T4 DNA Ligase | Applied Biological Materials Inc.

T4 DNA Ligase

Cat. No.

G467

Unit

200 U (200 μl)

Price

$152.00

Specifications

SKU	G467
Name	T4 DNA Ligase
Unit	200 U (200 μl)
Description	T4 DNA Ligase catalyzes the formation of a phosphodiester bond between the proximal 3’- hydroxyl and 5’- phosphate ends of adjacent, nicked nucleotides of dsDNA, dsRNA or DNA-RNA hybrids. Known to proceed via an adenylated intermediate, this endergonic reaction can be utilized to join both cohesive-ended and blunt-ended termini.
Applications	• Cloning of restriction fragments • Attaching double-stranded oligonucleotide linkers or adaptors to DNA • Site-directed mutagenesis • Nick repair of duplex DNA, RNA or DNA-RNA hybrids • Self-circularization of linear DNA
Concentration	1 U/μl
Format	Enzyme supplied with 5X Reaction Buffer
Storage Condition	Store all components at -20°C.

Documents

MSDS

T4 DNA Ligase MSDS

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