Golgi Labeling Lentivirus (GTS)(GFP)
Cat. No.
LVP715
Unit
2 x 100µl
Price
$940.00
Specifications
| SKU | LVP715 |
| Name | Golgi Labeling Lentivirus (GTS)(GFP) |
| Unit | 2 x 100µl |
| Vector | pLenti-III-GFP-N |
| Description |
This Golgi Labeling Lentivirus can be used to easily label the golgi apparatus. The vector expresses a fluorescent protein fused to the C-terminus of the Golgi targeting sequence (GTS), which causes localization of proteins to the golgi. This lentivirus offers a simple method to visualize the golgi without antibodies or chemical dyes, and is available with GFP (green) or RFP (red) reporters. Combine with other organelle labeling lentiviruses for multiplexed labeling. |
| Applications |
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| Storage Condition |
For long term storage, it is recommended to store the viruses at -80°C in small aliquots to avoid repeated freeze-thaw cycles. |
| Shipping Conditions |
Shipped with dry ice. |
Documents
Supporting Protocol
FAQs
| How long after transduction can the infection efficiency be observed? | |
|
You can observe transduction efficiency from 48 hours up to 5 days after infection.
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| What are the primers to use for SV40T and SV40T tsA58 detection? | |
|
PCR primers:
SV40T Forward Primer Sequence
5’ AGCCTGTAGAACCAAACATT 3'
SV40T Reverse Primer Sequence
5’ CTGCTGACTCTCAACATTCT 3'
The two primers should amplify the region between 3677-4468bp, giving a 792bp fragment.
|
| What is the sequence of the SV40 large T antigen? | |
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This information can be accessed on this page by clicking on "pLenti-SV40-T" under vector map. The Large T antigen is at position 5079-5927.
|
| For G221 and LV620, what does the 'V12' in RasV12 mean? | |
|
The V12 means that amino acid # 12 is mutated from a Valine to a Glycine. Other than that, the sequence matches the coding region of HRAS perfectly (NM_005343).
|
| Where is the SV40T tsA58 gene sequence? | |
|
The SV40T tsA58 gene is located between 3138-5264bp, with the Alanine-to-Valine mutation at amino acid 438.
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References
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