DpnI | Applied Biological Materials Inc.

DpnI

Cat. No.

E026

Unit

2000 U (100 μl)

Price

$78.50

Specifications

SKU	E026
Name	DpnI
Unit	2000 U (100 μl)
Reaction Definition	One unit is defined as the amount of Dpn1 required to digest 1 µg of dam methylated pBR322 DNA in 1 hour at 37°C in a total reaction volume of 50 µl.
Description	The DpnI restriction enzyme digests DNA at G^meA↓TC sites, requiring N6-methylation of the adenine residue for activity. DNA purified from a dam⁺ E. coli strain will be a substrate for DpnI due to the adenine methylation. DpnI cleaves hemi-methylated dam sites 60X more slowly than fully methylated dam sites.
Note	Heat inactivation 65°C for 20 minutes.

Documents

MSDS

E026 MSDS

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