Custom CRISPR sgRNA Non-Viral Vectors
A non-viral vector from abm is the simplest approach to deliver sgRNAs into cells, ideal for expressing sgRNA in a transient manner to limit off-target effects. You can order custom sgRNA non-viral vectors to transfect into cells that already express the spCas9 nuclease, and thus recruit spCas9 to the target gene (check out abm’s Cas9 Expressing Stable Cell Lines). Alternatively, we can construct All-in-One non-viral vectors that express spCas9 along with the sgRNA, making it even simpler to deliver CRISPR/Cas9 technology right into the target cell. When the non-viral vector has done its work, it can be diluted out of the cell population by passaging; just monitor the GFP fluorescence signal until it is gone.
Available custom CRISPR Vectors and Viruses:
| AAV Vector /Virus |
Lentivector /Virus |
Non-Viral Vector |
Adenovirus | |
|---|---|---|---|---|
| sgRNA for spCas9 | Available | Available | Available | Available |
| sgRNA for saCas9 | Available | - | - | - |
| All-in-One with spCas9 | - | Available | Available | - |
| All-in-One with saCas9 | Available | - | - | - |
| All-in-One with FnCas12a (FnCpf1) | - | Available | - | - |
| Browse Collection | Browse Collection | Browse Collection | Browse Collection |
Additional Resources:
"Construct works beautifully, definitely will get more if needed."
Emily Chen, UCLA, CRISPR ConstructsService Details
| Service Name | Vector | Quantity | Cat. No. | Price |
|---|---|---|---|---|
| Custom CRISPR sgRNA Non-Viral Vector (for spCas9) – single target | pNV-sgRNA-GFP | 1.0 ug | C439 | $135.00 |
| Custom CRISPR sgRNA Non-Viral Vector (for spCas9) – set of three targets | pNV-sgRNA-GFP | 3 x 1.0 ug | C443 | $360.00 |
| Custom CRISPR All-in-One Non-Viral Vector (with spCas9) – single target | pNV-sgRNA-Cas9-2A-GFP | 1.0 ug | C440 | $125.00 |
| Custom CRISPR All-in-One Non-Viral Vector(with spCas9) – set of three targets | pNV-sgRNA-Cas9-2A-GFP | 3 x 1.0 ug | C444 | $355.00 |
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Citations
| 01 | Kang, Y. J. et al. "Regulation of NKT cell-mediated immune responses to tumours and liver inflammation by mitochondrial PGAM5-Drp1 signalling." Nat. Commun. (2015) 6:8371 doi: 10.1038/ncomms9371 |
| 02 | Jiang, G. et al. "Isorhapontigenin (ISO) inhibits invasive bladder cancer (BC) formation in vivo and human BC invasion in vitro by targeting STAT1/FOXO1 Axis." Cancer Prev Res. Published Online First April 14, 2016.doi: 10.1158/1940-6207.CAPR-15-0338 |
| 03 | Okugawa, Y. et al. "Clinical significance of SNORA42 as an oncogene and a prognostic biomarker in colorectal cancer." Gut (2015)doi:10.1136/gutjnl-2015-309359 |