pNV-sgRNA-GFP
| Vector Name | pNV-sgRNA-GFP |
| VectorType | Non-viral sgRNA expression vector (compatible with spCas9 systems) |
| Antibiotic Information | Bacterial: Ampicillin |
| Sequencing Primers | U6 Forward Primer: 5'--GAGGGCCTATTTCCCATGATTCC--3' |
| Additional Information | No Additional Information |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acctcgcgaa
421 tgcatctaga tatccctagg gggtagggga ggcgcttttc ccaaggcagt ctggagcatg
481 cgctttagca gccccgctgg gcacttggcg ctacacaagt ggcctctggc ctcgcacaca
541 ttccacatcc accggtaggc gccaaccggc tccgttcttt ggtggcccct tcgcgccacc
601 ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac
661 gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca ccgctgagca
721 atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct tcgctttctg
781 ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg
841 ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc
901 tgccgcgctg ttctcctctt cctcatctcc gggcctttcg gctagcacct cgccaccatg
961 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
1021 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
1081 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
1141 gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag
1201 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
1261 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
1321 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
1381 ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc
1441 atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac
1501 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
1561 ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg
1621 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaactc
1681 gagctgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
1741 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
1801 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
1861 attgggaaga gaatagcagg catgctgggg acaattggag ggcctatttc ccatgattcc
1921 ttcatatttg catatacgat acaaggctgt tagagagata attagaatta atttgactgt
1981 aaacacaaag atattagtac aaaatacgtg acgtagaaag taataatttc ttgggtagtt
2041 tgcagtttta aaattatgtt ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta
2101 tttcgatttc ttgggtttat atatcttgtg gaaaggacga aacaccggag tcttcaccgg
2161 tgaagacgcg ttttagagct agaaatagca agttaaaata aggctagtcc gttatcaact
2221 tgaaaaagtg gcaccgagtc ggtgcttttt tttgatatcg gatcccgggc ccgtcgactg
2281 cagaggcctg catgcaagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
2341 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
2401 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
2461 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
2521 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
2581 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
2641 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
2701 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
2761 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
2821 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
2881 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
2941 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
3001 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
3061 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
3121 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
3181 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
3241 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
3301 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
3361 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
3421 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
3481 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
3541 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
3601 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
3661 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
3721 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
3781 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
3841 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
3901 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
3961 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
4021 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
4081 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
4141 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
4201 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
4261 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
4321 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
4381 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
4441 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
4501 ctataaaaat aggcgtatca cgaggccctt tcgtc
LOCUS dna 4535 bp
FEATURES Location/Qualifiers
Reporter 239..398
/gene="lacZ_a reporter"
misc_binding 396..401
/dbxref="REBASE:EcoRI"
misc_binding 396..401
/dbxref="REBASE:ApoI"
misc_binding 402..407
/dbxref="REBASE:SacI"
misc_binding 408..413
/dbxref="REBASE:KpnI"
misc_binding 414..419
/dbxref="REBASE:NruI"
misc_binding 425..430
/dbxref="REBASE:XbaI"
misc_binding 956..961
/dbxref="REBASE:NcoI"
Reporter 958..1674
/gene="EGFP reporter"
ORF 958..1677
/sequence="ORF_1 rf(1)"
misc_binding 1678..1683
/dbxref="REBASE:XhoI"
misc_binding 2260..2265
/dbxref="REBASE:BamHI"
misc_binding 2264..2269
/dbxref="REBASE:SmaI"
misc_binding 2264..2269
/dbxref="REBASE:XmaI"
misc_binding 2267..2272
/dbxref="REBASE:ApaI"
misc_binding 2273..2278
/dbxref="REBASE:HincII"
misc_binding 2273..2278
/dbxref="REBASE:AccI"
misc_binding 2278..2283
/dbxref="REBASE:PstI"
misc_binding 2296..2301
/dbxref="REBASE:HindIII"
Promoter 2363..2392
/gene="lac prom"
Rep_Origin 2701..3320
/gene="pBR322 origin"
Marker 3475..4335
/gene="amp marker"
ORF 3475..4335
/sequence="ORF_2 rf(6)"
Promoter 4377..4405
/gene="amp prom"
1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acctcgcgaa
421 tgcatctaga tatccctagg gggtagggga ggcgcttttc ccaaggcagt ctggagcatg
481 cgctttagca gccccgctgg gcacttggcg ctacacaagt ggcctctggc ctcgcacaca
541 ttccacatcc accggtaggc gccaaccggc tccgttcttt ggtggcccct tcgcgccacc
601 ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac
661 gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca ccgctgagca
721 atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct tcgctttctg
781 ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg
841 ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc
901 tgccgcgctg ttctcctctt cctcatctcc gggcctttcg gctagcacct cgccaccatg
961 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
1021 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
1081 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
1141 gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag
1201 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
1261 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
1321 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
1381 ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc
1441 atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac
1501 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
1561 ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg
1621 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaactc
1681 gagctgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
1741 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
1801 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
1861 attgggaaga gaatagcagg catgctgggg acaattggag ggcctatttc ccatgattcc
1921 ttcatatttg catatacgat acaaggctgt tagagagata attagaatta atttgactgt
1981 aaacacaaag atattagtac aaaatacgtg acgtagaaag taataatttc ttgggtagtt
2041 tgcagtttta aaattatgtt ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta
2101 tttcgatttc ttgggtttat atatcttgtg gaaaggacga aacaccggag tcttcaccgg
2161 tgaagacgcg ttttagagct agaaatagca agttaaaata aggctagtcc gttatcaact
2221 tgaaaaagtg gcaccgagtc ggtgcttttt tttgatatcg gatcccgggc ccgtcgactg
2281 cagaggcctg catgcaagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
2341 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
2401 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
2461 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
2521 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
2581 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
2641 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
2701 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
2761 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
2821 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
2881 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
2941 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
3001 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
3061 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
3121 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
3181 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
3241 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
3301 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
3361 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
3421 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
3481 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
3541 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
3601 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
3661 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
3721 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
3781 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
3841 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
3901 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
3961 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
4021 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
4081 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
4141 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
4201 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
4261 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
4321 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
4381 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
4441 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
4501 ctataaaaat aggcgtatca cgaggccctt tcgtc
***Note: The text map shown below is representative of the parental
vector only and does not include the gene of interest. The sequence of the gene
can be found through the accession number link on the product page.
1 tcgcgcgtttcggtgatgacggtgaaaacctctgacacatgcagctcccggagacggtca 60
AGCGCGCAAAGCCACTACTGCCACTTTTGGAGACTGTGTACGTCGAGGGCCTCTGCCAGT
61 cagcttgtctgtaagcggatgccgggagcagacaagcccgtcagggcgcgtcagcgggtg 120
GTCGAACAGACATTCGCCTACGGCCCTCGTCTGTTCGGGCAGTCCCGCGCAGTCGCCCAC
121 ttggcgggtgtcggggctggcttaactatgcggcatcagagcagattgtactgagagtgc 180
AACCGCCCACAGCCCCGACCGAATTGATACGCCGTAGTCTCGTCTAACATGACTCTCACG
lacZ_a reporter(239,398)<<<
|
181 accatatgcggtgtgaaataccgcacagatgcgtaaggagaaaataccgcatcaggcgcc 240
TGGTATACGCCACACTTTATGGCGTGTCTACGCATTCCTCTTTTATGGCGTAGTCCGCGG
241 attcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgctat 300
TAAGCGGTAAGTCCGACGCGTTGACAACCCTTCCCGCTAGCCACGCCCGGAGAAGCGATA
301 tacgccagctggcgaaagggggatgtgctgcaaggcgattaagttgggtaacgccagggt 360
ATGCGGTCGACCGCTTTCCCCCTACACGACGTTCCGCTAATTCAACCCATTGCGGTCCCA
ApoI NruI
| |
EcoRI SacI KpnI
| | | |
361 tttcccagtcacgacgttgtaaaacgacggccagtgaattcgagctcggtacctcgcgaa 420
AAAGGGTCAGTGCTGCAACATTTTGCTGCCGGTCACTTAAGCTCGAGCCATGGAGCGCTT
XbaI
|
421 tgcatctagatatccctagggggtaggggaggcgcttttcccaaggcagtctggagcatg 480
ACGTAGATCTATAGGGATCCCCCATCCCCTCCGCGAAAAGGGTTCCGTCAGACCTCGTAC
481 cgctttagcagccccgctgggcacttggcgctacacaagtggcctctggcctcgcacaca 540
GCGAAATCGTCGGGGCGACCCGTGAACCGCGATGTGTTCACCGGAGACCGGAGCGTGTGT
541 ttccacatccaccggtaggcgccaaccggctccgttctttggtggccccttcgcgccacc 600
AAGGTGTAGGTGGCCATCCGCGGTTGGCCGAGGCAAGAAACCACCGGGGAAGCGCGGTGG
601 ttctactcctcccctagtcaggaagttcccccccgccccgcagctcgcgtcgtgcaggac 660
AAGATGAGGAGGGGATCAGTCCTTCAAGGGGGGGCGGGGCGTCGAGCGCAGCACGTCCTG
661 gtgacaaatggaagtagcacgtctcactagtctcgtgcagatggacagcaccgctgagca 720
CACTGTTTACCTTCATCGTGCAGAGTGATCAGAGCACGTCTACCTGTCGTGGCGACTCGT
721 atggaagcgggtaggcctttggggcagcggccaatagcagctttgctccttcgctttctg 780
TACCTTCGCCCATCCGGAAACCCCGTCGCCGGTTATCGTCGAAACGAGGAAGCGAAAGAC
781 ggctcagaggctgggaaggggtgggtccgggggcgggctcaggggcgggctcaggggcgg 840
CCGAGTCTCCGACCCTTCCCCACCCAGGCCCCCGCCCGAGTCCCCGCCCGAGTCCCCGCC
841 ggcgggcgcccgaaggtcctccggaggcccggcattctgcacgcttcaaaagcgcacgtc 900
CCGCCCGCGGGCTTCCAGGAGGCCTCCGGGCCGTAAGACGTGCGAAGTTTTCGCGTGCAG
ORF_1 rf(1)(958,1677)>>>
|
EGFP reporter(958,1674)>>>
|
NcoI
||
901 tgccgcgctgttctcctcttcctcatctccgggcctttcggctagcacctcgccaccatg 960
ACGGCGCGACAAGAGGAGAAGGAGTAGAGGCCCGGAAAGCCGATCGTGGAGCGGTGGTAC
961 gtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggc 1020
CACTCGTTCCCGCTCCTCGACAAGTGGCCCCACCACGGGTAGGACCAGCTCGACCTGCCG
1021 gacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggc 1080
CTGCATTTGCCGGTGTTCAAGTCGCACAGGCCGCTCCCGCTCCCGCTACGGTGGATGCCG
1081 aagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctc 1140
TTCGACTGGGACTTCAAGTAGACGTGGTGGCCGTTCGACGGGCACGGGACCGGGTGGGAG
1141 gtgaccaccctgacctacggcgtgcagtgcttcagccgctaccccgaccacatgaagcag 1200
CACTGGTGGGACTGGATGCCGCACGTCACGAAGTCGGCGATGGGGCTGGTGTACTTCGTC
1201 cacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttc 1260
GTGCTGAAGAAGTTCAGGCGGTACGGGCTTCCGATGCAGGTCCTCGCGTGGTAGAAGAAG
1261 aaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtg 1320
TTCCTGCTGCCGTTGATGTTCTGGGCGCGGCTCCACTTCAAGCTCCCGCTGTGGGACCAC
1321 aaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaag 1380
TTGGCGTAGCTCGACTTCCCGTAGCTGAAGTTCCTCCTGCCGTTGTAGGACCCCGTGTTC
1381 ctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggc 1440
GACCTCATGTTGATGTTGTCGGTGTTGCAGATATAGTACCGGCTGTTCGTCTTCTTGCCG
1441 atcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgac 1500
TAGTTCCACTTGAAGTTCTAGGCGGTGTTGTAGCTCCTGCCGTCGCACGTCGAGCGGCTG
1501 cactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactac 1560
GTGATGGTCGTCTTGTGGGGGTAGCCGCTGCCGGGGCACGACGACGGGCTGTTGGTGATG
1561 ctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctg 1620
GACTCGTGGGTCAGGCGGGACTCGTTTCTGGGGTTGCTCTTCGCGCTAGTGTACCAGGAC
XhoI
|
1621 ctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaactc 1680
GACCTCAAGCACTGGCGGCGGCCCTAGTGAGAGCCGTACCTGCTCGACATGTTCATTGAG
1681 gagctgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttga 1740
CTCGACACGGAAGATCAACGGTCGGTAGACAACAAACGGGGAGGGGGCACGGAAGGAACT
1741 ccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcatt 1800
GGGACCTTCCACGGTGAGGGTGACAGGAAAGGATTATTTTACTCCTTTAACGTAGCGTAA
1801 gtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggagg 1860
CAGACTCATCCACAGTAAGATAAGACCCCCCACCCCACCCCGTCCTGTCGTTCCCCCTCC
1861 attgggaagagaatagcaggcatgctggggacaattggagggcctatttcccatgattcc 1920
TAACCCTTCTCTTATCGTCCGTACGACCCCTGTTAACCTCCCGGATAAAGGGTACTAAGG
1921 ttcatatttgcatatacgatacaaggctgttagagagataattagaattaatttgactgt 1980
AAGTATAAACGTATATGCTATGTTCCGACAATCTCTCTATTAATCTTAATTAAACTGACA
1981 aaacacaaagatattagtacaaaatacgtgacgtagaaagtaataatttcttgggtagtt 2040
TTTGTGTTTCTATAATCATGTTTTATGCACTGCATCTTTCATTATTAAAGAACCCATCAA
2041 tgcagttttaaaattatgttttaaaatggactatcatatgcttaccgtaacttgaaagta 2100
ACGTCAAAATTTTAATACAAAATTTTACCTGATAGTATACGAATGGCATTGAACTTTCAT
2101 tttcgatttcttgggtttatatatcttgtggaaaggacgaaacaccggagtcttcaccgg 2160
AAAGCTAAAGAACCCAAATATATAGAACACCTTTCCTGCTTTGTGGCCTCAGAAGTGGCC
2161 tgaagacgcgttttagagctagaaatagcaagttaaaataaggctagtccgttatcaact 2220
ACTTCTGCGCAAAATCTCGATCTTTATCGTTCAATTTTATTCCGATCAGGCAATAGTTGA
HincII
|
XmaI AccI
| ||
BamHI SmaI ApaI
| | | | ||
2221 tgaaaaagtggcaccgagtcggtgcttttttttgatatcggatcccgggcccgtcgactg 2280
ACTTTTTCACCGTGGCTCAGCCACGAAAAAAAACTATAGCCTAGGGCCCGGGCAGCTGAC
PstI HindIII
| |
2281 cagaggcctgcatgcaagcttggcgtaatcatggtcatagctgtttcctgtgtgaaattg 2340
GTCTCCGGACGTACGTTCGAACCGCATTAGTACCAGTATCGACAAAGGACACACTTTAAC
lac prom(2363,2392)<<<
|
2341 ttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctgggg 2400
AATAGGCGAGTGTTAAGGTGTGTTGTATGCTCGGCCTTCGTATTTCACATTTCGGACCCC
2401 tgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtc 2460
ACGGATTACTCACTCGATTGAGTGTAATTAACGCAACGCGAGTGACGGGCGAAAGGTCAG
2461 gggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggttt 2520
CCCTTTGGACAGCACGGTCGACGTAATTACTTAGCCGGTTGCGCGCCCCTCTCCGCCAAA
2521 gcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggct 2580
CGCATAACCCGCGAGAAGGCGAAGGAGCGAGTGACTGAGCGACGCGAGCCAGCAAGCCGA
2581 gcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcagggga 2640
CGCCGCTCGCCATAGTCGAGTGAGTTTCCGCCATTATGCCAATAGGTGTCTTAGTCCCCT
2641 taacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggc 2700
ATTGCGTCCTTTCTTGTACACTCGTTTTCCGGTCGTTTTCCGGTCCTTGGCATTTTTCCG
pBR322 origin(2701,3320)<<<
|
2701 cgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacg 2760
GCGCAACGACCGCAAAAAGGTATCCGAGGCGGGGGGACTGCTCGTAGTGTTTTTAGCTGC
2761 ctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctgg 2820
GAGTTCAGTCTCCACCGCTTTGGGCTGTCCTGATATTTCTATGGTCCGCAAAGGGGGACC
2821 aagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctt 2880
TTCGAGGGAGCACGCGAGAGGACAAGGCTGGGACGGCGAATGGCCTATGGACAGGCGGAA
2881 tctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggt 2940
AGAGGGAAGCCCTTCGCACCGCGAAAGAGTATCGAGTGCGACATCCATAGAGTCAAGCCA
2941 gtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctg 3000
CATCCAGCAAGCGAGGTTCGACCCGACACACGTGCTTGGGGGGCAAGTCGGGCTGGCGAC
3001 cgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccact 3060
GCGGAATAGGCCATTGATAGCAGAACTCAGGTTGGGCCATTCTGTGCTGAATAGCGGTGA
3061 ggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagtt 3120
CCGTCGTCGGTGACCATTGTCCTAATCGTCTCGCTCCATACATCCGCCACGATGTCTCAA
3121 cttgaagtggtggcctaactacggctacactagaagaacagtatttggtatctgcgctct 3180
GAACTTCACCACCGGATTGATGCCGATGTGATCTTCTTGTCATAAACCATAGACGCGAGA
3181 gctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccac 3240
CGACTTCGGTCAATGGAAGCCTTTTTCTCAACCATCGAGAACTAGGCCGTTTGTTTGGTG
3241 cgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatc 3300
GCGACCATCGCCACCAAAAAAACAAACGTTCGTCGTCTAATGCGCGTCTTTTTTTCCTAG
3301 tcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacg 3360
AGTTCTTCTAGGAAACTAGAAAAGATGCCCCAGACTGCGAGTCACCTTGCTTTTGAGTGC
3361 ttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaatta 3420
AATTCCCTAAAACCAGTACTCTAATAGTTTTTCCTAGAAGTGGATCTAGGAAAATTTAAT
ORF_2 rf(6)(3475,4335)<<<
|
amp marker(3475,4335)<<<
|
3421 aaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttacca 3480
TTTTACTTCAAAATTTAGTTAGATTTCATATATACTCATTTGAACCAGACTGTCAATGGT
3481 atgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgc 3540
TACGAATTAGTCACTCCGTGGATAGAGTCGCTAGACAGATAAAGCAAGTAGGTATCAACG
3541 ctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgc 3600
GACTGAGGGGCAGCACATCTATTGATGCTATGCCCTCCCGAATGGTAGACCGGGGTCACG
3601 tgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagcc 3660
ACGTTACTATGGCGCTCTGGGTGCGAGTGGCCGAGGTCTAAATAGTCGTTATTTGGTCGG
3661 agccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctat 3720
TCGGCCTTCCCGGCTCGCGTCTTCACCAGGACGTTGAAATAGGCGGAGGTAGGTCAGATA
3721 taattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgt 3780
ATTAACAACGGCCCTTCGATCTCATTCATCAAGCGGTCAATTATCAAACGCGTTGCAACA
3781 tgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctc 3840
ACGGTAACGATGTCCGTAGCACCACAGTGCGAGCAGCAAACCATACCGAAGTAAGTCGAG
3841 cggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttag 3900
GCCAAGGGTTGCTAGTTCCGCTCAATGTACTAGGGGGTACAACACGTTTTTTCGCCAATC
3901 ctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggt 3960
GAGGAAGCCAGGAGGCTAGCAACAGTCTTCATTCAACCGGCGTCACAATAGTGAGTACCA
3961 tatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgac 4020
ATACCGTCGTGACGTATTAAGAGAATGACAGTACGGTAGGCATTCTACGAAAAGACACTG
4021 tggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttg 4080
ACCACTCATGAGTTGGTTCAGTAAGACTCTTATCACATACGCCGCTGGCTCAACGAGAAC
4081 cccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcat 4140
GGGCCGCAGTTATGCCCTATTATGGCGCGGTGTATCGTCTTGAAATTTTCACGAGTAGTA
4141 tggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttc 4200
ACCTTTTGCAAGAAGCCCCGCTTTTGAGAGTTCCTAGAATGGCGACAACTCTAGGTCAAG
4201 gatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttc 4260
CTACATTGGGTGAGCACGTGGGTTGACTAGAAGTCGTAGAAAATGAAAGTGGTCGCAAAG
4261 tgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaa 4320
ACCCACTCGTTTTTGTCCTTCCGTTTTACGGCGTTTTTTCCCTTATTCCCGCTGTGCCTT
amp prom(4377,4405)<<<
|
4321 atgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattg 4380
TACAACTTATGAGTATGAGAAGGAAAAAGTTATAATAACTTCGTAAATAGTCCCAATAAC
4381 tctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcg 4440
AGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTATCCCCAAGGCGC
4441 cacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaac 4500
GTGTAAAGGGGCTTTTCACGGTGGACTGCAGATTCTTTGGTAATAATAGTACTGTAATTG
4501 ctataaaaataggcgtatcacgaggccctttcgtc 4535
GATATTTTTATCCGCATAGTGCTCCGGGAAAGCAG
LOCUS dna 4535 bp
FEATURES Location/Qualifiers
Reporter 239..398
/gene="lacZ_a reporter"
misc_binding 396..401
/dbxref="REBASE:EcoRI"
misc_binding 396..401
/dbxref="REBASE:ApoI"
misc_binding 402..407
/dbxref="REBASE:SacI"
misc_binding 408..413
/dbxref="REBASE:KpnI"
misc_binding 414..419
/dbxref="REBASE:NruI"
misc_binding 425..430
/dbxref="REBASE:XbaI"
misc_binding 956..961
/dbxref="REBASE:NcoI"
Reporter 958..1674
/gene="EGFP reporter"
ORF 958..1677
/sequence="ORF_1 rf(1)"
misc_binding 1678..1683
/dbxref="REBASE:XhoI"
misc_binding 2260..2265
/dbxref="REBASE:BamHI"
misc_binding 2264..2269
/dbxref="REBASE:SmaI"
misc_binding 2264..2269
/dbxref="REBASE:XmaI"
misc_binding 2267..2272
/dbxref="REBASE:ApaI"
misc_binding 2273..2278
/dbxref="REBASE:HincII"
misc_binding 2273..2278
/dbxref="REBASE:AccI"
misc_binding 2278..2283
/dbxref="REBASE:PstI"
misc_binding 2296..2301
/dbxref="REBASE:HindIII"
Promoter 2363..2392
/gene="lac prom"
Rep_Origin 2701..3320
/gene="pBR322 origin"
Marker 3475..4335
/gene="amp marker"
ORF 3475..4335
/sequence="ORF_2 rf(6)"
Promoter 4377..4405
/gene="amp prom"