pRetro-Easy-His
| Vector Name | pRetro-Easy-His |
| VectorType | Retroviral Vector |
| Antibiotic Information | Ampicillin |
| Sequencing Primers | Sequencing Primer 5`---TAGGCGTGTACGGTGGGAGG---3` |
| Additional Information | No Additional Information |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 tttgaaagac cccacccgta ggtggcaagc tagcttaagt aacgccactt tgcaaggcat 60
61 ggaaaaatac ataactgaga atagaaaagt tcagatcaag gtcaggaaca aagaaacagc 120
121 tgaataccaa acaggatatc tgtggtaagc ggttcctgcc ccggctcagg gccaagaaca 180
181 gatgagacag ctgagtgatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg 240
241 ctcggggcca agaacagatg gtccccagat gcggtccagc cctcagcagt ttctagtgaa 300
301 tcatcagatg tttccagggt gccccaagga cctgaaaatg accctgtacc ttatttgaac 360
361 taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc cgctctccga gctcaataaa 420
421 agagcccaca acccctcact cggcgcgcca gtcttccgat agactgcgtc gcccgggtac 480
481 ccgtattccc aataaagcct cttgctgttt gcatccgaat cgtggtctcg ctgttccttg 540
541 ggagggtctc ctctgagtga ttgactaccc acgacggggg tctttcattt gggggctcgt 600
601 ccgggatttg gagacccctg cccagggacc accgacccac caccgggagg taagctggcc 660
661 agcaacttat ctgtgtctgt ccgattgtct agtgtctatg tttgatgtta tgcgcctgcg 720
721 tctgtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa ctgacgagtt 780
781 ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc gtttttgtgg 840
841 cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg tggttctggt 900
901 aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt cggtttggaa 960
961 ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct 1020
1021 gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc ccttaagttt 1080
1081 gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa 1140
1141 gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg tcggatggcc 1200
1201 gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg tcttttcacc 1260
1261 tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag ccttggcttt 1320
1321 tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc ctcttcctcc 1380
1381 atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta 1440
1441 tccagccctc actccttctc taggcgccgg aattgatccc ccgggctgca ggtcggccgc 1500
1501 cacgaccggt gccgccacca tcccctgacc cacgcccctg acccctcaca aggagacgac 1560
1561 cttccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtcccccggg 1620
1621 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgacc 1680
1681 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc 1740
1741 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc 1800
1801 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga 1860
1861 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca 1920
1921 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc 1980
1981 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct 2040
2041 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca 2100
2101 ccgccgacgt cgagtgcccg aaggaccgcg cgacctggtg catgacccgc aagcccggtg 2160
2161 cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga ccccatggct 2220
2221 ccgaccgaag ccgacccggg cggccccgcc gaccccgcac ccgcccccga ggcccaccga 2280
2281 ctctagtaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc 2340
2341 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg 2400
2401 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa 2460
2461 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca 2520
2521 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac 2580
2581 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc 2640
2641 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga 2700
2701 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg 2760
2761 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta 2820
2821 cggtgggagg tctatataag cagagctggt ttagtgaacc gtcagatccg ctagcgctac 2880
2881 cggactcaga tctgggcccg gatccgaatt caagcttgtt aacagcggcc gccatttaaa 2940
2941 tctcgagcat catcaccatc accattgaat cgataaaata aaagatttta tttagtctcc 3000
3001 agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg caagctagct taagtaacgc 3060
3061 cattttgcaa ggcatggaaa aatacataac tgagaataga gaagttcaga tcaaggtcag 3120
3121 gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc 3180
3181 ccggctcagg gccaagaaca gatggaacag ctgaatatgg gccaaacagg atatctgtgg 3240
3241 taagcagttc ctgccccggc tcagggccaa gaacagatgg tccccagatg cggtccagcc 3300
3301 ctcagcagtt tctagagaac catcagatgt ttccagggtg ccccaaggac ctgaaatgac 3360
3361 cctgtgcctt atttgaacta accaatcagt tcgcttctcg cttctgttcg cgcgcttctg 3420
3421 ctccccgagc tcaataaaag agcccacaac ccctcactcg gggcgccagt cctccgattg 3480
3481 actgagtcgc ccgggtaccc gtgtatccaa taaaccctct tgcagttgca tccgacttgt 3540
3541 ggtctcgctg ttccttggga gggtctcctc tgagtgattg actacccgtc agcgggggtc 3600
3601 tttcatttgg gggctcgtcc gggatcggga gacccctgcc cagggaccac cgacccacca 3660
3661 ccgggaggta agctggctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca 3720
3721 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc 3780
3781 gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta 3840
3841 gcgatagcgg agtgtactga taacttcgta taatgtatgc tatacgaagt tattaggtct 3900
3901 gaagaggagt ttacgtccag ccaagctagg gccgcgatcc ggaaccctta atataacttc 3960
3961 gtataatgta tgctatacga agttatcagt actggcttaa ctatgcggca tcagagcaga 4020
4021 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat 4080
4081 accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 4140
4141 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg 4200
4201 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg 4260
4261 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 4320
4321 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 4380
4381 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 4440
4441 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg 4500
4501 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 4560
4561 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 4620
4621 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 4680
4681 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc 4740
4741 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 4800
4801 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 4860
4861 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 4920
4921 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt 4980
4981 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc 5040
5041 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg 5100
5101 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg 5160
5161 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc 5220
5221 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta 5280
5281 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg 5340
5341 ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct 5400
5401 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta 5460
5461 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg 5520
5521 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga 5580
5581 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt 5640
5641 gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca 5700
5701 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt 5760
5761 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt 5820
5821 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga 5880
5881 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt 5940
5941 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc 6000
6001 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa 6060
6061 cctataaaaa taggcgtatc acgaggccct ttcgtcttca agaattgcta gcaattgcta 6120
6121 gcaattgcta gcaattcata ccagatcacc gaaaactgtc ctccaaatgt gtccccctca 6180
6181 cactcccaaa ttcgcgggct tctgcctctt agaccactct accctattcc ccacactcac 6240
6241 cggagccaaa gccgcggccc ttccgtttct ttgct 6275
LOCUS dna 6275 bp
FEATURES Location/Qualifiers
Other Gene 659..1468
/gene="psi_plus_pack other"
ORF 1449..2216
/sequence="ORF_1 rf(4)"
Marker 1566..2165
/gene="puro marker"
misc_binding 1674..1679
/dbxref="REBASE:AccI"
misc_binding 1895..1900
/dbxref="REBASE:StuI"
Promoter 2787..2868
/gene="CMV prom"
misc_binding 2888..2893
/dbxref="REBASE:BglII"
misc_binding 2894..2899
/dbxref="REBASE:ApaI"
misc_binding 2900..2905
/dbxref="REBASE:BamHI"
misc_binding 2906..2911
/dbxref="REBASE:EcoRI"
misc_binding 2912..2917
/dbxref="REBASE:HindIII"
misc_binding 2918..2923
/dbxref="REBASE:HpaI"
misc_binding 2925..2932
/dbxref="REBASE:NotI"
misc_binding 2942..2947
/dbxref="REBASE:XhoI"
Tag 2948..2965
/gene="6xHis tag"
misc_binding 2969..2974
/dbxref="REBASE:ClaI"
Other Gene 3056..3607
/gene="5_LTR2 other"
misc_binding 3311..3316
/dbxref="REBASE:XbaI"
Other Gene 3860..3893
/gene="loxP other"
Other Gene 3953..3986
/gene="loxP other"
Rep_Origin 4262..4881
/gene="pBR322 origin"
Marker 5036..5896
/gene="amp marker"
ORF 5036..5896
/sequence="ORF_2 rf(5)"
Promoter 5938..5966
/gene="amp prom"
BASE COUNT 1445 a 1808 c 1597 g 1425 t 0 others
ORIGIN
1 tttgaaagac cccacccgta ggtggcaagc tagcttaagt aacgccactt tgcaaggcat
61 ggaaaaatac ataactgaga atagaaaagt tcagatcaag gtcaggaaca aagaaacagc
121 tgaataccaa acaggatatc tgtggtaagc ggttcctgcc ccggctcagg gccaagaaca
181 gatgagacag ctgagtgatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg
241 ctcggggcca agaacagatg gtccccagat gcggtccagc cctcagcagt ttctagtgaa
301 tcatcagatg tttccagggt gccccaagga cctgaaaatg accctgtacc ttatttgaac
361 taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc cgctctccga gctcaataaa
421 agagcccaca acccctcact cggcgcgcca gtcttccgat agactgcgtc gcccgggtac
481 ccgtattccc aataaagcct cttgctgttt gcatccgaat cgtggtctcg ctgttccttg
541 ggagggtctc ctctgagtga ttgactaccc acgacggggg tctttcattt gggggctcgt
601 ccgggatttg gagacccctg cccagggacc accgacccac caccgggagg taagctggcc
661 agcaacttat ctgtgtctgt ccgattgtct agtgtctatg tttgatgtta tgcgcctgcg
721 tctgtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa ctgacgagtt
781 ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc gtttttgtgg
841 cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg tggttctggt
901 aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt cggtttggaa
961 ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct
1021 gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc ccttaagttt
1081 gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa
1141 gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg tcggatggcc
1201 gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg tcttttcacc
1261 tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag ccttggcttt
1321 tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc ctcttcctcc
1381 atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta
1441 tccagccctc actccttctc taggcgccgg aattgatccc ccgggctgca ggtcggccgc
1501 cacgaccggt gccgccacca tcccctgacc cacgcccctg acccctcaca aggagacgac
1561 cttccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtcccccggg
1621 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgacc
1681 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc
1741 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc
1801 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga
1861 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca
1921 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc
1981 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct
2041 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca
2101 ccgccgacgt cgagtgcccg aaggaccgcg cgacctggtg catgacccgc aagcccggtg
2161 cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga ccccatggct
2221 ccgaccgaag ccgacccggg cggccccgcc gaccccgcac ccgcccccga ggcccaccga
2281 ctctagtaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
2341 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
2401 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
2461 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
2521 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
2581 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
2641 atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga
2701 tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg
2761 gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta
2821 cggtgggagg tctatataag cagagctggt ttagtgaacc gtcagatccg ctagcgctac
2881 cggactcaga tctgggcccg gatccgaatt caagcttgtt aacagcggcc gccatttaaa
2941 tctcgagcat catcaccatc accattgaat cgataaaata aaagatttta tttagtctcc
3001 agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg caagctagct taagtaacgc
3061 cattttgcaa ggcatggaaa aatacataac tgagaataga gaagttcaga tcaaggtcag
3121 gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc
3181 ccggctcagg gccaagaaca gatggaacag ctgaatatgg gccaaacagg atatctgtgg
3241 taagcagttc ctgccccggc tcagggccaa gaacagatgg tccccagatg cggtccagcc
3301 ctcagcagtt tctagagaac catcagatgt ttccagggtg ccccaaggac ctgaaatgac
3361 cctgtgcctt atttgaacta accaatcagt tcgcttctcg cttctgttcg cgcgcttctg
3421 ctccccgagc tcaataaaag agcccacaac ccctcactcg gggcgccagt cctccgattg
3481 actgagtcgc ccgggtaccc gtgtatccaa taaaccctct tgcagttgca tccgacttgt
3541 ggtctcgctg ttccttggga gggtctcctc tgagtgattg actacccgtc agcgggggtc
3601 tttcatttgg gggctcgtcc gggatcggga gacccctgcc cagggaccac cgacccacca
3661 ccgggaggta agctggctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
3721 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
3781 gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta
3841 gcgatagcgg agtgtactga taacttcgta taatgtatgc tatacgaagt tattaggtct
3901 gaagaggagt ttacgtccag ccaagctagg gccgcgatcc ggaaccctta atataacttc
3961 gtataatgta tgctatacga agttatcagt actggcttaa ctatgcggca tcagagcaga
4021 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
4081 accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
4141 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
4201 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
4261 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
4321 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
4381 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
4441 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
4501 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
4561 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
4621 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
4681 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
4741 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
4801 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
4861 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
4921 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
4981 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
5041 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
5101 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
5161 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
5221 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
5281 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
5341 ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
5401 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
5461 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
5521 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
5581 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
5641 gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
5701 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
5761 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
5821 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
5881 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
5941 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
6001 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
6061 cctataaaaa taggcgtatc acgaggccct ttcgtcttca agaattgcta gcaattgcta
6121 gcaattgcta gcaattcata ccagatcacc gaaaactgtc ctccaaatgt gtccccctca
6181 cactcccaaa ttcgcgggct tctgcctctt agaccactct accctattcc ccacactcac
6241 cggagccaaa gccgcggccc ttccgtttct ttgct
//
***Note: The text map shown below is representative of the parental
vector only and does not include the gene of interest. The sequence of the gene
can be found through the accession number link on the product page.
1 tttgaaagaccccacccgtaggtggcaagctagcttaagtaacgccactttgcaaggcat 60
AAACTTTCTGGGGTGGGCATCCACCGTTCGATCGAATTCATTGCGGTGAAACGTTCCGTA
61 ggaaaaatacataactgagaatagaaaagttcagatcaaggtcaggaacaaagaaacagc 120
CCTTTTTATGTATTGACTCTTATCTTTTCAAGTCTAGTTCCAGTCCTTGTTTCTTTGTCG
121 tgaataccaaacaggatatctgtggtaagcggttcctgccccggctcagggccaagaaca 180
ACTTATGGTTTGTCCTATAGACACCATTCGCCAAGGACGGGGCCGAGTCCCGGTTCTTGT
181 gatgagacagctgagtgatgggccaaacaggatatctgtggtaagcagttcctgccccgg 240
CTACTCTGTCGACTCACTACCCGGTTTGTCCTATAGACACCATTCGTCAAGGACGGGGCC
241 ctcggggccaagaacagatggtccccagatgcggtccagccctcagcagtttctagtgaa 300
GAGCCCCGGTTCTTGTCTACCAGGGGTCTACGCCAGGTCGGGAGTCGTCAAAGATCACTT
301 tcatcagatgtttccagggtgccccaaggacctgaaaatgaccctgtaccttatttgaac 360
AGTAGTCTACAAAGGTCCCACGGGGTTCCTGGACTTTTACTGGGACATGGAATAAACTTG
361 taaccaatcagttcgcttctcgcttctgttcgcgcgcttccgctctccgagctcaataaa 420
ATTGGTTAGTCAAGCGAAGAGCGAAGACAAGCGCGCGAAGGCGAGAGGCTCGAGTTATTT
421 agagcccacaacccctcactcggcgcgccagtcttccgatagactgcgtcgcccgggtac 480
TCTCGGGTGTTGGGGAGTGAGCCGCGCGGTCAGAAGGCTATCTGACGCAGCGGGCCCATG
481 ccgtattcccaataaagcctcttgctgtttgcatccgaatcgtggtctcgctgttccttg 540
GGCATAAGGGTTATTTCGGAGAACGACAAACGTAGGCTTAGCACCAGAGCGACAAGGAAC
541 ggagggtctcctctgagtgattgactacccacgacgggggtctttcatttgggggctcgt 600
CCTCCCAGAGGAGACTCACTAACTGATGGGTGCTGCCCCCAGAAAGTAAACCCCCGAGCA
psi_plus_pack other(659,1468)>>>
|
601 ccgggatttggagacccctgcccagggaccaccgacccaccaccgggaggtaagctggcc 660
GGCCCTAAACCTCTGGGGACGGGTCCCTGGTGGCTGGGTGGTGGCCCTCCATTCGACCGG
661 agcaacttatctgtgtctgtccgattgtctagtgtctatgtttgatgttatgcgcctgcg 720
TCGTTGAATAGACACAGACAGGCTAACAGATCACAGATACAAACTACAATACGCGGACGC
721 tctgtactagttagctaactagctctgtatctggcggacccgtggtggaactgacgagtt 780
AGACATGATCAATCGATTGATCGAGACATAGACCGCCTGGGCACCACCTTGACTGCTCAA
781 ctgaacacccggccgcaaccctgggagacgtcccagggactttgggggccgtttttgtgg 840
GACTTGTGGGCCGGCGTTGGGACCCTCTGCAGGGTCCCTGAAACCCCCGGCAAAAACACC
841 cccgacctgaggaagggagtcgatgtggaatccgaccccgtcaggatatgtggttctggt 900
GGGCTGGACTCCTTCCCTCAGCTACACCTTAGGCTGGGGCAGTCCTATACACCAAGACCA
901 aggagacgagaacctaaaacagttcccgcctccgtctgaatttttgctttcggtttggaa 960
TCCTCTGCTCTTGGATTTTGTCAAGGGCGGAGGCAGACTTAAAAACGAAAGCCAAACCTT
961 ccgaagccgcgcgtcttgtctgctgcagcgctgcagcatcgttctgtgttgtctctgtct 1020
GGCTTCGGCGCGCAGAACAGACGACGTCGCGACGTCGTAGCAAGACACAACAGAGACAGA
1021 gactgtgtttctgtatttgtctgaaaattagggccagactgttaccactcccttaagttt 1080
CTGACACAAAGACATAAACAGACTTTTAATCCCGGTCTGACAATGGTGAGGGAATTCAAA
1081 gaccttaggtcactggaaagatgtcgagcggatcgctcacaaccagtcggtagatgtcaa 1140
CTGGAATCCAGTGACCTTTCTACAGCTCGCCTAGCGAGTGTTGGTCAGCCATCTACAGTT
1141 gaagagacgttgggttaccttctgctctgcagaatggccaacctttaacgtcggatggcc 1200
CTTCTCTGCAACCCAATGGAAGACGAGACGTCTTACCGGTTGGAAATTGCAGCCTACCGG
1201 gcgagacggcacctttaaccgagacctcatcacccaggttaagatcaaggtcttttcacc 1260
CGCTCTGCCGTGGAAATTGGCTCTGGAGTAGTGGGTCCAATTCTAGTTCCAGAAAAGTGG
1261 tggcccgcatggacacccagaccaggtcccctacatcgtgacctgggaagccttggcttt 1320
ACCGGGCGTACCTGTGGGTCTGGTCCAGGGGATGTAGCACTGGACCCTTCGGAACCGAAA
1321 tgacccccctccctgggtcaagccctttgtacaccctaagcctccgcctcctcttcctcc 1380
ACTGGGGGGAGGGACCCAGTTCGGGAAACATGTGGGATTCGGAGGCGGAGGAGAAGGAGG
1381 atccgccccgtctctcccccttgaacctcctcgttcgaccccgcctcgatcctcccttta 1440
TAGGCGGGGCAGAGAGGGGGAACTTGGAGGAGCAAGCTGGGGCGGAGCTAGGAGGGAAAT
ORF_1 rf(4)(1449,2216)<<<
|
1441 tccagccctcactccttctctaggcgccggaattgatcccccgggctgcaggtcggccgc 1500
AGGTCGGGAGTGAGGAAGAGATCCGCGGCCTTAACTAGGGGGCCCGACGTCCAGCCGGCG
1501 cacgaccggtgccgccaccatcccctgacccacgcccctgacccctcacaaggagacgac 1560
GTGCTGGCCACGGCGGTGGTAGGGGACTGGGTGCGGGGACTGGGGAGTGTTCCTCTGCTG
puro marker(1566,2165)>>>
|
1561 cttccatgaccgagtacaagcccacggtgcgcctcgccacccgcgacgacgtcccccggg 1620
GAAGGTACTGGCTCATGTTCGGGTGCCACGCGGAGCGGTGGGCGCTGCTGCAGGGGGCCC
AccI
|
1621 ccgtacgcaccctcgccgccgcgttcgccgactaccccgccacgcgccacaccgtcgacc 1680
GGCATGCGTGGGAGCGGCGGCGCAAGCGGCTGATGGGGCGGTGCGCGGTGTGGCAGCTGG
1681 cggaccgccacatcgagcgggtcaccgagctgcaagaactcttcctcacgcgcgtcgggc 1740
GCCTGGCGGTGTAGCTCGCCCAGTGGCTCGACGTTCTTGAGAAGGAGTGCGCGCAGCCCG
1741 tcgacatcggcaaggtgtgggtcgcggacgacggcgccgcggtggcggtctggaccacgc 1800
AGCTGTAGCCGTTCCACACCCAGCGCCTGCTGCCGCGGCGCCACCGCCAGACCTGGTGCG
1801 cggagagcgtcgaagcgggggcggtgttcgccgagatcggcccgcgcatggccgagttga 1860
GCCTCTCGCAGCTTCGCCCCCGCCACAAGCGGCTCTAGCCGGGCGCGTACCGGCTCAACT
StuI
|
1861 gcggttcccggctggccgcgcagcaacagatggaaggcctcctggcgccgcaccggccca 1920
CGCCAAGGGCCGACCGGCGCGTCGTTGTCTACCTTCCGGAGGACCGCGGCGTGGCCGGGT
1921 aggagcccgcgtggttcctggccaccgtcggcgtctcgcccgaccaccagggcaagggtc 1980
TCCTCGGGCGCACCAAGGACCGGTGGCAGCCGCAGAGCGGGCTGGTGGTCCCGTTCCCAG
1981 tgggcagcgccgtcgtgctccccggagtggaggcggccgagcgcgccggggtgcccgcct 2040
ACCCGTCGCGGCAGCACGAGGGGCCTCACCTCCGCCGGCTCGCGCGGCCCCACGGGCGGA
2041 tcctggagacctccgcgccccgcaacctccccttctacgagcggctcggcttcaccgtca 2100
AGGACCTCTGGAGGCGCGGGGCGTTGGAGGGGAAGATGCTCGCCGAGCCGAAGTGGCAGT
2101 ccgccgacgtcgagtgcccgaaggaccgcgcgacctggtgcatgacccgcaagcccggtg 2160
GGCGGCTGCAGCTCACGGGCTTCCTGGCGCGCTGGACCACGTACTGGGCGTTCGGGCCAC
2161 cctgacgcccgccccacgacccgcagcgcccgaccgaaaggagcgcacgaccccatggct 2220
GGACTGCGGGCGGGGTGCTGGGCGTCGCGGGCTGGCTTTCCTCGCGTGCTGGGGTACCGA
2221 ccgaccgaagccgacccgggcggccccgccgaccccgcacccgcccccgaggcccaccga 2280
GGCTGGCTTCGGCTGGGCCCGCCGGGGCGGCTGGGGCGTGGGCGGGGGCTCCGGGTGGCT
2281 ctctagtaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgc 2340
GAGATCATTATCATTAGTTAATGCCCCAGTAATCAAGTATCGGGTATATACCTCAAGGCG
2341 gttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattg 2400
CAATGTATTGAATGCCATTTACCGGGCGGACCGACTGGCGGGTTGCTGGGGGCGGGTAAC
2401 acgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaa 2460
TGCAGTTATTACTGCATACAAGGGTATCATTGCGGTTATCCCTGAAAGGTAACTGCAGTT
2461 tgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgcca 2520
ACCCACCTCATAAATGCCATTTGACGGGTGAACCGTCATGTAGTTCACATAGTATACGGT
2521 agtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtac 2580
TCATGCGGGGGATAACTGCAGTTACTGCCATTTACCGGGCGGACCGTAATACGGGTCATG
2581 atgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattacc 2640
TACTGGAATACCCTGAAAGGATGAACCGTCATGTAGATGCATAATCAGTAGCGATAATGG
2641 atggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacgggga 2700
TACCACTACGCCAAAACCGTCATGTAGTTACCCGCACCTATCGCCAAACTGAGTGCCCCT
2701 tttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgg 2760
AAAGGTTCAGAGGTGGGGTAACTGCAGTTACCCTCAAACAAAACCGTGGTTTTAGTTGCC
CMV prom(2787,2868)>>>
|
2761 gactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgta 2820
CTGAAAGGTTTTACAGCATTGTTGAGGCGGGGTAACTGCGTTTACCCGCCATCCGCACAT
2821 cggtgggaggtctatataagcagagctggtttagtgaaccgtcagatccgctagcgctac 2880
GCCACCCTCCAGATATATTCGTCTCGACCAAATCACTTGGCAGTCTAGGCGATCGCGATG
BamHI
|
BglII ApaI EcoRI HindIII HpaI NotI
| | | | | | |
2881 cggactcagatctgggcccggatccgaattcaagcttgttaacagcggccgccatttaaa 2940
GCCTGAGTCTAGACCCGGGCCTAGGCTTAAGTTCGAACAATTGTCGCCGGCGGTAAATTT
ClaI
|
XhoI 6xHis tag(2948,2965)>>>
| | |
2941 tctcgagcatcatcaccatcaccattgaatcgataaaataaaagattttatttagtctcc 3000
AGAGCTCGTAGTAGTGGTAGTGGTAACTTAGCTATTTTATTTTCTAAAATAAATCAGAGG
5_LTR2 other(3056,3607)>>>
|
3001 agaaaaaggggggaatgaaagaccccacctgtaggtttggcaagctagcttaagtaacgc 3060
TCTTTTTCCCCCCTTACTTTCTGGGGTGGACATCCAAACCGTTCGATCGAATTCATTGCG
3061 cattttgcaaggcatggaaaaatacataactgagaatagagaagttcagatcaaggtcag 3120
GTAAAACGTTCCGTACCTTTTTATGTATTGACTCTTATCTCTTCAAGTCTAGTTCCAGTC
3121 gaacagatggaacagctgaatatgggccaaacaggatatctgtggtaagcagttcctgcc 3180
CTTGTCTACCTTGTCGACTTATACCCGGTTTGTCCTATAGACACCATTCGTCAAGGACGG
3181 ccggctcagggccaagaacagatggaacagctgaatatgggccaaacaggatatctgtgg 3240
GGCCGAGTCCCGGTTCTTGTCTACCTTGTCGACTTATACCCGGTTTGTCCTATAGACACC
3241 taagcagttcctgccccggctcagggccaagaacagatggtccccagatgcggtccagcc 3300
ATTCGTCAAGGACGGGGCCGAGTCCCGGTTCTTGTCTACCAGGGGTCTACGCCAGGTCGG
XbaI
|
3301 ctcagcagtttctagagaaccatcagatgtttccagggtgccccaaggacctgaaatgac 3360
GAGTCGTCAAAGATCTCTTGGTAGTCTACAAAGGTCCCACGGGGTTCCTGGACTTTACTG
3361 cctgtgccttatttgaactaaccaatcagttcgcttctcgcttctgttcgcgcgcttctg 3420
GGACACGGAATAAACTTGATTGGTTAGTCAAGCGAAGAGCGAAGACAAGCGCGCGAAGAC
3421 ctccccgagctcaataaaagagcccacaacccctcactcggggcgccagtcctccgattg 3480
GAGGGGCTCGAGTTATTTTCTCGGGTGTTGGGGAGTGAGCCCCGCGGTCAGGAGGCTAAC
3481 actgagtcgcccgggtacccgtgtatccaataaaccctcttgcagttgcatccgacttgt 3540
TGACTCAGCGGGCCCATGGGCACATAGGTTATTTGGGAGAACGTCAACGTAGGCTGAACA
3541 ggtctcgctgttccttgggagggtctcctctgagtgattgactacccgtcagcgggggtc 3600
CCAGAGCGACAAGGAACCCTCCCAGAGGAGACTCACTAACTGATGGGCAGTCGCCCCCAG
3601 tttcatttgggggctcgtccgggatcgggagacccctgcccagggaccaccgacccacca 3660
AAAGTAAACCCCCGAGCAGGCCCTAGCCCTCTGGGGACGGGTCCCTGGTGGCTGGGTGGT
3661 ccgggaggtaagctggctgcctcgcgcgtttcggtgatgacggtgaaaacctctgacaca 3720
GGCCCTCCATTCGACCGACGGAGCGCGCAAAGCCACTACTGCCACTTTTGGAGACTGTGT
3721 tgcagctcccggagacggtcacagcttgtctgtaagcggatgccgggagcagacaagccc 3780
ACGTCGAGGGCCTCTGCCAGTGTCGAACAGACATTCGCCTACGGCCCTCGTCTGTTCGGG
3781 gtcagggcgcgtcagcgggtgttggcgggtgtcggggcgcagccatgacccagtcacgta 3840
CAGTCCCGCGCAGTCGCCCACAACCGCCCACAGCCCCGCGTCGGTACTGGGTCAGTGCAT
loxP other(3860,3893)<<<
|
3841 gcgatagcggagtgtactgataacttcgtataatgtatgctatacgaagttattaggtct 3900
CGCTATCGCCTCACATGACTATTGAAGCATATTACATACGATATGCTTCAATAATCCAGA
loxP other(3953,3986)<<<
|
3901 gaagaggagtttacgtccagccaagctagggccgcgatccggaacccttaatataacttc 3960
CTTCTCCTCAAATGCAGGTCGGTTCGATCCCGGCGCTAGGCCTTGGGAATTATATTGAAG
3961 gtataatgtatgctatacgaagttatcagtactggcttaactatgcggcatcagagcaga 4020
CATATTACATACGATATGCTTCAATAGTCATGACCGAATTGATACGCCGTAGTCTCGTCT
4021 ttgtactgagagtgcaccatatgcggtgtgaaataccgcacagatgcgtaaggagaaaat 4080
AACATGACTCTCACGTGGTATACGCCACACTTTATGGCGTGTCTACGCATTCCTCTTTTA
4081 accgcatcaggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggc 4140
TGGCGTAGTCCGCGAGAAGGCGAAGGAGCGAGTGACTGAGCGACGCGAGCCAGCAAGCCG
4141 tgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcagggg 4200
ACGCCGCTCGCCATAGTCGAGTGAGTTTCCGCCATTATGCCAATAGGTGTCTTAGTCCCC
4201 ataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaagg 4260
TATTGCGTCCTTTCTTGTACACTCGTTTTCCGGTCGTTTTCCGGTCCTTGGCATTTTTCC
pBR322 origin(4262,4881)<<<
|
4261 ccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgac 4320
GGCGCAACGACCGCAAAAAGGTATCCGAGGCGGGGGGACTGCTCGTAGTGTTTTTAGCTG
4321 gctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctg 4380
CGAGTTCAGTCTCCACCGCTTTGGGCTGTCCTGATATTTCTATGGTCCGCAAAGGGGGAC
4381 gaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcct 4440
CTTCGAGGGAGCACGCGAGAGGACAAGGCTGGGACGGCGAATGGCCTATGGACAGGCGGA
4441 ttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcgg 4500
AAGAGGGAAGCCCTTCGCACCGCGAAAGAGTATCGAGTGCGACATCCATAGAGTCAAGCC
4501 tgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgct 4560
ACATCCAGCAAGCGAGGTTCGACCCGACACACGTGCTTGGGGGGCAAGTCGGGCTGGCGA
4561 gcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccac 4620
CGCGGAATAGGCCATTGATAGCAGAACTCAGGTTGGGCCATTCTGTGCTGAATAGCGGTG
4621 tggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagt 4680
ACCGTCGTCGGTGACCATTGTCCTAATCGTCTCGCTCCATACATCCGCCACGATGTCTCA
4681 tcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctc 4740
AGAACTTCACCACCGGATTGATGCCGATGTGATCTTCCTGTCATAAACCATAGACGCGAG
4741 tgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaacca 4800
ACGACTTCGGTCAATGGAAGCCTTTTTCTCAACCATCGAGAACTAGGCCGTTTGTTTGGT
4801 ccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggat 4860
GGCGACCATCGCCACCAAAAAAACAAACGTTCGTCGTCTAATGCGCGTCTTTTTTTCCTA
4861 ctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcac 4920
GAGTTCTTCTAGGAAACTAGAAAAGATGCCCCAGACTGCGAGTCACCTTGCTTTTGAGTG
4921 gttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaatt 4980
CAATTCCCTAAAACCAGTACTCTAATAGTTTTTCCTAGAAGTGGATCTAGGAAAATTTAA
ORF_2 rf(5)(5036,5896)<<<
|
amp marker(5036,5896)<<<
|
4981 aaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttacc 5040
TTTTTACTTCAAAATTTAGTTAGATTTCATATATACTCATTTGAACCAGACTGTCAATGG
5041 aatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttg 5100
TTACGAATTAGTCACTCCGTGGATAGAGTCGCTAGACAGATAAAGCAAGTAGGTATCAAC
5101 cctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtg 5160
GGACTGAGGGGCAGCACATCTATTGATGCTATGCCCTCCCGAATGGTAGACCGGGGTCAC
5161 ctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagc 5220
GACGTTACTATGGCGCTCTGGGTGCGAGTGGCCGAGGTCTAAATAGTCGTTATTTGGTCG
5221 cagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtcta 5280
GTCGGCCTTCCCGGCTCGCGTCTTCACCAGGACGTTGAAATAGGCGGAGGTAGGTCAGAT
5281 ttaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttg 5340
AATTAACAACGGCCCTTCGATCTCATTCATCAAGCGGTCAATTATCAAACGCGTTGCAAC
5341 ttgccattgctgcaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagct 5400
AACGGTAACGACGTCCGTAGCACCACAGTGCGAGCAGCAAACCATACCGAAGTAAGTCGA
5401 ccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggtta 5460
GGCCAAGGGTTGCTAGTTCCGCTCAATGTACTAGGGGGTACAACACGTTTTTTCGCCAAT
5461 gctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatgg 5520
CGAGGAAGCCAGGAGGCTAGCAACAGTCTTCATTCAACCGGCGTCACAATAGTGAGTACC
5521 ttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtga 5580
AATACCGTCGTGACGTATTAAGAGAATGACAGTACGGTAGGCATTCTACGAAAAGACACT
5581 ctggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctctt 5640
GACCACTCATGAGTTGGTTCAGTAAGACTCTTATCACATACGCCGCTGGCTCAACGAGAA
5641 gcccggcgtcaacacgggataataccgcgccacatagcagaactttaaaagtgctcatca 5700
CGGGCCGCAGTTGTGCCCTATTATGGCGCGGTGTATCGTCTTGAAATTTTCACGAGTAGT
5701 ttggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagtt 5760
AACCTTTTGCAAGAAGCCCCGCTTTTGAGAGTTCCTAGAATGGCGACAACTCTAGGTCAA
5761 cgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgttt 5820
GCTACATTGGGTGAGCACGTGGGTTGACTAGAAGTCGTAGAAAATGAAAGTGGTCGCAAA
5821 ctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacgga 5880
GACCCACTCGTTTTTGTCCTTCCGTTTTACGGCGTTTTTTCCCTTATTCCCGCTGTGCCT
amp prom(5938,5966)<<<
|
5881 aatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttatt 5940
TTACAACTTATGAGTATGAGAAGGAAAAAGTTATAATAACTTCGTAAATAGTCCCAATAA
5941 gtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgc 6000
CAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTATCCCCAAGGCG
6001 gcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaa 6060
CGTGTAAAGGGGCTTTTCACGGTGGACTGCAGATTCTTTGGTAATAATAGTACTGTAATT
6061 cctataaaaataggcgtatcacgaggccctttcgtcttcaagaattgctagcaattgcta 6120
GGATATTTTTATCCGCATAGTGCTCCGGGAAAGCAGAAGTTCTTAACGATCGTTAACGAT
6121 gcaattgctagcaattcataccagatcaccgaaaactgtcctccaaatgtgtccccctca 6180
CGTTAACGATCGTTAAGTATGGTCTAGTGGCTTTTGACAGGAGGTTTACACAGGGGGAGT
6181 cactcccaaattcgcgggcttctgcctcttagaccactctaccctattccccacactcac 6240
GTGAGGGTTTAAGCGCCCGAAGACGGAGAATCTGGTGAGATGGGATAAGGGGTGTGAGTG
6241 cggagccaaagccgcggcccttccgtttctttgct 6275
GCCTCGGTTTCGGCGCCGGGAAGGCAAAGAAACGA
Misc. Feature (5 total)
5'-LTR
Start: 1 End: 589
Puro
Start: 1550 End: 2165
CMV
Start: 2338 End: 2840
3'-LTR
Start: 3017 End: 3610
Amp
Start: 5039 End: 5897 (Complementary)