piLenti-siRNA-GFP
| Vector Name | piLenti-siRNA-GFP |
| VectorType | Lentiviral siRNA Vector |
| Antibiotic Information | Bacterial: Kanamycin Mammalian: Puromycin |
| Sequencing Primers | 5’--TACGTCCAAGGTCGGGCAGGAAGA--3’ in the U6 promoter region |
| Additional Information | No Additional Information |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt 61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa 121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg 181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt 241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg 301 tgttactcat agcgcgtaat actctcatga gcggatacat atttgaatgt atttagaaaa 361 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtaactataa 421 cggtcctaag gtagcgaaaa tgtagtctta tgcaatactc ttgtagtctt gcaacatggt 481 aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt 541 ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat 601 tggacgaacc actgaattgc cgcattgcag agatattgta tttaagtgcc tagctcgata 661 cataaacggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg 721 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg 781 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat 841 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct 901 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg 961 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc 1021 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga 1081 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca 1141 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa 1201 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc 1261 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag 1321 gaagagcaaa acaaaagtaa gaccaccgca cagcaagccc gctgatcttc agacctggag 1381 gaggagatat gagggacatt ggagaagtga attatataaa tataaagtag taaaaattga 1441 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc 1501 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc 1561 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca 1621 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg 1681 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct 1741 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc 1801 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga 1861 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca 1921 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg 1981 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt 2041 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata 2101 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg 2161 aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc 2221 tcgacggtat cgaaagcttg ggattcgaat ttaaaagaaa aggggggatt ggggggtaca 2281 gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa gaactacaaa 2341 aacaaattac aaaaattcaa aattttcggg tttttcgaac ctaggtacgt ccaaggtcgg 2401 gcaggaagag ggcctatttc ccatgattcc ttcatatttg catatacgat acaaggctgt 2461 tagagagata attagaatta atttgactgt aaacacaaag atattagtac aaaatacgtg 2521 acgtagaaag taataatttc ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga 2581 ctatcatatg cttaccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg 2641 gaaaggacaa aaatagtctt ctctagagat atcatttaaa tgtcgacgaa ttcctcgaga 2701 gaagacagct ttttttggtg tttcgtggtc tcatacagaa cttataagat tcccaaatcc 2761 aaagacattt cacgtttatg gtgatttccc agaacacata gcgacatgca aatattgcag 2821 ggcgccactc ccctgtccct cacagccatc ttcctgccag ggcgcacgcg cgctgggtgt 2881 tcccgcctag tgacactggg cccgcgattc cttggagcgg gttgatgacg tcagcgttct 2941 caattgtagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt 3001 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg 3061 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg 3121 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt 3181 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg 3241 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg 3301 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt 3361 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac 3421 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg 3481 tgggaggtct atataagcag agctggttta gtgaaccgtc agggtaccgg atcccttaag 3541 tgaacaacta gtgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc 3601 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc 3661 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg 3721 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc 3781 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc 3841 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag 3901 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac 3961 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg 4021 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac 4081 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg 4141 ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag 4201 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg 4261 gacgagctgt acaaggaatt cggaagcggg cagtgcacca actacgccct gctgaagctg 4321 gccggcgacg tggagagcaa ccccggcccc ggatccatgg ccaccgagta caagcccacg 4381 gtgcgcctcg ccacccgcga cgacgtcccc cgggccgtac gcaccctcgc cgccgcgttc 4441 gccgactacc ccgccacgcg ccacaccgtc gacccggacc gccacatcga gcgggtcacc 4501 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg 4561 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg 4621 ttcgccgaga tcggctcgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa 4681 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc 4741 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga 4801 gtggaggcgg ccgagcgcgc tggggtgccc gccttcctgg agacctccgc gccccgcaac 4861 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga 4921 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgaa cgcgttccgg aaatcaacct 4981 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg 5041 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc 5101 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt 5161 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc 5221 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg 5281 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact 5341 gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt 5401 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg 5461 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct cgccttcgcc 5521 ctcagacgag tcggatctcc ctttgggccg cctccccgcc tgtccggatg gaagggctaa 5581 ttcactccca acgaatacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc 5641 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa 5701 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga 5761 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc 5821 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt 5881 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag 5941 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg 6001 tctggcatct atgtcgggtg cggagaaaga ggtaatgaaa tggcattatg ggtattatgg 6061 gtctgcatta atgaatcggc caacggatgt cggtgtgaaa taccgcacag atgcgtaagg 6121 agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc 6181 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa 6241 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt 6301 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa 6361 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt 6421 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg 6481 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc 6541 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc 6601 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta 6661 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct 6721 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc 6781 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa 6841 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa 6901 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa 6961 aactcacgtt aagggatttt ggccatgaga ttatcaaaaa ggatcttcac ctagatcctt 7021 ttcacgtaga aagccagtcc gcagaaacgg tgctgacccc ggatgaatgt cagctactgg 7081 gctatctgga caagggaaaa cgcaagcgca aagagaaagc aggtagcttg cagtgggctt 7141 acatggcgat agctagactg ggcggtttta tggacagcaa gcgaaccgga attgccagct 7201 ggggcgccct ctggtaaggt tgggaagccc tgcaaagtaa actggatggc tttcttgccg 7261 ccaaggatct gatggcgcag gggatcaagc tctgatcaag agacaggatg aggatcgttt 7321 cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta 7381 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg 7441 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa 7501 ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct 7561 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg 7621 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca 7681 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat 7741 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac 7801 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc 7861 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa 7921 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag 7981 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc 8041 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt 8101 cttgacgagt tcttctgaat tttgttaaaa tttttgttaa atcagctcat tttttaacca 8161 ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag 8221 tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg 8281 gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt 8341 tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag 8401 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc 8461 gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc 8521 gcttaatgcg ccgctacagg gcgcgtccat tcgccattca ggatcgaatt aattcttaat 8581 taacatcatc aataatatac ctt
1 ttttggattgaagccaatatgataatgagggggtggagtttgtgacgtggcgcggggcgt 60 AAAACCTAACTTCGGTTATACTATTACTCCCCCACCTCAAACACTGCACCGCGCCCCGCA 61 gggaacggggcgggtgacgtagtagtgtggcggaagtgtgatgttgcaagtgtggcggaa 120 CCCTTGCCCCGCCCACTGCATCATCACACCGCCTTCACACTACAACGTTCACACCGCCTT Encap other(162,310)>>> | 121 cacatgtaagcgacggatgtggcaaaagtgacgtttttggtgtgcgccggtgtacacagg 180 GTGTACATTCGCTGCCTACACCGTTTTCACTGCAAAAACCACACGCGGCCACATGTGTCC 181 aagtgacaattttcgcgcggttttaggcggatgttgtagtaaatttgggcgtaaccgagt 240 TTCACTGTTAAAAGCGCGCCAAAATCCGCCTACAACATCATTTAAACCCGCATTGGCTCA 241 aagatttggccattttcgcgggaaaactgaataagaggaagtgaaatctgaataattttg 300 TTCTAAACCGGTAAAAGCGCCCTTTTGACTTATTCTCCTTCACTTTAGACTTATTAAAAC 301 tgttactcatagcgcgtaatactctcatgagcggatacatatttgaatgtatttagaaaa 360 ACAATGAGTATCGCGCATTATGAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTT 361 ataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtaactataa 420 TATTTGTTTATCCCCAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTGCATTGATATT 421 cggtcctaaggtagcgaaaatgtagtcttatgcaatactcttgtagtcttgcaacatggt 480 GCCAGGATTCCATCGCTTTTACATCAGAATACGTTATGAGAACATCAGAACGTTGTACCA 481 aacgatgagttagcaacatgccttacaaggagagaaaaagcaccgtgcatgccgattggt 540 TTGCTACTCAATCGTTGTACGGAATGTTCCTCTCTTTTTCGTGGCACGTACGGCTAACCA 541 ggaagtaaggtggtacgatcgtgccttattaggaaggcaacagacgggtctgacatggat 600 CCTTCATTCCACCATGCTAGCACGGAATAATCCTTCCGTTGTCTGCCCAGACTGTACCTA 601 tggacgaaccactgaattgccgcattgcagagatattgtatttaagtgcctagctcgata 660 ACCTGCTTGGTGACTTAACGGCGTAACGTCTCTATAACATAAATTCACGGATCGAGCTAT HIV-1_5_LTR other(668,848)>>> | 661 cataaacgggtctctctggttagaccagatctgagcctgggagctctctggctaactagg 720 GTATTTGCCCAGAGAGACCAATCTGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCC 721 gaacccactgcttaagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccg 780 CTTGGGTGACGAATTCGGAGTTATTTCGAACGGAACTCACGAAGTTCATCACACACGGGC 781 tctgttgtgtgactctggtaactagagatccctcagacccttttagtcagtgtggaaaat 840 AGACAACACACTGAGACCATTGATCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTA 841 ctctagcagtggcgcccgaacagggacttgaaagcgaaagggaaaccagaggagctctct 900 GAGATCGTCACCGCGGGCTTGTCCCTGAACTTTCGCTTTCCCTTTGGTCTCCTCGAGAGA HIV-1_psi_pack other(959,1003)>>> | 901 cgacgcaggactcggcttgctgaagcgcgcacggcaagaggcgaggggcggcgactggtg 960 GCTGCGTCCTGAGCCGAACGACTTCGCGCGTGCCGTTCTCCGCTCCCCGCCGCTGACCAC 961 agtacgccaaaaattttgactagcggaggctagaaggagagagatgggtgcgagagcgtc 1020 TCATGCGGTTTTTAAAACTGATCGCCTCCGATCTTCCTCTCTCTACCCACGCTCTCGCAG NruI | 1021 agtattaagcgggggagaattagatcgcgatgggaaaaaattcggttaaggccaggggga 1080 TCATAATTCGCCCCCTCTTAATCTAGCGCTACCCTTTTTTAAGCCAATTCCGGTCCCCCT 1081 aagaaaaaatataaattaaaacatatagtatgggcaagcagggagctagaacgattcgca 1140 TTCTTTTTTATATTTAATTTTGTATATCATACCCGTTCGTCCCTCGATCTTGCTAAGCGT 1141 gttaatcctggcctgttagaaacatcagaaggctgtagacaaatactgggacagctacaa 1200 CAATTAGGACCGGACAATCTTTGTAGTCTTCCGACATCTGTTTATGACCCTGTCGATGTT 1201 ccatcccttcagacaggatcagaagaacttagatcattatataatacagtagcaaccctc 1260 GGTAGGGAAGTCTGTCCTAGTCTTCTTGAATCTAGTAATATATTATGTCATCGTTGGGAG 1261 tattgtgtgcatcaaaggatagagataaaagacaccaaggaagctttagacaagatagag 1320 ATAACACACGTAGTTTCCTATCTCTATTTTCTGTGGTTCCTTCGAAATCTGTTCTATCTC 1321 gaagagcaaaacaaaagtaagaccaccgcacagcaagcccgctgatcttcagacctggag 1380 CTTCTCGTTTTGTTTTCATTCTGGTGGCGTGTCGTTCGGGCGACTAGAAGTCTGGACCTC 1381 gaggagatatgagggacattggagaagtgaattatataaatataaagtagtaaaaattga 1440 CTCCTCTATACTCCCTGTAACCTCTTCACTTAATATATTTATATTTCATCATTTTTAACT 1441 accattaggagtagcacccaccaaggcaaagagaagagtggtgcagagagaaaaaagagc 1500 TGGTAATCCTCATCGTGGGTGGTTCCGTTTCTCTTCTCACCACGTCTCTCTTTTTTCTCG RRE reg(1510,1743)>>> ORF_3 rf(2)(1553,2254)>>> | | 1501 agtgggaataggagctttgttccttgggttcttgggagcagcaggaagcactatgggcgc 1560 TCACCCTTATCCTCGAAACAAGGAACCCAAGAACCCTCGTCGTCCTTCGTGATACCCGCG 1561 agcgtcaatgacgctgacggtacaggccagacaattattgtctggtatagtgcagcagca 1620 TCGCAGTTACTGCGACTGCCATGTCCGGTCTGTTAATAACAGACCATATCACGTCGTCGT 1621 gaacaatttgctgagggctattgaggcgcaacagcatctgttgcaactcacagtctgggg 1680 CTTGTTAAACGACTCCCGATAACTCCGCGTTGTCGTAGACAACGTTGAGTGTCAGACCCC 1681 catcaagcagctccaggcaagaatcctggctgtggaaagatacctaaaggatcaacagct 1740 GTAGTTCGTCGAGGTCCGTTCTTAGGACCGACACCTTTCTATGGATTTCCTAGTTGTCGA 1741 cctggggatttggggttgctctggaaaactcatttgcaccactgctgtgccttggaatgc 1800 GGACCCCTAAACCCCAACGAGACCTTTTGAGTAAACGTGGTGACGACACGGAACCTTACG 1801 tagttggagtaataaatctctggaacagatttggaatcacacgacctggatggagtggga 1860 ATCAACCTCATTATTTAGAGACCTTGTCTAAACCTTAGTGTGCTGGACCTACCTCACCCT 1861 cagagaaattaacaattacacaagcttaatacactccttaattgaagaatcgcaaaacca 1920 GTCTCTTTAATTGTTAATGTGTTCGAATTATGTGAGGAATTAACTTCTTAGCGTTTTGGT 1921 gcaagaaaagaatgaacaagaattattggaattagataaatgggcaagtttgtggaattg 1980 CGTTCTTTTCTTACTTGTTCTTAATAACCTTAATCTATTTACCCGTTCAAACACCTTAAC 1981 gtttaacataacaaattggctgtggtatataaaattattcataatgatagtaggaggctt 2040 CAAATTGTATTGTTTAACCGACACCATATATTTTAATAAGTATTACTATCATCCTCCGAA 2041 ggtaggtttaagaatagtttttgctgtactttctatagtgaatagagttaggcagggata 2100 CCATCCAAATTCTTATCAAAAACGACATGAAAGATATCACTTATCTCAATCCGTCCCTAT 2101 ttcaccattatcgtttcagacccacctcccaaccccgaggggacccgacaggcccgaagg 2160 AAGTGGTAATAGCAAAGTCTGGGTGGAGGGTTGGGGCTCCCCTGGGCTGTCCGGGCTTCC 2161 aatagaagaagaaggtggagagagagacagagacagatccattcgattagtgaacggatc 2220 TTATCTTCTTCTTCCACCTCTCTCTCTGTCTCTGTCTAGGTAAGCTAATCACTTGCCTAG 2221 tcgacggtatcgaaagcttgggattcgaatttaaaagaaaaggggggattggggggtaca 2280 AGCTGCCATAGCTTTCGAACCCTAAGCTTAAATTTTCTTTTCCCCCCTAACCCCCCATGT 2281 gtgcaggggaaagaatagtagacataatagcaacagacatacaaactaaagaactacaaa 2340 CACGTCCCCTTTCTTATCATCTGTATTATCGTTGTCTGTATGTTTGATTTCTTGATGTTT hU6 prom(2393,2648)>>> | 2341 aacaaattacaaaaattcaaaattttcgggtttttcgaacctaggtacgtccaaggtcgg 2400 TTGTTTAATGTTTTTAAGTTTTAAAAGCCCAAAAAGCTTGGATCCATGCAGGTTCCAGCC 2401 gcaggaagagggcctatttcccatgattccttcatatttgcatatacgatacaaggctgt 2460 CGTCCTTCTCCCGGATAAAGGGTACTAAGGAAGTATAAACGTATATGCTATGTTCCGACA 2461 tagagagataattagaattaatttgactgtaaacacaaagatattagtacaaaatacgtg 2520 ATCTCTCTATTAATCTTAATTAAACTGACATTTGTGTTTCTATAATCATGTTTTATGCAC 2521 acgtagaaagtaataatttcttgggtagtttgcagttttaaaattatgttttaaaatgga 2580 TGCATCTTTCATTATTAAAGAACCCATCAAACGTCAAAATTTTAATACAAAATTTTACCT 2581 ctatcatatgcttaccgtaacttgaaagtatttcgatttcttggctttatatatcttgtg 2640 GATAGTATACGAATGGCATTGAACTTTCATAAAGCTAAAGAACCGAAATATATAGAACAC XbaI EcoRV XhoI | | | 2641 gaaaggacaaaaatagtcttctctagagatatcatttaaatgtcgacgaattcctcgaga 2700 CTTTCCTGTTTTTATCAGAAGAGATCTCTATAGTAAATTTACAGCTGCTTAAGGAGCTCT 2701 gaagacagctttttttggtgtttcgtggtctcatacagaacttataagattcccaaatcc 2760 CTTCTGTCGAAAAAAACCACAAAGCACCAGAGTATGTCTTGAATATTCTAAGGGTTTAGG 2761 aaagacatttcacgtttatggtgatttcccagaacacatagcgacatgcaaatattgcag 2820 TTTCTGTAAAGTGCAAATACCACTAAAGGGTCTTGTGTATCGCTGTACGTTTATAACGTC 2821 ggcgccactcccctgtccctcacagccatcttcctgccagggcgcacgcgcgctgggtgt 2880 CCGCGGTGAGGGGACAGGGAGTGTCGGTAGAAGGACGGTCCCGCGTGCGCGCGACCCACA ApaI | 2881 tcccgcctagtgacactgggcccgcgattccttggagcgggttgatgacgtcagcgttct 2940 AGGGCGGATCACTGTGACCCGGGCGCTAAGGAACCTCGCCCAACTACTGCAGTCGCAAGA 2941 caattgtagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgtt 3000 GTTAACATCATTAGTTAATGCCCCAGTAATCAAGTATCGGGTATATACCTCAAGGCGCAA 3001 acataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacg 3060 TGTATTGAATGCCATTTACCGGGCGGACCGACTGGCGGGTTGCTGGGGGCGGGTAACTGC 3061 tcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgg 3120 AGTTATTACTGCATACAAGGGTATCATTGCGGTTATCCCTGAAAGGTAACTGCAGTTACC 3121 gtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagt 3180 CACCTCATAAATGCCATTTGACGGGTGAACCGTCATGTAGTTCACATAGTATACGGTTCA 3181 acgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatg 3240 TGCGGGGGATAACTGCAGTTACTGCCATTTACCGGGCGGACCGTAATACGGGTCATGTAC 3241 accttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatg 3300 TGGAATACCCTGAAAGGATGAACCGTCATGTAGATGCATAATCAGTAGCGATAATGGTAC 3301 gtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggattt 3360 CACTACGCCAAAACCGTCATGTAGTTACCCGCACCTATCGCCAAACTGAGTGCCCCTAAA 3361 ccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggac 3420 GGTTCAGAGGTGGGGTAACTGCAGTTACCCTCAAACAAAACCGTGGTTTTAGTTGCCCTG 3421 tttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgtacgg 3480 AAAGGTTTTACAGCATTGTTGAGGCGGGGTAACTGCGTTTACCCGCCATCCGCACATGCC KpnI | 3481 tgggaggtctatataagcagagctggtttagtgaaccgtcagggtaccggatcccttaag 3540 ACCCTCCAGATATATTCGTCTCGACCAAATCACTTGGCAGTCCCATGGCCTAGGGAATTC EGFP reporter(3559,4275)>>> | ORF_1 rf(1)(3559,4959)>>> | 3541 tgaacaactagtgccaccatggtgagcaagggcgaggagctgttcaccggggtggtgccc 3600 ACTTGTTGATCACGGTGGTACCACTCGTTCCCGCTCCTCGACAAGTGGCCCCACCACGGG 3601 atcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggc 3660 TAGGACCAGCTCGACCTGCCGCTGCATTTGCCGGTGTTCAAGTCGCACAGGCCGCTCCCG 3661 gagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctg 3720 CTCCCGCTACGGTGGATGCCGTTCGACTGGGACTTCAAGTAGACGTGGTGGCCGTTCGAC 3721 cccgtgccctggcccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgc 3780 GGGCACGGGACCGGGTGGGAGCACTGGTGGGACTGGATGCCGCACGTCACGAAGTCGGCG 3781 taccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtc 3840 ATGGGGCTGGTGTACTTCGTCGTGCTGAAGAAGTTCAGGCGGTACGGGCTTCCGATGCAG 3841 caggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaag 3900 GTCCTCGCGTGGTAGAAGAAGTTCCTGCTGCCGTTGATGTTCTGGGCGCGGCTCCACTTC 3901 ttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggac 3960 AAGCTCCCGCTGTGGGACCACTTGGCGTAGCTCGACTTCCCGTAGCTGAAGTTCCTCCTG 3961 ggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatg 4020 CCGTTGTAGGACCCCGTGTTCGACCTCATGTTGATGTTGTCGGTGTTGCAGATATAGTAC 4021 gccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggac 4080 CGGCTGTTCGTCTTCTTGCCGTAGTTCCACTTGAAGTTCTAGGCGGTGTTGTAGCTCCTG 4081 ggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtg 4140 CCGTCGCACGTCGAGCGGCTGGTGATGGTCGTCTTGTGGGGGTAGCCGCTGCCGGGGCAC 4141 ctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgag 4200 GACGACGGGCTGTTGGTGATGGACTCGTGGGTCAGGCGGGACTCGTTTCTGGGGTTGCTC 4201 aagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatg 4260 TTCGCGCTAGTGTACCAGGACGACCTCAAGCACTGGCGGCGGCCCTAGTGAGAGCCGTAC 4261 gacgagctgtacaaggaattcggaagcgggcagtgcaccaactacgccctgctgaagctg 4320 CTGCTCGACATGTTCCTTAAGCCTTCGCCCGTCACGTGGTTGATGCGGGACGACTTCGAC puro marker(4363,4959)>>> | 4321 gccggcgacgtggagagcaaccccggccccggatccatggccaccgagtacaagcccacg 4380 CGGCCGCTGCACCTCTCGTTGGGGCCGGGGCCTAGGTACCGGTGGCTCATGTTCGGGTGC SmaI | XmaI | | 4381 gtgcgcctcgccacccgcgacgacgtcccccgggccgtacgcaccctcgccgccgcgttc 4440 CACGCGGAGCGGTGGGCGCTGCTGCAGGGGGCCCGGCATGCGTGGGAGCGGCGGCGCAAG 4441 gccgactaccccgccacgcgccacaccgtcgacccggaccgccacatcgagcgggtcacc 4500 CGGCTGATGGGGCGGTGCGCGGTGTGGCAGCTGGGCCTGGCGGTGTAGCTCGCCCAGTGG 4501 gagctgcaagaactcttcctcacgcgcgtcgggctcgacatcggcaaggtgtgggtcgcg 4560 CTCGACGTTCTTGAGAAGGAGTGCGCGCAGCCCGAGCTGTAGCCGTTCCACACCCAGCGC 4561 gacgacggcgccgcggtggcggtctggaccacgccggagagcgtcgaagcgggggcggtg 4620 CTGCTGCCGCGGCGCCACCGCCAGACCTGGTGCGGCCTCTCGCAGCTTCGCCCCCGCCAC 4621 ttcgccgagatcggctcgcgcatggccgagttgagcggttcccggctggccgcgcagcaa 4680 AAGCGGCTCTAGCCGAGCGCGTACCGGCTCAACTCGCCAAGGGCCGACCGGCGCGTCGTT StuI | 4681 cagatggaaggcctcctggcgccgcaccggcccaaggagcccgcgtggttcctggccacc 4740 GTCTACCTTCCGGAGGACCGCGGCGTGGCCGGGTTCCTCGGGCGCACCAAGGACCGGTGG 4741 gtcggcgtctcgcccgaccaccagggcaagggtctgggcagcgccgtcgtgctccccgga 4800 CAGCCGCAGAGCGGGCTGGTGGTCCCGTTCCCAGACCCGTCGCGGCAGCACGAGGGGCCT 4801 gtggaggcggccgagcgcgctggggtgcccgccttcctggagacctccgcgccccgcaac 4860 CACCTCCGCCGGCTCGCGCGACCCCACGGGCGGAAGGACCTCTGGAGGCGCGGGGCGTTG 4861 ctccccttctacgagcggctcggcttcaccgtcaccgccgacgtcgaggtgcccgaagga 4920 GAGGGGAAGATGCTCGCCGAGCCGAAGTGGCAGTGGCGGCTGCAGCTCCACGGGCTTCCT 4921 ccgcgcacctggtgcatgacccgcaagcccggtgcctgaacgcgttccggaaatcaacct 4980 GGCGCGTGGACCACGTACTGGGCGTTCGGGCCACGGACTTGCGCAAGGCCTTTAGTTGGA 4981 ctggattacaaaatttgtgaaagattgactggtattcttaactatgttgctccttttacg 5040 GACCTAATGTTTTAAACACTTTCTAACTGACCATAAGAATTGATACAACGAGGAAAATGC 5041 ctatgtggatacgctgctttaatgcctttgtatcatgctattgcttcccgtatggctttc 5100 GATACACCTATGCGACGAAATTACGGAAACATAGTACGATAACGAAGGGCATACCGAAAG 5101 attttctcctccttgtataaatcctggttgctgtctctttatgaggagttgtggcccgtt 5160 TAAAAGAGGAGGAACATATTTAGGACCAACGACAGAGAAATACTCCTCAACACCGGGCAA 5161 gtcaggcaacgtggcgtggtgtgcactgtgtttgctgacgcaacccccactggttggggc 5220 CAGTCCGTTGCACCGCACCACACGTGACACAAACGACTGCGTTGGGGGTGACCAACCCCG 5221 attgccaccacctgtcagctcctttccgggactttcgctttccccctccctattgccacg 5280 TAACGGTGGTGGACAGTCGAGGAAAGGCCCTGAAAGCGAAAGGGGGAGGGATAACGGTGC 5281 gcggaactcatcgccgcctgccttgcccgctgctggacaggggctcggctgttgggcact 5340 CGCCTTGAGTAGCGGCGGACGGAACGGGCGACGACCTGTCCCCGAGCCGACAACCCGTGA 5341 gacaattccgtggtgttgtcggggaagctgacgtcctttccatggctgctcgcctgtgtt 5400 CTGTTAAGGCACCACAACAGCCCCTTCGACTGCAGGAAAGGTACCGACGAGCGGACACAA 5401 gccacctggattctgcgcgggacgtccttctgctacgtcccttcggccctcaatccagcg 5460 CGGTGGACCTAAGACGCGCCCTGCAGGAAGACGATGCAGGGAAGCCGGGAGTTAGGTCGC 5461 gaccttccttcccgcggcctgctgccggctctgcggcctcttccgcgtctcgccttcgcc 5520 CTGGAAGGAAGGGCGCCGGACGACGGCCGAGACGCCGGAGAAGGCGCAGAGCGGAAGCGG delta_U3 other(5569,5621)>>> | 5521 ctcagacgagtcggatctccctttgggccgcctccccgcctgtccggatggaagggctaa 5580 GAGTCTGCTCAGCCTAGAGGGAAACCCGGCGGAGGGGCGGACAGGCCTACCTTCCCGATT HIV-1_5_LTR other(5622,5802)>>> | 5581 ttcactcccaacgaatacaagatctgctttttgcttgtactgggtctctctggttagacc 5640 AAGTGAGGGTTGCTTATGTTCTAGACGAAAAACGAACATGACCCAGAGAGACCAATCTGG 5641 agatctgagcctgggagctctctggctaactagggaacccactgcttaagcctcaataaa 5700 TCTAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAATTCGGAGTTATTT 5701 gcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaactaga 5760 CGAACGGAACTCACGAAGTTCATCACACACGGGCAGACAACACACTGAGACCATTGATCT 5761 gatccctcagacccttttagtcagtgtggaaaatctctagcagtagtagttcatgtcatc 5820 CTAGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCATCATCAAGTACAGTAG 5821 ttattattcagtatttataacttgcaaagaaatgaatatcagagagtgagaggaacttgt 5880 AATAATAAGTCATAAATATTGAACGTTTCTTTACTTATAGTCTCTCACTCTCCTTGAACA 5881 ttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaag 5940 AATAACGTCGAATATTACCAATGTTTATTTCGTTATCGTAGTGTTTAAAGTGTTTATTTC 5941 catttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatg 6000 GTAAAAAAAGTGACGTAAGATCAACACCAAACAGGTTTGAGTAGTTACATAGAATAGTAC 6001 tctggcatctatgtcgggtgcggagaaagaggtaatgaaatggcattatgggtattatgg 6060 AGACCGTAGATACAGCCCACGCCTCTTTCTCCATTACTTTACCGTAATACCCATAATACC 6061 gtctgcattaatgaatcggccaacggatgtcggtgtgaaataccgcacagatgcgtaagg 6120 CAGACGTAATTACTTAGCCGGTTGCCTACAGCCACACTTTATGGCGTGTCTACGCATTCC 6121 agaaaataccgcatcaggcgctcttccgcttcctcgctcactgactcgctgcgctcggtc 6180 TCTTTTATGGCGTAGTCCGCGAGAAGGCGAAGGAGCGAGTGACTGAGCGACGCGAGCCAG 6181 gttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaa 6240 CAAGCCGACGCCGCTCGCCATAGTCGAGTGAGTTTCCGCCATTATGCCAATAGGTGTCTT 6241 tcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgt 6300 AGTCCCCTATTGCGTCCTTTCTTGTACACTCGTTTTCCGGTCGTTTTCCGGTCCTTGGCA pBR322 origin(6309,6928)<<< | 6301 aaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaa 6360 TTTTTCCGGCGCAACGACCGCAAAAAGGTATCCGAGGCGGGGGGACTGCTCGTAGTGTTT 6361 aatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgttt 6420 TTAGCTGCGAGTTCAGTCTCCACCGCTTTGGGCTGTCCTGATATTTCTATGGTCCGCAAA 6421 ccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctg 6480 GGGGGACCTTCGAGGGAGCACGCGAGAGGACAAGGCTGGGACGGCGAATGGCCTATGGAC 6481 tccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctc 6540 AGGCGGAAAGAGGGAAGCCCTTCGCACCGCGAAAGAGTATCGAGTGCGACATCCATAGAG 6541 agttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagccc 6600 TCAAGCCACATCCAGCAAGCGAGGTTCGACCCGACACACGTGCTTGGGGGGCAAGTCGGG 6601 gaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgactta 6660 CTGGCGACGCGGAATAGGCCATTGATAGCAGAACTCAGGTTGGGCCATTCTGTGCTGAAT 6661 tcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgct 6720 AGCGGTGACCGTCGTCGGTGACCATTGTCCTAATCGTCTCGCTCCATACATCCGCCACGA 6721 acagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatc 6780 TGTCTCAAGAACTTCACCACCGGATTGATGCCGATGTGATCTTCCTGTCATAAACCATAG 6781 tgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaa 6840 ACGCGAGACGACTTCGGTCAATGGAAGCCTTTTTCTCAACCATCGAGAACTAGGCCGTTT 6841 caaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaa 6900 GTTTGGTGGCGACCATCGCCACCAAAAAAACAAACGTTCGTCGTCTAATGCGCGTCTTTT 6901 aaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaa 6960 TTTCCTAGAGTTCTTCTAGGAAACTAGAAAAGATGCCCCAGACTGCGAGTCACCTTGCTT 6961 aactcacgttaagggattttggccatgagattatcaaaaaggatcttcacctagatcctt 7020 TTGAGTGCAATTCCCTAAAACCGGTACTCTAATAGTTTTTCCTAGAAGTGGATCTAGGAA 7021 ttcacgtagaaagccagtccgcagaaacggtgctgaccccggatgaatgtcagctactgg 7080 AAGTGCATCTTTCGGTCAGGCGTCTTTGCCACGACTGGGGCCTACTTACAGTCGATGACC 7081 gctatctggacaagggaaaacgcaagcgcaaagagaaagcaggtagcttgcagtgggctt 7140 CGATAGACCTGTTCCCTTTTGCGTTCGCGTTTCTCTTTCGTCCATCGAACGTCACCCGAA NEOKAN prom(7186,7235)>>> | 7141 acatggcgatagctagactgggcggttttatggacagcaagcgaaccggaattgccagct 7200 TGTACCGCTATCGATCTGACCCGCCAAAATACCTGTCGTTCGCTTGGCCTTAACGGTCGA 7201 ggggcgccctctggtaaggttgggaagccctgcaaagtaaactggatggctttcttgccg 7260 CCCCGCGGGAGACCATTCCAACCCTTCGGGACGTTTCATTTGACCTACCGAAAGAACGGC BclI | 7261 ccaaggatctgatggcgcaggggatcaagctctgatcaagagacaggatgaggatcgttt 7320 GGTTCCTAGACTACCGCGTCCCCTAGTTCGAGACTAGTTCTCTGTCCTACTCCTAGCAAA NTP_II marker(7327,8115)>>> | ORF_2 rf(1)(7324,8118)>>> | | 7321 cgcatgattgaacaagatggattgcacgcaggttctccggccgcttgggtggagaggcta 7380 GCGTACTAACTTGTTCTACCTAACGTGCGTCCAAGAGGCCGGCGAACCCACCTCTCCGAT 7381 ttcggctatgactgggcacaacagacaatcggctgctctgatgccgccgtgttccggctg 7440 AAGCCGATACTGACCCGTGTTGTCTGTTAGCCGACGAGACTACGGCGGCACAAGGCCGAC 7441 tcagcgcaggggcgcccggttctttttgtcaagaccgacctgtccggtgccctgaatgaa 7500 AGTCGCGTCCCCGCGGGCCAAGAAAAACAGTTCTGGCTGGACAGGCCACGGGACTTACTT 7501 ctgcaagacgaggcagcgcggctatcgtggctggccacgacgggcgttccttgcgcagct 7560 GACGTTCTGCTCCGTCGCGCCGATAGCACCGACCGGTGCTGCCCGCAAGGAACGCGTCGA 7561 gtgctcgacgttgtcactgaagcgggaagggactggctgctattgggcgaagtgccgggg 7620 CACGAGCTGCAACAGTGACTTCGCCCTTCCCTGACCGACGATAACCCGCTTCACGGCCCC 7621 caggatctcctgtcatctcaccttgctcctgccgagaaagtatccatcatggctgatgca 7680 GTCCTAGAGGACAGTAGAGTGGAACGAGGACGGCTCTTTCATAGGTAGTACCGACTACGT 7681 atgcggcggctgcatacgcttgatccggctacctgcccattcgaccaccaagcgaaacat 7740 TACGCCGCCGACGTATGCGAACTAGGCCGATGGACGGGTAAGCTGGTGGTTCGCTTTGTA 7741 cgcatcgagcgagcacgtactcggatggaagccggtcttgtcgatcaggatgatctggac 7800 GCGTAGCTCGCTCGTGCATGAGCCTACCTTCGGCCAGAACAGCTAGTCCTACTAGACCTG 7801 gaagagcatcaggggctcgcgccagccgaactgttcgccaggctcaaggcgagcatgccc 7860 CTTCTCGTAGTCCCCGAGCGCGGTCGGCTTGACAAGCGGTCCGAGTTCCGCTCGTACGGG 7861 gacggcgaggatctcgtcgtgacccatggcgatgcctgcttgccgaatatcatggtggaa 7920 CTGCCGCTCCTAGAGCAGCACTGGGTACCGCTACGGACGAACGGCTTATAGTACCACCTT 7921 aatggccgcttttctggattcatcgactgtggccggctgggtgtggcggaccgctatcag 7980 TTACCGGCGAAAAGACCTAAGTAGCTGACACCGGCCGACCCACACCGCCTGGCGATAGTC 7981 gacatagcgttggctacccgtgatattgctgaagagcttggcggcgaatgggctgaccgc 8040 CTGTATCGCAACCGATGGGCACTATAACGACTTCTCGAACCGCCGCTTACCCGACTGGCG 8041 ttcctcgtgctttacggtatcgccgctcccgattcgcagcgcatcgccttctatcgcctt 8100 AAGGAGCACGAAATGCCATAGCGGCGAGGGCTAAGCGTCGCGTAGCGGAAGATAGCGGAA 8101 cttgacgagttcttctgaattttgttaaaatttttgttaaatcagctcattttttaacca 8160 GAACTGCTCAAGAAGACTTAAAACAATTTTAAAAACAATTTAGTCGAGTAAAAAATTGGT 8161 ataggccgaaatcggcaaaatcccttataaatcaaaagaatagaccgagatagggttgag 8220 TATCCGGCTTTAGCCGTTTTAGGGAATATTTAGTTTTCTTATCTGGCTCTATCCCAACTC f1 origin(8223,8529)<<< | 8221 tgttgttccagtttggaacaagagtccactattaaagaacgtggactccaacgtcaaagg 8280 ACAACAAGGTCAAACCTTGTTCTCAGGTGATAATTTCTTGCACCTGAGGTTGCAGTTTCC 8281 gcgaaaaaccgtctatcagggcgatggcccactacgtgaaccatcaccctaatcaagttt 8340 CGCTTTTTGGCAGATAGTCCCGCTACCGGGTGATGCACTTGGTAGTGGGATTAGTTCAAA 8341 tttggggtcgaggtgccgtaaagcactaaatcggaaccctaaagggagcccccgatttag 8400 AAACCCCAGCTCCACGGCATTTCGTGATTTAGCCTTGGGATTTCCCTCGGGGGCTAAATC 8401 agcttgacggggaaagccggcgaacgtggcgagaaaggaagggaagaaagcgaaaggagc 8460 TCGAACTGCCCCTTTCGGCCGCTTGCACCGCTCTTTCCTTCCCTTCTTTCGCTTTCCTCG 8461 gggcgctagggcgctggcaagtgtagcggtcacgctgcgcgtaaccaccacacccgccgc 8520 CCCGCGATCCCGCGACCGTTCACATCGCCAGTGCGACGCGCATTGGTGGTGTGGGCGGCG 8521 gcttaatgcgccgctacagggcgcgtccattcgccattcaggatcgaattaattcttaat 8580 CGAATTACGCGGCGATGTCCCGCGCAGGTAAGCGGTAAGTCCTAGCTTAATTAAGAATTA 8581 taacatcatcaataatatacctt 8603 ATTGTAGTAGTTATTATATGGAA
***Note: The text map shown below is representative of the parental
vector only and does not include the gene of interest. The sequence of the gene
can be found through the accession number link on the product page.
LOCUS dna 8603 bp FEATURES Location/Qualifiers Other Gene 162..310 /gene="Encap other" Other Gene 668..848 /gene="HIV-1_5_LTR other" Other Gene 959..1003 /gene="HIV-1_psi_pack other" misc_binding 1045..1050 /dbxref="REBASE:NruI" Regulatory_Seq 1510..1743 /gene="RRE reg" ORF 1553..2254 /sequence="ORF_3 rf(2)" Promoter 2393..2648 /gene="hU6 prom" misc_binding 2662..2667 /dbxref="REBASE:XbaI" misc_binding 2668..2673 /dbxref="REBASE:EcoRV" misc_binding 2694..2699 /dbxref="REBASE:XhoI" misc_binding 2898..2903 /dbxref="REBASE:ApaI" misc_binding 3523..3528 /dbxref="REBASE:KpnI" Reporter 3559..4275 /gene="EGFP reporter" ORF 3559..4959 /sequence="ORF_1 rf(1)" Marker 4363..4959 /gene="puro marker" misc_binding 4409..4414 /dbxref="REBASE:XmaI" misc_binding 4409..4414 /dbxref="REBASE:SmaI" misc_binding 4689..4694 /dbxref="REBASE:StuI" Other Gene 5569..5621 /gene="delta_U3 other" Other Gene 5622..5802 /gene="HIV-1_5_LTR other" Rep_Origin 6309..6928 /gene="pBR322 origin" Promoter 7186..7235 /gene="NEOKAN prom" misc_binding 7293..7298 /dbxref="REBASE:BclI" ORF 7324..8118 /sequence="ORF_2 rf(1)" Marker 7327..8115 /gene="NTP_II marker" Rep_Origin 8223..8529 /gene="f1 origin" BASE COUNT 2173 a 2132 c 2344 g 1954 t 0 others ORIGIN 1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt 61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa 121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg 181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt 241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg 301 tgttactcat agcgcgtaat actctcatga gcggatacat atttgaatgt atttagaaaa 361 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtaactataa 421 cggtcctaag gtagcgaaaa tgtagtctta tgcaatactc ttgtagtctt gcaacatggt 481 aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt 541 ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat 601 tggacgaacc actgaattgc cgcattgcag agatattgta tttaagtgcc tagctcgata 661 cataaacggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg 721 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg 781 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat 841 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct 901 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg 961 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc 1021 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga 1081 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca 1141 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa 1201 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc 1261 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag 1321 gaagagcaaa acaaaagtaa gaccaccgca cagcaagccc gctgatcttc agacctggag 1381 gaggagatat gagggacatt ggagaagtga attatataaa tataaagtag taaaaattga 1441 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc 1501 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc 1561 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca 1621 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg 1681 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct 1741 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc 1801 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga 1861 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca 1921 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg 1981 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt 2041 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata 2101 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg 2161 aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc 2221 tcgacggtat cgaaagcttg ggattcgaat ttaaaagaaa aggggggatt ggggggtaca 2281 gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa gaactacaaa 2341 aacaaattac aaaaattcaa aattttcggg tttttcgaac ctaggtacgt ccaaggtcgg 2401 gcaggaagag ggcctatttc ccatgattcc ttcatatttg catatacgat acaaggctgt 2461 tagagagata attagaatta atttgactgt aaacacaaag atattagtac aaaatacgtg 2521 acgtagaaag taataatttc ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga 2581 ctatcatatg cttaccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg 2641 gaaaggacaa aaatagtctt ctctagagat atcatttaaa tgtcgacgaa ttcctcgaga 2701 gaagacagct ttttttggtg tttcgtggtc tcatacagaa cttataagat tcccaaatcc 2761 aaagacattt cacgtttatg gtgatttccc agaacacata gcgacatgca aatattgcag 2821 ggcgccactc ccctgtccct cacagccatc ttcctgccag ggcgcacgcg cgctgggtgt 2881 tcccgcctag tgacactggg cccgcgattc cttggagcgg gttgatgacg tcagcgttct 2941 caattgtagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt 3001 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg 3061 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg 3121 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt 3181 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg 3241 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg 3301 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt 3361 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac 3421 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg 3481 tgggaggtct atataagcag agctggttta gtgaaccgtc agggtaccgg atcccttaag 3541 tgaacaacta gtgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc 3601 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc 3661 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg 3721 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc 3781 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc 3841 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag 3901 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac 3961 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg 4021 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac 4081 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg 4141 ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag 4201 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg 4261 gacgagctgt acaaggaatt cggaagcggg cagtgcacca actacgccct gctgaagctg 4321 gccggcgacg tggagagcaa ccccggcccc ggatccatgg ccaccgagta caagcccacg 4381 gtgcgcctcg ccacccgcga cgacgtcccc cgggccgtac gcaccctcgc cgccgcgttc 4441 gccgactacc ccgccacgcg ccacaccgtc gacccggacc gccacatcga gcgggtcacc 4501 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg 4561 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg 4621 ttcgccgaga tcggctcgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa 4681 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc 4741 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga 4801 gtggaggcgg ccgagcgcgc tggggtgccc gccttcctgg agacctccgc gccccgcaac 4861 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga 4921 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgaa cgcgttccgg aaatcaacct 4981 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg 5041 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc 5101 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt 5161 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc 5221 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg 5281 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact 5341 gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt 5401 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg 5461 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct cgccttcgcc 5521 ctcagacgag tcggatctcc ctttgggccg cctccccgcc tgtccggatg gaagggctaa 5581 ttcactccca acgaatacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc 5641 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa 5701 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga 5761 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc 5821 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt 5881 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag 5941 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg 6001 tctggcatct atgtcgggtg cggagaaaga ggtaatgaaa tggcattatg ggtattatgg 6061 gtctgcatta atgaatcggc caacggatgt cggtgtgaaa taccgcacag atgcgtaagg 6121 agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc 6181 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa 6241 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt 6301 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa 6361 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt 6421 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg 6481 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc 6541 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc 6601 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta 6661 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct 6721 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc 6781 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa 6841 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa 6901 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa 6961 aactcacgtt aagggatttt ggccatgaga ttatcaaaaa ggatcttcac ctagatcctt 7021 ttcacgtaga aagccagtcc gcagaaacgg tgctgacccc ggatgaatgt cagctactgg 7081 gctatctgga caagggaaaa cgcaagcgca aagagaaagc aggtagcttg cagtgggctt 7141 acatggcgat agctagactg ggcggtttta tggacagcaa gcgaaccgga attgccagct 7201 ggggcgccct ctggtaaggt tgggaagccc tgcaaagtaa actggatggc tttcttgccg 7261 ccaaggatct gatggcgcag gggatcaagc tctgatcaag agacaggatg aggatcgttt 7321 cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta 7381 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg 7441 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa 7501 ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct 7561 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg 7621 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca 7681 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat 7741 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac 7801 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc 7861 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa 7921 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag 7981 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc 8041 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt 8101 cttgacgagt tcttctgaat tttgttaaaa tttttgttaa atcagctcat tttttaacca 8161 ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag 8221 tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg 8281 gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt 8341 tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag 8401 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc 8461 gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc 8521 gcttaatgcg ccgctacagg gcgcgtccat tcgccattca ggatcgaatt aattcttaat 8581 taacatcatc aataatatac ctt //
LOCUS dna 8603 bp FEATURES Location/Qualifiers Other Gene 162..310 /gene="Encap other" Other Gene 668..848 /gene="HIV-1_5_LTR other" Other Gene 959..1003 /gene="HIV-1_psi_pack other" misc_binding 1045..1050 /dbxref="REBASE:NruI" Regulatory_Seq 1510..1743 /gene="RRE reg" ORF 1553..2254 /sequence="ORF_3 rf(2)" Promoter 2393..2648 /gene="hU6 prom" misc_binding 2662..2667 /dbxref="REBASE:XbaI" misc_binding 2668..2673 /dbxref="REBASE:EcoRV" misc_binding 2694..2699 /dbxref="REBASE:XhoI" misc_binding 2898..2903 /dbxref="REBASE:ApaI" misc_binding 3523..3528 /dbxref="REBASE:KpnI" Reporter 3559..4275 /gene="EGFP reporter" ORF 3559..4959 /sequence="ORF_1 rf(1)" Marker 4363..4959 /gene="puro marker" misc_binding 4409..4414 /dbxref="REBASE:XmaI" misc_binding 4409..4414 /dbxref="REBASE:SmaI" misc_binding 4689..4694 /dbxref="REBASE:StuI" Other Gene 5569..5621 /gene="delta_U3 other" Other Gene 5622..5802 /gene="HIV-1_5_LTR other" Rep_Origin 6309..6928 /gene="pBR322 origin" Promoter 7186..7235 /gene="NEOKAN prom" misc_binding 7293..7298 /dbxref="REBASE:BclI" ORF 7324..8118 /sequence="ORF_2 rf(1)" Marker 7327..8115 /gene="NTP_II marker" Rep_Origin 8223..8529 /gene="f1 origin" BASE COUNT 2173 a 2132 c 2344 g 1954 t 0 others