piLenti-shRNA
Vector Name | piLenti-shRNA |
VectorType | Lentiviral siRNA Vector |
Antibiotic Information | Bacterial: Kanamycin Mammalian: Puromycin |
Sequencing Primers | 5’--TACGTCCAAGGTCGGGCAGGAAGA--3’ in the U6 promoter region |
Additional Information | No Additional Information |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
Sequence 7551 BP; 1914 A; 1782 C; 2047 G; 1766 T; 42 other; ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt 60 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa 120 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg 180 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt 240 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg 300 tgttactcat agcgcgtaat actctcatga gcggatacat atttgaatgt atttagaaaa 360 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtaactataa 420 cggtcctaag gtagcgaaaa tgtagtctta tgcaatactc ttgtagtctt gcaacatggt 480 aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt 540 ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat 600 tggacgaacc actgaattgc cgcattgcag agatattgta tttaagtgcc tagctcgata 660 cataaacggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg 720 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg 780 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat 840 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct 900 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg 960 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc 1020 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga 1080 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca 1140 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa 1200 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc 1260 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag 1320 gaagagcaaa acaaaagtaa gaccaccgca cagcaagccc gctgatcttc agacctggag 1380 gaggagatat gagggacatt ggagaagtga attatataaa tataaagtag taaaaattga 1440 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc 1500 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc 1560 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca 1620 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg 1680 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct 1740 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc 1800 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga 1860 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca 1920 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg 1980 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt 2040 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata 2100 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg 2160 aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc 2220 tcgacggtat cgaaagcttg ggattcgaat ttaaaagaaa aggggggatt ggggggtaca 2280 gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa gaactacaaa 2340 aacaaattac aaaaattcaa aattttcggg tttttcgaac ctaggtacgt ccaaggtcgg 2400 gcaggaagag ggcctatttc ccatgattcc ttcatatttg catatacgat acaaggctgt 2460 tagagagata attagaatta atttgactgt aaacacaaag atattagtac aaaatacgtg 2520 acgtagaaag taataatttc ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga 2580 ctatcatatg cttaccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg 2640 gaaaggacaA AAANNNNNNN NNNNNNNNNN NNNNCGAANN NNNNNNNNNN NNNNNNNNNT 2700 TTTTTGGTAC CAATTgtagt aatcaattac ggggtcatta gttcatagcc catatatgga 2760 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg 2820 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg 2880 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca 2940 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc 3000 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc 3060 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc 3120 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa 3180 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag 3240 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agggtaccgg 3300 atccatggcc accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtcccccg 3360 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga 3420 cccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg 3480 gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac 3540 gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggctcgcgca tggccgagtt 3600 gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc 3660 caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg 3720 tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgctg gggtgcccgc 3780 cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt 3840 caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg 3900 tgcctgaacg cgttccggaa atcaacctct ggattacaaa atttgtgaaa gattgactgg 3960 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta 4020 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct 4080 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt 4140 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac 4200 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg 4260 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac 4320 gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg 4380 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct 4440 gcggcctctt ccgcgtctcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc 4500 tccccgcctg tccggatgga agggctaatt cactcccaac gaatacaaga tctgcttttt 4560 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta 4620 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc 4680 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa 4740 atctctagca gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa 4800 tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc 4860 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg 4920 tccaaactca tcaatgtatc ttatcatgtc tggcatctat gtcgggtgcg gagaaagagg 4980 taatgaaatg gcattatggg tattatgggt ctgcattaat gaatcggcca acggatgtcg 5040 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc 5100 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc 5160 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc 5220 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag 5280 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc 5340 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt 5400 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct 5460 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg 5520 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct 5580 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat 5640 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg 5700 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa 5760 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt 5820 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc 5880 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg ccatgagatt 5940 atcaaaaagg atcttcacct agatcctttt cacgtagaaa gccagtccgc agaaacggtg 6000 ctgaccccgg atgaatgtca gctactgggc tatctggaca agggaaaacg caagcgcaaa 6060 gagaaagcag gtagcttgca gtgggcttac atggcgatag ctagactggg cggttttatg 6120 gacagcaagc gaaccggaat tgccagctgg ggcgccctct ggtaaggttg ggaagccctg 6180 caaagtaaac tggatggctt tcttgccgcc aaggatctga tggcgcaggg gatcaagctc 6240 tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg 6300 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg 6360 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa 6420 gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc tatcgtggct 6480 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga 6540 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc 6600 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac 6660 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc 6720 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact 6780 gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga cccatggcga 6840 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg 6900 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga 6960 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga 7020 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgaattt tgttaaaatt 7080 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat 7140 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat 7200 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac 7260 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc 7320 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga 7380 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca 7440 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtccattc 7500 gccattcagg atcgaattaa ttcttaatta acatcatcaa taatatacct t 7551