Pro Ligation-Free Cloning Kit (20 reactions)
Cat. No.
E086
Unit
20 Reactions
Price
$131.00
Specifications
| SKU | E086 |
| Name | Pro Ligation-Free Cloning Kit (20 reactions) |
| Unit | 20 Reactions |
| Description |
The Pro Ligation-Free Cloning Kit allows for the robust assembly of multiple DNA fragments in one step. Using a ligation-independent cloning method, as many as six DNA fragments are assembled seamlessly without concern for restriction enzyme site availability. abm’s Pro Ligation-Free cloning is a simple yet highly versatile method to rapidly clone highly complex, multiple component constructs. Key Kit Features:
Testimonial: "I tried the molecular cloning of inserting two DNA fragments (2Kb & 0.5Kb) into a 4Kb backbone by using the kit. After the transformation, I got about 100 colonies, and when I picked 24 colonies for colony PCR, the bright band from gel running told me that I got 20 potential hits, which is awesome. I sent some plasmids for sequencing, the result I received just now unveiled they are carrying the exact right fragment insertion. Collectively, this is a powerful kit." - Jianfeng Lan, The Buck Institute |
| Applications |
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| Storage Condition |
Store at -20°C |
FAQs
| Do I need to purify the PCR product first before use with this kit? Or can I use low melting agarose for the PCR gel and just melt the band portion and use in the kit? | |
|
The kit can be used in either scenario.
1. The kit comes with Cloning Optimizer and Assembly Enhancer enzymes which allow the user to not have to do gel extraction/purification following the amplification of DNA fragments, as methylated template DNA and single stranded DNA (e.g. primers) will be removed. 2. If PCR reactions contain multiple non-specific bands, it is highly advisable to either optimize the PCR or perform gel extraction, as the contaminating bands will be present in the cloning reaction if not prior removed. |
References
- Huang, R., Ding, R., Liu, Y., Li, F., Zhang, Z., & Wang, S. A. 2022. GATA transcription factor WC2 regulates the biosynthesis of astaxanthin in yeast Xanthophyllomyces dendrorhous. Microbial Biotechnology, 15(10), 2578-2593.
- Hwang, B.-J., Gonzales, R., Corzine, S., Stenson, E., Pidugu, L., & Lu, A.-L. (2022). DNA binding by the Rad9A subunit of the Rad9-Rad1-Hus1 complex. PLOS ONE, 17(8), e0272645. https://doi.org/10.1371/journal.pone.0272645
- Li, L., Liu, W., Fan, N., Li, F., Huang, B., Liu, Q., ... & Wang, X. (2022). Scallop IKK1 Responds to Bacterial and Virus-Related Pathogen Stimulation and Interacts With MyD88 Adaptor of Toll-Like Receptor Pathway Signaling. Frontiers in Immunology, 13. https://doi.org/10.3389/fimmu.2022.869845
- Huang, B., Wu, Y., Ma, J., Yang, B., Sang, X., Chen, J., Liu, W., Li, F., Li, L., Wang, X., Dong, J., & Wang, X. (2022). The first identified invertebrate LGP2-like homolog gene in the Pacific oyster Crassostrea gigas. Fish & Shellfish Immunology, 128, 238–245. https://doi.org/10.1016/j.fsi.2022.08.005
- Sang, X., Liu, W., Li, F., Huang, B., Li, L., Wang, X., Dong, J., Ma, J., Chen, J., & Wang, X. (2022). Scallop RIG-I-like receptor 1 responses to polyinosinic:polycytidylic acid challenge and its interactions with the mitochondrial antiviral signaling protein. Fish & Shellfish Immunology, 124, 490–496. https://doi.org/10.1016/j.fsi.2022.04.042
- Li M., et al. 2022. A Chitin-Binding Protein Ultra-highly expressed in the Outer Fold of Mantle is Related to Shell Colour in Pacific Oyster Crassostrea gigas. https://doi.org/10.55277/researchhub.jg604pn4
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