pNV-EF1a-Cas9-2A-GFP
| Vector Name | pNV-EF1a-Cas9-2A-GFP |
| VectorType | Non-Viral Vector |
| Antibiotic Information | Ampicillin |
| Additional Information | No Additional Information |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acctcgcgaa
421 tgcatctaga tatccctagg ggctccggtg cccgtcagtg ggcagagcgc acatcgccca
481 cagtccccga gaagttgggg ggaggggtcg gcaattgaac cggtgcctag agaaggtggc
541 gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg
601 gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg
661 ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg
721 gcccttgcgt gccttgaatt acttccactg gctgcagtac gtgattcttg atcccgagct
781 tcgggttgga agtgggtggg agagttcgag gccttgcgct taaggagccc cttcgcctcg
841 tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct
901 tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt taaaattttt gatgacctgc
961 tgcgacgctt tttttctggc aagatagtct tgtaaatgcg ggccaagatc tgcacactgg
1021 tatttcggtt tttggggccg cgggcggcga cggggcccgt gcgtcccagc gcacatgttc
1081 ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga cgggggtagt ctcaagctgg
1141 ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag
1201 gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc
1261 agggagctca aaatggagga cgcggcgctc gggagagcgg gcgggtgagt cacccacaca
1321 aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt gactccacgg agtaccgggc
1381 gccgtccagg cacctcgatt agttctcgag cttttggagt acgtcgtctt taggttgggg
1441 ggaggggttt tatgcgatgg agtttcccca cactgagtgg gtggagactg aagttaggcc
1501 agcttggcac ttgatgtaat tctccttgga atttgccctt tttgagtttg gatcttggtt
1561 cattctcaag cctcagacag tggttcaaag tttttttctt ccatttcagg tgtcgtgagc
1621 tagcacctcg ccatggcccc aaagaagaag cggaaggtcg gtatccacgg agtcccagca
1681 gccgacaaga agtacagcat cggcctggac atcggcacca actctgtggg ctgggccgtg
1741 atcaccgacg agtacaaggt gcccagcaag aaattcaagg tgctgggcaa caccgaccgg
1801 cacagcatca agaagaacct gatcggagcc ctgctgttcg acagcggcga aacagccgag
1861 gccacccggc tgaagagaac cgccagaaga agatacacca gacggaagaa ccggatctgc
1921 tatctgcaag agatcttcag caacgagatg gccaaggtgg acgacagctt cttccacaga
1981 ctggaagagt ccttcctggt ggaagaggat aagaagcacg agcggcaccc catcttcggc
2041 aacatcgtgg acgaggtggc ctaccacgag aagtacccca ccatctacca cctgagaaag
2101 aaactggtgg acagcaccga caaggccgac ctgcggctga tctatctggc cctggcccac
2161 atgatcaagt tccggggcca cttcctgatc gagggcgacc tgaaccccga caacagcgac
2221 gtggacaagc tgttcatcca gctggtgcag acctacaacc agctgttcga ggaaaacccc
2281 atcaacgcca gcggcgtgga cgccaaggcc atcctgtctg ccagactgag caagagcaga
2341 cggctggaaa atctgatcgc ccagctgccc ggcgagaaga agaatggcct gttcggcaac
2401 ctgattgccc tgagcctggg cctgaccccc aacttcaaga gcaacttcga cctggccgag
2461 gatgccaaac tgcagctgag caaggacacc tacgacgacg acctggacaa cctgctggcc
2521 cagatcggcg accagtacgc cgacctgttt ctggccgcca agaacctgtc cgacgccatc
2581 ctgctgagcg acatcctgag agtgaacacc gagatcacca aggcccccct gagcgcctct
2641 atgatcaaga gatacgacga gcaccaccag gacctgaccc tgctgaaagc tctcgtgcgg
2701 cagcagctgc ctgagaagta caaagagatt ttcttcgacc agagcaagaa cggctacgcc
2761 ggctacattg acggcggagc cagccaggaa gagttctaca agttcatcaa gcccatcctg
2821 gaaaagatgg acggcaccga ggaactgctc gtgaagctga acagagagga cctgctgcgg
2881 aagcagcgga ccttcgacaa cggcagcatc ccccaccaga tccacctggg agagctgcac
2941 gccattctgc ggcggcagga agatttttac ccattcctga aggacaaccg ggaaaagatc
3001 gagaagatcc tgaccttccg catcccctac tacgtgggcc ctctggccag gggaaacagc
3061 agattcgcct ggatgaccag aaagagcgag gaaaccatca ccccctggaa cttcgaggaa
3121 gtggtggaca agggcgcttc cgcccagagc ttcatcgagc ggatgaccaa cttcgataag
3181 aacctgccca acgagaaggt gctgcccaag cacagcctgc tgtacgagta cttcaccgtg
3241 tataacgagc tgaccaaagt gaaatacgtg accgagggaa tgagaaagcc cgccttcctg
3301 agcggcgagc agaaaaaggc catcgtggac ctgctgttca agaccaaccg gaaagtgacc
3361 gtgaagcagc tgaaagagga ctacttcaag aaaatcgagt gcttcgactc cgtggaaatc
3421 tccggcgtgg aagatcggtt caacgcctcc ctgggcacat accacgatct gctgaaaatt
3481 atcaaggaca aggacttcct ggacaatgag gaaaacgagg acattctgga agatatcgtg
3541 ctgaccctga cactgtttga ggacagagag atgatcgagg aacggctgaa aacctatgcc
3601 cacctgttcg acgacaaagt gatgaagcag ctgaagcggc ggagatacac cggctggggc
3661 aggctgagcc ggaagctgat caacggcatc cgggacaagc agtccggcaa gacaatcctg
3721 gatttcctga agtccgacgg cttcgccaac agaaacttca tgcagctgat ccacgacgac
3781 agcctgacct ttaaagagga catccagaaa gcccaggtgt ccggccaggg cgatagcctg
3841 cacgagcaca ttgccaatct ggccggcagc cccgccatta agaagggcat cctgcagaca
3901 gtgaaggtgg tggacgagct cgtgaaagtg atgggccggc acaagcccga gaacatcgtg
3961 atcgaaatgg ccagagagaa ccagaccacc cagaagggac agaagaacag ccgcgagaga
4021 atgaagcgga tcgaagaggg catcaaagag ctgggcagcc agatcctgaa agaacacccc
4081 gtggaaaaca cccagctgca gaacgagaag ctgtacctgt actacctgca gaatgggcgg
4141 gatatgtacg tggaccagga actggacatc aaccggctgt ccgactacga tgtggaccat
4201 atcgtgcctc agagctttct gaaggacgac tccatcgaca acaaggtgct gaccagaagc
4261 gacaagaacc ggggcaagag cgacaacgtg ccctccgaag aggtcgtgaa gaagatgaag
4321 aactactggc ggcagctgct gaacgccaag ctgattaccc agagaaagtt cgacaatctg
4381 accaaggccg agagaggcgg cctgagcgaa ctggataagg ccggcttcat caagagacag
4441 ctggtggaaa cccggcagat cacaaagcac gtggcacaga tcctggactc ccggatgaac
4501 actaagtacg acgagaatga caagctgatc cgggaagtga aagtgatcac cctgaagtcc
4561 aagctggtgt ccgatttccg gaaggatttc cagttttaca aagtgcgcga gatcaacaac
4621 taccaccacg cccacgacgc ctacctgaac gccgtcgtgg gaaccgccct gatcaaaaag
4681 taccctaagc tggaaagcga gttcgtgtac ggcgactaca aggtgtacga cgtgcggaag
4741 atgatcgcca agagcgagca ggaaatcggc aaggctaccg ccaagtactt cttctacagc
4801 aacatcatga actttttcaa gaccgagatt accctggcca acggcgagat ccggaagcgg
4861 cctctgatcg agacaaacgg cgaaaccggg gagatcgtgt gggataaggg ccgggatttt
4921 gccaccgtgc ggaaagtgct gagcatgccc caagtgaata tcgtgaaaaa gaccgaggtg
4981 cagacaggcg gcttcagcaa agagtctatc ctgcccaaga ggaacagcga taagctgatc
5041 gccagaaaga aggactggga ccctaagaag tacggcggct tcgacagccc caccgtggcc
5101 tattctgtgc tggtggtggc caaagtggaa aagggcaagt ccaagaaact gaagagtgtg
5161 aaagagctgc tggggatcac catcatggaa agaagcagct tcgagaagaa tcccatcgac
5221 tttctggaag ccaagggcta caaagaagtg aaaaaggacc tgatcatcaa gctgcctaag
5281 tactccctgt tcgagctgga aaacggccgg aagagaatgc tggcctctgc cggcgaactg
5341 cagaagggaa acgaactggc cctgccctcc aaatatgtga acttcctgta cctggccagc
5401 cactatgaga agctgaaggg ctcccccgag gataatgagc agaaacagct gtttgtggaa
5461 cagcacaagc actacctgga cgagatcatc gagcagatca gcgagttctc caagagagtg
5521 atcctggccg acgctaatct ggacaaagtg ctgtccgcct acaacaagca ccgggataag
5581 cccatcagag agcaggccga gaatatcatc cacctgttta ccctgaccaa tctgggagcc
5641 cctgccgcct tcaagtactt tgacaccacc atcgaccgga agaggtacac cagcaccaaa
5701 gaggtgctgg acgccaccct gatccaccag agcatcaccg gcctgtacga gacacggatc
5761 gacctgtctc agctgggagg cgacaaaagg ccggcggcca cgaaaaaggc cggccaggca
5821 aaaaagaaaa agacgcgtca gtgtactaat tatgctctct tgaaattggc tggagatgtt
5881 gagagcaacc caggtcccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
5941 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
6001 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
6061 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc
6121 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
6181 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
6241 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
6301 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
6361 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac
6421 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
6481 ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag
6541 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
6601 gacgagctgt acaagtaact cgagctgtgc cttctagttg ccagccatct gttgtttgcc
6661 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
6721 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
6781 ggcaggacag caagggggag gattgggaag agaatagcag gcatgctggg gaaagcttgg
6841 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
6901 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
6961 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
7021 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
7081 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
7141 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
7201 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
7261 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
7321 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
7381 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
7441 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
7501 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
7561 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
7621 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
7681 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
7741 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
7801 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
7861 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
7921 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
7981 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
8041 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
8101 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
8161 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
8221 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
8281 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
8341 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
8401 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
8461 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
8521 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
8581 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
8641 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
8701 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
8761 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
8821 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
8881 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
8941 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
9001 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
9061 ggccctttcg tc
LOCUS dna 9072 bp
FEATURES Location/Qualifiers
misc_binding 183..188
/dbxref="REBASE:NdeI"
Reporter 239..398
/gene="lacZ_a reporter"
misc_binding 396..401
/dbxref="REBASE:EcoRI"
misc_binding 408..413
/dbxref="REBASE:KpnI"
misc_binding 414..419
/dbxref="REBASE:NruI"
misc_binding 425..430
/dbxref="REBASE:XbaI"
ORF 426..1358
/sequence="ORF_3 rf(5)"
Promoter 441..1618
/gene="EF1a prom"
ORF 1606..4200
/sequence="ORF_2 rf(4)"
ORF 1633..6618
/sequence="ORF_1 rf(1)"
Reporter 5899..6615
/gene="EGFP reporter"
misc_binding 6833..6838
/dbxref="REBASE:HindIII"
Promoter 6900..6929
/gene="lac prom"
Rep_Origin 7238..7857
/gene="pBR322 origin"
Marker 8012..8872
/gene="amp marker"
ORF 8012..8872
/sequence="ORF_4 rf(6)"
Promoter 8914..8942
/gene="amp prom"
BASE COUNT 2296 a 2462 c 2548 g 1766 t 0 others
ORIGIN
1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acctcgcgaa
421 tgcatctaga tatccctagg ggctccggtg cccgtcagtg ggcagagcgc acatcgccca
481 cagtccccga gaagttgggg ggaggggtcg gcaattgaac cggtgcctag agaaggtggc
541 gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg
601 gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg
661 ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg
721 gcccttgcgt gccttgaatt acttccactg gctgcagtac gtgattcttg atcccgagct
781 tcgggttgga agtgggtggg agagttcgag gccttgcgct taaggagccc cttcgcctcg
841 tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct
901 tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt taaaattttt gatgacctgc
961 tgcgacgctt tttttctggc aagatagtct tgtaaatgcg ggccaagatc tgcacactgg
1021 tatttcggtt tttggggccg cgggcggcga cggggcccgt gcgtcccagc gcacatgttc
1081 ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga cgggggtagt ctcaagctgg
1141 ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag
1201 gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc
1261 agggagctca aaatggagga cgcggcgctc gggagagcgg gcgggtgagt cacccacaca
1321 aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt gactccacgg agtaccgggc
1381 gccgtccagg cacctcgatt agttctcgag cttttggagt acgtcgtctt taggttgggg
1441 ggaggggttt tatgcgatgg agtttcccca cactgagtgg gtggagactg aagttaggcc
1501 agcttggcac ttgatgtaat tctccttgga atttgccctt tttgagtttg gatcttggtt
1561 cattctcaag cctcagacag tggttcaaag tttttttctt ccatttcagg tgtcgtgagc
1621 tagcacctcg ccatggcccc aaagaagaag cggaaggtcg gtatccacgg agtcccagca
1681 gccgacaaga agtacagcat cggcctggac atcggcacca actctgtggg ctgggccgtg
1741 atcaccgacg agtacaaggt gcccagcaag aaattcaagg tgctgggcaa caccgaccgg
1801 cacagcatca agaagaacct gatcggagcc ctgctgttcg acagcggcga aacagccgag
1861 gccacccggc tgaagagaac cgccagaaga agatacacca gacggaagaa ccggatctgc
1921 tatctgcaag agatcttcag caacgagatg gccaaggtgg acgacagctt cttccacaga
1981 ctggaagagt ccttcctggt ggaagaggat aagaagcacg agcggcaccc catcttcggc
2041 aacatcgtgg acgaggtggc ctaccacgag aagtacccca ccatctacca cctgagaaag
2101 aaactggtgg acagcaccga caaggccgac ctgcggctga tctatctggc cctggcccac
2161 atgatcaagt tccggggcca cttcctgatc gagggcgacc tgaaccccga caacagcgac
2221 gtggacaagc tgttcatcca gctggtgcag acctacaacc agctgttcga ggaaaacccc
2281 atcaacgcca gcggcgtgga cgccaaggcc atcctgtctg ccagactgag caagagcaga
2341 cggctggaaa atctgatcgc ccagctgccc ggcgagaaga agaatggcct gttcggcaac
2401 ctgattgccc tgagcctggg cctgaccccc aacttcaaga gcaacttcga cctggccgag
2461 gatgccaaac tgcagctgag caaggacacc tacgacgacg acctggacaa cctgctggcc
2521 cagatcggcg accagtacgc cgacctgttt ctggccgcca agaacctgtc cgacgccatc
2581 ctgctgagcg acatcctgag agtgaacacc gagatcacca aggcccccct gagcgcctct
2641 atgatcaaga gatacgacga gcaccaccag gacctgaccc tgctgaaagc tctcgtgcgg
2701 cagcagctgc ctgagaagta caaagagatt ttcttcgacc agagcaagaa cggctacgcc
2761 ggctacattg acggcggagc cagccaggaa gagttctaca agttcatcaa gcccatcctg
2821 gaaaagatgg acggcaccga ggaactgctc gtgaagctga acagagagga cctgctgcgg
2881 aagcagcgga ccttcgacaa cggcagcatc ccccaccaga tccacctggg agagctgcac
2941 gccattctgc ggcggcagga agatttttac ccattcctga aggacaaccg ggaaaagatc
3001 gagaagatcc tgaccttccg catcccctac tacgtgggcc ctctggccag gggaaacagc
3061 agattcgcct ggatgaccag aaagagcgag gaaaccatca ccccctggaa cttcgaggaa
3121 gtggtggaca agggcgcttc cgcccagagc ttcatcgagc ggatgaccaa cttcgataag
3181 aacctgccca acgagaaggt gctgcccaag cacagcctgc tgtacgagta cttcaccgtg
3241 tataacgagc tgaccaaagt gaaatacgtg accgagggaa tgagaaagcc cgccttcctg
3301 agcggcgagc agaaaaaggc catcgtggac ctgctgttca agaccaaccg gaaagtgacc
3361 gtgaagcagc tgaaagagga ctacttcaag aaaatcgagt gcttcgactc cgtggaaatc
3421 tccggcgtgg aagatcggtt caacgcctcc ctgggcacat accacgatct gctgaaaatt
3481 atcaaggaca aggacttcct ggacaatgag gaaaacgagg acattctgga agatatcgtg
3541 ctgaccctga cactgtttga ggacagagag atgatcgagg aacggctgaa aacctatgcc
3601 cacctgttcg acgacaaagt gatgaagcag ctgaagcggc ggagatacac cggctggggc
3661 aggctgagcc ggaagctgat caacggcatc cgggacaagc agtccggcaa gacaatcctg
3721 gatttcctga agtccgacgg cttcgccaac agaaacttca tgcagctgat ccacgacgac
3781 agcctgacct ttaaagagga catccagaaa gcccaggtgt ccggccaggg cgatagcctg
3841 cacgagcaca ttgccaatct ggccggcagc cccgccatta agaagggcat cctgcagaca
3901 gtgaaggtgg tggacgagct cgtgaaagtg atgggccggc acaagcccga gaacatcgtg
3961 atcgaaatgg ccagagagaa ccagaccacc cagaagggac agaagaacag ccgcgagaga
4021 atgaagcgga tcgaagaggg catcaaagag ctgggcagcc agatcctgaa agaacacccc
4081 gtggaaaaca cccagctgca gaacgagaag ctgtacctgt actacctgca gaatgggcgg
4141 gatatgtacg tggaccagga actggacatc aaccggctgt ccgactacga tgtggaccat
4201 atcgtgcctc agagctttct gaaggacgac tccatcgaca acaaggtgct gaccagaagc
4261 gacaagaacc ggggcaagag cgacaacgtg ccctccgaag aggtcgtgaa gaagatgaag
4321 aactactggc ggcagctgct gaacgccaag ctgattaccc agagaaagtt cgacaatctg
4381 accaaggccg agagaggcgg cctgagcgaa ctggataagg ccggcttcat caagagacag
4441 ctggtggaaa cccggcagat cacaaagcac gtggcacaga tcctggactc ccggatgaac
4501 actaagtacg acgagaatga caagctgatc cgggaagtga aagtgatcac cctgaagtcc
4561 aagctggtgt ccgatttccg gaaggatttc cagttttaca aagtgcgcga gatcaacaac
4621 taccaccacg cccacgacgc ctacctgaac gccgtcgtgg gaaccgccct gatcaaaaag
4681 taccctaagc tggaaagcga gttcgtgtac ggcgactaca aggtgtacga cgtgcggaag
4741 atgatcgcca agagcgagca ggaaatcggc aaggctaccg ccaagtactt cttctacagc
4801 aacatcatga actttttcaa gaccgagatt accctggcca acggcgagat ccggaagcgg
4861 cctctgatcg agacaaacgg cgaaaccggg gagatcgtgt gggataaggg ccgggatttt
4921 gccaccgtgc ggaaagtgct gagcatgccc caagtgaata tcgtgaaaaa gaccgaggtg
4981 cagacaggcg gcttcagcaa agagtctatc ctgcccaaga ggaacagcga taagctgatc
5041 gccagaaaga aggactggga ccctaagaag tacggcggct tcgacagccc caccgtggcc
5101 tattctgtgc tggtggtggc caaagtggaa aagggcaagt ccaagaaact gaagagtgtg
5161 aaagagctgc tggggatcac catcatggaa agaagcagct tcgagaagaa tcccatcgac
5221 tttctggaag ccaagggcta caaagaagtg aaaaaggacc tgatcatcaa gctgcctaag
5281 tactccctgt tcgagctgga aaacggccgg aagagaatgc tggcctctgc cggcgaactg
5341 cagaagggaa acgaactggc cctgccctcc aaatatgtga acttcctgta cctggccagc
5401 cactatgaga agctgaaggg ctcccccgag gataatgagc agaaacagct gtttgtggaa
5461 cagcacaagc actacctgga cgagatcatc gagcagatca gcgagttctc caagagagtg
5521 atcctggccg acgctaatct ggacaaagtg ctgtccgcct acaacaagca ccgggataag
5581 cccatcagag agcaggccga gaatatcatc cacctgttta ccctgaccaa tctgggagcc
5641 cctgccgcct tcaagtactt tgacaccacc atcgaccgga agaggtacac cagcaccaaa
5701 gaggtgctgg acgccaccct gatccaccag agcatcaccg gcctgtacga gacacggatc
5761 gacctgtctc agctgggagg cgacaaaagg ccggcggcca cgaaaaaggc cggccaggca
5821 aaaaagaaaa agacgcgtca gtgtactaat tatgctctct tgaaattggc tggagatgtt
5881 gagagcaacc caggtcccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
5941 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
6001 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
6061 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc
6121 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
6181 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
6241 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
6301 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
6361 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac
6421 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
6481 ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag
6541 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
6601 gacgagctgt acaagtaact cgagctgtgc cttctagttg ccagccatct gttgtttgcc
6661 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
6721 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
6781 ggcaggacag caagggggag gattgggaag agaatagcag gcatgctggg gaaagcttgg
6841 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
6901 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
6961 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
7021 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
7081 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
7141 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
7201 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
7261 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
7321 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
7381 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
7441 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
7501 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
7561 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
7621 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
7681 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
7741 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
7801 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
7861 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
7921 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
7981 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
8041 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
8101 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
8161 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
8221 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
8281 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
8341 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
8401 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
8461 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
8521 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
8581 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
8641 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
8701 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
8761 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
8821 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
8881 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
8941 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
9001 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
9061 ggccctttcg tc
//
***Note: The text map shown below is representative of the parental
vector only and does not include the gene of interest. The sequence of the gene
can be found through the accession number link on the product page.
1 tcgcgcgtttcggtgatgacggtgaaaacctctgacacatgcagctcccggagacggtca 60
AGCGCGCAAAGCCACTACTGCCACTTTTGGAGACTGTGTACGTCGAGGGCCTCTGCCAGT
61 cagcttgtctgtaagcggatgccgggagcagacaagcccgtcagggcgcgtcagcgggtg 120
GTCGAACAGACATTCGCCTACGGCCCTCGTCTGTTCGGGCAGTCCCGCGCAGTCGCCCAC
121 ttggcgggtgtcggggctggcttaactatgcggcatcagagcagattgtactgagagtgc 180
AACCGCCCACAGCCCCGACCGAATTGATACGCCGTAGTCTCGTCTAACATGACTCTCACG
NdeI lacZ_a reporter(239,398)<<<
| |
181 accatatgcggtgtgaaataccgcacagatgcgtaaggagaaaataccgcatcaggcgcc 240
TGGTATACGCCACACTTTATGGCGTGTCTACGCATTCCTCTTTTATGGCGTAGTCCGCGG
241 attcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgctat 300
TAAGCGGTAAGTCCGACGCGTTGACAACCCTTCCCGCTAGCCACGCCCGGAGAAGCGATA
301 tacgccagctggcgaaagggggatgtgctgcaaggcgattaagttgggtaacgccagggt 360
ATGCGGTCGACCGCTTTCCCCCTACACGACGTTCCGCTAATTCAACCCATTGCGGTCCCA
NruI
|
EcoRI KpnI
| | |
361 tttcccagtcacgacgttgtaaaacgacggccagtgaattcgagctcggtacctcgcgaa 420
AAAGGGTCAGTGCTGCAACATTTTGCTGCCGGTCACTTAAGCTCGAGCCATGGAGCGCTT
ORF_3 rf(5)(426,1358)<<<
|
XbaI EF1a prom(441,1618)>>>
| |
421 tgcatctagatatccctaggGGCTCCGGTGCCCGTCAGTGGGCAGAGCGCACATCGCCCA 480
ACGTAGATCTATAGGGATCCCCGAGGCCACGGGCAGTCACCCGTCTCGCGTGTAGCGGGT
481 CAGTCCCCGAGAAGTTGGGGGGAGGGGTCGGCAATTGAACCGGTGCCTAGAGAAGGTGGC 540
GTCAGGGGCTCTTCAACCCCCCTCCCCAGCCGTTAACTTGGCCACGGATCTCTTCCACCG
541 GCGGGGTAAACTGGGAAAGTGATGTCGTGTACTGGCTCCGCCTTTTTCCCGAGGGTGGGG 600
CGCCCCATTTGACCCTTTCACTACAGCACATGACCGAGGCGGAAAAAGGGCTCCCACCCC
601 GAGAACCGTATATAAGTGCAGTAGTCGCCGTGAACGTTCTTTTTCGCAACGGGTTTGCCG 660
CTCTTGGCATATATTCACGTCATCAGCGGCACTTGCAAGAAAAAGCGTTGCCCAAACGGC
661 CCAGAACACAGGTAAGTGCCGTGTGTGGTTCCCGCGGGCCTGGCCTCTTTACGGGTTATG 720
GGTCTTGTGTCCATTCACGGCACACACCAAGGGCGCCCGGACCGGAGAAATGCCCAATAC
721 GCCCTTGCGTGCCTTGAATTACTTCCACTGGCTGCAGTACGTGATTCTTGATCCCGAGCT 780
CGGGAACGCACGGAACTTAATGAAGGTGACCGACGTCATGCACTAAGAACTAGGGCTCGA
781 TCGGGTTGGAAGTGGGTGGGAGAGTTCGAGGCCTTGCGCTTAAGGAGCCCCTTCGCCTCG 840
AGCCCAACCTTCACCCACCCTCTCAAGCTCCGGAACGCGAATTCCTCGGGGAAGCGGAGC
841 TGCTTGAGTTGAGGCCTGGCCTGGGCGCTGGGGCCGCCGCGTGCGAATCTGGTGGCACCT 900
ACGAACTCAACTCCGGACCGGACCCGCGACCCCGGCGGCGCACGCTTAGACCACCGTGGA
901 TCGCGCCTGTCTCGCTGCTTTCGATAAGTCTCTAGCCATTTAAAATTTTTGATGACCTGC 960
AGCGCGGACAGAGCGACGAAAGCTATTCAGAGATCGGTAAATTTTAAAAACTACTGGACG
961 TGCGACGCTTTTTTTCTGGCAAGATAGTCTTGTAAATGCGGGCCAAGATCTGCACACTGG 1020
ACGCTGCGAAAAAAAGACCGTTCTATCAGAACATTTACGCCCGGTTCTAGACGTGTGACC
1021 TATTTCGGTTTTTGGGGCCGCGGGCGGCGACGGGGCCCGTGCGTCCCAGCGCACATGTTC 1080
ATAAAGCCAAAAACCCCGGCGCCCGCCGCTGCCCCGGGCACGCAGGGTCGCGTGTACAAG
1081 GGCGAGGCGGGGCCTGCGAGCGCGGCCACCGAGAATCGGACGGGGGTAGTCTCAAGCTGG 1140
CCGCTCCGCCCCGGACGCTCGCGCCGGTGGCTCTTAGCCTGCCCCCATCAGAGTTCGACC
1141 CCGGCCTGCTCTGGTGCCTGGCCTCGCGCCGCCGTGTATCGCCCCGCCCTGGGCGGCAAG 1200
GGCCGGACGAGACCACGGACCGGAGCGCGGCGGCACATAGCGGGGCGGGACCCGCCGTTC
1201 GCTGGCCCGGTCGGCACCAGTTGCGTGAGCGGAAAGATGGCCGCTTCCCGGCCCTGCTGC 1260
CGACCGGGCCAGCCGTGGTCAACGCACTCGCCTTTCTACCGGCGAAGGGCCGGGACGACG
1261 AGGGAGCTCAAAATGGAGGACGCGGCGCTCGGGAGAGCGGGCGGGTGAGTCACCCACACA 1320
TCCCTCGAGTTTTACCTCCTGCGCCGCGAGCCCTCTCGCCCGCCCACTCAGTGGGTGTGT
1321 AAGGAAAAGGGCCTTTCCGTCCTCAGCCGTCGCTTCATGTGACTCCACGGAGTACCGGGC 1380
TTCCTTTTCCCGGAAAGGCAGGAGTCGGCAGCGAAGTACACTGAGGTGCCTCATGGCCCG
1381 GCCGTCCAGGCACCTCGATTAGTTCTCGAGCTTTTGGAGTACGTCGTCTTTAGGTTGGGG 1440
CGGCAGGTCCGTGGAGCTAATCAAGAGCTCGAAAACCTCATGCAGCAGAAATCCAACCCC
1441 GGAGGGGTTTTATGCGATGGAGTTTCCCCACACTGAGTGGGTGGAGACTGAAGTTAGGCC 1500
CCTCCCCAAAATACGCTACCTCAAAGGGGTGTGACTCACCCACCTCTGACTTCAATCCGG
1501 AGCTTGGCACTTGATGTAATTCTCCTTGGAATTTGCCCTTTTTGAGTTTGGATCTTGGTT 1560
TCGAACCGTGAACTACATTAAGAGGAACCTTAAACGGGAAAAACTCAAACCTAGAACCAA
ORF_2 rf(4)(1606,4200)<<<
|
1561 CATTCTCAAGCCTCAGACAGTGGTTCAAAGTTTTTTTCTTCCATTTCAGGTGTCGTGAgc 1620
GTAAGAGTTCGGAGTCTGTCACCAAGTTTCAAAAAAAGAAGGTAAAGTCCACAGCACTCG
ORF_1 rf(1)(1633,6618)>>>
|
1621 tagcacctcgccaTGGCCCCAAAGAAGAAGCGGAAGGTCGGTATCCACGGAGTCCCAGCA 1680
ATCGTGGAGCGGTACCGGGGTTTCTTCTTCGCCTTCCAGCCATAGGTGCCTCAGGGTCGT
1681 GCCGACAAGAAGTACAGCATCGGCCTGGACATCGGCACCAACTCTGTGGGCTGGGCCGTG 1740
CGGCTGTTCTTCATGTCGTAGCCGGACCTGTAGCCGTGGTTGAGACACCCGACCCGGCAC
1741 ATCACCGACGAGTACAAGGTGCCCAGCAAGAAATTCAAGGTGCTGGGCAACACCGACCGG 1800
TAGTGGCTGCTCATGTTCCACGGGTCGTTCTTTAAGTTCCACGACCCGTTGTGGCTGGCC
1801 CACAGCATCAAGAAGAACCTGATCGGAGCCCTGCTGTTCGACAGCGGCGAAACAGCCGAG 1860
GTGTCGTAGTTCTTCTTGGACTAGCCTCGGGACGACAAGCTGTCGCCGCTTTGTCGGCTC
1861 GCCACCCGGCTGAAGAGAACCGCCAGAAGAAGATACACCAGACGGAAGAACCGGATCTGC 1920
CGGTGGGCCGACTTCTCTTGGCGGTCTTCTTCTATGTGGTCTGCCTTCTTGGCCTAGACG
1921 TATCTGCAAGAGATCTTCAGCAACGAGATGGCCAAGGTGGACGACAGCTTCTTCCACAGA 1980
ATAGACGTTCTCTAGAAGTCGTTGCTCTACCGGTTCCACCTGCTGTCGAAGAAGGTGTCT
1981 CTGGAAGAGTCCTTCCTGGTGGAAGAGGATAAGAAGCACGAGCGGCACCCCATCTTCGGC 2040
GACCTTCTCAGGAAGGACCACCTTCTCCTATTCTTCGTGCTCGCCGTGGGGTAGAAGCCG
2041 AACATCGTGGACGAGGTGGCCTACCACGAGAAGTACCCCACCATCTACCACCTGAGAAAG 2100
TTGTAGCACCTGCTCCACCGGATGGTGCTCTTCATGGGGTGGTAGATGGTGGACTCTTTC
2101 AAACTGGTGGACAGCACCGACAAGGCCGACCTGCGGCTGATCTATCTGGCCCTGGCCCAC 2160
TTTGACCACCTGTCGTGGCTGTTCCGGCTGGACGCCGACTAGATAGACCGGGACCGGGTG
2161 ATGATCAAGTTCCGGGGCCACTTCCTGATCGAGGGCGACCTGAACCCCGACAACAGCGAC 2220
TACTAGTTCAAGGCCCCGGTGAAGGACTAGCTCCCGCTGGACTTGGGGCTGTTGTCGCTG
2221 GTGGACAAGCTGTTCATCCAGCTGGTGCAGACCTACAACCAGCTGTTCGAGGAAAACCCC 2280
CACCTGTTCGACAAGTAGGTCGACCACGTCTGGATGTTGGTCGACAAGCTCCTTTTGGGG
2281 ATCAACGCCAGCGGCGTGGACGCCAAGGCCATCCTGTCTGCCAGACTGAGCAAGAGCAGA 2340
TAGTTGCGGTCGCCGCACCTGCGGTTCCGGTAGGACAGACGGTCTGACTCGTTCTCGTCT
2341 CGGCTGGAAAATCTGATCGCCCAGCTGCCCGGCGAGAAGAAGAATGGCCTGTTCGGcAAC 2400
GCCGACCTTTTAGACTAGCGGGTCGACGGGCCGCTCTTCTTCTTACCGGACAAGCCGTTG
2401 CTGATTGCCCTGAGCCTGGGCCTGACCCCCAACTTCAAGAGCAACTTCGACCTGGCCGAG 2460
GACTAACGGGACTCGGACCCGGACTGGGGGTTGAAGTTCTCGTTGAAGCTGGACCGGCTC
2461 GATGCCAAACTGCAGCTGAGCAAGGACACCTACGACGACGACCTGGACAACCTGCTGGCC 2520
CTACGGTTTGACGTCGACTCGTTCCTGTGGATGCTGCTGCTGGACCTGTTGGACGACCGG
2521 CAGATCGGCGACCAGTACGCCGACCTGTTTCTGGCCGCCAAGAACCTGTCCGACGCCATC 2580
GTCTAGCCGCTGGTCATGCGGCTGGACAAAGACCGGCGGTTCTTGGACAGGCTGCGGTAG
2581 CTGCTGAGCGACATCCTGAGAGTGAACACCGAGATCACCAAGGCCCCCCTGAGCGCCTCT 2640
GACGACTCGCTGTAGGACTCTCACTTGTGGCTCTAGTGGTTCCGGGGGGACTCGCGGAGA
2641 ATGATCAAGAGATACGACGAGCACCACCAGGACCTGACCCTGCTGAAAGCTCTCGTGCGG 2700
TACTAGTTCTCTATGCTGCTCGTGGTGGTCCTGGACTGGGACGACTTTCGAGAGCACGCC
2701 CAGCAGCTGCCTGAGAAGTACAAAGAGATTTTCTTCGACCAGAGCAAGAACGGCTACGCC 2760
GTCGTCGACGGACTCTTCATGTTTCTCTAAAAGAAGCTGGTCTCGTTCTTGCCGATGCGG
2761 GGCTACATTGACGGCGGAGCCAGCCAGGAAGAGTTCTACAAGTTCATCAAGCCCATCCTG 2820
CCGATGTAACTGCCGCCTCGGTCGGTCCTTCTCAAGATGTTCAAGTAGTTCGGGTAGGAC
2821 GAAAAGATGGACGGCACCGAGGAACTGCTCGTGAAGCTGAACAGAGAGGACCTGCTGCGG 2880
CTTTTCTACCTGCCGTGGCTCCTTGACGAGCACTTCGACTTGTCTCTCCTGGACGACGCC
2881 AAGCAGCGGACCTTCGACAACGGCAGCATCCCCCACCAGATCCACCTGGGAGAGCTGCAC 2940
TTCGTCGCCTGGAAGCTGTTGCCGTCGTAGGGGGTGGTCTAGGTGGACCCTCTCGACGTG
2941 GCCATTCTGCGGCGGCAGGAAGATTTTTACCCATTCCTGAAGGACAACCGGGAAAAGATC 3000
CGGTAAGACGCCGCCGTCCTTCTAAAAATGGGTAAGGACTTCCTGTTGGCCCTTTTCTAG
3001 GAGAAGATCCTGACCTTCCGCATCCCCTACTACGTGGGCCCTCTGGCCAGGGGAAACAGC 3060
CTCTTCTAGGACTGGAAGGCGTAGGGGATGATGCACCCGGGAGACCGGTCCCCTTTGTCG
3061 AGATTCGCCTGGATGACCAGAAAGAGCGAGGAAACCATCACCCCCTGGAACTTCGAGGAA 3120
TCTAAGCGGACCTACTGGTCTTTCTCGCTCCTTTGGTAGTGGGGGACCTTGAAGCTCCTT
3121 GTGGTGGACAAGGGCGCTTCCGCCCAGAGCTTCATCGAGCGGATGACCAACTTCGATAAG 3180
CACCACCTGTTCCCGCGAAGGCGGGTCTCGAAGTAGCTCGCCTACTGGTTGAAGCTATTC
3181 AACCTGCCCAACGAGAAGGTGCTGCCCAAGCACAGCCTGCTGTACGAGTACTTCACCGTG 3240
TTGGACGGGTTGCTCTTCCACGACGGGTTCGTGTCGGACGACATGCTCATGAAGTGGCAC
3241 TATAACGAGCTGACCAAAGTGAAATACGTGACCGAGGGAATGAGAAAGCCCGCCTTCCTG 3300
ATATTGCTCGACTGGTTTCACTTTATGCACTGGCTCCCTTACTCTTTCGGGCGGAAGGAC
3301 AGCGGCGAGCAGAAAAAGGCCATCGTGGACCTGCTGTTCAAGACCAACCGGAAAGTGACC 3360
TCGCCGCTCGTCTTTTTCCGGTAGCACCTGGACGACAAGTTCTGGTTGGCCTTTCACTGG
3361 GTGAAGCAGCTGAAAGAGGACTACTTCAAGAAAATCGAGTGCTTCGACTCCGTGGAAATC 3420
CACTTCGTCGACTTTCTCCTGATGAAGTTCTTTTAGCTCACGAAGCTGAGGCACCTTTAG
3421 TCCGGCGTGGAAGATCGGTTCAACGCCTCCCTGGGCACATACCACGATCTGCTGAAAATT 3480
AGGCCGCACCTTCTAGCCAAGTTGCGGAGGGACCCGTGTATGGTGCTAGACGACTTTTAA
3481 ATCAAGGACAAGGACTTCCTGGACAATGAGGAAAACGAGGACATTCTGGAAGATATCGTG 3540
TAGTTCCTGTTCCTGAAGGACCTGTTACTCCTTTTGCTCCTGTAAGACCTTCTATAGCAC
3541 CTGACCCTGACACTGTTTGAGGACAGAGAGATGATCGAGGAACGGCTGAAAACCTATGCC 3600
GACTGGGACTGTGACAAACTCCTGTCTCTCTACTAGCTCCTTGCCGACTTTTGGATACGG
3601 CACCTGTTCGACGACAAAGTGATGAAGCAGCTGAAGCGGCGGAGATACACCGGCTGGGGC 3660
GTGGACAAGCTGCTGTTTCACTACTTCGTCGACTTCGCCGCCTCTATGTGGCCGACCCCG
3661 AGGCTGAGCCGGAAGCTGATCAACGGCATCCGGGACAAGCAGTCCGGCAAGACAATCCTG 3720
TCCGACTCGGCCTTCGACTAGTTGCCGTAGGCCCTGTTCGTCAGGCCGTTCTGTTAGGAC
3721 GATTTCCTGAAGTCCGACGGCTTCGCCAACAGAAACTTCATGCAGCTGATCCACGACGAC 3780
CTAAAGGACTTCAGGCTGCCGAAGCGGTTGTCTTTGAAGTACGTCGACTAGGTGCTGCTG
3781 AGCCTGACCTTTAAAGAGGACATCCAGAAAGCCCAGGTGTCCGGCCAGGGCGATAGCCTG 3840
TCGGACTGGAAATTTCTCCTGTAGGTCTTTCGGGTCCACAGGCCGGTCCCGCTATCGGAC
3841 CACGAGCACATTGCCAATCTGGCCGGCAGCCCCGCCATTAAGAAGGGCATCCTGCAGACA 3900
GTGCTCGTGTAACGGTTAGACCGGCCGTCGGGGCGGTAATTCTTCCCGTAGGACGTCTGT
3901 GTGAAGGTGGTGGACGAGCTCGTGAAAGTGATGGGCCGGCACAAGCCCGAGAACATCGTG 3960
CACTTCCACCACCTGCTCGAGCACTTTCACTACCCGGCCGTGTTCGGGCTCTTGTAGCAC
3961 ATCGAAATGGCCAGAGAGAACCAGACCACCCAGAAGGGACAGAAGAACAGCCGCGAGAGA 4020
TAGCTTTACCGGTCTCTCTTGGTCTGGTGGGTCTTCCCTGTCTTCTTGTCGGCGCTCTCT
4021 ATGAAGCGGATCGAAGAGGGCATCAAAGAGCTGGGCAGCCAGATCCTGAAAGAACACCCC 4080
TACTTCGCCTAGCTTCTCCCGTAGTTTCTCGACCCGTCGGTCTAGGACTTTCTTGTGGGG
4081 GTGGAAAACACCCAGCTGCAGAACGAGAAGCTGTACCTGTACTACCTGCAGAATGGGCGG 4140
CACCTTTTGTGGGTCGACGTCTTGCTCTTCGACATGGACATGATGGACGTCTTACCCGCC
4141 GATATGTACGTGGACCAGGAACTGGACATCAACCGGCTGTCCGACTACGATGTGGACCAT 4200
CTATACATGCACCTGGTCCTTGACCTGTAGTTGGCCGACAGGCTGATGCTACACCTGGTA
4201 ATCGTGCCTCAGAGCTTTCTGAAGGACGACTCCATCGACAACAAGGTGCTGACCAGAAGC 4260
TAGCACGGAGTCTCGAAAGACTTCCTGCTGAGGTAGCTGTTGTTCCACGACTGGTCTTCG
4261 GACAAGAACCGGGGCAAGAGCGACAACGTGCCCTCCGAAGAGGTCGTGAAGAAGATGAAG 4320
CTGTTCTTGGCCCCGTTCTCGCTGTTGCACGGGAGGCTTCTCCAGCACTTCTTCTACTTC
4321 AACTACTGGCGGCAGCTGCTGAACGCCAAGCTGATTACCCAGAGAAAGTTCGACAATCTG 4380
TTGATGACCGCCGTCGACGACTTGCGGTTCGACTAATGGGTCTCTTTCAAGCTGTTAGAC
4381 ACCAAGGCCGAGAGAGGCGGCCTGAGCGAACTGGATAAGGCCGGCTTCATCAAGAGACAG 4440
TGGTTCCGGCTCTCTCCGCCGGACTCGCTTGACCTATTCCGGCCGAAGTAGTTCTCTGTC
4441 CTGGTGGAAACCCGGCAGATCACAAAGCACGTGGCACAGATCCTGGACTCCCGGATGAAC 4500
GACCACCTTTGGGCCGTCTAGTGTTTCGTGCACCGTGTCTAGGACCTGAGGGCCTACTTG
4501 ACTAAGTACGACGAGAATGACAAGCTGATCCGGGAAGTGAAAGTGATCACCCTGAAGTCC 4560
TGATTCATGCTGCTCTTACTGTTCGACTAGGCCCTTCACTTTCACTAGTGGGACTTCAGG
4561 AAGCTGGTGTCCGATTTCCGGAAGGATTTCCAGTTTTACAAAGTGCGCGAGATCAACAAC 4620
TTCGACCACAGGCTAAAGGCCTTCCTAAAGGTCAAAATGTTTCACGCGCTCTAGTTGTTG
4621 TACCACCACGCCCACGACGCCTACCTGAACGCCGTCGTGGGAACCGCCCTGATCAAAAAG 4680
ATGGTGGTGCGGGTGCTGCGGATGGACTTGCGGCAGCACCCTTGGCGGGACTAGTTTTTC
4681 TACCCTAAGCTGGAAAGCGAGTTCGTGTACGGCGACTACAAGGTGTACGACGTGCGGAAG 4740
ATGGGATTCGACCTTTCGCTCAAGCACATGCCGCTGATGTTCCACATGCTGCACGCCTTC
4741 ATGATCGCCAAGAGCGAGCAGGAAATCGGCAAGGCTACCGCCAAGTACTTCTTCTACAGC 4800
TACTAGCGGTTCTCGCTCGTCCTTTAGCCGTTCCGATGGCGGTTCATGAAGAAGATGTCG
4801 AACATCATGAACTTTTTCAAGACCGAGATTACCCTGGCCAACGGCGAGATCCGGAAGCGG 4860
TTGTAGTACTTGAAAAAGTTCTGGCTCTAATGGGACCGGTTGCCGCTCTAGGCCTTCGCC
4861 CCTCTGATCGAGACAAACGGCGAAACCGGGGAGATCGTGTGGGATAAGGGCCGGGATTTT 4920
GGAGACTAGCTCTGTTTGCCGCTTTGGCCCCTCTAGCACACCCTATTCCCGGCCCTAAAA
4921 GCCACCGTGCGGAAAGTGCTGAGCATGCCCCAAGTGAATATCGTGAAAAAGACCGAGGTG 4980
CGGTGGCACGCCTTTCACGACTCGTACGGGGTTCACTTATAGCACTTTTTCTGGCTCCAC
4981 CAGACAGGCGGCTTCAGCAAAGAGTCTATCCTGCCCAAGAGGAACAGCGATAAGCTGATC 5040
GTCTGTCCGCCGAAGTCGTTTCTCAGATAGGACGGGTTCTCCTTGTCGCTATTCGACTAG
5041 GCCAGAAAGAAGGACTGGGACCCTAAGAAGTACGGCGGCTTCGACAGCCCCACCGTGGCC 5100
CGGTCTTTCTTCCTGACCCTGGGATTCTTCATGCCGCCGAAGCTGTCGGGGTGGCACCGG
5101 TATTCTGTGCTGGTGGTGGCCAAAGTGGAAAAGGGCAAGTCCAAGAAACTGAAGAGTGTG 5160
ATAAGACACGACCACCACCGGTTTCACCTTTTCCCGTTCAGGTTCTTTGACTTCTCACAC
5161 AAAGAGCTGCTGGGGATCACCATCATGGAAAGAAGCAGCTTCGAGAAGAATCCCATCGAC 5220
TTTCTCGACGACCCCTAGTGGTAGTACCTTTCTTCGTCGAAGCTCTTCTTAGGGTAGCTG
5221 TTTCTGGAAGCCAAGGGCTACAAAGAAGTGAAAAAGGACCTGATCATCAAGCTGCCTAAG 5280
AAAGACCTTCGGTTCCCGATGTTTCTTCACTTTTTCCTGGACTAGTAGTTCGACGGATTC
5281 TACTCCCTGTTCGAGCTGGAAAACGGCCGGAAGAGAATGCTGGCCTCTGCCGGCGAACTG 5340
ATGAGGGACAAGCTCGACCTTTTGCCGGCCTTCTCTTACGACCGGAGACGGCCGCTTGAC
5341 CAGAAGGGAAACGAACTGGCCCTGCCCTCCAAATATGTGAACTTCCTGTACCTGGCCAGC 5400
GTCTTCCCTTTGCTTGACCGGGACGGGAGGTTTATACACTTGAAGGACATGGACCGGTCG
5401 CACTATGAGAAGCTGAAGGGCTCCCCCGAGGATAATGAGCAGAAACAGCTGTTTGTGGAA 5460
GTGATACTCTTCGACTTCCCGAGGGGGCTCCTATTACTCGTCTTTGTCGACAAACACCTT
5461 CAGCACAAGCACTACCTGGACGAGATCATCGAGCAGATCAGCGAGTTCTCCAAGAGAGTG 5520
GTCGTGTTCGTGATGGACCTGCTCTAGTAGCTCGTCTAGTCGCTCAAGAGGTTCTCTCAC
5521 ATCCTGGCCGACGCTAATCTGGACAAAGTGCTGTCCGCCTACAACAAGCACCGGGATAAG 5580
TAGGACCGGCTGCGATTAGACCTGTTTCACGACAGGCGGATGTTGTTCGTGGCCCTATTC
5581 CCCATCAGAGAGCAGGCCGAGAATATCATCCACCTGTTTACCCTGACCAATCTGGGAGCC 5640
GGGTAGTCTCTCGTCCGGCTCTTATAGTAGGTGGACAAATGGGACTGGTTAGACCCTCGG
5641 CCTGCCGCCTTCAAGTACTTTGACACCACCATCGACCGGAAGAGGTACACCAGCACCAAA 5700
GGACGGCGGAAGTTCATGAAACTGTGGTGGTAGCTGGCCTTCTCCATGTGGTCGTGGTTT
5701 GAGGTGCTGGACGCCACCCTGATCCACCAGAGCATCACCGGCCTGTACGAGACACGGATC 5760
CTCCACGACCTGCGGTGGGACTAGGTGGTCTCGTAGTGGCCGGACATGCTCTGTGCCTAG
5761 GACCTGTCTCAGCTGGGAGGCGACAAAAGGCCGGCGGCCACGAAAAAGGCCGGCCAGGCA 5820
CTGGACAGAGTCGACCCTCCGCTGTTTTCCGGCCGCCGGTGCTTTTTCCGGCCGGTCCGT
5821 AAAAAGAAAAAGACGCGTCAGTGTACTAATTATGCTCTCTTGAAATTGGCTGGAGATGTT 5880
TTTTTCTTTTTCTGCGCAGTCACATGATTAATACGAGAGAACTTTAACCGACCTCTACAA
EGFP reporter(5899,6615)>>>
|
5881 GAGAGCAACCCAGGTCccatggtgagcaagggcgaggagctgttcaccggggtggtgccc 5940
CTCTCGTTGGGTCCAGGGTACCACTCGTTCCCGCTCCTCGACAAGTGGCCCCACCACGGG
5941 atcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggc 6000
TAGGACCAGCTCGACCTGCCGCTGCATTTGCCGGTGTTCAAGTCGCACAGGCCGCTCCCG
6001 gagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctg 6060
CTCCCGCTACGGTGGATGCCGTTCGACTGGGACTTCAAGTAGACGTGGTGGCCGTTCGAC
6061 cccgtgccctggcccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgc 6120
GGGCACGGGACCGGGTGGGAGCACTGGTGGGACTGGATGCCGCACGTCACGAAGTCGGCG
6121 taccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtc 6180
ATGGGGCTGGTGTACTTCGTCGTGCTGAAGAAGTTCAGGCGGTACGGGCTTCCGATGCAG
6181 caggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaag 6240
GTCCTCGCGTGGTAGAAGAAGTTCCTGCTGCCGTTGATGTTCTGGGCGCGGCTCCACTTC
6241 ttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggac 6300
AAGCTCCCGCTGTGGGACCACTTGGCGTAGCTCGACTTCCCGTAGCTGAAGTTCCTCCTG
6301 ggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatg 6360
CCGTTGTAGGACCCCGTGTTCGACCTCATGTTGATGTTGTCGGTGTTGCAGATATAGTAC
6361 gccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggac 6420
CGGCTGTTCGTCTTCTTGCCGTAGTTCCACTTGAAGTTCTAGGCGGTGTTGTAGCTCCTG
6421 ggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtg 6480
CCGTCGCACGTCGAGCGGCTGGTGATGGTCGTCTTGTGGGGGTAGCCGCTGCCGGGGCAC
6481 ctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgag 6540
GACGACGGGCTGTTGGTGATGGACTCGTGGGTCAGGCGGGACTCGTTTCTGGGGTTGCTC
6541 aagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatg 6600
TTCGCGCTAGTGTACCAGGACGACCTCAAGCACTGGCGGCGGCCCTAGTGAGAGCCGTAC
6601 gacgagctgtacaagtaactcgagctgtgccttctagttgccagccatctgttgtttgcc 6660
CTGCTCGACATGTTCATTGAGCTCGACACGGAAGATCAACGGTCGGTAGACAACAAACGG
6661 cctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaa 6720
GGAGGGGGCACGGAAGGAACTGGGACCTTCCACGGTGAGGGTGACAGGAAAGGATTATTT
6721 atgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtgg 6780
TACTCCTTTAACGTAGCGTAACAGACTCATCCACAGTAAGATAAGACCCCCCACCCCACC
HindIII
|
6781 ggcaggacagcaagggggaggattgggaagagaatagcaggcatgctggggaaagcttgg 6840
CCGTCCTGTCGTTCCCCCTCCTAACCCTTCTCTTATCGTCCGTACGACCCCTTTCGAACC
6841 cgtaatcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacaca 6900
GCATTAGTACCAGTATCGACAAAGGACACACTTTAACAATAGGCGAGTGTTAAGGTGTGT
lac prom(6900,6929)<<<
|
6901 acatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactca 6960
TGTATGCTCGGCCTTCGTATTTCACATTTCGGACCCCACGGATTACTCACTCGATTGAGT
6961 cattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgc 7020
GTAATTAACGCAACGCGAGTGACGGGCGAAAGGTCAGCCCTTTGGACAGCACGGTCGACG
7021 attaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgctt 7080
TAATTACTTAGCCGGTTGCGCGCCCCTCTCCGCCAAACGCATAACCCGCGAGAAGGCGAA
7081 cctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcact 7140
GGAGCGAGTGACTGAGCGACGCGAGCCAGCAAGCCGACGCCGCTCGCCATAGTCGAGTGA
7141 caaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgag 7200
GTTTCCGCCATTATGCCAATAGGTGTCTTAGTCCCCTATTGCGTCCTTTCTTGTACACTC
pBR322 origin(7238,7857)<<<
|
7201 caaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccata 7260
GTTTTCCGGTCGTTTTCCGGTCCTTGGCATTTTTCCGGCGCAACGACCGCAAAAAGGTAT
7261 ggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacc 7320
CCGAGGCGGGGGGACTGCTCGTAGTGTTTTTAGCTGCGAGTTCAGTCTCCACCGCTTTGG
7321 cgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctg 7380
GCTGTCCTGATATTTCTATGGTCCGCAAAGGGGGACCTTCGAGGGAGCACGCGAGAGGAC
7381 ttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgc 7440
AAGGCTGGGACGGCGAATGGCCTATGGACAGGCGGAAAGAGGGAAGCCCTTCGCACCGCG
7441 tttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgg 7500
AAAGAGTATCGAGTGCGACATCCATAGAGTCAAGCCACATCCAGCAAGCGAGGTTCGACC
7501 gctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtc 7560
CGACACACGTGCTTGGGGGGCAAGTCGGGCTGGCGACGCGGAATAGGCCATTGATAGCAG
7561 ttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacagga 7620
AACTCAGGTTGGGCCATTCTGTGCTGAATAGCGGTGACCGTCGTCGGTGACCATTGTCCT
7621 ttagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacg 7680
AATCGTCTCGCTCCATACATCCGCCACGATGTCTCAAGAACTTCACCACCGGATTGATGC
7681 gctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaa 7740
CGATGTGATCTTCTTGTCATAAACCATAGACGCGAGACGACTTCGGTCAATGGAAGCCTT
7741 aaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttg 7800
TTTCTCAACCATCGAGAACTAGGCCGTTTGTTTGGTGGCGACCATCGCCACCAAAAAAAC
7801 tttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatctttt 7860
AAACGTTCGTCGTCTAATGCGCGTCTTTTTTTCCTAGAGTTCTTCTAGGAAACTAGAAAA
7861 ctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagat 7920
GATGCCCCAGACTGCGAGTCACCTTGCTTTTGAGTGCAATTCCCTAAAACCAGTACTCTA
7921 tatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatct 7980
ATAGTTTTTCCTAGAAGTGGATCTAGGAAAATTTAATTTTTACTTCAAAATTTAGTTAGA
ORF_4 rf(6)(8012,8872)<<<
|
amp marker(8012,8872)<<<
|
7981 aaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcaccta 8040
TTTCATATATACTCATTTGAACCAGACTGTCAATGGTTACGAATTAGTCACTCCGTGGAT
8041 tctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataa 8100
AGAGTCGCTAGACAGATAAAGCAAGTAGGTATCAACGGACTGAGGGGCAGCACATCTATT
8101 ctacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccac 8160
GATGCTATGCCCTCCCGAATGGTAGACCGGGGTCACGACGTTACTATGGCGCTCTGGGTG
8161 gctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaa 8220
CGAGTGGCCGAGGTCTAAATAGTCGTTATTTGGTCGGTCGGCCTTCCCGGCTCGCGTCTT
8221 gtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagag 8280
CACCAGGACGTTGAAATAGGCGGAGGTAGGTCAGATAATTAACAACGGCCCTTCGATCTC
8281 taagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtgg 8340
ATTCATCAAGCGGTCAATTATCAAACGCGTTGCAACAACGGTAACGATGTCCGTAGCACC
8341 tgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgag 8400
ACAGTGCGAGCAGCAAACCATACCGAAGTAAGTCGAGGCCAAGGGTTGCTAGTTCCGCTC
8401 ttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttg 8460
AATGTACTAGGGGGTACAACACGTTTTTTCGCCAATCGAGGAAGCCAGGAGGCTAGCAAC
8461 tcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctc 8520
AGTCTTCATTCAACCGGCGTCACAATAGTGAGTACCAATACCGTCGTGACGTATTAAGAG
8521 ttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcat 8580
AATGACAGTACGGTAGGCATTCTACGAAAAGACACTGACCACTCATGAGTTGGTTCAGTA
8581 tctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataata 8640
AGACTCTTATCACATACGCCGCTGGCTCAACGAGAACGGGCCGCAGTTATGCCCTATTAT
8641 ccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaa 8700
GGCGCGGTGTATCGTCTTGAAATTTTCACGAGTAGTAACCTTTTGCAAGAAGCCCCGCTT
8701 aactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcaccca 8760
TTGAGAGTTCCTAGAATGGCGACAACTCTAGGTCAAGCTACATTGGGTGAGCACGTGGGT
8761 actgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggc 8820
TGACTAGAAGTCGTAGAAAATGAAAGTGGTCGCAAAGACCCACTCGTTTTTGTCCTTCCG
8821 aaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcc 8880
TTTTACGGCGTTTTTTCCCTTATTCCCGCTGTGCCTTTACAACTTATGAGTATGAGAAGG
amp prom(8914,8942)<<<
|
8881 tttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttg 8940
AAAAAGTTATAATAACTTCGTAAATAGTCCCAATAACAGAGTACTCGCCTATGTATAAAC
8941 aatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccac 9000
TTACATAAATCTTTTTATTTGTTTATCCCCAAGGCGCGTGTAAAGGGGCTTTTCACGGTG
9001 ctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacga 9060
GACTGCAGATTCTTTGGTAATAATAGTACTGTAATTGGATATTTTTATCCGCATAGTGCT
9061 ggccctttcgtc 9072
CCGGGAAAGCAG
LOCUS dna 9072 bp
FEATURES Location/Qualifiers
misc_binding 183..188
/dbxref="REBASE:NdeI"
Reporter 239..398
/gene="lacZ_a reporter"
misc_binding 396..401
/dbxref="REBASE:EcoRI"
misc_binding 408..413
/dbxref="REBASE:KpnI"
misc_binding 414..419
/dbxref="REBASE:NruI"
misc_binding 425..430
/dbxref="REBASE:XbaI"
ORF 426..1358
/sequence="ORF_3 rf(5)"
Promoter 441..1618
/gene="EF1a prom"
ORF 1606..4200
/sequence="ORF_2 rf(4)"
ORF 1633..6618
/sequence="ORF_1 rf(1)"
Reporter 5899..6615
/gene="EGFP reporter"
misc_binding 6833..6838
/dbxref="REBASE:HindIII"
Promoter 6900..6929
/gene="lac prom"
Rep_Origin 7238..7857
/gene="pBR322 origin"
Marker 8012..8872
/gene="amp marker"
ORF 8012..8872
/sequence="ORF_4 rf(6)"
Promoter 8914..8942
/gene="amp prom"
BASE COUNT 2296 a 2462 c 2548 g 1766 t 0 others