pShuttle-His-18x
| Vector Name | pShuttle-His-18x |
| VectorType | Plasmid |
| Antibiotic Information | Kanamycin |
| Additional Information | No Additional Information |
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***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg
301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag
361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta
481 gcgaaagctc agatctggat ctcccgatcc cctatggtcg actctcagta caatctgctc
541 tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta
601 gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa
661 tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg
721 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
781 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
841 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
901 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
961 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
1021 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
1081 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
1141 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
1201 gaaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
1261 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctcccta tcagtgatag
1321 agatctccct atcagtgata gagatcgtcg acgagctcgt ttagtgaacc gtcagatcgc
1381 ctggagacgc catccacgct gttttgacct ccatagagct agcgtttaaa cttaagcttg
1441 gtaccgagct cggatccact agtccagtgt ggtggaattc tgcagatatc cagcacagtg
1501 gcgcggccgc aggcggcggc caccatcatc accatcacca ccaccatcac catcaccacc
1561 atcaccacca tcactgaatc tagagggccc gtttaaaccg ctgatcagcc tcgactgtgc
1621 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
1681 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
1741 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
1801 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca
1861 gcagatcgat ctctatgtcg ggtgcggaga aagaggtaat gaaatggcat tatgggtatt
1921 atgggtctgc attaatgaat cggccaagga tccatatgcg gtgtgaaata ccgcacagat
1981 gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc
2041 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
2101 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
2161 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
2221 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
2281 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
2341 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
2401 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
2461 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
2521 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
2581 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
2641 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
2701 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
2761 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
2821 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
2881 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
2941 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
3001 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
3061 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
3121 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
3181 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
3241 gtttgcgcaa cgttgttgaa aaaggatctt cacctagatc cttttcacgt agaaagccag
3301 tccgcagaaa cggtgctgac cccggatgaa tgtcagctac tgggctatct ggacaaggga
3361 aaacgcaagc gcaaagagaa agcaggtagc ttgcagtggg cttacatggc gatagctaga
3421 ctgggcggtt ttatggacag caagcgaacc ggaattgcca gctggggcgc cctctggtaa
3481 ggttgggaag ccctgcaaag taaactggat ggctttctcg ccgccaagga tctgatggcg
3541 caggggatca agctctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga
3601 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
3661 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
3721 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc
3781 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
3841 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
3901 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
3961 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
4021 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
4081 cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt
4141 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
4201 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
4261 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
4321 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
4381 aattttgtta aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
4441 acatccctta taaatcaaaa gaatagaccg cgatagggtt gagtgttgtt ccagtttgga
4501 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
4561 agggcgatgg cccactacgt gaaccatcac ccaaatcaag ttttttgcgg tcgaggtgcc
4621 gtaaagctct aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc
4681 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg
4741 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc gcgcttaatg cgccgctaca
4801 gggcgcgtcc attcgccatt caggatcgaa ttaattctta attaacatca tcaataatat
4861 acctt
LOCUS dna 4865 bp
FEATURES Location/Qualifiers
Other Gene 162..310
/gene="Encap other"
Promoter 365..393
/gene="amp prom"
misc_binding 457..462
/dbxref="REBASE:HpaI"
misc_binding 691..696
/dbxref="REBASE:NruI"
Regulatory_Seq 1305..1344
/gene="tetO reg"
misc_binding 1434..1439
/dbxref="REBASE:HindIII"
misc_binding 1440..1445
/dbxref="REBASE:KpnI"
misc_binding 1475..1480
/dbxref="REBASE:EcoRI"
misc_binding 1480..1485
/dbxref="REBASE:PstI"
misc_binding 1485..1490
/dbxref="REBASE:EcoRV"
misc_binding 1503..1510
/dbxref="REBASE:NotI"
misc_binding 1579..1584
/dbxref="REBASE:XbaI"
misc_binding 1585..1590
/dbxref="REBASE:ApaI"
misc_binding 1865..1870
/dbxref="REBASE:ClaI"
Rep_Origin 2177..2796
/gene="pBR322 origin"
Promoter 3449..3498
/gene="NEOKAN prom"
ORF 3587..4381
/sequence="ORF_1 rf(2)"
Marker 3590..4378
/gene="NTP_II marker"
Rep_Origin 4486..4791
/gene="f1 origin"
BASE COUNT 1191 a 1193 c 1320 g 1161 t 0 others
ORIGIN
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg
301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag
361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta
481 gcgaaagctc agatctggat ctcccgatcc cctatggtcg actctcagta caatctgctc
541 tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta
601 gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa
661 tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg
721 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
781 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
841 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
901 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
961 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
1021 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
1081 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
1141 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
1201 gaaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
1261 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctcccta tcagtgatag
1321 agatctccct atcagtgata gagatcgtcg acgagctcgt ttagtgaacc gtcagatcgc
1381 ctggagacgc catccacgct gttttgacct ccatagagct agcgtttaaa cttaagcttg
1441 gtaccgagct cggatccact agtccagtgt ggtggaattc tgcagatatc cagcacagtg
1501 gcgcggccgc aggcggcggc caccatcatc accatcacca ccaccatcac catcaccacc
1561 atcaccacca tcactgaatc tagagggccc gtttaaaccg ctgatcagcc tcgactgtgc
1621 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
1681 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
1741 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
1801 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca
1861 gcagatcgat ctctatgtcg ggtgcggaga aagaggtaat gaaatggcat tatgggtatt
1921 atgggtctgc attaatgaat cggccaagga tccatatgcg gtgtgaaata ccgcacagat
1981 gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc
2041 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
2101 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
2161 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
2221 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
2281 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
2341 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
2401 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
2461 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
2521 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
2581 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
2641 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
2701 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
2761 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
2821 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
2881 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
2941 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
3001 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
3061 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
3121 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
3181 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
3241 gtttgcgcaa cgttgttgaa aaaggatctt cacctagatc cttttcacgt agaaagccag
3301 tccgcagaaa cggtgctgac cccggatgaa tgtcagctac tgggctatct ggacaaggga
3361 aaacgcaagc gcaaagagaa agcaggtagc ttgcagtggg cttacatggc gatagctaga
3421 ctgggcggtt ttatggacag caagcgaacc ggaattgcca gctggggcgc cctctggtaa
3481 ggttgggaag ccctgcaaag taaactggat ggctttctcg ccgccaagga tctgatggcg
3541 caggggatca agctctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga
3601 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
3661 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
3721 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc
3781 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
3841 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
3901 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
3961 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
4021 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
4081 cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt
4141 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
4201 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
4261 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
4321 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
4381 aattttgtta aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
4441 acatccctta taaatcaaaa gaatagaccg cgatagggtt gagtgttgtt ccagtttgga
4501 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
4561 agggcgatgg cccactacgt gaaccatcac ccaaatcaag ttttttgcgg tcgaggtgcc
4621 gtaaagctct aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc
4681 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg
4741 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc gcgcttaatg cgccgctaca
4801 gggcgcgtcc attcgccatt caggatcgaa ttaattctta attaacatca tcaataatat
4861 acctt
//
***Note: The text map shown below is representative of the parental
vector only and does not include the gene of interest. The sequence of the gene
can be found through the accession number link on the product page.
1 ttttggattgaagccaatatgataatgagggggtggagtttgtgacgtggcgcggggcgt 60
AAAACCTAACTTCGGTTATACTATTACTCCCCCACCTCAAACACTGCACCGCGCCCCGCA
61 gggaacggggcgggtgacgtagtagtgtggcggaagtgtgatgttgcaagtgtggcggaa 120
CCCTTGCCCCGCCCACTGCATCATCACACCGCCTTCACACTACAACGTTCACACCGCCTT
Encap other(162,310)>>>
|
121 cacatgtaagcgacggatgtggcaaaagtgacgtttttggtgtgcgccggtgtacacagg 180
GTGTACATTCGCTGCCTACACCGTTTTCACTGCAAAAACCACACGCGGCCACATGTGTCC
181 aagtgacaattttcgcgcggttttaggcggatgttgtagtaaatttgggcgtaaccgagt 240
TTCACTGTTAAAAGCGCGCCAAAATCCGCCTACAACATCATTTAAACCCGCATTGGCTCA
241 aagatttggccattttcgcgggaaaactgaataagaggaagtgaaatctgaataattttg 300
TTCTAAACCGGTAAAAGCGCCCTTTTGACTTATTCTCCTTCACTTTAGACTTATTAAAAC
301 tgttactcatagcgcgtaatacggcagacctcagcgctagattattgaagcatttatcag 360
ACAATGAGTATCGCGCATTATGCCGTCTGGAGTCGCGATCTAATAACTTCGTAAATAGTC
amp prom(365,393)<<<
|
361 ggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggg 420
CCAATAACAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTATCCC
HpaI
|
421 gttccgcgcacatttccccgaaaagtgccacctgacgttaactataacggtcctaaggta 480
CAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTGCAATTGATATTGCCAGGATTCCAT
481 gcgaaagctcagatctggatctcccgatcccctatggtcgactctcagtacaatctgctc 540
CGCTTTCGAGTCTAGACCTAGAGGGCTAGGGGATACCAGCTGAGAGTCATGTTAGACGAG
541 tgatgccgcatagttaagccagtatctgctccctgcttgtgtgttggaggtcgctgagta 600
ACTACGGCGTATCAATTCGGTCATAGACGAGGGACGAACACACAACCTCCAGCGACTCAT
601 gtgcgcgagcaaaatttaagctacaacaaggcaaggcttgaccgacaattgcatgaagaa 660
CACGCGCTCGTTTTAAATTCGATGTTGTTCCGTTCCGAACTGGCTGTTAACGTACTTCTT
NruI
|
661 tctgcttagggttaggcgttttgcgctgcttcgcgatgtacgggccagatatacgcgttg 720
AGACGAATCCCAATCCGCAAAACGCGACGAAGCGCTACATGCCCGGTCTATATGCGCAAC
721 acattgattattgactagttattaatagtaatcaattacggggtcattagttcatagccc 780
TGTAACTAATAACTGATCAATAATTATCATTAGTTAATGCCCCAGTAATCAAGTATCGGG
781 atatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaa 840
TATATACCTCAAGGCGCAATGTATTGAATGCCATTTACCGGGCGGACCGACTGGCGGGTT
841 cgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggac 900
GCTGGGGGCGGGTAACTGCAGTTATTACTGCATACAAGGGTATCATTGCGGTTATCCCTG
901 tttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatca 960
AAAGGTAACTGCAGTTACCCACCTCATAAATGCCATTTGACGGGTGAACCGTCATGTAGT
961 agtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctg 1020
TCACATAGTATACGGTTCATGCGGGGGATAACTGCAGTTACTGCCATTTACCGGGCGGAC
1021 gcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtatt 1080
CGTAATACGGGTCATGTACTGGAATACCCTGAAAGGATGAACCGTCATGTAGATGCATAA
1081 agtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcg 1140
TCAGTAGCGATAATGGTACCACTACGCCAAAACCGTCATGTAGTTACCCGCACCTATCGC
1141 gtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttg 1200
CAAACTGAGTGCCCCTAAAGGTTCAGAGGTGGGGTAACTGCAGTTACCCTCAAACAAAAC
1201 gaaccaaaatcaacgggactttccaaaatgtcgtaacaactccgccccattgacgcaaat 1260
CTTGGTTTTAGTTGCCCTGAAAGGTTTTACAGCATTGTTGAGGCGGGGTAACTGCGTTTA
tetO reg(1305,1344)>>>
|
1261 gggcggtaggcgtgtacggtgggaggtctatataagcagagctctccctatcagtgatag 1320
CCCGCCATCCGCACATGCCACCCTCCAGATATATTCGTCTCGAGAGGGATAGTCACTATC
1321 agatctccctatcagtgatagagatcgtcgacgagctcgtttagtgaaccgtcagatcgc 1380
TCTAGAGGGATAGTCACTATCTCTAGCAGCTGCTCGAGCAAATCACTTGGCAGTCTAGCG
HindIII
|
1381 ctggagacgccatccacgctgttttgacctccatagagctagcgtttaaacttaagcttg 1440
GACCTCTGCGGTAGGTGCGACAAAACTGGAGGTATCTCGATCGCAAATTTGAATTCGAAC
EcoRV
|
KpnI EcoRI PstI
| | | |
1441 gtaccgagctcggatccactagtccagtgtggtggaattctgcagatatccagcacagtg 1500
CATGGCTCGAGCCTAGGTGATCAGGTCACACCACCTTAAGACGTCTATAGGTCGTGTCAC
NotI
|
1501 gcgcggccgcaggcggcggcCACCATCATCACCATCACCACCACCATCACCATCACCACC 1560
CGCGCCGGCGTCCGCCGCCGGTGGTAGTAGTGGTAGTGGTGGTGGTAGTGGTAGTGGTGG
XbaI ApaI
| |
1561 ATCACCACCATCACTGAAtctagagggcccgtttaaaccgctgatcagcctcgactgtgc 1620
TAGTGGTGGTAGTGACTTAGATCTCCCGGGCAAATTTGGCGACTAGTCGGAGCTGACACG
1621 cttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaag 1680
GAAGATCAACGGTCGGTAGACAACAAACGGGGAGGGGGCACGGAAGGAACTGGGACCTTC
1681 gtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagta 1740
CACGGTGAGGGTGACAGGAAAGGATTATTTTACTCCTTTAACGTAGCGTAACAGACTCAT
1741 ggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaag 1800
CCACAGTAAGATAAGACCCCCCACCCCACCCCGTCCTGTCGTTCCCCCTCCTAACCCTTC
1801 acaatagcaggcatgctggggatgcggtgggctctatggcttctgaggcggaaagaacca 1860
TGTTATCGTCCGTACGACCCCTACGCCACCCGAGATACCGAAGACTCCGCCTTTCTTGGT
ClaI
|
1861 gcagatcgatctctatgtcgggtgcggagaaagaggtaatgaaatggcattatgggtatt 1920
CGTCTAGCTAGAGATACAGCCCACGCCTCTTTCTCCATTACTTTACCGTAATACCCATAA
1921 atgggtctgcattaatgaatcggccaaggatccatatgcggtgtgaaataccgcacagat 1980
TACCCAGACGTAATTACTTAGCCGGTTCCTAGGTATACGCCACACTTTATGGCGTGTCTA
1981 gcgtaaggagaaaataccgcatcaggcgctcttccgcttcctcgctcactgactcgctgc 2040
CGCATTCCTCTTTTATGGCGTAGTCCGCGAGAAGGCGAAGGAGCGAGTGACTGAGCGACG
2041 gctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttat 2100
CGAGCCAGCAAGCCGACGCCGCTCGCCATAGTCGAGTGAGTTTCCGCCATTATGCCAATA
2101 ccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggcca 2160
GGTGTCTTAGTCCCCTATTGCGTCCTTTCTTGTACACTCGTTTTCCGGTCGTTTTCCGGT
pBR322 origin(2177,2796)<<<
|
2161 ggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagc 2220
CCTTGGCATTTTTCCGGCGCAACGACCGCAAAAAGGTATCCGAGGCGGGGGGACTGCTCG
2221 atcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagatacc 2280
TAGTGTTTTTAGCTGCGAGTTCAGTCTCCACCGCTTTGGGCTGTCCTGATATTTCTATGG
2281 aggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccg 2340
TCCGCAAAGGGGGACCTTCGAGGGAGCACGCGAGAGGACAAGGCTGGGACGGCGAATGGC
2341 gatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgta 2400
CTATGGACAGGCGGAAAGAGGGAAGCCCTTCGCACCGCGAAAGAGTATCGAGTGCGACAT
2401 ggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccg 2460
CCATAGAGTCAAGCCACATCCAGCAAGCGAGGTTCGACCCGACACACGTGCTTGGGGGGC
2461 ttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagac 2520
AAGTCGGGCTGGCGACGCGGAATAGGCCATTGATAGCAGAACTCAGGTTGGGCCATTCTG
2521 acgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtag 2580
TGCTGAATAGCGGTGACCGTCGTCGGTGACCATTGTCCTAATCGTCTCGCTCCATACATC
2581 gcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtat 2640
CGCCACGATGTCTCAAGAACTTCACCACCGGATTGATGCCGATGTGATCTTCCTGTCATA
2641 ttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgat 2700
AACCATAGACGCGAGACGACTTCGGTCAATGGAAGCCTTTTTCTCAACCATCGAGAACTA
2701 ccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgc 2760
GGCCGTTTGTTTGGTGGCGACCATCGCCACCAAAAAAACAAACGTTCGTCGTCTAATGCG
2761 gcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagt 2820
CGTCTTTTTTTCCTAGAGTTCTTCTAGGAAACTAGAAAAGATGCCCCAGACTGCGAGTCA
2821 ggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacct 2880
CCTTGCTTTTGAGTGCAATTCCCTAAAACCAGTACTCTAATAGTTTTTCCTAGAAGTGGA
2881 agatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaactt 2940
TCTAGGAAAATTTAATTTTTACTTCAAAATTTAGTTAGATTTCATATATACTCATTTGAA
2941 ggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttc 3000
CCAGACTGTCAATGGTTACGAATTAGTCACTCCGTGGATAGAGTCGCTAGACAGATAAAG
3001 gttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttac 3060
CAAGTAGGTATCAACGGACTGAGGGGCAGCACATCTATTGATGCTATGCCCTCCCGAATG
3061 catctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttat 3120
GTAGACCGGGGTCACGACGTTACTATGGCGCTCTGGGTGCGAGTGGCCGAGGTCTAAATA
3121 cagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccg 3180
GTCGTTATTTGGTCGGTCGGCCTTCCCGGCTCGCGTCTTCACCAGGACGTTGAAATAGGC
3181 cctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaata 3240
GGAGGTAGGTCAGATAATTAACAACGGCCCTTCGATCTCATTCATCAAGCGGTCAATTAT
3241 gtttgcgcaacgttgttgaaaaaggatcttcacctagatccttttcacgtagaaagccag 3300
CAAACGCGTTGCAACAACTTTTTCCTAGAAGTGGATCTAGGAAAAGTGCATCTTTCGGTC
3301 tccgcagaaacggtgctgaccccggatgaatgtcagctactgggctatctggacaaggga 3360
AGGCGTCTTTGCCACGACTGGGGCCTACTTACAGTCGATGACCCGATAGACCTGTTCCCT
3361 aaacgcaagcgcaaagagaaagcaggtagcttgcagtgggcttacatggcgatagctaga 3420
TTTGCGTTCGCGTTTCTCTTTCGTCCATCGAACGTCACCCGAATGTACCGCTATCGATCT
NEOKAN prom(3449,3498)>>>
|
3421 ctgggcggttttatggacagcaagcgaaccggaattgccagctggggcgccctctggtaa 3480
GACCCGCCAAAATACCTGTCGTTCGCTTGGCCTTAACGGTCGACCCCGCGGGAGACCATT
3481 ggttgggaagccctgcaaagtaaactggatggctttctcgccgccaaggatctgatggcg 3540
CCAACCCTTCGGGACGTTTCATTTGACCTACCGAAAGAGCGGCGGTTCCTAGACTACCGC
NTP_II marker(3590,4378)>>>
|
ORF_1 rf(2)(3587,4381)>>>
| |
3541 caggggatcaagctctgatcaagagacaggatgaggatcgtttcgcatgattgaacaaga 3600
GTCCCCTAGTTCGAGACTAGTTCTCTGTCCTACTCCTAGCAAAGCGTACTAACTTGTTCT
3601 tggattgcacgcaggttctccggccgcttgggtggagaggctattcggctatgactgggc 3660
ACCTAACGTGCGTCCAAGAGGCCGGCGAACCCACCTCTCCGATAAGCCGATACTGACCCG
3661 acaacagacaatcggctgctctgatgccgccgtgttccggctgtcagcgcaggggcgccc 3720
TGTTGTCTGTTAGCCGACGAGACTACGGCGGCACAAGGCCGACAGTCGCGTCCCCGCGGG
3721 ggttctttttgtcaagaccgacctgtccggtgccctgaatgaactgcaagacgaggcagc 3780
CCAAGAAAAACAGTTCTGGCTGGACAGGCCACGGGACTTACTTGACGTTCTGCTCCGTCG
3781 gcggctatcgtggctggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcac 3840
CGCCGATAGCACCGACCGGTGCTGCCCGCAAGGAACGCGTCGACACGAGCTGCAACAGTG
3841 tgaagcgggaagggactggctgctattgggcgaagtgccggggcaggatctcctgtcatc 3900
ACTTCGCCCTTCCCTGACCGACGATAACCCGCTTCACGGCCCCGTCCTAGAGGACAGTAG
3901 tcaccttgctcctgccgagaaagtatccatcatggctgatgcaatgcggcggctgcatac 3960
AGTGGAACGAGGACGGCTCTTTCATAGGTAGTACCGACTACGTTACGCCGCCGACGTATG
3961 gcttgatccggctacctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacg 4020
CGAACTAGGCCGATGGACGGGTAAGCTGGTGGTTCGCTTTGTAGCGTAGCTCGCTCGTGC
4021 tactcggatggaagccggtcttgtcgatcaggatgatctggacgaagagcatcaggggct 4080
ATGAGCCTACCTTCGGCCAGAACAGCTAGTCCTACTAGACCTGCTTCTCGTAGTCCCCGA
4081 cgcgccagccgaactgttcgccaggctcaaggcgagcatgcccgacggcgaggatctcgt 4140
GCGCGGTCGGCTTGACAAGCGGTCCGAGTTCCGCTCGTACGGGCTGCCGCTCCTAGAGCA
4141 cgtgacccatggcgatgcctgcttgccgaatatcatggtggaaaatggccgcttttctgg 4200
GCACTGGGTACCGCTACGGACGAACGGCTTATAGTACCACCTTTTACCGGCGAAAAGACC
4201 attcatcgactgtggccggctgggtgtggcggaccgctatcaggacatagcgttggctac 4260
TAAGTAGCTGACACCGGCCGACCCACACCGCCTGGCGATAGTCCTGTATCGCAACCGATG
4261 ccgtgatattgctgaagagcttggcggcgaatgggctgaccgcttcctcgtgctttacgg 4320
GGCACTATAACGACTTCTCGAACCGCCGCTTACCCGACTGGCGAAGGAGCACGAAATGCC
4321 tatcgccgctcccgattcgcagcgcatcgccttctatcgccttcttgacgagttcttctg 4380
ATAGCGGCGAGGGCTAAGCGTCGCGTAGCGGAAGATAGCGGAAGAACTGCTCAAGAAGAC
4381 aattttgttaaaatttttgttaaatcagctcattttttaaccaataggccgaaatcggca 4440
TTAAAACAATTTTAAAAACAATTTAGTCGAGTAAAAAATTGGTTATCCGGCTTTAGCCGT
f1 origin(4486,4791)<<<
|
4441 acatcccttataaatcaaaagaatagaccgcgatagggttgagtgttgttccagtttgga 4500
TGTAGGGAATATTTAGTTTTCTTATCTGGCGCTATCCCAACTCACAACAAGGTCAAACCT
4501 acaagagtccactattaaagaacgtggactccaacgtcaaagggcgaaaaaccgtctatc 4560
TGTTCTCAGGTGATAATTTCTTGCACCTGAGGTTGCAGTTTCCCGCTTTTTGGCAGATAG
4561 agggcgatggcccactacgtgaaccatcacccaaatcaagttttttgcggtcgaggtgcc 4620
TCCCGCTACCGGGTGATGCACTTGGTAGTGGGTTTAGTTCAAAAAACGCCAGCTCCACGG
4621 gtaaagctctaaatcggaaccctaaagggagcccccgatttagagcttgacggggaaagc 4680
CATTTCGAGATTTAGCCTTGGGATTTCCCTCGGGGGCTAAATCTCGAACTGCCCCTTTCG
4681 cggcgaacgtggcgagaaaggaagggaagaaagcgaaaggagcgggcgctagggcgctgg 4740
GCCGCTTGCACCGCTCTTTCCTTCCCTTCTTTCGCTTTCCTCGCCCGCGATCCCGCGACC
4741 caagtgtagcggtcacgctgcgcgtaaccaccacacccgcgcgcttaatgcgccgctaca 4800
GTTCACATCGCCAGTGCGACGCGCATTGGTGGTGTGGGCGCGCGAATTACGCGGCGATGT
4801 gggcgcgtccattcgccattcaggatcgaattaattcttaattaacatcatcaataatat 4860
CCCGCGCAGGTAAGCGGTAAGTCCTAGCTTAATTAAGAATTAATTGTAGTAGTTATTATA
4861 acctt 4865
TGGAA
LOCUS dna 4865 bp
FEATURES Location/Qualifiers
Other Gene 162..310
/gene="Encap other"
Promoter 365..393
/gene="amp prom"
misc_binding 457..462
/dbxref="REBASE:HpaI"
misc_binding 691..696
/dbxref="REBASE:NruI"
Regulatory_Seq 1305..1344
/gene="tetO reg"
misc_binding 1434..1439
/dbxref="REBASE:HindIII"
misc_binding 1440..1445
/dbxref="REBASE:KpnI"
misc_binding 1475..1480
/dbxref="REBASE:EcoRI"
misc_binding 1480..1485
/dbxref="REBASE:PstI"
misc_binding 1485..1490
/dbxref="REBASE:EcoRV"
misc_binding 1503..1510
/dbxref="REBASE:NotI"
misc_binding 1579..1584
/dbxref="REBASE:XbaI"
misc_binding 1585..1590
/dbxref="REBASE:ApaI"
misc_binding 1865..1870
/dbxref="REBASE:ClaI"
Rep_Origin 2177..2796
/gene="pBR322 origin"
Promoter 3449..3498
/gene="NEOKAN prom"
ORF 3587..4381
/sequence="ORF_1 rf(2)"
Marker 3590..4378
/gene="NTP_II marker"
Rep_Origin 4486..4791
/gene="f1 origin"
BASE COUNT 1191 a 1193 c 1320 g 1161 t 0 others