Cas12a (asCpf1) Nuclease NLS Protein
Cat. No.
K188
Unit
380 μg (2.5 nmol/ 250µL)
Price
$365.00
Specifications
| SKU | K188 |
| Name | Cas12a (asCpf1) Nuclease NLS Protein |
| Unit | 380 μg (2.5 nmol/ 250µL) |
| Caution | This product is distributed for laboratory research only. Not for diagnostic use. |
| Description |
Using Cas12a (asCpf1) in your CRISPR experiment offers several advantages over other CRISPR-associated nucleases.
asCpf1 is from the bacteria Acidaminococcus. This protein contains a SV40 T antigen nuclear localization signal (NLS) on the N-terminus of the protein. If the cut caused by asCpf1 is repaired by non-homologous end joining (NHEJ), an indel may be formed that disrupts the open reading frame of the targeted gene, leading to gene knockout. Alternatively, by supplying a repair template, a sequence can be knocked in at the cleavage site via homology directed repair (HDR). |
| Concentration | 10 μM, 1.5 mg/ml |
| Format | Enzyme supplied with 10X Reaction Buffer. |
| Storage Buffer | 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM DTT, 300 mM NaCl, and 50% (v/v) Glycerol. |
| Storage Condition | Store all components at -20°C. |
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