Cas9 Null Mutant Protein

Cat. No.

K140

Unit

40µg (250pmol/ 25µL)

Price

$142.00

Specifications

SKU	K140
Name	Cas9 Null Mutant Protein
Unit	40µg (250pmol/ 25µL)
Caution	This product is distributed for laboratory research only. Not for diagnostic use.
Description	The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system is the latest RNA-guided, endonuclease tool in genome editing which allows for very specific genomic disruption and replacement. The Cas9 Null Mutant Protein is created by mutating both cleavage domains of the wild type Cas9 (D10A and H840A). Such a Cas9 protein retains its ability to bind to genomic DNA through gRNA:genomic DNA base pairing, however, unlike Cas9 Nuclease and Cas9 Nickase, where permanent gene disruption can be achieved, the Cas9 Null Mutant does not introduce any genome modifications. Therefore, this protein can provide a useful negative control for CRISPR experiments. In addition, binding of the Null Mutant can act as a roadblock to hinder transcription, thus offering a useful tool to achieve reversible knock-down of gene expression. The Cas9 nuclease from the bacteria Streptococcus pyogenes, abbreviated spCas9, is the most commonly used Cas9 variant. The reason for spCas9 popularity is two-fold. First the spCas9 PAM sequence is 5’-NGG, which is highly abundant in the genome allowing virtually any gene to be targeted. The spCas9 enzyme also has on average a higher efficiency in vivo compared to other variants.
Concentration	10 µM, 1.60 mg/ml
Format	Enzyme supplied with 10X Reaction Buffer
Storage Buffer	10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM DTT, 300 mM NaCl, and 50% (v/v) Glycerol.
Storage Condition	Store all components at -20°C.

Documents

MSDS

Cas9 Proteins MSDS

Cas9 Null Mutant Protein Functional Test Data

FAQs

	Does this cas9 protein contain a HIS-tag?
	Yes, it does contain a His-tag.

	Does this cas9 protein contain a Nuclear Localization Signal?
	No, this Cas9 protein does not have a Nuclear Localization Signal. If NLS is desired, we would recommend our Cas9 Null Mutant NLS Protein (Cat#K042/K142). A full list of our Cas9 products can be found here: https://www.abmgood.com/CRISPR-Cas9-Nuclease-Nickase-Vectors-Viruses.html

	Does K140 contain a HIS-tag?
	Yes, it does contain a His-tag.

	Does this cas9 protein contain a Nuclear Localization Signal?
	No, this Cas9 protein does not have a Nuclear Localization Signal. If NLS is desired, we would recommend our Cas9 Null Mutant NLS Protein (Cat#K042/K142). A full list of our Cas9 products can be found here: https://www.abmgood.com/CRISPR-Cas9-Nuclease-Nickase-Vectors-Viruses.html

	What size is this protein?
	Approximately 160kDa

References

There are no references for this product yet!

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