pShuttle-2A-RFP
| Vector Name | pShuttle-2A-RFP |
| VectorType | Plasmid |
| Antibiotic Information | Kanamycin |
| Additional Information | No Additional Information |
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***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg
301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag
361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta
481 gcgaaagctc agatctggat ctcccgatcc cctatggtcg actctcagta caatctgctc
541 tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta
601 gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa
661 tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg
721 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
781 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
841 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
901 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
961 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
1021 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
1081 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
1141 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
1201 gaaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
1261 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctcccta tcagtgatag
1321 agatctccct atcagtgata gagatcgtcg acgagctcgt ttagtgaacc gtcagatcgc
1381 ctggagacgc catccacgct gttttgacct ccatagaagc tagccttaag aagcttggta
1441 ccgaattcga tatcctgcag agcggccgct ctagcttaga gggcagagga agtcttctaa
1501 catgcggtga cgtggaggag aatcccggcc cttctagaat gagcgagctg atcaaggaga
1561 acatgcacat gaagctgtac atggagggca ccgtgaacaa ccaccacttc aagtgcacat
1621 ccgagggcga aggcaagccc tacgagggca cccagaccat gaagatcaag gtggtcgagg
1681 gcggccctct ccccttcgcc ttcgacatcc tggctaccag cttcatgtac ggcagcaaag
1741 ccttcatcaa ccacacccag ggcatccccg acttctttaa gcagtccttc cctgagggct
1801 tcacatggga gagaatcacc acatacgaag acgggggcgt gctgaccgct acccaggaca
1861 ccagcttcca gaacggctgc atcatctaca acgtcaagat caacggggtg aacttcccat
1921 ccaacggccc tgtgatgcag aagaaaacac gcggctggga ggccaacacc gagatgctgt
1981 accccgctga cggcggcctg agaggccaca gccagatggc cctgaagctc gtgggcgggg
2041 gctacctgca ctgctccttc aagaccacat acagatccaa gaaacccgct aagaacctca
2101 agatgcccgg cttccacttc gtggaccaca gactggaaag aatcaaggag gccgacaaag
2161 agacctacgt cgagcagcac gagatggctg tggccaagta ctgcgacctc cctagcaaac
2221 tggggcacag agggtctaga tgactcgagt acccatacga cgtcccagac tacgcttgag
2281 tttaaaccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgttgcccg
2341 ggcgcgatcg ctgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
2401 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
2461 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
2521 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagcaga tcgatctgca
2581 tctatgtcgg gtgcggagaa agaggtaatg aaatggcatt atgggtatta tgggtctgca
2641 ttaatgaatc ggccaacgga tcccggtgtg aaataccgca cagatgcgta aggagaaaat
2701 accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
2761 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
2821 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
2881 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
2941 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
3001 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
3061 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
3121 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
3181 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
3241 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
3301 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
3361 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
3421 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
3481 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
3541 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
3601 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
3661 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
3721 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
3781 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
3841 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
3901 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
3961 ttgaaaaagg atcttcacct agatcctttt cacgtagaaa gccagtccgc agaaacggtg
4021 ctgaccccgg atgaatgtca gctactgggc tatctggaca agggaaaacg caagcgcaaa
4081 gagaaagcag gtagcttgca gtgggcttac atggcgatag ctagactggg cggttttatg
4141 gacagcaagc gaaccggaat tgccagctgg ggcgccctct ggtaaggttg ggaagccctg
4201 caaagtaaac tggatggctt tctcgccgcc aaggatctga tggcgcaggg gatcaagctc
4261 tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg
4321 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
4381 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
4441 gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc tatcgtggct
4501 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
4561 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
4621 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
4681 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
4741 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact
4801 gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga cccatggcga
4861 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
4921 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
4981 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
5041 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgaattt tgttaaaatt
5101 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaacatc ccttataaat
5161 caaaagaata gaccgcgata gggttgagtg ttgttccagt ttggaacaag agtccactat
5221 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac
5281 tacgtgaacc atcacccaaa tcaagttttt tgcggtcgag gtgccgtaaa gctctaaatc
5341 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga
5401 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
5461 cgctgcgcgt aaccaccaca cccgcgcgct taatgcgccg ctacagggcg cgtccattcg
5521 ccattcagga tcgaattaat tcttaattaa catcatcaat aatatacctt
LOCUS dna 5570 bp
FEATURES Location/Qualifiers
Other Gene 162..310
/gene="Encap other"
Promoter 365..393
/gene="amp prom"
misc_binding 457..462
/dbxref="REBASE:HpaI"
misc_binding 691..696
/dbxref="REBASE:NruI"
misc_binding 968..973
/dbxref="REBASE:NdeI"
Regulatory_Seq 1305..1344
/gene="tetO reg"
misc_binding 1431..1436
/dbxref="REBASE:HindIII"
misc_binding 1437..1442
/dbxref="REBASE:KpnI"
misc_binding 1443..1448
/dbxref="REBASE:EcoRI"
misc_binding 1449..1454
/dbxref="REBASE:EcoRV"
misc_binding 1455..1460
/dbxref="REBASE:PstI"
misc_binding 1462..1469
/dbxref="REBASE:NotI"
ORF 1539..2243
/sequence="ORF_2 rf(3)"
misc_binding 1995..2007
/dbxref="REBASE:SfiI"
misc_binding 2244..2249
/dbxref="REBASE:XhoI"
misc_binding 2337..2342
/dbxref="REBASE:XmaI"
misc_binding 2337..2342
/dbxref="REBASE:SmaI"
misc_binding 2570..2575
/dbxref="REBASE:ClaI"
misc_binding 2658..2663
/dbxref="REBASE:BamHI"
Rep_Origin 2882..3501
/gene="pBR322 origin"
Promoter 4154..4203
/gene="NEOKAN prom"
ORF 4292..5086
/sequence="ORF_1 rf(2)"
Marker 4295..5083
/gene="NTP_II marker"
Rep_Origin 5191..5496
/gene="f1 origin"
***Note: The text map shown below is representative of the parental
vector only and does not include the gene of interest. The sequence of the gene
can be found through the accession number link on the product page.
1 ttttggattgaagccaatatgataatgagggggtggagtttgtgacgtggcgcggggcgt 60
AAAACCTAACTTCGGTTATACTATTACTCCCCCACCTCAAACACTGCACCGCGCCCCGCA
61 gggaacggggcgggtgacgtagtagtgtggcggaagtgtgatgttgcaagtgtggcggaa 120
CCCTTGCCCCGCCCACTGCATCATCACACCGCCTTCACACTACAACGTTCACACCGCCTT
Encap other(162,310)>>>
|
121 cacatgtaagcgacggatgtggcaaaagtgacgtttttggtgtgcgccggtgtacacagg 180
GTGTACATTCGCTGCCTACACCGTTTTCACTGCAAAAACCACACGCGGCCACATGTGTCC
181 aagtgacaattttcgcgcggttttaggcggatgttgtagtaaatttgggcgtaaccgagt 240
TTCACTGTTAAAAGCGCGCCAAAATCCGCCTACAACATCATTTAAACCCGCATTGGCTCA
241 aagatttggccattttcgcgggaaaactgaataagaggaagtgaaatctgaataattttg 300
TTCTAAACCGGTAAAAGCGCCCTTTTGACTTATTCTCCTTCACTTTAGACTTATTAAAAC
301 tgttactcatagcgcgtaatacggcagacctcagcgctagattattgaagcatttatcag 360
ACAATGAGTATCGCGCATTATGCCGTCTGGAGTCGCGATCTAATAACTTCGTAAATAGTC
amp prom(365,393)<<<
|
361 ggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggg 420
CCAATAACAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTATCCC
HpaI
|
421 gttccgcgcacatttccccgaaaagtgccacctgacgttaactataacggtcctaaggta 480
CAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTGCAATTGATATTGCCAGGATTCCAT
481 gcgaaagctcagatctggatctcccgatcccctatggtcgactctcagtacaatctgctc 540
CGCTTTCGAGTCTAGACCTAGAGGGCTAGGGGATACCAGCTGAGAGTCATGTTAGACGAG
541 tgatgccgcatagttaagccagtatctgctccctgcttgtgtgttggaggtcgctgagta 600
ACTACGGCGTATCAATTCGGTCATAGACGAGGGACGAACACACAACCTCCAGCGACTCAT
601 gtgcgcgagcaaaatttaagctacaacaaggcaaggcttgaccgacaattgcatgaagaa 660
CACGCGCTCGTTTTAAATTCGATGTTGTTCCGTTCCGAACTGGCTGTTAACGTACTTCTT
NruI
|
661 tctgcttagggttaggcgttttgcgctgcttcgcgatgtacgggccagatatacgcgttg 720
AGACGAATCCCAATCCGCAAAACGCGACGAAGCGCTACATGCCCGGTCTATATGCGCAAC
721 acattgattattgactagttattaatagtaatcaattacggggtcattagttcatagccc 780
TGTAACTAATAACTGATCAATAATTATCATTAGTTAATGCCCCAGTAATCAAGTATCGGG
781 atatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaa 840
TATATACCTCAAGGCGCAATGTATTGAATGCCATTTACCGGGCGGACCGACTGGCGGGTT
841 cgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggac 900
GCTGGGGGCGGGTAACTGCAGTTATTACTGCATACAAGGGTATCATTGCGGTTATCCCTG
901 tttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatca 960
AAAGGTAACTGCAGTTACCCACCTCATAAATGCCATTTGACGGGTGAACCGTCATGTAGT
NdeI
|
961 agtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctg 1020
TCACATAGTATACGGTTCATGCGGGGGATAACTGCAGTTACTGCCATTTACCGGGCGGAC
1021 gcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtatt 1080
CGTAATACGGGTCATGTACTGGAATACCCTGAAAGGATGAACCGTCATGTAGATGCATAA
1081 agtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcg 1140
TCAGTAGCGATAATGGTACCACTACGCCAAAACCGTCATGTAGTTACCCGCACCTATCGC
1141 gtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttg 1200
CAAACTGAGTGCCCCTAAAGGTTCAGAGGTGGGGTAACTGCAGTTACCCTCAAACAAAAC
1201 gaaccaaaatcaacgggactttccaaaatgtcgtaacaactccgccccattgacgcaaat 1260
CTTGGTTTTAGTTGCCCTGAAAGGTTTTACAGCATTGTTGAGGCGGGGTAACTGCGTTTA
tetO reg(1305,1344)>>>
|
1261 gggcggtaggcgtgtacggtgggaggtctatataagcagagctctccctatcagtgatag 1320
CCCGCCATCCGCACATGCCACCCTCCAGATATATTCGTCTCGAGAGGGATAGTCACTATC
1321 agatctccctatcagtgatagagatcgtcgacgagctcgtttagtgaaccgtcagatcgc 1380
TCTAGAGGGATAGTCACTATCTCTAGCAGCTGCTCGAGCAAATCACTTGGCAGTCTAGCG
HindIII
|
1381 ctggagacgccatccacgctgttttgacctccatagaagctagccttaagaagcttggta 1440
GACCTCTGCGGTAGGTGCGACAAAACTGGAGGTATCTTCGATCGGAATTCTTCGAACCAT
EcoRI NotI
| |
KpnI EcoRV PstI
| | | | |
1441 ccgaattcgatatcctgcagagcggccgctctagcttagagggcagaggaagtcttctaa 1500
GGCTTAAGCTATAGGACGTCTCGCCGGCGAGATCGAATCTCCCGTCTCCTTCAGAAGATT
ORF_2 rf(3)(1539,2243)>>>
|
1501 catgcggtgacgtggaggagaatcccggcccttctagaatgagcgagctgatcaaggaga 1560
GTACGCCACTGCACCTCCTCTTAGGGCCGGGAAGATCTTACTCGCTCGACTAGTTCCTCT
1561 acatgcacatgaagctgtacatggagggcaccgtgaacaaccaccacttcaagtgcacat 1620
TGTACGTGTACTTCGACATGTACCTCCCGTGGCACTTGTTGGTGGTGAAGTTCACGTGTA
1621 ccgagggcgaaggcaagccctacgagggcacccagaccatgaagatcaaggtggtcgagg 1680
GGCTCCCGCTTCCGTTCGGGATGCTCCCGTGGGTCTGGTACTTCTAGTTCCACCAGCTCC
1681 gcggccctctccccttcgccttcgacatcctggctaccagcttcatgtacggcagcaaag 1740
CGCCGGGAGAGGGGAAGCGGAAGCTGTAGGACCGATGGTCGAAGTACATGCCGTCGTTTC
1741 ccttcatcaaccacacccagggcatccccgacttctttaagcagtccttccctgagggct 1800
GGAAGTAGTTGGTGTGGGTCCCGTAGGGGCTGAAGAAATTCGTCAGGAAGGGACTCCCGA
1801 tcacatgggagagaatcaccacatacgaagacgggggcgtgctgaccgctacccaggaca 1860
AGTGTACCCTCTCTTAGTGGTGTATGCTTCTGCCCCCGCACGACTGGCGATGGGTCCTGT
1861 ccagcttccagaacggctgcatcatctacaacgtcaagatcaacggggtgaacttcccat 1920
GGTCGAAGGTCTTGCCGACGTAGTAGATGTTGCAGTTCTAGTTGCCCCACTTGAAGGGTA
1921 ccaacggccctgtgatgcagaagaaaacacgcggctgggaggccaacaccgagatgctgt 1980
GGTTGCCGGGACACTACGTCTTCTTTTGTGCGCCGACCCTCCGGTTGTGGCTCTACGACA
SfiI
|
1981 accccgctgacggcggcctgagaggccacagccagatggccctgaagctcgtgggcgggg 2040
TGGGGCGACTGCCGCCGGACTCTCCGGTGTCGGTCTACCGGGACTTCGAGCACCCGCCCC
2041 gctacctgcactgctccttcaagaccacatacagatccaagaaacccgctaagaacctca 2100
CGATGGACGTGACGAGGAAGTTCTGGTGTATGTCTAGGTTCTTTGGGCGATTCTTGGAGT
2101 agatgcccggcttccacttcgtggaccacagactggaaagaatcaaggaggccgacaaag 2160
TCTACGGGCCGAAGGTGAAGCACCTGGTGTCTGACCTTTCTTAGTTCCTCCGGCTGTTTC
2161 agacctacgtcgagcagcacgagatggctgtggccaagtactgcgacctccctagcaaac 2220
TCTGGATGCAGCTCGTCGTGCTCTACCGACACCGGTTCATGACGCTGGAGGGATCGTTTG
XhoI
|
2221 tggggcacagagggtctagatgactcgagtacccatacgacgtcccagactacgcttgag 2280
ACCCCGTGTCTCCCAGATCTACTGAGCTCATGGGTATGCTGCAGGGTCTGATGCGAACTC
XmaI
|
2281 tttaaaccgctgatcagcctcgactgtgccttctagttgccagccatctgttgttgcccg 2340
AAATTTGGCGACTAGTCGGAGCTGACACGGAAGATCAACGGTCGGTAGACAACAACGGGC
SmaI
|
2341 ggcgcgatcgctgcccctcccccgtgccttccttgaccctggaaggtgccactcccactg 2400
CCGCGCTAGCGACGGGGAGGGGGCACGGAAGGAACTGGGACCTTCCACGGTGAGGGTGAC
2401 tcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattc 2460
AGGAAAGGATTATTTTACTCCTTTAACGTAGCGTAACAGACTCATCCACAGTAAGATAAG
2461 tggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatg 2520
ACCCCCCACCCCACCCCGTCCTGTCGTTCCCCCTCCTAACCCTTCTGTTATCGTCCGTAC
ClaI
|
2521 ctggggatgcggtgggctctatggcttctgaggcggaaagaaccagcagatcgatctgca 2580
GACCCCTACGCCACCCGAGATACCGAAGACTCCGCCTTTCTTGGTCGTCTAGCTAGACGT
2581 tctatgtcgggtgcggagaaagaggtaatgaaatggcattatgggtattatgggtctgca 2640
AGATACAGCCCACGCCTCTTTCTCCATTACTTTACCGTAATACCCATAATACCCAGACGT
BamHI
|
2641 ttaatgaatcggccaacggatcccggtgtgaaataccgcacagatgcgtaaggagaaaat 2700
AATTACTTAGCCGGTTGCCTAGGGCCACACTTTATGGCGTGTCTACGCATTCCTCTTTTA
2701 accgcatcaggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggc 2760
TGGCGTAGTCCGCGAGAAGGCGAAGGAGCGAGTGACTGAGCGACGCGAGCCAGCAAGCCG
2761 tgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcagggg 2820
ACGCCGCTCGCCATAGTCGAGTGAGTTTCCGCCATTATGCCAATAGGTGTCTTAGTCCCC
2821 ataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaagg 2880
TATTGCGTCCTTTCTTGTACACTCGTTTTCCGGTCGTTTTCCGGTCCTTGGCATTTTTCC
pBR322 origin(2882,3501)<<<
|
2881 ccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgac 2940
GGCGCAACGACCGCAAAAAGGTATCCGAGGCGGGGGGACTGCTCGTAGTGTTTTTAGCTG
2941 gctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctg 3000
CGAGTTCAGTCTCCACCGCTTTGGGCTGTCCTGATATTTCTATGGTCCGCAAAGGGGGAC
3001 gaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcct 3060
CTTCGAGGGAGCACGCGAGAGGACAAGGCTGGGACGGCGAATGGCCTATGGACAGGCGGA
3061 ttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcgg 3120
AAGAGGGAAGCCCTTCGCACCGCGAAAGAGTATCGAGTGCGACATCCATAGAGTCAAGCC
3121 tgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgct 3180
ACATCCAGCAAGCGAGGTTCGACCCGACACACGTGCTTGGGGGGCAAGTCGGGCTGGCGA
3181 gcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccac 3240
CGCGGAATAGGCCATTGATAGCAGAACTCAGGTTGGGCCATTCTGTGCTGAATAGCGGTG
3241 tggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagt 3300
ACCGTCGTCGGTGACCATTGTCCTAATCGTCTCGCTCCATACATCCGCCACGATGTCTCA
3301 tcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctc 3360
AGAACTTCACCACCGGATTGATGCCGATGTGATCTTCCTGTCATAAACCATAGACGCGAG
3361 tgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaacca 3420
ACGACTTCGGTCAATGGAAGCCTTTTTCTCAACCATCGAGAACTAGGCCGTTTGTTTGGT
3421 ccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggat 3480
GGCGACCATCGCCACCAAAAAAACAAACGTTCGTCGTCTAATGCGCGTCTTTTTTTCCTA
3481 ctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcac 3540
GAGTTCTTCTAGGAAACTAGAAAAGATGCCCCAGACTGCGAGTCACCTTGCTTTTGAGTG
3541 gttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaatt 3600
CAATTCCCTAAAACCAGTACTCTAATAGTTTTTCCTAGAAGTGGATCTAGGAAAATTTAA
3601 aaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttacc 3660
TTTTTACTTCAAAATTTAGTTAGATTTCATATATACTCATTTGAACCAGACTGTCAATGG
3661 aatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttg 3720
TTACGAATTAGTCACTCCGTGGATAGAGTCGCTAGACAGATAAAGCAAGTAGGTATCAAC
3721 cctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtg 3780
GGACTGAGGGGCAGCACATCTATTGATGCTATGCCCTCCCGAATGGTAGACCGGGGTCAC
3781 ctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagc 3840
GACGTTACTATGGCGCTCTGGGTGCGAGTGGCCGAGGTCTAAATAGTCGTTATTTGGTCG
3841 cagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtcta 3900
GTCGGCCTTCCCGGCTCGCGTCTTCACCAGGACGTTGAAATAGGCGGAGGTAGGTCAGAT
3901 ttaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttg 3960
AATTAACAACGGCCCTTCGATCTCATTCATCAAGCGGTCAATTATCAAACGCGTTGCAAC
3961 ttgaaaaaggatcttcacctagatccttttcacgtagaaagccagtccgcagaaacggtg 4020
AACTTTTTCCTAGAAGTGGATCTAGGAAAAGTGCATCTTTCGGTCAGGCGTCTTTGCCAC
4021 ctgaccccggatgaatgtcagctactgggctatctggacaagggaaaacgcaagcgcaaa 4080
GACTGGGGCCTACTTACAGTCGATGACCCGATAGACCTGTTCCCTTTTGCGTTCGCGTTT
4081 gagaaagcaggtagcttgcagtgggcttacatggcgatagctagactgggcggttttatg 4140
CTCTTTCGTCCATCGAACGTCACCCGAATGTACCGCTATCGATCTGACCCGCCAAAATAC
NEOKAN prom(4154,4203)>>>
|
4141 gacagcaagcgaaccggaattgccagctggggcgccctctggtaaggttgggaagccctg 4200
CTGTCGTTCGCTTGGCCTTAACGGTCGACCCCGCGGGAGACCATTCCAACCCTTCGGGAC
4201 caaagtaaactggatggctttctcgccgccaaggatctgatggcgcaggggatcaagctc 4260
GTTTCATTTGACCTACCGAAAGAGCGGCGGTTCCTAGACTACCGCGTCCCCTAGTTCGAG
NTP_II marker(4295,5083)>>>
|
ORF_1 rf(2)(4292,5086)>>>
| |
4261 tgatcaagagacaggatgaggatcgtttcgcatgattgaacaagatggattgcacgcagg 4320
ACTAGTTCTCTGTCCTACTCCTAGCAAAGCGTACTAACTTGTTCTACCTAACGTGCGTCC
4321 ttctccggccgcttgggtggagaggctattcggctatgactgggcacaacagacaatcgg 4380
AAGAGGCCGGCGAACCCACCTCTCCGATAAGCCGATACTGACCCGTGTTGTCTGTTAGCC
4381 ctgctctgatgccgccgtgttccggctgtcagcgcaggggcgcccggttctttttgtcaa 4440
GACGAGACTACGGCGGCACAAGGCCGACAGTCGCGTCCCCGCGGGCCAAGAAAAACAGTT
4441 gaccgacctgtccggtgccctgaatgaactgcaagacgaggcagcgcggctatcgtggct 4500
CTGGCTGGACAGGCCACGGGACTTACTTGACGTTCTGCTCCGTCGCGCCGATAGCACCGA
4501 ggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactgaagcgggaaggga 4560
CCGGTGCTGCCCGCAAGGAACGCGTCGACACGAGCTGCAACAGTGACTTCGCCCTTCCCT
4561 ctggctgctattgggcgaagtgccggggcaggatctcctgtcatctcaccttgctcctgc 4620
GACCGACGATAACCCGCTTCACGGCCCCGTCCTAGAGGACAGTAGAGTGGAACGAGGACG
4621 cgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttgatccggctac 4680
GCTCTTTCATAGGTAGTACCGACTACGTTACGCCGCCGACGTATGCGAACTAGGCCGATG
4681 ctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaagc 4740
GACGGGTAAGCTGGTGGTTCGCTTTGTAGCGTAGCTCGCTCGTGCATGAGCCTACCTTCG
4741 cggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgccagccgaact 4800
GCCAGAACAGCTAGTCCTACTAGACCTGCTTCTCGTAGTCCCCGAGCGCGGTCGGCTTGA
4801 gttcgccaggctcaaggcgagcatgcccgacggcgaggatctcgtcgtgacccatggcga 4860
CAAGCGGTCCGAGTTCCGCTCGTACGGGCTGCCGCTCCTAGAGCAGCACTGGGTACCGCT
4861 tgcctgcttgccgaatatcatggtggaaaatggccgcttttctggattcatcgactgtgg 4920
ACGGACGAACGGCTTATAGTACCACCTTTTACCGGCGAAAAGACCTAAGTAGCTGACACC
4921 ccggctgggtgtggcggaccgctatcaggacatagcgttggctacccgtgatattgctga 4980
GGCCGACCCACACCGCCTGGCGATAGTCCTGTATCGCAACCGATGGGCACTATAACGACT
4981 agagcttggcggcgaatgggctgaccgcttcctcgtgctttacggtatcgccgctcccga 5040
TCTCGAACCGCCGCTTACCCGACTGGCGAAGGAGCACGAAATGCCATAGCGGCGAGGGCT
5041 ttcgcagcgcatcgccttctatcgccttcttgacgagttcttctgaattttgttaaaatt 5100
AAGCGTCGCGTAGCGGAAGATAGCGGAAGAACTGCTCAAGAAGACTTAAAACAATTTTAA
5101 tttgttaaatcagctcattttttaaccaataggccgaaatcggcaacatcccttataaat 5160
AAACAATTTAGTCGAGTAAAAAATTGGTTATCCGGCTTTAGCCGTTGTAGGGAATATTTA
f1 origin(5191,5496)<<<
|
5161 caaaagaatagaccgcgatagggttgagtgttgttccagtttggaacaagagtccactat 5220
GTTTTCTTATCTGGCGCTATCCCAACTCACAACAAGGTCAAACCTTGTTCTCAGGTGATA
5221 taaagaacgtggactccaacgtcaaagggcgaaaaaccgtctatcagggcgatggcccac 5280
ATTTCTTGCACCTGAGGTTGCAGTTTCCCGCTTTTTGGCAGATAGTCCCGCTACCGGGTG
5281 tacgtgaaccatcacccaaatcaagttttttgcggtcgaggtgccgtaaagctctaaatc 5340
ATGCACTTGGTAGTGGGTTTAGTTCAAAAAACGCCAGCTCCACGGCATTTCGAGATTTAG
5341 ggaaccctaaagggagcccccgatttagagcttgacggggaaagccggcgaacgtggcga 5400
CCTTGGGATTTCCCTCGGGGGCTAAATCTCGAACTGCCCCTTTCGGCCGCTTGCACCGCT
5401 gaaaggaagggaagaaagcgaaaggagcgggcgctagggcgctggcaagtgtagcggtca 5460
CTTTCCTTCCCTTCTTTCGCTTTCCTCGCCCGCGATCCCGCGACCGTTCACATCGCCAGT
5461 cgctgcgcgtaaccaccacacccgcgcgcttaatgcgccgctacagggcgcgtccattcg 5520
GCGACGCGCATTGGTGGTGTGGGCGCGCGAATTACGCGGCGATGTCCCGCGCAGGTAAGC
5521 ccattcaggatcgaattaattcttaattaacatcatcaataatatacctt 5570
GGTAAGTCCTAGCTTAATTAAGAATTAATTGTAGTAGTTATTATATGGAA