pRetro-Easy2

Vector Name	pRetro-Easy2
VectorType	Retroviral Vector
Antibiotic Information	Ampicillin
Sequencing Primers	CMV sequencing primer 5`---CGC AAA TGG GCG GTA GGC GTG---3`
Additional Information	No Additional Information

***Note: Features may not be to scale, please refer to sequence or text map for detailed information.

***Note: The vector sequence shown below is representative of the parental vector only and does not include the gene of interest. The sequence of the gene can be found through the accession number link on the product page. The restriction sites within the cloning region may differ from the provided sequence.

1 tttgaaagac cccacccgta ggtggcaagc tagcttaagt aacgccactt tgcaaggcat 
61 ggaaaaatac ataactgaga atagaaaagt tcagatcaag gtcaggaaca aagaaacagc 
121 tgaataccaa acaggatatc tgtggtaagc ggttcctgcc ccggctcagg gccaagaaca 
181 gatgagacag ctgagtgatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg 
241 ctcggggcca agaacagatg gtccccagat gcggtccagc cctcagcagt ttctagtgaa 
301 tcatcagatg tttccagggt gccccaagga cctgaaaatg accctgtacc ttatttgaac 
361 taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc cgctctccga gctcaataaa 
421 agagcccaca acccctcact cggcgcgcca gtcttccgat agactgcgtc gcccgggtac 
481 ccgtattccc aataaagcct cttgctgttt gcatccgaat cgtggtctcg ctgttccttg 
541 ggagggtctc ctctgagtga ttgactaccc acgacggggg tctttcattt gggggctcgt 
601 ccgggatttg gagacccctg cccagggacc accgacccac caccgggagg taagctggcc 
661 agcaacttat ctgtgtctgt ccgattgtct agtgtctatg tttgatgtta tgcgcctgcg 
721 tctgtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa ctgacgagtt 
781 ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc gtttttgtgg 
841 cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg tggttctggt 
901 aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt cggtttggaa 
961 ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct 
1021 gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc ccttaagttt 
1081 gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa 
1141 gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg tcggatggcc 
1201 gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg tcttttcacc 
1261 tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag ccttggcttt 
1321 tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc ctcttcctcc 
1381 atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta 
1441 tccagccctc actccttctc taggcgccgg aattgatccc ccgggctgca ggtcggccgc 
1501 cacgaccggt gccgccacca tcccctgacc cacgcccctg acccctcaca aggagacgac 
1561 cttccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtcccccggg 
1621 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgacc 
1681 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc 
1741 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc 
1801 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga 
1861 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca 
1921 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc 
1981 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct 
2041 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca 
2101 ccgccgacgt cgagtgcccg aaggaccgcg cgacctggtg catgacccgc aagcccggtg 
2161 cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga ccccatggct 
2221 ccgaccgaag ccgacccggg cggccccgcc gaccccgcac ccgcccccga ggcccaccga 
2281 ctctagtaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc 
2341 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg 
2401 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa 
2461 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca 
2521 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac 
2581 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc 
2641 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga 
2701 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg 
2761 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta 
2821 cggtgggagg tctatataag cagagctggt ttagtgaacc gtcagatccg ctagcgctac 
2881 cggactcaga tctcgagggg cccggatccg ttaacgcatg catttaaata agcttcgaat 
2941 tcgttaggcc attaaggcct gtcgacaagc ggccgcctcg gccaaacatc gataaaataa 
3001 aagattttat ttagtctcca gaaaaagggg ggaatgaaag accccacctg taggtttggc 
3061 aagctagctt aagtaacgcc attttgcaag gcatggaaaa atacataact gagaatagag 
3121 aagttcagat caaggtcagg aacagatgga acagctgaat atgggccaaa caggatatct 
3181 gtggtaagca gttcctgccc cggctcaggg ccaagaacag atggaacagc tgaatatggg 
3241 ccaaacagga tatctgtggt aagcagttcc tgccccggct cagggccaag aacagatggt 
3301 ccccagatgc ggtccagccc tcagcagttt ctagagaacc atcagatgtt tccagggtgc 
3361 cccaaggacc tgaaatgacc ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc 
3421 ttctgttcgc gcgcttctgc tccccgagct caataaaaga gcccacaacc cctcactcgg 
3481 ggcgccagtc ctccgattga ctgagtcgcc cgggtacccg tgtatccaat aaaccctctt 
3541 gcagttgcat ccgacttgtg gtctcgctgt tccttgggag ggtctcctct gagtgattga 
3601 ctacccgtca gcgggggtct ttcatttggg ggctcgtccg ggatcgggag acccctgccc 
3661 agggaccacc gacccaccac cgggaggtaa gctggctgcc tcgcgcgttt cggtgatgac 
3721 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat 
3781 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca 
3841 gccatgaccc agtcacgtag cgatagcgga gtgtactgat aacttcgtat aatgtatgct 
3901 atacgaagtt attaggtctg aagaggagtt tacgtccagc caagctaggg ccgcgatccg 
3961 gaacccttaa tataacttcg tataatgtat gctatacgaa gttatcagta ctggcttaac 
4021 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac 
4081 agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg 
4141 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg 
4201 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag 
4261 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac 
4321 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga 
4381 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt 
4441 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc 
4501 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc 
4561 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta 
4621 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat 
4681 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca 
4741 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct 
4801 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt 
4861 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct 
4921 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc 
4981 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa 
5041 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta 
5101 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc 
5161 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat 
5221 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta 
5281 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt 
5341 aatagtttgc gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt 
5401 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg 
5461 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc 
5521 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc 
5581 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg 
5641 cggcgaccga gttgctcttg cccggcgtca acacgggata ataccgcgcc acatagcaga 
5701 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta 
5761 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct 
5821 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag 
5881 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga 
5941 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat 
6001 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc 
6061 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtcttcaa 
6121 gaattgctag caattgctag caattgctag caattcatac cagatcaccg aaaactgtcc 
6181 tccaaatgtg tccccctcac actcccaaat tcgcgggctt ctgcctctta gaccactcta 
6241 ccctattccc cacactcacc ggagccaaag ccgcggccct tccgtttctt tgct

                            LOCUS dna 6294 bp 
FEATURES Location/Qualifiers
Other Gene 659..1468
/gene="psi_plus_pack other"
ORF 1449..2216
/sequence="ORF_2 rf(5)"
Marker 1566..2165
/gene="puro marker"
Promoter 2787..2868
/gene="CMV prom"
misc_binding 2888..2893
/dbxref="REBASE:BglII"
misc_binding 2892..2897
/dbxref="REBASE:XhoI"
misc_binding 2898..2903
/dbxref="REBASE:ApaI"
misc_binding 2904..2909
/dbxref="REBASE:BamHI"
misc_binding 2910..2915
/dbxref="REBASE:HpaI"
misc_binding 2916..2921
/dbxref="REBASE:SphI"
misc_binding 2930..2935
/dbxref="REBASE:HindIII"
misc_binding 2937..2942
/dbxref="REBASE:EcoRI"
misc_binding 2969..2976
/dbxref="REBASE:NotI"
misc_binding 2988..2993
/dbxref="REBASE:ClaI"
Other Gene 3075..3626
/gene="5_LTR2 other"
misc_binding 3330..3335
/dbxref="REBASE:XbaI"
Other Gene 3879..3912
/gene="loxP other"
Other Gene 3972..4005
/gene="loxP other"
Rep_Origin 4281..4900
/gene="pBR322 origin"
Marker 5055..5915
/gene="amp marker"
ORF 5055..5915
/sequence="ORF_1 rf(5)"
Promoter 5957..5985
/gene="amp prom"
BASE COUNT 1448 a 1812 c 1607 g 1427 t 0 others
ORIGIN
1 tttgaaagac cccacccgta ggtggcaagc tagcttaagt aacgccactt tgcaaggcat 
61 ggaaaaatac ataactgaga atagaaaagt tcagatcaag gtcaggaaca aagaaacagc 
121 tgaataccaa acaggatatc tgtggtaagc ggttcctgcc ccggctcagg gccaagaaca 
181 gatgagacag ctgagtgatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg 
241 ctcggggcca agaacagatg gtccccagat gcggtccagc cctcagcagt ttctagtgaa 
301 tcatcagatg tttccagggt gccccaagga cctgaaaatg accctgtacc ttatttgaac 
361 taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc cgctctccga gctcaataaa 
421 agagcccaca acccctcact cggcgcgcca gtcttccgat agactgcgtc gcccgggtac 
481 ccgtattccc aataaagcct cttgctgttt gcatccgaat cgtggtctcg ctgttccttg 
541 ggagggtctc ctctgagtga ttgactaccc acgacggggg tctttcattt gggggctcgt 
601 ccgggatttg gagacccctg cccagggacc accgacccac caccgggagg taagctggcc 
661 agcaacttat ctgtgtctgt ccgattgtct agtgtctatg tttgatgtta tgcgcctgcg 
721 tctgtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa ctgacgagtt 
781 ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc gtttttgtgg 
841 cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg tggttctggt 
901 aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt cggtttggaa 
961 ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct 
1021 gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc ccttaagttt 
1081 gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa 
1141 gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg tcggatggcc 
1201 gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg tcttttcacc 
1261 tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag ccttggcttt 
1321 tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc ctcttcctcc 
1381 atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta 
1441 tccagccctc actccttctc taggcgccgg aattgatccc ccgggctgca ggtcggccgc 
1501 cacgaccggt gccgccacca tcccctgacc cacgcccctg acccctcaca aggagacgac 
1561 cttccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtcccccggg 
1621 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgacc 
1681 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc 
1741 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc 
1801 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga 
1861 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca 
1921 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc 
1981 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct 
2041 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca 
2101 ccgccgacgt cgagtgcccg aaggaccgcg cgacctggtg catgacccgc aagcccggtg 
2161 cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga ccccatggct 
2221 ccgaccgaag ccgacccggg cggccccgcc gaccccgcac ccgcccccga ggcccaccga 
2281 ctctagtaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc 
2341 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg 
2401 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa 
2461 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca 
2521 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac 
2581 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc 
2641 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga 
2701 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg 
2761 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta 
2821 cggtgggagg tctatataag cagagctggt ttagtgaacc gtcagatccg ctagcgctac 
2881 cggactcaga tctcgagggg cccggatccg ttaacgcatg catttaaata agcttcgaat 
2941 tcgttaggcc attaaggcct gtcgacaagc ggccgcctcg gccaaacatc gataaaataa 
3001 aagattttat ttagtctcca gaaaaagggg ggaatgaaag accccacctg taggtttggc 
3061 aagctagctt aagtaacgcc attttgcaag gcatggaaaa atacataact gagaatagag 
3121 aagttcagat caaggtcagg aacagatgga acagctgaat atgggccaaa caggatatct 
3181 gtggtaagca gttcctgccc cggctcaggg ccaagaacag atggaacagc tgaatatggg 
3241 ccaaacagga tatctgtggt aagcagttcc tgccccggct cagggccaag aacagatggt 
3301 ccccagatgc ggtccagccc tcagcagttt ctagagaacc atcagatgtt tccagggtgc 
3361 cccaaggacc tgaaatgacc ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc 
3421 ttctgttcgc gcgcttctgc tccccgagct caataaaaga gcccacaacc cctcactcgg 
3481 ggcgccagtc ctccgattga ctgagtcgcc cgggtacccg tgtatccaat aaaccctctt 
3541 gcagttgcat ccgacttgtg gtctcgctgt tccttgggag ggtctcctct gagtgattga 
3601 ctacccgtca gcgggggtct ttcatttggg ggctcgtccg ggatcgggag acccctgccc 
3661 agggaccacc gacccaccac cgggaggtaa gctggctgcc tcgcgcgttt cggtgatgac 
3721 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat 
3781 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca 
3841 gccatgaccc agtcacgtag cgatagcgga gtgtactgat aacttcgtat aatgtatgct 
3901 atacgaagtt attaggtctg aagaggagtt tacgtccagc caagctaggg ccgcgatccg 
3961 gaacccttaa tataacttcg tataatgtat gctatacgaa gttatcagta ctggcttaac 
4021 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac 
4081 agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg 
4141 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg 
4201 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag 
4261 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac 
4321 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga 
4381 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt 
4441 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc 
4501 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc 
4561 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta 
4621 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat 
4681 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca 
4741 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct 
4801 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt 
4861 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct 
4921 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc 
4981 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa 
5041 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta 
5101 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc 
5161 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat 
5221 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta 
5281 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt 
5341 aatagtttgc gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt 
5401 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg 
5461 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc 
5521 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc 
5581 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg 
5641 cggcgaccga gttgctcttg cccggcgtca acacgggata ataccgcgcc acatagcaga 
5701 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta 
5761 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct 
5821 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag 
5881 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga 
5941 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat 
6001 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc 
6061 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtcttcaa 
6121 gaattgctag caattgctag caattgctag caattcatac cagatcaccg aaaactgtcc 
6181 tccaaatgtg tccccctcac actcccaaat tcgcgggctt ctgcctctta gaccactcta 
6241 ccctattccc cacactcacc ggagccaaag ccgcggccct tccgtttctt tgct 
//

***Note: The text map shown below is representative of the parental vector only and does not include the gene of interest. The sequence of the gene can be found through the accession number link on the product page.

1 tttgaaagaccccacccgtaggtggcaagctagcttaagtaacgccactttgcaaggcat 60
AAACTTTCTGGGGTGGGCATCCACCGTTCGATCGAATTCATTGCGGTGAAACGTTCCGTA

61 ggaaaaatacataactgagaatagaaaagttcagatcaaggtcaggaacaaagaaacagc 120
CCTTTTTATGTATTGACTCTTATCTTTTCAAGTCTAGTTCCAGTCCTTGTTTCTTTGTCG

121 tgaataccaaacaggatatctgtggtaagcggttcctgccccggctcagggccaagaaca 180
ACTTATGGTTTGTCCTATAGACACCATTCGCCAAGGACGGGGCCGAGTCCCGGTTCTTGT

181 gatgagacagctgagtgatgggccaaacaggatatctgtggtaagcagttcctgccccgg 240
CTACTCTGTCGACTCACTACCCGGTTTGTCCTATAGACACCATTCGTCAAGGACGGGGCC

241 ctcggggccaagaacagatggtccccagatgcggtccagccctcagcagtttctagtgaa 300
GAGCCCCGGTTCTTGTCTACCAGGGGTCTACGCCAGGTCGGGAGTCGTCAAAGATCACTT

301 tcatcagatgtttccagggtgccccaaggacctgaaaatgaccctgtaccttatttgaac 360
AGTAGTCTACAAAGGTCCCACGGGGTTCCTGGACTTTTACTGGGACATGGAATAAACTTG

361 taaccaatcagttcgcttctcgcttctgttcgcgcgcttccgctctccgagctcaataaa 420
ATTGGTTAGTCAAGCGAAGAGCGAAGACAAGCGCGCGAAGGCGAGAGGCTCGAGTTATTT

421 agagcccacaacccctcactcggcgcgccagtcttccgatagactgcgtcgcccgggtac 480
TCTCGGGTGTTGGGGAGTGAGCCGCGCGGTCAGAAGGCTATCTGACGCAGCGGGCCCATG

481 ccgtattcccaataaagcctcttgctgtttgcatccgaatcgtggtctcgctgttccttg 540
GGCATAAGGGTTATTTCGGAGAACGACAAACGTAGGCTTAGCACCAGAGCGACAAGGAAC

541 ggagggtctcctctgagtgattgactacccacgacgggggtctttcatttgggggctcgt 600
CCTCCCAGAGGAGACTCACTAACTGATGGGTGCTGCCCCCAGAAAGTAAACCCCCGAGCA

psi_plus_pack other(659,1468)>>> 
|
601 ccgggatttggagacccctgcccagggaccaccgacccaccaccgggaggtaagctggcc 660
GGCCCTAAACCTCTGGGGACGGGTCCCTGGTGGCTGGGTGGTGGCCCTCCATTCGACCGG

661 agcaacttatctgtgtctgtccgattgtctagtgtctatgtttgatgttatgcgcctgcg 720
TCGTTGAATAGACACAGACAGGCTAACAGATCACAGATACAAACTACAATACGCGGACGC

721 tctgtactagttagctaactagctctgtatctggcggacccgtggtggaactgacgagtt 780
AGACATGATCAATCGATTGATCGAGACATAGACCGCCTGGGCACCACCTTGACTGCTCAA

781 ctgaacacccggccgcaaccctgggagacgtcccagggactttgggggccgtttttgtgg 840
GACTTGTGGGCCGGCGTTGGGACCCTCTGCAGGGTCCCTGAAACCCCCGGCAAAAACACC

841 cccgacctgaggaagggagtcgatgtggaatccgaccccgtcaggatatgtggttctggt 900
GGGCTGGACTCCTTCCCTCAGCTACACCTTAGGCTGGGGCAGTCCTATACACCAAGACCA

901 aggagacgagaacctaaaacagttcccgcctccgtctgaatttttgctttcggtttggaa 960
TCCTCTGCTCTTGGATTTTGTCAAGGGCGGAGGCAGACTTAAAAACGAAAGCCAAACCTT

961 ccgaagccgcgcgtcttgtctgctgcagcgctgcagcatcgttctgtgttgtctctgtct 1020
GGCTTCGGCGCGCAGAACAGACGACGTCGCGACGTCGTAGCAAGACACAACAGAGACAGA

1021 gactgtgtttctgtatttgtctgaaaattagggccagactgttaccactcccttaagttt 1080
CTGACACAAAGACATAAACAGACTTTTAATCCCGGTCTGACAATGGTGAGGGAATTCAAA

1081 gaccttaggtcactggaaagatgtcgagcggatcgctcacaaccagtcggtagatgtcaa 1140
CTGGAATCCAGTGACCTTTCTACAGCTCGCCTAGCGAGTGTTGGTCAGCCATCTACAGTT

1141 gaagagacgttgggttaccttctgctctgcagaatggccaacctttaacgtcggatggcc 1200
CTTCTCTGCAACCCAATGGAAGACGAGACGTCTTACCGGTTGGAAATTGCAGCCTACCGG

1201 gcgagacggcacctttaaccgagacctcatcacccaggttaagatcaaggtcttttcacc 1260
CGCTCTGCCGTGGAAATTGGCTCTGGAGTAGTGGGTCCAATTCTAGTTCCAGAAAAGTGG

1261 tggcccgcatggacacccagaccaggtcccctacatcgtgacctgggaagccttggcttt 1320
ACCGGGCGTACCTGTGGGTCTGGTCCAGGGGATGTAGCACTGGACCCTTCGGAACCGAAA

1321 tgacccccctccctgggtcaagccctttgtacaccctaagcctccgcctcctcttcctcc 1380
ACTGGGGGGAGGGACCCAGTTCGGGAAACATGTGGGATTCGGAGGCGGAGGAGAAGGAGG

1381 atccgccccgtctctcccccttgaacctcctcgttcgaccccgcctcgatcctcccttta 1440
TAGGCGGGGCAGAGAGGGGGAACTTGGAGGAGCAAGCTGGGGCGGAGCTAGGAGGGAAAT

ORF_2 rf(5)(1449,2216)<<< 
|
1441 tccagccctcactccttctctaggcgccggaattgatcccccgggctgcaggtcggccgc 1500
AGGTCGGGAGTGAGGAAGAGATCCGCGGCCTTAACTAGGGGGCCCGACGTCCAGCCGGCG

1501 cacgaccggtgccgccaccatcccctgacccacgcccctgacccctcacaaggagacgac 1560
GTGCTGGCCACGGCGGTGGTAGGGGACTGGGTGCGGGGACTGGGGAGTGTTCCTCTGCTG

puro marker(1566,2165)>>> 
|
1561 cttccatgaccgagtacaagcccacggtgcgcctcgccacccgcgacgacgtcccccggg 1620
GAAGGTACTGGCTCATGTTCGGGTGCCACGCGGAGCGGTGGGCGCTGCTGCAGGGGGCCC

1621 ccgtacgcaccctcgccgccgcgttcgccgactaccccgccacgcgccacaccgtcgacc 1680
GGCATGCGTGGGAGCGGCGGCGCAAGCGGCTGATGGGGCGGTGCGCGGTGTGGCAGCTGG

1681 cggaccgccacatcgagcgggtcaccgagctgcaagaactcttcctcacgcgcgtcgggc 1740
GCCTGGCGGTGTAGCTCGCCCAGTGGCTCGACGTTCTTGAGAAGGAGTGCGCGCAGCCCG

1741 tcgacatcggcaaggtgtgggtcgcggacgacggcgccgcggtggcggtctggaccacgc 1800
AGCTGTAGCCGTTCCACACCCAGCGCCTGCTGCCGCGGCGCCACCGCCAGACCTGGTGCG

1801 cggagagcgtcgaagcgggggcggtgttcgccgagatcggcccgcgcatggccgagttga 1860
GCCTCTCGCAGCTTCGCCCCCGCCACAAGCGGCTCTAGCCGGGCGCGTACCGGCTCAACT

1861 gcggttcccggctggccgcgcagcaacagatggaaggcctcctggcgccgcaccggccca 1920
CGCCAAGGGCCGACCGGCGCGTCGTTGTCTACCTTCCGGAGGACCGCGGCGTGGCCGGGT

1921 aggagcccgcgtggttcctggccaccgtcggcgtctcgcccgaccaccagggcaagggtc 1980
TCCTCGGGCGCACCAAGGACCGGTGGCAGCCGCAGAGCGGGCTGGTGGTCCCGTTCCCAG

1981 tgggcagcgccgtcgtgctccccggagtggaggcggccgagcgcgccggggtgcccgcct 2040
ACCCGTCGCGGCAGCACGAGGGGCCTCACCTCCGCCGGCTCGCGCGGCCCCACGGGCGGA

2041 tcctggagacctccgcgccccgcaacctccccttctacgagcggctcggcttcaccgtca 2100
AGGACCTCTGGAGGCGCGGGGCGTTGGAGGGGAAGATGCTCGCCGAGCCGAAGTGGCAGT

2101 ccgccgacgtcgagtgcccgaaggaccgcgcgacctggtgcatgacccgcaagcccggtg 2160
GGCGGCTGCAGCTCACGGGCTTCCTGGCGCGCTGGACCACGTACTGGGCGTTCGGGCCAC

2161 cctgacgcccgccccacgacccgcagcgcccgaccgaaaggagcgcacgaccccatggct 2220
GGACTGCGGGCGGGGTGCTGGGCGTCGCGGGCTGGCTTTCCTCGCGTGCTGGGGTACCGA

2221 ccgaccgaagccgacccgggcggccccgccgaccccgcacccgcccccgaggcccaccga 2280
GGCTGGCTTCGGCTGGGCCCGCCGGGGCGGCTGGGGCGTGGGCGGGGGCTCCGGGTGGCT

2281 ctctagtaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgc 2340
GAGATCATTATCATTAGTTAATGCCCCAGTAATCAAGTATCGGGTATATACCTCAAGGCG

2341 gttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattg 2400
CAATGTATTGAATGCCATTTACCGGGCGGACCGACTGGCGGGTTGCTGGGGGCGGGTAAC

2401 acgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaa 2460
TGCAGTTATTACTGCATACAAGGGTATCATTGCGGTTATCCCTGAAAGGTAACTGCAGTT

2461 tgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgcca 2520
ACCCACCTCATAAATGCCATTTGACGGGTGAACCGTCATGTAGTTCACATAGTATACGGT

2521 agtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtac 2580
TCATGCGGGGGATAACTGCAGTTACTGCCATTTACCGGGCGGACCGTAATACGGGTCATG

2581 atgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattacc 2640
TACTGGAATACCCTGAAAGGATGAACCGTCATGTAGATGCATAATCAGTAGCGATAATGG

2641 atggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacgggga 2700
TACCACTACGCCAAAACCGTCATGTAGTTACCCGCACCTATCGCCAAACTGAGTGCCCCT

2701 tttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgg 2760
AAAGGTTCAGAGGTGGGGTAACTGCAGTTACCCTCAAACAAAACCGTGGTTTTAGTTGCC

CMV prom(2787,2868)>>> 
|
2761 gactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgta 2820
CTGAAAGGTTTTACAGCATTGTTGAGGCGGGGTAACTGCGTTTACCCGCCATCCGCACAT

2821 cggtgggaggtctatataagcagagctggtttagtgaaccgtcagatccgctagcgctac 2880
GCCACCCTCCAGATATATTCGTCTCGACCAAATCACTTGGCAGTCTAGGCGATCGCGATG

XhoI BamHI EcoRI 
| | |
BglII ApaI HpaI SphI HindIII 
| | | | | | | |
2881 cggactcagatctcgaggggcccggatccgttaacgcatgcatttaaataagcttcgaat 2940
GCCTGAGTCTAGAGCTCCCCGGGCCTAGGCAATTGCGTACGTAAATTTATTCGAAGCTTA

NotI ClaI 
| |
2941 tcgttaggccattaaggcctgtcgacaagcggccgcctcggccaaacatcgataaaataa 3000
AGCAATCCGGTAATTCCGGACAGCTGTTCGCCGGCGGAGCCGGTTTGTAGCTATTTTATT

3001 aagattttatttagtctccagaaaaaggggggaatgaaagaccccacctgtaggtttggc 3060
TTCTAAAATAAATCAGAGGTCTTTTTCCCCCCTTACTTTCTGGGGTGGACATCCAAACCG

5_LTR2 other(3075,3626)>>> 
|
3061 aagctagcttaagtaacgccattttgcaaggcatggaaaaatacataactgagaatagag 3120
TTCGATCGAATTCATTGCGGTAAAACGTTCCGTACCTTTTTATGTATTGACTCTTATCTC

3121 aagttcagatcaaggtcaggaacagatggaacagctgaatatgggccaaacaggatatct 3180
TTCAAGTCTAGTTCCAGTCCTTGTCTACCTTGTCGACTTATACCCGGTTTGTCCTATAGA

3181 gtggtaagcagttcctgccccggctcagggccaagaacagatggaacagctgaatatggg 3240
CACCATTCGTCAAGGACGGGGCCGAGTCCCGGTTCTTGTCTACCTTGTCGACTTATACCC

3241 ccaaacaggatatctgtggtaagcagttcctgccccggctcagggccaagaacagatggt 3300
GGTTTGTCCTATAGACACCATTCGTCAAGGACGGGGCCGAGTCCCGGTTCTTGTCTACCA

XbaI 
|
3301 ccccagatgcggtccagccctcagcagtttctagagaaccatcagatgtttccagggtgc 3360
GGGGTCTACGCCAGGTCGGGAGTCGTCAAAGATCTCTTGGTAGTCTACAAAGGTCCCACG

3361 cccaaggacctgaaatgaccctgtgccttatttgaactaaccaatcagttcgcttctcgc 3420
GGGTTCCTGGACTTTACTGGGACACGGAATAAACTTGATTGGTTAGTCAAGCGAAGAGCG

3421 ttctgttcgcgcgcttctgctccccgagctcaataaaagagcccacaacccctcactcgg 3480
AAGACAAGCGCGCGAAGACGAGGGGCTCGAGTTATTTTCTCGGGTGTTGGGGAGTGAGCC

3481 ggcgccagtcctccgattgactgagtcgcccgggtacccgtgtatccaataaaccctctt 3540
CCGCGGTCAGGAGGCTAACTGACTCAGCGGGCCCATGGGCACATAGGTTATTTGGGAGAA

3541 gcagttgcatccgacttgtggtctcgctgttccttgggagggtctcctctgagtgattga 3600
CGTCAACGTAGGCTGAACACCAGAGCGACAAGGAACCCTCCCAGAGGAGACTCACTAACT

3601 ctacccgtcagcgggggtctttcatttgggggctcgtccgggatcgggagacccctgccc 3660
GATGGGCAGTCGCCCCCAGAAAGTAAACCCCCGAGCAGGCCCTAGCCCTCTGGGGACGGG

3661 agggaccaccgacccaccaccgggaggtaagctggctgcctcgcgcgtttcggtgatgac 3720
TCCCTGGTGGCTGGGTGGTGGCCCTCCATTCGACCGACGGAGCGCGCAAAGCCACTACTG

3721 ggtgaaaacctctgacacatgcagctcccggagacggtcacagcttgtctgtaagcggat 3780
CCACTTTTGGAGACTGTGTACGTCGAGGGCCTCTGCCAGTGTCGAACAGACATTCGCCTA

3781 gccgggagcagacaagcccgtcagggcgcgtcagcgggtgttggcgggtgtcggggcgca 3840
CGGCCCTCGTCTGTTCGGGCAGTCCCGCGCAGTCGCCCACAACCGCCCACAGCCCCGCGT

loxP other(3879,3912)<<< 
|
3841 gccatgacccagtcacgtagcgatagcggagtgtactgataacttcgtataatgtatgct 3900
CGGTACTGGGTCAGTGCATCGCTATCGCCTCACATGACTATTGAAGCATATTACATACGA

3901 atacgaagttattaggtctgaagaggagtttacgtccagccaagctagggccgcgatccg 3960
TATGCTTCAATAATCCAGACTTCTCCTCAAATGCAGGTCGGTTCGATCCCGGCGCTAGGC

loxP other(3972,4005)<<< 
|
3961 gaacccttaatataacttcgtataatgtatgctatacgaagttatcagtactggcttaac 4020
CTTGGGAATTATATTGAAGCATATTACATACGATATGCTTCAATAGTCATGACCGAATTG

4021 tatgcggcatcagagcagattgtactgagagtgcaccatatgcggtgtgaaataccgcac 4080
ATACGCCGTAGTCTCGTCTAACATGACTCTCACGTGGTATACGCCACACTTTATGGCGTG

4081 agatgcgtaaggagaaaataccgcatcaggcgctcttccgcttcctcgctcactgactcg 4140
TCTACGCATTCCTCTTTTATGGCGTAGTCCGCGAGAAGGCGAAGGAGCGAGTGACTGAGC

4141 ctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacgg 4200
GACGCGAGCCAGCAAGCCGACGCCGCTCGCCATAGTCGAGTGAGTTTCCGCCATTATGCC

4201 ttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaag 4260
AATAGGTGTCTTAGTCCCCTATTGCGTCCTTTCTTGTACACTCGTTTTCCGGTCGTTTTC

pBR322 origin(4281,4900)<<< 
|
4261 gccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgac 4320
CGGTCCTTGGCATTTTTCCGGCGCAACGACCGCAAAAAGGTATCCGAGGCGGGGGGACTG

4321 gagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaaga 4380
CTCGTAGTGTTTTTAGCTGCGAGTTCAGTCTCCACCGCTTTGGGCTGTCCTGATATTTCT

4381 taccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgctt 4440
ATGGTCCGCAAAGGGGGACCTTCGAGGGAGCACGCGAGAGGACAAGGCTGGGACGGCGAA

4441 accggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgc 4500
TGGCCTATGGACAGGCGGAAAGAGGGAAGCCCTTCGCACCGCGAAAGAGTATCGAGTGCG

4501 tgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccc 4560
ACATCCATAGAGTCAAGCCACATCCAGCAAGCGAGGTTCGACCCGACACACGTGCTTGGG

4561 cccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggta 4620
GGGCAAGTCGGGCTGGCGACGCGGAATAGGCCATTGATAGCAGAACTCAGGTTGGGCCAT

4621 agacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtat 4680
TCTGTGCTGAATAGCGGTGACCGTCGTCGGTGACCATTGTCCTAATCGTCTCGCTCCATA

4681 gtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggaca 4740
CATCCGCCACGATGTCTCAAGAACTTCACCACCGGATTGATGCCGATGTGATCTTCCTGT

4741 gtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctct 4800
CATAAACCATAGACGCGAGACGACTTCGGTCAATGGAAGCCTTTTTCTCAACCATCGAGA

4801 tgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagatt 4860
ACTAGGCCGTTTGTTTGGTGGCGACCATCGCCACCAAAAAAACAAACGTTCGTCGTCTAA

4861 acgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgct 4920
TGCGCGTCTTTTTTTCCTAGAGTTCTTCTAGGAAACTAGAAAAGATGCCCCAGACTGCGA

4921 cagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttc 4980
GTCACCTTGCTTTTGAGTGCAATTCCCTAAAACCAGTACTCTAATAGTTTTTCCTAGAAG

4981 acctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaa 5040
TGGATCTAGGAAAATTTAATTTTTACTTCAAAATTTAGTTAGATTTCATATATACTCATT

amp marker(5055,5915)<<< 
|
ORF_1 rf(5)(5055,5915)<<< 
|
5041 acttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtcta 5100
TGAACCAGACTGTCAATGGTTACGAATTAGTCACTCCGTGGATAGAGTCGCTAGACAGAT

5101 tttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggc 5160
AAAGCAAGTAGGTATCAACGGACTGAGGGGCAGCACATCTATTGATGCTATGCCCTCCCG

5161 ttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagat 5220
AATGGTAGACCGGGGTCACGACGTTACTATGGCGCTCTGGGTGCGAGTGGCCGAGGTCTA

5221 ttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaacttta 5280
AATAGTCGTTATTTGGTCGGTCGGCCTTCCCGGCTCGCGTCTTCACCAGGACGTTGAAAT

5281 tccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagtt 5340
AGGCGGAGGTAGGTCAGATAATTAACAACGGCCCTTCGATCTCATTCATCAAGCGGTCAA

5341 aatagtttgcgcaacgttgttgccattgctgcaggcatcgtggtgtcacgctcgtcgttt 5400
TTATCAAACGCGTTGCAACAACGGTAACGACGTCCGTAGCACCACAGTGCGAGCAGCAAA

5401 ggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatg 5460
CCATACCGAAGTAAGTCGAGGCCAAGGGTTGCTAGTTCCGCTCAATGTACTAGGGGGTAC

5461 ttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggcc 5520
AACACGTTTTTTCGCCAATCGAGGAAGCCAGGAGGCTAGCAACAGTCTTCATTCAACCGG

5521 gcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatcc 5580
CGTCACAATAGTGAGTACCAATACCGTCGTGACGTATTAAGAGAATGACAGTACGGTAGG

5581 gtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatg 5640
CATTCTACGAAAAGACACTGACCACTCATGAGTTGGTTCAGTAAGACTCTTATCACATAC

5641 cggcgaccgagttgctcttgcccggcgtcaacacgggataataccgcgccacatagcaga 5700
GCCGCTGGCTCAACGAGAACGGGCCGCAGTTGTGCCCTATTATGGCGCGGTGTATCGTCT

5701 actttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatctta 5760
TGAAATTTTCACGAGTAGTAACCTTTTGCAAGAAGCCCCGCTTTTGAGAGTTCCTAGAAT

5761 ccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatct 5820
GGCGACAACTCTAGGTCAAGCTACATTGGGTGAGCACGTGGGTTGACTAGAAGTCGTAGA

5821 tttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaag 5880
AAATGAAAGTGGTCGCAAAGACCCACTCGTTTTTGTCCTTCCGTTTTACGGCGTTTTTTC

5881 ggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattga 5940
CCTTATTCCCGCTGTGCCTTTACAACTTATGAGTATGAGAAGGAAAAAGTTATAATAACT

amp prom(5957,5985)<<< 
|
5941 agcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaat 6000
TCGTAAATAGTCCCAATAACAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTA

6001 aaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaacc 6060
TTTGTTTATCCCCAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTGCAGATTCTTTGG

6061 attattatcatgacattaacctataaaaataggcgtatcacgaggccctttcgtcttcaa 6120
TAATAATAGTACTGTAATTGGATATTTTTATCCGCATAGTGCTCCGGGAAAGCAGAAGTT

6121 gaattgctagcaattgctagcaattgctagcaattcataccagatcaccgaaaactgtcc 6180
CTTAACGATCGTTAACGATCGTTAACGATCGTTAAGTATGGTCTAGTGGCTTTTGACAGG

6181 tccaaatgtgtccccctcacactcccaaattcgcgggcttctgcctcttagaccactcta 6240
AGGTTTACACAGGGGGAGTGTGAGGGTTTAAGCGCCCGAAGACGGAGAATCTGGTGAGAT

6241 ccctattccccacactcaccggagccaaagccgcggcccttccgtttctttgct 6294
GGGATAAGGGGTGTGAGTGGCCTCGGTTTCGGCGCCGGGAAGGCAAAGAAACGA

                            Misc. Feature (6 total)
5'-LTR
Start: 1 End: 589 
Puro
Start: 1550 End: 2165
CMV
Start: 2338 End: 2868
MCS
Start: 2886 End: 2996
3'-LTR
Start: 3036 End: 3629
Amp
Start: 5058 End: 5916 (Complementary)