PCR Sure™ Kit
Cat. No.
G065
Unit
8 x 10 reactions
Price
$125.00
Specifications
| SKU | G065 | ||||||||||||||||||
| Name | PCR Sure™ Kit | ||||||||||||||||||
| Unit | 8 x 10 reactions | ||||||||||||||||||
| Description |
It has been well established that many variables can affect a PCR including template structures, inhibitors, primer design, annealing temperature, and magnesium concentration. The PCR Sure™ Kit is developed to simplify the PCR optimization process. The system consists of multiple thermostable DNA polymerases pre-mixed with 8 optimized buffers in a 2X MasterMix format, saving a great amount of time in PCR set-up. To find the optimal conditions for your difficult PCR, all you need to do is mix templates, primers, and H2O with the PCR Sure™ 2X MasterMixes.
Additional Materials Required
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| Storage Condition |
Store at -20°C. |
Documents
MSDS
FAQs
| Will this work for on long template samples with 60% GC content? | |
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Yes, the PCR Sure Kit can resolve the PCR difficulties with these sort of templates. Please make sure to try the touch down protocol for a success rate.
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| The kit was supplied with a separate vial of MgCl<sub>2</sub>? Do I have to add that to the reaction? | |
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The MgCl2 is already incorporated into the reaction mix so MgCl2 does not need to be added. Very rarely, an occasional customer might want to increase the MgC12 concentration, so we included it as a courtesy.
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References
- Chaturvedi, , NK. et al. "Experimental validation of predicted subcellular localizations of human proteins" BMC research notes 7 (1):912 (2014). PubMed: 25510246.
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