BlasTaq™ HotStart DNA Polymerase
Specifications
SKU | G595 | ||||||||||
Name | BlasTaq™ HotStart DNA Polymerase | ||||||||||
Unit | 400 rxn | ||||||||||
Description |
(Equivalent to Cat. No. G011, G039, G937.) Eliminate non-specific amplification and primer-dimer formation!BlasTaq™ HotStart DNA Polymerase is a strategically-engineered, next generation Taq Polymerase that has rapid extension rates, robust performance, and contains a proprietary antibody that blocks polymerase activity at low temperatures. HotStart allows for a convenient reaction set-up at room temperature without non-specific amplification and primer dimer formation. With specialized reaction conditions, this polymerase provides increased processivity, yields, and sensitivity, while shortening reaction times by up to 70%, compared to wild-type Taq DNA polymerase. During the initial denaturation step, the antibody dissociates from the DNA polymerase and restores enzyme activity. This feature significantly reduces non-specific product formation that would otherwise compete for reagent availability offering higher specificity and improved yield of PCR products. BlasTaq™ has 5’-3’ polymerase and 5’-3’ exonuclease activities, lacks 3’-5’ exonuclease activity, and produces 3’-dA-tailed amplicons. PCR products made with BlasTaq™ can be used with TA cloning vectors. Product Features:
1 Buffer contains 1.5 mM Mg2+.
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Storage Condition |
Store at -20°C. |
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