RNase A
Specifications
SKU | G117 |
Name | RNase A |
Unit | 25 mg |
Description |
RNase A is pancreatic bovine endoribonuclease that cleaves single stranded RNA by hydrolysis at cytosine and uracil residues. RNase A is very useful for the removal of RNA from plasmid DNA preparations. |
Applications |
• RNA removal from plasmid DNA preparations |
Concentration | A stock solution is recommended to be prepared in water at 10 mg/ml. Final usage concentration should be determined by end user based on specific application and usage. |
Storage Condition |
Store all components at -20°C . |
Note |
This product is distributed for laboratory research only Caution: Not for diagnostic use. |
Documents
MSDS
FAQs
What is the medium of dissolving the powder (RNase)? | |
We dissolve RNAse A in ddH2O to make stock solution of 1mg/ml. We do not have a special protocol for dissolving RNAses in other buffers.
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After dissolving your RNase A (powder) in solution, should I boil it at 100 C for 15-20 minutes to inactivate DNase contamination? | |
In order to remove any remaining DNase activity in the solution without compromising RNase A activity, we would suggest boiling the solution for 15 – 20 minutes. This is sufficient for most downstream molecular biology work e.g. preparation of RNA free plasmid DNA.
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