piLenti-shRNA

Vector Name piLenti-shRNA
VectorType Lentiviral siRNA Vector
Antibiotic Information Bacterial: Kanamycin
Mammalian: Puromycin
Sequencing Primers 5’--TACGTCCAAGGTCGGGCAGGAAGA--3’ in the U6 promoter region
Additional Information No Additional Information
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector only and does not include the gene of interest. The sequence of the gene can be found through the accession number link on the product page. The restriction sites within the cloning region may differ from the provided sequence.
     Sequence 7551 BP; 1914 A; 1782 C; 2047 G; 1766 T; 42 other;
     ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt        60
     gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa       120
     cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg       180
     aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt       240
     aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg       300
     tgttactcat agcgcgtaat actctcatga gcggatacat atttgaatgt atttagaaaa       360
     ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtaactataa       420
     cggtcctaag gtagcgaaaa tgtagtctta tgcaatactc ttgtagtctt gcaacatggt       480
     aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt       540
     ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat       600
     tggacgaacc actgaattgc cgcattgcag agatattgta tttaagtgcc tagctcgata       660
     cataaacggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg       720
     gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg       780
     tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat       840
     ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct       900
     cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg       960
     agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc      1020
     agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga      1080
     aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca      1140
     gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa      1200
     ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc      1260
     tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag      1320
     gaagagcaaa acaaaagtaa gaccaccgca cagcaagccc gctgatcttc agacctggag      1380
     gaggagatat gagggacatt ggagaagtga attatataaa tataaagtag taaaaattga      1440
     accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc      1500
     agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc      1560
     agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca      1620
     gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg      1680
     catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct      1740
     cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc      1800
     tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga      1860
     cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca      1920
     gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg      1980
     gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt      2040
     ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata      2100
     ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg      2160
     aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc      2220
     tcgacggtat cgaaagcttg ggattcgaat ttaaaagaaa aggggggatt ggggggtaca      2280
     gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa gaactacaaa      2340
     aacaaattac aaaaattcaa aattttcggg tttttcgaac ctaggtacgt ccaaggtcgg      2400
     gcaggaagag ggcctatttc ccatgattcc ttcatatttg catatacgat acaaggctgt      2460
     tagagagata attagaatta atttgactgt aaacacaaag atattagtac aaaatacgtg      2520
     acgtagaaag taataatttc ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga      2580
     ctatcatatg cttaccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg      2640
     gaaaggacaA AAANNNNNNN NNNNNNNNNN NNNNCGAANN NNNNNNNNNN NNNNNNNNNT      2700
     TTTTTGGTAC CAATTgtagt aatcaattac ggggtcatta gttcatagcc catatatgga      2760
     gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg      2820
     cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg      2880
     acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca      2940
     tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc      3000
     ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc      3060
     tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc      3120
     acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa      3180
     tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag      3240
     gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agggtaccgg      3300
     atccatggcc accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtcccccg      3360
     ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga      3420
     cccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg      3480
     gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac      3540
     gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggctcgcgca tggccgagtt      3600
     gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc      3660
     caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg      3720
     tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgctg gggtgcccgc      3780
     cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt      3840
     caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg      3900
     tgcctgaacg cgttccggaa atcaacctct ggattacaaa atttgtgaaa gattgactgg      3960
     tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta      4020
     tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct      4080
     gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt      4140
     tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac      4200
     tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg      4260
     ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac      4320
     gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg      4380
     ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct      4440
     gcggcctctt ccgcgtctcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc      4500
     tccccgcctg tccggatgga agggctaatt cactcccaac gaatacaaga tctgcttttt      4560
     gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta      4620
     gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc      4680
     cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa      4740
     atctctagca gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa      4800
     tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc      4860
     aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg      4920
     tccaaactca tcaatgtatc ttatcatgtc tggcatctat gtcgggtgcg gagaaagagg      4980
     taatgaaatg gcattatggg tattatgggt ctgcattaat gaatcggcca acggatgtcg      5040
     gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc      5100
     ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc      5160
     aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc      5220
     aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag      5280
     gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc      5340
     gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt      5400
     tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct      5460
     ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg      5520
     ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct      5580
     tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat      5640
     tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg      5700
     ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa      5760
     aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt      5820
     ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc      5880
     tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg ccatgagatt      5940
     atcaaaaagg atcttcacct agatcctttt cacgtagaaa gccagtccgc agaaacggtg      6000
     ctgaccccgg atgaatgtca gctactgggc tatctggaca agggaaaacg caagcgcaaa      6060
     gagaaagcag gtagcttgca gtgggcttac atggcgatag ctagactggg cggttttatg      6120
     gacagcaagc gaaccggaat tgccagctgg ggcgccctct ggtaaggttg ggaagccctg      6180
     caaagtaaac tggatggctt tcttgccgcc aaggatctga tggcgcaggg gatcaagctc      6240
     tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg      6300
     ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg      6360
     ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa      6420
     gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc tatcgtggct      6480
     ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga      6540
     ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc      6600
     cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac      6660
     ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc      6720
     cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact      6780
     gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga cccatggcga      6840
     tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg      6900
     ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga      6960
     agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga      7020
     ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgaattt tgttaaaatt      7080
     tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat      7140
     caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat      7200
     taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac      7260
     tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc      7320
     ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga      7380
     gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca      7440
     cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtccattc      7500
     gccattcagg atcgaattaa ttcttaatta acatcatcaa taatatacct t               7551