piLenti-shRNA-GFP
Vector Name | piLenti-shRNA-GFP |
VectorType | Lentiviral siRNA Vector |
Antibiotic Information | Bacterial: Kanamycin Mammalian: Puromycin |
Sequencing Primers | 5’--TACGTCCAAGGTCGGGCAGGAAGA--3’ in the U6 promoter region |
Additional Information | Sequence 8373 BP; 2111 A; 2057 C; 2281 G; 1882 T; 42 other; ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt 60 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa 120 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg 180 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt 240 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg 300 tgttactcat agcgcgtaat actctcatga gcggatacat atttgaatgt atttagaaaa 360 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtaactataa 420 cggtcctaag gtagcgaaaa tgtagtctta tgcaatactc ttgtagtctt gcaacatggt 480 aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt 540 ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat 600 tggacgaacc actgaattgc cgcattgcag agatattgta tttaagtgcc tagctcgata 660 cataaacggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg 720 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg 780 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat 840 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct 900 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg 960 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc 1020 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga 1080 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca 1140 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa 1200 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc 1260 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag 1320 gaagagcaaa acaaaagtaa gaccaccgca cagcaagccc gctgatcttc agacctggag 1380 gaggagatat gagggacatt ggagaagtga attatataaa tataaagtag taaaaattga 1440 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc 1500 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc 1560 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca 1620 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg 1680 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct 1740 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc 1800 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga 1860 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca 1920 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg 1980 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt 2040 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata 2100 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg 2160 aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc 2220 tcgacggtat cgaaagcttg ggattcgaat ttaaaagaaa aggggggatt ggggggtaca 2280 gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa gaactacaaa 2340 aacaaattac aaaaattcaa aattttcggg tttttcgaac ctaggtacgt ccaaggtcgg 2400 gcaggaagag ggcctatttc ccatgattcc ttcatatttg catatacgat acaaggctgt 2460 tagagagata attagaatta atttgactgt aaacacaaag atattagtac aaaatacgtg 2520 acgtagaaag taataatttc ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga 2580 ctatcatatg cttaccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg 2640 gaaaggacaA AAANNNNNNN NNNNNNNNNN NNNNCGAANN NNNNNNNNNN NNNNNNNNNT 2700 TTTTTGGTAC CAATTgtagt aatcaattac ggggtcatta gttcatagcc catatatgga 2760 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg 2820 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg 2880 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca 2940 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc 3000 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc 3060 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc 3120 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa 3180 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag 3240 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agggtaccgg 3300 atcccttaag tgaacaacta gtgccaccat ggtgagcaag ggcgaggagc tgttcaccgg 3360 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc 3420 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac 3480 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg 3540 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga 3600 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc 3660 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt 3720 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt 3780 ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa 3840 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga 3900 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga 3960 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac 4020 tctcggcatg gacgagctgt acaaggaatt cggaagcggg cagtgcacca actacgccct 4080 gctgaagctg gccggcgacg tggagagcaa ccccggcccc ggatccatgg ccaccgagta 4140 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc cgggccgtac gcaccctcgc 4200 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gacccggacc gccacatcga 4260 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt 4320 gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc 4380 gggggcggtg ttcgccgaga tcggctcgcg catggccgag ttgagcggtt cccggctggc 4440 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt 4500 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt 4560 gctccccgga gtggaggcgg ccgagcgcgc tggggtgccc gccttcctgg agacctccgc 4620 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt 4680 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgaa cgcgttccgg 4740 aaatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc 4800 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg 4860 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt 4920 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac 4980 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc 5040 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct 5100 gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct 5160 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct 5220 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct 5280 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc tgtccggatg 5340 gaagggctaa ttcactccca acgaatacaa gatctgcttt ttgcttgtac tgggtctctc 5400 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag 5460 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct 5520 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt 5580 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag 5640 aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc 5700 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta 5760 tcttatcatg tctggcatct atgtcgggtg cggagaaaga ggtaatgaaa tggcattatg 5820 ggtattatgg gtctgcatta atgaatcggc caacggatgt cggtgtgaaa taccgcacag 5880 atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct 5940 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt 6000 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc 6060 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga 6120 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata 6180 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac 6240 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg 6300 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc 6360 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag 6420 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt 6480 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt 6540 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg 6600 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac 6660 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca 6720 gtggaacgaa aactcacgtt aagggatttt ggccatgaga ttatcaaaaa ggatcttcac 6780 ctagatcctt ttcacgtaga aagccagtcc gcagaaacgg tgctgacccc ggatgaatgt 6840 cagctactgg gctatctgga caagggaaaa cgcaagcgca aagagaaagc aggtagcttg 6900 cagtgggctt acatggcgat agctagactg ggcggtttta tggacagcaa gcgaaccgga 6960 attgccagct ggggcgccct ctggtaaggt tgggaagccc tgcaaagtaa actggatggc 7020 tttcttgccg ccaaggatct gatggcgcag gggatcaagc tctgatcaag agacaggatg 7080 aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt 7140 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt 7200 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc 7260 cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc 7320 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga 7380 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat 7440 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca 7500 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga 7560 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc 7620 gagcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat 7680 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga 7740 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg 7800 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt 7860 ctatcgcctt cttgacgagt tcttctgaat tttgttaaaa tttttgttaa atcagctcat 7920 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga 7980 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca 8040 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct 8100 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc 8160 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag 8220 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca 8280 cacccgccgc gcttaatgcg ccgctacagg gcgcgtccat tcgccattca ggatcgaatt 8340 aattcttaat taacatcatc aataatatac ctt 8373 |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
Sequence 8373 BP; 2111 A; 2057 C; 2281 G; 1882 T; 42 other; ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt 60 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa 120 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg 180 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt 240 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg 300 tgttactcat agcgcgtaat actctcatga gcggatacat atttgaatgt atttagaaaa 360 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtaactataa 420 cggtcctaag gtagcgaaaa tgtagtctta tgcaatactc ttgtagtctt gcaacatggt 480 aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt 540 ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat 600 tggacgaacc actgaattgc cgcattgcag agatattgta tttaagtgcc tagctcgata 660 cataaacggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg 720 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg 780 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat 840 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct 900 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg 960 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc 1020 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga 1080 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca 1140 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa 1200 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc 1260 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag 1320 gaagagcaaa acaaaagtaa gaccaccgca cagcaagccc gctgatcttc agacctggag 1380 gaggagatat gagggacatt ggagaagtga attatataaa tataaagtag taaaaattga 1440 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc 1500 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc 1560 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca 1620 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg 1680 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct 1740 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc 1800 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga 1860 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca 1920 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg 1980 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt 2040 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata 2100 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg 2160 aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc 2220 tcgacggtat cgaaagcttg ggattcgaat ttaaaagaaa aggggggatt ggggggtaca 2280 gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa gaactacaaa 2340 aacaaattac aaaaattcaa aattttcggg tttttcgaac ctaggtacgt ccaaggtcgg 2400 gcaggaagag ggcctatttc ccatgattcc ttcatatttg catatacgat acaaggctgt 2460 tagagagata attagaatta atttgactgt aaacacaaag atattagtac aaaatacgtg 2520 acgtagaaag taataatttc ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga 2580 ctatcatatg cttaccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg 2640 gaaaggacaA AAANNNNNNN NNNNNNNNNN NNNNCGAANN NNNNNNNNNN NNNNNNNNNT 2700 TTTTTGGTAC CAATTgtagt aatcaattac ggggtcatta gttcatagcc catatatgga 2760 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg 2820 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg 2880 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca 2940 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc 3000 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc 3060 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc 3120 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa 3180 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag 3240 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agggtaccgg 3300 atcccttaag tgaacaacta gtgccaccat ggtgagcaag ggcgaggagc tgttcaccgg 3360 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc 3420 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac 3480 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg 3540 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga 3600 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc 3660 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt 3720 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt 3780 ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa 3840 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga 3900 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga 3960 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac 4020 tctcggcatg gacgagctgt acaaggaatt cggaagcggg cagtgcacca actacgccct 4080 gctgaagctg gccggcgacg tggagagcaa ccccggcccc ggatccatgg ccaccgagta 4140 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc cgggccgtac gcaccctcgc 4200 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gacccggacc gccacatcga 4260 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt 4320 gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc 4380 gggggcggtg ttcgccgaga tcggctcgcg catggccgag ttgagcggtt cccggctggc 4440 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt 4500 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt 4560 gctccccgga gtggaggcgg ccgagcgcgc tggggtgccc gccttcctgg agacctccgc 4620 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt 4680 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgaa cgcgttccgg 4740 aaatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc 4800 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg 4860 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt 4920 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac 4980 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc 5040 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct 5100 gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct 5160 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct 5220 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct 5280 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc tgtccggatg 5340 gaagggctaa ttcactccca acgaatacaa gatctgcttt ttgcttgtac tgggtctctc 5400 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag 5460 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct 5520 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt 5580 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag 5640 aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc 5700 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta 5760 tcttatcatg tctggcatct atgtcgggtg cggagaaaga ggtaatgaaa tggcattatg 5820 ggtattatgg gtctgcatta atgaatcggc caacggatgt cggtgtgaaa taccgcacag 5880 atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct 5940 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt 6000 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc 6060 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga 6120 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata 6180 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac 6240 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg 6300 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc 6360 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag 6420 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt 6480 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt 6540 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg 6600 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac 6660 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca 6720 gtggaacgaa aactcacgtt aagggatttt ggccatgaga ttatcaaaaa ggatcttcac 6780 ctagatcctt ttcacgtaga aagccagtcc gcagaaacgg tgctgacccc ggatgaatgt 6840 cagctactgg gctatctgga caagggaaaa cgcaagcgca aagagaaagc aggtagcttg 6900 cagtgggctt acatggcgat agctagactg ggcggtttta tggacagcaa gcgaaccgga 6960 attgccagct ggggcgccct ctggtaaggt tgggaagccc tgcaaagtaa actggatggc 7020 tttcttgccg ccaaggatct gatggcgcag gggatcaagc tctgatcaag agacaggatg 7080 aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt 7140 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt 7200 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc 7260 cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc 7320 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga 7380 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat 7440 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca 7500 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga 7560 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc 7620 gagcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat 7680 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga 7740 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg 7800 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt 7860 ctatcgcctt cttgacgagt tcttctgaat tttgttaaaa tttttgttaa atcagctcat 7920 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga 7980 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca 8040 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct 8100 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc 8160 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag 8220 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca 8280 cacccgccgc gcttaatgcg ccgctacagg gcgcgtccat tcgccattca ggatcgaatt 8340 aattcttaat taacatcatc aataatatac ctt 8373