pShuttle(+)

Vector Name pShuttle(+)
VectorType Plasmid
Antibiotic Information Kanamycin
Sequencing Primers CMV sequencing primer
5`---CGC AAA TGG GCG GTA GGC GTG---3`
Additional Information No Additional Information
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector only and does not include the gene of interest. The sequence of the gene can be found through the accession number link on the product page. The restriction sites within the cloning region may differ from the provided sequence.
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt 61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa 121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg 181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt 241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg 301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag 361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta 481 gcgaaagctc agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc 541 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc 601 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct 661 tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg 721 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat 781 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc 841 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca 901 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta 961 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta 1021 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat 1081 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga 1141 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca 1201 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg 1261 taggcgtgta cggtgggagg tctatataag cagagctctc cctatcagtg atagagatct 1321 ccctatcagt gatagagatc gtcgacgagc tcgtttagtg aaccgtcaga tcgcctggag 1381 acgccatcca cgctgttttg acctccatag agctagcgtt taaacttaag cttggtaccg 1441 agctcggatc cactagtcca gtgtggtgga attctgcaga tatccagcac agtggcggcc 1501 gctcgagtct agagggcccg tttaaaccgc tgatcagcct cgactgtgcc ttctagttgc 1561 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc 1621 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct 1681 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg 1741 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag cagatcgatc 1801 tctatgtcgg gtgcggagaa agaggtaatg aaatggcatt atgggtatta tgggtctgca 1861 ttaatgaatc ggccaaggat ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga 1921 aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt 1981 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca 2041 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa 2101 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat 2161 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc 2221 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc 2281 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt 2341 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac 2401 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg 2461 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca 2521 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc 2581 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa 2641 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa 2701 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac 2761 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta 2821 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt 2881 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata 2941 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc 3001 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac 3061 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag 3121 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac 3181 gttgttgaaa aaggatcttc acctagatcc ttttcacgta gaaagccagt ccgcagaaac 3241 ggtgctgacc ccggatgaat gtcagctact gggctatctg gacaagggaa aacgcaagcg 3301 caaagagaaa gcaggtagct tgcagtgggc ttacatggcg atagctagac tgggcggttt 3361 tatggacagc aagcgaaccg gaattgccag ctggggcgcc ctctggtaag gttgggaagc 3421 cctgcaaagt aaactggatg gctttctcgc cgccaaggat ctgatggcgc aggggatcaa 3481 gctctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg 3541 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa 3601 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg 3661 tcaagaccga cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt 3721 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa 3781 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc 3841 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg 3901 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg 3961 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg 4021 aactgttcgc caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg 4081 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact 4141 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg 4201 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc 4261 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga attttgttaa 4321 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa catcccttat 4381 aaatcaaaag aatagaccgc gatagggttg agtgttgttc cagtttggaa caagagtcca 4441 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc 4501 ccactacgtg aaccatcacc caaatcaagt tttttgcggt cgaggtgccg taaagctcta 4561 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg 4621 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg 4681 gtcacgctgc gcgtaaccac cacacccgcg cgcttaatgc gccgctacag ggcgcgtcca 4741 ttcgccattc aggatcgaat taattcttaa ttaacatcat caataatata cctt
                            LOCUS dna 4794 bp FEATURES Location/Qualifiers Other Gene 162..310 /gene="Encap other" Promoter 365..393 /gene="amp prom" misc_binding 457..462 /dbxref="REBASE:HpaI" misc_binding 685..690 /dbxref="REBASE:NruI" Regulatory_Seq 1299..1338 /gene="tetO reg" misc_binding 1341..1346 /dbxref="REBASE:AccI" misc_binding 1428..1433 /dbxref="REBASE:HindIII" misc_binding 1434..1439 /dbxref="REBASE:KpnI" misc_binding 1469..1474 /dbxref="REBASE:EcoRI" misc_binding 1474..1479 /dbxref="REBASE:PstI" misc_binding 1479..1484 /dbxref="REBASE:EcoRV" misc_binding 1495..1502 /dbxref="REBASE:NotI" misc_binding 1502..1507 /dbxref="REBASE:AvrI" misc_binding 1502..1507 /dbxref="REBASE:AvaI" misc_binding 1502..1507 /dbxref="REBASE:XhoI" misc_binding 1508..1513 /dbxref="REBASE:XbaI" misc_binding 1514..1519 /dbxref="REBASE:ApaI" misc_binding 1794..1799 /dbxref="REBASE:ClaI" Rep_Origin 2106..2725 /gene="pBR322 origin" Promoter 3378..3427 /gene="NEOKAN prom" ORF 3516..4310 /sequence="ORF_1 rf(3)" Marker 3519..4307 /gene="NTP_II marker" Rep_Origin 4415..4720 /gene="f1 origin" BASE COUNT 1169 a 1161 c 1312 g 1152 t 0 others
                        
***Note: The text map shown below is representative of the parental vector only and does not include the gene of interest. The sequence of the gene can be found through the accession number link on the product page.
1 ttttggattgaagccaatatgataatgagggggtggagtttgtgacgtggcgcggggcgt 60 AAAACCTAACTTCGGTTATACTATTACTCCCCCACCTCAAACACTGCACCGCGCCCCGCA 61 gggaacggggcgggtgacgtagtagtgtggcggaagtgtgatgttgcaagtgtggcggaa 120 CCCTTGCCCCGCCCACTGCATCATCACACCGCCTTCACACTACAACGTTCACACCGCCTT Encap other(162,310)>>> | 121 cacatgtaagcgacggatgtggcaaaagtgacgtttttggtgtgcgccggtgtacacagg 180 GTGTACATTCGCTGCCTACACCGTTTTCACTGCAAAAACCACACGCGGCCACATGTGTCC 181 aagtgacaattttcgcgcggttttaggcggatgttgtagtaaatttgggcgtaaccgagt 240 TTCACTGTTAAAAGCGCGCCAAAATCCGCCTACAACATCATTTAAACCCGCATTGGCTCA 241 aagatttggccattttcgcgggaaaactgaataagaggaagtgaaatctgaataattttg 300 TTCTAAACCGGTAAAAGCGCCCTTTTGACTTATTCTCCTTCACTTTAGACTTATTAAAAC 301 tgttactcatagcgcgtaatacggcagacctcagcgctagattattgaagcatttatcag 360 ACAATGAGTATCGCGCATTATGCCGTCTGGAGTCGCGATCTAATAACTTCGTAAATAGTC amp prom(365,393)<<< | 361 ggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggg 420 CCAATAACAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTATCCC HpaI | 421 gttccgcgcacatttccccgaaaagtgccacctgacgttaactataacggtcctaaggta 480 CAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTGCAATTGATATTGCCAGGATTCCAT 481 gcgaaagctcagatctcccgatcccctatggtgcactctcagtacaatctgctctgatgc 540 CGCTTTCGAGTCTAGAGGGCTAGGGGATACCACGTGAGAGTCATGTTAGACGAGACTACG 541 cgcatagttaagccagtatctgctccctgcttgtgtgttggaggtcgctgagtagtgcgc 600 GCGTATCAATTCGGTCATAGACGAGGGACGAACACACAACCTCCAGCGACTCATCACGCG 601 gagcaaaatttaagctacaacaaggcaaggcttgaccgacaattgcatgaagaatctgct 660 CTCGTTTTAAATTCGATGTTGTTCCGTTCCGAACTGGCTGTTAACGTACTTCTTAGACGA NruI | 661 tagggttaggcgttttgcgctgcttcgcgatgtacgggccagatatacgcgttgacattg 720 ATCCCAATCCGCAAAACGCGACGAAGCGCTACATGCCCGGTCTATATGCGCAACTGTAAC 721 attattgactagttattaatagtaatcaattacggggtcattagttcatagcccatatat 780 TAATAACTGATCAATAATTATCATTAGTTAATGCCCCAGTAATCAAGTATCGGGTATATA 781 ggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgaccc 840 CCTCAAGGCGCAATGTATTGAATGCCATTTACCGGGCGGACCGACTGGCGGGTTGCTGGG 841 ccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttcca 900 GGCGGGTAACTGCAGTTATTACTGCATACAAGGGTATCATTGCGGTTATCCCTGAAAGGT 901 ttgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgta 960 AACTGCAGTTACCCACCTCATAAATGCCATTTGACGGGTGAACCGTCATGTAGTTCACAT 961 tcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcatta 1020 AGTATACGGTTCATGCGGGGGATAACTGCAGTTACTGCCATTTACCGGGCGGACCGTAAT 1021 tgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcat 1080 ACGGGTCATGTACTGGAATACCCTGAAAGGATGAACCGTCATGTAGATGCATAATCAGTA 1081 cgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttga 1140 GCGATAATGGTACCACTACGCCAAAACCGTCATGTAGTTACCCGCACCTATCGCCAAACT 1141 ctcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcacca 1200 GAGTGCCCCTAAAGGTTCAGAGGTGGGGTAACTGCAGTTACCCTCAAACAAAACCGTGGT 1201 aaatcaacgggactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcgg 1260 TTTAGTTGCCCTGAAAGGTTTTACAGCATTGTTGAGGCGGGGTAACTGCGTTTACCCGCC tetO reg(1299,1338)>>> | 1261 taggcgtgtacggtgggaggtctatataagcagagctctccctatcagtgatagagatct 1320 ATCCGCACATGCCACCCTCCAGATATATTCGTCTCGAGAGGGATAGTCACTATCTCTAGA AccI | 1321 ccctatcagtgatagagatcgtcgacgagctcgtttagtgaaccgtcagatcgcctggag 1380 GGGATAGTCACTATCTCTAGCAGCTGCTCGAGCAAATCACTTGGCAGTCTAGCGGACCTC HindIII KpnI | | 1381 acgccatccacgctgttttgacctccatagagctagcgtttaaacttaagcttggtaccg 1440 TGCGGTAGGTGCGACAAAACTGGAGGTATCTCGATCGCAAATTTGAATTCGAACCATGGC EcoRV | EcoRI PstI NotI | | | | 1441 agctcggatccactagtccagtgtggtggaattctgcagatatccagcacagtggcggcc 1500 TCGAGCCTAGGTGATCAGGTCACACCACCTTAAGACGTCTATAGGTCGTGTCACCGCCGG XhoI | AvaI | AvrI XbaI ApaI | | | 1501 gctcgagtctagagggcccgtttaaaccgctgatcagcctcgactgtgccttctagttgc 1560 CGAGCTCAGATCTCCCGGGCAAATTTGGCGACTAGTCGGAGCTGACACGGAAGATCAACG 1561 cagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactccc 1620 GTCGGTAGACAACAAACGGGGAGGGGGCACGGAAGGAACTGGGACCTTCCACGGTGAGGG 1621 actgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattct 1680 TGACAGGAAAGGATTATTTTACTCCTTTAACGTAGCGTAACAGACTCATCCACAGTAAGA 1681 attctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcagg 1740 TAAGACCCCCCACCCCACCCCGTCCTGTCGTTCCCCCTCCTAACCCTTCTGTTATCGTCC ClaI | 1741 catgctggggatgcggtgggctctatggcttctgaggcggaaagaaccagcagatcgatc 1800 GTACGACCCCTACGCCACCCGAGATACCGAAGACTCCGCCTTTCTTGGTCGTCTAGCTAG 1801 tctatgtcgggtgcggagaaagaggtaatgaaatggcattatgggtattatgggtctgca 1860 AGATACAGCCCACGCCTCTTTCTCCATTACTTTACCGTAATACCCATAATACCCAGACGT 1861 ttaatgaatcggccaaggatccatatgcggtgtgaaataccgcacagatgcgtaaggaga 1920 AATTACTTAGCCGGTTCCTAGGTATACGCCACACTTTATGGCGTGTCTACGCATTCCTCT 1921 aaataccgcatcaggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgtt 1980 TTTATGGCGTAGTCCGCGAGAAGGCGAAGGAGCGAGTGACTGAGCGACGCGAGCCAGCAA 1981 cggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatca 2040 GCCGACGCCGCTCGCCATAGTCGAGTGAGTTTCCGCCATTATGCCAATAGGTGTCTTAGT 2041 ggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaa 2100 CCCCTATTGCGTCCTTTCTTGTACACTCGTTTTCCGGTCGTTTTCCGGTCCTTGGCATTT pBR322 origin(2106,2725)<<< | 2101 aaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaat 2160 TTCCGGCGCAACGACCGCAAAAAGGTATCCGAGGCGGGGGGACTGCTCGTAGTGTTTTTA 2161 cgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccc 2220 GCTGCGAGTTCAGTCTCCACCGCTTTGGGCTGTCCTGATATTTCTATGGTCCGCAAAGGG 2221 cctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtcc 2280 GGACCTTCGAGGGAGCACGCGAGAGGACAAGGCTGGGACGGCGAATGGCCTATGGACAGG 2281 gcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagt 2340 CGGAAAGAGGGAAGCCCTTCGCACCGCGAAAGAGTATCGAGTGCGACATCCATAGAGTCA 2341 tcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgac 2400 AGCCACATCCAGCAAGCGAGGTTCGACCCGACACACGTGCTTGGGGGGCAAGTCGGGCTG 2401 cgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcg 2460 GCGACGCGGAATAGGCCATTGATAGCAGAACTCAGGTTGGGCCATTCTGTGCTGAATAGC 2461 ccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctaca 2520 GGTGACCGTCGTCGGTGACCATTGTCCTAATCGTCTCGCTCCATACATCCGCCACGATGT 2521 gagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgc 2580 CTCAAGAACTTCACCACCGGATTGATGCCGATGTGATCTTCCTGTCATAAACCATAGACG 2581 gctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaa 2640 CGAGACGACTTCGGTCAATGGAAGCCTTTTTCTCAACCATCGAGAACTAGGCCGTTTGTT 2641 accaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaa 2700 TGGTGGCGACCATCGCCACCAAAAAAACAAACGTTCGTCGTCTAATGCGCGTCTTTTTTT 2701 ggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaac 2760 CCTAGAGTTCTTCTAGGAAACTAGAAAAGATGCCCCAGACTGCGAGTCACCTTGCTTTTG 2761 tcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatcctttta 2820 AGTGCAATTCCCTAAAACCAGTACTCTAATAGTTTTTCCTAGAAGTGGATCTAGGAAAAT 2821 aattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagt 2880 TTAATTTTTACTTCAAAATTTAGTTAGATTTCATATATACTCATTTGAACCAGACTGTCA 2881 taccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccata 2940 ATGGTTACGAATTAGTCACTCCGTGGATAGAGTCGCTAGACAGATAAAGCAAGTAGGTAT 2941 gttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggcccc 3000 CAACGGACTGAGGGGCAGCACATCTATTGATGCTATGCCCTCCCGAATGGTAGACCGGGG 3001 agtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaac 3060 TCACGACGTTACTATGGCGCTCTGGGTGCGAGTGGCCGAGGTCTAAATAGTCGTTATTTG 3061 cagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccag 3120 GTCGGTCGGCCTTCCCGGCTCGCGTCTTCACCAGGACGTTGAAATAGGCGGAGGTAGGTC 3121 tctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaac 3180 AGATAATTAACAACGGCCCTTCGATCTCATTCATCAAGCGGTCAATTATCAAACGCGTTG 3181 gttgttgaaaaaggatcttcacctagatccttttcacgtagaaagccagtccgcagaaac 3240 CAACAACTTTTTCCTAGAAGTGGATCTAGGAAAAGTGCATCTTTCGGTCAGGCGTCTTTG 3241 ggtgctgaccccggatgaatgtcagctactgggctatctggacaagggaaaacgcaagcg 3300 CCACGACTGGGGCCTACTTACAGTCGATGACCCGATAGACCTGTTCCCTTTTGCGTTCGC 3301 caaagagaaagcaggtagcttgcagtgggcttacatggcgatagctagactgggcggttt 3360 GTTTCTCTTTCGTCCATCGAACGTCACCCGAATGTACCGCTATCGATCTGACCCGCCAAA NEOKAN prom(3378,3427)>>> | 3361 tatggacagcaagcgaaccggaattgccagctggggcgccctctggtaaggttgggaagc 3420 ATACCTGTCGTTCGCTTGGCCTTAACGGTCGACCCCGCGGGAGACCATTCCAACCCTTCG 3421 cctgcaaagtaaactggatggctttctcgccgccaaggatctgatggcgcaggggatcaa 3480 GGACGTTTCATTTGACCTACCGAAAGAGCGGCGGTTCCTAGACTACCGCGTCCCCTAGTT NTP_II marker(3519,4307)>>> | ORF_1 rf(3)(3516,4310)>>> | | 3481 gctctgatcaagagacaggatgaggatcgtttcgcatgattgaacaagatggattgcacg 3540 CGAGACTAGTTCTCTGTCCTACTCCTAGCAAAGCGTACTAACTTGTTCTACCTAACGTGC 3541 caggttctccggccgcttgggtggagaggctattcggctatgactgggcacaacagacaa 3600 GTCCAAGAGGCCGGCGAACCCACCTCTCCGATAAGCCGATACTGACCCGTGTTGTCTGTT 3601 tcggctgctctgatgccgccgtgttccggctgtcagcgcaggggcgcccggttctttttg 3660 AGCCGACGAGACTACGGCGGCACAAGGCCGACAGTCGCGTCCCCGCGGGCCAAGAAAAAC 3661 tcaagaccgacctgtccggtgccctgaatgaactgcaagacgaggcagcgcggctatcgt 3720 AGTTCTGGCTGGACAGGCCACGGGACTTACTTGACGTTCTGCTCCGTCGCGCCGATAGCA 3721 ggctggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactgaagcgggaa 3780 CCGACCGGTGCTGCCCGCAAGGAACGCGTCGACACGAGCTGCAACAGTGACTTCGCCCTT 3781 gggactggctgctattgggcgaagtgccggggcaggatctcctgtcatctcaccttgctc 3840 CCCTGACCGACGATAACCCGCTTCACGGCCCCGTCCTAGAGGACAGTAGAGTGGAACGAG 3841 ctgccgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttgatccgg 3900 GACGGCTCTTTCATAGGTAGTACCGACTACGTTACGCCGCCGACGTATGCGAACTAGGCC 3901 ctacctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatgg 3960 GATGGACGGGTAAGCTGGTGGTTCGCTTTGTAGCGTAGCTCGCTCGTGCATGAGCCTACC 3961 aagccggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgccagccg 4020 TTCGGCCAGAACAGCTAGTCCTACTAGACCTGCTTCTCGTAGTCCCCGAGCGCGGTCGGC 4021 aactgttcgccaggctcaaggcgagcatgcccgacggcgaggatctcgtcgtgacccatg 4080 TTGACAAGCGGTCCGAGTTCCGCTCGTACGGGCTGCCGCTCCTAGAGCAGCACTGGGTAC 4081 gcgatgcctgcttgccgaatatcatggtggaaaatggccgcttttctggattcatcgact 4140 CGCTACGGACGAACGGCTTATAGTACCACCTTTTACCGGCGAAAAGACCTAAGTAGCTGA 4141 gtggccggctgggtgtggcggaccgctatcaggacatagcgttggctacccgtgatattg 4200 CACCGGCCGACCCACACCGCCTGGCGATAGTCCTGTATCGCAACCGATGGGCACTATAAC 4201 ctgaagagcttggcggcgaatgggctgaccgcttcctcgtgctttacggtatcgccgctc 4260 GACTTCTCGAACCGCCGCTTACCCGACTGGCGAAGGAGCACGAAATGCCATAGCGGCGAG 4261 ccgattcgcagcgcatcgccttctatcgccttcttgacgagttcttctgaattttgttaa 4320 GGCTAAGCGTCGCGTAGCGGAAGATAGCGGAAGAACTGCTCAAGAAGACTTAAAACAATT 4321 aatttttgttaaatcagctcattttttaaccaataggccgaaatcggcaacatcccttat 4380 TTAAAAACAATTTAGTCGAGTAAAAAATTGGTTATCCGGCTTTAGCCGTTGTAGGGAATA f1 origin(4415,4720)<<< | 4381 aaatcaaaagaatagaccgcgatagggttgagtgttgttccagtttggaacaagagtcca 4440 TTTAGTTTTCTTATCTGGCGCTATCCCAACTCACAACAAGGTCAAACCTTGTTCTCAGGT 4441 ctattaaagaacgtggactccaacgtcaaagggcgaaaaaccgtctatcagggcgatggc 4500 GATAATTTCTTGCACCTGAGGTTGCAGTTTCCCGCTTTTTGGCAGATAGTCCCGCTACCG 4501 ccactacgtgaaccatcacccaaatcaagttttttgcggtcgaggtgccgtaaagctcta 4560 GGTGATGCACTTGGTAGTGGGTTTAGTTCAAAAAACGCCAGCTCCACGGCATTTCGAGAT 4561 aatcggaaccctaaagggagcccccgatttagagcttgacggggaaagccggcgaacgtg 4620 TTAGCCTTGGGATTTCCCTCGGGGGCTAAATCTCGAACTGCCCCTTTCGGCCGCTTGCAC 4621 gcgagaaaggaagggaagaaagcgaaaggagcgggcgctagggcgctggcaagtgtagcg 4680 CGCTCTTTCCTTCCCTTCTTTCGCTTTCCTCGCCCGCGATCCCGCGACCGTTCACATCGC 4681 gtcacgctgcgcgtaaccaccacacccgcgcgcttaatgcgccgctacagggcgcgtcca 4740 CAGTGCGACGCGCATTGGTGGTGTGGGCGCGCGAATTACGCGGCGATGTCCCGCGCAGGT 4741 ttcgccattcaggatcgaattaattcttaattaacatcatcaataatatacctt 4794 AAGCGGTAAGTCCTAGCTTAATTAAGAATTAATTGTAGTAGTTATTATATGGAA