pPM-C-HA

Vector Name	pPM-C-HA
VectorType	Protein Vector
Antibiotic Information	Kanamycin
Sequencing Primers	CMV sequencing primer 5`---CGC AAA TGG GCG GTA GGC GTG---3`
Additional Information	Original Vector: 6470bp Vector without Insert Cassette: 4765bp

***Note: Features may not be to scale, please refer to sequence or text map for detailed information.

***Note: The vector sequence shown below is representative of the parental vector only and does not include the gene of interest. The sequence of the gene can be found through the accession number link on the product page. The restriction sites within the cloning region may differ from the provided sequence.

1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt 61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa 121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg 181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt 241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg 301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag 361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta 481 gcgaaagctc agatctggat ctcccgatcc cctatggtcg actctcagta caatctgctc 541 tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta 601 gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa 661 tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg 721 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc 781 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa 841 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac 901 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca 961 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg 1021 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt 1081 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg 1141 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg 1201 gaaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat 1261 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctcccta tcagtgatag 1321 agatctccct atcagtgata gagatcgtcg acgagctcgt ttagtgaacc gtcagatcgc 1381 ctggagacgc catccacgct gttttgacct ccatagaagc tagcgatatc aacaagtttg 1441 tacaaaaaag ctgaacgaga aacgtaaaat gatataaata tcaatatatt aaattagatt 1501 ttgcataaaa aacagactac ataatactgt aaaacacaac atatccagtc actatggcgg 1561 ccgcattagg caccccaggc tttacacttt atgcttccgg ctcgtataat gtgtggattt 1621 tgagttagga tccgtcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca 1681 ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc 1741 agtcagttgc tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa 1801 agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc 1861 tgatgaatgc tcatccggaa ttccgtatgg caatgaaaga cggtgagctg gtgatatggg 1921 atagtgttca cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct 1981 ggagtgaata ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt 2041 gttacggtga aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct 2101 cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact 2161 tcttcgcccc cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc 2221 cgctggcgat tcaggttcat catgccgttt gtgatggctt ccatgtcggc agaatgctta 2281 atgaattaca acagtactgc gatgagtggc agggcggggc gtaaagatct ggatccggct 2341 tactaaaagc cagataacag tatgcgtatt tgcgcgctga tttttgcggt ataagaatat 2401 atactgatat gtatacccga agtatgtcaa aaagaggtat gctatgaagc agcgtattac 2461 agtgacagtt gacagcgaca gctatcagtt gctcaaggca tatatgatgt caatatctcc 2521 ggtctggtaa gcacaaccat gcagaatgaa gcccgtcgtc tgcgtgccga acgctggaaa 2581 gcggaaaatc aggaagggat ggctgaggtc gcccggttta ttgaaatgaa cggctctttt 2641 gctgacgaga acaggggctg gtgaaatgca gtttaaggtt tacacctata aaagagagag 2701 ccgttatcgt ctgtttgtgg atgtacagag tgatattatt gacacgcccg ggcgacggat 2761 ggtgatcccc ctggccagtg cacgtctgct gtcagataaa gtctcccgtg aactttaccc 2821 ggtggtgcat atcggggatg aaagctggcg catgatgacc accgatatgg ccagtgtgcc 2881 ggtctccgtt atcggggaag aagtggctga tctcagccac cgcgaaaatg acatcaaaaa 2941 cgccattaac ctgatgttct ggggaatata aatgtcaggc tcccttatac acagccagtc 3001 tgcaggtcga ccatagtgac tggatatgtt gtgttttaca gtattatgta gtctgttttt 3061 tatgcaaaat ctaatttaat atattgatat ttatatcatt ttacgtttct cgttcagctt 3121 tcttgtacaa agtggttgat atcctcgagt acccatacga cgtcccagac tacgcttgag 3181 tttaaaccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgttgcccg 3241 ggcgcgatcg ctgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg 3301 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc 3361 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg 3421 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagcaga tcgatctgca 3481 tctatgtcgg gtgcggagaa agaggtaatg aaatggcatt atgggtatta tgggtctgca 3541 ttaatgaatc ggccaacgga tcccggtgtg aaataccgca cagatgcgta aggagaaaat 3601 accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 3661 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg 3721 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg 3781 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 3841 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 3901 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 3961 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg 4021 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 4081 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 4141 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 4201 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc 4261 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 4321 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 4381 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 4441 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt 4501 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc 4561 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg 4621 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg 4681 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc 4741 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta 4801 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg 4861 ttgaaaaagg atcttcacct agatcctttt cacgtagaaa gccagtccgc agaaacggtg 4921 ctgaccccgg atgaatgtca gctactgggc tatctggaca agggaaaacg caagcgcaaa 4981 gagaaagcag gtagcttgca gtgggcttac atggcgatag ctagactggg cggttttatg 5041 gacagcaagc gaaccggaat tgccagctgg ggcgccctct ggtaaggttg ggaagccctg 5101 caaagtaaac tggatggctt tctcgccgcc aaggatctga tggcgcaggg gatcaagctc 5161 tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg 5221 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg 5281 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa 5341 gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc tatcgtggct 5401 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga 5461 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc 5521 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac 5581 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc 5641 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact 5701 gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga cccatggcga 5761 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg 5821 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga 5881 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga 5941 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgaattt tgttaaaatt 6001 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaacatc ccttataaat 6061 caaaagaata gaccgcgata gggttgagtg ttgttccagt ttggaacaag agtccactat 6121 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac 6181 tacgtgaacc atcacccaaa tcaagttttt tgcggtcgag gtgccgtaaa gctctaaatc 6241 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga 6301 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca 6361 cgctgcgcgt aaccaccaca cccgcgcgct taatgcgccg ctacagggcg cgtccattcg 6421 ccattcagga tcgaattaat tcttaattaa catcatcaat aatatacctt

                            LOCUS dna 6470 bp FEATURES Location/Qualifiers Other Gene 162..310 /gene="Encap other" Promoter 365..393 /gene="amp prom" misc_binding 457..462 /dbxref="REBASE:HpaI" misc_binding 691..696 /dbxref="REBASE:NruI" misc_binding 968..973 /dbxref="REBASE:NdeI" Regulatory_Seq 1305..1344 /gene="tetO reg" misc_binding 1557..1564 /dbxref="REBASE:NotI" Marker 1665..2324 /gene="CAT marker" ORF 1665..2324 /sequence="ORF_2 rf(3)" misc_binding 1878..1883 /dbxref="REBASE:EcoRI" Other Gene 2666..2971 /gene="ccdB other" misc_binding 3000..3005 /dbxref="REBASE:PstI" misc_binding 3144..3149 /dbxref="REBASE:XhoI" misc_binding 3470..3475 /dbxref="REBASE:ClaI" Rep_Origin 3782..4401 /gene="pBR322 origin" Promoter 5054..5103 /gene="NEOKAN prom" ORF 5192..5986 /sequence="ORF_1 rf(2)" Marker 5195..5983 /gene="NTP_II marker" Rep_Origin 6091..6396 /gene="f1 origin"

***Note: The text map shown below is representative of the parental vector only and does not include the gene of interest. The sequence of the gene can be found through the accession number link on the product page.

1 ttttggattgaagccaatatgataatgagggggtggagtttgtgacgtggcgcggggcgt 60 AAAACCTAACTTCGGTTATACTATTACTCCCCCACCTCAAACACTGCACCGCGCCCCGCA 61 gggaacggggcgggtgacgtagtagtgtggcggaagtgtgatgttgcaagtgtggcggaa 120 CCCTTGCCCCGCCCACTGCATCATCACACCGCCTTCACACTACAACGTTCACACCGCCTT Encap other(162,310)>>> | 121 cacatgtaagcgacggatgtggcaaaagtgacgtttttggtgtgcgccggtgtacacagg 180 GTGTACATTCGCTGCCTACACCGTTTTCACTGCAAAAACCACACGCGGCCACATGTGTCC 181 aagtgacaattttcgcgcggttttaggcggatgttgtagtaaatttgggcgtaaccgagt 240 TTCACTGTTAAAAGCGCGCCAAAATCCGCCTACAACATCATTTAAACCCGCATTGGCTCA 241 aagatttggccattttcgcgggaaaactgaataagaggaagtgaaatctgaataattttg 300 TTCTAAACCGGTAAAAGCGCCCTTTTGACTTATTCTCCTTCACTTTAGACTTATTAAAAC 301 tgttactcatagcgcgtaatacggcagacctcagcgctagattattgaagcatttatcag 360 ACAATGAGTATCGCGCATTATGCCGTCTGGAGTCGCGATCTAATAACTTCGTAAATAGTC amp prom(365,393)<<< | 361 ggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggg 420 CCAATAACAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTATCCC HpaI | 421 gttccgcgcacatttccccgaaaagtgccacctgacgttaactataacggtcctaaggta 480 CAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTGCAATTGATATTGCCAGGATTCCAT 481 gcgaaagctcagatctggatctcccgatcccctatggtcgactctcagtacaatctgctc 540 CGCTTTCGAGTCTAGACCTAGAGGGCTAGGGGATACCAGCTGAGAGTCATGTTAGACGAG 541 tgatgccgcatagttaagccagtatctgctccctgcttgtgtgttggaggtcgctgagta 600 ACTACGGCGTATCAATTCGGTCATAGACGAGGGACGAACACACAACCTCCAGCGACTCAT 601 gtgcgcgagcaaaatttaagctacaacaaggcaaggcttgaccgacaattgcatgaagaa 660 CACGCGCTCGTTTTAAATTCGATGTTGTTCCGTTCCGAACTGGCTGTTAACGTACTTCTT NruI | 661 tctgcttagggttaggcgttttgcgctgcttcgcgatgtacgggccagatatacgcgttg 720 AGACGAATCCCAATCCGCAAAACGCGACGAAGCGCTACATGCCCGGTCTATATGCGCAAC 721 acattgattattgactagttattaatagtaatcaattacggggtcattagttcatagccc 780 TGTAACTAATAACTGATCAATAATTATCATTAGTTAATGCCCCAGTAATCAAGTATCGGG 781 atatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaa 840 TATATACCTCAAGGCGCAATGTATTGAATGCCATTTACCGGGCGGACCGACTGGCGGGTT 841 cgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggac 900 GCTGGGGGCGGGTAACTGCAGTTATTACTGCATACAAGGGTATCATTGCGGTTATCCCTG 901 tttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatca 960 AAAGGTAACTGCAGTTACCCACCTCATAAATGCCATTTGACGGGTGAACCGTCATGTAGT NdeI | 961 agtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctg 1020 TCACATAGTATACGGTTCATGCGGGGGATAACTGCAGTTACTGCCATTTACCGGGCGGAC 1021 gcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtatt 1080 CGTAATACGGGTCATGTACTGGAATACCCTGAAAGGATGAACCGTCATGTAGATGCATAA 1081 agtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcg 1140 TCAGTAGCGATAATGGTACCACTACGCCAAAACCGTCATGTAGTTACCCGCACCTATCGC 1141 gtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttg 1200 CAAACTGAGTGCCCCTAAAGGTTCAGAGGTGGGGTAACTGCAGTTACCCTCAAACAAAAC 1201 gaaccaaaatcaacgggactttccaaaatgtcgtaacaactccgccccattgacgcaaat 1260 CTTGGTTTTAGTTGCCCTGAAAGGTTTTACAGCATTGTTGAGGCGGGGTAACTGCGTTTA tetO reg(1305,1344)>>> | 1261 gggcggtaggcgtgtacggtgggaggtctatataagcagagctctccctatcagtgatag 1320 CCCGCCATCCGCACATGCCACCCTCCAGATATATTCGTCTCGAGAGGGATAGTCACTATC 1321 agatctccctatcagtgatagagatcgtcgacgagctcgtttagtgaaccgtcagatcgc 1380 TCTAGAGGGATAGTCACTATCTCTAGCAGCTGCTCGAGCAAATCACTTGGCAGTCTAGCG 1381 ctggagacgccatccacgctgttttgacctccatagaagctagcgatatcaacaagtttg 1440 GACCTCTGCGGTAGGTGCGACAAAACTGGAGGTATCTTCGATCGCTATAGTTGTTCAAAC 1441 tacaaaaaagctgaacgagaaacgtaaaatgatataaatatcaatatattaaattagatt 1500 ATGTTTTTTCGACTTGCTCTTTGCATTTTACTATATTTATAGTTATATAATTTAATCTAA NotI | 1501 ttgcataaaaaacagactacataatactgtaaaacacaacatatccagtcactatggcgg 1560 AACGTATTTTTTGTCTGATGTATTATGACATTTTGTGTTGTATAGGTCAGTGATACCGCC 1561 ccgcattaggcaccccaggctttacactttatgcttccggctcgtataatgtgtggattt 1620 GGCGTAATCCGTGGGGTCCGAAATGTGAAATACGAAGGCCGAGCATATTACACACCTAAA ORF_2 rf(3)(1665,2324)>>> | CAT marker(1665,2324)>>> | 1621 tgagttaggatccgtcgagattttcaggagctaaggaagctaaaatggagaaaaaaatca 1680 ACTCAATCCTAGGCAGCTCTAAAAGTCCTCGATTCCTTCGATTTTACCTCTTTTTTTAGT 1681 ctggatataccaccgttgatatatcccaatggcatcgtaaagaacattttgaggcatttc 1740 GACCTATATGGTGGCAACTATATAGGGTTACCGTAGCATTTCTTGTAAAACTCCGTAAAG 1741 agtcagttgctcaatgtacctataaccagaccgttcagctggatattacggcctttttaa 1800 TCAGTCAACGAGTTACATGGATATTGGTCTGGCAAGTCGACCTATAATGCCGGAAAAATT 1801 agaccgtaaagaaaaataagcacaagttttatccggcctttattcacattcttgcccgcc 1860 TCTGGCATTTCTTTTTATTCGTGTTCAAAATAGGCCGGAAATAAGTGTAAGAACGGGCGG EcoRI | 1861 tgatgaatgctcatccggaattccgtatggcaatgaaagacggtgagctggtgatatggg 1920 ACTACTTACGAGTAGGCCTTAAGGCATACCGTTACTTTCTGCCACTCGACCACTATACCC 1921 atagtgttcacccttgttacaccgttttccatgagcaaactgaaacgttttcatcgctct 1980 TATCACAAGTGGGAACAATGTGGCAAAAGGTACTCGTTTGACTTTGCAAAAGTAGCGAGA 1981 ggagtgaataccacgacgatttccggcagtttctacacatatattcgcaagatgtggcgt 2040 CCTCACTTATGGTGCTGCTAAAGGCCGTCAAAGATGTGTATATAAGCGTTCTACACCGCA 2041 gttacggtgaaaacctggcctatttccctaaagggtttattgagaatatgtttttcgtct 2100 CAATGCCACTTTTGGACCGGATAAAGGGATTTCCCAAATAACTCTTATACAAAAAGCAGA 2101 cagccaatccctgggtgagtttcaccagttttgatttaaacgtggccaatatggacaact 2160 GTCGGTTAGGGACCCACTCAAAGTGGTCAAAACTAAATTTGCACCGGTTATACCTGTTGA 2161 tcttcgcccccgttttcaccatgggcaaatattatacgcaaggcgacaaggtgctgatgc 2220 AGAAGCGGGGGCAAAAGTGGTACCCGTTTATAATATGCGTTCCGCTGTTCCACGACTACG 2221 cgctggcgattcaggttcatcatgccgtttgtgatggcttccatgtcggcagaatgctta 2280 GCGACCGCTAAGTCCAAGTAGTACGGCAAACACTACCGAAGGTACAGCCGTCTTACGAAT 2281 atgaattacaacagtactgcgatgagtggcagggcggggcgtaaagatctggatccggct 2340 TACTTAATGTTGTCATGACGCTACTCACCGTCCCGCCCCGCATTTCTAGACCTAGGCCGA 2341 tactaaaagccagataacagtatgcgtatttgcgcgctgatttttgcggtataagaatat 2400 ATGATTTTCGGTCTATTGTCATACGCATAAACGCGCGACTAAAAACGCCATATTCTTATA 2401 atactgatatgtatacccgaagtatgtcaaaaagaggtatgctatgaagcagcgtattac 2460 TATGACTATACATATGGGCTTCATACAGTTTTTCTCCATACGATACTTCGTCGCATAATG 2461 agtgacagttgacagcgacagctatcagttgctcaaggcatatatgatgtcaatatctcc 2520 TCACTGTCAACTGTCGCTGTCGATAGTCAACGAGTTCCGTATATACTACAGTTATAGAGG 2521 ggtctggtaagcacaaccatgcagaatgaagcccgtcgtctgcgtgccgaacgctggaaa 2580 CCAGACCATTCGTGTTGGTACGTCTTACTTCGGGCAGCAGACGCACGGCTTGCGACCTTT 2581 gcggaaaatcaggaagggatggctgaggtcgcccggtttattgaaatgaacggctctttt 2640 CGCCTTTTAGTCCTTCCCTACCGACTCCAGCGGGCCAAATAACTTTACTTGCCGAGAAAA ccdB other(2666,2971)>>> | 2641 gctgacgagaacaggggctggtgaaatgcagtttaaggtttacacctataaaagagagag 2700 CGACTGCTCTTGTCCCCGACCACTTTACGTCAAATTCCAAATGTGGATATTTTCTCTCTC 2701 ccgttatcgtctgtttgtggatgtacagagtgatattattgacacgcccgggcgacggat 2760 GGCAATAGCAGACAAACACCTACATGTCTCACTATAATAACTGTGCGGGCCCGCTGCCTA 2761 ggtgatccccctggccagtgcacgtctgctgtcagataaagtctcccgtgaactttaccc 2820 CCACTAGGGGGACCGGTCACGTGCAGACGACAGTCTATTTCAGAGGGCACTTGAAATGGG 2821 ggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgatatggccagtgtgcc 2880 CCACCACGTATAGCCCCTACTTTCGACCGCGTACTACTGGTGGCTATACCGGTCACACGG 2881 ggtctccgttatcggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaa 2940 CCAGAGGCAATAGCCCCTTCTTCACCGACTAGAGTCGGTGGCGCTTTTACTGTAGTTTTT 2941 cgccattaacctgatgttctggggaatataaatgtcaggctcccttatacacagccagtc 3000 GCGGTAATTGGACTACAAGACCCCTTATATTTACAGTCCGAGGGAATATGTGTCGGTCAG PstI | 3001 tgcaggtcgaccatagtgactggatatgttgtgttttacagtattatgtagtctgttttt 3060 ACGTCCAGCTGGTATCACTGACCTATACAACACAAAATGTCATAATACATCAGACAAAAA 3061 tatgcaaaatctaatttaatatattgatatttatatcattttacgtttctcgttcagctt 3120 ATACGTTTTAGATTAAATTATATAACTATAAATATAGTAAAATGCAAAGAGCAAGTCGAA XhoI | 3121 tcttgtacaaagtggttgatatcctcgagtacccatacgacgtcccagactacgcttgag 3180 AGAACATGTTTCACCAACTATAGGAGCTCATGGGTATGCTGCAGGGTCTGATGCGAACTC 3181 tttaaaccgctgatcagcctcgactgtgccttctagttgccagccatctgttgttgcccg 3240 AAATTTGGCGACTAGTCGGAGCTGACACGGAAGATCAACGGTCGGTAGACAACAACGGGC 3241 ggcgcgatcgctgcccctcccccgtgccttccttgaccctggaaggtgccactcccactg 3300 CCGCGCTAGCGACGGGGAGGGGGCACGGAAGGAACTGGGACCTTCCACGGTGAGGGTGAC 3301 tcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattc 3360 AGGAAAGGATTATTTTACTCCTTTAACGTAGCGTAACAGACTCATCCACAGTAAGATAAG 3361 tggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatg 3420 ACCCCCCACCCCACCCCGTCCTGTCGTTCCCCCTCCTAACCCTTCTGTTATCGTCCGTAC ClaI | 3421 ctggggatgcggtgggctctatggcttctgaggcggaaagaaccagcagatcgatctgca 3480 GACCCCTACGCCACCCGAGATACCGAAGACTCCGCCTTTCTTGGTCGTCTAGCTAGACGT 3481 tctatgtcgggtgcggagaaagaggtaatgaaatggcattatgggtattatgggtctgca 3540 AGATACAGCCCACGCCTCTTTCTCCATTACTTTACCGTAATACCCATAATACCCAGACGT 3541 ttaatgaatcggccaacggatcccggtgtgaaataccgcacagatgcgtaaggagaaaat 3600 AATTACTTAGCCGGTTGCCTAGGGCCACACTTTATGGCGTGTCTACGCATTCCTCTTTTA 3601 accgcatcaggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggc 3660 TGGCGTAGTCCGCGAGAAGGCGAAGGAGCGAGTGACTGAGCGACGCGAGCCAGCAAGCCG 3661 tgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcagggg 3720 ACGCCGCTCGCCATAGTCGAGTGAGTTTCCGCCATTATGCCAATAGGTGTCTTAGTCCCC 3721 ataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaagg 3780 TATTGCGTCCTTTCTTGTACACTCGTTTTCCGGTCGTTTTCCGGTCCTTGGCATTTTTCC pBR322 origin(3782,4401)<<< | 3781 ccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgac 3840 GGCGCAACGACCGCAAAAAGGTATCCGAGGCGGGGGGACTGCTCGTAGTGTTTTTAGCTG 3841 gctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctg 3900 CGAGTTCAGTCTCCACCGCTTTGGGCTGTCCTGATATTTCTATGGTCCGCAAAGGGGGAC 3901 gaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcct 3960 CTTCGAGGGAGCACGCGAGAGGACAAGGCTGGGACGGCGAATGGCCTATGGACAGGCGGA 3961 ttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcgg 4020 AAGAGGGAAGCCCTTCGCACCGCGAAAGAGTATCGAGTGCGACATCCATAGAGTCAAGCC 4021 tgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgct 4080 ACATCCAGCAAGCGAGGTTCGACCCGACACACGTGCTTGGGGGGCAAGTCGGGCTGGCGA 4081 gcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccac 4140 CGCGGAATAGGCCATTGATAGCAGAACTCAGGTTGGGCCATTCTGTGCTGAATAGCGGTG 4141 tggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagt 4200 ACCGTCGTCGGTGACCATTGTCCTAATCGTCTCGCTCCATACATCCGCCACGATGTCTCA 4201 tcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctc 4260 AGAACTTCACCACCGGATTGATGCCGATGTGATCTTCCTGTCATAAACCATAGACGCGAG 4261 tgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaacca 4320 ACGACTTCGGTCAATGGAAGCCTTTTTCTCAACCATCGAGAACTAGGCCGTTTGTTTGGT 4321 ccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggat 4380 GGCGACCATCGCCACCAAAAAAACAAACGTTCGTCGTCTAATGCGCGTCTTTTTTTCCTA 4381 ctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcac 4440 GAGTTCTTCTAGGAAACTAGAAAAGATGCCCCAGACTGCGAGTCACCTTGCTTTTGAGTG 4441 gttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaatt 4500 CAATTCCCTAAAACCAGTACTCTAATAGTTTTTCCTAGAAGTGGATCTAGGAAAATTTAA 4501 aaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttacc 4560 TTTTTACTTCAAAATTTAGTTAGATTTCATATATACTCATTTGAACCAGACTGTCAATGG 4561 aatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttg 4620 TTACGAATTAGTCACTCCGTGGATAGAGTCGCTAGACAGATAAAGCAAGTAGGTATCAAC 4621 cctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtg 4680 GGACTGAGGGGCAGCACATCTATTGATGCTATGCCCTCCCGAATGGTAGACCGGGGTCAC 4681 ctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagc 4740 GACGTTACTATGGCGCTCTGGGTGCGAGTGGCCGAGGTCTAAATAGTCGTTATTTGGTCG 4741 cagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtcta 4800 GTCGGCCTTCCCGGCTCGCGTCTTCACCAGGACGTTGAAATAGGCGGAGGTAGGTCAGAT 4801 ttaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttg 4860 AATTAACAACGGCCCTTCGATCTCATTCATCAAGCGGTCAATTATCAAACGCGTTGCAAC 4861 ttgaaaaaggatcttcacctagatccttttcacgtagaaagccagtccgcagaaacggtg 4920 AACTTTTTCCTAGAAGTGGATCTAGGAAAAGTGCATCTTTCGGTCAGGCGTCTTTGCCAC 4921 ctgaccccggatgaatgtcagctactgggctatctggacaagggaaaacgcaagcgcaaa 4980 GACTGGGGCCTACTTACAGTCGATGACCCGATAGACCTGTTCCCTTTTGCGTTCGCGTTT 4981 gagaaagcaggtagcttgcagtgggcttacatggcgatagctagactgggcggttttatg 5040 CTCTTTCGTCCATCGAACGTCACCCGAATGTACCGCTATCGATCTGACCCGCCAAAATAC NEOKAN prom(5054,5103)>>> | 5041 gacagcaagcgaaccggaattgccagctggggcgccctctggtaaggttgggaagccctg 5100 CTGTCGTTCGCTTGGCCTTAACGGTCGACCCCGCGGGAGACCATTCCAACCCTTCGGGAC 5101 caaagtaaactggatggctttctcgccgccaaggatctgatggcgcaggggatcaagctc 5160 GTTTCATTTGACCTACCGAAAGAGCGGCGGTTCCTAGACTACCGCGTCCCCTAGTTCGAG NTP_II marker(5195,5983)>>> | ORF_1 rf(2)(5192,5986)>>> | | 5161 tgatcaagagacaggatgaggatcgtttcgcatgattgaacaagatggattgcacgcagg 5220 ACTAGTTCTCTGTCCTACTCCTAGCAAAGCGTACTAACTTGTTCTACCTAACGTGCGTCC 5221 ttctccggccgcttgggtggagaggctattcggctatgactgggcacaacagacaatcgg 5280 AAGAGGCCGGCGAACCCACCTCTCCGATAAGCCGATACTGACCCGTGTTGTCTGTTAGCC 5281 ctgctctgatgccgccgtgttccggctgtcagcgcaggggcgcccggttctttttgtcaa 5340 GACGAGACTACGGCGGCACAAGGCCGACAGTCGCGTCCCCGCGGGCCAAGAAAAACAGTT 5341 gaccgacctgtccggtgccctgaatgaactgcaagacgaggcagcgcggctatcgtggct 5400 CTGGCTGGACAGGCCACGGGACTTACTTGACGTTCTGCTCCGTCGCGCCGATAGCACCGA 5401 ggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactgaagcgggaaggga 5460 CCGGTGCTGCCCGCAAGGAACGCGTCGACACGAGCTGCAACAGTGACTTCGCCCTTCCCT 5461 ctggctgctattgggcgaagtgccggggcaggatctcctgtcatctcaccttgctcctgc 5520 GACCGACGATAACCCGCTTCACGGCCCCGTCCTAGAGGACAGTAGAGTGGAACGAGGACG 5521 cgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttgatccggctac 5580 GCTCTTTCATAGGTAGTACCGACTACGTTACGCCGCCGACGTATGCGAACTAGGCCGATG 5581 ctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaagc 5640 GACGGGTAAGCTGGTGGTTCGCTTTGTAGCGTAGCTCGCTCGTGCATGAGCCTACCTTCG 5641 cggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgccagccgaact 5700 GCCAGAACAGCTAGTCCTACTAGACCTGCTTCTCGTAGTCCCCGAGCGCGGTCGGCTTGA 5701 gttcgccaggctcaaggcgagcatgcccgacggcgaggatctcgtcgtgacccatggcga 5760 CAAGCGGTCCGAGTTCCGCTCGTACGGGCTGCCGCTCCTAGAGCAGCACTGGGTACCGCT 5761 tgcctgcttgccgaatatcatggtggaaaatggccgcttttctggattcatcgactgtgg 5820 ACGGACGAACGGCTTATAGTACCACCTTTTACCGGCGAAAAGACCTAAGTAGCTGACACC 5821 ccggctgggtgtggcggaccgctatcaggacatagcgttggctacccgtgatattgctga 5880 GGCCGACCCACACCGCCTGGCGATAGTCCTGTATCGCAACCGATGGGCACTATAACGACT 5881 agagcttggcggcgaatgggctgaccgcttcctcgtgctttacggtatcgccgctcccga 5940 TCTCGAACCGCCGCTTACCCGACTGGCGAAGGAGCACGAAATGCCATAGCGGCGAGGGCT 5941 ttcgcagcgcatcgccttctatcgccttcttgacgagttcttctgaattttgttaaaatt 6000 AAGCGTCGCGTAGCGGAAGATAGCGGAAGAACTGCTCAAGAAGACTTAAAACAATTTTAA 6001 tttgttaaatcagctcattttttaaccaataggccgaaatcggcaacatcccttataaat 6060 AAACAATTTAGTCGAGTAAAAAATTGGTTATCCGGCTTTAGCCGTTGTAGGGAATATTTA f1 origin(6091,6396)<<< | 6061 caaaagaatagaccgcgatagggttgagtgttgttccagtttggaacaagagtccactat 6120 GTTTTCTTATCTGGCGCTATCCCAACTCACAACAAGGTCAAACCTTGTTCTCAGGTGATA 6121 taaagaacgtggactccaacgtcaaagggcgaaaaaccgtctatcagggcgatggcccac 6180 ATTTCTTGCACCTGAGGTTGCAGTTTCCCGCTTTTTGGCAGATAGTCCCGCTACCGGGTG 6181 tacgtgaaccatcacccaaatcaagttttttgcggtcgaggtgccgtaaagctctaaatc 6240 ATGCACTTGGTAGTGGGTTTAGTTCAAAAAACGCCAGCTCCACGGCATTTCGAGATTTAG 6241 ggaaccctaaagggagcccccgatttagagcttgacggggaaagccggcgaacgtggcga 6300 CCTTGGGATTTCCCTCGGGGGCTAAATCTCGAACTGCCCCTTTCGGCCGCTTGCACCGCT 6301 gaaaggaagggaagaaagcgaaaggagcgggcgctagggcgctggcaagtgtagcggtca 6360 CTTTCCTTCCCTTCTTTCGCTTTCCTCGCCCGCGATCCCGCGACCGTTCACATCGCCAGT 6361 cgctgcgcgtaaccaccacacccgcgcgcttaatgcgccgctacagggcgcgtccattcg 6420 GCGACGCGCATTGGTGGTGTGGGCGCGCGAATTACGCGGCGATGTCCCGCGCAGGTAAGC 6421 ccattcaggatcgaattaattcttaattaacatcatcaataatatacctt 6470 GGTAAGTCCTAGCTTAATTAAGAATTAATTGTAGTAGTTATTATATGGAA