DNase I (RNase-Free) | Applied Biological Materials Inc.

DNase I (RNase-Free)

Cat. No.

E091

Unit

200 U (100 μl)

Price

$99.00

Specifications

SKU

E091

Name

DNase I (RNase-Free)

Unit

200 U (100 μl)

Description

DNase I is a non-specific endonuclease derived from bovine pancreas that catalyzes the cleavage of phosphodiester bonds in single/double-stranded DNA, chromatin, and RNA:DNA hybrids to generate di-and/or oligonucleotide (5’-phosphorylated and 3’-hydroxylated) end-products. This product is RNase free and can be used in RNA applications.

Product Component	Quantity
DNase I (RNase-Free)	200 U (100 μl)
10X DNase I Reaction Buffer	1.0 ml

Applications

• Removes DNA from protein preparations and RNA samples
• Mediates nick translation
• Generates random fragments for dideoxy sequencing
• Degrade template DNA following in vitro transcription
• Mediate DNase I foot-printing

Concentration

2 U/μl

Note

One unit is defined as the amount of DNase I that catalyzes the degradation of 1 μg of DNA in 10 minutes at 37 °C into tetranucleotides or smaller fragments.

This product should not be used in digestions longer than 15 minutes or at temperatures higher than 37°C. For non-RNAse-Free DNAse I, see Cat. No. G028.

This product is distributed for laboratory research only

Caution: Not for diagnostic use.

Documents

MSDS

DNase I (RNase-Free) MSDS

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