dCas9-KRAB Lentiviral Vector
Cat. No.
K203
Unit
10 µg
Price
$205.00
Specifications
| SKU | K203 |
| Name | dCas9-KRAB Lentiviral Vector |
| Unit | 10 µg |
| Caution | This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. |
| Vector | pLenti-EF1a-dCas9-KRAB |
| Description |
dCas9-KRAB is used for CRISPR gene repression (CRISPRi). The Cas9 Double Mutant (dCas9) has changes at amino acid positions D10A and H840A which completely inactivate both the nuclease and nickase activities. In this construct, dCas9 carries a C-terminal Krüppel associated box (KRAB) domain. By targeting dCas9-KRAB to a promoter region, users will be able to specifically repress expression of virtually any gene of interest. This dCas9-KRAB lentiviral vector may be used for either non-viral transfection, or packaged into lentivirus for stable integration of dCas9-KRAB into host cells. Use this dCas9-KRAB lentiviral vector in conjunction with a CRISPRi sgRNA lentiviral vector designed specifically for CRISPR repression. |
| Format | Vector |
| Shipping Conditions |
Shipped at ambient temperature |
Documents
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FAQs
| How long after transduction can the infection efficiency be observed? | |
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You can observe transduction efficiency from 48 hours up to 5 days after infection.
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| What are the primers to use for SV40T and SV40T tsA58 detection? | |
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PCR primers:
SV40T Forward Primer Sequence
5’ AGCCTGTAGAACCAAACATT 3'
SV40T Reverse Primer Sequence
5’ CTGCTGACTCTCAACATTCT 3'
The two primers should amplify the region between 3677-4468bp, giving a 792bp fragment.
|
| What is the sequence of the SV40 large T antigen? | |
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This information can be accessed on this page by clicking on "pLenti-SV40-T" under vector map. The Large T antigen is at position 5079-5927.
|
| For G221 and LV620, what does the 'V12' in RasV12 mean? | |
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The V12 means that amino acid # 12 is mutated from a Valine to a Glycine. Other than that, the sequence matches the coding region of HRAS perfectly (NM_005343).
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| Where is the SV40T tsA58 gene sequence? | |
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The SV40T tsA58 gene is located between 3138-5264bp, with the Alanine-to-Valine mutation at amino acid 438.
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References
- Dai, X., Chen, X., Hakizimana, O., & Mei, Y. "Genetic interactions between ANLN and KDR are prognostic for breast cancer survival" Oncology Reports. : (2019).
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