Screen It™ CRISPR Cas9 Cleavage Detection Kit

Cat. No.

G990

Unit

100 Reactions

Price

$1500.00

Specifications

SKU

G990

Name

Screen It™ CRISPR Cas9 Cleavage Detection Kit

Unit

100 Reactions

Description

abm’s Screen It™ CRISPR Cas9 Cleavage Detection Kit is a robust and precise RNA-dependent assay used to identify successful insertions and/or deletions (indels) of genomic DNA following a CRISPR-Cas9 experiment. CRISPR-Cas9 can introduce indels on either one or both copies of a given mammalian gene. abm’s Screen It™ CRISPR Cas9 Cleavage Detection Kit can easily identify if a given clone is monoallelic (has mutations in one copy), biallelic (both copies mutated) or is unchanged wild-type which is considered a major screening advantage over the standard T7E1 Surveyor Assay. This kit uses PCR to first amplify the sgRNA target site from CRISPR edited cells; this amplicon is subsequently cleaved by a ribonucleoprotein (RNP) complex which generates a unique pattern that can be easily resolved on agarose gel and interpreted by the user. Using this kit after gene editing saves time and energy from the arduous screening process and provides clear genotyping results.

Product Component	Quantity
Cell Lysis Buffer	1.25 ml
Protein Degrader	100 µl
Scaffold Template and Primer Mix	100 μl
2X sgRNA Synthesis Buffer	100 μl
sgRNA Synthesis Enzyme Mix	50 μl
sgRNA Control Oligo	20 µl
Wild Type Control Primer and Template Mix	20 µl
Monoallelic Control Primer and Template Mix	20 µl
Biallelic Control Primer and Template Mix	20 µl
RNP Degrader	100 µl
spCas9 Nuclease Protein	250 µl
10X Cas9 Reaction Buffer	1.25 ml
MegaFi™ Pro Fidelity 2X MasterMix	2 x 1.25 ml

Additional Materials Required
Target-specific Primers (abm offers sgRNA PCR Primer Pair Design & Synthesis Service, Cat. No. C336)
sgRNA Target-specific Oligo (abm offers sgRNA Target-Specific Oligo Design & Synthesis Service, Cat. No. C337)
CRISPR-edited Cells
Wild-type Cells
Nuclease-free Water

Storage Condition

Store all components at -20°C.

Documents

Supporting Protocol

Screen It™ CRISPR Cas9 Cleavage Detection Kit Product Manual

MSDS

Screen It™ CRISPR Cas9 Cleavage Detection Kit MSDS

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