saCas9 Null Mutant NLS Protein

Cat. No.

K147

Unit

32.5µg (250pmol/ 25µL)

Price

$162.00

Specifications

SKU	K147
Name	saCas9 Null Mutant NLS Protein
Unit	32.5µg (250pmol/ 25µL)
Caution	This product is distributed for laboratory research only. Not for diagnostic use.
Description	The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system is the latest RNA-guided, endonuclease tool in genome editing which allows for very specific genomic disruption and replacement. The saCas9 Null Mutant Protein is created by mutating both cleavage domains of the wild type saCas9. Such a saCas9 protein retains its ability to bind to genomic DNA through gRNA:genomic DNA base pairing, however, the saCas9 Null Mutant does not introduce any genome modifications. Therefore, this protein can provide a useful negative control for CRISPR experiments. In addition, binding of the Null Mutant can act as a roadblock to hinder transcription, thus offering a useful tool to achieve reversible knock-down of gene expression. The Cas9 nuclease from the bacteria Staphylococcus aureus, abbreviated saCas9, is gaining popularity as an alternative to spCas9 due to its relatively smaller size. The saCas9 PAM sequence is 5’-NNGRRN (preferably 5’-NNGRRT). saCas9 NLS Null Mutant contains a SV40 T antigen nuclear localization sequence (NLS) on the C-terminus of the protein.
Concentration	10 µM, 1.30 mg/ml
Format	Enzyme supplied with 10X Reaction Buffer
Storage Buffer	10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM DTT, 300 mM NaCl, and 50% (v/v) Glycerol.
Storage Condition	Store all components at -20°C.

Documents

MSDS

Cas9 Proteins MSDS

FAQs

	Does K147 contain a HIS-tag?
	Yes, it does contain a His-tag.

References

There are no references for this product yet!

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