pLuc
| Vector Name | pLuc |
| VectorType | Plasmid |
| Antibiotic Information | Kanamycin |
| Sequencing Primers | Sequencing Primer (471-490) 5`---TCCTAAGGTAGCGAAAGCTC---3` |
| Additional Information | No Additional Information |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt 60
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa 120
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg 180
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt 240
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg 300
301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag 360
361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 420
421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta 480
481 gcgaaagctc agatctggat ctcccgatcc cctatggtcg actctcagta caatctgctc 540
541 tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta 600
601 gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcttaagatt 660
661 taaataagct tggtaccgag ctcggatcca ctagtccagt gtggtggaat tctgcagata 720
721 tccagcacag tggcggccgc tcgagttcta gagggtatat aatggaagct cgaattccag 780
781 cttggcattc cggtactgtt ggtaaaaagc ttggcattcc ggtactgttg gtaaagccac 840
841 catggaagac gccaaaaaca taaagaaagg cccggcgcca ttctatccgc tggaagatgg 900
901 aaccgctgga gagcaactgc ataaggctat gaagagatac gccctggttc ctggaacaat 960
961 tgcttttaca gatgcacata tcgaggtgga catcacttac gctgagtact tcgaaatgtc 1020
1021 cgttcggttg gcagaagcta tgaaacgata tgggctgaat acaaatcaca gaatcgtcgt 1080
1081 atgcagtgaa aactctcttc aattctttat gccggtgttg ggcgcgttat ttatcggagt 1140
1141 tgcagttgcg cccgcgaacg acatttataa tgaacgtgaa ttgctcaaca gtatgggcat 1200
1201 ttcgcagcct accgtggtgt tcgtttccaa aaaggggttg caaaaaattt tgaacgtgca 1260
1261 aaaaaagctc ccaatcatcc aaaaaattat tatcatggat tctaaaacgg attaccaggg 1320
1321 atttcagtcg atgtacacgt tcgtcacatc tcatctacct cccggtttta atgaatacga 1380
1381 ttttgtgcca gagtccttcg atagggacaa gacaattgca ctgatcatga actcctctgg 1440
1441 atctactggt ctgcctaaag gtgtcgctct gcctcataga actgcctgcg tgagattctc 1500
1501 gcatgccaga gatcctattt ttggcaatca aatcattccg gatactgcga ttttaagtgt 1560
1561 tgttccattc catcacggtt ttggaatgtt tactacactc ggatatttga tatgtggatt 1620
1621 tcgagtcgtc ttaatgtata gatttgaaga agagctgttt ctgaggagcc ttcaggatta 1680
1681 caagattcaa agtgcgctgc tggtgccaac cctattctcc ttcttcgcca aaagcactct 1740
1741 gattgacaaa tacgatttat ctaatttaca cgaaattgct tctggtggcg ctcccctctc 1800
1801 taaggaagtc ggggaagcgg ttgccaagag gttccatctg ccaggtatca ggcaaggata 1860
1861 tgggctcact gagactacat cagctattct gattacaccc gagggggatg ataaaccggg 1920
1921 cgcggtcggt aaagttgttc cattttttga agcgaaggtt gtggatctgg ataccgggaa 1980
1981 aacgctgggc gttaatcaaa gaggcgaact gtgtgtgaga ggtcctatga ttatgtccgg 2040
2041 ttatgtaaac aatccggaag cgaccaacgc cttgattgac aaggatggat ggctacattc 2100
2101 tggagacata gcttactggg acgaagacga acacttcttc atcgttgacc gcctgaagtc 2160
2161 tctgattaag tacaaaggct atcaggtggc tcccgctgaa ttggaatcca tcttgctcca 2220
2221 acaccccaac atcttcgacg caggtgtcgc aggtcttccc gacgatgacg ccggtgaact 2280
2281 tcccgccgcc gttgttgttt tggagcacgg aaagacgatg acggaaaaag agatcgtgga 2340
2341 ttacgtcgcc agtcaagtaa caaccgcgaa aaagttgcgc ggaggagttg tgtttgtgga 2400
2401 cgaagtaccg aaaggtctta ccggaaaact cgacgcaaga aaaatcagag agatcctcat 2460
2461 aaaggccaag aagggcggaa agatcgccgt gtaattctag agggcccgtt taaaccgctg 2520
2521 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gttgcccggg cgcgatcgct 2580
2581 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat 2640
2641 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg 2700
2701 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg 2760
2761 tgggctctat ggcttctgag gcggaaagaa ccagcagatc gatctgcatc tatgtcgggt 2820
2821 gcggagaaag aggtaatgaa atggcattat gggtattatg ggtctgcatt aatgaatcgg 2880
2881 ccaacggatc ccggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc 2940
2941 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg 3000
3001 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa 3060
3061 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg 3120
3121 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga 3180
3181 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg 3240
3241 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg 3300
3301 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc 3360
3361 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg 3420
3421 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca 3480
3481 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt 3540
3541 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag 3600
3601 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg 3660
3661 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc 3720
3721 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt 3780
3781 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt 3840
3841 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca 3900
3901 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg 3960
3961 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac 4020
4021 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg 4080
4081 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc 4140
4141 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gaaaaaggat 4200
4201 cttcacctag atccttttca cgtagaaagc cagtccgcag aaacggtgct gaccccggat 4260
4261 gaatgtcagc tactgggcta tctggacaag ggaaaacgca agcgcaaaga gaaagcaggt 4320
4321 agcttgcagt gggcttacat ggcgatagct agactgggcg gttttatgga cagcaagcga 4380
4381 accggaattg ccagctgggg cgccctctgg taaggttggg aagccctgca aagtaaactg 4440
4441 gatggctttc tcgccgccaa ggatctgatg gcgcagggga tcaagctctg atcaagagac 4500
4501 aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc 4560
4561 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc 4620
4621 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc 4680
4681 cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg ccacgacggg 4740
4741 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt 4800
4801 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc 4860
4861 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga 4920
4921 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga 4980
4981 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct 5040
5041 caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc 5100
5101 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt 5160
5161 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg 5220
5221 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat 5280
5281 cgccttctat cgccttcttg acgagttctt ctgaattttg ttaaaatttt tgttaaatca 5340
5341 gctcattttt taaccaatag gccgaaatcg gcaacatccc ttataaatca aaagaataga 5400
5401 ccgcgatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg 5460
5461 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat 5520
5521 cacccaaatc aagttttttg cggtcgaggt gccgtaaagc tctaaatcgg aaccctaaag 5580
5581 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga 5640
5641 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa 5700
5701 ccaccacacc cgcgcgctta atgcgccgct acagggcgcg tccattcgcc attcaggatc 5760
5761 gaattaattc ttaattaaca tcatcaataa tatacctt 5798
LOCUS dna 5798 bp
FEATURES Location/Qualifiers
Other Gene 162..310
/gene="Encap other"
Promoter 365..393
/gene="amp prom"
misc_binding 457..462
/dbxref="REBASE:HpaI"
misc_binding 491..496
/dbxref="REBASE:BglII"
misc_binding 517..522
/dbxref="REBASE:AccI"
misc_binding 672..677
/dbxref="REBASE:KpnI"
misc_binding 678..683
/dbxref="REBASE:SacI"
misc_binding 712..717
/dbxref="REBASE:PstI"
misc_binding 717..722
/dbxref="REBASE:EcoRV"
misc_binding 733..740
/dbxref="REBASE:NotI"
misc_binding 740..745
/dbxref="REBASE:XhoI"
Reporter 842..2494
/gene="fLuc reporter"
ORF 842..2494
/sequence="ORF_1 rf(2)"
misc_binding 2502..2507
/dbxref="REBASE:ApaI"
misc_binding 2565..2570
/dbxref="REBASE:XmaI"
misc_binding 2565..2570
/dbxref="REBASE:SmaI"
misc_binding 2798..2803
/dbxref="REBASE:ClaI"
Rep_Origin 3110..3729
/gene="pBR322 origin"
misc_binding 4071..4081
/dbxref="REBASE:BglI"
selectable_marker249..5311
/gene="Kan"
Promoter 4382..4431
/gene="NEOKAN prom"
ORF 4520..5314
/sequence="ORF_2 rf(2)"
Marker 4523..5311
/gene="NTP_II marker"
Rep_Origin 5419..5724
/gene="f1 origin"
BASE COUNT 1461 a 1365 c 1569 g 1403 t 0 others
ORIGIN
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt
61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa
121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg
181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt
241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg
301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag
361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta
481 gcgaaagctc agatctggat ctcccgatcc cctatggtcg actctcagta caatctgctc
541 tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta
601 gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcttaagatt
661 taaataagct tggtaccgag ctcggatcca ctagtccagt gtggtggaat tctgcagata
721 tccagcacag tggcggccgc tcgagttcta gagggtatat aatggaagct cgaattccag
781 cttggcattc cggtactgtt ggtaaaaagc ttggcattcc ggtactgttg gtaaagccac
841 catggaagac gccaaaaaca taaagaaagg cccggcgcca ttctatccgc tggaagatgg
901 aaccgctgga gagcaactgc ataaggctat gaagagatac gccctggttc ctggaacaat
961 tgcttttaca gatgcacata tcgaggtgga catcacttac gctgagtact tcgaaatgtc
1021 cgttcggttg gcagaagcta tgaaacgata tgggctgaat acaaatcaca gaatcgtcgt
1081 atgcagtgaa aactctcttc aattctttat gccggtgttg ggcgcgttat ttatcggagt
1141 tgcagttgcg cccgcgaacg acatttataa tgaacgtgaa ttgctcaaca gtatgggcat
1201 ttcgcagcct accgtggtgt tcgtttccaa aaaggggttg caaaaaattt tgaacgtgca
1261 aaaaaagctc ccaatcatcc aaaaaattat tatcatggat tctaaaacgg attaccaggg
1321 atttcagtcg atgtacacgt tcgtcacatc tcatctacct cccggtttta atgaatacga
1381 ttttgtgcca gagtccttcg atagggacaa gacaattgca ctgatcatga actcctctgg
1441 atctactggt ctgcctaaag gtgtcgctct gcctcataga actgcctgcg tgagattctc
1501 gcatgccaga gatcctattt ttggcaatca aatcattccg gatactgcga ttttaagtgt
1561 tgttccattc catcacggtt ttggaatgtt tactacactc ggatatttga tatgtggatt
1621 tcgagtcgtc ttaatgtata gatttgaaga agagctgttt ctgaggagcc ttcaggatta
1681 caagattcaa agtgcgctgc tggtgccaac cctattctcc ttcttcgcca aaagcactct
1741 gattgacaaa tacgatttat ctaatttaca cgaaattgct tctggtggcg ctcccctctc
1801 taaggaagtc ggggaagcgg ttgccaagag gttccatctg ccaggtatca ggcaaggata
1861 tgggctcact gagactacat cagctattct gattacaccc gagggggatg ataaaccggg
1921 cgcggtcggt aaagttgttc cattttttga agcgaaggtt gtggatctgg ataccgggaa
1981 aacgctgggc gttaatcaaa gaggcgaact gtgtgtgaga ggtcctatga ttatgtccgg
2041 ttatgtaaac aatccggaag cgaccaacgc cttgattgac aaggatggat ggctacattc
2101 tggagacata gcttactggg acgaagacga acacttcttc atcgttgacc gcctgaagtc
2161 tctgattaag tacaaaggct atcaggtggc tcccgctgaa ttggaatcca tcttgctcca
2221 acaccccaac atcttcgacg caggtgtcgc aggtcttccc gacgatgacg ccggtgaact
2281 tcccgccgcc gttgttgttt tggagcacgg aaagacgatg acggaaaaag agatcgtgga
2341 ttacgtcgcc agtcaagtaa caaccgcgaa aaagttgcgc ggaggagttg tgtttgtgga
2401 cgaagtaccg aaaggtctta ccggaaaact cgacgcaaga aaaatcagag agatcctcat
2461 aaaggccaag aagggcggaa agatcgccgt gtaattctag agggcccgtt taaaccgctg
2521 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gttgcccggg cgcgatcgct
2581 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
2641 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
2701 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
2761 tgggctctat ggcttctgag gcggaaagaa ccagcagatc gatctgcatc tatgtcgggt
2821 gcggagaaag aggtaatgaa atggcattat gggtattatg ggtctgcatt aatgaatcgg
2881 ccaacggatc ccggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
2941 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
3001 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
3061 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
3121 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
3181 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
3241 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
3301 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
3361 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
3421 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
3481 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
3541 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
3601 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
3661 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
3721 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
3781 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
3841 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
3901 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
3961 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
4021 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
4081 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
4141 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gaaaaaggat
4201 cttcacctag atccttttca cgtagaaagc cagtccgcag aaacggtgct gaccccggat
4261 gaatgtcagc tactgggcta tctggacaag ggaaaacgca agcgcaaaga gaaagcaggt
4321 agcttgcagt gggcttacat ggcgatagct agactgggcg gttttatgga cagcaagcga
4381 accggaattg ccagctgggg cgccctctgg taaggttggg aagccctgca aagtaaactg
4441 gatggctttc tcgccgccaa ggatctgatg gcgcagggga tcaagctctg atcaagagac
4501 aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc
4561 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
4621 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
4681 cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg ccacgacggg
4741 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
4801 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
4861 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
4921 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
4981 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
5041 caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc
5101 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
5161 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
5221 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
5281 cgccttctat cgccttcttg acgagttctt ctgaattttg ttaaaatttt tgttaaatca
5341 gctcattttt taaccaatag gccgaaatcg gcaacatccc ttataaatca aaagaataga
5401 ccgcgatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
5461 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
5521 cacccaaatc aagttttttg cggtcgaggt gccgtaaagc tctaaatcgg aaccctaaag
5581 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
5641 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
5701 ccaccacacc cgcgcgctta atgcgccgct acagggcgcg tccattcgcc attcaggatc
5761 gaattaattc ttaattaaca tcatcaataa tatacctt
//
***Note: The text map shown below is representative of the parental
vector only and does not include the gene of interest. The sequence of the gene
can be found through the accession number link on the product page.
1 ttttggattgaagccaatatgataatgagggggtggagtttgtgacgtggcgcggggcgt 60
AAAACCTAACTTCGGTTATACTATTACTCCCCCACCTCAAACACTGCACCGCGCCCCGCA
61 gggaacggggcgggtgacgtagtagtgtggcggaagtgtgatgttgcaagtgtggcggaa 120
CCCTTGCCCCGCCCACTGCATCATCACACCGCCTTCACACTACAACGTTCACACCGCCTT
Encap other(162,310)>>>
|
121 cacatgtaagcgacggatgtggcaaaagtgacgtttttggtgtgcgccggtgtacacagg 180
GTGTACATTCGCTGCCTACACCGTTTTCACTGCAAAAACCACACGCGGCCACATGTGTCC
181 aagtgacaattttcgcgcggttttaggcggatgttgtagtaaatttgggcgtaaccgagt 240
TTCACTGTTAAAAGCGCGCCAAAATCCGCCTACAACATCATTTAAACCCGCATTGGCTCA
241 aagatttggccattttcgcgggaaaactgaataagaggaagtgaaatctgaataattttg 300
TTCTAAACCGGTAAAAGCGCCCTTTTGACTTATTCTCCTTCACTTTAGACTTATTAAAAC
301 tgttactcatagcgcgtaatacggcagacctcagcgctagattattgaagcatttatcag 360
ACAATGAGTATCGCGCATTATGCCGTCTGGAGTCGCGATCTAATAACTTCGTAAATAGTC
amp prom(365,393)<<<
|
361 ggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggg 420
CCAATAACAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTATCCC
HpaI
|
421 gttccgcgcacatttccccgaaaagtgccacctgacgttaactataacggtcctaaggta 480
CAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTGCAATTGATATTGCCAGGATTCCAT
BglII AccI
| |
481 gcgaaagctcagatctggatctcccgatcccctatggtcgactctcagtacaatctgctc 540
CGCTTTCGAGTCTAGACCTAGAGGGCTAGGGGATACCAGCTGAGAGTCATGTTAGACGAG
541 tgatgccgcatagttaagccagtatctgctccctgcttgtgtgttggaggtcgctgagta 600
ACTACGGCGTATCAATTCGGTCATAGACGAGGGACGAACACACAACCTCCAGCGACTCAT
601 gtgcgcgagcaaaatttaagctacaacaaggcaaggcttgaccgacaattgcttaagatt 660
CACGCGCTCGTTTTAAATTCGATGTTGTTCCGTTCCGAACTGGCTGTTAACGAATTCTAA
KpnI SacI PstI
| | |
661 taaataagcttggtaccgagctcggatccactagtccagtgtggtggaattctgcagata 720
ATTTATTCGAACCATGGCTCGAGCCTAGGTGATCAGGTCACACCACCTTAAGACGTCTAT
EcoRV NotI XhoI
| | |
721 tccagcacagtggcggccgctcgagttctagagggtatataatggaagctcgaattccag 780
AGGTCGTGTCACCGCCGGCGAGCTCAAGATCTCCCATATATTACCTTCGAGCTTAAGGTC
781 cttggcattccggtactgttggtaaaaagcttggcattccggtactgttggtaaagccac 840
GAACCGTAAGGCCATGACAACCATTTTTCGAACCGTAAGGCCATGACAACCATTTCGGTG
ORF_1 rf(2)(842,2494)>>>
|
fLuc reporter(842,2494)>>>
|
841 catggaagacgccaaaaacataaagaaaggcccggcgccattctatccgctggaagatgg 900
GTACCTTCTGCGGTTTTTGTATTTCTTTCCGGGCCGCGGTAAGATAGGCGACCTTCTACC
901 aaccgctggagagcaactgcataaggctatgaagagatacgccctggttcctggaacaat 960
TTGGCGACCTCTCGTTGACGTATTCCGATACTTCTCTATGCGGGACCAAGGACCTTGTTA
961 tgcttttacagatgcacatatcgaggtggacatcacttacgctgagtacttcgaaatgtc 1020
ACGAAAATGTCTACGTGTATAGCTCCACCTGTAGTGAATGCGACTCATGAAGCTTTACAG
1021 cgttcggttggcagaagctatgaaacgatatgggctgaatacaaatcacagaatcgtcgt 1080
GCAAGCCAACCGTCTTCGATACTTTGCTATACCCGACTTATGTTTAGTGTCTTAGCAGCA
1081 atgcagtgaaaactctcttcaattctttatgccggtgttgggcgcgttatttatcggagt 1140
TACGTCACTTTTGAGAGAAGTTAAGAAATACGGCCACAACCCGCGCAATAAATAGCCTCA
1141 tgcagttgcgcccgcgaacgacatttataatgaacgtgaattgctcaacagtatgggcat 1200
ACGTCAACGCGGGCGCTTGCTGTAAATATTACTTGCACTTAACGAGTTGTCATACCCGTA
1201 ttcgcagcctaccgtggtgttcgtttccaaaaaggggttgcaaaaaattttgaacgtgca 1260
AAGCGTCGGATGGCACCACAAGCAAAGGTTTTTCCCCAACGTTTTTTAAAACTTGCACGT
1261 aaaaaagctcccaatcatccaaaaaattattatcatggattctaaaacggattaccaggg 1320
TTTTTTCGAGGGTTAGTAGGTTTTTTAATAATAGTACCTAAGATTTTGCCTAATGGTCCC
1321 atttcagtcgatgtacacgttcgtcacatctcatctacctcccggttttaatgaatacga 1380
TAAAGTCAGCTACATGTGCAAGCAGTGTAGAGTAGATGGAGGGCCAAAATTACTTATGCT
1381 ttttgtgccagagtccttcgatagggacaagacaattgcactgatcatgaactcctctgg 1440
AAAACACGGTCTCAGGAAGCTATCCCTGTTCTGTTAACGTGACTAGTACTTGAGGAGACC
1441 atctactggtctgcctaaaggtgtcgctctgcctcatagaactgcctgcgtgagattctc 1500
TAGATGACCAGACGGATTTCCACAGCGAGACGGAGTATCTTGACGGACGCACTCTAAGAG
1501 gcatgccagagatcctatttttggcaatcaaatcattccggatactgcgattttaagtgt 1560
CGTACGGTCTCTAGGATAAAAACCGTTAGTTTAGTAAGGCCTATGACGCTAAAATTCACA
1561 tgttccattccatcacggttttggaatgtttactacactcggatatttgatatgtggatt 1620
ACAAGGTAAGGTAGTGCCAAAACCTTACAAATGATGTGAGCCTATAAACTATACACCTAA
1621 tcgagtcgtcttaatgtatagatttgaagaagagctgtttctgaggagccttcaggatta 1680
AGCTCAGCAGAATTACATATCTAAACTTCTTCTCGACAAAGACTCCTCGGAAGTCCTAAT
1681 caagattcaaagtgcgctgctggtgccaaccctattctccttcttcgccaaaagcactct 1740
GTTCTAAGTTTCACGCGACGACCACGGTTGGGATAAGAGGAAGAAGCGGTTTTCGTGAGA
1741 gattgacaaatacgatttatctaatttacacgaaattgcttctggtggcgctcccctctc 1800
CTAACTGTTTATGCTAAATAGATTAAATGTGCTTTAACGAAGACCACCGCGAGGGGAGAG
1801 taaggaagtcggggaagcggttgccaagaggttccatctgccaggtatcaggcaaggata 1860
ATTCCTTCAGCCCCTTCGCCAACGGTTCTCCAAGGTAGACGGTCCATAGTCCGTTCCTAT
1861 tgggctcactgagactacatcagctattctgattacacccgagggggatgataaaccggg 1920
ACCCGAGTGACTCTGATGTAGTCGATAAGACTAATGTGGGCTCCCCCTACTATTTGGCCC
1921 cgcggtcggtaaagttgttccattttttgaagcgaaggttgtggatctggataccgggaa 1980
GCGCCAGCCATTTCAACAAGGTAAAAAACTTCGCTTCCAACACCTAGACCTATGGCCCTT
1981 aacgctgggcgttaatcaaagaggcgaactgtgtgtgagaggtcctatgattatgtccgg 2040
TTGCGACCCGCAATTAGTTTCTCCGCTTGACACACACTCTCCAGGATACTAATACAGGCC
2041 ttatgtaaacaatccggaagcgaccaacgccttgattgacaaggatggatggctacattc 2100
AATACATTTGTTAGGCCTTCGCTGGTTGCGGAACTAACTGTTCCTACCTACCGATGTAAG
2101 tggagacatagcttactgggacgaagacgaacacttcttcatcgttgaccgcctgaagtc 2160
ACCTCTGTATCGAATGACCCTGCTTCTGCTTGTGAAGAAGTAGCAACTGGCGGACTTCAG
2161 tctgattaagtacaaaggctatcaggtggctcccgctgaattggaatccatcttgctcca 2220
AGACTAATTCATGTTTCCGATAGTCCACCGAGGGCGACTTAACCTTAGGTAGAACGAGGT
2221 acaccccaacatcttcgacgcaggtgtcgcaggtcttcccgacgatgacgccggtgaact 2280
TGTGGGGTTGTAGAAGCTGCGTCCACAGCGTCCAGAAGGGCTGCTACTGCGGCCACTTGA
2281 tcccgccgccgttgttgttttggagcacggaaagacgatgacggaaaaagagatcgtgga 2340
AGGGCGGCGGCAACAACAAAACCTCGTGCCTTTCTGCTACTGCCTTTTTCTCTAGCACCT
2341 ttacgtcgccagtcaagtaacaaccgcgaaaaagttgcgcggaggagttgtgtttgtgga 2400
AATGCAGCGGTCAGTTCATTGTTGGCGCTTTTTCAACGCGCCTCCTCAACACAAACACCT
2401 cgaagtaccgaaaggtcttaccggaaaactcgacgcaagaaaaatcagagagatcctcat 2460
GCTTCATGGCTTTCCAGAATGGCCTTTTGAGCTGCGTTCTTTTTAGTCTCTCTAGGAGTA
ApaI
|
2461 aaaggccaagaagggcggaaagatcgccgtgtaattctagagggcccgtttaaaccgctg 2520
TTTCCGGTTCTTCCCGCCTTTCTAGCGGCACATTAAGATCTCCCGGGCAAATTTGGCGAC
SmaI
|
XmaI
| |
2521 atcagcctcgactgtgccttctagttgccagccatctgttgttgcccgggcgcgatcgct 2580
TAGTCGGAGCTGACACGGAAGATCAACGGTCGGTAGACAACAACGGGCCCGCGCTAGCGA
2581 gcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaat 2640
CGGGGAGGGGGCACGGAAGGAACTGGGACCTTCCACGGTGAGGGTGACAGGAAAGGATTA
2641 aaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtgggg 2700
TTTTACTCCTTTAACGTAGCGTAACAGACTCATCCACAGTAAGATAAGACCCCCCACCCC
2701 tggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggatgcgg 2760
ACCCCGTCCTGTCGTTCCCCCTCCTAACCCTTCTGTTATCGTCCGTACGACCCCTACGCC
ClaI
|
2761 tgggctctatggcttctgaggcggaaagaaccagcagatcgatctgcatctatgtcgggt 2820
ACCCGAGATACCGAAGACTCCGCCTTTCTTGGTCGTCTAGCTAGACGTAGATACAGCCCA
2821 gcggagaaagaggtaatgaaatggcattatgggtattatgggtctgcattaatgaatcgg 2880
CGCCTCTTTCTCCATTACTTTACCGTAATACCCATAATACCCAGACGTAATTACTTAGCC
2881 ccaacggatcccggtgtgaaataccgcacagatgcgtaaggagaaaataccgcatcaggc 2940
GGTTGCCTAGGGCCACACTTTATGGCGTGTCTACGCATTCCTCTTTTATGGCGTAGTCCG
2941 gctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcgg 3000
CGAGAAGGCGAAGGAGCGAGTGACTGAGCGACGCGAGCCAGCAAGCCGACGCCGCTCGCC
3001 tatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaa 3060
ATAGTCGAGTGAGTTTCCGCCATTATGCCAATAGGTGTCTTAGTCCCCTATTGCGTCCTT
pBR322 origin(3110,3729)<<<
|
3061 agaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctgg 3120
TCTTGTACACTCGTTTTCCGGTCGTTTTCCGGTCCTTGGCATTTTTCCGGCGCAACGACC
3121 cgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcaga 3180
GCAAAAAGGTATCCGAGGCGGGGGGACTGCTCGTAGTGTTTTTAGCTGCGAGTTCAGTCT
3181 ggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcg 3240
CCACCGCTTTGGGCTGTCCTGATATTTCTATGGTCCGCAAAGGGGGACCTTCGAGGGAGC
3241 tgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgg 3300
ACGCGAGAGGACAAGGCTGGGACGGCGAATGGCCTATGGACAGGCGGAAAGAGGGAAGCC
3301 gaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttc 3360
CTTCGCACCGCGAAAGAGTATCGAGTGCGACATCCATAGAGTCAAGCCACATCCAGCAAG
3361 gctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccg 3420
CGAGGTTCGACCCGACACACGTGCTTGGGGGGCAAGTCGGGCTGGCGACGCGGAATAGGC
3421 gtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagcca 3480
CATTGATAGCAGAACTCAGGTTGGGCCATTCTGTGCTGAATAGCGGTGACCGTCGTCGGT
3481 ctggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggt 3540
GACCATTGTCCTAATCGTCTCGCTCCATACATCCGCCACGATGTCTCAAGAACTTCACCA
3541 ggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccag 3600
CCGGATTGATGCCGATGTGATCTTCCTGTCATAAACCATAGACGCGAGACGACTTCGGTC
3601 ttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcg 3660
AATGGAAGCCTTTTTCTCAACCATCGAGAACTAGGCCGTTTGTTTGGTGGCGACCATCGC
3661 gtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatc 3720
CACCAAAAAAACAAACGTTCGTCGTCTAATGCGCGTCTTTTTTTCCTAGAGTTCTTCTAG
3721 ctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattt 3780
GAAACTAGAAAAGATGCCCCAGACTGCGAGTCACCTTGCTTTTGAGTGCAATTCCCTAAA
3781 tggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagtt 3840
ACCAGTACTCTAATAGTTTTTCCTAGAAGTGGATCTAGGAAAATTTAATTTTTACTTCAA
3841 ttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatca 3900
AATTTAGTTAGATTTCATATATACTCATTTGAACCAGACTGTCAATGGTTACGAATTAGT
3901 gtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccg 3960
CACTCCGTGGATAGAGTCGCTAGACAGATAAAGCAAGTAGGTATCAACGGACTGAGGGGC
3961 tcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgatac 4020
AGCACATCTATTGATGCTATGCCCTCCCGAATGGTAGACCGGGGTCACGACGTTACTATG
BglI
|
4021 cgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaaggg 4080
GCGCTCTGGGTGCGAGTGGCCGAGGTCTAAATAGTCGTTATTTGGTCGGTCGGCCTTCCC
4081 ccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgcc 4140
GGCTCGCGTCTTCACCAGGACGTTGAAATAGGCGGAGGTAGGTCAGATAATTAACAACGG
4141 gggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgaaaaaggat 4200
CCCTTCGATCTCATTCATCAAGCGGTCAATTATCAAACGCGTTGCAACAACTTTTTCCTA
Kan(4249,5311)>>>
|
4201 cttcacctagatccttttcacgtagaaagccagtccgcagaaacggtgctgaccccggat 4260
GAAGTGGATCTAGGAAAAGTGCATCTTTCGGTCAGGCGTCTTTGCCACGACTGGGGCCTA
4261 gaatgtcagctactgggctatctggacaagggaaaacgcaagcgcaaagagaaagcaggt 4320
CTTACAGTCGATGACCCGATAGACCTGTTCCCTTTTGCGTTCGCGTTTCTCTTTCGTCCA
4321 agcttgcagtgggcttacatggcgatagctagactgggcggttttatggacagcaagcga 4380
TCGAACGTCACCCGAATGTACCGCTATCGATCTGACCCGCCAAAATACCTGTCGTTCGCT
NEOKAN prom(4382,4431)>>>
|
4381 accggaattgccagctggggcgccctctggtaaggttgggaagccctgcaaagtaaactg 4440
TGGCCTTAACGGTCGACCCCGCGGGAGACCATTCCAACCCTTCGGGACGTTTCATTTGAC
4441 gatggctttctcgccgccaaggatctgatggcgcaggggatcaagctctgatcaagagac 4500
CTACCGAAAGAGCGGCGGTTCCTAGACTACCGCGTCCCCTAGTTCGAGACTAGTTCTCTG
NTP_II marker(4523,5311)>>>
|
ORF_2 rf(2)(4520,5314)>>>
| |
4501 aggatgaggatcgtttcgcatgattgaacaagatggattgcacgcaggttctccggccgc 4560
TCCTACTCCTAGCAAAGCGTACTAACTTGTTCTACCTAACGTGCGTCCAAGAGGCCGGCG
4561 ttgggtggagaggctattcggctatgactgggcacaacagacaatcggctgctctgatgc 4620
AACCCACCTCTCCGATAAGCCGATACTGACCCGTGTTGTCTGTTAGCCGACGAGACTACG
4621 cgccgtgttccggctgtcagcgcaggggcgcccggttctttttgtcaagaccgacctgtc 4680
GCGGCACAAGGCCGACAGTCGCGTCCCCGCGGGCCAAGAAAAACAGTTCTGGCTGGACAG
4681 cggtgccctgaatgaactgcaagacgaggcagcgcggctatcgtggctggccacgacggg 4740
GCCACGGGACTTACTTGACGTTCTGCTCCGTCGCGCCGATAGCACCGACCGGTGCTGCCC
4741 cgttccttgcgcagctgtgctcgacgttgtcactgaagcgggaagggactggctgctatt 4800
GCAAGGAACGCGTCGACACGAGCTGCAACAGTGACTTCGCCCTTCCCTGACCGACGATAA
4801 gggcgaagtgccggggcaggatctcctgtcatctcaccttgctcctgccgagaaagtatc 4860
CCCGCTTCACGGCCCCGTCCTAGAGGACAGTAGAGTGGAACGAGGACGGCTCTTTCATAG
4861 catcatggctgatgcaatgcggcggctgcatacgcttgatccggctacctgcccattcga 4920
GTAGTACCGACTACGTTACGCCGCCGACGTATGCGAACTAGGCCGATGGACGGGTAAGCT
4921 ccaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaagccggtcttgtcga 4980
GGTGGTTCGCTTTGTAGCGTAGCTCGCTCGTGCATGAGCCTACCTTCGGCCAGAACAGCT
4981 tcaggatgatctggacgaagagcatcaggggctcgcgccagccgaactgttcgccaggct 5040
AGTCCTACTAGACCTGCTTCTCGTAGTCCCCGAGCGCGGTCGGCTTGACAAGCGGTCCGA
5041 caaggcgagcatgcccgacggcgaggatctcgtcgtgacccatggcgatgcctgcttgcc 5100
GTTCCGCTCGTACGGGCTGCCGCTCCTAGAGCAGCACTGGGTACCGCTACGGACGAACGG
5101 gaatatcatggtggaaaatggccgcttttctggattcatcgactgtggccggctgggtgt 5160
CTTATAGTACCACCTTTTACCGGCGAAAAGACCTAAGTAGCTGACACCGGCCGACCCACA
5161 ggcggaccgctatcaggacatagcgttggctacccgtgatattgctgaagagcttggcgg 5220
CCGCCTGGCGATAGTCCTGTATCGCAACCGATGGGCACTATAACGACTTCTCGAACCGCC
5221 cgaatgggctgaccgcttcctcgtgctttacggtatcgccgctcccgattcgcagcgcat 5280
GCTTACCCGACTGGCGAAGGAGCACGAAATGCCATAGCGGCGAGGGCTAAGCGTCGCGTA
5281 cgccttctatcgccttcttgacgagttcttctgaattttgttaaaatttttgttaaatca 5340
GCGGAAGATAGCGGAAGAACTGCTCAAGAAGACTTAAAACAATTTTAAAAACAATTTAGT
5341 gctcattttttaaccaataggccgaaatcggcaacatcccttataaatcaaaagaataga 5400
CGAGTAAAAAATTGGTTATCCGGCTTTAGCCGTTGTAGGGAATATTTAGTTTTCTTATCT
f1 origin(5419,5724)<<<
|
5401 ccgcgatagggttgagtgttgttccagtttggaacaagagtccactattaaagaacgtgg 5460
GGCGCTATCCCAACTCACAACAAGGTCAAACCTTGTTCTCAGGTGATAATTTCTTGCACC
5461 actccaacgtcaaagggcgaaaaaccgtctatcagggcgatggcccactacgtgaaccat 5520
TGAGGTTGCAGTTTCCCGCTTTTTGGCAGATAGTCCCGCTACCGGGTGATGCACTTGGTA
5521 cacccaaatcaagttttttgcggtcgaggtgccgtaaagctctaaatcggaaccctaaag 5580
GTGGGTTTAGTTCAAAAAACGCCAGCTCCACGGCATTTCGAGATTTAGCCTTGGGATTTC
5581 ggagcccccgatttagagcttgacggggaaagccggcgaacgtggcgagaaaggaaggga 5640
CCTCGGGGGCTAAATCTCGAACTGCCCCTTTCGGCCGCTTGCACCGCTCTTTCCTTCCCT
5641 agaaagcgaaaggagcgggcgctagggcgctggcaagtgtagcggtcacgctgcgcgtaa 5700
TCTTTCGCTTTCCTCGCCCGCGATCCCGCGACCGTTCACATCGCCAGTGCGACGCGCATT
5701 ccaccacacccgcgcgcttaatgcgccgctacagggcgcgtccattcgccattcaggatc 5760
GGTGGTGTGGGCGCGCGAATTACGCGGCGATGTCCCGCGCAGGTAAGCGGTAAGTCCTAG
5761 gaattaattcttaattaacatcatcaataatatacctt 5798
CTTAATTAAGAATTAATTGTAGTAGTTATTATATGGAA
Misc. Feature (5 total)
I-Ceu I
Start: 459 End: 484
Luciferase
Start: 748 End: 2497
PI-Sce I
Start: 2808 End: 2846
Ori
Start: 3001 End: 3780
Kan
Start: 4520 End: 5311