Cas9 Nuclease Non-Viral Vector
Cat. No.
K095
Unit
10 μg
Price
$205.00
Specifications
| SKU | K095 |
| Name | Cas9 Nuclease Non-Viral Vector |
| Unit | 10 μg |
| Caution | This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. |
| Vector | pNV-EF1a-Cas9-2A-GFP |
| Description |
This Cas9 nuclease can be used with a target-specific sgRNA to bind DNA and create a double-stranded cut. It is provided in a non-viral vector (plasmid) for quick and easy transfection. Transfection will result in transient expression of Cas9, leading to reduced chances of off-target editing. The Cas9 provided in this non-viral vector is spCas9 from Streptococcus pyogenes. |
| Format | Plasmid |
| Shipping Conditions |
Shipped at ambient temperature. |
Documents
There are no Documents for this product yet!
FAQs
| How long after transduction can the infection efficiency be observed? | |
|
You can observe transduction efficiency from 48 hours up to 5 days after infection.
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| What are the primers to use for SV40T and SV40T tsA58 detection? | |
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PCR primers:
SV40T Forward Primer Sequence
5’ AGCCTGTAGAACCAAACATT 3'
SV40T Reverse Primer Sequence
5’ CTGCTGACTCTCAACATTCT 3'
The two primers should amplify the region between 3677-4468bp, giving a 792bp fragment.
|
| What is the sequence of the SV40 large T antigen? | |
|
This information can be accessed on this page by clicking on "pLenti-SV40-T" under vector map. The Large T antigen is at position 5079-5927.
|
| For G221 and LV620, what does the 'V12' in RasV12 mean? | |
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The V12 means that amino acid # 12 is mutated from a Valine to a Glycine. Other than that, the sequence matches the coding region of HRAS perfectly (NM_005343).
|
| Where is the SV40T tsA58 gene sequence? | |
|
The SV40T tsA58 gene is located between 3138-5264bp, with the Alanine-to-Valine mutation at amino acid 438.
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References
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