Cas9 Nuclease Protein | Applied Biological Materials Inc.

Cas9 Nuclease Protein

Cat. No.

K108

Unit

40µg (250pmol/ 25µL)

Price

$84.00

Specifications

SKU	K108
Name	Cas9 Nuclease Protein
Unit	40µg (250pmol/ 25µL)
Caution	This product is distributed for laboratory research only. Not for diagnostic use.
Description	The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system is the latest RNA-guided, endonuclease tool in genome editing which allows for very specific genomic disruption and replacement. The Cas9 nuclease serves to unwind the genomic DNA duplex next to conserved protospacer adjacent motifs (PAMs) and homes in on its target sequence, which is recognized by a complementary single-guide RNA. The resulting double-stranded break gets repaired by the non-homologous end joining (NHEJ) pathway, leading to a disruption in the open reading frame of the targeted gene. Alternatively, by supplying a suitable repair template, virtually any desired point mutation can be introduced at the break point via homology-directed repair (HDR). The Cas9 nuclease from the bacteria Streptococcus pyogenes, abbreviated spCas9, is the most commonly used Cas9 variant. The reason for spCas9 popularity is two-fold. First the spCas9 PAM sequence is 5’-NGG, which is highly abundant in the genome allowing virtually any gene to be targeted. The spCas9 enzyme also has on average a higher efficiency in vivo compared to other variants.
Concentration	10 µM, 1.60 mg/ml
Format	Enzyme supplied with 10X Reaction Buffer
Storage Buffer	10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM DTT, 300 mM NaCl, and 50% (v/v) Glycerol.
Storage Condition	Store all components at -20°C.

Documents

MSDS

Cas9 Proteins MSDS

FAQs

	Does this cas9 nuclease protein contain a Nuclear Localization Signal?
	No, this Cas9 nuclease protein does not have a Nuclear Localization Signal and therefore represents the native form. If NLS is desired, we would recommend our Cas9 Nuclease NLS Protein (Cat#K030/K130). A full list of our Cas9 products can be found here: https://www.abmgood.com/CRISPR-Cas9-Nuclease-Nickase-Vectors-Viruses.html

	Does the cas9 nuclease protein contain a HIS-tag?
	Yes, it does contain a His-tag.

	Does K108 contain a HIS-tag?
	Yes, it does contain a His-tag.

	Does this cas9 nuclease protein contain a Nuclear Localization Signal?
	No, this Cas9 nuclease protein does not have a Nuclear Localization Signal and therefore represents the native form. If NLS is desired, we would recommend our Cas9 Nuclease NLS Protein (Cat#K030/K130). A full list of our Cas9 products can be found here: https://www.abmgood.com/CRISPR-Cas9-Nuclease-Nickase-Vectors-Viruses.html

References

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