pAAV-G-PGK-hGH-amp
| Vector Name | pAAV-G-PGK-hGH-amp |
| VectorType | AAV Plasmid |
| Antibiotic Information | Ampicillin |
| Sequencing Primers | mPGK-Forward: 5'--CATTCTGCACGCTTCAAAAG--3' |
| Additional Information | No Additional Information |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgccc tagggggtag gggaggcgct tttcccaagg cagtctggag
181 catgcgcttt agcagccccg ctgggcactt ggcgctacac aagtggcctc tggcctcgca
241 cacattccac atccaccggt aggcgccaac cggctccgtt ctttggtggc cccttcgcgc
301 caccttctac tcctccccta gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca
361 ggacgtgaca aatggaagta gcacgtctca ctagtctcgt gcagatggac agcaccgctg
421 agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc tccttcgctt
481 tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc gggctcaggg
541 gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt caaaagcgca
601 cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcggagctc gtttagtgaa
661 ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa gaaccgagtt
721 taaactccct atcagtgata gagatctccc tatcagtgat agagagctag cgatatcaac
781 aagtttgtac aaaaaagctg aacgagaaac gtaaaatgat ataaatatca atatattaaa
841 ttagattttg cataaaaaac agactacata atactgtaaa acacaacata tccagtcact
901 atggcggccg cattaggcac cccaggcttt acactttatg cttccggctc gtataatgtg
961 tggattttga gttaggatcc gtcgagattt tcaggagcta aggaagctaa aatggagaaa
1021 aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga acattttgag
1081 gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga tattacggcc
1141 tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat tcacattctt
1201 gcccgcctga tgaatgctca tccggaattc cgtatggcaa tgaaagacgg tgagctggtg
1261 atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga aacgttttca
1321 tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata ttcgcaagat
1381 gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga gaatatgttt
1441 ttcgtctcag ccaatccctg ggtgagtttc accagttttg atttaaacgt ggccaatatg
1501 gacaacttct tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg
1561 ctgatgccgc tggcgattca ggttcatcat gccgtttgtg atggcttcca tgtcggcaga
1621 atgcttaatg aattacaaca gtactgcgat gagtggcagg gcggggcgta aagatctgga
1681 tccggcttac taaaagccag ataacagtat gcgtatttgc gcgctgattt ttgcggtata
1741 agaatatata ctgatatgta tacccgaagt atgtcaaaaa gaggtatgct atgaagcagc
1801 gtattacagt gacagttgac agcgacagct atcagttgct caaggcatat atgatgtcaa
1861 tatctccggt ctggtaagca caaccatgca gaatgaagcc cgtcgtctgc gtgccgaacg
1921 ctggaaagcg gaaaatcagg aagggatggc tgaggtcgcc cggtttattg aaatgaacgg
1981 ctcttttgct gacgagaaca ggggctggtg aaatgcagtt taaggtttac acctataaaa
2041 gagagagccg ttatcgtctg tttgtggatg tacagagtga tattattgac acgcccgggc
2101 gacggatggt gatccccctg gccagtgcac gtctgctgtc agataaagtc tcccgtgaac
2161 tttacccggt ggtgcatatc ggggatgaaa gctggcgcat gatgaccacc gatatggcca
2221 gtgtgccggt ctccgttatc ggggaagaag tggctgatct cagccaccgc gaaaatgaca
2281 tcaaaaacgc cattaacctg atgttctggg gaatataaat gtcaggctcc cttatacaca
2341 gccagtctgc aggtcgacca tagtgactgg atatgttgtg ttttacagta ttatgtagtc
2401 tgttttttat gcaaaatcta atttaatata ttgatattta tatcatttta cgtttctcgt
2461 tcagctttct tgtacaaagt ggttgatatc tgactcgagc caatcttaag gggtggcatc
2521 cctgtgaccc ctccccagtg cctctcctgg ccctggaagt tgccactcca gtgcccacca
2581 gccttgtcct aataaaatta agttgcatca ttttgtctga ctaggtgtcc ttctataata
2641 ttatggggtg gaggggggtg gtatggagca aggggcaagt tgggaagaca acctgtaggg
2701 cctgcggggt ctattgggaa ccaagctgga gtgcagtggc acaatcttgg ctcactgcaa
2761 tctccgcctc ctgggttcaa gcgattctcc tgcctcagcc tcccgagttg ttgggattcc
2821 aggcatgcat gaccaggctc agctaatttt tgtttttttg gtagagacgg ggtttcacca
2881 tattggccag gctggtctcc aactcctaat ctcaggtgat ctacccacct tggcctccca
2941 aattgctggg attacaggcg tgaaccactg ctcccttccc tgtccttcac gtgcggaccg
3001 agcggccgca ggaaccccta gtgatggagt tggccactcc ctctctgcgc gctcgctcgc
3061 tcactgaggc cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag
3121 tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc
3181 atctgtgcgg tatttcacac cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg
3241 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
3301 cttagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
3361 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
3421 cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac
3481 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
3541 tggaacaaca ctcaactcta tctcgggcta ttcttttgat ttataaggga ttttgccgat
3601 ttcggtctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
3661 aatattaacg tttacaattt tatggtgcac tctcagtaca atctgctctg atgccgcata
3721 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
3781 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
3841 ttcaccgtca tcaccgaaac gcgcgagccg ggagctgcat gtgtcagagg ttttcaccgt
3901 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
3961 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
4021 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
4081 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
4141 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
4201 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
4261 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
4321 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
4381 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
4441 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
4501 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
4561 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
4621 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
4681 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
4741 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
4801 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
4861 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
4921 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
4981 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
5041 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
5101 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
5161 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
5221 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
5281 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
5341 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
5401 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
5461 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
5521 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
5581 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
5641 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
5701 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
5761 tacggttcct ggccttttgc tggccttttg ctcacatgt
//
***Note: The text map shown below is representative of the parental
vector only and does not include the gene of interest. The sequence of the gene
can be found through the accession number link on the product page.
1 cctgcaggcagctgcgcgctcgctcgctcactgaggccgcccgggcgtcgggcgaccttt 60
GGACGTCCGTCGACGCGCGAGCGAGCGAGTGACTCCGGCGGGCCCGCAGCCCGCTGGAAA
61 ggtcgcccggcctcagtgagcgagcgagcgcgcagagagggagtggccaactccatcact 120
CCAGCGGGCCGGAGTCACTCGCTCGCTCGCGCGTCTCTCCCTCACCGGTTGAGGTAGTGA
121 aggggttcctgcggccgcCCTAGGgggtaggggaggcgcttttcccaaggcagtctggag 180
TCCCCAAGGACGCCGGCGGGATCCCCCATCCCCTCCGCGAAAAGGGTTCCGTCAGACCTC
181 catgcgctttagcagccccgctgggcacttggcgctacacaagtggcctctggcctcgca 240
GTACGCGAAATCGTCGGGGCGACCCGTGAACCGCGATGTGTTCACCGGAGACCGGAGCGT
241 cacattccacatccaccggtaggcgccaaccggctccgttctttggtggccccttcgcgc 300
GTGTAAGGTGTAGGTGGCCATCCGCGGTTGGCCGAGGCAAGAAACCACCGGGGAAGCGCG
301 caccttctactcctcccctagtcaggaagttcccccccgccccgcagctcgcgtcgtgca 360
GTGGAAGATGAGGAGGGGATCAGTCCTTCAAGGGGGGGCGGGGCGTCGAGCGCAGCACGT
361 ggacgtgacaaatggaagtagcacgtctcactagtctcgtgcagatggacagcaccgctg 420
CCTGCACTGTTTACCTTCATCGTGCAGAGTGATCAGAGCACGTCTACCTGTCGTGGCGAC
StuI
|
421 agcaatggaagcgggtaggcctttggggcagcggccaatagcagctttgctccttcgctt 480
TCGTTACCTTCGCCCATCCGGAAACCCCGTCGCCGGTTATCGTCGAAACGAGGAAGCGAA
481 tctgggctcagaggctgggaaggggtgggtccgggggcgggctcaggggcgggctcaggg 540
AGACCCGAGTCTCCGACCCTTCCCCACCCAGGCCCCCGCCCGAGTCCCCGCCCGAGTCCC
541 gcggggcgggcgcccgaaggtcctccggaggcccggcattctgcacgcttcaaaagcgca 600
CGCCCCGCCCGCGGGCTTCCAGGAGGCCTCCGGGCCGTAAGACGTGCGAAGTTTTCGCGT
SacI
|
601 cgtctgccgcgctgttctcctcttcctcatctccgggcctttcggagctcgtttagtgaa 660
GCAGACGGCGCGACAAGAGGAGAAGGAGTAGAGGCCCGGAAAGCCTCGAGCAAATCACTT
661 ccgtcagatcgcctggagacgccatccacgctgttttgacctccatagaagaaccgagtt 720
GGCAGTCTAGCGGACCTCTGCGGTAGGTGCGACAAAACTGGAGGTATCTTCTTGGCTCAA
tetO reg(726,765)>>> attR1 other(779,903)>>>
| |
721 taaactccctatcagtgatagagatctccctatcagtgatagagagctagcgatatcaac 780
ATTTGAGGGATAGTCACTATCTCTAGAGGGATAGTCACTATCTCTCGATCGCTATAGTTG
attR2 other(803,903)<<<
|
781 aagtttgtacaaaaaagctgaacgagaaacgtaaaatgatataaatatcaatatattaaa 840
TTCAAACATGTTTTTTCGACTTGCTCTTTGCATTTTACTATATTTATAGTTATATAATTT
841 ttagattttgcataaaaaacagactacataatactgtaaaacacaacatatccagtcact 900
AATCTAAAACGTATTTTTTGTCTGATGTATTATGACATTTTGTGTTGTATAGGTCAGTGA
901 atggcggccgcattaggcaccccaggctttacactttatgcttccggctcgtataatgtg 960
TACCGCCGGCGTAATCCGTGGGGTCCGAAATGTGAAATACGAAGGCCGAGCATATTACAC
ORF_1 rf(1)(1012,1671)>>>
|
CAT marker(1012,1671)>>>
|
961 tggattttgagttaggatccgtcgagattttcaggagctaaggaagctaaaatggagaaa 1020
ACCTAAAACTCAATCCTAGGCAGCTCTAAAAGTCCTCGATTCCTTCGATTTTACCTCTTT
1021 aaaatcactggatataccaccgttgatatatcccaatggcatcgtaaagaacattttgag 1080
TTTTAGTGACCTATATGGTGGCAACTATATAGGGTTACCGTAGCATTTCTTGTAAAACTC
1081 gcatttcagtcagttgctcaatgtacctataaccagaccgttcagctggatattacggcc 1140
CGTAAAGTCAGTCAACGAGTTACATGGATATTGGTCTGGCAAGTCGACCTATAATGCCGG
1141 tttttaaagaccgtaaagaaaaataagcacaagttttatccggcctttattcacattctt 1200
AAAAATTTCTGGCATTTCTTTTTATTCGTGTTCAAAATAGGCCGGAAATAAGTGTAAGAA
EcoRI
|
1201 gcccgcctgatgaatgctcatccggaattccgtatggcaatgaaagacggtgagctggtg 1260
CGGGCGGACTACTTACGAGTAGGCCTTAAGGCATACCGTTACTTTCTGCCACTCGACCAC
1261 atatgggatagtgttcacccttgttacaccgttttccatgagcaaactgaaacgttttca 1320
TATACCCTATCACAAGTGGGAACAATGTGGCAAAAGGTACTCGTTTGACTTTGCAAAAGT
1321 tcgctctggagtgaataccacgacgatttccggcagtttctacacatatattcgcaagat 1380
AGCGAGACCTCACTTATGGTGCTGCTAAAGGCCGTCAAAGATGTGTATATAAGCGTTCTA
1381 gtggcgtgttacggtgaaaacctggcctatttccctaaagggtttattgagaatatgttt 1440
CACCGCACAATGCCACTTTTGGACCGGATAAAGGGATTTCCCAAATAACTCTTATACAAA
1441 ttcgtctcagccaatccctgggtgagtttcaccagttttgatttaaacgtggccaatatg 1500
AAGCAGAGTCGGTTAGGGACCCACTCAAAGTGGTCAAAACTAAATTTGCACCGGTTATAC
NcoI
|
1501 gacaacttcttcgcccccgttttcaccatgggcaaatattatacgcaaggcgacaaggtg 1560
CTGTTGAAGAAGCGGGGGCAAAAGTGGTACCCGTTTATAATATGCGTTCCGCTGTTCCAC
1561 ctgatgccgctggcgattcaggttcatcatgccgtttgtgatggcttccatgtcggcaga 1620
GACTACGGCGACCGCTAAGTCCAAGTAGTACGGCAAACACTACCGAAGGTACAGCCGTCT
1621 atgcttaatgaattacaacagtactgcgatgagtggcagggcggggcgtaaagatctgga 1680
TACGAATTACTTAATGTTGTCATGACGCTACTCACCGTCCCGCCCCGCATTTCTAGACCT
1681 tccggcttactaaaagccagataacagtatgcgtatttgcgcgctgatttttgcggtata 1740
AGGCCGAATGATTTTCGGTCTATTGTCATACGCATAAACGCGCGACTAAAAACGCCATAT
1741 agaatatatactgatatgtatacccgaagtatgtcaaaaagaggtatgctatgaagcagc 1800
TCTTATATATGACTATACATATGGGCTTCATACAGTTTTTCTCCATACGATACTTCGTCG
1801 gtattacagtgacagttgacagcgacagctatcagttgctcaaggcatatatgatgtcaa 1860
CATAATGTCACTGTCAACTGTCGCTGTCGATAGTCAACGAGTTCCGTATATACTACAGTT
1861 tatctccggtctggtaagcacaaccatgcagaatgaagcccgtcgtctgcgtgccgaacg 1920
ATAGAGGCCAGACCATTCGTGTTGGTACGTCTTACTTCGGGCAGCAGACGCACGGCTTGC
1921 ctggaaagcggaaaatcaggaagggatggctgaggtcgcccggtttattgaaatgaacgg 1980
GACCTTTCGCCTTTTAGTCCTTCCCTACCGACTCCAGCGGGCCAAATAACTTTACTTGCC
ccdB other(2013,2318)>>>
|
1981 ctcttttgctgacgagaacaggggctggtgaaatgcagtttaaggtttacacctataaaa 2040
GAGAAAACGACTGCTCTTGTCCCCGACCACTTTACGTCAAATTCCAAATGTGGATATTTT
2041 gagagagccgttatcgtctgtttgtggatgtacagagtgatattattgacacgcccgggc 2100
CTCTCTCGGCAATAGCAGACAAACACCTACATGTCTCACTATAATAACTGTGCGGGCCCG
2101 gacggatggtgatccccctggccagtgcacgtctgctgtcagataaagtctcccgtgaac 2160
CTGCCTACCACTAGGGGGACCGGTCACGTGCAGACGACAGTCTATTTCAGAGGGCACTTG
2161 tttacccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgatatggcca 2220
AAATGGGCCACCACGTATAGCCCCTACTTTCGACCGCGTACTACTGGTGGCTATACCGGT
2221 gtgtgccggtctccgttatcggggaagaagtggctgatctcagccaccgcgaaaatgaca 2280
CACACGGCCAGAGGCAATAGCCCCTTCTTCACCGACTAGAGTCGGTGGCGCTTTTACTGT
2281 tcaaaaacgccattaacctgatgttctggggaatataaatgtcaggctcccttatacaca 2340
AGTTTTTGCGGTAATTGGACTACAAGACCCCTTATATTTACAGTCCGAGGGAATATGTGT
attR1 other(2359,2478)<<<
|
attR2 other(2359,2459)>>>
|
2341 gccagtctgcaggtcgaccatagtgactggatatgttgtgttttacagtattatgtagtc 2400
CGGTCAGACGTCCAGCTGGTATCACTGACCTATACAACACAAAATGTCATAATACATCAG
2401 tgttttttatgcaaaatctaatttaatatattgatatttatatcattttacgtttctcgt 2460
ACAAAAAATACGTTTTAGATTAAATTATATAACTATAAATATAGTAAAATGCAAAGAGCA
XhoI hGH_PA term(2511,2987)>>>
| |
2461 tcagctttcttgtacaaagtggttgatatctgactcgagccaatCTTAAGgggtggcatc 2520
AGTCGAAAGAACATGTTTCACCAACTATAGACTGAGCTCGGTTAGAATTCCCCACCGTAG
2521 cctgtgacccctccccagtgcctctcctggccctggaagttgccactccagtgcccacca 2580
GGACACTGGGGAGGGGTCACGGAGAGGACCGGGACCTTCAACGGTGAGGTCACGGGTGGT
2581 gccttgtcctaataaaattaagttgcatcattttgtctgactaggtgtccttctataata 2640
CGGAACAGGATTATTTTAATTCAACGTAGTAAAACAGACTGATCCACAGGAAGATATTAT
2641 ttatggggtggaggggggtggtatggagcaaggggcaagttgggaagacaacctgtaggg 2700
AATACCCCACCTCCCCCCACCATACCTCGTTCCCCGTTCAACCCTTCTGTTGGACATCCC
2701 cctgcggggtctattgggaaccaagctggagtgcagtggcacaatcttggctcactgcaa 2760
GGACGCCCCAGATAACCCTTGGTTCGACCTCACGTCACCGTGTTAGAACCGAGTGACGTT
2761 tctccgcctcctgggttcaagcgattctcctgcctcagcctcccgagttgttgggattcc 2820
AGAGGCGGAGGACCCAAGTTCGCTAAGAGGACGGAGTCGGAGGGCTCAACAACCCTAAGG
2821 aggcatgcatgaccaggctcagctaatttttgtttttttggtagagacggggtttcacca 2880
TCCGTACGTACTGGTCCGAGTCGATTAAAAACAAAAAAACCATCTCTGCCCCAAAGTGGT
2881 tattggccaggctggtctccaactcctaatctcaggtgatctacccaccttggcctccca 2940
ATAACCGGTCCGACCAGAGGTTGAGGATTAGAGTCCACTAGATGGGTGGAACCGGAGGGT
2941 aattgctgggattacaggcgtgaaccactgctcccttccctgtccttcacgtgcggaccg 3000
TTAACGACCCTAATGTCCGCACTTGGTGACGAGGGAAGGGACAGGAAGTGCACGCCTGGC
3001 agcggccgcaggaacccctagtgatggagttggccactccctctctgcgcgctcgctcgc 3060
TCGCCGGCGTCCTTGGGGATCACTACCTCAACCGGTGAGGGAGAGACGCGCGAGCGAGCG
3061 tcactgaggccgggcgaccaaaggtcgcccgacgcccgggctttgcccgggcggcctcag 3120
AGTGACTCCGGCCCGCTGGTTTCCAGCGGGCTGCGGGCCCGAAACGGGCCCGCCGGAGTC
3121 tgagcgagcgagcgcgcagctgcctgcaggggcgcctgatgcggtattttctccttacgc 3180
ACTCGCTCGCTCGCGCGTCGACGGACGTCCCCGCGGACTACGCCATAAAAGAGGAATGCG
3181 atctgtgcggtatttcacaccgcatacgtcaaagcaaccatagtacgcgccctgtagcgg 3240
TAGACACGCCATAAAGTGTGGCGTATGCAGTTTCGTTGGTATCATGCGCGGGACATCGCC
f1 origin(3242,3548)>>>
|
3241 cgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgc 3300
GCGTAATTCGCGCCGCCCACACCACCAATGCGCGTCGCACTGGCGATGTGAACGGTCGCG
3301 cttagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttcc 3360
GAATCGCGGGCGAGGAAAGCGAAAGAAGGGAAGGAAAGAGCGGTGCAAGCGGCCGAAAGG
3361 ccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacct 3420
GGCAGTTCGAGATTTAGCCCCCGAGGGAAATCCCAAGGCTAAATCACGAAATGCCGTGGA
3421 cgaccccaaaaaacttgatttgggtgatggttcacgtagtgggccatcgccctgatagac 3480
GCTGGGGTTTTTTGAACTAAACCCACTACCAAGTGCATCACCCGGTAGCGGGACTATCTG
3481 ggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaac 3540
CCAAAAAGCGGGAAACTGCAACCTCAGGTGCAAGAAATTATCACCTGAGAACAAGGTTTG
3541 tggaacaacactcaactctatctcgggctattcttttgatttataagggattttgccgat 3600
ACCTTGTTGTGAGTTGAGATAGAGCCCGATAAGAAAACTAAATATTCCCTAAAACGGCTA
3601 ttcggtctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaa 3660
AAGCCAGATAACCAATTTTTTACTCGACTAAATTGTTTTTAAATTGCGCTTAAAATTGTT
3661 aatattaacgtttacaattttatggtgcactctcagtacaatctgctctgatgccgcata 3720
TTATAATTGCAAATGTTAAAATACCACGTGAGAGTCATGTTAGACGAGACTACGGCGTAT
3721 gttaagccagccccgacacccgccaacacccgctgacgcgccctgacgggcttgtctgct 3780
CAATTCGGTCGGGGCTGTGGGCGGTTGTGGGCGACTGCGCGGGACTGCCCGAACAGACGA
3781 cccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggtt 3840
GGGCCGTAGGCGAATGTCTGTTCGACACTGGCAGAGGCCCTCGACGTACACAGTCTCCAA
3841 ttcaccgtcatcaccgaaacgcgcgagccgggagctgcatgtgtcagaggttttcaccgt 3900
AAGTGGCAGTAGTGGCTTTGCGCGCTCGGCCCTCGACGTACACAGTCTCCAAAAGTGGCA
3901 catcaccgaaacgcgcgagacgaaagggcctcgtgatacgcctatttttataggttaatg 3960
GTAGTGGCTTTGCGCGCTCTGCTTTCCCGGAGCACTATGCGGATAAAAATATCCAATTAC
3961 tcatgataataatggtttcttagacgtcaggtggcacttttcggggaaatgtgcgcggaa 4020
AGTACTATTATTACCAAAGAATCTGCAGTCCACCGTGAAAAGCCCCTTTACACGCGCCTT
amp prom(4049,4077)>>>
|
4021 cccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataac 4080
GGGGATAAACAAATAAAAAGATTTATGTAAGTTTATACATAGGCGAGTACTCTGTTATTG
ORF_2 rf(3)(4119,4979)>>>
|
amp marker(4119,4979)>>>
|
4081 cctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtg 4140
GGACTATTTACGAAGTTATTATAACTTTTTCCTTCTCATACTCATAAGTTGTAAAGGCAC
4141 tcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgc 4200
AGCGGGAATAAGGGAAAAAACGCCGTAAAACGGAAGGACAAAAACGAGTGGGTCTTTGCG
4201 tggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactgg 4260
ACCACTTTCATTTTCTACGACTTCTAGTCAACCCACGTGCTCACCCAATGTAGCTTGACC
4261 atctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatga 4320
TAGAGTTGTCGCCATTCTAGGAACTCTCAAAAGCGGGGCTTCTTGCAAAAGGTTACTACT
4321 gcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagc 4380
CGTGAAAATTTCAAGACGATACACCGCGCCATAATAGGGCATAACTGCGGCCCGTTCTCG
4381 aactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacag 4440
TTGAGCCAGCGGCGTATGTGATAAGAGTCTTACTGAACCAACTCATGAGTGGTCAGTGTC
4441 aaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatga 4500
TTTTCGTAGAATGCCTACCGTACTGTCATTCTCTTAATACGTCACGACGGTATTGGTACT
4501 gtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccg 4560
CACTATTGTGACGCCGGTTGAATGAAGACTGTTGCTAGCCTCCTGGCTTCCTCGATTGGC
4561 cttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctga 4620
GAAAAAACGTGTTGTACCCCCTAGTACATTGAGCGGAACTAGCAACCCTTGGCCTCGACT
4621 atgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgt 4680
TACTTCGGTATGGTTTGCTGCTCGCACTGTGGTGCTACGGACATCGTTACCGTTGTTGCA
4681 tgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagact 4740
ACGCGTTTGATAATTGACCGCTTGATGAATGAGATCGAAGGGCCGTTGTTAATTATCTGA
4741 ggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggt 4800
CCTACCTCCGCCTATTTCAACGTCCTGGTGAAGACGCGAGCCGGGAAGGCCGACCGACCA
4801 ttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactgg 4860
AATAACGACTATTTAGACCTCGGCCACTCGCACCCAGAGCGCCATAGTAACGTCGTGACC
4861 ggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaacta 4920
CCGGTCTACCATTCGGGAGGGCATAGCATCAATAGATGTGCTGCCCCTCAGTCCGTTGAT
4921 tggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaac 4980
ACCTACTTGCTTTATCTGTCTAGCGACTCTATCCACGGAGTGACTAATTCGTAACCATTG
4981 tgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaattta 5040
ACAGTCTGGTTCAAATGAGTATATATGAAATCTAACTAAATTTTGAAGTAAAAATTAAAT
5041 aaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagt 5100
TTTCCTAGATCCACTTCTAGGAAAAACTATTAGAGTACTGGTTTTAGGGAATTGCACTCA
pBR322 origin(5134,5753)>>>
|
5101 tttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctt 5160
AAAGCAAGGTGACTCGCAGTCTGGGGCATCTTTTCTAGTTTCCTAGAAGAACTCTAGGAA
5161 tttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggttt 5220
AAAAAGACGCGCATTAGACGACGAACGTTTGTTTTTTTGGTGGCGATGGTCGCCACCAAA
5221 gtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgc 5280
CAAACGGCCTAGTTCTCGATGGTTGAGAAAAAGGCTTCCATTGACCGAAGTCGTCTCGCG
5281 agataccaaatactgttcttctagtgtagccgtagttaggccaccacttcaagaactctg 5340
TCTATGGTTTATGACAAGAAGATCACATCGGCATCAATCCGGTGGTGAAGTTCTTGAGAC
5341 tagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcg 5400
ATCGTGGCGGATGTATGGAGCGAGACGATTAGGACAATGGTCACCGACGACGGTCACCGC
5401 ataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggt 5460
TATTCAGCACAGAATGGCCCAACCTGAGTTCTGCTATCAATGGCCTATTCCGCGTCGCCA
5461 cgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaac 5520
GCCCGACTTGCCCCCCAAGCACGTGTGTCGGGTCGAACCTCGCTTGCTGGATGTGGCTTG
5521 tgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcgg 5580
ACTCTATGGATGTCGCACTCGATACTCTTTCGCGGTGCGAAGGGCTTCCCTCTTTCCGCC
5581 acaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggg 5640
TGTCCATAGGCCATTCGCCGTCCCAGCCTTGTCCTCTCGCGTGCTCCCTCGAAGGTCCCC
5641 gaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgat 5700
CTTTGCGGACCATAGAAATATCAGGACAGCCCAAAGCGGTGGAGACTGAACTCGCAGCTA
5701 ttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttt 5760
AAAACACTACGAGCAGTCCCCCCGCCTCGGATACCTTTTTGCGGTCGTTGCGCCGGAAAA
5761 tacggttcctggccttttgctggccttttgctcacatgt 5799
ATGCCAAGGACCGGAAAACGACCGGAAAACGAGTGTACA
LOCUS dna 5799 bp
FEATURES Location/Qualifiers
misc_binding 437..442
/dbxref="REBASE:StuI"
misc_binding 645..650
/dbxref="REBASE:SacI"
Regulatory_Seq 726..765
/gene="tetO reg"
Other Gene 779..903
/gene="attR1 other"
Other Gene 803..903
/gene="attR2 other"
Marker 1012..1671
/gene="CAT marker"
ORF 1012..1671
/sequence="ORF_1 rf(1)"
misc_binding 1225..1230
/dbxref="REBASE:EcoRI"
misc_binding 1526..1531
/dbxref="REBASE:NcoI"
Other Gene 2013..2318
/gene="ccdB other"
Other Gene 2359..2478
/gene="attR1 other"
Other Gene 2359..2459
/gene="attR2 other"
misc_binding 2494..2499
/dbxref="REBASE:XhoI"
Terminator 2511..2987
/gene="hGH_PA term"
Rep_Origin 3242..3548
/gene="f1 origin"
Promoter 4049..4077
/gene="amp prom"
Marker 4119..4979
/gene="amp marker"
ORF 4119..4979
/sequence="ORF_2 rf(3)"
Rep_Origin 5134..5753
/gene="pBR322 origin"
BASE COUNT 1377 a 1449 c 1485 g 1488 t 0 others