pLenti-hTERT-No-Selection
Vector Name | pLenti-hTERT-No-Selection |
VectorType | Lentiviral plasmid |
Antibiotic Information | No selection marker needed for cell immortalization. Primary cells that are not successfully immortalized will die after several passage rounds. |
Sequencing Primers | CMV sequencing primer 5`---CGC AAA TGG GCG GTA GGC GTG---3` |
Additional Information | The gene expression is driven by the CMV promoter, without any Tag or reporter. |
***Note: Features may not be to scale, please refer to sequence or text map for detailed information.
***Note: The vector sequence shown below is representative of the parental vector
only and does not include the gene of interest. The sequence of the gene can be found
through the accession number link on the product page. The restriction sites within the
cloning region may differ from the provided sequence.
1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt 61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa 121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg 181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt 241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg 301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag 361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta 481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag 541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg 601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact 661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct 721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag 1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta 1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga 1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg 1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg 1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc 1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg 1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat 1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac 1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat 1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca 2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga 2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg 2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa 2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga 2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag 2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa 2401 aattcaaaat tttcgggttt ttcgaaccta gggttccgcg ttacataact tacggtaaat 2461 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt 2521 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa 2581 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc 2641 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct 2701 acttggcagt acatctacgt ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 2761 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 2821 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 2881 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 2941 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc 3001 atagaagaac cgagtttaaa ctccctatca gtgatagaga tctccctatc agtgatagag 3061 agctagcccc gggatcgatc aattgagtac ttacgtaggt accccagtgt ggtggcctgc 3121 aggtgaattc caccatgccg cgcgctcccc gctgccgagc cgtgcgctcc ctgctgcgca 3181 gccactaccg cgaggtgctg ccgctggcca cgttcgtgcg gcgcctgggg ccccagggct 3241 ggcggctggt gcagcgcggg gacccggcgg ctttccgcgc gctggtggcc cagtgcctgg 3301 tgtgcgtgcc ctgggacgca cggccgcccc ccgccgcccc ctccttccgc caggtgtcct 3361 gcctgaagga gctggtggcc cgagtgctgc agaggctgtg cgagcgcggc gcgaagaacg 3421 tgctggcctt cggcttcgcg ctgctggacg gggcccgcgg gggccccccc gaggccttca 3481 ccaccagcgt gcgcagctac ctgcccaaca cggtgaccga cgcactgcgg gggagcgggg 3541 cgtgggggct gctgctgcgc cgcgtgggcg acgacgtgct ggttcacctg ctggcacgct 3601 gcgcgctctt tgtgctggtg gctcccagct gcgcctacca ggtgtgcggg ccgccgctgt 3661 accagctcgg cgctgccact caggcccggc ccccgccaca cgctagtgga ccccgaaggc 3721 gtctgggatg cgaacgggcc tggaaccata gcgtcaggga ggccggggtc cccctgggcc 3781 tgccagcccc gggtgcgagg aggcgcgggg gcagtgccag ccgaagtctg ccgttgccca 3841 agaggcccag gcgtggcgct gcccctgagc cggagcggac gcccgttggg caggggtcct 3901 gggcccaccc gggcaggacg cgtggaccga gtgaccgtgg tttctgtgtg gtgtcacctg 3961 ccagacccgc cgaagaagcc acctctttgg agggtgcgct ctctggcacg cgccactccc 4021 acccatccgt gggccgccag caccacgcgg gccccccatc cacatcgcgg ccaccacgtc 4081 cctgggacac gccttgtccc ccggtgtacg ccgagaccaa gcacttcctc tactcctcag 4141 gcgacaagga gcagctgcgg ccctccttcc tactcagctc tctgaggccc agcctgactg 4201 gcgctcggag gctcgtggag accatctttc tgggttccag gccctggatg ccagggactc 4261 cccgcaggtt gccccgcctg ccccagcgct actggcaaat gcggcccctg tttctggagc 4321 tgcttgggaa ccacgcgcag tgcccctacg gggtgctcct caagacgcac tgcccgctgc 4381 gagctgcggt caccccagca gccggtgtct gtgcccggga gaagccccag ggctctgtgg 4441 cggcccccga ggaggaggac acagaccccc gtcgcctggt gcagctgctc cgccagcaca 4501 gcagcccctg gcaggtgtac ggcttcgtgc gggcctgcct gcgccggctg gtgcccccag 4561 gcctctgggg ctccaggcac aacgaacgcc gcttcctcag gaacaccaag aagttcatct 4621 ccctggggaa gcatgccaag ctctcgctgc aggagctgac gtggaagatg agcgtgcggg 4681 actgcgcttg gctgcgcagg agcccagggg ttggctgtgt tccggccgca gagcaccgtc 4741 tgcgtgagga gatcctggcc aagttcctgc actggctgat gagtgtgtac gtcgtcgagc 4801 tgctcaggtc tttcttttat gtcacggaga ccacgtttca aaagaacagg ctctttttct 4861 accggaagag tgtctggagc aagttgcaaa gcattggaat cagacagcac ttgaagaggg 4921 tgcagctgcg ggagctgtcg gaagcagagg tcaggcagca tcgggaagcc aggcccgccc 4981 tgctgacgtc cagactccgc ttcatcccca agcctgacgg gctgcggccg attgtgaaca 5041 tggactacgt cgtgggagcc agaacgttcc gcagagaaaa gagggccgag cgtctcacct 5101 cgagggtgaa ggcactgttc agcgtgctca actacgagcg ggcgcggcgc cccggcctcc 5161 tgggcgcctc tgtgctgggc ctggacgata tccacagggc ctggcgcacc ttcgtgctgc 5221 gtgtgcgggc ccaggacccg ccgcctgagc tgtactttgt caaggtggat gtgacgggcg 5281 cgtacgacac catcccccag gacaggctca cggaggtcat cgccagcatc atcaaacccc 5341 agaacacgta ctgcgtgcgt cggtatgccg tggtccagaa ggccgcccat gggcacgtcc 5401 gcaaggcctt caagagccac gtctctacct tgacagacct ccagccgtac atgcgacagt 5461 tcgtggctca cctgcaggag accagcccgc tgagggatgc cgtcgtcatc gagcagagct 5521 cctccctgaa tgaggccagc agtggcctct tcgacgtctt cctacgcttc atgtgccacc 5581 acgccgtgcg catcaggggc aagtcctacg tccagtgcca ggggatcccg cagggctcca 5641 tcctctccac gctgctctgc agcctgtgct acggcgacat ggagaacaag ctgtttgcgg 5701 ggattcggcg ggacgggctg ctcctgcgtt tggtggatga tttcttgttg gtgacacctc 5761 acctcaccca cgcgaaaacc ttcctcagga ccctggtccg aggtgtccct gagtatggct 5821 gcgtggtgaa cttgcggaag acagtggtga acttccctgt agaagacgag gccctgggtg 5881 gcacggcttt tgttcagatg ccggcccacg gcctattccc ctggtgcggc ctgctgctgg 5941 atacccggac cctggaggtg cagagcgact actccagcta tgcccggacc tccatcagag 6001 ccagtctcac cttcaaccgc ggcttcaagg ctgggaggaa catgcgtcgc aaactctttg 6061 gggtcttgcg gctgaagtgt cacagcctgt ttctggattt gcaggtgaac agcctccaga 6121 cggtgtgcac caacatctac aagatcctcc tgctgcaggc gtacaggttt cacgcatgtg 6181 tgctgcagct cccatttcat cagcaagttt ggaagaaccc cacatttttc ctgcgcgtca 6241 tctctgacac ggcctccctc tgctactcca tcctgaaagc caagaacgca gggatgtcgc 6301 tgggggccaa gggcgccgcc ggccctctgc cctccgaggc cgtgcagtgg ctgtgccacc 6361 aagcattcct gctcaagctg actcgacacc gtgtcaccta cgtgccactc ctggggtcac 6421 tcaggacagc ccagacgcag ctgagtcgga agctcccggg gacgacgctg actgccctgg 6481 aggccgcagc caacccggca ctgccctcag acttcaagac catcctggac tgatgggacg 6541 cggccgcatg actagttccg gaaatcaacc tctggattac aaaatttgtg aaagattgac 6601 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt 6661 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt 6721 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt 6781 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg 6841 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg 6901 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct 6961 gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt 7021 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc 7081 tctgcggcct cttccgcgtc tcgccttcgc cctcagacga gtcggatctc cctttgggcc 7141 gcctccccgc ctgtccggat ggaagggcta attcactccc aacgaataca agatctgctt 7201 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa 7261 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt 7321 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg 7381 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag 7441 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa 7501 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt 7561 ttgtccaaac tcatcaatgt atcttatcat gtctggcatc tatgtcgggt gcggagaaag 7621 aggtaatgaa atggcattat gggtattatg ggtctgcatt aatgaatcgg ccaacgatcc 7681 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct 7741 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac 7801 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga 7861 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat 7921 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac 7981 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct 8041 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg 8101 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg 8161 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt 8221 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg 8281 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac 8341 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga 8401 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt 8461 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt 8521 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga 8581 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc 8641 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct 8701 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata 8761 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca 8821 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga 8881 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga 8941 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg aaaaaggatc ttcacctaga 9001 tccttttcac gtagaaagcc agtccgcaga aacggtgctg accccggatg aatgtcagct 9061 actgggctat ctggacaagg gaaaacgcaa gcgcaaagag aaagcaggta gcttgcagtg 9121 ggcttacatg gcgatagcta gactgggcgg ttttatggac agcaagcgaa ccggaattgc 9181 cagctggggc gccctctggt aaggttggga agccctgcaa agtaaactgg atggctttct 9241 cgccgccaag gatctgatgg cgcaggggat caagctctga tcaagagaca ggatgaggat 9301 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga 9361 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc 9421 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga 9481 atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg 9541 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc 9601 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg 9661 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga 9721 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc 9781 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca 9841 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg 9901 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct 9961 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg 10021 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc 10081 gccttcttga cgagttcttc tgaattttgt taaaattttt gttaaatcag ctcatttttt 10141 aaccaatagg ccgaaatcgg caacatccct tataaatcaa aagaatagac cgcgataggg 10201 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc 10261 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc acccaaatca 10321 agttttttgc ggtcgaggtg ccgtaaagct ctaaatcgga accctaaagg gagcccccga 10381 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa 10441 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc 10501 gcgcgcttaa tgcgccgcta cagggcgcgt ccattcgcca ttcaggatcg aattaattct 10561 taattaacat catcaataat atacctt
LOCUS dna 10587 bp FEATURES Location/Qualifiers Other Gene 162..310 /gene="Encap other" Promoter 365..393 /gene="amp prom" misc_binding 457..462 /dbxref="REBASE:HpaI" misc_binding 457..462 /dbxref="REBASE:HincII" Other Gene 715..895 /gene="HIV-1_5_LTR other" Other Gene 1006..1050 /gene="HIV-1_psi_pack other" misc_binding 1092..1097 /dbxref="REBASE:NruI" Regulatory_Seq 1557..1790 /gene="RRE reg" ORF 1600..2301 /sequence="ORF_1 rf(1)" misc_binding 2611..2616 /dbxref="REBASE:NdeI" Promoter 2885..2966 /gene="CMV prom" Regulatory_Seq 3022..3061 /gene="tetO reg" ORF 3063..3749 /sequence="ORF_6 rf(5)" misc_binding 3074..3079 /dbxref="REBASE:ClaI" misc_binding 3098..3103 /dbxref="REBASE:KpnI" misc_binding 3125..3130 /dbxref="REBASE:EcoRI" ORF 3135..6533 /sequence="ORF_3 rf(3)" ORF 4170..5390 /sequence="ORF_5 rf(5)" misc_binding 5099..5104 /dbxref="REBASE:XhoI" misc_binding 5187..5192 /dbxref="REBASE:EcoRV" misc_binding 5623..5628 /dbxref="REBASE:BamHI" ORF 6533..7171 /sequence="ORF_2 rf(2)" misc_binding 6540..6547 /dbxref="REBASE:NotI" Other Gene 7160..7212 /gene="delta_U3 other" Other Gene 7213..7393 /gene="HIV-1_5_LTR other" Rep_Origin 7899..8518 /gene="pBR322 origin" Promoter 9171..9220 /gene="NEOKAN prom" misc_binding 9278..9283 /dbxref="REBASE:BclI" ORF 9309..10103 /sequence="ORF_4 rf(3)" Marker 9312..10100 /gene="NTP_II marker" Rep_Origin 10208..10513 /gene="f1 origin" BASE COUNT 2430 a 2812 c 3002 g 2343 t 0 others ORIGIN 1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt 61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa 121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg 181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt 241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg 301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag 361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta 481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag 541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg 601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact 661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct 721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag 1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta 1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga 1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg 1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg 1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc 1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg 1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat 1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac 1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat 1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca 2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga 2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg 2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa 2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga 2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag 2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa 2401 aattcaaaat tttcgggttt ttcgaaccta gggttccgcg ttacataact tacggtaaat 2461 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt 2521 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa 2581 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc 2641 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct 2701 acttggcagt acatctacgt ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 2761 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 2821 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 2881 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 2941 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc 3001 atagaagaac cgagtttaaa ctccctatca gtgatagaga tctccctatc agtgatagag 3061 agctagcccc gggatcgatc aattgagtac ttacgtaggt accccagtgt ggtggcctgc 3121 aggtgaattc caccatgccg cgcgctcccc gctgccgagc cgtgcgctcc ctgctgcgca 3181 gccactaccg cgaggtgctg ccgctggcca cgttcgtgcg gcgcctgggg ccccagggct 3241 ggcggctggt gcagcgcggg gacccggcgg ctttccgcgc gctggtggcc cagtgcctgg 3301 tgtgcgtgcc ctgggacgca cggccgcccc ccgccgcccc ctccttccgc caggtgtcct 3361 gcctgaagga gctggtggcc cgagtgctgc agaggctgtg cgagcgcggc gcgaagaacg 3421 tgctggcctt cggcttcgcg ctgctggacg gggcccgcgg gggccccccc gaggccttca 3481 ccaccagcgt gcgcagctac ctgcccaaca cggtgaccga cgcactgcgg gggagcgggg 3541 cgtgggggct gctgctgcgc cgcgtgggcg acgacgtgct ggttcacctg ctggcacgct 3601 gcgcgctctt tgtgctggtg gctcccagct gcgcctacca ggtgtgcggg ccgccgctgt 3661 accagctcgg cgctgccact caggcccggc ccccgccaca cgctagtgga ccccgaaggc 3721 gtctgggatg cgaacgggcc tggaaccata gcgtcaggga ggccggggtc cccctgggcc 3781 tgccagcccc gggtgcgagg aggcgcgggg gcagtgccag ccgaagtctg ccgttgccca 3841 agaggcccag gcgtggcgct gcccctgagc cggagcggac gcccgttggg caggggtcct 3901 gggcccaccc gggcaggacg cgtggaccga gtgaccgtgg tttctgtgtg gtgtcacctg 3961 ccagacccgc cgaagaagcc acctctttgg agggtgcgct ctctggcacg cgccactccc 4021 acccatccgt gggccgccag caccacgcgg gccccccatc cacatcgcgg ccaccacgtc 4081 cctgggacac gccttgtccc ccggtgtacg ccgagaccaa gcacttcctc tactcctcag 4141 gcgacaagga gcagctgcgg ccctccttcc tactcagctc tctgaggccc agcctgactg 4201 gcgctcggag gctcgtggag accatctttc tgggttccag gccctggatg ccagggactc 4261 cccgcaggtt gccccgcctg ccccagcgct actggcaaat gcggcccctg tttctggagc 4321 tgcttgggaa ccacgcgcag tgcccctacg gggtgctcct caagacgcac tgcccgctgc 4381 gagctgcggt caccccagca gccggtgtct gtgcccggga gaagccccag ggctctgtgg 4441 cggcccccga ggaggaggac acagaccccc gtcgcctggt gcagctgctc cgccagcaca 4501 gcagcccctg gcaggtgtac ggcttcgtgc gggcctgcct gcgccggctg gtgcccccag 4561 gcctctgggg ctccaggcac aacgaacgcc gcttcctcag gaacaccaag aagttcatct 4621 ccctggggaa gcatgccaag ctctcgctgc aggagctgac gtggaagatg agcgtgcggg 4681 actgcgcttg gctgcgcagg agcccagggg ttggctgtgt tccggccgca gagcaccgtc 4741 tgcgtgagga gatcctggcc aagttcctgc actggctgat gagtgtgtac gtcgtcgagc 4801 tgctcaggtc tttcttttat gtcacggaga ccacgtttca aaagaacagg ctctttttct 4861 accggaagag tgtctggagc aagttgcaaa gcattggaat cagacagcac ttgaagaggg 4921 tgcagctgcg ggagctgtcg gaagcagagg tcaggcagca tcgggaagcc aggcccgccc 4981 tgctgacgtc cagactccgc ttcatcccca agcctgacgg gctgcggccg attgtgaaca 5041 tggactacgt cgtgggagcc agaacgttcc gcagagaaaa gagggccgag cgtctcacct 5101 cgagggtgaa ggcactgttc agcgtgctca actacgagcg ggcgcggcgc cccggcctcc 5161 tgggcgcctc tgtgctgggc ctggacgata tccacagggc ctggcgcacc ttcgtgctgc 5221 gtgtgcgggc ccaggacccg ccgcctgagc tgtactttgt caaggtggat gtgacgggcg 5281 cgtacgacac catcccccag gacaggctca cggaggtcat cgccagcatc atcaaacccc 5341 agaacacgta ctgcgtgcgt cggtatgccg tggtccagaa ggccgcccat gggcacgtcc 5401 gcaaggcctt caagagccac gtctctacct tgacagacct ccagccgtac atgcgacagt 5461 tcgtggctca cctgcaggag accagcccgc tgagggatgc cgtcgtcatc gagcagagct 5521 cctccctgaa tgaggccagc agtggcctct tcgacgtctt cctacgcttc atgtgccacc 5581 acgccgtgcg catcaggggc aagtcctacg tccagtgcca ggggatcccg cagggctcca 5641 tcctctccac gctgctctgc agcctgtgct acggcgacat ggagaacaag ctgtttgcgg 5701 ggattcggcg ggacgggctg ctcctgcgtt tggtggatga tttcttgttg gtgacacctc 5761 acctcaccca cgcgaaaacc ttcctcagga ccctggtccg aggtgtccct gagtatggct 5821 gcgtggtgaa cttgcggaag acagtggtga acttccctgt agaagacgag gccctgggtg 5881 gcacggcttt tgttcagatg ccggcccacg gcctattccc ctggtgcggc ctgctgctgg 5941 atacccggac cctggaggtg cagagcgact actccagcta tgcccggacc tccatcagag 6001 ccagtctcac cttcaaccgc ggcttcaagg ctgggaggaa catgcgtcgc aaactctttg 6061 gggtcttgcg gctgaagtgt cacagcctgt ttctggattt gcaggtgaac agcctccaga 6121 cggtgtgcac caacatctac aagatcctcc tgctgcaggc gtacaggttt cacgcatgtg 6181 tgctgcagct cccatttcat cagcaagttt ggaagaaccc cacatttttc ctgcgcgtca 6241 tctctgacac ggcctccctc tgctactcca tcctgaaagc caagaacgca gggatgtcgc 6301 tgggggccaa gggcgccgcc ggccctctgc cctccgaggc cgtgcagtgg ctgtgccacc 6361 aagcattcct gctcaagctg actcgacacc gtgtcaccta cgtgccactc ctggggtcac 6421 tcaggacagc ccagacgcag ctgagtcgga agctcccggg gacgacgctg actgccctgg 6481 aggccgcagc caacccggca ctgccctcag acttcaagac catcctggac tgatgggacg 6541 cggccgcatg actagttccg gaaatcaacc tctggattac aaaatttgtg aaagattgac 6601 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt 6661 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt 6721 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt 6781 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg 6841 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg 6901 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct 6961 gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt 7021 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc 7081 tctgcggcct cttccgcgtc tcgccttcgc cctcagacga gtcggatctc cctttgggcc 7141 gcctccccgc ctgtccggat ggaagggcta attcactccc aacgaataca agatctgctt 7201 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa 7261 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt 7321 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg 7381 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag 7441 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa 7501 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt 7561 ttgtccaaac tcatcaatgt atcttatcat gtctggcatc tatgtcgggt gcggagaaag 7621 aggtaatgaa atggcattat gggtattatg ggtctgcatt aatgaatcgg ccaacgatcc 7681 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct 7741 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac 7801 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga 7861 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat 7921 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac 7981 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct 8041 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg 8101 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg 8161 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt 8221 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg 8281 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac 8341 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga 8401 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt 8461 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt 8521 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga 8581 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc 8641 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct 8701 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata 8761 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca 8821 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga 8881 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga 8941 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg aaaaaggatc ttcacctaga 9001 tccttttcac gtagaaagcc agtccgcaga aacggtgctg accccggatg aatgtcagct 9061 actgggctat ctggacaagg gaaaacgcaa gcgcaaagag aaagcaggta gcttgcagtg 9121 ggcttacatg gcgatagcta gactgggcgg ttttatggac agcaagcgaa ccggaattgc 9181 cagctggggc gccctctggt aaggttggga agccctgcaa agtaaactgg atggctttct 9241 cgccgccaag gatctgatgg cgcaggggat caagctctga tcaagagaca ggatgaggat 9301 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga 9361 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc 9421 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga 9481 atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg 9541 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc 9601 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg 9661 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga 9721 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc 9781 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca 9841 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg 9901 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct 9961 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg 10021 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc 10081 gccttcttga cgagttcttc tgaattttgt taaaattttt gttaaatcag ctcatttttt 10141 aaccaatagg ccgaaatcgg caacatccct tataaatcaa aagaatagac cgcgataggg 10201 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc 10261 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc acccaaatca 10321 agttttttgc ggtcgaggtg ccgtaaagct ctaaatcgga accctaaagg gagcccccga 10381 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa 10441 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc 10501 gcgcgcttaa tgcgccgcta cagggcgcgt ccattcgcca ttcaggatcg aattaattct 10561 taattaacat catcaataat atacctt //
***Note: The text map shown below is representative of the parental
vector only and does not include the gene of interest. The sequence of the gene
can be found through the accession number link on the product page.
1 ttttggattgaagccaatatgataatgagggggtggagtttgtgacgtggcgcggggcgt 60 AAAACCTAACTTCGGTTATACTATTACTCCCCCACCTCAAACACTGCACCGCGCCCCGCA 61 gggaacggggcgggtgacgtagtagtgtggcggaagtgtgatgttgcaagtgtggcggaa 120 CCCTTGCCCCGCCCACTGCATCATCACACCGCCTTCACACTACAACGTTCACACCGCCTT Encap other(162,310)>>> | 121 cacatgtaagcgacggatgtggcaaaagtgacgtttttggtgtgcgccggtgtacacagg 180 GTGTACATTCGCTGCCTACACCGTTTTCACTGCAAAAACCACACGCGGCCACATGTGTCC 181 aagtgacaattttcgcgcggttttaggcggatgttgtagtaaatttgggcgtaaccgagt 240 TTCACTGTTAAAAGCGCGCCAAAATCCGCCTACAACATCATTTAAACCCGCATTGGCTCA 241 aagatttggccattttcgcgggaaaactgaataagaggaagtgaaatctgaataattttg 300 TTCTAAACCGGTAAAAGCGCCCTTTTGACTTATTCTCCTTCACTTTAGACTTATTAAAAC 301 tgttactcatagcgcgtaatacggcagacctcagcgctagattattgaagcatttatcag 360 ACAATGAGTATCGCGCATTATGCCGTCTGGAGTCGCGATCTAATAACTTCGTAAATAGTC amp prom(365,393)<<< | 361 ggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggg 420 CCAATAACAGAGTACTCGCCTATGTATAAACTTACATAAATCTTTTTATTTGTTTATCCC HincII | HpaI | 421 gttccgcgcacatttccccgaaaagtgccacctgacgttaactataacggtcctaaggta 480 CAAGGCGCGTGTAAAGGGGCTTTTCACGGTGGACTGCAATTGATATTGCCAGGATTCCAT 481 gcgaaaatgtagtcttatgcaatactcttgtagtcttgcaacatggtaacgatgagttag 540 CGCTTTTACATCAGAATACGTTATGAGAACATCAGAACGTTGTACCATTGCTACTCAATC 541 caacatgccttacaaggagagaaaaagcaccgtgcatgccgattggtggaagtaaggtgg 600 GTTGTACGGAATGTTCCTCTCTTTTTCGTGGCACGTACGGCTAACCACCTTCATTCCACC 601 tacgatcgtgccttattaggaaggcaacagacgggtctgacatggattggacgaaccact 660 ATGCTAGCACGGAATAATCCTTCCGTTGTCTGCCCAGACTGTACCTAACCTGCTTGGTGA HIV-1_5_LTR other(715,895)>>> | 661 gaattgccgcattgcagagatattgtatttaagtgcctagctcgatacataaacgggtct 720 CTTAACGGCGTAACGTCTCTATAACATAAATTCACGGATCGAGCTATGTATTTGCCCAGA 721 ctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgctt 780 GAGACCAATCTGGTCTAGACTCGGACCCTCGAGAGACCGATTGATCCCTTGGGTGACGAA 781 aagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgac 840 TTCGGAGTTATTTCGAACGGAACTCACGAAGTTCATCACACACGGGCAGACAACACACTG 841 tctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtggc 900 AGACCATTGATCTCTAGGGAGTCTGGGAAAATCAGTCACACCTTTTAGAGATCGTCACCG 901 gcccgaacagggacttgaaagcgaaagggaaaccagaggagctctctcgacgcaggactc 960 CGGGCTTGTCCCTGAACTTTCGCTTTCCCTTTGGTCTCCTCGAGAGAGCTGCGTCCTGAG HIV-1_psi_pack other(1006,1050)>>> | 961 ggcttgctgaagcgcgcacggcaagaggcgaggggcggcgactggtgagtacgccaaaaa 1020 CCGAACGACTTCGCGCGTGCCGTTCTCCGCTCCCCGCCGCTGACCACTCATGCGGTTTTT 1021 ttttgactagcggaggctagaaggagagagatgggtgcgagagcgtcagtattaagcggg 1080 AAAACTGATCGCCTCCGATCTTCCTCTCTCTACCCACGCTCTCGCAGTCATAATTCGCCC NruI | 1081 ggagaattagatcgcgatgggaaaaaattcggttaaggccagggggaaagaaaaaatata 1140 CCTCTTAATCTAGCGCTACCCTTTTTTAAGCCAATTCCGGTCCCCCTTTCTTTTTTATAT 1141 aattaaaacatatagtatgggcaagcagggagctagaacgattcgcagttaatcctggcc 1200 TTAATTTTGTATATCATACCCGTTCGTCCCTCGATCTTGCTAAGCGTCAATTAGGACCGG 1201 tgttagaaacatcagaaggctgtagacaaatactgggacagctacaaccatcccttcaga 1260 ACAATCTTTGTAGTCTTCCGACATCTGTTTATGACCCTGTCGATGTTGGTAGGGAAGTCT 1261 caggatcagaagaacttagatcattatataatacagtagcaaccctctattgtgtgcatc 1320 GTCCTAGTCTTCTTGAATCTAGTAATATATTATGTCATCGTTGGGAGATAACACACGTAG 1321 aaaggatagagataaaagacaccaaggaagctttagacaagatagaggaagagcaaaaca 1380 TTTCCTATCTCTATTTTCTGTGGTTCCTTCGAAATCTGTTCTATCTCCTTCTCGTTTTGT 1381 aaagtaagaccaccgcacagcaagcccgctgatcttcagacctggaggaggagatatgag 1440 TTTCATTCTGGTGGCGTGTCGTTCGGGCGACTAGAAGTCTGGACCTCCTCCTCTATACTC 1441 ggacattggagaagtgaattatataaatataaagtagtaaaaattgaaccattaggagta 1500 CCTGTAACCTCTTCACTTAATATATTTATATTTCATCATTTTTAACTTGGTAATCCTCAT RRE reg(1557,1790)>>> | 1501 gcacccaccaaggcaaagagaagagtggtgcagagagaaaaaagagcagtgggaatagga 1560 CGTGGGTGGTTCCGTTTCTCTTCTCACCACGTCTCTCTTTTTTCTCGTCACCCTTATCCT ORF_1 rf(1)(1600,2301)>>> | 1561 gctttgttccttgggttcttgggagcagcaggaagcactatgggcgcagcgtcaatgacg 1620 CGAAACAAGGAACCCAAGAACCCTCGTCGTCCTTCGTGATACCCGCGTCGCAGTTACTGC 1621 ctgacggtacaggccagacaattattgtctggtatagtgcagcagcagaacaatttgctg 1680 GACTGCCATGTCCGGTCTGTTAATAACAGACCATATCACGTCGTCGTCTTGTTAAACGAC 1681 agggctattgaggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctc 1740 TCCCGATAACTCCGCGTTGTCGTAGACAACGTTGAGTGTCAGACCCCGTAGTTCGTCGAG 1741 caggcaagaatcctggctgtggaaagatacctaaaggatcaacagctcctggggatttgg 1800 GTCCGTTCTTAGGACCGACACCTTTCTATGGATTTCCTAGTTGTCGAGGACCCCTAAACC 1801 ggttgctctggaaaactcatttgcaccactgctgtgccttggaatgctagttggagtaat 1860 CCAACGAGACCTTTTGAGTAAACGTGGTGACGACACGGAACCTTACGATCAACCTCATTA 1861 aaatctctggaacagatttggaatcacacgacctggatggagtgggacagagaaattaac 1920 TTTAGAGACCTTGTCTAAACCTTAGTGTGCTGGACCTACCTCACCCTGTCTCTTTAATTG 1921 aattacacaagcttaatacactccttaattgaagaatcgcaaaaccagcaagaaaagaat 1980 TTAATGTGTTCGAATTATGTGAGGAATTAACTTCTTAGCGTTTTGGTCGTTCTTTTCTTA 1981 gaacaagaattattggaattagataaatgggcaagtttgtggaattggtttaacataaca 2040 CTTGTTCTTAATAACCTTAATCTATTTACCCGTTCAAACACCTTAACCAAATTGTATTGT 2041 aattggctgtggtatataaaattattcataatgatagtaggaggcttggtaggtttaaga 2100 TTAACCGACACCATATATTTTAATAAGTATTACTATCATCCTCCGAACCATCCAAATTCT 2101 atagtttttgctgtactttctatagtgaatagagttaggcagggatattcaccattatcg 2160 TATCAAAAACGACATGAAAGATATCACTTATCTCAATCCGTCCCTATAAGTGGTAATAGC 2161 tttcagacccacctcccaaccccgaggggacccgacaggcccgaaggaatagaagaagaa 2220 AAAGTCTGGGTGGAGGGTTGGGGCTCCCCTGGGCTGTCCGGGCTTCCTTATCTTCTTCTT 2221 ggtggagagagagacagagacagatccattcgattagtgaacggatctcgacggtatcga 2280 CCACCTCTCTCTCTGTCTCTGTCTAGGTAAGCTAATCACTTGCCTAGAGCTGCCATAGCT 2281 aagcttgggattcgaatttaaaagaaaaggggggattggggggtacagtgcaggggaaag 2340 TTCGAACCCTAAGCTTAAATTTTCTTTTCCCCCCTAACCCCCCATGTCACGTCCCCTTTC 2341 aatagtagacataatagcaacagacatacaaactaaagaactacaaaaacaaattacaaa 2400 TTATCATCTGTATTATCGTTGTCTGTATGTTTGATTTCTTGATGTTTTTGTTTAATGTTT 2401 aattcaaaattttcgggtttttcgaacctagggttccgcgttacataacttacggtaaat 2460 TTAAGTTTTAAAAGCCCAAAAAGCTTGGATCCCAAGGCGCAATGTATTGAATGCCATTTA 2461 ggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgtt 2520 CCGGGCGGACCGACTGGCGGGTTGCTGGGGGCGGGTAACTGCAGTTATTACTGCATACAA 2521 cccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaa 2580 GGGTATCATTGCGGTTATCCCTGAAAGGTAACTGCAGTTACCCACCTCATAAATGCCATT NdeI | 2581 actgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtc 2640 TGACGGGTGAACCGTCATGTAGTTCACATAGTATACGGTTCATGCGGGGGATAACTGCAG 2641 aatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcct 2700 TTACTGCCATTTACCGGGCGGACCGTAATACGGGTCATGTACTGGAATACCCTGAAAGGA 2701 acttggcagtacatctacgtttagtcatcgctattaccatggtgatgcggttttggcagt 2760 TGAACCGTCATGTAGATGCAAATCAGTAGCGATAATGGTACCACTACGCCAAAACCGTCA 2761 acatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattg 2820 TGTAGTTACCCGCACCTATCGCCAAACTGAGTGCCCCTAAAGGTTCAGAGGTGGGGTAAC 2821 acgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaaca 2880 TGCAGTTACCCTCAAACAAAACCGTGGTTTTAGTTGCCCTGAAAGGTTTTACAGCATTGT CMV prom(2885,2966)>>> | 2881 actccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagca 2940 TGAGGCGGGGTAACTGCGTTTACCCGCCATCCGCACATGCCACCCTCCAGATATATTCGT 2941 gagctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttttgacctcc 3000 CTCGAGCAAATCACTTGGCAGTCTAGCGGACCTCTGCGGTAGGTGCGACAAAACTGGAGG tetO reg(3022,3061)>>> | 3001 atagaagaaccgagtttaaactccctatcagtgatagagatctccctatcagtgatagag 3060 TATCTTCTTGGCTCAAATTTGAGGGATAGTCACTATCTCTAGAGGGATAGTCACTATCTC ClaI | ORF_6 rf(5)(3063,3749)<<< KpnI | | | 3061 agctagccccgggatcgatcaattgagtacttacgtaggtaccccagtgtggtggcctgc 3120 TCGATCGGGGCCCTAGCTAGTTAACTCATGAATGCATCCATGGGGTCACACCACCGGACG EcoRI ORF_3 rf(3)(3135,6533)>>> | | 3121 aggtgaattccaccatgccgcgcgctccccgctgccgagccgtgcgctccctgctgcgca 3180 TCCACTTAAGGTGGTACGGCGCGCGAGGGGCGACGGCTCGGCACGCGAGGGACGACGCGT 3181 gccactaccgcgaggtgctgccgctggccacgttcgtgcggcgcctggggccccagggct 3240 CGGTGATGGCGCTCCACGACGGCGACCGGTGCAAGCACGCCGCGGACCCCGGGGTCCCGA 3241 ggcggctggtgcagcgcggggacccggcggctttccgcgcgctggtggcccagtgcctgg 3300 CCGCCGACCACGTCGCGCCCCTGGGCCGCCGAAAGGCGCGCGACCACCGGGTCACGGACC 3301 tgtgcgtgccctgggacgcacggccgccccccgccgccccctccttccgccaggtgtcct 3360 ACACGCACGGGACCCTGCGTGCCGGCGGGGGGCGGCGGGGGAGGAAGGCGGTCCACAGGA 3361 gcctgaaggagctggtggcccgagtgctgcagaggctgtgcgagcgcggcgcgaagaacg 3420 CGGACTTCCTCGACCACCGGGCTCACGACGTCTCCGACACGCTCGCGCCGCGCTTCTTGC 3421 tgctggccttcggcttcgcgctgctggacggggcccgcgggggcccccccgaggccttca 3480 ACGACCGGAAGCCGAAGCGCGACGACCTGCCCCGGGCGCCCCCGGGGGGGCTCCGGAAGT 3481 ccaccagcgtgcgcagctacctgcccaacacggtgaccgacgcactgcgggggagcgggg 3540 GGTGGTCGCACGCGTCGATGGACGGGTTGTGCCACTGGCTGCGTGACGCCCCCTCGCCCC 3541 cgtgggggctgctgctgcgccgcgtgggcgacgacgtgctggttcacctgctggcacgct 3600 GCACCCCCGACGACGACGCGGCGCACCCGCTGCTGCACGACCAAGTGGACGACCGTGCGA 3601 gcgcgctctttgtgctggtggctcccagctgcgcctaccaggtgtgcgggccgccgctgt 3660 CGCGCGAGAAACACGACCACCGAGGGTCGACGCGGATGGTCCACACGCCCGGCGGCGACA 3661 accagctcggcgctgccactcaggcccggcccccgccacacgctagtggaccccgaaggc 3720 TGGTCGAGCCGCGACGGTGAGTCCGGGCCGGGGGCGGTGTGCGATCACCTGGGGCTTCCG 3721 gtctgggatgcgaacgggcctggaaccatagcgtcagggaggccggggtccccctgggcc 3780 CAGACCCTACGCTTGCCCGGACCTTGGTATCGCAGTCCCTCCGGCCCCAGGGGGACCCGG 3781 tgccagccccgggtgcgaggaggcgcgggggcagtgccagccgaagtctgccgttgccca 3840 ACGGTCGGGGCCCACGCTCCTCCGCGCCCCCGTCACGGTCGGCTTCAGACGGCAACGGGT 3841 agaggcccaggcgtggcgctgcccctgagccggagcggacgcccgttgggcaggggtcct 3900 TCTCCGGGTCCGCACCGCGACGGGGACTCGGCCTCGCCTGCGGGCAACCCGTCCCCAGGA 3901 gggcccacccgggcaggacgcgtggaccgagtgaccgtggtttctgtgtggtgtcacctg 3960 CCCGGGTGGGCCCGTCCTGCGCACCTGGCTCACTGGCACCAAAGACACACCACAGTGGAC 3961 ccagacccgccgaagaagccacctctttggagggtgcgctctctggcacgcgccactccc 4020 GGTCTGGGCGGCTTCTTCGGTGGAGAAACCTCCCACGCGAGAGACCGTGCGCGGTGAGGG 4021 acccatccgtgggccgccagcaccacgcgggccccccatccacatcgcggccaccacgtc 4080 TGGGTAGGCACCCGGCGGTCGTGGTGCGCCCGGGGGGTAGGTGTAGCGCCGGTGGTGCAG 4081 cctgggacacgccttgtcccccggtgtacgccgagaccaagcacttcctctactcctcag 4140 GGACCCTGTGCGGAACAGGGGGCCACATGCGGCTCTGGTTCGTGAAGGAGATGAGGAGTC ORF_5 rf(5)(4170,5390)<<< | 4141 gcgacaaggagcagctgcggccctccttcctactcagctctctgaggcccagcctgactg 4200 CGCTGTTCCTCGTCGACGCCGGGAGGAAGGATGAGTCGAGAGACTCCGGGTCGGACTGAC 4201 gcgctcggaggctcgtggagaccatctttctgggttccaggccctggatgccagggactc 4260 CGCGAGCCTCCGAGCACCTCTGGTAGAAAGACCCAAGGTCCGGGACCTACGGTCCCTGAG 4261 cccgcaggttgccccgcctgccccagcgctactggcaaatgcggcccctgtttctggagc 4320 GGGCGTCCAACGGGGCGGACGGGGTCGCGATGACCGTTTACGCCGGGGACAAAGACCTCG 4321 tgcttgggaaccacgcgcagtgcccctacggggtgctcctcaagacgcactgcccgctgc 4380 ACGAACCCTTGGTGCGCGTCACGGGGATGCCCCACGAGGAGTTCTGCGTGACGGGCGACG 4381 gagctgcggtcaccccagcagccggtgtctgtgcccgggagaagccccagggctctgtgg 4440 CTCGACGCCAGTGGGGTCGTCGGCCACAGACACGGGCCCTCTTCGGGGTCCCGAGACACC 4441 cggcccccgaggaggaggacacagacccccgtcgcctggtgcagctgctccgccagcaca 4500 GCCGGGGGCTCCTCCTCCTGTGTCTGGGGGCAGCGGACCACGTCGACGAGGCGGTCGTGT 4501 gcagcccctggcaggtgtacggcttcgtgcgggcctgcctgcgccggctggtgcccccag 4560 CGTCGGGGACCGTCCACATGCCGAAGCACGCCCGGACGGACGCGGCCGACCACGGGGGTC 4561 gcctctggggctccaggcacaacgaacgccgcttcctcaggaacaccaagaagttcatct 4620 CGGAGACCCCGAGGTCCGTGTTGCTTGCGGCGAAGGAGTCCTTGTGGTTCTTCAAGTAGA 4621 ccctggggaagcatgccaagctctcgctgcaggagctgacgtggaagatgagcgtgcggg 4680 GGGACCCCTTCGTACGGTTCGAGAGCGACGTCCTCGACTGCACCTTCTACTCGCACGCCC 4681 actgcgcttggctgcgcaggagcccaggggttggctgtgttccggccgcagagcaccgtc 4740 TGACGCGAACCGACGCGTCCTCGGGTCCCCAACCGACACAAGGCCGGCGTCTCGTGGCAG 4741 tgcgtgaggagatcctggccaagttcctgcactggctgatgagtgtgtacgtcgtcgagc 4800 ACGCACTCCTCTAGGACCGGTTCAAGGACGTGACCGACTACTCACACATGCAGCAGCTCG 4801 tgctcaggtctttcttttatgtcacggagaccacgtttcaaaagaacaggctctttttct 4860 ACGAGTCCAGAAAGAAAATACAGTGCCTCTGGTGCAAAGTTTTCTTGTCCGAGAAAAAGA 4861 accggaagagtgtctggagcaagttgcaaagcattggaatcagacagcacttgaagaggg 4920 TGGCCTTCTCACAGACCTCGTTCAACGTTTCGTAACCTTAGTCTGTCGTGAACTTCTCCC 4921 tgcagctgcgggagctgtcggaagcagaggtcaggcagcatcgggaagccaggcccgccc 4980 ACGTCGACGCCCTCGACAGCCTTCGTCTCCAGTCCGTCGTAGCCCTTCGGTCCGGGCGGG 4981 tgctgacgtccagactccgcttcatccccaagcctgacgggctgcggccgattgtgaaca 5040 ACGACTGCAGGTCTGAGGCGAAGTAGGGGTTCGGACTGCCCGACGCCGGCTAACACTTGT 5041 tggactacgtcgtgggagccagaacgttccgcagagaaaagagggccgagcgtctcacct 5100 ACCTGATGCAGCACCCTCGGTCTTGCAAGGCGTCTCTTTTCTCCCGGCTCGCAGAGTGGA XhoI | 5101 cgagggtgaaggcactgttcagcgtgctcaactacgagcgggcgcggcgccccggcctcc 5160 GCTCCCACTTCCGTGACAAGTCGCACGAGTTGATGCTCGCCCGCGCCGCGGGGCCGGAGG EcoRV | 5161 tgggcgcctctgtgctgggcctggacgatatccacagggcctggcgcaccttcgtgctgc 5220 ACCCGCGGAGACACGACCCGGACCTGCTATAGGTGTCCCGGACCGCGTGGAAGCACGACG 5221 gtgtgcgggcccaggacccgccgcctgagctgtactttgtcaaggtggatgtgacgggcg 5280 CACACGCCCGGGTCCTGGGCGGCGGACTCGACATGAAACAGTTCCACCTACACTGCCCGC 5281 cgtacgacaccatcccccaggacaggctcacggaggtcatcgccagcatcatcaaacccc 5340 GCATGCTGTGGTAGGGGGTCCTGTCCGAGTGCCTCCAGTAGCGGTCGTAGTAGTTTGGGG 5341 agaacacgtactgcgtgcgtcggtatgccgtggtccagaaggccgcccatgggcacgtcc 5400 TCTTGTGCATGACGCACGCAGCCATACGGCACCAGGTCTTCCGGCGGGTACCCGTGCAGG 5401 gcaaggccttcaagagccacgtctctaccttgacagacctccagccgtacatgcgacagt 5460 CGTTCCGGAAGTTCTCGGTGCAGAGATGGAACTGTCTGGAGGTCGGCATGTACGCTGTCA 5461 tcgtggctcacctgcaggagaccagcccgctgagggatgccgtcgtcatcgagcagagct 5520 AGCACCGAGTGGACGTCCTCTGGTCGGGCGACTCCCTACGGCAGCAGTAGCTCGTCTCGA 5521 cctccctgaatgaggccagcagtggcctcttcgacgtcttcctacgcttcatgtgccacc 5580 GGAGGGACTTACTCCGGTCGTCACCGGAGAAGCTGCAGAAGGATGCGAAGTACACGGTGG BamHI | 5581 acgccgtgcgcatcaggggcaagtcctacgtccagtgccaggggatcccgcagggctcca 5640 TGCGGCACGCGTAGTCCCCGTTCAGGATGCAGGTCACGGTCCCCTAGGGCGTCCCGAGGT 5641 tcctctccacgctgctctgcagcctgtgctacggcgacatggagaacaagctgtttgcgg 5700 AGGAGAGGTGCGACGAGACGTCGGACACGATGCCGCTGTACCTCTTGTTCGACAAACGCC 5701 ggattcggcgggacgggctgctcctgcgtttggtggatgatttcttgttggtgacacctc 5760 CCTAAGCCGCCCTGCCCGACGAGGACGCAAACCACCTACTAAAGAACAACCACTGTGGAG 5761 acctcacccacgcgaaaaccttcctcaggaccctggtccgaggtgtccctgagtatggct 5820 TGGAGTGGGTGCGCTTTTGGAAGGAGTCCTGGGACCAGGCTCCACAGGGACTCATACCGA 5821 gcgtggtgaacttgcggaagacagtggtgaacttccctgtagaagacgaggccctgggtg 5880 CGCACCACTTGAACGCCTTCTGTCACCACTTGAAGGGACATCTTCTGCTCCGGGACCCAC 5881 gcacggcttttgttcagatgccggcccacggcctattcccctggtgcggcctgctgctgg 5940 CGTGCCGAAAACAAGTCTACGGCCGGGTGCCGGATAAGGGGACCACGCCGGACGACGACC 5941 atacccggaccctggaggtgcagagcgactactccagctatgcccggacctccatcagag 6000 TATGGGCCTGGGACCTCCACGTCTCGCTGATGAGGTCGATACGGGCCTGGAGGTAGTCTC 6001 ccagtctcaccttcaaccgcggcttcaaggctgggaggaacatgcgtcgcaaactctttg 6060 GGTCAGAGTGGAAGTTGGCGCCGAAGTTCCGACCCTCCTTGTACGCAGCGTTTGAGAAAC 6061 gggtcttgcggctgaagtgtcacagcctgtttctggatttgcaggtgaacagcctccaga 6120 CCCAGAACGCCGACTTCACAGTGTCGGACAAAGACCTAAACGTCCACTTGTCGGAGGTCT 6121 cggtgtgcaccaacatctacaagatcctcctgctgcaggcgtacaggtttcacgcatgtg 6180 GCCACACGTGGTTGTAGATGTTCTAGGAGGACGACGTCCGCATGTCCAAAGTGCGTACAC 6181 tgctgcagctcccatttcatcagcaagtttggaagaaccccacatttttcctgcgcgtca 6240 ACGACGTCGAGGGTAAAGTAGTCGTTCAAACCTTCTTGGGGTGTAAAAAGGACGCGCAGT 6241 tctctgacacggcctccctctgctactccatcctgaaagccaagaacgcagggatgtcgc 6300 AGAGACTGTGCCGGAGGGAGACGATGAGGTAGGACTTTCGGTTCTTGCGTCCCTACAGCG 6301 tgggggccaagggcgccgccggccctctgccctccgaggccgtgcagtggctgtgccacc 6360 ACCCCCGGTTCCCGCGGCGGCCGGGAGACGGGAGGCTCCGGCACGTCACCGACACGGTGG 6361 aagcattcctgctcaagctgactcgacaccgtgtcacctacgtgccactcctggggtcac 6420 TTCGTAAGGACGAGTTCGACTGAGCTGTGGCACAGTGGATGCACGGTGAGGACCCCAGTG 6421 tcaggacagcccagacgcagctgagtcggaagctcccggggacgacgctgactgccctgg 6480 AGTCCTGTCGGGTCTGCGTCGACTCAGCCTTCGAGGGCCCCTGCTGCGACTGACGGGACC ORF_2 rf(2)(6533,7171)>>> | 6481 aggccgcagccaacccggcactgccctcagacttcaagaccatcctggactgatgggacg 6540 TCCGGCGTCGGTTGGGCCGTGACGGGAGTCTGAAGTTCTGGTAGGACCTGACTACCCTGC NotI | 6541 cggccgcatgactagttccggaaatcaacctctggattacaaaatttgtgaaagattgac 6600 GCCGGCGTACTGATCAAGGCCTTTAGTTGGAGACCTAATGTTTTAAACACTTTCTAACTG 6601 tggtattcttaactatgttgctccttttacgctatgtggatacgctgctttaatgccttt 6660 ACCATAAGAATTGATACAACGAGGAAAATGCGATACACCTATGCGACGAAATTACGGAAA 6661 gtatcatgctattgcttcccgtatggctttcattttctcctccttgtataaatcctggtt 6720 CATAGTACGATAACGAAGGGCATACCGAAAGTAAAAGAGGAGGAACATATTTAGGACCAA 6721 gctgtctctttatgaggagttgtggcccgttgtcaggcaacgtggcgtggtgtgcactgt 6780 CGACAGAGAAATACTCCTCAACACCGGGCAACAGTCCGTTGCACCGCACCACACGTGACA 6781 gtttgctgacgcaacccccactggttggggcattgccaccacctgtcagctcctttccgg 6840 CAAACGACTGCGTTGGGGGTGACCAACCCCGTAACGGTGGTGGACAGTCGAGGAAAGGCC 6841 gactttcgctttccccctccctattgccacggcggaactcatcgccgcctgccttgcccg 6900 CTGAAAGCGAAAGGGGGAGGGATAACGGTGCCGCCTTGAGTAGCGGCGGACGGAACGGGC 6901 ctgctggacaggggctcggctgttgggcactgacaattccgtggtgttgtcggggaagct 6960 GACGACCTGTCCCCGAGCCGACAACCCGTGACTGTTAAGGCACCACAACAGCCCCTTCGA 6961 gacgtcctttccatggctgctcgcctgtgttgccacctggattctgcgcgggacgtcctt 7020 CTGCAGGAAAGGTACCGACGAGCGGACACAACGGTGGACCTAAGACGCGCCCTGCAGGAA 7021 ctgctacgtcccttcggccctcaatccagcggaccttccttcccgcggcctgctgccggc 7080 GACGATGCAGGGAAGCCGGGAGTTAGGTCGCCTGGAAGGAAGGGCGCCGGACGACGGCCG 7081 tctgcggcctcttccgcgtctcgccttcgccctcagacgagtcggatctccctttgggcc 7140 AGACGCCGGAGAAGGCGCAGAGCGGAAGCGGGAGTCTGCTCAGCCTAGAGGGAAACCCGG delta_U3 other(7160,7212)>>> | 7141 gcctccccgcctgtccggatggaagggctaattcactcccaacgaatacaagatctgctt 7200 CGGAGGGGCGGACAGGCCTACCTTCCCGATTAAGTGAGGGTTGCTTATGTTCTAGACGAA HIV-1_5_LTR other(7213,7393)>>> | 7201 tttgcttgtactgggtctctctggttagaccagatctgagcctgggagctctctggctaa 7260 AAACGAACATGACCCAGAGAGACCAATCTGGTCTAGACTCGGACCCTCGAGAGACCGATT 7261 ctagggaacccactgcttaagcctcaataaagcttgccttgagtgcttcaagtagtgtgt 7320 GATCCCTTGGGTGACGAATTCGGAGTTATTTCGAACGGAACTCACGAAGTTCATCACACA 7321 gcccgtctgttgtgtgactctggtaactagagatccctcagacccttttagtcagtgtgg 7380 CGGGCAGACAACACACTGAGACCATTGATCTCTAGGGAGTCTGGGAAAATCAGTCACACC 7381 aaaatctctagcagtagtagttcatgtcatcttattattcagtatttataacttgcaaag 7440 TTTTAGAGATCGTCATCATCAAGTACAGTAGAATAATAAGTCATAAATATTGAACGTTTC 7441 aaatgaatatcagagagtgagaggaacttgtttattgcagcttataatggttacaaataa 7500 TTTACTTATAGTCTCTCACTCTCCTTGAACAAATAACGTCGAATATTACCAATGTTTATT 7501 agcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggt 7560 TCGTTATCGTAGTGTTTAAAGTGTTTATTTCGTAAAAAAAGTGACGTAAGATCAACACCA 7561 ttgtccaaactcatcaatgtatcttatcatgtctggcatctatgtcgggtgcggagaaag 7620 AACAGGTTTGAGTAGTTACATAGAATAGTACAGACCGTAGATACAGCCCACGCCTCTTTC 7621 aggtaatgaaatggcattatgggtattatgggtctgcattaatgaatcggccaacgatcc 7680 TCCATTACTTTACCGTAATACCCATAATACCCAGACGTAATTACTTAGCCGGTTGCTAGG 7681 cggtgtgaaataccgcacagatgcgtaaggagaaaataccgcatcaggcgctcttccgct 7740 GCCACACTTTATGGCGTGTCTACGCATTCCTCTTTTATGGCGTAGTCCGCGAGAAGGCGA 7741 tcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcac 7800 AGGAGCGAGTGACTGAGCGACGCGAGCCAGCAAGCCGACGCCGCTCGCCATAGTCGAGTG 7801 tcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtga 7860 AGTTTCCGCCATTATGCCAATAGGTGTCTTAGTCCCCTATTGCGTCCTTTCTTGTACACT pBR322 origin(7899,8518)<<< | 7861 gcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccat 7920 CGTTTTCCGGTCGTTTTCCGGTCCTTGGCATTTTTCCGGCGCAACGACCGCAAAAAGGTA 7921 aggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaac 7980 TCCGAGGCGGGGGGACTGCTCGTAGTGTTTTTAGCTGCGAGTTCAGTCTCCACCGCTTTG 7981 ccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcct 8040 GGCTGTCCTGATATTTCTATGGTCCGCAAAGGGGGACCTTCGAGGGAGCACGCGAGAGGA 8041 gttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcg 8100 CAAGGCTGGGACGGCGAATGGCCTATGGACAGGCGGAAAGAGGGAAGCCCTTCGCACCGC 8101 ctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctg 8160 GAAAGAGTATCGAGTGCGACATCCATAGAGTCAAGCCACATCCAGCAAGCGAGGTTCGAC 8161 ggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgt 8220 CCGACACACGTGCTTGGGGGGCAAGTCGGGCTGGCGACGCGGAATAGGCCATTGATAGCA 8221 cttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacagg 8280 GAACTCAGGTTGGGCCATTCTGTGCTGAATAGCGGTGACCGTCGTCGGTGACCATTGTCC 8281 attagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactac 8340 TAATCGTCTCGCTCCATACATCCGCCACGATGTCTCAAGAACTTCACCACCGGATTGATG 8341 ggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcgga 8400 CCGATGTGATCTTCCTGTCATAAACCATAGACGCGAGACGACTTCGGTCAATGGAAGCCT 8401 aaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggttttttt 8460 TTTTCTCAACCATCGAGAACTAGGCCGTTTGTTTGGTGGCGACCATCGCCACCAAAAAAA 8461 gtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttt 8520 CAAACGTTCGTCGTCTAATGCGCGTCTTTTTTTCCTAGAGTTCTTCTAGGAAACTAGAAA 8521 tctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgaga 8580 AGATGCCCCAGACTGCGAGTCACCTTGCTTTTGAGTGCAATTCCCTAAAACCAGTACTCT 8581 ttatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatc 8640 AATAGTTTTTCCTAGAAGTGGATCTAGGAAAATTTAATTTTTACTTCAAAATTTAGTTAG 8641 taaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacct 8700 ATTTCATATATACTCATTTGAACCAGACTGTCAATGGTTACGAATTAGTCACTCCGTGGA 8701 atctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagata 8760 TAGAGTCGCTAGACAGATAAAGCAAGTAGGTATCAACGGACTGAGGGGCAGCACATCTAT 8761 actacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagaccca 8820 TGATGCTATGCCCTCCCGAATGGTAGACCGGGGTCACGACGTTACTATGGCGCTCTGGGT 8821 cgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcaga 8880 GCGAGTGGCCGAGGTCTAAATAGTCGTTATTTGGTCGGTCGGCCTTCCCGGCTCGCGTCT 8881 agtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctaga 8940 TCACCAGGACGTTGAAATAGGCGGAGGTAGGTCAGATAATTAACAACGGCCCTTCGATCT 8941 gtaagtagttcgccagttaatagtttgcgcaacgttgttgaaaaaggatcttcacctaga 9000 CATTCATCAAGCGGTCAATTATCAAACGCGTTGCAACAACTTTTTCCTAGAAGTGGATCT 9001 tccttttcacgtagaaagccagtccgcagaaacggtgctgaccccggatgaatgtcagct 9060 AGGAAAAGTGCATCTTTCGGTCAGGCGTCTTTGCCACGACTGGGGCCTACTTACAGTCGA 9061 actgggctatctggacaagggaaaacgcaagcgcaaagagaaagcaggtagcttgcagtg 9120 TGACCCGATAGACCTGTTCCCTTTTGCGTTCGCGTTTCTCTTTCGTCCATCGAACGTCAC NEOKAN prom(9171,9220)>>> | 9121 ggcttacatggcgatagctagactgggcggttttatggacagcaagcgaaccggaattgc 9180 CCGAATGTACCGCTATCGATCTGACCCGCCAAAATACCTGTCGTTCGCTTGGCCTTAACG 9181 cagctggggcgccctctggtaaggttgggaagccctgcaaagtaaactggatggctttct 9240 GTCGACCCCGCGGGAGACCATTCCAACCCTTCGGGACGTTTCATTTGACCTACCGAAAGA BclI | 9241 cgccgccaaggatctgatggcgcaggggatcaagctctgatcaagagacaggatgaggat 9300 GCGGCGGTTCCTAGACTACCGCGTCCCCTAGTTCGAGACTAGTTCTCTGTCCTACTCCTA NTP_II marker(9312,10100)>>> | ORF_4 rf(3)(9309,10103)>>> | | 9301 cgtttcgcatgattgaacaagatggattgcacgcaggttctccggccgcttgggtggaga 9360 GCAAAGCGTACTAACTTGTTCTACCTAACGTGCGTCCAAGAGGCCGGCGAACCCACCTCT 9361 ggctattcggctatgactgggcacaacagacaatcggctgctctgatgccgccgtgttcc 9420 CCGATAAGCCGATACTGACCCGTGTTGTCTGTTAGCCGACGAGACTACGGCGGCACAAGG 9421 ggctgtcagcgcaggggcgcccggttctttttgtcaagaccgacctgtccggtgccctga 9480 CCGACAGTCGCGTCCCCGCGGGCCAAGAAAAACAGTTCTGGCTGGACAGGCCACGGGACT 9481 atgaactgcaagacgaggcagcgcggctatcgtggctggccacgacgggcgttccttgcg 9540 TACTTGACGTTCTGCTCCGTCGCGCCGATAGCACCGACCGGTGCTGCCCGCAAGGAACGC 9541 cagctgtgctcgacgttgtcactgaagcgggaagggactggctgctattgggcgaagtgc 9600 GTCGACACGAGCTGCAACAGTGACTTCGCCCTTCCCTGACCGACGATAACCCGCTTCACG 9601 cggggcaggatctcctgtcatctcaccttgctcctgccgagaaagtatccatcatggctg 9660 GCCCCGTCCTAGAGGACAGTAGAGTGGAACGAGGACGGCTCTTTCATAGGTAGTACCGAC 9661 atgcaatgcggcggctgcatacgcttgatccggctacctgcccattcgaccaccaagcga 9720 TACGTTACGCCGCCGACGTATGCGAACTAGGCCGATGGACGGGTAAGCTGGTGGTTCGCT 9721 aacatcgcatcgagcgagcacgtactcggatggaagccggtcttgtcgatcaggatgatc 9780 TTGTAGCGTAGCTCGCTCGTGCATGAGCCTACCTTCGGCCAGAACAGCTAGTCCTACTAG 9781 tggacgaagagcatcaggggctcgcgccagccgaactgttcgccaggctcaaggcgagca 9840 ACCTGCTTCTCGTAGTCCCCGAGCGCGGTCGGCTTGACAAGCGGTCCGAGTTCCGCTCGT 9841 tgcccgacggcgaggatctcgtcgtgacccatggcgatgcctgcttgccgaatatcatgg 9900 ACGGGCTGCCGCTCCTAGAGCAGCACTGGGTACCGCTACGGACGAACGGCTTATAGTACC 9901 tggaaaatggccgcttttctggattcatcgactgtggccggctgggtgtggcggaccgct 9960 ACCTTTTACCGGCGAAAAGACCTAAGTAGCTGACACCGGCCGACCCACACCGCCTGGCGA 9961 atcaggacatagcgttggctacccgtgatattgctgaagagcttggcggcgaatgggctg 10020 TAGTCCTGTATCGCAACCGATGGGCACTATAACGACTTCTCGAACCGCCGCTTACCCGAC 10021 accgcttcctcgtgctttacggtatcgccgctcccgattcgcagcgcatcgccttctatc 10080 TGGCGAAGGAGCACGAAATGCCATAGCGGCGAGGGCTAAGCGTCGCGTAGCGGAAGATAG 10081 gccttcttgacgagttcttctgaattttgttaaaatttttgttaaatcagctcatttttt 10140 CGGAAGAACTGCTCAAGAAGACTTAAAACAATTTTAAAAACAATTTAGTCGAGTAAAAAA 10141 aaccaataggccgaaatcggcaacatcccttataaatcaaaagaatagaccgcgataggg 10200 TTGGTTATCCGGCTTTAGCCGTTGTAGGGAATATTTAGTTTTCTTATCTGGCGCTATCCC f1 origin(10208,10513)<<< | 10201 ttgagtgttgttccagtttggaacaagagtccactattaaagaacgtggactccaacgtc 10260 AACTCACAACAAGGTCAAACCTTGTTCTCAGGTGATAATTTCTTGCACCTGAGGTTGCAG 10261 aaagggcgaaaaaccgtctatcagggcgatggcccactacgtgaaccatcacccaaatca 10320 TTTCCCGCTTTTTGGCAGATAGTCCCGCTACCGGGTGATGCACTTGGTAGTGGGTTTAGT 10321 agttttttgcggtcgaggtgccgtaaagctctaaatcggaaccctaaagggagcccccga 10380 TCAAAAAACGCCAGCTCCACGGCATTTCGAGATTTAGCCTTGGGATTTCCCTCGGGGGCT 10381 tttagagcttgacggggaaagccggcgaacgtggcgagaaaggaagggaagaaagcgaaa 10440 AAATCTCGAACTGCCCCTTTCGGCCGCTTGCACCGCTCTTTCCTTCCCTTCTTTCGCTTT 10441 ggagcgggcgctagggcgctggcaagtgtagcggtcacgctgcgcgtaaccaccacaccc 10500 CCTCGCCCGCGATCCCGCGACCGTTCACATCGCCAGTGCGACGCGCATTGGTGGTGTGGG 10501 gcgcgcttaatgcgccgctacagggcgcgtccattcgccattcaggatcgaattaattct 10560 CGCGCGAATTACGCGGCGATGTCCCGCGCAGGTAAGCGGTAAGTCCTAGCTTAATTAAGA 10561 taattaacatcatcaataatatacctt 10587 ATTAATTGTAGTAGTTATTATATGGAA
LOCUS dna 10587 bp FEATURES Location/Qualifiers Other Gene 162..310 /gene="Encap other" Promoter 365..393 /gene="amp prom" misc_binding 457..462 /dbxref="REBASE:HpaI" misc_binding 457..462 /dbxref="REBASE:HincII" Other Gene 715..895 /gene="HIV-1_5_LTR other" Other Gene 1006..1050 /gene="HIV-1_psi_pack other" misc_binding 1092..1097 /dbxref="REBASE:NruI" Regulatory_Seq 1557..1790 /gene="RRE reg" ORF 1600..2301 /sequence="ORF_1 rf(1)" misc_binding 2611..2616 /dbxref="REBASE:NdeI" Promoter 2885..2966 /gene="CMV prom" Regulatory_Seq 3022..3061 /gene="tetO reg" ORF 3063..3749 /sequence="ORF_6 rf(5)" misc_binding 3074..3079 /dbxref="REBASE:ClaI" misc_binding 3098..3103 /dbxref="REBASE:KpnI" misc_binding 3125..3130 /dbxref="REBASE:EcoRI" ORF 3135..6533 /sequence="ORF_3 rf(3)" ORF 4170..5390 /sequence="ORF_5 rf(5)" misc_binding 5099..5104 /dbxref="REBASE:XhoI" misc_binding 5187..5192 /dbxref="REBASE:EcoRV" misc_binding 5623..5628 /dbxref="REBASE:BamHI" ORF 6533..7171 /sequence="ORF_2 rf(2)" misc_binding 6540..6547 /dbxref="REBASE:NotI" Other Gene 7160..7212 /gene="delta_U3 other" Other Gene 7213..7393 /gene="HIV-1_5_LTR other" Rep_Origin 7899..8518 /gene="pBR322 origin" Promoter 9171..9220 /gene="NEOKAN prom" misc_binding 9278..9283 /dbxref="REBASE:BclI" ORF 9309..10103 /sequence="ORF_4 rf(3)" Marker 9312..10100 /gene="NTP_II marker" Rep_Origin 10208..10513 /gene="f1 origin"