piLenti-siRNA-GFP-Scrambled
| Vector Name | piLenti-siRNA-GFP-Scrambled |
| VectorType | Lentiviral siRNA Vector |
| Antibiotic Information | Bacterial: Kanamycin Mammalian: Puromycin |
| Sequencing Primers | 5’--TACGTCCAAGGTCGGGCAGGAAGA--3’ in the U6 promoter region |
| Additional Information | Scrambled Sequence: GGGTGAACTCACGTCAGAA |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt 61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa 121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg 181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt 241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg 301 tgttactcat agcgcgtaat actctcatga gcggatacat atttgaatgt atttagaaaa 361 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtaactataa 421 cggtcctaag gtagcgaaaa tgtagtctta tgcaatactc ttgtagtctt gcaacatggt 481 aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt 541 ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat 601 tggacgaacc actgaattgc cgcattgcag agatattgta tttaagtgcc tagctcgata 661 cataaacggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg 721 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg 781 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat 841 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct 901 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg 961 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc 1021 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga 1081 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca 1141 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa 1201 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc 1261 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag 1321 gaagagcaaa acaaaagtaa gaccaccgca cagcaagccc gctgatcttc agacctggag 1381 gaggagatat gagggacatt ggagaagtga attatataaa tataaagtag taaaaattga 1441 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc 1501 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc 1561 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca 1621 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg 1681 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct 1741 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc 1801 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga 1861 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca 1921 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg 1981 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt 2041 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata 2101 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg 2161 aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc 2221 tcgacggtat cgaaagcttg ggattcgaat ttaaaagaaa aggggggatt ggggggtaca 2281 gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa gaactacaaa 2341 aacaaattac aaaaattcaa aattttcggg tttttcgaac ctaggtacgt ccaaggtcgg 2401 gcaggaagag ggcctatttc ccatgattcc ttcatatttg catatacgat acaaggctgt 2461 tagagagata attagaatta atttgactgt aaacacaaag atattagtac aaaatacgtg 2521 acgtagaaag taataatttc ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga 2581 ctatcatatg cttaccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg 2641 gaaaggacaa aaagggtgaa ctcacgtcag aattttttgg tacccttttt ttggtgtttc 2701 gtggtctcat acagaactta taagattccc aaatccaaag acatttcacg tttatggtga 2761 tttcccagaa cacatagcga catgcaaata ttgcagggcg ccactcccct gtccctcaca 2821 gccatcttcc tgccagggcg cacgcgcgct gggtgttccc gcctagtgac actgggcccg 2881 cgattccttg gagcgggttg atgacgtcag cgttctcaat tgtagtaatc aattacgggg 2941 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg 3001 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata 3061 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc 3121 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac 3181 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg 3241 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc 3301 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc 3361 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc 3421 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct 3481 ggtttagtga accgtcaggg taccggatcc cttaagtgaa caactagtgc caccatggtg 3541 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac 3601 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag 3661 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg 3721 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac 3781 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag 3841 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac 3901 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg 3961 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc 4021 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac 4081 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg 4141 agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg 4201 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa ggaattcgga 4261 agcgggcagt gcaccaacta cgccctgctg aagctggccg gcgacgtgga gagcaacccc 4321 ggccccggat ccatggccac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac 4381 gtcccccggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac 4441 accgtcgacc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg 4501 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc 4561 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg ctcgcgcatg 4621 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg 4681 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag 4741 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgctggg 4801 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc 4861 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc 4921 aagcccggtg cctgaacgcg ttccggaaat caacctctgg attacaaaat ttgtgaaaga 4981 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg 5041 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc 5101 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc 5161 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt 5221 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt 5281 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg 5341 aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg 5401 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg 5461 ccggctctgc ggcctcttcc gcgtctcgcc ttcgccctca gacgagtcgg atctcccttt 5521 gggccgcctc cccgcctgtc cggatggaag ggctaattca ctcccaacga atacaagatc 5581 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg 5641 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag 5701 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag 5761 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg 5821 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca 5881 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt 5941 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gcatctatgt cgggtgcgga 6001 gaaagaggta atgaaatggc attatgggta ttatgggtct gcattaatga atcggccaac 6061 ggatgtcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct 6121 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca 6181 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac 6241 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt 6301 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg 6361 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc 6421 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc 6481 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc 6541 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac 6601 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt 6661 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct 6721 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc 6781 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt 6841 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg 6901 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggcc 6961 atgagattat caaaaaggat cttcacctag atccttttca cgtagaaagc cagtccgcag 7021 aaacggtgct gaccccggat gaatgtcagc tactgggcta tctggacaag ggaaaacgca 7081 agcgcaaaga gaaagcaggt agcttgcagt gggcttacat ggcgatagct agactgggcg 7141 gttttatgga cagcaagcga accggaattg ccagctgggg cgccctctgg taaggttggg 7201 aagccctgca aagtaaactg gatggctttc ttgccgccaa ggatctgatg gcgcagggga 7261 tcaagctctg atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg 7321 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag 7381 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt 7441 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aagacgaggc agcgcggcta 7501 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg 7561 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt 7621 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat 7681 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg 7741 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca 7801 gccgaactgt tcgccaggct caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc 7861 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc 7921 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat 7981 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc 8041 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgaattttg 8101 ttaaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc 8161 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag 8221 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga 8281 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc 8341 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa 8401 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt 8461 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc 8521 gtccattcgc cattcaggat cgaattaatt cttaattaac atcatcaata atatacctt
LOCUS dna 8579 bp FEATURES Location/Qualifiers Other Gene 162..310 /gene="Encap other" Other Gene 668..848 /gene="HIV-1_5_LTR other" Other Gene 959..1003 /gene="HIV-1_psi_pack other" misc_binding 1045..1050 /dbxref="REBASE:NruI" Regulatory_Seq 1510..1743 /gene="RRE reg" ORF 1553..2254 /sequence="ORF_3 rf(2)" Promoter 2393..2648 /gene="hU6 prom" misc_binding 2874..2879 /dbxref="REBASE:ApaI" Reporter 3535..4251 /gene="EGFP reporter" ORF 3535..4935 /sequence="ORF_1 rf(1)" misc_binding 4252..4257 /dbxref="REBASE:EcoRI" Marker 4339..4935 /gene="puro marker" misc_binding 4385..4390 /dbxref="REBASE:XmaI" misc_binding 4385..4390 /dbxref="REBASE:SmaI" misc_binding 4444..4449 /dbxref="REBASE:HincII" misc_binding 4665..4670 /dbxref="REBASE:StuI" Other Gene 5545..5597 /gene="delta_U3 other" Other Gene 5598..5778 /gene="HIV-1_5_LTR other" Rep_Origin 6285..6904 /gene="pBR322 origin" Promoter 7162..7211 /gene="NEOKAN prom" misc_binding 7269..7274 /dbxref="REBASE:BclI" ORF 7300..8094 /sequence="ORF_2 rf(1)" Marker 7303..8091 /gene="NTP_II marker" Rep_Origin 8199..8505 /gene="f1 origin" BASE COUNT 2162 a 2128 c 2341 g 1948 t 0 others ORIGIN 1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt 61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa 121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg 181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt 241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg 301 tgttactcat agcgcgtaat actctcatga gcggatacat atttgaatgt atttagaaaa 361 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtaactataa 421 cggtcctaag gtagcgaaaa tgtagtctta tgcaatactc ttgtagtctt gcaacatggt 481 aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt 541 ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat 601 tggacgaacc actgaattgc cgcattgcag agatattgta tttaagtgcc tagctcgata 661 cataaacggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg 721 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg 781 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat 841 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct 901 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg 961 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc 1021 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga 1081 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca 1141 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa 1201 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc 1261 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag 1321 gaagagcaaa acaaaagtaa gaccaccgca cagcaagccc gctgatcttc agacctggag 1381 gaggagatat gagggacatt ggagaagtga attatataaa tataaagtag taaaaattga 1441 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc 1501 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc 1561 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca 1621 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg 1681 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct 1741 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc 1801 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga 1861 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca 1921 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg 1981 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt 2041 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata 2101 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg 2161 aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc 2221 tcgacggtat cgaaagcttg ggattcgaat ttaaaagaaa aggggggatt ggggggtaca 2281 gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa gaactacaaa 2341 aacaaattac aaaaattcaa aattttcggg tttttcgaac ctaggtacgt ccaaggtcgg 2401 gcaggaagag ggcctatttc ccatgattcc ttcatatttg catatacgat acaaggctgt 2461 tagagagata attagaatta atttgactgt aaacacaaag atattagtac aaaatacgtg 2521 acgtagaaag taataatttc ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga 2581 ctatcatatg cttaccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg 2641 gaaaggacaa aaagggtgaa ctcacgtcag aattttttgg tacccttttt ttggtgtttc 2701 gtggtctcat acagaactta taagattccc aaatccaaag acatttcacg tttatggtga 2761 tttcccagaa cacatagcga catgcaaata ttgcagggcg ccactcccct gtccctcaca 2821 gccatcttcc tgccagggcg cacgcgcgct gggtgttccc gcctagtgac actgggcccg 2881 cgattccttg gagcgggttg atgacgtcag cgttctcaat tgtagtaatc aattacgggg 2941 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg 3001 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata 3061 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc 3121 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac 3181 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg 3241 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc 3301 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc 3361 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc 3421 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct 3481 ggtttagtga accgtcaggg taccggatcc cttaagtgaa caactagtgc caccatggtg 3541 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac 3601 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag 3661 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg 3721 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac 3781 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag 3841 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac 3901 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg 3961 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc 4021 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac 4081 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg 4141 agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg 4201 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa ggaattcgga 4261 agcgggcagt gcaccaacta cgccctgctg aagctggccg gcgacgtgga gagcaacccc 4321 ggccccggat ccatggccac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac 4381 gtcccccggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac 4441 accgtcgacc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg 4501 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc 4561 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg ctcgcgcatg 4621 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg 4681 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag 4741 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgctggg 4801 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc 4861 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc 4921 aagcccggtg cctgaacgcg ttccggaaat caacctctgg attacaaaat ttgtgaaaga 4981 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg 5041 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc 5101 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc 5161 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt 5221 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt 5281 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg 5341 aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg 5401 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg 5461 ccggctctgc ggcctcttcc gcgtctcgcc ttcgccctca gacgagtcgg atctcccttt 5521 gggccgcctc cccgcctgtc cggatggaag ggctaattca ctcccaacga atacaagatc 5581 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg 5641 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag 5701 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag 5761 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg 5821 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca 5881 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt 5941 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gcatctatgt cgggtgcgga 6001 gaaagaggta atgaaatggc attatgggta ttatgggtct gcattaatga atcggccaac 6061 ggatgtcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct 6121 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca 6181 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac 6241 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt 6301 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg 6361 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc 6421 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc 6481 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc 6541 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac 6601 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt 6661 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct 6721 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc 6781 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt 6841 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg 6901 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggcc 6961 atgagattat caaaaaggat cttcacctag atccttttca cgtagaaagc cagtccgcag 7021 aaacggtgct gaccccggat gaatgtcagc tactgggcta tctggacaag ggaaaacgca 7081 agcgcaaaga gaaagcaggt agcttgcagt gggcttacat ggcgatagct agactgggcg 7141 gttttatgga cagcaagcga accggaattg ccagctgggg cgccctctgg taaggttggg 7201 aagccctgca aagtaaactg gatggctttc ttgccgccaa ggatctgatg gcgcagggga 7261 tcaagctctg atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg 7321 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag 7381 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt 7441 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aagacgaggc agcgcggcta 7501 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg 7561 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt 7621 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat 7681 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg 7741 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca 7801 gccgaactgt tcgccaggct caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc 7861 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc 7921 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat 7981 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc 8041 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgaattttg 8101 ttaaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc 8161 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag 8221 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga 8281 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc 8341 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa 8401 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt 8461 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc 8521 gtccattcgc cattcaggat cgaattaatt cttaattaac atcatcaata atatacctt //
***Note: The text map shown below is representative of the parental
vector only and does not include the gene of interest. The sequence of the gene
can be found through the accession number link on the product page.
1 ttttggattgaagccaatatgataatgagggggtggagtttgtgacgtggcgcggggcgt 60 AAAACCTAACTTCGGTTATACTATTACTCCCCCACCTCAAACACTGCACCGCGCCCCGCA 61 gggaacggggcgggtgacgtagtagtgtggcggaagtgtgatgttgcaagtgtggcggaa 120 CCCTTGCCCCGCCCACTGCATCATCACACCGCCTTCACACTACAACGTTCACACCGCCTT Encap other(162,310)>>> | 121 cacatgtaagcgacggatgtggcaaaagtgacgtttttggtgtgcgccggtgtacacagg 180 GTGTACATTCGCTGCCTACACCGTTTTCACTGCAAAAACCACACGCGGCCACATGTGTCC 181 aagtgacaattttcgcgcggttttaggcggatgttgtagtaaatttgggcgtaaccgagt 240 TTCACTGTTAAAAGCGCGCCAAAATCCGCCTACAACATCATTTAAACCCGCATTGGCTCA 241 aagatttggccattttcgcgggaaaactgaataagaggaagtgaaatctgaataattttg 300 TTCTAAACCGGTAAAAGCGCCCTTTTGACTTATTCTCCTTCACTTTAGACTTATTAAAAC 301 tgttactcatagcgcgtaatactctcatgagcggatacatatttgaatgtatttagaaaa 360 ACAATGAGTATCGCGCATTATGAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTT 361 ataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtaactataa 420 TATTTGTTTATCCCCAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTGCATTGATATT 421 cggtcctaaggtagcgaaaatgtagtcttatgcaatactcttgtagtcttgcaacatggt 480 GCCAGGATTCCATCGCTTTTACATCAGAATACGTTATGAGAACATCAGAACGTTGTACCA 481 aacgatgagttagcaacatgccttacaaggagagaaaaagcaccgtgcatgccgattggt 540 TTGCTACTCAATCGTTGTACGGAATGTTCCTCTCTTTTTCGTGGCACGTACGGCTAACCA 541 ggaagtaaggtggtacgatcgtgccttattaggaaggcaacagacgggtctgacatggat 600 CCTTCATTCCACCATGCTAGCACGGAATAATCCTTCCGTTGTCTGCCCAGACTGTACCTA 601 tggacgaaccactgaattgccgcattgcagagatattgtatttaagtgcctagctcgata 660 ACCTGCTTGGTGACTTAACGGCGTAACGTCTCTATAACATAAATTCACGGATCGAGCTAT HIV-1_5_LTR other(668,848)>>> | 661 cataaacgggtctctctggttagaccagatctgagcctgggagctctctggctaactagg 720 GTATTTGCCCAGAGAGACCAATCTGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCC 721 gaacccactgcttaagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccg 780 CTTGGGTGACGAATTCGGAGTTATTTCGAACGGAACTCACGAAGTTCATCACACACGGGC 781 tctgttgtgtgactctggtaactagagatccctcagacccttttagtcagtgtggaaaat 840 AGACAACACACTGAGACCATTGATCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTA 841 ctctagcagtggcgcccgaacagggacttgaaagcgaaagggaaaccagaggagctctct 900 GAGATCGTCACCGCGGGCTTGTCCCTGAACTTTCGCTTTCCCTTTGGTCTCCTCGAGAGA HIV-1_psi_pack other(959,1003)>>> | 901 cgacgcaggactcggcttgctgaagcgcgcacggcaagaggcgaggggcggcgactggtg 960 GCTGCGTCCTGAGCCGAACGACTTCGCGCGTGCCGTTCTCCGCTCCCCGCCGCTGACCAC 961 agtacgccaaaaattttgactagcggaggctagaaggagagagatgggtgcgagagcgtc 1020 TCATGCGGTTTTTAAAACTGATCGCCTCCGATCTTCCTCTCTCTACCCACGCTCTCGCAG NruI | 1021 agtattaagcgggggagaattagatcgcgatgggaaaaaattcggttaaggccaggggga 1080 TCATAATTCGCCCCCTCTTAATCTAGCGCTACCCTTTTTTAAGCCAATTCCGGTCCCCCT 1081 aagaaaaaatataaattaaaacatatagtatgggcaagcagggagctagaacgattcgca 1140 TTCTTTTTTATATTTAATTTTGTATATCATACCCGTTCGTCCCTCGATCTTGCTAAGCGT 1141 gttaatcctggcctgttagaaacatcagaaggctgtagacaaatactgggacagctacaa 1200 CAATTAGGACCGGACAATCTTTGTAGTCTTCCGACATCTGTTTATGACCCTGTCGATGTT 1201 ccatcccttcagacaggatcagaagaacttagatcattatataatacagtagcaaccctc 1260 GGTAGGGAAGTCTGTCCTAGTCTTCTTGAATCTAGTAATATATTATGTCATCGTTGGGAG 1261 tattgtgtgcatcaaaggatagagataaaagacaccaaggaagctttagacaagatagag 1320 ATAACACACGTAGTTTCCTATCTCTATTTTCTGTGGTTCCTTCGAAATCTGTTCTATCTC 1321 gaagagcaaaacaaaagtaagaccaccgcacagcaagcccgctgatcttcagacctggag 1380 CTTCTCGTTTTGTTTTCATTCTGGTGGCGTGTCGTTCGGGCGACTAGAAGTCTGGACCTC 1381 gaggagatatgagggacattggagaagtgaattatataaatataaagtagtaaaaattga 1440 CTCCTCTATACTCCCTGTAACCTCTTCACTTAATATATTTATATTTCATCATTTTTAACT 1441 accattaggagtagcacccaccaaggcaaagagaagagtggtgcagagagaaaaaagagc 1500 TGGTAATCCTCATCGTGGGTGGTTCCGTTTCTCTTCTCACCACGTCTCTCTTTTTTCTCG RRE reg(1510,1743)>>> ORF_3 rf(2)(1553,2254)>>> | | 1501 agtgggaataggagctttgttccttgggttcttgggagcagcaggaagcactatgggcgc 1560 TCACCCTTATCCTCGAAACAAGGAACCCAAGAACCCTCGTCGTCCTTCGTGATACCCGCG 1561 agcgtcaatgacgctgacggtacaggccagacaattattgtctggtatagtgcagcagca 1620 TCGCAGTTACTGCGACTGCCATGTCCGGTCTGTTAATAACAGACCATATCACGTCGTCGT 1621 gaacaatttgctgagggctattgaggcgcaacagcatctgttgcaactcacagtctgggg 1680 CTTGTTAAACGACTCCCGATAACTCCGCGTTGTCGTAGACAACGTTGAGTGTCAGACCCC 1681 catcaagcagctccaggcaagaatcctggctgtggaaagatacctaaaggatcaacagct 1740 GTAGTTCGTCGAGGTCCGTTCTTAGGACCGACACCTTTCTATGGATTTCCTAGTTGTCGA 1741 cctggggatttggggttgctctggaaaactcatttgcaccactgctgtgccttggaatgc 1800 GGACCCCTAAACCCCAACGAGACCTTTTGAGTAAACGTGGTGACGACACGGAACCTTACG 1801 tagttggagtaataaatctctggaacagatttggaatcacacgacctggatggagtggga 1860 ATCAACCTCATTATTTAGAGACCTTGTCTAAACCTTAGTGTGCTGGACCTACCTCACCCT 1861 cagagaaattaacaattacacaagcttaatacactccttaattgaagaatcgcaaaacca 1920 GTCTCTTTAATTGTTAATGTGTTCGAATTATGTGAGGAATTAACTTCTTAGCGTTTTGGT 1921 gcaagaaaagaatgaacaagaattattggaattagataaatgggcaagtttgtggaattg 1980 CGTTCTTTTCTTACTTGTTCTTAATAACCTTAATCTATTTACCCGTTCAAACACCTTAAC 1981 gtttaacataacaaattggctgtggtatataaaattattcataatgatagtaggaggctt 2040 CAAATTGTATTGTTTAACCGACACCATATATTTTAATAAGTATTACTATCATCCTCCGAA 2041 ggtaggtttaagaatagtttttgctgtactttctatagtgaatagagttaggcagggata 2100 CCATCCAAATTCTTATCAAAAACGACATGAAAGATATCACTTATCTCAATCCGTCCCTAT 2101 ttcaccattatcgtttcagacccacctcccaaccccgaggggacccgacaggcccgaagg 2160 AAGTGGTAATAGCAAAGTCTGGGTGGAGGGTTGGGGCTCCCCTGGGCTGTCCGGGCTTCC 2161 aatagaagaagaaggtggagagagagacagagacagatccattcgattagtgaacggatc 2220 TTATCTTCTTCTTCCACCTCTCTCTCTGTCTCTGTCTAGGTAAGCTAATCACTTGCCTAG 2221 tcgacggtatcgaaagcttgggattcgaatttaaaagaaaaggggggattggggggtaca 2280 AGCTGCCATAGCTTTCGAACCCTAAGCTTAAATTTTCTTTTCCCCCCTAACCCCCCATGT 2281 gtgcaggggaaagaatagtagacataatagcaacagacatacaaactaaagaactacaaa 2340 CACGTCCCCTTTCTTATCATCTGTATTATCGTTGTCTGTATGTTTGATTTCTTGATGTTT hU6 prom(2393,2648)>>> | 2341 aacaaattacaaaaattcaaaattttcgggtttttcgaacctaggtacgtccaaggtcgg 2400 TTGTTTAATGTTTTTAAGTTTTAAAAGCCCAAAAAGCTTGGATCCATGCAGGTTCCAGCC 2401 gcaggaagagggcctatttcccatgattccttcatatttgcatatacgatacaaggctgt 2460 CGTCCTTCTCCCGGATAAAGGGTACTAAGGAAGTATAAACGTATATGCTATGTTCCGACA 2461 tagagagataattagaattaatttgactgtaaacacaaagatattagtacaaaatacgtg 2520 ATCTCTCTATTAATCTTAATTAAACTGACATTTGTGTTTCTATAATCATGTTTTATGCAC 2521 acgtagaaagtaataatttcttgggtagtttgcagttttaaaattatgttttaaaatgga 2580 TGCATCTTTCATTATTAAAGAACCCATCAAACGTCAAAATTTTAATACAAAATTTTACCT 2581 ctatcatatgcttaccgtaacttgaaagtatttcgatttcttggctttatatatcttgtg 2640 GATAGTATACGAATGGCATTGAACTTTCATAAAGCTAAAGAACCGAAATATATAGAACAC 2641 gaaaggacaaaaaGGGTGAACTCACGTCAGAAttttttggtaccctttttttggtgtttc 2700 CTTTCCTGTTTTTCCCACTTGAGTGCAGTCTTAAAAAACCATGGGAAAAAAACCACAAAG 2701 gtggtctcatacagaacttataagattcccaaatccaaagacatttcacgtttatggtga 2760 CACCAGAGTATGTCTTGAATATTCTAAGGGTTTAGGTTTCTGTAAAGTGCAAATACCACT 2761 tttcccagaacacatagcgacatgcaaatattgcagggcgccactcccctgtccctcaca 2820 AAAGGGTCTTGTGTATCGCTGTACGTTTATAACGTCCCGCGGTGAGGGGACAGGGAGTGT ApaI | 2821 gccatcttcctgccagggcgcacgcgcgctgggtgttcccgcctagtgacactgggcccg 2880 CGGTAGAAGGACGGTCCCGCGTGCGCGCGACCCACAAGGGCGGATCACTGTGACCCGGGC 2881 cgattccttggagcgggttgatgacgtcagcgttctcaattgtagtaatcaattacgggg 2940 GCTAAGGAACCTCGCCCAACTACTGCAGTCGCAAGAGTTAACATCATTAGTTAATGCCCC 2941 tcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccg 3000 AGTAATCAAGTATCGGGTATATACCTCAAGGCGCAATGTATTGAATGCCATTTACCGGGC 3001 cctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccata 3060 GGACCGACTGGCGGGTTGCTGGGGGCGGGTAACTGCAGTTATTACTGCATACAAGGGTAT 3061 gtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcc 3120 CATTGCGGTTATCCCTGAAAGGTAACTGCAGTTACCCACCTCATAAATGCCATTTGACGG 3121 cacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgac 3180 GTGAACCGTCATGTAGTTCACATAGTATACGGTTCATGCGGGGGATAACTGCAGTTACTG 3181 ggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttgg 3240 CCATTTACCGGGCGGACCGTAATACGGGTCATGTACTGGAATACCCTGAAAGGATGAACC 3241 cagtacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatc 3300 GTCATGTAGATGCATAATCAGTAGCGATAATGGTACCACTACGCCAAAACCGTCATGTAG 3301 aatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtc 3360 TTACCCGCACCTATCGCCAAACTGAGTGCCCCTAAAGGTTCAGAGGTGGGGTAACTGCAG 3361 aatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactcc 3420 TTACCCTCAAACAAAACCGTGGTTTTAGTTGCCCTGAAAGGTTTTACAGCATTGTTGAGG 3421 gccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagct 3480 CGGGGTAACTGCGTTTACCCGCCATCCGCACATGCCACCCTCCAGATATATTCGTCTCGA EGFP reporter(3535,4251)>>> | ORF_1 rf(1)(3535,4935)>>> | 3481 ggtttagtgaaccgtcagggtaccggatcccttaagtgaacaactagtgccaccatggtg 3540 CCAAATCACTTGGCAGTCCCATGGCCTAGGGAATTCACTTGTTGATCACGGTGGTACCAC 3541 agcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgac 3600 TCGTTCCCGCTCCTCGACAAGTGGCCCCACCACGGGTAGGACCAGCTCGACCTGCCGCTG 3601 gtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaag 3660 CATTTGCCGGTGTTCAAGTCGCACAGGCCGCTCCCGCTCCCGCTACGGTGGATGCCGTTC 3661 ctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtg 3720 GACTGGGACTTCAAGTAGACGTGGTGGCCGTTCGACGGGCACGGGACCGGGTGGGAGCAC 3721 accaccctgacctacggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcac 3780 TGGTGGGACTGGATGCCGCACGTCACGAAGTCGGCGATGGGGCTGGTGTACTTCGTCGTG 3781 gacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaag 3840 CTGAAGAAGTTCAGGCGGTACGGGCTTCCGATGCAGGTCCTCGCGTGGTAGAAGAAGTTC 3841 gacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaac 3900 CTGCTGCCGTTGATGTTCTGGGCGCGGCTCCACTTCAAGCTCCCGCTGTGGGACCACTTG 3901 cgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctg 3960 GCGTAGCTCGACTTCCCGTAGCTGAAGTTCCTCCTGCCGTTGTAGGACCCCGTGTTCGAC 3961 gagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatc 4020 CTCATGTTGATGTTGTCGGTGTTGCAGATATAGTACCGGCTGTTCGTCTTCTTGCCGTAG 4021 aaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccac 4080 TTCCACTTGAAGTTCTAGGCGGTGTTGTAGCTCCTGCCGTCGCACGTCGAGCGGCTGGTG 4081 taccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctg 4140 ATGGTCGTCTTGTGGGGGTAGCCGCTGCCGGGGCACGACGACGGGCTGTTGGTGATGGAC 4141 agcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctg 4200 TCGTGGGTCAGGCGGGACTCGTTTCTGGGGTTGCTCTTCGCGCTAGTGTACCAGGACGAC EcoRI | 4201 gagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaaggaattcgga 4260 CTCAAGCACTGGCGGCGGCCCTAGTGAGAGCCGTACCTGCTCGACATGTTCCTTAAGCCT 4261 agcgggcagtgcaccaactacgccctgctgaagctggccggcgacgtggagagcaacccc 4320 TCGCCCGTCACGTGGTTGATGCGGGACGACTTCGACCGGCCGCTGCACCTCTCGTTGGGG puro marker(4339,4935)>>> | 4321 ggccccggatccatggccaccgagtacaagcccacggtgcgcctcgccacccgcgacgac 4380 CCGGGGCCTAGGTACCGGTGGCTCATGTTCGGGTGCCACGCGGAGCGGTGGGCGCTGCTG SmaI | XmaI | | 4381 gtcccccgggccgtacgcaccctcgccgccgcgttcgccgactaccccgccacgcgccac 4440 CAGGGGGCCCGGCATGCGTGGGAGCGGCGGCGCAAGCGGCTGATGGGGCGGTGCGCGGTG HincII | 4441 accgtcgacccggaccgccacatcgagcgggtcaccgagctgcaagaactcttcctcacg 4500 TGGCAGCTGGGCCTGGCGGTGTAGCTCGCCCAGTGGCTCGACGTTCTTGAGAAGGAGTGC 4501 cgcgtcgggctcgacatcggcaaggtgtgggtcgcggacgacggcgccgcggtggcggtc 4560 GCGCAGCCCGAGCTGTAGCCGTTCCACACCCAGCGCCTGCTGCCGCGGCGCCACCGCCAG 4561 tggaccacgccggagagcgtcgaagcgggggcggtgttcgccgagatcggctcgcgcatg 4620 ACCTGGTGCGGCCTCTCGCAGCTTCGCCCCCGCCACAAGCGGCTCTAGCCGAGCGCGTAC StuI | 4621 gccgagttgagcggttcccggctggccgcgcagcaacagatggaaggcctcctggcgccg 4680 CGGCTCAACTCGCCAAGGGCCGACCGGCGCGTCGTTGTCTACCTTCCGGAGGACCGCGGC 4681 caccggcccaaggagcccgcgtggttcctggccaccgtcggcgtctcgcccgaccaccag 4740 GTGGCCGGGTTCCTCGGGCGCACCAAGGACCGGTGGCAGCCGCAGAGCGGGCTGGTGGTC 4741 ggcaagggtctgggcagcgccgtcgtgctccccggagtggaggcggccgagcgcgctggg 4800 CCGTTCCCAGACCCGTCGCGGCAGCACGAGGGGCCTCACCTCCGCCGGCTCGCGCGACCC 4801 gtgcccgccttcctggagacctccgcgccccgcaacctccccttctacgagcggctcggc 4860 CACGGGCGGAAGGACCTCTGGAGGCGCGGGGCGTTGGAGGGGAAGATGCTCGCCGAGCCG 4861 ttcaccgtcaccgccgacgtcgaggtgcccgaaggaccgcgcacctggtgcatgacccgc 4920 AAGTGGCAGTGGCGGCTGCAGCTCCACGGGCTTCCTGGCGCGTGGACCACGTACTGGGCG 4921 aagcccggtgcctgaacgcgttccggaaatcaacctctggattacaaaatttgtgaaaga 4980 TTCGGGCCACGGACTTGCGCAAGGCCTTTAGTTGGAGACCTAATGTTTTAAACACTTTCT 4981 ttgactggtattcttaactatgttgctccttttacgctatgtggatacgctgctttaatg 5040 AACTGACCATAAGAATTGATACAACGAGGAAAATGCGATACACCTATGCGACGAAATTAC 5041 cctttgtatcatgctattgcttcccgtatggctttcattttctcctccttgtataaatcc 5100 GGAAACATAGTACGATAACGAAGGGCATACCGAAAGTAAAAGAGGAGGAACATATTTAGG 5101 tggttgctgtctctttatgaggagttgtggcccgttgtcaggcaacgtggcgtggtgtgc 5160 ACCAACGACAGAGAAATACTCCTCAACACCGGGCAACAGTCCGTTGCACCGCACCACACG 5161 actgtgtttgctgacgcaacccccactggttggggcattgccaccacctgtcagctcctt 5220 TGACACAAACGACTGCGTTGGGGGTGACCAACCCCGTAACGGTGGTGGACAGTCGAGGAA 5221 tccgggactttcgctttccccctccctattgccacggcggaactcatcgccgcctgcctt 5280 AGGCCCTGAAAGCGAAAGGGGGAGGGATAACGGTGCCGCCTTGAGTAGCGGCGGACGGAA 5281 gcccgctgctggacaggggctcggctgttgggcactgacaattccgtggtgttgtcgggg 5340 CGGGCGACGACCTGTCCCCGAGCCGACAACCCGTGACTGTTAAGGCACCACAACAGCCCC 5341 aagctgacgtcctttccatggctgctcgcctgtgttgccacctggattctgcgcgggacg 5400 TTCGACTGCAGGAAAGGTACCGACGAGCGGACACAACGGTGGACCTAAGACGCGCCCTGC 5401 tccttctgctacgtcccttcggccctcaatccagcggaccttccttcccgcggcctgctg 5460 AGGAAGACGATGCAGGGAAGCCGGGAGTTAGGTCGCCTGGAAGGAAGGGCGCCGGACGAC 5461 ccggctctgcggcctcttccgcgtctcgccttcgccctcagacgagtcggatctcccttt 5520 GGCCGAGACGCCGGAGAAGGCGCAGAGCGGAAGCGGGAGTCTGCTCAGCCTAGAGGGAAA delta_U3 other(5545,5597)>>> | 5521 gggccgcctccccgcctgtccggatggaagggctaattcactcccaacgaatacaagatc 5580 CCCGGCGGAGGGGCGGACAGGCCTACCTTCCCGATTAAGTGAGGGTTGCTTATGTTCTAG HIV-1_5_LTR other(5598,5778)>>> | 5581 tgctttttgcttgtactgggtctctctggttagaccagatctgagcctgggagctctctg 5640 ACGAAAAACGAACATGACCCAGAGAGACCAATCTGGTCTAGACTCGGACCCTCGAGAGAC 5641 gctaactagggaacccactgcttaagcctcaataaagcttgccttgagtgcttcaagtag 5700 CGATTGATCCCTTGGGTGACGAATTCGGAGTTATTTCGAACGGAACTCACGAAGTTCATC 5701 tgtgtgcccgtctgttgtgtgactctggtaactagagatccctcagacccttttagtcag 5760 ACACACGGGCAGACAACACACTGAGACCATTGATCTCTAGGGAGTCTGGGAAAATCAGTC 5761 tgtggaaaatctctagcagtagtagttcatgtcatcttattattcagtatttataacttg 5820 ACACCTTTTAGAGATCGTCATCATCAAGTACAGTAGAATAATAAGTCATAAATATTGAAC 5821 caaagaaatgaatatcagagagtgagaggaacttgtttattgcagcttataatggttaca 5880 GTTTCTTTACTTATAGTCTCTCACTCTCCTTGAACAAATAACGTCGAATATTACCAATGT 5881 aataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagtt 5940 TTATTTCGTTATCGTAGTGTTTAAAGTGTTTATTTCGTAAAAAAAGTGACGTAAGATCAA 5941 gtggtttgtccaaactcatcaatgtatcttatcatgtctggcatctatgtcgggtgcgga 6000 CACCAAACAGGTTTGAGTAGTTACATAGAATAGTACAGACCGTAGATACAGCCCACGCCT 6001 gaaagaggtaatgaaatggcattatgggtattatgggtctgcattaatgaatcggccaac 6060 CTTTCTCCATTACTTTACCGTAATACCCATAATACCCAGACGTAATTACTTAGCCGGTTG 6061 ggatgtcggtgtgaaataccgcacagatgcgtaaggagaaaataccgcatcaggcgctct 6120 CCTACAGCCACACTTTATGGCGTGTCTACGCATTCCTCTTTTATGGCGTAGTCCGCGAGA 6121 tccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatca 6180 AGGCGAAGGAGCGAGTGACTGAGCGACGCGAGCCAGCAAGCCGACGCCGCTCGCCATAGT 6181 gctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaac 6240 CGAGTGAGTTTCCGCCATTATGCCAATAGGTGTCTTAGTCCCCTATTGCGTCCTTTCTTG pBR322 origin(6285,6904)<<< | 6241 atgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgttt 6300 TACACTCGTTTTCCGGTCGTTTTCCGGTCCTTGGCATTTTTCCGGCGCAACGACCGCAAA 6301 ttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtgg 6360 AAGGTATCCGAGGCGGGGGGACTGCTCGTAGTGTTTTTAGCTGCGAGTTCAGTCTCCACC 6361 cgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgc 6420 GCTTTGGGCTGTCCTGATATTTCTATGGTCCGCAAAGGGGGACCTTCGAGGGAGCACGCG 6421 tctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagc 6480 AGAGGACAAGGCTGGGACGGCGAATGGCCTATGGACAGGCGGAAAGAGGGAAGCCCTTCG 6481 gtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctcc 6540 CACCGCGAAAGAGTATCGAGTGCGACATCCATAGAGTCAAGCCACATCCAGCAAGCGAGG 6541 aagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaac 6600 TTCGACCCGACACACGTGCTTGGGGGGCAAGTCGGGCTGGCGACGCGGAATAGGCCATTG 6601 tatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggt 6660 ATAGCAGAACTCAGGTTGGGCCATTCTGTGCTGAATAGCGGTGACCGTCGTCGGTGACCA 6661 aacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcct 6720 TTGTCCTAATCGTCTCGCTCCATACATCCGCCACGATGTCTCAAGAACTTCACCACCGGA 6721 aactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttacc 6780 TTGATGCCGATGTGATCTTCCTGTCATAAACCATAGACGCGAGACGACTTCGGTCAATGG 6781 ttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggt 6840 AAGCCTTTTTCTCAACCATCGAGAACTAGGCCGTTTGTTTGGTGGCGACCATCGCCACCA 6841 ttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttg 6900 AAAAAACAAACGTTCGTCGTCTAATGCGCGTCTTTTTTTCCTAGAGTTCTTCTAGGAAAC 6901 atcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggcc 6960 TAGAAAAGATGCCCCAGACTGCGAGTCACCTTGCTTTTGAGTGCAATTCCCTAAAACCGG 6961 atgagattatcaaaaaggatcttcacctagatccttttcacgtagaaagccagtccgcag 7020 TACTCTAATAGTTTTTCCTAGAAGTGGATCTAGGAAAAGTGCATCTTTCGGTCAGGCGTC 7021 aaacggtgctgaccccggatgaatgtcagctactgggctatctggacaagggaaaacgca 7080 TTTGCCACGACTGGGGCCTACTTACAGTCGATGACCCGATAGACCTGTTCCCTTTTGCGT 7081 agcgcaaagagaaagcaggtagcttgcagtgggcttacatggcgatagctagactgggcg 7140 TCGCGTTTCTCTTTCGTCCATCGAACGTCACCCGAATGTACCGCTATCGATCTGACCCGC NEOKAN prom(7162,7211)>>> | 7141 gttttatggacagcaagcgaaccggaattgccagctggggcgccctctggtaaggttggg 7200 CAAAATACCTGTCGTTCGCTTGGCCTTAACGGTCGACCCCGCGGGAGACCATTCCAACCC 7201 aagccctgcaaagtaaactggatggctttcttgccgccaaggatctgatggcgcagggga 7260 TTCGGGACGTTTCATTTGACCTACCGAAAGAACGGCGGTTCCTAGACTACCGCGTCCCCT NTP_II marker(7303,8091)>>> | BclI ORF_2 rf(1)(7300,8094)>>> | | | 7261 tcaagctctgatcaagagacaggatgaggatcgtttcgcatgattgaacaagatggattg 7320 AGTTCGAGACTAGTTCTCTGTCCTACTCCTAGCAAAGCGTACTAACTTGTTCTACCTAAC 7321 cacgcaggttctccggccgcttgggtggagaggctattcggctatgactgggcacaacag 7380 GTGCGTCCAAGAGGCCGGCGAACCCACCTCTCCGATAAGCCGATACTGACCCGTGTTGTC 7381 acaatcggctgctctgatgccgccgtgttccggctgtcagcgcaggggcgcccggttctt 7440 TGTTAGCCGACGAGACTACGGCGGCACAAGGCCGACAGTCGCGTCCCCGCGGGCCAAGAA 7441 tttgtcaagaccgacctgtccggtgccctgaatgaactgcaagacgaggcagcgcggcta 7500 AAACAGTTCTGGCTGGACAGGCCACGGGACTTACTTGACGTTCTGCTCCGTCGCGCCGAT 7501 tcgtggctggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactgaagcg 7560 AGCACCGACCGGTGCTGCCCGCAAGGAACGCGTCGACACGAGCTGCAACAGTGACTTCGC 7561 ggaagggactggctgctattgggcgaagtgccggggcaggatctcctgtcatctcacctt 7620 CCTTCCCTGACCGACGATAACCCGCTTCACGGCCCCGTCCTAGAGGACAGTAGAGTGGAA 7621 gctcctgccgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttgat 7680 CGAGGACGGCTCTTTCATAGGTAGTACCGACTACGTTACGCCGCCGACGTATGCGAACTA 7681 ccggctacctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcgg 7740 GGCCGATGGACGGGTAAGCTGGTGGTTCGCTTTGTAGCGTAGCTCGCTCGTGCATGAGCC 7741 atggaagccggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgcca 7800 TACCTTCGGCCAGAACAGCTAGTCCTACTAGACCTGCTTCTCGTAGTCCCCGAGCGCGGT 7801 gccgaactgttcgccaggctcaaggcgagcatgcccgacggcgaggatctcgtcgtgacc 7860 CGGCTTGACAAGCGGTCCGAGTTCCGCTCGTACGGGCTGCCGCTCCTAGAGCAGCACTGG 7861 catggcgatgcctgcttgccgaatatcatggtggaaaatggccgcttttctggattcatc 7920 GTACCGCTACGGACGAACGGCTTATAGTACCACCTTTTACCGGCGAAAAGACCTAAGTAG 7921 gactgtggccggctgggtgtggcggaccgctatcaggacatagcgttggctacccgtgat 7980 CTGACACCGGCCGACCCACACCGCCTGGCGATAGTCCTGTATCGCAACCGATGGGCACTA 7981 attgctgaagagcttggcggcgaatgggctgaccgcttcctcgtgctttacggtatcgcc 8040 TAACGACTTCTCGAACCGCCGCTTACCCGACTGGCGAAGGAGCACGAAATGCCATAGCGG 8041 gctcccgattcgcagcgcatcgccttctatcgccttcttgacgagttcttctgaattttg 8100 CGAGGGCTAAGCGTCGCGTAGCGGAAGATAGCGGAAGAACTGCTCAAGAAGACTTAAAAC 8101 ttaaaatttttgttaaatcagctcattttttaaccaataggccgaaatcggcaaaatccc 8160 AATTTTAAAAACAATTTAGTCGAGTAAAAAATTGGTTATCCGGCTTTAGCCGTTTTAGGG f1 origin(8199,8505)<<< | 8161 ttataaatcaaaagaatagaccgagatagggttgagtgttgttccagtttggaacaagag 8220 AATATTTAGTTTTCTTATCTGGCTCTATCCCAACTCACAACAAGGTCAAACCTTGTTCTC 8221 tccactattaaagaacgtggactccaacgtcaaagggcgaaaaaccgtctatcagggcga 8280 AGGTGATAATTTCTTGCACCTGAGGTTGCAGTTTCCCGCTTTTTGGCAGATAGTCCCGCT 8281 tggcccactacgtgaaccatcaccctaatcaagttttttggggtcgaggtgccgtaaagc 8340 ACCGGGTGATGCACTTGGTAGTGGGATTAGTTCAAAAAACCCCAGCTCCACGGCATTTCG 8341 actaaatcggaaccctaaagggagcccccgatttagagcttgacggggaaagccggcgaa 8400 TGATTTAGCCTTGGGATTTCCCTCGGGGGCTAAATCTCGAACTGCCCCTTTCGGCCGCTT 8401 cgtggcgagaaaggaagggaagaaagcgaaaggagcgggcgctagggcgctggcaagtgt 8460 GCACCGCTCTTTCCTTCCCTTCTTTCGCTTTCCTCGCCCGCGATCCCGCGACCGTTCACA 8461 agcggtcacgctgcgcgtaaccaccacacccgccgcgcttaatgcgccgctacagggcgc 8520 TCGCCAGTGCGACGCGCATTGGTGGTGTGGGCGGCGCGAATTACGCGGCGATGTCCCGCG 8521 gtccattcgccattcaggatcgaattaattcttaattaacatcatcaataatatacctt 8579 CAGGTAAGCGGTAAGTCCTAGCTTAATTAAGAATTAATTGTAGTAGTTATTATATGGAA
LOCUS dna 8579 bp FEATURES Location/Qualifiers Other Gene 162..310 /gene="Encap other" Other Gene 668..848 /gene="HIV-1_5_LTR other" Other Gene 959..1003 /gene="HIV-1_psi_pack other" misc_binding 1045..1050 /dbxref="REBASE:NruI" Regulatory_Seq 1510..1743 /gene="RRE reg" ORF 1553..2254 /sequence="ORF_3 rf(2)" Promoter 2393..2648 /gene="hU6 prom" misc_binding 2874..2879 /dbxref="REBASE:ApaI" Reporter 3535..4251 /gene="EGFP reporter" ORF 3535..4935 /sequence="ORF_1 rf(1)" misc_binding 4252..4257 /dbxref="REBASE:EcoRI" Marker 4339..4935 /gene="puro marker" misc_binding 4385..4390 /dbxref="REBASE:XmaI" misc_binding 4385..4390 /dbxref="REBASE:SmaI" misc_binding 4444..4449 /dbxref="REBASE:HincII" misc_binding 4665..4670 /dbxref="REBASE:StuI" Other Gene 5545..5597 /gene="delta_U3 other" Other Gene 5598..5778 /gene="HIV-1_5_LTR other" Rep_Origin 6285..6904 /gene="pBR322 origin" Promoter 7162..7211 /gene="NEOKAN prom" misc_binding 7269..7274 /dbxref="REBASE:BclI" ORF 7300..8094 /sequence="ORF_2 rf(1)" Marker 7303..8091 /gene="NTP_II marker" Rep_Origin 8199..8505 /gene="f1 origin" BASE COUNT 2162 a 2128 c 2341 g 1948 t 0 others