pPromoter-ERT2-CRE-ERT2
| Vector Name | pPromoter-ERT2-CRE-ERT2 |
| VectorType | lentivector |
| Antibiotic Information | Kanamycin |
| Sequencing Primers | bGH-Rev: TAG AAG GCA CAG TCG AGG |
| Additional Information | No Additional Information |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt 61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa 121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg 181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt 241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg 301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag 361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta 481 gcgaaagctc agatctggat ctcccgatcc cctatggtcg actctcagta caatctgctc 541 tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta 601 gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcttaagatt 661 taaataagct tggtaccgag ctcggatcca ctagtccagt gtggtggaat tcccgggtga 721 gccgccacca tggctggaga catgagagct gccaaccttt ggccaagccc gctcatgatc 781 aaacgctcta agaagaacag cctggccttg tccctgacgg ccgaccagat ggtcagtgcc 841 ttgttggatg ctgagccccc catactctat tccgagtatg atcctaccag acccttcagt 901 gaagcttcga tgatgggctt actgaccaac ctggcagaca gggagctggt tcacatgatc 961 aactgggcga agagggtgcc aggctttgtg gatttgaccc tccatgatca ggtccacctt 1021 ctagaatgtg cctggctaga gatcctgatg attggtctcg tctggcgctc catggagcac 1081 ccagtgaagc tactgtttgc tcctaacttg ctcttggaca ggaaccaggg aaaatgtgta 1141 gagggcatgg tggagatctt cgacatgctg ctggctacat catctcggtt ccgcatgatg 1201 aatctgcagg gagaggagtt tgtgtgcctc aaatctatta ttttgcttaa ttctggagtg 1261 tacacatttc tgtccagcac cctgaagtct ctggaagaga aggaccatat ccaccgagtc 1321 ctggacaaga tcacagacac tttgatccac ctgatggcca aggcaggcct gaccctgcag 1381 cagcagcacc agcggctggc ccagctcctc ctcatcctct cccacatcag gcacatgagt 1441 aacaaaggca tggagcatct gtacagcatg aagtgcaaga acgtggtgcc cctctatgac 1501 ctgctgctgg aggcggcgga cgcccaccgc ctacatgcgc ccactagccg tggaggggca 1561 tccgtggagg agacggacca aagccacttg gccactgcgg gctctacttc atcgcattcc 1621 ttgcaaaagt attacatcac gggggaggca gagggtttcc ctgccacagc tgtcgacaat 1681 ttactgaccg tacaccaaaa tttgcctgca ttaccggtcg atgcaacgag tgatgaggtt 1741 cgcaagaacc tgatggacat gttcagggat cgccaggcgt tttctgagca tacctggaaa 1801 atgcttctgt ccgtttgccg gtcgtgggcg gcatggtgca agttgaataa ccggaaatgg 1861 tttcccgcag aacctgaaga tgttcgcgat tatcttctat atcttcaggc gcgcggtctg 1921 gcagtaaaaa ctatccagca acatttgggc cagctaaaca tgcttcatcg tcggtccggg 1981 ctgccacgac caagtgacag caatgctgtt tcactggtta tgcggcggat ccgaaaagaa 2041 aacgttgatg ccggtgaacg tgcaaaacag gctctagcgt tcgaacgcac tgatttcgac 2101 caggttcgtt cactcatgga aaatagcgat cgctgccagg atatacgtaa tctggcattt 2161 ctggggattg cttataacac cctgttacgt atagccgaaa ttgccaggat cagggttaaa 2221 gatatctcac gtactgacgg tgggagaatg ttaatccata ttggcagaac gaaaacgctg 2281 gttagcaccg caggtgtaga gaaggcactt agcctggggg taactaaact ggtcgagcga 2341 tggatttccg tctctggtgt agctgatgat ccgaataact acctgttttg ccgggtcaga 2401 aaaaatggtg ttgccgcgcc atctgccacc agccagctat caactcgcgc cctggaaggg 2461 atttttgaag caactcatcg attgatttac ggcgctaagg atgactctgg tcagagatac 2521 ctggcctggt ctggacacag tgcccgtgtc ggagccgcgc gagatatggc ccgcgctgga 2581 gtttcaatac cggagatcat gcaagctggt ggctggacca atgtaaatat tgtcatgaac 2641 tatatccgta acctggatag tgaaacaggg gcaatggtgc gcctgctgga agatggcgat 2701 ctcgagccat ctgctggaga catgagagct gccaaccttt ggccaagccc gctcatgatc 2761 aaacgctcta agaagaacag cctggccttg tccctgacgg ccgaccagat ggtcagtgcc 2821 ttgttggatg ctgagccccc catactctat tccgagtatg atcctaccag acccttcagt 2881 gaagcttcga tgatgggctt actgaccaac ctggcagaca gggagctggt tcacatgatc 2941 aactgggcga agagggtgcc aggctttgtg gatttgaccc tccatgatca ggtccacctt 3001 ctagaatgtg cctggctaga gatcctgatg attggtctcg tctggcgctc catggagcac 3061 ccagtgaagc tactgtttgc tcctaacttg ctcttggaca ggaaccaggg aaaatgtgta 3121 gagggcatgg tggagatctt cgacatgctg ctggctacat catctcggtt ccgcatgatg 3181 aatctgcagg gagaggagtt tgtgtgcctc aaatctatta ttttgcttaa ttctggagtg 3241 tacacatttc tgtccagcac cctgaagtct ctggaagaga aggaccatat ccaccgagtc 3301 ctggacaaga tcacagacac tttgatccac ctgatggcca aggcaggcct gaccctgcag 3361 cagcagcacc agcggctggc ccagctcctc ctcatcctct cccacatcag gcacatgagt 3421 aacaaaggca tggagcatct gtacagcatg aagtgcaaga acgtggtgcc cctctatgac 3481 ctgctgctgg aggcggcgga cgcccaccgc ctacatgcgc ccactagccg tggaggggca 3541 tccgtggagg agacggacca aagccacttg gccactgcgg gctctacttc atcgcattcc 3601 ttgcaaaagt attacatcac gggggaggca gagggtttcc ctgccacagc ttgatagcgg 3661 ccgctcgagt ctagagggcc cgtttaaacc gctgatcagc ctcgactgtg ccttctagtt 3721 gccagccatc tgttgttgcc cgggcgcgat cgctgcccct cccccgtgcc ttccttgacc 3781 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt 3841 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat 3901 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa 3961 agaaccagca gatcgatctg catctatgtc gggtgcggag aaagaggtaa tgaaatggca 4021 ttatgggtat tatgggtctg cattaatgaa tcggccaacg gatcccggtg tgaaataccg 4081 cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac 4141 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata 4201 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa 4261 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct 4321 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 4381 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 4441 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca 4501 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 4561 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 4621 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 4681 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg 4741 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 4801 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag 4861 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 4921 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc 4981 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag 5041 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt 5101 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag 5161 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca 5221 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact 5281 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca 5341 gttaatagtt tgcgcaacgt tgttgaaaaa ggatcttcac ctagatcctt ttcacgtaga 5401 aagccagtcc gcagaaacgg tgctgacccc ggatgaatgt cagctactgg gctatctgga 5461 caagggaaaa cgcaagcgca aagagaaagc aggtagcttg cagtgggctt acatggcgat 5521 agctagactg ggcggtttta tggacagcaa gcgaaccgga attgccagct ggggcgccct 5581 ctggtaaggt tgggaagccc tgcaaagtaa actggatggc tttctcgccg ccaaggatct 5641 gatggcgcag gggatcaagc tctgatcaag agacaggatg aggatcgttt cgcatgattg 5701 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg 5761 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg 5821 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg 5881 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg 5941 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc 6001 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc 6061 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc 6121 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc 6181 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg 6241 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct 6301 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt 6361 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc 6421 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt 6481 tcttctgaat tttgttaaaa tttttgttaa atcagctcat tttttaacca ataggccgaa 6541 atcggcaaca tcccttataa atcaaaagaa tagaccgcga tagggttgag tgttgttcca 6601 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc 6661 gtctatcagg gcgatggccc actacgtgaa ccatcaccca aatcaagttt tttgcggtcg 6721 aggtgccgta aagctctaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg 6781 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg 6841 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgcgcg cttaatgcgc 6901 cgctacaggg cgcgtccatt cgccattcag gatcgaatta attcttaatt aacatcatca 6961 ataatatacc tt
LOCUS dna 6972 bp FEATURES Location/Qualifiers Other Gene 162..310 /gene="Encap other" Promoter 365..393 /gene="amp prom" misc_binding 457..462 /dbxref="REBASE:HpaI" misc_binding 672..677 /dbxref="REBASE:KpnI" misc_binding 678..683 /dbxref="REBASE:SacI" misc_binding 707..712 /dbxref="REBASE:EcoRI" ORF 730..3654 /sequence="ORF_1 rf(1)" misc_binding 1884..1889 /dbxref="REBASE:NruI" misc_binding 2221..2226 /dbxref="REBASE:EcoRV" misc_binding 3657..3664 /dbxref="REBASE:NotI" misc_binding 3676..3681 /dbxref="REBASE:ApaI" Rep_Origin 4284..4903 /gene="pBR322 origin" Promoter 5556..5605 /gene="NEOKAN prom" ORF 5694..6488 /sequence="ORF_2 rf(3)" Marker 5697..6485 /gene="NTP_II marker" Rep_Origin 6593..6898 /gene="f1 origin" BASE COUNT 1673 a 1754 c 1927 g 1618 t 0 others ORIGIN 1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt 61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa 121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg 181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt 241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg 301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag 361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta 481 gcgaaagctc agatctggat ctcccgatcc cctatggtcg actctcagta caatctgctc 541 tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta 601 gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcttaagatt 661 taaataagct tggtaccgag ctcggatcca ctagtccagt gtggtggaat tcccgggtga 721 gccgccacca tggctggaga catgagagct gccaaccttt ggccaagccc gctcatgatc 781 aaacgctcta agaagaacag cctggccttg tccctgacgg ccgaccagat ggtcagtgcc 841 ttgttggatg ctgagccccc catactctat tccgagtatg atcctaccag acccttcagt 901 gaagcttcga tgatgggctt actgaccaac ctggcagaca gggagctggt tcacatgatc 961 aactgggcga agagggtgcc aggctttgtg gatttgaccc tccatgatca ggtccacctt 1021 ctagaatgtg cctggctaga gatcctgatg attggtctcg tctggcgctc catggagcac 1081 ccagtgaagc tactgtttgc tcctaacttg ctcttggaca ggaaccaggg aaaatgtgta 1141 gagggcatgg tggagatctt cgacatgctg ctggctacat catctcggtt ccgcatgatg 1201 aatctgcagg gagaggagtt tgtgtgcctc aaatctatta ttttgcttaa ttctggagtg 1261 tacacatttc tgtccagcac cctgaagtct ctggaagaga aggaccatat ccaccgagtc 1321 ctggacaaga tcacagacac tttgatccac ctgatggcca aggcaggcct gaccctgcag 1381 cagcagcacc agcggctggc ccagctcctc ctcatcctct cccacatcag gcacatgagt 1441 aacaaaggca tggagcatct gtacagcatg aagtgcaaga acgtggtgcc cctctatgac 1501 ctgctgctgg aggcggcgga cgcccaccgc ctacatgcgc ccactagccg tggaggggca 1561 tccgtggagg agacggacca aagccacttg gccactgcgg gctctacttc atcgcattcc 1621 ttgcaaaagt attacatcac gggggaggca gagggtttcc ctgccacagc tgtcgacaat 1681 ttactgaccg tacaccaaaa tttgcctgca ttaccggtcg atgcaacgag tgatgaggtt 1741 cgcaagaacc tgatggacat gttcagggat cgccaggcgt tttctgagca tacctggaaa 1801 atgcttctgt ccgtttgccg gtcgtgggcg gcatggtgca agttgaataa ccggaaatgg 1861 tttcccgcag aacctgaaga tgttcgcgat tatcttctat atcttcaggc gcgcggtctg 1921 gcagtaaaaa ctatccagca acatttgggc cagctaaaca tgcttcatcg tcggtccggg 1981 ctgccacgac caagtgacag caatgctgtt tcactggtta tgcggcggat ccgaaaagaa 2041 aacgttgatg ccggtgaacg tgcaaaacag gctctagcgt tcgaacgcac tgatttcgac 2101 caggttcgtt cactcatgga aaatagcgat cgctgccagg atatacgtaa tctggcattt 2161 ctggggattg cttataacac cctgttacgt atagccgaaa ttgccaggat cagggttaaa 2221 gatatctcac gtactgacgg tgggagaatg ttaatccata ttggcagaac gaaaacgctg 2281 gttagcaccg caggtgtaga gaaggcactt agcctggggg taactaaact ggtcgagcga 2341 tggatttccg tctctggtgt agctgatgat ccgaataact acctgttttg ccgggtcaga 2401 aaaaatggtg ttgccgcgcc atctgccacc agccagctat caactcgcgc cctggaaggg 2461 atttttgaag caactcatcg attgatttac ggcgctaagg atgactctgg tcagagatac 2521 ctggcctggt ctggacacag tgcccgtgtc ggagccgcgc gagatatggc ccgcgctgga 2581 gtttcaatac cggagatcat gcaagctggt ggctggacca atgtaaatat tgtcatgaac 2641 tatatccgta acctggatag tgaaacaggg gcaatggtgc gcctgctgga agatggcgat 2701 ctcgagccat ctgctggaga catgagagct gccaaccttt ggccaagccc gctcatgatc 2761 aaacgctcta agaagaacag cctggccttg tccctgacgg ccgaccagat ggtcagtgcc 2821 ttgttggatg ctgagccccc catactctat tccgagtatg atcctaccag acccttcagt 2881 gaagcttcga tgatgggctt actgaccaac ctggcagaca gggagctggt tcacatgatc 2941 aactgggcga agagggtgcc aggctttgtg gatttgaccc tccatgatca ggtccacctt 3001 ctagaatgtg cctggctaga gatcctgatg attggtctcg tctggcgctc catggagcac 3061 ccagtgaagc tactgtttgc tcctaacttg ctcttggaca ggaaccaggg aaaatgtgta 3121 gagggcatgg tggagatctt cgacatgctg ctggctacat catctcggtt ccgcatgatg 3181 aatctgcagg gagaggagtt tgtgtgcctc aaatctatta ttttgcttaa ttctggagtg 3241 tacacatttc tgtccagcac cctgaagtct ctggaagaga aggaccatat ccaccgagtc 3301 ctggacaaga tcacagacac tttgatccac ctgatggcca aggcaggcct gaccctgcag 3361 cagcagcacc agcggctggc ccagctcctc ctcatcctct cccacatcag gcacatgagt 3421 aacaaaggca tggagcatct gtacagcatg aagtgcaaga acgtggtgcc cctctatgac 3481 ctgctgctgg aggcggcgga cgcccaccgc ctacatgcgc ccactagccg tggaggggca 3541 tccgtggagg agacggacca aagccacttg gccactgcgg gctctacttc atcgcattcc 3601 ttgcaaaagt attacatcac gggggaggca gagggtttcc ctgccacagc ttgatagcgg 3661 ccgctcgagt ctagagggcc cgtttaaacc gctgatcagc ctcgactgtg ccttctagtt 3721 gccagccatc tgttgttgcc cgggcgcgat cgctgcccct cccccgtgcc ttccttgacc 3781 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt 3841 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat 3901 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa 3961 agaaccagca gatcgatctg catctatgtc gggtgcggag aaagaggtaa tgaaatggca 4021 ttatgggtat tatgggtctg cattaatgaa tcggccaacg gatcccggtg tgaaataccg 4081 cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac 4141 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata 4201 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa 4261 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct 4321 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 4381 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 4441 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca 4501 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 4561 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 4621 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 4681 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg 4741 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 4801 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag 4861 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 4921 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc 4981 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag 5041 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt 5101 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag 5161 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca 5221 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact 5281 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca 5341 gttaatagtt tgcgcaacgt tgttgaaaaa ggatcttcac ctagatcctt ttcacgtaga 5401 aagccagtcc gcagaaacgg tgctgacccc ggatgaatgt cagctactgg gctatctgga 5461 caagggaaaa cgcaagcgca aagagaaagc aggtagcttg cagtgggctt acatggcgat 5521 agctagactg ggcggtttta tggacagcaa gcgaaccgga attgccagct ggggcgccct 5581 ctggtaaggt tgggaagccc tgcaaagtaa actggatggc tttctcgccg ccaaggatct 5641 gatggcgcag gggatcaagc tctgatcaag agacaggatg aggatcgttt cgcatgattg 5701 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg 5761 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg 5821 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg 5881 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg 5941 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc 6001 tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc 6061 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc 6121 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc 6181 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg 6241 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct 6301 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt 6361 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc 6421 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt 6481 tcttctgaat tttgttaaaa tttttgttaa atcagctcat tttttaacca ataggccgaa 6541 atcggcaaca tcccttataa atcaaaagaa tagaccgcga tagggttgag tgttgttcca 6601 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc 6661 gtctatcagg gcgatggccc actacgtgaa ccatcaccca aatcaagttt tttgcggtcg 6721 aggtgccgta aagctctaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg 6781 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg 6841 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgcgcg cttaatgcgc 6901 cgctacaggg cgcgtccatt cgccattcag gatcgaatta attcttaatt aacatcatca 6961 ataatatacc tt //
LOCUS dna 6972 bp FEATURES Location/Qualifiers Other Gene 162..310 /gene="Encap other" Promoter 365..393 /gene="amp prom" misc_binding 457..462 /dbxref="REBASE:HpaI" misc_binding 672..677 /dbxref="REBASE:KpnI" misc_binding 678..683 /dbxref="REBASE:SacI" misc_binding 707..712 /dbxref="REBASE:EcoRI" ORF 730..3654 /sequence="ORF_1 rf(1)" misc_binding 1884..1889 /dbxref="REBASE:NruI" misc_binding 2221..2226 /dbxref="REBASE:EcoRV" misc_binding 3657..3664 /dbxref="REBASE:NotI" misc_binding 3676..3681 /dbxref="REBASE:ApaI" Rep_Origin 4284..4903 /gene="pBR322 origin" Promoter 5556..5605 /gene="NEOKAN prom" ORF 5694..6488 /sequence="ORF_2 rf(3)" Marker 5697..6485 /gene="NTP_II marker" Rep_Origin 6593..6898 /gene="f1 origin" BASE COUNT 1673 a 1754 c 1927 g 1618 t 0 others ORIGIN