pRetro-Easy-HA

Vector Name pRetro-Easy-HA
VectorType Retroviral vector
Antibiotic Information Ampicillin
Sequencing Primers Sequencing Primer
5`---CCCTTGAACCTCCTCGTTCGACC---3`
Additional Information No Additional Information
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector only and does not include the gene of interest. The sequence of the gene can be found through the accession number link on the product page. The restriction sites within the cloning region may differ from the provided sequence.
1       tttgaaagac cccacccgta ggtggcaagc tagcttaagt aacgccactt tgcaaggcat  60
61 ggaaaaatac ataactgaga atagaaaagt tcagatcaag gtcaggaaca aagaaacagc 120
121 tgaataccaa acaggatatc tgtggtaagc ggttcctgcc ccggctcagg gccaagaaca 180
181 gatgagacag ctgagtgatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg 240
241 ctcggggcca agaacagatg gtccccagat gcggtccagc cctcagcagt ttctagtgaa 300
301 tcatcagatg tttccagggt gccccaagga cctgaaaatg accctgtacc ttatttgaac 360
361 taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc cgctctccga gctcaataaa 420
421 agagcccaca acccctcact cggcgcgcca gtcttccgat agactgcgtc gcccgggtac 480
481 ccgtattccc aataaagcct cttgctgttt gcatccgaat cgtggtctcg ctgttccttg 540
541 ggagggtctc ctctgagtga ttgactaccc acgacggggg tctttcattt gggggctcgt 600
601 ccgggatttg gagacccctg cccagggacc accgacccac caccgggagg taagctggcc 660
661 agcaacttat ctgtgtctgt ccgattgtct agtgtctatg tttgatgtta tgcgcctgcg 720
721 tctgtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa ctgacgagtt 780
781 ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc gtttttgtgg 840
841 cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg tggttctggt 900
901 aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt cggtttggaa 960
961 ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct 1020
1021 gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc ccttaagttt 1080
1081 gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa 1140
1141 gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg tcggatggcc 1200
1201 gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg tcttttcacc 1260
1261 tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag ccttggcttt 1320
1321 tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc ctcttcctcc 1380
1381 atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta 1440
1441 tccagccctc actccttctc taggcgccgg aattcgggcc cagatctatc gatgttaacg 1500
1501 cggccgcgtc gactacgtaa tttaaattac ccatacgacg tcccagacta ctgactcgag 1560
1561 gatccggctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg 1620
1621 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg 1680
1681 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc 1740
1741 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca 1800
1801 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt 1860
1861 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc ttgggctgca 1920
1921 ggtcgaggcg gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat 1980
1981 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca 2040
2041 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg 2100
2101 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg 2160
2161 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact 2220
2221 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct 2280
2281 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg 2340
2341 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt 2400
2401 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc 2460
2461 gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc 2520
2521 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga 2580
2581 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc 2640
2641 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt 2700
2701 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga 2760
2761 gcgggactct ggggttcgat aaaataaaag attttattta gtctccagaa aaagggggga 2820
2821 atgaaagacc ccacctgtag gtttggcaag ctagcttaag taacgccatt ttgcaaggca 2880
2881 tggaaaaata cataactgag aatagagaag ttcagatcaa ggtcaggaac agatggaaca 2940
2941 gctgaatatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca 3000
3001 agaacagatg gaacagctga atatgggcca aacaggatat ctgtggtaag cagttcctgc 3060
3061 cccggctcag ggccaagaac agatggtccc cagatgcggt ccagccctca gcagtttcta 3120
3121 gagaaccatc agatgtttcc agggtgcccc aaggacctga aatgaccctg tgccttattt 3180
3181 gaactaacca atcagttcgc ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcaa 3240
3241 taaaagagcc cacaacccct cactcggggc gccagtcctc cgattgactg agtcgcccgg 3300
3301 gtacccgtgt atccaataaa ccctcttgca gttgcatccg acttgtggtc tcgctgttcc 3360
3361 ttgggagggt ctcctctgag tgattgacta cccgtcagcg ggggtctttc atttgggggc 3420
3421 tcgtccggga tcgggagacc cctgcccagg gaccaccgac ccaccaccgg gaggtaagct 3480
3481 ggctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag 3540
3541 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca 3600
3601 gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg 3660
3661 tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt 3720
3721 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct 3780
3781 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa 3840
3841 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa 3900
3901 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc 3960
3961 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga 4020
4021 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc 4080
4081 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt 4140
4141 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct 4200
4201 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg 4260
4261 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta 4320
4321 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct 4380
4381 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa 4440
4441 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt 4500
4501 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta 4560
4561 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat 4620
4621 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa 4680
4681 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct 4740
4741 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta 4800
4801 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct 4860
4861 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg 4920
4921 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa 4980
4981 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt 5040
5041 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta 5100
5101 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca 5160
5161 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta 5220
5221 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct 5280
5281 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaacacgg gataataccg 5340
5341 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac 5400
5401 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact 5460
5461 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa 5520
5521 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt 5580
5581 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat 5640
5641 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg 5700
5701 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc 5760
5761 cctttcgtct tcaagaattg ctagcaattg ctagcaattg ctagcaattc ataccagatc 5820
5821 accgaaaact gtcctccaaa tgtgtccccc tcacactccc aaattcgcgg gcttctgcct 5880
5881 cttagaccac tctaccctat tccccacact caccggagcc aaagccgcgg cccttccgtt 5940
5941 tctttgct 5948
                            LOCUS       dna                      5948 bp                                   
FEATURES Location/Qualifiers
Other Gene 659..1468
/gene="psi_plus_pack other"
misc_binding 1470..1475
/dbxref="REBASE:EcoRI"
misc_binding 1476..1481
/dbxref="REBASE:ApaI"
misc_binding 1482..1487
/dbxref="REBASE:BglII"
misc_binding 1488..1493
/dbxref="REBASE:ClaI"
misc_binding 1494..1499
/dbxref="REBASE:HpaI"
misc_binding 1500..1507
/dbxref="REBASE:NotI"
tag 1527..1552
/gene="HA"
misc_binding 1555..1560
/dbxref="REBASE:XhoI"
misc_binding 1560..1565
/dbxref="REBASE:BamHI"
Regulatory_Seq 1567..1803
/gene="SV40ER reg"
Promoter 1590..1858
/gene="SV40 prom"
Promoter 1710..1912
/gene="SV40 prom"
Rep_Origin 1757..1834
/gene="SV40 origin"
misc_binding 1837..1849
/dbxref="REBASE:SfiI"
misc_binding 1888..1893
/dbxref="REBASE:StuI"
misc_binding 1907..1912
/dbxref="REBASE:HindIII"
misc_binding 1935..1940
/dbxref="REBASE:BclI"
ORF 1966..2760
/sequence="ORF_1 rf(1)"
Marker 1969..2757
/gene="NTP_II marker"
Other Gene 2862..3413
/gene="5_LTR2 other"
misc_binding 3117..3122
/dbxref="REBASE:XbaI"
misc_binding 3711..3716
/dbxref="REBASE:NdeI"
Rep_Origin 3935..4554
/gene="pBR322 origin"
Marker 4709..5569
/gene="amp marker"
ORF 4709..5569
/sequence="ORF_2 rf(5)"
Promoter 5611..5639
/gene="amp prom"
BASE COUNT 1370 a 1634 c 1531 g 1413 t 0 others
ORIGIN
1 tttgaaagac cccacccgta ggtggcaagc tagcttaagt aacgccactt tgcaaggcat
61 ggaaaaatac ataactgaga atagaaaagt tcagatcaag gtcaggaaca aagaaacagc
121 tgaataccaa acaggatatc tgtggtaagc ggttcctgcc ccggctcagg gccaagaaca
181 gatgagacag ctgagtgatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg
241 ctcggggcca agaacagatg gtccccagat gcggtccagc cctcagcagt ttctagtgaa
301 tcatcagatg tttccagggt gccccaagga cctgaaaatg accctgtacc ttatttgaac
361 taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc cgctctccga gctcaataaa
421 agagcccaca acccctcact cggcgcgcca gtcttccgat agactgcgtc gcccgggtac
481 ccgtattccc aataaagcct cttgctgttt gcatccgaat cgtggtctcg ctgttccttg
541 ggagggtctc ctctgagtga ttgactaccc acgacggggg tctttcattt gggggctcgt
601 ccgggatttg gagacccctg cccagggacc accgacccac caccgggagg taagctggcc
661 agcaacttat ctgtgtctgt ccgattgtct agtgtctatg tttgatgtta tgcgcctgcg
721 tctgtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa ctgacgagtt
781 ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc gtttttgtgg
841 cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg tggttctggt
901 aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt cggtttggaa
961 ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct
1021 gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc ccttaagttt
1081 gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa
1141 gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg tcggatggcc
1201 gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg tcttttcacc
1261 tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag ccttggcttt
1321 tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc ctcttcctcc
1381 atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta
1441 tccagccctc actccttctc taggcgccgg aattcgggcc cagatctatc gatgttaacg
1501 cggccgcgtc gactacgtaa tttaaattac ccatacgacg tcccagacta ctgactcgag
1561 gatccggctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
1621 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
1681 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
1741 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
1801 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
1861 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc ttgggctgca
1921 ggtcgaggcg gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat
1981 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
2041 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
2101 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg
2161 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
2221 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
2281 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
2341 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
2401 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
2461 gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc
2521 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
2581 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
2641 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
2701 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
2761 gcgggactct ggggttcgat aaaataaaag attttattta gtctccagaa aaagggggga
2821 atgaaagacc ccacctgtag gtttggcaag ctagcttaag taacgccatt ttgcaaggca
2881 tggaaaaata cataactgag aatagagaag ttcagatcaa ggtcaggaac agatggaaca
2941 gctgaatatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca
3001 agaacagatg gaacagctga atatgggcca aacaggatat ctgtggtaag cagttcctgc
3061 cccggctcag ggccaagaac agatggtccc cagatgcggt ccagccctca gcagtttcta
3121 gagaaccatc agatgtttcc agggtgcccc aaggacctga aatgaccctg tgccttattt
3181 gaactaacca atcagttcgc ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcaa
3241 taaaagagcc cacaacccct cactcggggc gccagtcctc cgattgactg agtcgcccgg
3301 gtacccgtgt atccaataaa ccctcttgca gttgcatccg acttgtggtc tcgctgttcc
3361 ttgggagggt ctcctctgag tgattgacta cccgtcagcg ggggtctttc atttgggggc
3421 tcgtccggga tcgggagacc cctgcccagg gaccaccgac ccaccaccgg gaggtaagct
3481 ggctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag
3541 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
3601 gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg
3661 tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
3721 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct
3781 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
3841 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
3901 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
3961 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
4021 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
4081 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
4141 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
4201 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
4261 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
4321 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
4381 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
4441 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
4501 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
4561 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
4621 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
4681 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
4741 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
4801 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
4861 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
4921 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
4981 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt
5041 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
5101 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
5161 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
5221 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
5281 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaacacgg gataataccg
5341 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
5401 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
5461 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
5521 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
5581 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
5641 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
5701 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
5761 cctttcgtct tcaagaattg ctagcaattg ctagcaattg ctagcaattc ataccagatc
5821 accgaaaact gtcctccaaa tgtgtccccc tcacactccc aaattcgcgg gcttctgcct
5881 cttagaccac tctaccctat tccccacact caccggagcc aaagccgcgg cccttccgtt
5941 tctttgct
//
***Note: The text map shown below is representative of the parental vector only and does not include the gene of interest. The sequence of the gene can be found through the accession number link on the product page.
1     tttgaaagaccccacccgtaggtggcaagctagcttaagtaacgccactttgcaaggcat 60
AAACTTTCTGGGGTGGGCATCCACCGTTCGATCGAATTCATTGCGGTGAAACGTTCCGTA

61 ggaaaaatacataactgagaatagaaaagttcagatcaaggtcaggaacaaagaaacagc 120
CCTTTTTATGTATTGACTCTTATCTTTTCAAGTCTAGTTCCAGTCCTTGTTTCTTTGTCG

121 tgaataccaaacaggatatctgtggtaagcggttcctgccccggctcagggccaagaaca 180
ACTTATGGTTTGTCCTATAGACACCATTCGCCAAGGACGGGGCCGAGTCCCGGTTCTTGT

181 gatgagacagctgagtgatgggccaaacaggatatctgtggtaagcagttcctgccccgg 240
CTACTCTGTCGACTCACTACCCGGTTTGTCCTATAGACACCATTCGTCAAGGACGGGGCC

241 ctcggggccaagaacagatggtccccagatgcggtccagccctcagcagtttctagtgaa 300
GAGCCCCGGTTCTTGTCTACCAGGGGTCTACGCCAGGTCGGGAGTCGTCAAAGATCACTT

301 tcatcagatgtttccagggtgccccaaggacctgaaaatgaccctgtaccttatttgaac 360
AGTAGTCTACAAAGGTCCCACGGGGTTCCTGGACTTTTACTGGGACATGGAATAAACTTG

361 taaccaatcagttcgcttctcgcttctgttcgcgcgcttccgctctccgagctcaataaa 420
ATTGGTTAGTCAAGCGAAGAGCGAAGACAAGCGCGCGAAGGCGAGAGGCTCGAGTTATTT

421 agagcccacaacccctcactcggcgcgccagtcttccgatagactgcgtcgcccgggtac 480
TCTCGGGTGTTGGGGAGTGAGCCGCGCGGTCAGAAGGCTATCTGACGCAGCGGGCCCATG

481 ccgtattcccaataaagcctcttgctgtttgcatccgaatcgtggtctcgctgttccttg 540
GGCATAAGGGTTATTTCGGAGAACGACAAACGTAGGCTTAGCACCAGAGCGACAAGGAAC

541 ggagggtctcctctgagtgattgactacccacgacgggggtctttcatttgggggctcgt 600
CCTCCCAGAGGAGACTCACTAACTGATGGGTGCTGCCCCCAGAAAGTAAACCCCCGAGCA

psi_plus_pack other(659,1468)>>>
|
601 ccgggatttggagacccctgcccagggaccaccgacccaccaccgggaggtaagctggcc 660
GGCCCTAAACCTCTGGGGACGGGTCCCTGGTGGCTGGGTGGTGGCCCTCCATTCGACCGG

661 agcaacttatctgtgtctgtccgattgtctagtgtctatgtttgatgttatgcgcctgcg 720
TCGTTGAATAGACACAGACAGGCTAACAGATCACAGATACAAACTACAATACGCGGACGC

721 tctgtactagttagctaactagctctgtatctggcggacccgtggtggaactgacgagtt 780
AGACATGATCAATCGATTGATCGAGACATAGACCGCCTGGGCACCACCTTGACTGCTCAA

781 ctgaacacccggccgcaaccctgggagacgtcccagggactttgggggccgtttttgtgg 840
GACTTGTGGGCCGGCGTTGGGACCCTCTGCAGGGTCCCTGAAACCCCCGGCAAAAACACC

841 cccgacctgaggaagggagtcgatgtggaatccgaccccgtcaggatatgtggttctggt 900
GGGCTGGACTCCTTCCCTCAGCTACACCTTAGGCTGGGGCAGTCCTATACACCAAGACCA

901 aggagacgagaacctaaaacagttcccgcctccgtctgaatttttgctttcggtttggaa 960
TCCTCTGCTCTTGGATTTTGTCAAGGGCGGAGGCAGACTTAAAAACGAAAGCCAAACCTT

961 ccgaagccgcgcgtcttgtctgctgcagcgctgcagcatcgttctgtgttgtctctgtct 1020
GGCTTCGGCGCGCAGAACAGACGACGTCGCGACGTCGTAGCAAGACACAACAGAGACAGA

1021 gactgtgtttctgtatttgtctgaaaattagggccagactgttaccactcccttaagttt 1080
CTGACACAAAGACATAAACAGACTTTTAATCCCGGTCTGACAATGGTGAGGGAATTCAAA

1081 gaccttaggtcactggaaagatgtcgagcggatcgctcacaaccagtcggtagatgtcaa 1140
CTGGAATCCAGTGACCTTTCTACAGCTCGCCTAGCGAGTGTTGGTCAGCCATCTACAGTT

1141 gaagagacgttgggttaccttctgctctgcagaatggccaacctttaacgtcggatggcc 1200
CTTCTCTGCAACCCAATGGAAGACGAGACGTCTTACCGGTTGGAAATTGCAGCCTACCGG

1201 gcgagacggcacctttaaccgagacctcatcacccaggttaagatcaaggtcttttcacc 1260
CGCTCTGCCGTGGAAATTGGCTCTGGAGTAGTGGGTCCAATTCTAGTTCCAGAAAAGTGG

1261 tggcccgcatggacacccagaccaggtcccctacatcgtgacctgggaagccttggcttt 1320
ACCGGGCGTACCTGTGGGTCTGGTCCAGGGGATGTAGCACTGGACCCTTCGGAACCGAAA

1321 tgacccccctccctgggtcaagccctttgtacaccctaagcctccgcctcctcttcctcc 1380
ACTGGGGGGAGGGACCCAGTTCGGGAAACATGTGGGATTCGGAGGCGGAGGAGAAGGAGG

1381 atccgccccgtctctcccccttgaacctcctcgttcgaccccgcctcgatcctcccttta 1440
TAGGCGGGGCAGAGAGGGGGAACTTGGAGGAGCAAGCTGGGGCGGAGCTAGGAGGGAAAT

BglII
|
EcoRI ApaI ClaI HpaI
| | | | |
1441 tccagccctcactccttctctaggcgccggaattcgggcccagatctatcgatgttaacg 1500
AGGTCGGGAGTGAGGAAGAGATCCGCGGCCTTAAGCCCGGGTCTAGATAGCTACAATTGC

NotI HA(1527,1552)>>> XhoI
| | |
1501 cggccgcgtcgactacgtaatttaaattacccatacgacgtcccagactactgactcgag 1560
GCCGGCGCAGCTGATGCATTAAATTTAATGGGTATGCTGCAGGGTCTGATGACTGAGCTC

SV40 prom(1590,1858)>>>
|
BamHI SV40ER reg(1567,1803)<<<
| | |
1561 gatccggctgtggaatgtgtgtcagttagggtgtggaaagtccccaggctccccagcagg 1620
CTAGGCCGACACCTTACACACAGTCAATCCCACACCTTTCAGGGGTCCGAGGGGTCGTCC

1621 cagaagtatgcaaagcatgcatctcaattagtcagcaaccaggtgtggaaagtccccagg 1680
GTCTTCATACGTTTCGTACGTAGAGTTAATCAGTCGTTGGTCCACACCTTTCAGGGGTCC

SV40 prom(1710,1912)>>>
|
1681 ctccccagcaggcagaagtatgcaaagcatgcatctcaattagtcagcaaccatagtccc 1740
GAGGGGTCGTCCGTCTTCATACGTTTCGTACGTAGAGTTAATCAGTCGTTGGTATCAGGG

SV40 origin(1757,1834)>>>
|
1741 gcccctaactccgcccatcccgcccctaactccgcccagttccgcccattctccgcccca 1800
CGGGGATTGAGGCGGGTAGGGCGGGGATTGAGGCGGGTCAAGGCGGGTAAGAGGCGGGGT

SfiI
|
1801 tggctgactaattttttttatttatgcagaggccgaggccgcctcggcctctgagctatt 1860
ACCGACTGATTAAAAAAAATAAATACGTCTCCGGCTCCGGCGGAGCCGGAGACTCGATAA

StuI HindIII
| |
1861 ccagaagtagtgaggaggcttttttggaggcctaggcttttgcaaaaagcttgggctgca 1920
GGTCTTCATCACTCCTCCGAAAAAACCTCCGGATCCGAAAACGTTTTTCGAACCCGACGT

NTP_II marker(1969,2757)>>>
|
BclI ORF_1 rf(1)(1966,2760)>>>
| | |
1921 ggtcgaggcggatctgatcaagagacaggatgaggatcgtttcgcatgattgaacaagat 1980
CCAGCTCCGCCTAGACTAGTTCTCTGTCCTACTCCTAGCAAAGCGTACTAACTTGTTCTA

1981 ggattgcacgcaggttctccggccgcttgggtggagaggctattcggctatgactgggca 2040
CCTAACGTGCGTCCAAGAGGCCGGCGAACCCACCTCTCCGATAAGCCGATACTGACCCGT

2041 caacagacaatcggctgctctgatgccgccgtgttccggctgtcagcgcaggggcgcccg 2100
GTTGTCTGTTAGCCGACGAGACTACGGCGGCACAAGGCCGACAGTCGCGTCCCCGCGGGC

2101 gttctttttgtcaagaccgacctgtccggtgccctgaatgaactgcaggacgaggcagcg 2160
CAAGAAAAACAGTTCTGGCTGGACAGGCCACGGGACTTACTTGACGTCCTGCTCCGTCGC

2161 cggctatcgtggctggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcact 2220
GCCGATAGCACCGACCGGTGCTGCCCGCAAGGAACGCGTCGACACGAGCTGCAACAGTGA

2221 gaagcgggaagggactggctgctattgggcgaagtgccggggcaggatctcctgtcatct 2280
CTTCGCCCTTCCCTGACCGACGATAACCCGCTTCACGGCCCCGTCCTAGAGGACAGTAGA

2281 caccttgctcctgccgagaaagtatccatcatggctgatgcaatgcggcggctgcatacg 2340
GTGGAACGAGGACGGCTCTTTCATAGGTAGTACCGACTACGTTACGCCGCCGACGTATGC

2341 cttgatccggctacctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgt 2400
GAACTAGGCCGATGGACGGGTAAGCTGGTGGTTCGCTTTGTAGCGTAGCTCGCTCGTGCA

2401 actcggatggaagccggtcttgtcgatcaggatgatctggacgaagagcatcaggggctc 2460
TGAGCCTACCTTCGGCCAGAACAGCTAGTCCTACTAGACCTGCTTCTCGTAGTCCCCGAG

2461 gcgccagccgaactgttcgccaggctcaaggcgcgcatgcccgacggcgaggatctcgtc 2520
CGCGGTCGGCTTGACAAGCGGTCCGAGTTCCGCGCGTACGGGCTGCCGCTCCTAGAGCAG

2521 gtgacccatggcgatgcctgcttgccgaatatcatggtggaaaatggccgcttttctgga 2580
CACTGGGTACCGCTACGGACGAACGGCTTATAGTACCACCTTTTACCGGCGAAAAGACCT

2581 ttcatcgactgtggccggctgggtgtggcggaccgctatcaggacatagcgttggctacc 2640
AAGTAGCTGACACCGGCCGACCCACACCGCCTGGCGATAGTCCTGTATCGCAACCGATGG

2641 cgtgatattgctgaagagcttggcggcgaatgggctgaccgcttcctcgtgctttacggt 2700
GCACTATAACGACTTCTCGAACCGCCGCTTACCCGACTGGCGAAGGAGCACGAAATGCCA

2701 atcgccgctcccgattcgcagcgcatcgccttctatcgccttcttgacgagttcttctga 2760
TAGCGGCGAGGGCTAAGCGTCGCGTAGCGGAAGATAGCGGAAGAACTGCTCAAGAAGACT

2761 gcgggactctggggttcgataaaataaaagattttatttagtctccagaaaaagggggga 2820
CGCCCTGAGACCCCAAGCTATTTTATTTTCTAAAATAAATCAGAGGTCTTTTTCCCCCCT

5_LTR2 other(2862,3413)>>>
|
2821 atgaaagaccccacctgtaggtttggcaagctagcttaagtaacgccattttgcaaggca 2880
TACTTTCTGGGGTGGACATCCAAACCGTTCGATCGAATTCATTGCGGTAAAACGTTCCGT

2881 tggaaaaatacataactgagaatagagaagttcagatcaaggtcaggaacagatggaaca 2940
ACCTTTTTATGTATTGACTCTTATCTCTTCAAGTCTAGTTCCAGTCCTTGTCTACCTTGT

2941 gctgaatatgggccaaacaggatatctgtggtaagcagttcctgccccggctcagggcca 3000
CGACTTATACCCGGTTTGTCCTATAGACACCATTCGTCAAGGACGGGGCCGAGTCCCGGT

3001 agaacagatggaacagctgaatatgggccaaacaggatatctgtggtaagcagttcctgc 3060
TCTTGTCTACCTTGTCGACTTATACCCGGTTTGTCCTATAGACACCATTCGTCAAGGACG

XbaI
|
3061 cccggctcagggccaagaacagatggtccccagatgcggtccagccctcagcagtttcta 3120
GGGCCGAGTCCCGGTTCTTGTCTACCAGGGGTCTACGCCAGGTCGGGAGTCGTCAAAGAT

3121 gagaaccatcagatgtttccagggtgccccaaggacctgaaatgaccctgtgccttattt 3180
CTCTTGGTAGTCTACAAAGGTCCCACGGGGTTCCTGGACTTTACTGGGACACGGAATAAA

3181 gaactaaccaatcagttcgcttctcgcttctgttcgcgcgcttctgctccccgagctcaa 3240
CTTGATTGGTTAGTCAAGCGAAGAGCGAAGACAAGCGCGCGAAGACGAGGGGCTCGAGTT

3241 taaaagagcccacaacccctcactcggggcgccagtcctccgattgactgagtcgcccgg 3300
ATTTTCTCGGGTGTTGGGGAGTGAGCCCCGCGGTCAGGAGGCTAACTGACTCAGCGGGCC

3301 gtacccgtgtatccaataaaccctcttgcagttgcatccgacttgtggtctcgctgttcc 3360
CATGGGCACATAGGTTATTTGGGAGAACGTCAACGTAGGCTGAACACCAGAGCGACAAGG

3361 ttgggagggtctcctctgagtgattgactacccgtcagcgggggtctttcatttgggggc 3420
AACCCTCCCAGAGGAGACTCACTAACTGATGGGCAGTCGCCCCCAGAAAGTAAACCCCCG

3421 tcgtccgggatcgggagacccctgcccagggaccaccgacccaccaccgggaggtaagct 3480
AGCAGGCCCTAGCCCTCTGGGGACGGGTCCCTGGTGGCTGGGTGGTGGCCCTCCATTCGA

3481 ggctgcctcgcgcgtttcggtgatgacggtgaaaacctctgacacatgcagctcccggag 3540
CCGACGGAGCGCGCAAAGCCACTACTGCCACTTTTGGAGACTGTGTACGTCGAGGGCCTC

3541 acggtcacagcttgtctgtaagcggatgccgggagcagacaagcccgtcagggcgcgtca 3600
TGCCAGTGTCGAACAGACATTCGCCTACGGCCCTCGTCTGTTCGGGCAGTCCCGCGCAGT

3601 gcgggtgttggcgggtgtcggggcgcagccatgacccagtcacgtagcgatagcggagtg 3660
CGCCCACAACCGCCCACAGCCCCGCGTCGGTACTGGGTCAGTGCATCGCTATCGCCTCAC

NdeI
|
3661 tatactggcttaactatgcggcatcagagcagattgtactgagagtgcaccatatgcggt 3720
ATATGACCGAATTGATACGCCGTAGTCTCGTCTAACATGACTCTCACGTGGTATACGCCA

3721 gtgaaataccgcacagatgcgtaaggagaaaataccgcatcaggcgctcttccgcttcct 3780
CACTTTATGGCGTGTCTACGCATTCCTCTTTTATGGCGTAGTCCGCGAGAAGGCGAAGGA

3781 cgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaa 3840
GCGAGTGACTGAGCGACGCGAGCCAGCAAGCCGACGCCGCTCGCCATAGTCGAGTGAGTT

3841 aggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaa 3900
TCCGCCATTATGCCAATAGGTGTCTTAGTCCCCTATTGCGTCCTTTCTTGTACACTCGTT

pBR322 origin(3935,4554)<<<
|
3901 aaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggc 3960
TTCCGGTCGTTTTCCGGTCCTTGGCATTTTTCCGGCGCAACGACCGCAAAAAGGTATCCG

3961 tccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccga 4020
AGGCGGGGGGACTGCTCGTAGTGTTTTTAGCTGCGAGTTCAGTCTCCACCGCTTTGGGCT

4021 caggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttc 4080
GTCCTGATATTTCTATGGTCCGCAAAGGGGGACCTTCGAGGGAGCACGCGAGAGGACAAG

4081 cgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgcttt 4140
GCTGGGACGGCGAATGGCCTATGGACAGGCGGAAAGAGGGAAGCCCTTCGCACCGCGAAA

4141 ctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggct 4200
GAGTATCGAGTGCGACATCCATAGAGTCAAGCCACATCCAGCAAGCGAGGTTCGACCCGA

4201 gtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttg 4260
CACACGTGCTTGGGGGGCAAGTCGGGCTGGCGACGCGGAATAGGCCATTGATAGCAGAAC

4261 agtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggatta 4320
TCAGGTTGGGCCATTCTGTGCTGAATAGCGGTGACCGTCGTCGGTGACCATTGTCCTAAT

4321 gcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggct 4380
CGTCTCGCTCCATACATCCGCCACGATGTCTCAAGAACTTCACCACCGGATTGATGCCGA

4381 acactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaa 4440
TGTGATCTTCCTGTCATAAACCATAGACGCGAGACGACTTCGGTCAATGGAAGCCTTTTT

4441 gagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgttt 4500
CTCAACCATCGAGAACTAGGCCGTTTGTTTGGTGGCGACCATCGCCACCAAAAAAACAAA

4501 gcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttcta 4560
CGTTCGTCGTCTAATGCGCGTCTTTTTTTCCTAGAGTTCTTCTAGGAAACTAGAAAAGAT

4561 cggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattat 4620
GCCCCAGACTGCGAGTCACCTTGCTTTTGAGTGCAATTCCCTAAAACCAGTACTCTAATA

4621 caaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaa 4680
GTTTTTCCTAGAAGTGGATCTAGGAAAATTTAATTTTTACTTCAAAATTTAGTTAGATTT

ORF_2 rf(5)(4709,5569)<<<
|
amp marker(4709,5569)<<<
|
4681 gtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatct 4740
CATATATACTCATTTGAACCAGACTGTCAATGGTTACGAATTAGTCACTCCGTGGATAGA

4741 cagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataacta 4800
GTCGCTAGACAGATAAAGCAAGTAGGTATCAACGGACTGAGGGGCAGCACATCTATTGAT

4801 cgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgct 4860
GCTATGCCCTCCCGAATGGTAGACCGGGGTCACGACGTTACTATGGCGCTCTGGGTGCGA

4861 caccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtg 4920
GTGGCCGAGGTCTAAATAGTCGTTATTTGGTCGGTCGGCCTTCCCGGCTCGCGTCTTCAC

4921 gtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaa 4980
CAGGACGTTGAAATAGGCGGAGGTAGGTCAGATAATTAACAACGGCCCTTCGATCTCATT

4981 gtagttcgccagttaatagtttgcgcaacgttgttgccattgctgcaggcatcgtggtgt 5040
CATCAAGCGGTCAATTATCAAACGCGTTGCAACAACGGTAACGACGTCCGTAGCACCACA

5041 cacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagtta 5100
GTGCGAGCAGCAAACCATACCGAAGTAAGTCGAGGCCAAGGGTTGCTAGTTCCGCTCAAT

5101 catgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtca 5160
GTACTAGGGGGTACAACACGTTTTTTCGCCAATCGAGGAAGCCAGGAGGCTAGCAACAGT

5161 gaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctctta 5220
CTTCATTCAACCGGCGTCACAATAGTGAGTACCAATACCGTCGTGACGTATTAAGAGAAT

5221 ctgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattct 5280
GACAGTACGGTAGGCATTCTACGAAAAGACACTGACCACTCATGAGTTGGTTCAGTAAGA

5281 gagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaacacgggataataccg 5340
CTCTTATCACATACGCCGCTGGCTCAACGAGAACGGGCCGCAGTTGTGCCCTATTATGGC

5341 cgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaac 5400
GCGGTGTATCGTCTTGAAATTTTCACGAGTAGTAACCTTTTGCAAGAAGCCCCGCTTTTG

5401 tctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaact 5460
AGAGTTCCTAGAATGGCGACAACTCTAGGTCAAGCTACATTGGGTGAGCACGTGGGTTGA

5461 gatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaa 5520
CTAGAAGTCGTAGAAAATGAAAGTGGTCGCAAAGACCCACTCGTTTTTGTCCTTCCGTTT

5521 atgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttccttt 5580
TACGGCGTTTTTTCCCTTATTCCCGCTGTGCCTTTACAACTTATGAGTATGAGAAGGAAA

amp prom(5611,5639)<<<
|
5581 ttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaat 5640
AAGTTATAATAACTTCGTAAATAGTCCCAATAACAGAGTACTCGCCTATGTATAAACTTA

5641 gtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctg 5700
CATAAATCTTTTTATTTGTTTATCCCCAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGAC

5701 acgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggc 5760
TGCAGATTCTTTGGTAATAATAGTACTGTAATTGGATATTTTTATCCGCATAGTGCTCCG

5761 cctttcgtcttcaagaattgctagcaattgctagcaattgctagcaattcataccagatc 5820
GGAAAGCAGAAGTTCTTAACGATCGTTAACGATCGTTAACGATCGTTAAGTATGGTCTAG

5821 accgaaaactgtcctccaaatgtgtccccctcacactcccaaattcgcgggcttctgcct 5880
TGGCTTTTGACAGGAGGTTTACACAGGGGGAGTGTGAGGGTTTAAGCGCCCGAAGACGGA

5881 cttagaccactctaccctattccccacactcaccggagccaaagccgcggcccttccgtt 5940
GAATCTGGTGAGATGGGATAAGGGGTGTGAGTGGCCTCGGTTTCGGCGCCGGGAAGGCAA

5941 tctttgct 5948
AGAAACGA

                            	Misc. Feature (6 total)
5'-LTR
Start: 1 End: 600
Original Location Description:
1..600
MCS
Start: 1437 End: 1497
Original Location Description:
1437..1497
SVpro
Start: 1574 End: 1920
Original Location Description:
1547..1893
Neo-R
Start: 1966 End: 2760
Original Location Description:
1939..2733
3'-LTR
Start: 2820 End: 3413
Original Location Description:
2793..3386
Amp
Start: 4711 End: 5569 (Complementary)
Original Location Description:
complement(4684..5542)