg ggtggagggg ggtggtatgg agcaaggggc aagttgggaa
2701 gacaacctgt agggcctgcg gggtctattg ggaaccaagc tggagtgcag tggcacaatc
2761 ttggctcact gcaatctccg cctcctgggt tcaagcgatt ctcctgcctc agcctcccga
2821 gttgttggga ttccaggcat gcatgaccag gctcagctaa tttttgtttt tttggtagag
2881 acggggtttc accatattgg ccaggctggt ctccaactcc taatctcagg tgatctaccc
2941 accttggcct cccaaattgc tgggattaca ggcgtgaacc actgctccct tccctgtcct
3001 tctgattttg taggtaacca cgtgcggacc gagcggccgc aggaacccct agtgatggag
3061 ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc
3121 cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag
3181 gggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatacgt
3241 caaagcaacc atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
3301 cgcgcagcgt gaccgctaca cttgccagcg ccttagcgcc cgctcctttc gctttcttcc
3361 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
3421 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg
3481 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
3541 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaactct atctcgggct
3601 attcttttga tttataaggg attttgccga tttcggtcta ttggttaaaa aatgagctga
3661 tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt ttatggtgca
3721 ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac
3781 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
3841 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagcc
3901 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc
3961 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
4021 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
4081 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
4141 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
4201 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
4261 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
4321 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
4381 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
4441 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
4501 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
4561 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
4621 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
4681 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
4741 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
4801 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
4861 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
4921 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
4981 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
5041 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
5101 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
5161 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
5221 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
5281 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
5341 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
5401 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
5461 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
5521 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
5581 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
5641 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
5701 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
5761 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
5821 gctcacatgt

                            LOCUS Exported File 5830 bp ds-DNA circular SYN 09-DEC-2019
DEFINITION Double floxed Gi-coupled hM4D DREADD fused with mCherry under the 
control of human synapsin promoter..
ACCESSION .
VERSION .
KEYWORDS pAAV-G-CMV-amp
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5830)
AUTHORS Krashes MJ, Koda S, Ye C, Rogan SC, Adams AC, Cusher DS, 
Maratos-Flier E, Roth BL, Lowell BB
TITLE Rapid, reversible activation of AgRP neurons drives feeding behavior
in mice.
JOURNAL J Clin Invest. 2011 Apr;121(4):1424-8. doi: 10.1172/JCI46229.
PUBMED 21364278
REFERENCE 2 (bases 1 to 5830)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Dec-26-19 from SnapGene 1.1.3
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..5830
/organism="synthetic DNA construct"
/mol_type="other DNA"
repeat_region 1..130
/note="AAV2 ITR (alternate)"
/note="color: #ffcc99"
enhancer 160..462
/note="CMV enhancer"
/note="color: #ffffff"
promoter 463..666
/note="CMV promoter"
/note="color: #ffffff; direction: RIGHT"
protein_bind 743..761
/note="tet operator"
/note="color: #31849b"
protein_bind 764..782
/note="tet operator"
/note="color: #31849b"
protein_bind 796..920
/note="attR1"
/note="color: #31849b; direction: RIGHT"
promoter 945..975
/note="lac UV5 promoter"
/note="This forward directional feature has 3 segments:
   1: 945 .. 950 / #ffffff / -35
   2: 951 .. 968 / #ffffff
   3: 969 .. 975 / #ffffff / -10"
CDS 1029..1688
/codon_start=1
/note="CmR"
/note="color: #ccffcc"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
CDS 2030..2335
/codon_start=1
/note="ccdB"
/note="color: #993366"
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
protein_bind complement(2376..2500)
/note="attR2"
/note="color: #31849b; direction: LEFT"
polyA_signal 2525..3001
/note="hGH poly(A) signal"
/note="color: #a6acb3"
repeat_region 3041..3181
/note="AAV2 ITR"
/note="color: #ffe4c4"
repeat_region 3041..3170
/note="AAV2 ITR"
/note="color: #ffcc99"
rep_origin 3256..3711
/direction=RIGHT
/note="f1 ori"
/note="color: #ffff00"
promoter 4045..4149
/note="AmpR promoter"
/note="color: #ffffff; direction: RIGHT"
CDS 4150..5010
/codon_start=1
/note="AmpR"
/note="This feature has 2 segments:
   1: 4150 .. 4218 / #ccffcc / signal sequence
   2: 4219 .. 5010 / #ccffcc"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5181..5769
/direction=RIGHT
/note="ori"
/note="color: #ffff00"