pLenti-promoter-GFP
| Vector Name | pLenti-promoter-GFP |
| VectorType | Lentivirus |
| Antibiotic Information | Puromycin |
| Additional Information | No Additional Information |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg 61 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg 121 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct 181 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt 241 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga 301 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg 361 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga 421 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa 481 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa 541 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag 601 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat 661 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga 721 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta 781 agaccaccgc acagcaagcc cgctgatctt cagacctgga ggaggagata tgagggacat 841 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc 901 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg 961 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg 1021 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct 1081 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca 1141 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc 1201 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct 1261 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac 1321 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa 1381 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg 1441 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt 1501 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag 1561 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga 1621 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcgaaagctt 1681 gggattcgaa tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 1741 agacataata gcaacagaca tacaaactaa agaactacaa aaacaaatta caaaaattca 1801 aaattttcgg gtttttcgaa cctagccccg ggatcgatca attgggggag ctcacgggga 1861 cagccccccc ccaaagcccc cagggatgta attacgtccc tcccccgcta gggggcagca 1921 gcgaccgccc ggggctccgc tccggtccgg cgctcccccc gcatcccgag ccggcagcgt 1981 gcggggacag cccgggcacg gggaaggtgg cacgggatcg ctttcctctg aacgcttctc 2041 gctgctcttt gagcctgcag acacctgggg gatacgggga aaaggtaccg gggagctcac 2101 ggggacagcc cccccccaaa gcccccaggg atgtaattac gtccctcccc gctagggggc 2161 agcagcgacc gcccggggct ccgctccggt ccggcgctcc ccccgcatcc cgagccggca 2221 gcgtgcgggg acagcccggg cacggggaag gtggcacggg atcgctttcc tctgaacgct 2281 tctcgctgct ctttgagcct gcagacacct gggggatacg gggaaaagaa ttgggggagc 2341 tcacggggac agcccccccc caaagccccc agggatgtaa ttacgtccct cccccgctag 2401 ggggcagcag cgaccgcccg gggctccgct ccggtccggc gctccccccc atcccgagcc 2461 ggcagcgtgc ggggacagcc cgggcacggg gaaggtggca cgggatcgct tcctctgaac 2521 gcttctcgct gctctttgag cctgcagaca cctgggggat acggggaaaa acactggggt 2581 acctacgtaa gggagctcac ggggacagcc cccccccaaa gcccccaggg atgtaattac 2641 gtccctcccc gctagggggc agcagcgacc gcccggggct ccgctccggt ccggcgctcc 2701 ccccgcatcc cgagccggca gcgtgcgggg acagcccggg cacggggaag gtggcacggg 2761 atcgctttcc tctgaacgct tctcgctgct ctttgagcct gcagacacct gggggatacg 2821 gggaaaagaa ttcactagta ccggtaggcc tgtcgacgat atcgggcccg cggccgctgg 2881 atcctctaga ctgcagctcg agatggtgag caagggcgag gagctgttca ccggggtggt 2941 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga 3001 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa 3061 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag 3121 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta 3181 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt 3241 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga 3301 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat 3361 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga 3421 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc 3481 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa 3541 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg 3601 catggacgag ctgtacaagg ggcaccacca ccaccaccac tgagtcgagt acccatacga 3661 cgtcccagac tacgcttgag tttaaacacg cgtggtgtgg aaagtcccca ggctccccag 3721 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc 3781 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag 3841 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc 3901 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc 3961 tattccagaa gtagtgagga ggcttttttg gaggccatga ccgagtacaa gcccacggtg 4021 cgcctcgcca cccgcgacga cgtccctcgg gccgtacgca ccctcgccgc cgcgttcgcc 4081 gactaccccg ccacgcgcca caccgtggac ccggaccgcc acatcgagcg ggtcaccgag 4141 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac 4201 gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc 4261 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag 4321 atggaagggc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc 4381 ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg 4441 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc 4501 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg 4561 cgcacctggt gcatgacccg caagcccggt gcctgaacgc gttccggaaa tcaacctctg 4621 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta 4681 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt 4741 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc 4801 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt 4861 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg 4921 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac 4981 aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc 5041 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac 5101 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtctcgc cttcgccctc 5161 agacgagtcg gatctccctt tgggccgcct ccccgcctgt ccggatggaa gggctaattc 5221 actcccaacg aatacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga 5281 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct 5341 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat 5401 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta 5461 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta 5521 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat 5581 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct 5641 ggcatctatg tcgggtgcgg agaaagaggt aatgaaatgg cattatgggt attatgggtc 5701 tgcattaatg aatcggccaa cggatgtcgg tgtgaaatac cgcacagatg cgtaaggaga 5761 aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt 5821 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca 5881 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa 5941 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat 6001 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc 6061 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc 6121 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt 6181 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac 6241 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg 6301 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca 6361 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc 6421 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa 6481 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa 6541 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac 6601 tcacgttaag ggattttggc catgagatta tcaaaaagga tcttcaccta gatccttttc 6661 acgtagaaag ccagtccgca gaaacggtgc tgaccccgga tgaatgtcag ctactgggct 6721 atctggacaa gggaaaacgc aagcgcaaag agaaagcagg tagcttgcag tgggcttaca 6781 tggcgatagc tagactgggc ggttttatgg acagcaagcg aaccggaatt gccagctggg 6841 gcgccctctg gtaaggttgg gaagccctgc aaagtaaact ggatggcttt cttgccgcca 6901 aggatctgat ggcgcagggg atcaagctct gatcaagaga caggatgagg atcgtttcgc 6961 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc 7021 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca 7081 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg 7141 caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg 7201 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag 7261 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg 7321 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc 7381 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa 7441 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag catgcccgac 7501 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat 7561 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac 7621 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc 7681 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt 7741 gacgagttct tctgaatttt gttaaaattt ttgttaaatc agctcatttt ttaaccaata 7801 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt 7861 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg 7921 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt 7981 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc 8041 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg 8101 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct 8161 taatgcgccg ctacagggcg cgtccattcg ccattcagga tcgaattaat tcttaattaa 8221 catcatcaat aatatacctt ttttggattg aagccaatat gataatgagg gggtggagtt 8281 tgtgacgtgg cgcggggcgt gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg 8341 atgttgcaag tgtggcggaa cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg 8401 tgtgcgccgg tgtacacagg aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt 8461 aaatttgggc gtaaccgagt aagatttggc cattttcgcg ggaaaactga ataagaggaa 8521 gtgaaatctg aataattttg tgttactcat agcgcgtaat actctcatga gcggatacat 8581 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt 8641 gccacctgac gtaactataa cggtcctaag gtagcgaaaa tgtagtctta tgcaatactc 8701 ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg agagaaaaag 8761 caccgtgcat gccgattggt ggaagtaagg tggtacgat
LOCUS dna 8799 bp FEATURES Location/Qualifiers Other Gene 109..289 /gene="HIV-1_5_LTR other" Other Gene 400..444 /gene="HIV-1_psi_pack other" misc_binding 486..491 /dbxref="REBASE:NruI" Regulatory_Seq 951..1184 /gene="RRE reg" ORF 994..1695 /sequence="ORF_1 rf(1)" misc_binding 1833..1838 /dbxref="REBASE:ClaI" ORF 2341..2946 /sequence="ORF_5 rf(4)" ORF 2597..3742 /sequence="ORF_6 rf(6)" misc_binding 2828..2833 /dbxref="REBASE:EcoRI" misc_binding 2846..2851 /dbxref="REBASE:StuI" misc_binding 2852..2857 /dbxref="REBASE:HincII" misc_binding 2858..2863 /dbxref="REBASE:EcoRV" misc_binding 2864..2869 /dbxref="REBASE:ApaI" misc_binding 2870..2877 /dbxref="REBASE:NotI" misc_binding 2879..2884 /dbxref="REBASE:BamHI" misc_binding 2885..2890 /dbxref="REBASE:XbaI" misc_binding 2897..2902 /dbxref="REBASE:XhoI" Reporter 2903..3619 /gene="EGFP reporter" ORF 2903..3643 /sequence="ORF_4 rf(2)" Promoter 3694..3962 /gene="SV40 prom" Rep_Origin 3861..3938 /gene="SV40 origin" ORF 3928..4596 /sequence="ORF_2 rf(1)" misc_binding 3941..3953 /dbxref="REBASE:SfiI" Marker 3997..4596 /gene="puro marker" Other Gene 5206..5258 /gene="delta_U3 other" Other Gene 5259..5439 /gene="HIV-1_5_LTR other" Rep_Origin 5946..6565 /gene="pBR322 origin" Promoter 6823..6872 /gene="NEOKAN prom" misc_binding 6930..6935 /dbxref="REBASE:BclI" ORF 6961..7755 /sequence="ORF_3 rf(1)" Marker 6964..7752 /gene="NTP_II marker" Rep_Origin 7860..8166 /gene="f1 origin" Other Gene 8221..8322 /gene="RITR other" Other Gene 8221..8322 /gene="LITR other" Other Gene 8402..8550 /gene="Encap other" BASE COUNT 2113 a 2345 c 2495 g 1846 t 0 others ORIGIN 1 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg 61 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg 121 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct 181 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt 241 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga 301 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg 361 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga 421 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa 481 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa 541 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag 601 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat 661 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga 721 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta 781 agaccaccgc acagcaagcc cgctgatctt cagacctgga ggaggagata tgagggacat 841 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc 901 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg 961 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg 1021 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct 1081 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca 1141 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc 1201 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct 1261 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac 1321 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa 1381 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg 1441 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt 1501 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag 1561 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga 1621 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcgaaagctt 1681 gggattcgaa tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 1741 agacataata gcaacagaca tacaaactaa agaactacaa aaacaaatta caaaaattca 1801 aaattttcgg gtttttcgaa cctagccccg ggatcgatca attgggggag ctcacgggga 1861 cagccccccc ccaaagcccc cagggatgta attacgtccc tcccccgcta gggggcagca 1921 gcgaccgccc ggggctccgc tccggtccgg cgctcccccc gcatcccgag ccggcagcgt 1981 gcggggacag cccgggcacg gggaaggtgg cacgggatcg ctttcctctg aacgcttctc 2041 gctgctcttt gagcctgcag acacctgggg gatacgggga aaaggtaccg gggagctcac 2101 ggggacagcc cccccccaaa gcccccaggg atgtaattac gtccctcccc gctagggggc 2161 agcagcgacc gcccggggct ccgctccggt ccggcgctcc ccccgcatcc cgagccggca 2221 gcgtgcgggg acagcccggg cacggggaag gtggcacggg atcgctttcc tctgaacgct 2281 tctcgctgct ctttgagcct gcagacacct gggggatacg gggaaaagaa ttgggggagc 2341 tcacggggac agcccccccc caaagccccc agggatgtaa ttacgtccct cccccgctag 2401 ggggcagcag cgaccgcccg gggctccgct ccggtccggc gctccccccc atcccgagcc 2461 ggcagcgtgc ggggacagcc cgggcacggg gaaggtggca cgggatcgct tcctctgaac 2521 gcttctcgct gctctttgag cctgcagaca cctgggggat acggggaaaa acactggggt 2581 acctacgtaa gggagctcac ggggacagcc cccccccaaa gcccccaggg atgtaattac 2641 gtccctcccc gctagggggc agcagcgacc gcccggggct ccgctccggt ccggcgctcc 2701 ccccgcatcc cgagccggca gcgtgcgggg acagcccggg cacggggaag gtggcacggg 2761 atcgctttcc tctgaacgct tctcgctgct ctttgagcct gcagacacct gggggatacg 2821 gggaaaagaa ttcactagta ccggtaggcc tgtcgacgat atcgggcccg cggccgctgg 2881 atcctctaga ctgcagctcg agatggtgag caagggcgag gagctgttca ccggggtggt 2941 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga 3001 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa 3061 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag 3121 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta 3181 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt 3241 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga 3301 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat 3361 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga 3421 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc 3481 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa 3541 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg 3601 catggacgag ctgtacaagg ggcaccacca ccaccaccac tgagtcgagt acccatacga 3661 cgtcccagac tacgcttgag tttaaacacg cgtggtgtgg aaagtcccca ggctccccag 3721 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc 3781 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag 3841 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc 3901 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc 3961 tattccagaa gtagtgagga ggcttttttg gaggccatga ccgagtacaa gcccacggtg 4021 cgcctcgcca cccgcgacga cgtccctcgg gccgtacgca ccctcgccgc cgcgttcgcc 4081 gactaccccg ccacgcgcca caccgtggac ccggaccgcc acatcgagcg ggtcaccgag 4141 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac 4201 gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc 4261 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag 4321 atggaagggc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc 4381 ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg 4441 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc 4501 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg 4561 cgcacctggt gcatgacccg caagcccggt gcctgaacgc gttccggaaa tcaacctctg 4621 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta 4681 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt 4741 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc 4801 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt 4861 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg 4921 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac 4981 aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc 5041 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac 5101 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtctcgc cttcgccctc 5161 agacgagtcg gatctccctt tgggccgcct ccccgcctgt ccggatggaa gggctaattc 5221 actcccaacg aatacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga 5281 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct 5341 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat 5401 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta 5461 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta 5521 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat 5581 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct 5641 ggcatctatg tcgggtgcgg agaaagaggt aatgaaatgg cattatgggt attatgggtc 5701 tgcattaatg aatcggccaa cggatgtcgg tgtgaaatac cgcacagatg cgtaaggaga 5761 aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt 5821 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca 5881 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa 5941 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat 6001 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc 6061 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc 6121 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt 6181 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac 6241 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg 6301 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca 6361 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc 6421 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa 6481 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa 6541 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac 6601 tcacgttaag ggattttggc catgagatta tcaaaaagga tcttcaccta gatccttttc 6661 acgtagaaag ccagtccgca gaaacggtgc tgaccccgga tgaatgtcag ctactgggct 6721 atctggacaa gggaaaacgc aagcgcaaag agaaagcagg tagcttgcag tgggcttaca 6781 tggcgatagc tagactgggc ggttttatgg acagcaagcg aaccggaatt gccagctggg 6841 gcgccctctg gtaaggttgg gaagccctgc aaagtaaact ggatggcttt cttgccgcca 6901 aggatctgat ggcgcagggg atcaagctct gatcaagaga caggatgagg atcgtttcgc 6961 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc 7021 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca 7081 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg 7141 caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg 7201 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag 7261 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg 7321 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc 7381 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa 7441 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag catgcccgac 7501 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat 7561 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac 7621 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc 7681 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt 7741 gacgagttct tctgaatttt gttaaaattt ttgttaaatc agctcatttt ttaaccaata 7801 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt 7861 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg 7921 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt 7981 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc 8041 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg 8101 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct 8161 taatgcgccg ctacagggcg cgtccattcg ccattcagga tcgaattaat tcttaattaa 8221 catcatcaat aatatacctt ttttggattg aagccaatat gataatgagg gggtggagtt 8281 tgtgacgtgg cgcggggcgt gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg 8341 atgttgcaag tgtggcggaa cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg 8401 tgtgcgccgg tgtacacagg aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt 8461 aaatttgggc gtaaccgagt aagatttggc cattttcgcg ggaaaactga ataagaggaa 8521 gtgaaatctg aataattttg tgttactcat agcgcgtaat actctcatga gcggatacat 8581 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt 8641 gccacctgac gtaactataa cggtcctaag gtagcgaaaa tgtagtctta tgcaatactc 8701 ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg agagaaaaag 8761 caccgtgcat gccgattggt ggaagtaagg tggtacgat //
LOCUS dna 8799 bp FEATURES Location/Qualifiers Other Gene 109..289 /gene="HIV-1_5_LTR other" Other Gene 400..444 /gene="HIV-1_psi_pack other" misc_binding 486..491 /dbxref="REBASE:NruI" Regulatory_Seq 951..1184 /gene="RRE reg" ORF 994..1695 /sequence="ORF_1 rf(1)" misc_binding 1833..1838 /dbxref="REBASE:ClaI" ORF 2341..2946 /sequence="ORF_5 rf(4)" ORF 2597..3742 /sequence="ORF_6 rf(6)" misc_binding 2828..2833 /dbxref="REBASE:EcoRI" misc_binding 2846..2851 /dbxref="REBASE:StuI" misc_binding 2852..2857 /dbxref="REBASE:HincII" misc_binding 2858..2863 /dbxref="REBASE:EcoRV" misc_binding 2864..2869 /dbxref="REBASE:ApaI" misc_binding 2870..2877 /dbxref="REBASE:NotI" misc_binding 2879..2884 /dbxref="REBASE:BamHI" misc_binding 2885..2890 /dbxref="REBASE:XbaI" misc_binding 2897..2902 /dbxref="REBASE:XhoI" Reporter 2903..3619 /gene="EGFP reporter" ORF 2903..3643 /sequence="ORF_4 rf(2)" Promoter 3694..3962 /gene="SV40 prom" Rep_Origin 3861..3938 /gene="SV40 origin" ORF 3928..4596 /sequence="ORF_2 rf(1)" misc_binding 3941..3953 /dbxref="REBASE:SfiI" Marker 3997..4596 /gene="puro marker" Other Gene 5206..5258 /gene="delta_U3 other" Other Gene 5259..5439 /gene="HIV-1_5_LTR other" Rep_Origin 5946..6565 /gene="pBR322 origin" Promoter 6823..6872 /gene="NEOKAN prom" misc_binding 6930..6935 /dbxref="REBASE:BclI" ORF 6961..7755 /sequence="ORF_3 rf(1)" Marker 6964..7752 /gene="NTP_II marker" Rep_Origin 7860..8166 /gene="f1 origin" Other Gene 8221..8322 /gene="RITR other" Other Gene 8221..8322 /gene="LITR other" Other Gene 8402..8550 /gene="Encap other" BASE COUNT 2113 a 2345 c 2495 g 1846 t 0 others